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1.
Mol Genet Genomics ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33025164

RESUMO

Reduced fertility is a common clinical feature of the individuals with Fanconi anemia (FA), a rare autosomal recessive disorder due to deficiency in FA pathway during DNA repair. Our previous study reported that the heterozygous pathogenic variants in FANCA (Fanconi anemia complementation group A) induced premature ovarian insufficiency (POI). However, the genotype-phenotype correlation in POI caused by FANCA variants remains considerably uncertain. Herein, a heterozygous non-frameshift Fanca-mutated mouse strain (Fanca+/hypo) carrying a 9-bp deletion (c.3581del9, p.QEA1194-1196del) was generated. The mutant mice exhibited slightly decreased Fanca protein level in ovaries, suggesting the non-frameshift deletion mutant is hypomorphic. Female fertility test showed decreased number of litters, litter sizes and prolonged litter interval time in the female Fanca+/hypo mice compared to wild-type mice. Follicle counting revealed a consistent decreasing pattern of follicle numbers in Fanca+/hypo females compared to that in wild-type mice with aging. Furthermore, embryonic fibroblasts of Fanca+/hypo mice were hyper-responsive to Mitomycin C in vitro, demonstrating a partial loss of function of this hypomorphic Fanca mutant in DNA repair. Collectively, our experimental observations suggest that the hypomorphic Fanca allele is sufficient to reduce female fertility in mice, providing new insights into the genetic counseling of FANCA variants in subfertile women.

2.
Biomater Sci ; 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33000773

RESUMO

Over the past few years, tissue-engineering technology provided a new direction for bone defects therapy, which involved developing applicable biological materials composite with seed cells to repair bone defects tissue. However, as one of the commonest seed cells for tissue engineering, BMSCs (bone marrow mesenchymal stem cells), are still lacking an efficient and accurate differentiation ability into functional osteoblast. Given these facts, the development of a novel tissue engineering technology integrated BMSCs and scaffold materials have become an urgent need for bone defects repair. In this work, we found that miR-19b-3p could suppress the expression of Smurf1 which is a negative regulator of osteogenesis. By employing lentivirus pLVTHM-miR-19b-3p transfected BMSCs, we verified that miR-19b-3p could promote BMSCs osteogenic differentiation via suppressing Smurf1 expression. Furthermore, we designed a new porous PLLA/POSS scaffold combined with BMSCs for tissue engineering. In vitro experiment showed that miR-19b-3p modified BMSCs facilitated the expansion and proliferation of BMSCs when culturing with the PLLA/POSS scaffold. We established rats calvarial critical-sized defect model, after transplanting the BMSCs/PLLA/POSS for 3 month, the pathology, immunohistochemical and Micro-CT results showed that miR-19b-BMSCs/PLLA/POSS significantly facilitated the osteogenesis differentiation, enhanced the bone density of defect area and accelerated the repair of bone defect. We elucidated the mechanism that miR-19b-3p suppressed the expression of Smurf1 and provided a novel tissue engineering strategy for using microRNA gene-modified BMSCs combined with PLLA/POSS scaffold in bone tissue engineering.

3.
Comput Methods Programs Biomed ; 198: 105766, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-33059061

RESUMO

BACKGROUND AND OBJECTIVE: Virus identification in electron microscopy (EM) images is considered as one of the front-line method in pathogen diagnosis and re-emerging infectious agents. However, the existing methods either focused on the detection of a single virus or required large amounts of manual labeling work to segment virus. In this work, we focus on the task of virus classification and propose an effective and simple method to identify different viruses. METHODS: We put forward a residual mixed attention network (RMAN) for virus classification. The proposed network uses channel attention, bottom-up and top-down attention, and incorporates a residual architecture in an end-to-end training manner, which is suitable for dealing with EM virus images and reducing the burden of manual annotation. RESULTS: We validate the proposed network through extensive experiments on a transmission electron microscopy virus image dataset. The top-1 error rate of our RMAN on 12 virus classes is 4.285%, which surpasses that of state-of-the-art networks and even human experts. In addition, the ablation study and the visualization of class activation mapping (CAM) further demonstrate the effectiveness of our method. CONCLUSIONS: The proposed automated method contributes to the development of medical virology, which provides virologists with a high-accuracy approach to recognize viruses as well as assist in the diagnosis of viruses.

4.
Lipids Health Dis ; 19(1): 221, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33046100

RESUMO

BACKGROUNDS: Stroke is a sudden disorder of cerebral blood circulation. Many studies have illustrated that dyslipidemia, hypertension, diabetes, smoking and excessive drinking are the traditional risk factors for stroke. This study aimed to observe the relationship between CYP1A1 and CYP1A2 variants and stroke risk in the Chinese population. METHODS: Agena MassARRAY Assay was used to genotype four single nucleotide polymorphisms (SNPs) in 477 cases and 480 controls. The chi-square test and logistic-regression analysis were used to explore the relationship between CYP1A1 and CYP1A2 variants and stroke risk. RESULTS: Individuals with CYP1A2 rs762551 C was associated with a lower risk of stroke than that of allele A. Age stratification analysis showed that rs762551 was only observed to be associated with a lower risk of stroke in ≤64ys age group. After gender stratification analysis, a significant association between rs762551 and stroke risk was found in males, but not in females. The four SNPs were found to be correlated with stroke risk in patients with hypertension, coronary heart disease, cerebral infarction and lacunar infarction. CONCLUSION: In this study, the results first showed that CYP1A1 and CYP1A2 variants were associated with stroke risk. Larger and well-designed studies are needed to confirm the results.

5.
Plant Dis ; 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33054622

RESUMO

A total of 707 isolates of Botrytis were collected from plastic tunnel-grown strawberry and tomato in Hubei province of China. They were identified based on the specific molecular markers. Diversity of the B. cinerea (Bc) isolates was evaluated by typing the transposable elements or TE (Boty, Flipper) and the mating types (MAT1-1, MAT1-2), as well as by determining virulence on tobacco (Nicotiana benthamiana) and fenhexamid sensitivity in agar medium. The results showed that 706 isolates (accounting for 99.9%) belonged to Bc, one isolate (accounting for 0.1%) belonged to B. pseudocinerea. The Bc isolates fell into four TE types, Vacuma, Boty, Flipper and Transposa, which accounted for 3.1%, 9.6%, 18.4% and 68.8% (n = 706), respectively. The strawberry and tomato sub-populations of Bc differed significantly (P < 0.05) in composition of the four TE types. The overall ratio of MAT1-1 to MAT1-2 deviated from 1:1 (n = 706, P = 0.0002), MAT1-2 (accounting for 56.9%) predominated over MAT1-1 (accounting for 43.1%). However, in 7 out of 12 geographic sub-populations, the ratio of MAT1-1 to MAT1-2 matched 1:1, whereas in the remaining five geographic sub-populations, the ratio of MAT1-1 to MAT1-2 did not match 1:1. Results of the biological characterizations showed that most Bc isolates were highly sensitive or sensitive to fenhexamid, and the majority of Bc isolates were highly virulent or virulent on tobacco. Moreover, relationship between genetic diversity and biological characteristics was analyzed. The results achieved in this study are helpful for understanding of the populations of B. cinerea.

6.
J Orthop Surg Res ; 15(1): 474, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33059739

RESUMO

BACKGROUND: Coronavirus disease 2019 (COVID-19) has broken out and spread rapidly nationwide at the beginning of 2020, which has brought huge impacts to people and work. The current situation of prevention and control is severe and urges guidance for clinicians, especially for medical systems. In the hope of providing a reference and recommendation for the prevention and control of the COVID-19, we carried out research to improve the quality of patient care and prevention during this epidemic. METHODS: All of the staff were trained rapidly to master personal protection in our department. We reviewed the patients' discharged records who underwent surgery in our department during January 1 to March 1, 2019, and January 1 to March 1, 2020. The management of the surgery patients and flow charts were described and analyzed. Post-operation outcomes of the patients include duration, complications, surgical site infection (SSI), system infection, re-operation, and mortality. Both chi-squared test and Student's t test were performed to determine the relationship between the two periods in terms of post-operation outcomes. RESULTS: Descriptive statistics analysis revealed that demographic of the patients between the two periods is similar. We had benefited from the strict flowcharts, smart robot, and protection equipment during the perioperative managements for orthopedic patients. With the help of the strict flow charts and smart equipment, post-operation outcomes of the patients revealed that the rates of the complications and re-operation had been reduced significantly (p < 0.05), while duration of operation, SSI, and system infection had no significant difference between two periods (p > 0.05). No patient and staff caught COVID-19 infection or mortality during the epidemic. CONCLUSIONS: Our study indicated that medical quality and efficiency were affected little with the help of strategies described above during the epidemic, which could be a reference tool for medical staff in routine clinical practice for admission of patients around the world. What is more, the provided strategies, which may evolve over time, could be used as empirical guidance and reference for orthopedic peers to get through the pandemic and ensure the normal operation of the hospital.

7.
Medicine (Baltimore) ; 99(37): e21963, 2020 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-32925730

RESUMO

This study aims to identify differentially expressed microRNAs (miRNAs) in gastric cancer by comparing gastric cancerous tissues with normal tissues, explore the potential roles.The miRNA expression microarray was employed on gastric cancer tissues, and apparently normal para-cancerous tissues from 3 patients undergoing radical surgery were matched. Quantitative RT-PCR was performed on the other 7 patients to validate the findings of the microarray. Furthermore, Gene Ontology (GO) analysis and enrichment analysis of KEGG Pathway were performed for 5 dysregulated candidate miRNAs, including 3 upregulated (miR-31-3p, miR-6736-3p, and miR-147b) and 2 downregulated (miR-3065-5p and miR-3921) miRNAs, in order to determine the role of miRNAs in tumorigenesis and development.Among these miRNAs, 17 miRNAs were found to be upregulated, and 19 miRNAs were found to be downregulated. The dysregulated expression of 5 candidate miRNAs, including miR-31-3p, miR-147b, miR-6736-3p, miR-3065-5p, and miR-3921, were verified by quantitative RT-PCR in the validation set. Among these miRNAs, miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921 had 551 target gene intersections. The GO and KEGG Pathway analyses Revealed that miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921 may participate in multiple pathophysiological processes, such as foreign substance metabolism and chemical carcinogenesis.The profile of differentially expressed miRNAs was successfully screened, and 4 miRNAs (i.e., miR-31-3p, miR-6736-3p, miR-3065-5p, and miR-3921) appeared to be involved in gastric carcinogenesis. These might serve as promising biomarkers for gastric cancer.


Assuntos
Perfilação da Expressão Gênica , MicroRNAs/genética , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/genética , Regulação para Baixo , Regulação Neoplásica da Expressão Gênica , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Regulação para Cima
8.
Nanomaterials (Basel) ; 10(9)2020 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-32825058

RESUMO

Broadband metamaterial absorbers (MAs) are critical for applications of photonic and optoelectronic devices. Despite long-standing efforts on broadband MAs, it has been challenging to achieve ultrabroadband absorption with high absorptivity and omnidirectional characteristics within a comparatively simple and low-cost architecture. Here we design, fabricate, and characterize a novel compact Cr-based MA to achieve ultrabroadband absorption in the visible to near-infrared wavelength region. The Cr-based MA consists of Cr nanorods and Cr substrate sandwiched by three pairs of SiO2/Cr stacks. Both simulated and experimental results show that an average absorption over 93.7% can be achieved in the range of 400-1000 nm. Specifically, the ultrabroadband features result from the co-excitations of localized surface plasmon (LSP) and propagating surface plasmon (PSP) and their synergistic absorption effects, where absorption in the shorter and longer wavelengths are mainly contributed bythe LSP and PSP modes, respectively. The Cr-based MA is very robust to variations of the geometrical parameters, and angle-and polarization-insensitive absorption can be operated well over a large range of anglesunder both transverse magnetic(TM)- and transverse electric (TE)-polarized light illumination.

9.
Exp Parasitol ; 217: 107965, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32818513

RESUMO

Saturated salt floatation method is widely used for coccidian oocyst purification. However, the repeated procedures and inefficient oocysts recovery rate are a continuous challenge. This study aimed to investigate the best suitable floatation solution, along with optimal centrifugation speed and time for Eimeria tenella (E. tenella) oocyst and sporocyst purification. Different floatation solutions i-e, saturated salt, Sheather's sugar and sodium hypochlorite (NaClO) at 20-60% concentrations were used to purify oocyst. It was found that about 96.99% oocysts (8609×g for 10 min) were recovered under these conditions without any effect on the viability of sporocysts. The recovery rate of oocysts using 50% NaClO (V/V) was significantly higher than 35% saturated salt flotation solution (P < 0.05). The optimal method for purification of oocysts based our experimentation was centrifugation at 8609×g for 3 min using 50% NaClO floatation solution, and the optimized centrifugation conditions for improved recovery of sporocysts (about 99.3%) were at 2152×g for 5 min. The present study provided a better method for the coccidian oocyst purification, which could be successfully adopted as a better alternative to existing techniques commonly used for investigations/research pertaining to coccidia.


Assuntos
Centrifugação/normas , Eimeria tenella/isolamento & purificação , Análise de Variância , Animais , Galinhas , Eimeria tenella/crescimento & desenvolvimento , Fezes/parasitologia , Oocistos/isolamento & purificação , Oxidantes/administração & dosagem , Distribuição Aleatória , Hipoclorito de Sódio/administração & dosagem , Organismos Livres de Patógenos Específicos , Fatores de Tempo
10.
Biomed Res Int ; 2020: 2790679, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32685459

RESUMO

Circular RNAs (circRNAs), as a recently established group of endogenous noncoding RNAs, have been involved in the occurrence and development of different malignancies. Gastric cancer (GC) remains a globally significant contributor to death in cancer patients due to insufficient early diagnosis, limited treatment measures, and poor prognosis. An increasing number of studies have found that many circRNAs are dysregulated in GC and are closely associated with its tumorigenesis and metastasis. Thus, circRNAs have the potential to serve as diagnostic and prognostic biomarkers and even therapeutic targets. This review comprehensively summarizes the most recent findings on how circRNAs influence GC progression and their clinical value. In addition, we present several methological deficiencies in the studies and provide some promising ideas for future research.

11.
Autoimmunity ; 53(6): 297-302, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32543888

RESUMO

LncRNA PlncRNA-1(PlncRNA-1) participates in breast cancer by upregulating TGF-ß1. It is known that TGF-ß1 plays an inhibitory role in the inflammatory responses in rheumatoid arthritis (RA). Therefore, PlncRNA-1 may also participate in RA. Serum and synovial fibroblasts were obtained from 34 patients with active RA (persistent symptoms), 36 patients with inactive RA (long term of no or few symptoms after active RA) and 40 healthy controls. Expression levels of PlncRNA-1 and TGF-ß1 in active RA patients, inactive RA patients and healthy controls were measured by RT-qPCR and ELISA, respectively. Pearson Correlation Coefficient was used to determine the correlation between the expression levels of PlncRNA-1 and TGF-ß1. Diagnostic value of PlncRNA-1 for active RA was detected by ROC curve analysis. PlncRNA-1 and TGF-ß1 were downregulated in serum of active RA patients but not in inactive RA patients compared to the healthy controls. Expression of PlncRNA-1 and TGF-ß1 were positively correlated only in RA patients, and altered expression levels of PlncRNA-1 distinguished the active RA patients from inactive RA patients and healthy controls. PlncRNA-1 and TGF-ß1 were also downregulated in synovial fibroblasts derived from RA patients in comparison to inactive RA patients and healthy controls. Overexpression of PlncRNA-1 mediated upregulation of TGF-ß1 in synovial fibroblasts derived from RA patients, while exogenous TGF-ß1 treatment showed no significant effect on the expression of PlncRNA-1. Therefore, PlncRNA-1 participated in RA possibly by regulating TGF-ß1.

12.
J Cell Sci ; 133(13)2020 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-32503940

RESUMO

The Cep63-Cep152 complex located at the mother centriole recruits Plk4 to initiate centriole biogenesis. How the complex is targeted to mother centrioles, however, is unclear. In this study, we show that Cep57 and its paralog, Cep57l1, colocalize with Cep63 and Cep152 at the proximal end of mother centrioles in both cycling cells and multiciliated cells undergoing centriole amplification. Both Cep57 and Cep57l1 bind to the centrosomal targeting region of Cep63. The depletion of both proteins, but not either one, blocks loading of the Cep63-Cep152 complex to mother centrioles and consequently prevents centriole duplication. We propose that Cep57 and Cep57l1 function redundantly to ensure recruitment of the Cep63-Cep152 complex to the mother centrioles for procentriole formation.

13.
Parasit Vectors ; 13(1): 248, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32404185

RESUMO

BACKGROUND: Sparganosis caused by Spirometra erinaceieuropaei spargana is a zoonotic parasitic infection that has been reported in many countries, including China, Japan, Thailand and Korea, as well as European countries and the USA. The biological and clinical significance of the parasite have previously been reported. Although the genomic and transcriptomic analysis of S. erinaceieuropaei provided insightful views about the development and pathogenesis of this species, little knowledge has been acquired in terms of post-translational regulation that is essential for parasite growth, development and reproduction. Here, we performed site-specific phosphoproteomic profiling, with an aim to obtain primary information about the global phosphorylation status of spargana. RESULTS: A total of 3228 phosphopeptides and 3461 phosphorylation sites were identified in 1758 spargana proteins. The annotated phosphoproteins were involved in a variety of biological pathways, including cellular (28%), metabolic (20%) and single-organism (17%) processes. The functional enrichment of phosphopeptides by Gene Ontology analysis indicated that most spargana phosphoproteins were related to the cytoskeleton cellular compartment, signaling molecular function, and a variety of biological processes, including a molecular function regulator, guanyl-nucleotide exchange factor activity, protein kinase activities, and calcium ion binding. The highly enriched pathways of phosphorylation proteins include the phosphatidylinositol signaling system, phagosome, endocytosis, inositol phosphate metabolism, terpenoid backbone biosynthesis, and peroxisome. Domain analysis identified an EF-hand domain and pleckstrin homology domain among the key domains. CONCLUSIONS: To our knowledge, this study performed the first global phosphoproteomic analysis of S. erinaceieuropaei. The dataset reported herein provides a valuable resource for future studies on the signaling pathways of this important zoonotic parasite.

14.
Gene ; 751: 144779, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32428697

RESUMO

In insects, nuclear receptors (NRs) including EcR (NR1H1), USP (NR2B4), E75 (NR1D3), HR3 (NR1F), HR4 (NR6) and FTZ-F1 (NR5A3) mediate the 20-hydroxyecdysone (20E) signaling cascade to play a critical role during larval metamorphosis. In this present paper, we focused on hormone receptor 38 (HR38) in Leptinotarsa decemlineata, the only insect homolog of the NR4A subclass. RNA interference (RNAi) of LdHR38 in the penultimate (third) instar larvae reduced the expression of an ecdysteroidogenesis gene and declined the titer of 20E. Knockdown of LdHR38 intensified the expression of LdUSP, LdE75, LdE74, LdE93, LdBroad and LdHR3, whereas repressed the transcription of LdFTZ-F1. Disruption of 20E signaling inhibited chitin biosynthesis in the larval cuticle. Approximately 25% of the LdHR38 RNAi larvae died, around 40% of the resultant larvae remained as prepupae or become deformed pupae. The body surface of the HR38 depleted abnormal prepupae and pupae looked wet, just like the cuticle being covered with a layer of liquid. Moreover, the increase of larval mortality, and the impairment of pupation and emergence exhibited dose-dependent manners. Furthermore, silencing LdHR38 at the final (fourth) instar caused similar but less severe impairment of pupation. Dietary supplement with 20E for the third instar larvae did not rescue the high larval death and only slightly alleviated the low pupation rate in the LdHR38 RNAi hypomorphs. Accordingly, we propose that HR38 is necessary for tune of ecdysteroidogenesis and for mediation of 20E signaling during metamorphosis in L. decemlineata.


Assuntos
Besouros/crescimento & desenvolvimento , Proteínas de Insetos/fisiologia , Metamorfose Biológica , Receptores Citoplasmáticos e Nucleares/fisiologia , Animais , Quitina/biossíntese , Besouros/genética , Besouros/metabolismo , Ecdisterona/fisiologia , Proteínas de Insetos/antagonistas & inibidores , Proteínas de Insetos/genética , Larva/genética , Larva/crescimento & desenvolvimento , Larva/metabolismo , Pupa/genética , Pupa/crescimento & desenvolvimento , Pupa/metabolismo , Interferência de RNA , Receptores Citoplasmáticos e Nucleares/antagonistas & inibidores , Receptores Citoplasmáticos e Nucleares/genética , Transdução de Sinais
15.
Bioresour Technol ; 312: 123565, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32454439

RESUMO

Coexistence of multiple pollutants such as antibiotic, nitrate and heavy metal has received increasing attention resently. In this study, the functions of Pseudomonas sp.H117 on the removal of tetracycline(TC), nitrate and Mn(II), and biological materials (BMO(biogenic manganese oxides), MBMO(magnetic BMO)) on the removal of TC were investigated. Strain H117 showed higher TC removal efficiency of 68.86% (0.071 mg·L-1·h-1) within 96 h. Meanwhile, NO3-N and Mn(II) achieved high removal efficiency of 100% (0.211 mg·L-1·h-1) and 64.64% (0.265 mg·L-1·h-1), respectively. Furthermore, trapping experiments testified that Mn(III) intermediate formed during the biological manganese oxidation process, which contribute to the TC degradation. 91.29% and 96.63% of TC removal efficiency within 12 h were achieved by BMO and MBMO. Moreover, XPS, FTIR spectra, kinetics analysis and adsorption isotherms elucidated Mn(III) oxidation, chemical adsorption and ligand exchange reactions contribute to the removal of TC by biomaterials.


Assuntos
Materiais Biocompatíveis , Pseudomonas , Adsorção , Antibacterianos , Compostos de Manganês , Oxirredução , Óxidos , Tetraciclina
16.
Biol Trace Elem Res ; 2020 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-32449009

RESUMO

Bone marrow mesenchymal stem cells (MSCs) are multipotent stem cells with the ability to differentiate into bone-producing cells, which is essential for bone formation. Magnesium biomedical materials, such as biodegradable matters with osteoinductive properties, play a vital role in the osteogenic differentiation of MSCs. International and Chinese studies have shown that magnesium ions, which are produced by biodegradation, mainly achieve this effect by regulating the expression of genes and proteins associated with osteogenesis, activating multiple signal pathways, elevating autophagic activities, and adjusting the pH in the microenvironment. It is of great significance to study the regulatory mechanisms and identify the optimal conditions that how magnesium ions promote osteogenic differentiation of MSCs. In this study, we summarized the regulatory mechanisms noted above.

17.
Ann Palliat Med ; 9(4): 1834-1840, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32279513

RESUMO

BACKGROUND: An association between left ventricular end-diastolic pressure (LVEDP) and outcomes of ischemic heart diseases has been reported. The present study aimed to investigate the LVEDP patterns and the effecting factors in patients with acute ST-segment elevation myocardial infarction (STEMI). METHODS: A total of 515 STEMI patients receiving immediate percutaneous coronary intervention (PCI) were divided into two groups according to their LVEDP before left ventricular angiography: LVEDP of 15 mmHg or less (group A, n=145) and LVEDP above 15 mmHg (group B, n=370). Blood samples were collected before and within 24 hours after PCI, and an ultrasonic cardiogram was conducted to measure left ventricular ejection fraction (EF%) and to evaluate ventricular structure changes. The narrowness of each artery was measured with coronary angiography. RESULTS: In comparison with group A, patients in group B had a more infarction-related artery (IRA) descending branch and regional wall motion abnormality, a larger left atrial end-diastolic diameter (LAEDd) and a left ventricular end-diastolic diameter (LVEDd), a smaller EF%, a higher level of myocardial necrosis markers, and a higher heart failure rate. Furthermore, LVEDP level was found to be positively correlated with Gensini score, LAEDd, LVEDd, N-terminal pro b-type natriuretic peptide, troponin T, uric acid, creatine kinase (CK), CK myocardial band, low-density lipoprotein cholesterol and fasting blood glucose, and negatively correlated with glomerular filtration rate and EF%. CONCLUSIONS: LVEDP elevation has a higher incidence of heart failure and a higher risk of death, which is associated with the criminal blood vessels.

18.
Medicine (Baltimore) ; 99(17): e19959, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32332681

RESUMO

This study aimed to investigate the association between Serum Uric Acid (UA) to Creatinine (Cr) Ratio (UA/Cr) and metabolic syndrome (MetS) in postmenopausal women.A total of 455 patients with MetS and 457 age- and gender- matched controls were included in the present retrospective study. Serum levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), Cr, and UA were measured. We employed logistic regression analysis to investigate the association between serum UA/Cr and MetS in postmenopausal women.Serum UA/Cr levels were significantly higher in patients with MetS than that in control subjects (P < .05). In the correlation analysis, serum UA/Cr showed a significantly positive correlation with age, hypertension, systolic pressure (SBP), diastolic pressure (DBP), Waist, body mass index (BMI), TG, UA and negative correlation with type 2 diabetes mellitus (T2DM) and Cr (P all < .001). Moreover, multivariate analysis revealed that serum UA/Cr was still an independent risk factor for MetS (OR = 2.928, 95% CI = 2.385-3.596, P < .001) after adjustments for other confounders.Serum UA/Cr are strongly associated with the risk of MetS in postmenopausal Chinese women.


Assuntos
Creatinina/análise , Síndrome Metabólica/sangue , Ácido Úrico/análise , Idoso , Índice de Massa Corporal , China , Creatinina/sangue , Feminino , Humanos , Modelos Logísticos , Masculino , Síndrome Metabólica/induzido quimicamente , Síndrome Metabólica/fisiopatologia , Pessoa de Meia-Idade , Pós-Menopausa/sangue , Estudos Retrospectivos , Fatores de Risco , Ácido Úrico/sangue , Circunferência da Cintura
19.
Parasitol Res ; 119(6): 1777-1784, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32300877

RESUMO

Ancylostoma ceylanicum is a zoonotic parasitic nematode that can cause iron-deficiency anemia and malnutrition in humans. A. ceylanicum hookworm platelet inhibitor (Ace-HPI) can inhibit platelet aggregation in the host to facilitate blood sucking, but whether it possesses platelet adhesion inhibitory activity or immunomodulatory role is yet unknown. To explore the effect of Ace-HPI on platelet adhesion, we expressed the recombinant protein in two competent cells, BL21 (DE3) and Rosetta-gami2 (DE3), and incubated this protein with canine platelets in a 96-well microplate. Ace-HPI was used to stimulate peripheral blood mononuclear cells (PBMC) in vitro to investigate the effect on PBMC proliferation and cytokine expression. Results showed that Ace-HPI expressed in Rosetta-gami2 (DE3) strain was mostly soluble. The inhibitory effect of this protein on platelet adhesion was relatively weak (7-8%). This protein stimulated the proliferation of PBMC and promoted the expression of Treg and Th2 cytokines, such as IL-10 and IL-13. These results lay a foundation for exploring the role of Ace-HPI in hookworm disease pathogenesis and as a candidate molecule for hookworm vaccines.


Assuntos
Ancylostoma/química , Proliferação de Células/efeitos dos fármacos , Proteínas de Helminto/farmacologia , Adesividade Plaquetária/efeitos dos fármacos , Inibidores da Agregação de Plaquetas/farmacologia , Ancylostoma/genética , Animais , Citocinas/metabolismo , Cães , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Inibidores da Agregação de Plaquetas/isolamento & purificação , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacologia
20.
Int J Oncol ; 56(4): 867-878, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32319549

RESUMO

Glutathione S­transferase Pi (GSTP1) is an isozyme encoded by the GST pi gene that plays an important regulatory role in detoxification, anti­oxidative damage, and the occurrence of various diseases. The aim of the present study was to review the association between the expression of GSTP1 and the development and treatment of various cancers, and discuss GSTP1 methylation in several malignant tumors, such as prostate, breast and lung cancer, as well as hepatocellular carcinoma; to review the association between polymorphism of the GSTP1 gene and various diseases; and to review the effects of GSTP1 on electrophilic oxidative stress, cell signal transduction, and the regulation of carcinogenic factors. Collectively, GSTP1 plays a major role in the development of various diseases.

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