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1.
Chin Med J (Engl) ; 133(1): 9-16, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31923099

RESUMO

BACKGROUND: The effects of keto acid (KA) supplements on Chinese patients receiving maintenance hemodialysis (MHD) are unclear. This study aimed to evaluate the effects of KA supplementation on nutritional status, inflammatory markers, and bioelectric impedance analysis (BIA) parameters in a cohort of Chinese patients with MHD without malnutrition. METHODS: This was a prospective, randomized, controlled, single-center clinical study conducted in 2011 till 2014. Twenty-nine patients with MHD were randomly assigned to a control (n = 14) or a KA (n = 15) group. The control group maintained a dietary protein intake of 0.9 g/kg/day. The KA group received additional KA supplement (0.1 g/kg/day). BIA was used to determine the lean tissue mass, adipose tissue mass, and body cell mass. The patients' nutritional status, dialysis adequacy, and biochemical parameters were assessed at the ends of the third and sixth months with t test or Wilcoxon rank-sum test. RESULTS: The daily total energy intake for both groups was about 28 kcal/kg/day. After 6 months, the Kt/V (where K is the dialyzer clearance of urea, t is the dialysis time, and V is the volume of the distribution of urea) was 1.33 ±â€Š0.25 in KA group, and 1.34 ±â€Š0.25 in the control group. The median triceps skin-fold thickness in KA group was 12.00 and 9.00 mm in the control group. In addition, the median hand-grip strength in KA group was 21.10 and 25.65 kg in the control group. There were no significant differences between the groups with respect to the anthropometry parameters, dialysis adequacy, serum calcium and phosphorus levels, inflammatory markers, and amino-acid profiles, or in relation to the parameters determined by BIA. Both groups achieved dialysis adequacy and maintained nutritional status during the study. CONCLUSIONS: In this cohort of Chinese patients with MHD, the patients in the control group whose dietary protein intake was 0.9 g/kg/day and total energy intake was 28 kcal/kg/day, maintained well nutritional status during study period. The KA supplement (0.1 g/kg/day) did not improve the essential amino acid/non-essential amino acid ratio, nor did it change the patients' mineral metabolism, inflammatory parameters, or body compositions.

2.
Chin Med J (Engl) ; 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31833909

RESUMO

BACKGROUND: The effects of keto acid (KA) supplements on Chinese patients receiving maintenance hemodialysis (MHD) are unclear. This study aimed to evaluate the effects of KA supplementation on nutritional status, inflammatory markers, and bioelectric impedance analysis (BIA) parameters in a cohort of Chinese patients with MHD without malnutrition. METHODS: This was a prospective, randomized, controlled, single-center clinical study conducted in 2011 till 2014. Twenty-nine patients with MHD were randomly assigned to a control (n = 14) or a KA (n = 15) group. The control group maintained a dietary protein intake of 0.9 g/kg/day. The KA group received additional KA supplement (0.1 g/kg/day). BIA was used to determine the lean tissue mass, adipose tissue mass, and body cell mass. The patients' nutritional status, dialysis adequacy, and biochemical parameters were assessed at the ends of the third and sixth months with t test or Wilcoxon rank-sum test. RESULTS: The daily total energy intake for both groups was about 28 kcal/kg/day. After 6 months, the Kt/V (where K is the dialyzer clearance of urea, t is the dialysis time, and V is the volume of the distribution of urea) was 1.33 ±â€Š0.25 in KA group, and 1.34 ±â€Š0.25 in the control group. The median triceps skin-fold thickness in KA group was 12.00 and 9.00 mm in the control group. In addition, the median hand-grip strength in KA group was 21.10 and 25.65 kg in the control group. There were no significant differences between the groups with respect to the anthropometry parameters, dialysis adequacy, serum calcium and phosphorus levels, inflammatory markers, and amino-acid profiles, or in relation to the parameters determined by BIA. Both groups achieved dialysis adequacy and maintained nutritional status during the study. CONCLUSIONS: In this cohort of Chinese patients with MHD, the patients in the control group whose dietary protein intake was 0.9 g/kg/day and total energy intake was 28 kcal/kg/day, maintained well nutritional status during study period. The KA supplement (0.1 g/kg/day) did not improve the essential amino acid/non-essential amino acid ratio, nor did it change the patients' mineral metabolism, inflammatory parameters, or body compositions.

3.
Medicine (Baltimore) ; 98(52): e18521, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31876744

RESUMO

Genetic testing of children is faced with numerous problems. High-quality clinical practice guidelines (CPGs) are needed to ensure its safe, and appropriate use. This study aimed to systematically identify the current CPGs for genetic testing in children, and to assess the methodological quality of these CPGs.We searched 6 databases, 3 guideline clearinghouses, and 9 web sites of relevant academic agencies from inception to February 2019. CPGs focused on genetic testing in children were included. Four reviewers independently appraised the quality of the eligible CPGs using the appraisal of guidelines for research, and evaluation (AGREE) II instrument.Seventeen CPGs meeting our inclusion criteria were included. Among them, 16 CPGs were focused on the genetic diagnosis/evaluation of diseases, while only 1 CPG was focused on pharmacogenetics. The median domain scores from highest to lowest were: scope and purpose 80.56% (range: 56.95%-87.50%), clarity of presentation 72.22% (range: 45.83%-88.89%), stakeholder involvement 45.83% (range: 27.78%-55.56%), applicability 31.25% (range: 19.79%-54.17%), rigor of development 21.88%, (range: 13.02%-71.88%), and editorial independence 18.75% (range: 0%-83.33%). According to the overall quality, 6 (35%) CPGs were "not recommended," 8 (47%) CPGs were "recommended with modifications," and only 3 (18%) CPGs were "recommended." The clinical topics of the "recommended" CPGs were warfarin, familial Mediterranean fever, and pediatric pulmonary arterial hypertension.The quality of CPGs for genetic testing in children was generally low, and variable across different CPGs and different AGREE II domains. In future guideline development, more attention should be paid to the aspects of stakeholder involvement, rigor of development, applicability, and editorial independence. Not only will guideline users benefit from our results when determining whether to adopt related CPGs to guide genetic testing in children, but guideline developers could also take into account our results to improve the quality of future CPGs.


Assuntos
Testes Genéticos/normas , Guias de Prática Clínica como Assunto/normas , Adolescente , Criança , Pré-Escolar , Humanos , Lactente , Recém-Nascido , Farmacogenética/normas
4.
Chin Med J (Engl) ; 132(20): 2395-2401, 2019 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-31651514

RESUMO

BACKGROUND: Determining the Helicobacter pylori (H. pylori) infection state during the gastroscopic process is important but still challenging. The linked color imaging (LCI) technique might emphasize the mucosal color change after H. pylori infection, which might help the diagnosis. In the present study, we aimed to compare the LCI technique with traditional white light imaging (WLI) endoscopy for diagnosing active H. pylori infection. METHODS: We collected and analyzed gastroscopic images from 103 patients in our hospital from November 2017 to March 2018, including both LCI and WLI modes. All images were randomly disordered and independently evaluated by four endoscopists who were blinded to the H. pylori status of patients. In addition, the H. pylori state was determined by both rapid urease test and pathology staining. The sensitivity, specificity, positive prediction value (PPV), and negative prediction value (NPV) were calculated for the detection of H. pylori infection. Moreover, the kappa value and interclass correlation coefficient (ICC) were used to evaluate the inter-observer variety by SPSS 24.0 software. RESULTS: Of the 103 enrolled patients, 27 of them were positive for H. pylori infection, while the 76 patients were negative. In total, 388 endoscopic images were selected, including 197 WLI and 191 LCI. The accuracy rate for H. pylori evaluation in the corpus LCI group was significantly higher than other groups (81.2% vs. 64.3%-76.5%, χ = 34.852, P < 0.001). Moreover, the corpus LCI group had the optimal diagnostic power with the sensitivity of 85.41% (95% confidence interval [CI]: 76.40%-91.51%), the specificity of 79.71% (95% CI: 74.38%-84.19%), the PPV of 59.42% (95% CI: 50.72%-67.59%), and the NPV of 94.02% (95% CI: 89.95%-96.56%), respectively. The kappa values between different endoscopists were higher with LCI than with WLI (0.433-0.554 vs. 0.331-0.554). Consistently, the ICC value was also higher with LCI than with WLI (0.501 [95% CI: 0.429-0.574] vs. 0.397 [95% CI: 0.323-0.474]). We further analyzed the factors that might lead to misjudgment, revealing that active inflammation might disturb WLI judgment (accuracy rate: 58.70% vs. 76.16%, χ = 21.373, P < 0.001). Atrophy and intestinal metaplasia might affect the accuracy of the LCI results (accuracy rate: 66.96% vs. 73.47%, χ = 2.027; 68.42% vs. 73.53%, χ = 1.594, respectively); however, without statistical significance (P = 0.154 and 0.207, respectively). CONCLUSIONS: The application of LCI at the corpus to identify H. pylori infection is reliable and superior to WLI. The inter-observer variability is lower with LCI than with WLI. TRIAL REGISTRATION: Chinese Clinical Trial Registry: ChiCTR1800016730; http://www.chictr.org.cn/showproj.aspx?proj=28400.

5.
PLoS One ; 14(10): e0224562, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31661512

RESUMO

BACKGROUND: The benefits of volatile anesthetics in coronary artery bypass grafting (CABG) patients remain controversial. We aimed to conduct an updated meta-analysis to assess whether the use of volatile anesthetics during CABG could reduce mortality and other outcomes. METHODS: We searched eight databases from inception to June 2019 and included randomized controlled trials (RCTs) comparing the effects of volatile anesthetics versus total intravenous anesthesia (TIVA) in CABG patients. The primary outcomes were operative mortality and one-year mortality. The secondary outcomes included the length of stay in the intensive care unit (ICU) and hospital and postoperative safety outcomes (myocardial infarction, heart failure, arrhythmia, stroke, delirium, postoperative cognitive impairment, acute kidney injury, and the use of intra-aortic balloon pump (IABP) or other mechanical circulatory support). Trial sequential analysis (TSA) was performed to control for random errors. RESULTS: A total of 89 RCTs comprising 14,387 patients were included. There were no significant differences between the volatile anesthetics and TIVA groups in operative mortality (relative risk (RR) = 0.92, 95% confidence interval (CI): 0.68-1.24, p = 0.59, I2 = 0%), one-year mortality (RR = 0.64, 95% CI: 0.32-1.26, p = 0.19, I2 = 51%), or any of the postoperative safety outcomes. The lengths of stay in the ICU and hospital were shorter in the volatile anesthetics group than in the TIVA group. TSA revealed that the results for operative mortality, one-year mortality, length of stay in the ICU, heart failure, stroke, and the use of IABP were inconclusive. CONCLUSIONS: Conventional meta-analysis suggests that the use of volatile anesthetics during CABG is not associated with reduced risk of mortality or other postoperative safety outcomes when compared with TIVA. TSA shows that the current evidence is insufficient and inconclusive. Thus, future large RCTs are required to clarify this issue.

6.
Acta Pharmacol Sin ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31506573

RESUMO

Intestinal mucositis is a common side effect of anticancer regimens that exerts a negative impact on chemotherapy. Superoxide dismutase (SOD) is a potential therapy for mucositis but efficient product is not available because the enzyme is degraded following oral administration or induces an immune reaction after intravascular infusion. Multi-modified Stable Anti-Oxidant Enzymes® (MS-AOE®) is a new recombinant SOD with better resistance to pepsin and trypsin. We referred it as MS-SOD to distinguish from other SODs. In this study we investigated its potential to alleviate 5-FU-induced intestinal injury and the mechanisms. An intestinal mucositis model was established in C57/BL6 mice by 5-day administration of 5-FU (50 mg/kg every day, ip). MS-SOD (800 IU/10 g, ig) was given once daily for 9 days. 5-FU caused severe mucositis with intestinal morphological damage, bodyweight loss and diarrhea; MS-SOD significantly decreased the severity. 5-FU markedly increased reactive oxygen species (ROS) and inflammatory cytokines in the intestine which were ameliorated by MS-SOD. Furthermore, MS-SOD modified intestinal microbes, particularly reduced Verrucomicrobia, compared with the 5-FU group. In Caco2 cells, MS-SOD (250-1000 U/mL) dose-dependently decreased tBHP-induced ROS generation. In RAW264.7 cells, MS-SOD (500 U/mL) had no effect on LPS-induced inflammatory cytokines, but inhibited iNOS expression. These results demonstrate that MS-SOD can scavenge ROS at the initial stage of injury, thus play an indirect role in anti-inflammatory and barrier protein protection. In conclusion, MS-SOD attenuates 5-FU-induced intestinal mucositis by suppressing oxidative stress and inflammation, and influencing microbes. MS-SOD may exert beneficial effect in prevention of intestinal mucositis during chemotherapy in clinic.

7.
J Environ Manage ; 249: 109326, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31421482

RESUMO

Flooding regime is an important agronomic strategy, and widely applied in heavy metal-contaminated soil for controlling heavy metal uptake through biochemical processes. However, the dynamics of the microbial community in the rhizosphere of rice under different flooding systems are not well understood. In the present study, we investigated the dynamics between the microbial community and heavy metal ions under continuous flooding (CF) and intermittent flooding (IF) conditions to decipher the relationship between microbial community and environmental factors. The results showed that, for the complex Cd, Pb, and Zn contaminated soil under CF treatment did not significantly suppress Cd uptake, but promoted Pb and Ni accumulation into the rice root. Soil pH and bio-available phosphate content appeared to be the key factors impacting heavy metal mobility. When observing the microbial community in the rhizosphere, long term flooding resulted in an abundance of Anaeromyxobactersp., Geobacter and Desulfovibrio, and the abundance of these taxa displayed a significant relationship to Pb and Zn content of rice roots. From the study, we observed that the flooding regime could have a significant impact on concentrations of Cd, Pb and Ni in rice roots, and the different richness of SRB and FeRB may contribute to uptake of these heavy metals. In future work, the impact of Fe cycling on heavy metal bioavailability in the plant rhizosphere should be investigated further.


Assuntos
Microbiota , Oryza , Poluentes do Solo , Rizosfera , Solo
8.
Biol Trace Elem Res ; 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31338805

RESUMO

The original version of this article unfortunately contained a mistake. The correct title should be "The Effects of 50 nm Unmodified Nano-ZnO on Lipid Metabolism and Semen Quality in Male Mice". The original article has been corrected.

9.
J Biomol Struct Dyn ; : 1-14, 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-31345118

RESUMO

In this study, we aimed to identify critical factors associated with superoxide dismutase 2 (SOD2) in human keratinocytes through gene and protein expression profiling approaches. After recombinant SOD2 was exogenously added to culture media, we conducted serial OMICS studies, which included RNA sequencing analysis, integrated antibody-chip arrays, and the implementation of bioinformatics algorithms, in order to reveal genes and proteins that are possibly associated with SOD2 in keratinocytes. These approaches identified several novel genes and proteins in keratinocytes that are associated with exogenous SOD2. These novel genes included DCT, which was up-regulated, and CD38, GPR151, HCK, KIT, and AFP, which were down-regulated. Among them, CD38 and KIT were also predicted as hub proteins in PPI mappings. By integrating the datasets obtained from these complementary high-throughput OMICS studies and utilizing the strengths of each method, we obtained new insights into the functional role of externally added SOD2 in skin cells and into several critical genes that are thought to play important roles in SOD2-associated skin function. The approach used here could help contribute to our clinical understanding of SOD2-associated applications and may be broadly applicable to a wider range of diseases. Abbreviations SOD2 superoxide dismutase 2 DAVID the database for annotation, visualization and integrated discovery KEGG Kyoto Encyclopedia of Genes and Genomes PPI protein-protein interactions HTS High-throughput screening Communicated by Ramaswamy H. Sarma.

10.
Biol Trace Elem Res ; 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31264129

RESUMO

Fifty male mice were exposed to 50 nm unmodified nano-ZnO through intragastric administration for 90 days to detect the long-term effects of unmodified nano-ZnO in mice. Results showed that the blood glucose, serum follicle stimulating hormone, luteinizing hormone, testosterone, and estradiol were significantly decreased (p < 0.05). The serum triglyceride, total cholesterol, and low-density lipoprotein were significantly increased (p < 0.05). The semen quality of the 160 mg/kg·bw group were significantly lowered (p < 0.05). The liver and testis catalase and CuZn-SOD activities were significantly elevated (p < 0.05). The abilities of •OH inhibition in the livers and testes of the 160 mg/kg·bw group were significantly lowered (p < 0.05). The liver and testis MDA levels of the 160 mg/kg·bw group were significantly elevated (p < 0.05). Results indicate that exposure of nano-ZnO could induce lipid metabolism disorder, hyperlipidemia, and reproductive toxicity to male mice through oxidative injury.

11.
Iran J Parasitol ; 14(1): 106-110, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31123474

RESUMO

Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents' brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.

13.
Prep Biochem Biotechnol ; 49(6): 597-605, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30929602

RESUMO

A simple and accurate Nile Red fluorescent method was built to evaluate the lipid content of three different oleaginous yeasts by one standard curve. The staining of cells can be observed clearly by laser scanning confocal microscope, showing that Nile Red can enter into the cells of oleaginous yeasts easily. A series of conditions such as pretreating temperature, cell suspension concentration (OD600), staining time, Nile Red concentration and the type of suspension solvent were learnt systematically to obtain the optimal process parameters for Nile Red staining. After optimization, the fitting curve of Nile Red fluorescent method was established under suitable conditions (pretreating temperature: 50 °C, OD600: 1.0; staining time: 5 mins; Nile Red concentration: 1.0 µg/mL; suspension solvent: PBS) and it had a suitable correlation coefficient (R2 = 0.95) for lipid content measurement of different oleaginous yeasts. By this study, the possibility of lipid content determination of different oleaginous yeasts by one fitting curve can be proven and this will improve the efficiency of researches related to microbial lipid production.


Assuntos
Corantes Fluorescentes/química , Lipídeos/análise , Microscopia Confocal/métodos , Oxazinas/química , Leveduras/química , Cryptococcus/química , Lipomyces/química , Coloração e Rotulagem/métodos , Temperatura Ambiente , Trichosporon/química
14.
Bioresour Technol ; 283: 191-197, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30908983

RESUMO

Elephant grass (Pennisetum purpureum) acid hydrolysate was used as substrate for anaerobic digestion for the first time. Within short period (ten days), the organic materials (sugars and organic acids) in the elephant grass hydrolysate could be utilized efficiently for stable biogas production that the COD removal, biogas yield, and CH4 content were 91.3 ±â€¯2.0%, 0.561 ±â€¯0.014 m3/kg COD consumption, and 68.1 ±â€¯4.6%, respectively throughout this bioprocess. During anaerobic digestion, almost no volatile fatty acids (VFAs) was accumulated (merely <0.1 g/L acetic acid was found) and the outlet pH was very stable (7.3 ±â€¯0.1). Meanwhile, the furans including furfural and 5-hydroxymethylfurfural (HMF) existing in the inlet substrate could be degraded. After anaerobic digestion, the outlet effluent was treated by combination of Fe-C micro-electrolysis and Fenton reaction to remove 93.1% of residual COD and 98.6% of color. Considering the performance, cost, operation, and environmental influence, this technology is suitable for industrial treatment of waste elephant grass.


Assuntos
Biocombustíveis , Pennisetum/metabolismo , Anaerobiose , Reatores Biológicos , Ácidos Graxos Voláteis/metabolismo , Metano/metabolismo
15.
Cells ; 8(2)2019 02 24.
Artigo em Inglês | MEDLINE | ID: mdl-30813496

RESUMO

Although the causal relationship between Alzheimer's disease (AD) and iron overload remains unclear, iron dyshomeostasis or improper transport mechanisms are speculated to lead to the accumulation of this neurotoxic metal in the hippocampal formation and other cerebral areas related to neurodegenerative diseases, resulting in the formation of reactive oxygen species (ROS) and, ultimately, cell death. In this study, exposure to high dietary iron (HDI) revealed no significant difference in the number of iron-positive cells and iron content in the cortex and hippocampal region between wild-type (WT) and APP/PS1 mice; however, compared with the control mice, the HDI-treated mice exhibited upregulated divalent metal transporter 1 (DMT1) and ferroportin (Fpn) expression, and downregulated transferrin receptor (TFR) expression. Importantly, we confirmed that there were significantly fewer NeuN-positive neurons in both APP/PS1 and WT mice given HDI, than in the respective controls. Moreover, this iron-induced neuron loss may involve increased ROS and oxidative mitochondria dysfunction, decreased DNA repair, and exacerbated apoptosis and autophagy. Although HDI administration might trigger protective antioxidant, anti-apoptosis, and autophagy signaling, especially in pathological conditions, these data clearly indicate that chronic iron exposure results in neuronal loss due to apoptosis, autophagy, and ferroptosis, hence increasing the risk for developing AD.


Assuntos
Ferro na Dieta/farmacologia , Degeneração Neural/patologia , Peptídeos beta-Amiloides/metabolismo , Animais , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Transporte Biológico/efeitos dos fármacos , Encéfalo/metabolismo , Dano ao DNA , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Neuroglia/efeitos dos fármacos , Neuroglia/metabolismo , Neuroglia/patologia , Oxirredução , Presenilina-1/metabolismo
16.
Sci Total Environ ; 663: 447-452, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30716636

RESUMO

Wastewater treatment is one important issue for turpentine plant and more wastewater generated by greater turpentine processing will prevent its further development. To solve this issue without extra place and new equipment, one industrial system for reuse and treatment of turpentine processing wastewater was introduced for the first time. For wastewater reuse, the technology was simple and easy to control that after neutralization by lime and absorption with activated carbon (optional, mostly not necessary), the wastewater could be reused for turpentine processing. After reuse, the wastewater was further treated by a biological system. During long-term application of wastewater reuse in this plant, it showed little influence on the products performance (mainly acid value) and final wastewater COD. Base on above advantages, the plant could decide when for wastewater drainage, and thus the amount of wastewater was reduced greatly. For the biological treatment, the COD of wastewater could be degraded to suitable level stably and the wastewater after treatment could be applied for daily life in the plant. Overall, considering the cost, operation, and performance, the whole system shows great potential and possibility of industrial application and therefore can be applied widely in the turpentine processing industry.

17.
Carbohydr Polym ; 208: 421-430, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30658820

RESUMO

Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG). For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process. For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed. Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity. The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite. Overall, this study provides one method for superabsorbent synthesis from low-cost and natural resources.


Assuntos
Bactérias/classificação , Celulose/metabolismo , Argila/química , Bentonita/química , Celulose/química , Fermentação , Água/química
18.
Appl Biochem Biotechnol ; 188(3): 585-601, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30552624

RESUMO

An adsorption resin CX-6 was synthesized and used for acid soluble lignin (ASL) removal from sugarcane bagasse hydrolysate (SCBH). The adsorption conditions of pH value, amount of adsorbent, initial ASL concentration, and temperature on ASL adsorption were discussed. The results showed the adsorption capacity of ASL was negatively affected by increasing temperature, solution pH, and adsorbent dose, and was positively affected by increasing initial concentration. The maximum adsorption capacity of ASL was 135.3 mg/g at initial ASL concentration 6.46 g/L, adsorption temperature 298 K, and pH 1. Thermodynamic study demonstrated that the adsorption process was spontaneous and exothermic. Equilibrium and kinetics experiments were proved to fit the Freundlich isotherm model and pseudo-second-order model well, respectively. Fermentation experiment showed that the SCBH after combined overliming with resin adsorption as fermentation substrate for microbial lipid production by Trichosporon cutaneum and Trichosporon coremiiforme was as better as that of SCBH by combined overliming with active charcoal adsorption, and more efficient than that of SCBH only by overliming. Moreover, the regeneration experiment indicated that the CX-6 resin is easy to regenerate and its recirculated performance is stable. In conclusion, our results provide a promising adsorbent to detoxify lignocellulose hydrolysate for further fermentation.


Assuntos
Ácidos/química , Lignina/isolamento & purificação , Lipídeos/biossíntese , Saccharum/química , Adsorção , Fermentação , Concentração de Íons de Hidrogênio , Hidrólise , Lignina/química , Microscopia Eletrônica de Varredura , Solubilidade , Temperatura Ambiente , Termodinâmica , Trichosporon/metabolismo
19.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 40(5): 637-641, 2018 Oct 30.
Artigo em Chinês | MEDLINE | ID: mdl-30404695

RESUMO

Objective To evaluate the nutrition risks in Chinese elderly adults in community and provide the basis for malnutrition prevention.Methods The study population comprised of adults aged 60 years or older selected from communities. A stratified multi-stage cluster sampling method was used to investigate older adults in rural and urban areas with structured questionnaire. Nutrition Screening Initiative (NSI) checklist was used to screen nutritional status. Analyses were stratified according to age,gender,different regions,rural and urban areas,and income.Results A total of 3885 older adults with complete information were enrolled for final analyses,among whom 1894 (48.8%) were males and 1991 (51.2%) were females; 56.2% aged 60-69 years,28.8% aged 70-79 years,and 15% aged over 80 years; 1665(42.9%) were urban residents; 1592(41.0%) were in the eastern region,1211(31.2%) in the central region,and 1082(27.8%) in the western region. Up to 48.4% of the elderly adults were at high nutritional risk,and the nutritional risk was significantly higher in females (50.7%) than in males (46.0%),in individuals aged over 80 years (53.0%) than in other age groups,in urban area (41.7%) than in rural area (53.9%),and in eastern region (52.9%) than in other regions. Significant differences were found between nutritional status and the following variables: age (Χ 2=33.7,P=0.000),gender (Χ 2=15.7,P=0.000),different regions (Χ 2=72.0,P=0.000),rural and urban areas (Χ 2=69.4,P=0.000),income (Χ 2=304.9,P=0.000),and living arrangement (Χ 2=128.1,P=0.000).Conclusion Elder adults in community are at high nutritional risk.


Assuntos
Desnutrição/epidemiologia , Estado Nutricional , Idoso , Idoso de 80 Anos ou mais , Grupo com Ancestrais do Continente Asiático , China , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medição de Risco , População Rural , Inquéritos e Questionários , População Urbana
20.
Biomed Chromatogr ; 32(12): e4373, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30133708

RESUMO

The aim of the present study was to develop a rapid, specific and sensitive LC-MS/MS method for the determination of DPHB [7-(4″-hydroxy-3″-methoxyphenyl)-1-phenyl-4-hepten-3-one] in rat plasma using yakuchinone A as an internal standard (IS). n-Hexane was used for the extraction of DPHB from rat plasma. Chromatographic separation of DPHB was achieved using a Kinetex XB-C18 column (2.10 × 50 mm, 2.6 µm) at 40°C. The mobile phase consisted of water (containing 0.1‰ formic acid, A) and acetonitrile (containing 0.1‰ formic acid, B) under a gradient elution at a flow rate of 0.3 mL min-1 . Positive electrospray ionization and multiple reaction monitoring mode were used for detection. The selected precursor ion to product ion pairs, m/z 311.3 → 137.0 for DPHB and m/z 313.1 → 137.0 for yakuchinone A, were monitored. Good linearity was observed over the concentration range from 2 to 2000 ng mL-1 (r = 0.9969). The recovery efficiency of DPHB from rat plasma was 54.8-69.7%, while the matrix effect ranged from 99.7 to 113%. Intra- and inter-day precision and accuracy values were within ±15% at three different quality control concentration levels. This validated method was successfully applied to pharmacokinetic studies in rats after a single p.o. or i.v. dose of DPHB solution. The route of administration significantly influenced systemic exposure to DPHB, and low bioavailability of DPHB was observed. The method developed here will be further improved and used in future pharmacokinetic studies.


Assuntos
Cromatografia Líquida/métodos , Diarileptanoides/sangue , Espectrometria de Massas em Tandem/métodos , Animais , Diarileptanoides/química , Diarileptanoides/farmacocinética , Estabilidade de Medicamentos , Modelos Lineares , Masculino , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
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