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1.
Chem Biodivers ; 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34643056

RESUMO

The increasing resistance of plant diseases caused by phytopathogenic fungi highlights the need for highly effective and environmentally benign agents. The antifungal activities of Cnidium monnieri fruit extracts and five isolated compounds as well as structurally related coumarins against five plant pathogenic fungi were evaluated. The acetone extract, which contained the highest amount of five coumarins, showed strongest antifungal activity. Among the coumarin compounds, we found that 4-methoxycoumarin exhibited stronger and broader antifungal activity against five phytopathogenic fungi, and was more potent than osthol. Especially, it could significantly inhibit the growth of Rhizoctonia solani mycelium with an EC 50  value of 21 µg mL -1 . Further studies showed that 4-methoxycoumarin affected the structure and function of peroxisomes, inhibited the ß-oxidation of fatty acids, decreased the production of ATP and acetyl coenzyme A, and then accumulated ROS by damaging MMP and the mitochondrial function to cause the cell death of R. solani  mycelia. 4-Methoxycoumarin presented antifungal efficacy in a concentration- dependent manner in vivo and could be used to prevent the potato black scurf. This study laid the foundation for the future development of 4-methoxycournamin as an alternative and friendly biofungicide.

2.
Zhongguo Dang Dai Er Ke Za Zhi ; 23(8): 814-820, 2021 Aug 15.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-34511171

RESUMO

OBJECTIVES: To study the survival rate and the incidence of complications of very preterm infants and the factors influencing the survival rate and the incidence of complications. METHODS: The medical data of the very preterm infants with a gestational age of <32 weeks and who were admitted to the Department of Neonatology in 11 hospitals of Jiangsu Province in China from January 2018 to December 2019 were retrospectively reviewed. Their survival rate and the incidence of serious complications were analyzed. A multivariate logistic regression analysis was used to evaluate the risk factors for death and serious complications in very preterm infants. RESULTS: A total of 2 339 very preterm infants were enrolled, among whom 2 010 (85.93%) survived and 1 507 (64.43%) survived without serious complications. The groups with a gestational age of 22-25+6 weeks, 26-26+6 weeks, 27-27+6 weeks, 28-28+6 weeks, 29-29+6 weeks, 30-30+6 weeks, and 31-31+6 weeks had a survival rate of 32.5%, 60.6%, 68.0%, 82.9%, 90.1%, 92.3%, and 94.8% respectively. The survival rate tended to increase with the gestational age (P<0.05) and the survival rate without serious complications in each gestational age group was 7.5%, 18.1%, 34.5%, 52.2%, 66.7%, 75.7%, and 81.8% respectively, suggesting that the survival rate without serious complications increased with the gestational age (P<0.05). The multivariate logistic regression analysis showed that high gestational age, high birth weight, and prenatal use of glucocorticoids were protective factors against death in very preterm infants (P<0.05), and 1-minute Apgar score ≤3 was a risk factor for death in very preterm infants (P<0.05); high gestational age and high birth weight were protective factors against serious complications in very preterm infants who survived (P<0.05), while 5-minute Apgar score ≤3 and maternal chorioamnionitis were risk factors for serious complications in very preterm infants who survived (P<0.05). CONCLUSIONS: The survival rate is closely associated with gestational age in very preterm infants. A low 1-minute Apgar score (≤3) may increase the risk of death in very preterm infants, while high gestational age, high birth weight, and prenatal use of glucocorticoids are associated with the reduced risk of death. A low 5-minute Apgar score (≤3) and maternal chorioamnionitis may increase the risk of serious complications in these infants, while high gestational age and high birth weight may reduce the risk of serious complications.


Assuntos
Doenças do Prematuro , Recém-Nascido Prematuro , Feminino , Idade Gestacional , Humanos , Recém-Nascido , Recém-Nascido de muito Baixo Peso , Gravidez , Estudos Retrospectivos , Taxa de Sobrevida
3.
Pestic Biochem Physiol ; 170: 104705, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32980068

RESUMO

Magnolia officinalis, as a well-known herb worldwide, has been widely used to treat multiple diseases for a long time. In this study, the petroleum ether extract from M. officinalis showed effective antifungal activity against seven plant pathogens (particularly against R. solani with an inhibition rate of 100.00% at 250 µg/mL). Honokiol and magnolol, isolated by the bioassay-guided method, exhibited greater antifungal activity than tebuconazole (EC50 = 3.07 µg/mL, p ≤ 0.001) against R. solani, which EC50 values were 2.18 µg/mL and 3.48 µg/mL, respectively. We used transcriptomics to explore the mechanism of action of honokiol against R. solani. Results indicated that honokiol may exert antifungal effects by blocking the oxidative phosphorylation metabolic pathway. Further studies indicated that honokiol induced ROS overproduction, disrupted the mitochondrial function, affected respiration, and blocked the TCA cycle, which eventually inhibited ATP production. Besides, honokiol also damaged cell membranes and caused morphological changes. This study demonstrated that the lignans isolated from M. officinalis possess the potential to be developed as botanical fungicides.


Assuntos
Lignanas/farmacologia , Magnolia , Antifúngicos/farmacologia , Bioensaio , Compostos de Bifenilo
4.
Huan Jing Ke Xue ; 40(9): 4121-4127, 2019 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854876

RESUMO

Upflow anaerobic filter (UAF) with actual domestic wastewater were examined in this study. The Impacts of hydraulic retention time (HRT) on the performance of a UAF and a primary methanogen group were investigated at mesophilic conditions. The chemical oxygen demand (COD) removal rate was more than 75% after 28 days acclimation at 35℃ and HRT of 24 h. With a gradual decrease in the HRT, the COD removal rate first increased and then decreased. When the HRT was 5 h, the COD removal rate was the highest, with an average 81.71% and a maximum of 87.18%. When the HRT decreased to 2.5 h, the average COD removal rate decreased to 75.12%. The methane produced per unit mass of substrate consumed (CH4/CODre) and volume fraction increased with a decrease in HRT. When the HRT was 2.5 h, it reached 0.30 L·g-1, and the volume fraction of methane was maintained at about 73%. The energy generated by the system met the energy demands of the peristaltic pump. Quantitative analysis of the primary methanogen group in the system indicates that Methanosarcinales is the dominant in the system. With a decrease in HRT, the abundances of acetoclastic and hydrogenotrophic methanogens increased significantly.

5.
Oncol Res ; 19(3-4): 171-8, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21473293

RESUMO

This study was performed to determine the risk factors and evaluate the outcome of bilateral breast cancer (BBC). We reviewed the records of 170 patients with BBC and 1,677 with unilateral breast cancer (UBC), and compared their personal history, histopatholgical characteristics, clinical findings, and treatment, and postoperative follow-up records. The patients with UBC were more likely to develop contralateral cancer with the features including: young age at onset, especially younger than 40, premenopause, late primiparity, breast cancer family history, benign mammary disease history, and a tumor larger than 5 cm (p < 0.05). After adjustment by multivariate analysis, we concluded that breast cancer family history and age at onset younger than 40 years old were the independent risk factors for BBC. There were no significant differences for distant metastasis or overall survival between BBC and UBC (p > 0.05). We observed that 64.1% of the second breast cancer occurred within 5 years after the operation of the first cancer, and medical examination could improve the early diagnosis of the contralateral breast cancer. Contrary to common belief, our study showed that BBC and UBC had similar biological features and prognosis (p > 0.05). The excessive treatment and prophylactic measures may be unnecessary in this seemingly aggressive breast cancer. The patients with UBC younger than 40 or with breast cancer family history should have intensive contralateral breast followup, especially within 5 years after in the initial treatment.


Assuntos
Neoplasias da Mama/etiologia , Adulto , Neoplasias da Mama/diagnóstico , Feminino , Lateralidade Funcional , Humanos , Prognóstico , Fatores de Risco
6.
Zhonghua Wai Ke Za Zhi ; 47(24): 1864-7, 2009 Dec 15.
Artigo em Chinês | MEDLINE | ID: mdl-20193403

RESUMO

OBJECTIVE: To study the postoperative complications and its risk factors in patients underwent breast reconstruction with abdominal flaps. METHODS: The clinical data of 115 cases underwent breast reconstructions with abdominal flaps from May 2001 to October 2008 was reviewed. The postoperative complications included total flap necrosis, partial flap necrosis, fat necrosis, hernia, bulge, fat liquefaction and infection. The risk factors of complication rates were also evaluated. RESULTS: The total postoperative complications rate was 17.4% (20/115). No severe complications was found, such as total flap necrosis, hernia and bulge. The most common complications of flap was fat necrosis which occurred in 6 cases (5.2%), partial flap necrosis in 5 cases (4.3%) and infection in 1 case (0.9%). The donor-site complications included fat liquefaction which occurred in 8 cases (7.0%) and infection in 3 cases (2.6%). No significant relation was found between patient's age, body mass index (BMI), timing of surgery and the postoperative complication rate. The postoperative complications occurred more frequently in active smokers, patients with radiotherapy history, or reconstructions with pedicled transverse rectus abdominis myocutaneous (TRAM) flaps. But no significant difference was found in those factors. CONCLUSIONS: Fewer complications happens in patients with a reconstruction with deep inferior epigastric perforator (DIEP) flap. Abdominal flap should be performed with more consideration in active smokers or patients with a radiotherapy history. Age and obesity should not be contraindications to breast reconstruction with abdominal flaps.


Assuntos
Mamoplastia/métodos , Complicações Pós-Operatórias , Retalhos Cirúrgicos , Abdome/cirurgia , Adulto , Neoplasias da Mama/cirurgia , Feminino , Humanos , Mamoplastia/efeitos adversos , Pessoa de Meia-Idade , Complicações Pós-Operatórias/etiologia , Estudos Retrospectivos , Fatores de Risco , Retalhos Cirúrgicos/efeitos adversos
7.
Protein Sci ; 17(4): 664-72, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18359858

RESUMO

The human tissue kallikrein (KLK) family contains 15 secreted serine proteases that are expressed in a wide range of tissues and have been implicated in different physiological functions and disease states. Of these, KLK1 has been shown to be involved in the regulation of multiple physiological processes such as blood pressure, smooth muscle contraction, and vascular cell growth. KLK6 is overexpressed in breast and ovarian cancer tissues and has been shown to cleave peptide derived from human myelin protein and Abeta amyloid peptide in vitro. Here we analyzed the substrate specificity of KLK1 and KLK6, by substrate phage display using a random octapeptide library. Consistent with earlier biochemical data, KLK1 was shown to exhibit both trypsin- and chymotrypsin-like selectivities with Tyr/Arg preferred at site P1, Ser/Arg strongly preferred at P1', and Phe/Leu at P2. KLK6 displayed trypsin-like activity, with the P1 position occupied only by Arg and a strong preference for Ser in P1'. Docking simulations of consensus peptide provide information on the identity of the enzyme residues that are responsible for substrate binding. Bioinformatic analysis suggested several putative KLK6 protein substrates, such as ionotropic glutamate receptor (GluR) and synphilin.


Assuntos
Calicreínas/metabolismo , Calicreínas Teciduais/metabolismo , Sequência de Aminoácidos , Sítios de Ligação , Humanos , Cinética , Modelos Moleculares , Oligopeptídeos/metabolismo , Biblioteca de Peptídeos , Especificidade por Substrato
8.
Zhonghua Zhong Liu Za Zhi ; 30(12): 921-5, 2008 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-19173994

RESUMO

OBJECTIVE: To investigate the factors affecting the long-term survival of patients with carcinoma of esophagus and gastric cardia after curative resection. METHODS: The clinical data of 906 patients with carcinoma of esophagus and gastric cardia treated by radical resection in 1996 - 2004 were analyzed retrospectively. Twelve clinicopathological factors possibly influencing survival were encoded and assessed by Cox regression analysis. RESULTS: The 1-, 3- and 5-year cumulative survival rates were 89.8%, 75.4% and 71.7%, respectively. The univariate analysis showed that age, length of tumor, pathological differentiation, number of metastatic lymph nodes, depth of invasion, involvement of adjacent organs and the TNM stage influenced the prognosis significantly (P < 0.01). However, multivariate analysis showed that pathologic differentiation, number of metastatic lymph nodes, involvement of adjacent organs and TNM stage were independent prognostic factors (P < 0.05). CONCLUSION: The independent prognostic factors of the patients with carcinoma of esophagus and gastric cardia are pathologic differentiation, TNM stage, number of metastatic lymph nodes, and involvement of adjacent organs. The other factors influencing survival are age, length of tumor and depth of invasion. Furthermore, invasion of adjacent organs suggests worse prognosis, and should be followed-up closely.


Assuntos
Carcinoma de Células Pequenas/patologia , Carcinoma de Células Escamosas/patologia , Cárdia , Neoplasias Esofágicas/patologia , Neoplasias Gástricas/patologia , Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Carcinoma de Células Pequenas/cirurgia , Carcinoma de Células Escamosas/cirurgia , Neoplasias Esofágicas/cirurgia , Esofagectomia/métodos , Feminino , Seguimentos , Gastrectomia/métodos , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Neoplasias Gástricas/cirurgia , Taxa de Sobrevida
9.
Zhonghua Yi Xue Za Zhi ; 87(34): 2405-7, 2007 Sep 11.
Artigo em Chinês | MEDLINE | ID: mdl-18036318

RESUMO

OBJECTIVE: To investigate the trend of clinical characteristics and prognosis of breast cancer in women during the past 2 decades (1981 - 2000). METHODS: 1678 cases were sampled randomly from the 11400 breast cancer patients who were hospitalized in Tianjin Medical University Cancer Hospital 1981 - 2000. Retrospective analysis of the clinical characteristics and prognosis between different eras was conducted. RESULTS: The numbers of hospitalized female breast cancer patients during the periods 1981 - 1985, 1986 - 1990, 1991 - 1995, 1996 - 2000 were 209, 316, 449, and 704 respectively with an increase of 237% within 20 years and an annual increase rate of 11.8%. The mean onset age remained 46 - 47. The detection rate of breast cancer at early stages (stages 0 approximately I) was 15.5% during 1996 - 2000, higher than that during 1981 - 1985 (12%), and the detection rate of breast cancer at stage III during 1996 - 2000 was 16.5%, lower than that during 1981 - 1985 (24.9%). The proportion of carcinoma in situ during 1996 - 2000 was 4.4%, higher than that during 1981 - 1985 (3.7%). The axillary lymph node positive rate during 1996 - 2000 was 46.3%, lower than that during 1981 - 1985 (51.8%s), and the local recurrence/distance metastasis rate during 1996 - 200 was 13.5%, lower than that during 1981 - 1985 (27.8%). The 5-year and 10-year survival rates during 1996 - 2000 were 86% and 74%, both higher than those during 1981 - 1985 (76% and 63% respectively). CONCLUSION: During the past 2 decades, the incidence of breast cancer shows an uptrend. The proportion of early stage breast cancer increases gradually and the proportion of axillary lymph node positive rate and metastasis rate show a downtrend. The prognosis of the breast cancer patients has been improved during these periods.


Assuntos
Neoplasias da Mama/epidemiologia , Neoplasias da Mama/patologia , Adulto , Idoso , China/epidemiologia , Feminino , Seguimentos , Hospitalização/estatística & dados numéricos , Hospitalização/tendências , Humanos , Metástase Linfática , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos , Análise de Sobrevida
10.
Zhonghua Liu Xing Bing Xue Za Zhi ; 26(9): 716-9, 2005 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-16471226

RESUMO

OBJECTIVE: To describe survival conditions of patients with stroke and to analyze the factors associated with survival, a seven-year follow-up study was carried out. METHODS: From Nov. 1995 to Dec. 1996, 189 stroke patients were selected in the Department of Neurology, General Hospital, Tianjin Medical University. Cases were followed up since the onset of stroke. Data collected would include case history, illness and survival conditions. Kaplan-Meier methods were used for survival description. Cox regression was used for prognostic factors analyses. RESULTS: A total number of 82 patients had been dead during the period of study and among them,58 cases died from stroke. The survival rate was 79.86% in one year, 65.46% in three years and 57.46% in seven years. Factors with statistical significance that associated with survival would include: age (RR = 1.065, P < 0.001), physical exercises before stroke (RR = 0.308, P<0.001), hypertension history (RR = 1.785, P < 0.05) and stroke history (RR =2.493, P < 0.001) while factors associated with severity of the illness were: area of cerebral lesion, conditions when discharged from the hospital, rehabilitative treatment of post-discharge and recurrence. We also found that social-psychical factors as rehabilitative confidence, repression, negative event, support from relatives and friends were related to survival of stroke. CONCLUSIONS: Histories on hypertension, stroke and brain injury condition were related to the rate survival on stroke. Patients persisting physical exercises before stroke had better prognosis. The survival rate of patients with recurrence was lower than those without while social psychic factors might be related to survival.


Assuntos
Acidente Vascular Cerebral/diagnóstico , Feminino , Seguimentos , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Qualidade de Vida , Análise de Sobrevida
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