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1.
J Colloid Interface Sci ; 629(Pt A): 409-421, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36087555

RESUMO

Methylene blue (MB) is a very widely used cationic dye for color cotton and silk products. The harmful MB has a complex aromatic structure that is difficult to be degraded in natural aqueous. In this study for the first time, a novel double Z-type ternary composite long-afterglow/graphitic carbon nitride@metal-organic framework (SrAl2O4:Eu2+,Dy3+/g-C3N4@NH2-UiO-66, SGN) has been synthesized by solvothermal method, and was used as a new photocatalyst for removal of MB. The photocatalytic performance of the composited photocatalyst SGN was largely improved compared to that of the non-composite. After 30 min of photocatalysis, the degradation ratio of SGN was 5.86, 4.04 and 10 times more efficient than that of long-afterglow (SAO), g-C3N4 and NH2-UiO-66, respectively. The high photocatalytic activity of the SGN could be ascribed to the double Z-type electron transfer mechanism. More importantly, due to the luminescence of SAO, the degradation ratio of ternary SGN can reach about 50 % after 5 h in the dark. Superoxide anion (·O2-) was verified to be the main active substance in the photocatalytic process by quenching experiments and electron spin resonance (ESR) spectra analysis. The total organic matter contents (TOC) and partial degradation products were measured, which confirmed that some MB was mineralized into H2O and CO2, and some was degraded into smaller molecules. Moreover, the excellent stability and recyclability of this catalyst were also investigated. Here, a new promising material with high degradation performance for water pollution treatment is presented.


Assuntos
Estruturas Metalorgânicas , Poluentes Químicos da Água , Azul de Metileno/química , Superóxidos , Dióxido de Carbono , Poluentes Químicos da Água/química , Seda
2.
Int J Clin Health Psychol ; 23(1): 100343, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36299492

RESUMO

Background/Objective: Self-compassion has a consensual relevance for overall mental health, but its mechanisms remain unknown. Using intermittent theta burst stimulation (iTBS) and concurrent transcranial magnetic stimulation-electroencephalography (TMS-EEG), this study investigated the causal relationship of the dorsolateral prefrontal cortex (DLPFC) with self-compassion and explored the changes in neuroplasticity and neural dynamics. Method: Thirty-two healthy participants received iTBS or sham stimulation over the DLPFC, before and after which they were instructed to either use self-compassionate strategies or to be rejected in the context of social rejection and to report the level of self-compassion or negative affect. TMS-evoked potentials were evaluated as novel neuroplastic techniques with N45, P60, N100, and P180. Results: iTBS uniquely decreased P180 amplitude measured with TMS-EEG whereby sham stimulation had no effect on neuroplasticity. In line with neuroplasticity changes, iTBS enhanced a widespread gamma band power and coherence, which correlated consistently with increased engagement in self-compassion. Meanwhile, iTBS demonstrated opposite effects on theta activity dependent on the social contexts whereby self-compassion decreased and social rejection enhanced it respectively. This unique effect of iTBS on theta activity was also supplemented by the enhancement of theta band coherence following iTBS. Conclusions: We found a causal relationship between DLPFC and self-compassion. We also provide evidence to indicate widespread gamma activity and connectivity to correlate with self-compassion as well as the critical role of the DLPFC in modulating theta activity and negative emotions.

3.
J Hazard Mater ; 441: 129919, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36099738

RESUMO

Methylmercury (MeHg+) is one of the common organic species of mercury, and has much higher toxicity than inorganic mercury. Based on the selective enhancement of the activity of nanozyme (NA-CDs/AuNPs) by MeHg+, a novel colorimetric nanoprobe for MeHg+ assay is proposed. The noradrenaline-based carbon dots (NA-CDs) as the reducing agent was applied to prepare the NA-CDs/AuNPs. The formation of gold amalgamation (Au@HgNPs) between nanozyme and MeHg+ allows to simultaneously accelerate the electron transfer from Au and Hg to NA-CDs and the generation of radicals (i.e. ∙OH, ∙O2- and ∙CH3). The NA-CDs/AuNPs has an outstanding anti-interference performance even in the presence of different mercury. Further density functionality theory (DFT) calculations revealed that the formation of Au@HgNPs via MeHg+ contributes to the significantly lowered activation energy, resulting in the peroxidase-like activity generation and acceleration. This leads to rapid (10 min) and specific colorimetric detection of MeHg+ with the detection limit of 0.06 µg L-1. This introduces a novel method for simple and sensitive detection of MeHg+, giving a new horizon for the assay of organometallic compounds.


Assuntos
Mercúrio , Nanopartículas Metálicas , Compostos de Metilmercúrio , Carbono , Colorimetria/métodos , Ouro , Norepinefrina , Peroxidases , Substâncias Redutoras
4.
Carbohydr Polym ; 301(Pt A): 120299, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36436865

RESUMO

We develop a kind of photocurable liquid crystal hydrogels with bone extracellular matrix (ECM)-like liquid crystal state and viscoelasticity, as well as different chargeability. First, positively charged chitin whiskers (CHWs) and negatively charged maleic anhydride chitin whiskers (mCHWs) were prepared, which further self-assemble to form chiral nematic liquid crystals under ultrasonic treatment, respectively. Subsequently, poly (ethylene glycol) diacrylate (PEGDA) and photo initiator were added, and then two kinds of liquid crystal hydrogels with bone ECM-like viscoelasticity and different chargeability were prepared under ultraviolet (UV) irradiation. Benefiting from the bone ECM-like liquid crystal state and viscoelasticity, the prepared liquid crystal hydrogels exhibit remarkable cell affinity and osteogenic ability. Moreover, the liquid crystal hydrogel with negatively charged mCHWs is more favorable for cell adhesion, spreading and osteogenic differentiation than that with positively charged CHWs. This work provides a promising strategy to prepare the hydrogels with bone ECM-like liquid crystal properties and viscoelasticity for bone repair.


Assuntos
Hidrogéis , Cristais Líquidos , Animais , Hidrogéis/química , Quitina/química , Osteogênese , Vibrissas
5.
Artigo em Inglês | MEDLINE | ID: mdl-36181957

RESUMO

Humans gain knowledge about threats not only from their own experiences but also from observing others' behavior. A neutral stimulus is associated with a threat stimulus for several times and the neutral stimulus will evoke fear responses, which is known as fear conditioning. When encountering a new event that is similar to one previously associated with a threat, one may feel afraid and produce fear responses. This is called fear generalization. Previous studies have mostly focused on fear conditioning and generalization based on direct learning, but few have explored how observational fear learning affects fear conditioning and generalization. To the best of our knowledge, no previous study has focused on the neural correlations of fear conditioning and generalization based on observational learning. In the present study, 58 participants performed a differential conditioning paradigm in which they learned the associations between neutral cues (i.e., geometric figures) and threat stimuli (i.e., electric shock). The learning occurred on their own (i.e., direct learning) and by observing other participant's responses (i.e., observational learning); the study used a within-subjects design. After each learning condition, a fear generalization paradigm was conducted by each participant independently while their behavioral responses (i.e., expectation of a shock) and electroencephalography (EEG) recordings or responses were recorded. The shock expectancy ratings showed that observational learning, compared to direct learning, reduced the differentiation between the conditioned threatening stimuli and safety stimuli and the increased shock expectancy to the generalization stimuli. The EEG indicated that in fear learning, threatening conditioned stimuli in observational and direct learning increased early discrimination (P1) and late motivated attention (late positive potential [LPP]), compared with safety conditioned stimuli. In fear generalization, early discrimination, late motivated attention, and orienting attention (alpha-event-related desynchronization [alpha-ERD]) to generalization stimuli were reduced in the observational learning condition. These findings suggest that compared to direct learning, observational learning reduces differential fear learning and increases the generalization of fear, and this might be associated with reduced discrimination and attentional function related to generalization stimuli.


Assuntos
Medo , Generalização Psicológica , Humanos , Medo/fisiologia , Generalização Psicológica/fisiologia , Condicionamento Clássico/fisiologia , Aprendizagem/fisiologia , Atenção
6.
J Ethnopharmacol ; 302(Pt A): 115761, 2023 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-36309113

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Houttuynia cordata Thunb. (HC, Saururaceae family) is a classical Traditional Chinese Medicine used to treat pneumonia clinically. The total flavonoids (HCF) and polysaccharides (HCP) are key medicinal components of H. cordata involved in its beneficial effect on viral pneumonia. AIM OF THE STUDY: The purpose of the study is to investigate the synergistic or complementary effects of combination of HCF and HCP on viral pneumonia as well as the mechanisms underlying. MATERIALS AND METHODS: HCF or HCP were administrated separately or combined in different proportions on influenza virus H1N1 - infected mice. The survival and lung weight of mice were recorded. The synergistic effect on HCF and HCP combination was calculated by Chou-Talalay method. H&E staining was performed to detect lung histomorphology. Western blot, immunohistochemistry and enzyme linked immunosorbent assay were done to analyze the representative protein expression in lung and intestine tissues. AB - PAS staining on intestine tissue sections was performed to evaluate the histopathology of intestines. Bacterial genomic DNA was extracted and sequenced for gut microbiota analysis. RESULTS: In H1N1 lethally infected mice, the combined administration of HCF and HCP significantly increased the survival rate and prolonged the life span of mice, compared with mono-drug therapy. The viral pneumonia was remarkably improved by HCF and HCP combination reflected by lower lung index, more intact lung morphology, and less inflammatory cells and mediators. Furthermore, the combination of HCF and HCP regulated intestinal microbiota, significantly reduced the proportion of pathogenic Proteobacteria and the secretion of proinflammatory cytokine in gut. The combined HCF and HCP showed synergistic effect on reducing lung and intestine injury. The complementary interaction was also found in HCF and HCP combined therapy, as HCF provided the significant antiviral activity and HCP markedly improved intestinal physical barrier and increased the protein expression involving removal of edema. CONCLUSIONS: Our findings indicated that combination of HCF and HCP from H. cordata synergistically alleviated H1N1-induced viral pneumonia in mice via multimodal regulation of both pulmonary and intestinal homeostasis, which might imply novel therapeutic strategy for treating viral pneumonia.


Assuntos
Houttuynia , Vírus da Influenza A Subtipo H1N1 , Pneumonia Viral , Camundongos , Animais , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Polissacarídeos/farmacologia , Polissacarídeos/uso terapêutico , Pneumonia Viral/tratamento farmacológico
7.
J Ethnopharmacol ; 302(Pt B): 115917, 2023 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-36414215

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Huanglian Jiedu plaster (HJP) is a kind of Chinese patent medicine that contains four medicinal plants. It has been clinically proven to be beneficial for the treatment of tumor-associated radiation dermatitis. However, the underlying mechanism of HJP on radiation dermatitis remains unclear. AIM OF THE STUDY: This study aims to investigate the therapeutic effect of HJP on X-ray-induced radiation dermatitis, and how HJP improves the inflammatory response and skin damage of radiation dermatitis. MATERIALS AND METHODS: In this study, We selected a case of esophageal cancer as a clinical demonstration of the efficacy of radiation dermatitis. The patient received a total radiation dose of 7000cGY, and treatment by HJP for 14 days.RD mouse models were established through continuous irradiation with X-ray (800cGY) on the right hind limb of mice for 5 days, and the treatment group mice was applied HJP to the irradiated skin for 15 days from modeling. An inflammatory cellular model was induced through irradiation with X-ray (100cGY) in JB6 cells and a co-culture system of JB6 cell and macrophage was established to examine the effect and mechanism of HJP on the inflammatory interaction of these two cells. The activation of HMGB1-TLR4-NF-κB signaling pathway, and the levels of epidermal injury related factors and inflammatory cytokins were subsequently detected. RESULTS: The results showed that HJP can significantly alleviate X-ray-induced skin injury, inhibiting skin inflammation and reducing the expression of inflammatory cytokins (IL-1ß, IL-6, TNF-α) and epidermal damage related factors (Integrin ß1, CXCL9 and Cytokeratin17), as well as significantly down-regulated the protein level of HMGB1 (a key DAMPs factor) in vivo and in vitro. Cell co-culture experiments demonstrated that HMGB1 released from X-ray-induced JB6 cells can promote inflammatory response of macrophage, which then feedback aggravate epithelial cell damage, notably, HJP can significantly improve radiation skin lesion by inhibiting HMGB1-mediated inflammatory interaction between epithelial cells and macrophages. CONCLUSION: In summary, these findings indicated the role of HJP in the treatment of RD by inhibiting the inflammatory interaction between macrophage and JB6 cells mediated by HMGB1, which may provide a reliable therapeutic method for RD. Furthermore, HMGB1 may be an effective target for HJP to inhibit inflammation and ameliorate skin damage in RD.


Assuntos
Dermatite , Proteína HMGB1 , Camundongos , Animais , Raios X , Macrófagos , Inflamação
8.
J Hazard Mater ; 444(Pt A): 130416, 2023 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-36403455

RESUMO

Arsenic (As) mobilisation assists in remediating As-contaminated soils but might increase ecological and health risks. In this study, risks of applying two mobilising agents were assessed, i.e. an emerging reducing-chelating composite agent [dithionite (Na2S2O4)-EDTA] and a classical low-molecular-weight organic acid (LMWOA) [citric acid (C6H8O7)]. Results showed that both agents induced sharp increase in leachability-based ecological risk of As. Interestingly, the two agents had opposite performances regarding health risks. Na2S2O4-EDTA significantly increased As relative bioavailability (RBA) to 1.83 times that in controls based on in vivo mouse model, and As bioaccessibility to 1.96, 1.65 and 1.20 times in gastric, small intestinal and colon phases based on in vitro PBET-SHIME model. Besides, it caused significant increase of highly toxic As(Ⅲ) in colon fluid. In contrast, C6H8O7 significantly reduced RBA and bioaccessibility of soil As in colon by 44.44% and 14.65%, respectively. Importantly, C6H8O7 restrained bioaccessible As(V) reduction and promoted bioaccessible As(Ⅲ) methylation, further reducing health risk. The phenomena could mainly be attributed to excessive metal components release from soil by C6H8O7 and gut microbiota metabolism of C6H8O7. In summary, C6H8O7 and similar LMWOAs are recommended. The study contributes to mobilising agent selection and development and provides a reference for managing remediation sites.


Assuntos
Arsênio , Animais , Camundongos , Ditionita , Arsênio/toxicidade , Disponibilidade Biológica , Ácido Cítrico , Ácido Edético , Solo
9.
Sci Total Environ ; 858(Pt 2): 159957, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36343820

RESUMO

The consumption of similar diets has led to the convergence of gut microbial compositions and functions across phylogenetically distinct animals. However, given the functional redundancy in gut microbiomes, it remains unclear whether synchrony occurs in their functions only and not in their composition, even within phylogenetically close animals consuming a similar diet. In this study, we collected fresh fecal samples from a Rhinopithecus roxellana population in April 2021 (before food provisioning) and June and December 2021 (after food provisioning) and used high-throughput sequencing methods (full-length 16S rRNA gene sequencing and metagenomes) to investigate changes in the gut microbiome due to food provisioning. Combining the results from our previous studies on a wild Rhinopithecus bieti population, we found that the artificial food provisions (e.g., apples, carrots, and peanuts) affected the gut microbiome, and synchrony occurred only in its functions and antibiotic resistance gene community in both Rhinopithecus species, reflecting its ecological functional redundancy. Given the current findings (e.g., depletion in probiotic microbes, dysbiosis in the gut microbial community, and changes in the antibiotic resistance gene profile), anthropogenic disturbances (e.g., food provisioning) would have potential negative effects on host health. Therefore, human activity in animal conservation should be rethought from the standpoint of gut microbial diversity.


Assuntos
Microbioma Gastrointestinal , Presbytini , Humanos , Animais , Microbioma Gastrointestinal/genética , RNA Ribossômico 16S/genética , Bactérias , Fezes
10.
Chemosphere ; 312(Pt 1): 137293, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36403811

RESUMO

Standard reference materials (SRMs) have been commonly used to perform quality assurance and quality control (QA/QC) in soil total metal concentration analyses or bioaccessibility assessment. In this study, 10 experimenters from 4 laboratories determined bioaccessibility of lead (Pb) in 4 widely-used SRMs (NIST 2710a, NIST 2587, BGS 102, and GBW 07405). Based on the gastric phase (GP) of the unified BARGE bioaccessibility method (UBM) and the Solubility Bioavailability Research Consortium procedure (SBRC), Pb bioaccessibility in SRMs was compared within and between laboratories to assess their intra-laboratory repeatability and inter-laboratory reproducibility. Lead bioaccessibility was 14.1 ± 2.44%-101 ± 2.48% in the 4 SRMs. The values were in vivo validated based on a mouse model in previous studies (R2 = 0.97-0.98), suggesting the reliability of Pb bioaccessibility data. Strong correlations were observed for Pb bioaccessibility among 7 experimenters (R2 = 0.94-0.99) at the Nanjing University (NJU) laboratory and similar strong correlations were also found between each two of the 4 laboratories (R2 = 0.94-0.98), illustrating consistency in intra- and inter-laboratory performance. The intra-laboratory repeatability and inter-laboratory reproducibility were generally acceptable with relative standard deviations (RSDs) of Pb bioaccessibility being ≤10% within laboratory and ≤20% between laboratories, except in a soil with low bioaccessible Pb (BSG 102). Our study suggested that measurements of Pb bioaccessibility in SRMs based on the two in vivo validated methods were repeatable and reproducible within and between laboratories, further verified their reliability being used as QA/QC samples during Pb bioaccessibility assessment.

11.
Int J Hyg Environ Health ; 247: 114076, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36427387

RESUMO

Air pollutants can cause serious harm to human health and a variety of respiratory diseases. This study aimed to explore the associations between air pollutants and outpatient visits for influenza-like illness (ILI) under urbanization process in the sub-center of Beijing. The data of ILI in sub-center of Beijing from April 1, 2014 to December 31, 2020 were obtained from Beijing Influenza Surveillance Network. A generalized additive Poisson model was applied to examine the associations between the concentrations of air pollutants and daily outpatient visits for ILI when controlling meteorological factors and holidays. A total of 322,559 patients with ILI were included. The results showed that in the early urbanization period, the effects of PM2.5, PM10, SO2, O3, and CO on lag0 day, and PM2.5, PM10, O3, and CO on lag1 day were not significant. In the later urbanization period, AQI and the concentrations of PM2.5, PM10, SO2, NO2 and CO on lag1 day were all significantly associated with an increased risk of outpatient visits for ILI, which increased by 0.34% (95%CI 0.23%, 0.45%), 0.42% (95%CI 0.29%, 0.56%), 0.44% (95%CI 0.33%, 0.55%), 0.36% (95%CI 0.24%, 0.49%), 0.91% (95%CI 0.62%, 1.21%) and 0.38% (95%CI 0.26%, 0.49%). The concentration of O3 on lag1 day was significantly associated with a decreased risk of outpatient visits for ILI, which decreased by 0.21% (95%CI 0.04%, 0.39%). We found that the urbanization process had significantly aggravated the impact of air pollutants on ILI outpatient visits. These findings expand the current knowledge of ILI outpatient visits correlated with air pollutants under urbanization process.


Assuntos
Poluentes Atmosféricos , Influenza Humana , Viroses , Humanos , Urbanização , Pequim/epidemiologia , Influenza Humana/epidemiologia , Pacientes Ambulatoriais , Material Particulado
12.
Biomed Chromatogr ; : e5557, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36453605

RESUMO

Based on the biolabel research pattern, omics and network pharmacology were used for exploring the neuroprotection of Sophora tonkinensis (ST) in the treatment of brain diseases. Multi-omics were applied to investigate biolabels for ST intervention in brain tissue. Based on biolabels, the therapeutic potential, mechanism, and material basis of ST treating brain diseases were topologically analyzed by network pharmacology. A Parkinson's disease (PD) mouse model was used to validate biolabel analysis results. Four proteins and three metabolites were involved in two key pathways (alanine, aspartate, and glutamate metabolism and arginine biosynthesis) and considered as biolabels. Network pharmacology showed that ST had the potential to treat some brain diseases, especially PD. Eight compounds (caffeic acid, gallic acid, cinnamic acid, etc.) may serve as the material basis of ST treating brain diseases via the mediation of three biolabels. In the PD model, ST and its active compounds (caffeic acid and gallic acid) may protect dopaminergic neurons (Maximum recovery rate for dopamine: 49.5%) from oxidative stress (PRKN, ROS, NO, etc.) and neuroexcitatory toxicity (GD, glutamine, glutamic acid, etc.). These findings indicated that omics and network pharmacology may contribute to the achievement of the objectives of this study based on the biolabel research pattern.

13.
Am J Med Genet A ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36454652

RESUMO

Monoallelic variants of CTCF cause an autosomal dominant neurodevelopmental disorder with a wide range of features, including impacts on the brain, growth, and craniofacial development. A growing number of subjects with CTCF-related disorder (CRD) have been identified due to the increased application of exome sequencing, and further delineation of the clinical spectrum of CRD is needed. Here, we examined the clinical features, including facial profiles, and genotypic spectrum of 107 subjects with identified CTCF variants, including 43 new and 64 previously described subjects. Among the 43 new subjects, 23 novel variants were reported. The cardinal clinical features in subjects with CRD included intellectual disability/developmental delay (91%) with speech delay (65%), motor delay (53%), feeding difficulties/failure to thrive (66%), ocular abnormalities (56%), musculoskeletal anomalies (53%), and behavioral problems (52%). Other congenital anomalies were also reported, but none of them were common. Our findings expanded the genotypic and phenotypic spectrum of CRD that will guide genetic counseling, management, and surveillance care for CRD patients with CRD. Additionally, a newly built facial gestalt on the Face2Gene tool will facilitate prompt recognition of CRD by physicians and shorten a patient's diagnostic odyssey.

14.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 39(12): 1370-1374, 2022 Dec 10.
Artigo em Chinês | MEDLINE | ID: mdl-36453961

RESUMO

OBJECTIVE: To analyze the genotype and clinical phenotype of a 3-month-old female infant featuring unresponsiveness. METHODS: The infant was subjected to genetic testing, and her clinical features were compared with syndromes associated with variants of the candidate gene. RESULTS: The patient has featured long fingers, long and overlapped toes, musk-like face, blepharophimosis, ptosis, and lacrimal duct anomaly. She was found to harbor a heterozygous de novo variant NM_012330.3: c.3040C>T (p.Gln1014*) in exon 16 of the KAT6B gene. Her clinical phenotype and genotype have both conformed to Say-Barber-Biesecker-Young-Simpson syndrome (SBBYSS). CONCLUSION: The child was diagnosed with SBBYSS syndrome due to the c.3040C>T (p.Gln1014*) variant of the the KAT6B gene. Discovery of the unique features has expanded the phenotypic spectrum of this syndrome.


Assuntos
Blefarofimose , Blefaroptose , Feminino , Humanos , Blefarofimose/diagnóstico , Blefarofimose/genética , Família , Genótipo , Síndrome , Histona Acetiltransferases
15.
Brief Bioinform ; 2022 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-36464489

RESUMO

Viruses are the most ubiquitous and diverse entities in the biome. Due to the rapid growth of newly identified viruses, there is an urgent need for accurate and comprehensive virus classification, particularly for novel viruses. Here, we present PhaGCN2, which can rapidly classify the taxonomy of viral sequences at the family level and supports the visualization of the associations of all families. We evaluate the performance of PhaGCN2 and compare it with the state-of-the-art virus classification tools, such as vConTACT2, CAT and VPF-Class, using the widely accepted metrics. The results show that PhaGCN2 largely improves the precision and recall of virus classification, increases the number of classifiable virus sequences in the Global Ocean Virome dataset (v2.0) by four times and classifies more than 90% of the Gut Phage Database. PhaGCN2 makes it possible to conduct high-throughput and automatic expansion of the database of the International Committee on Taxonomy of Viruses. The source code is freely available at https://github.com/KennthShang/PhaGCN2.0.

16.
ACS Nano ; 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36469414

RESUMO

Implanting a 3D printing scaffold is an effective therapeutic strategy for personalized bone repair. As the key factor for the success of bone tissue engineering, the scaffold should provide an appropriate bone regeneration microenvironment and excellent mechanical properties. In fact, the most ideal osteogenic microenvironment is undoubtedly provided by natural bone extracellular matrix (ECM), which exhibits liquid crystalline and viscoelastic characteristics. However, mimicking a bone ECM-like microenvironment in a 3D structure with outstanding mechanical properties is a huge challenge. Herein, we develop a facile approach to fabricate a bionic scaffold perfectly combining bone ECM-like microenvironment and robust mechanical properties. Creatively, 3D printing a poly(l-lactide) (PLLA) scaffold was effectively strengthened via layer-by-layer electrostatic self-assembly of chitin whiskers. More importantly, a kind of chitin whisker/chitosan composite hydrogel with bone ECM-like liquid crystalline state and viscoelasticity was infused into the robust PLLA scaffold to build the bone ECM-like microenvironment in 3D structure, thus highly promoting bone regeneration. Moreover, deferoxamine, an angiogenic factor, was encapsulated in the composite hydrogel and sustainably released, playing a long-term role in angiogenesis and thereby further promoting osteogenesis. This scaffold with bone ECM-like microenvironment and excellent mechanical properties can be considered as an effective implantation for bone repair.

17.
Artigo em Inglês | MEDLINE | ID: mdl-36454561

RESUMO

Importance: Although 1 in 4 head and neck cancer (HNC) survivors experience clinically significant body image distress (BID), a psychosocial morbidity that adversely affects quality of life, effective interventions for these patients are lacking. Objective: To evaluate the acceptability and preliminary efficacy of BRIGHT (Building a Renewed ImaGe after Head and neck cancer Treatment), a brief tele-cognitive behavioral therapy, at reducing BID among HNC survivors. Design, Setting, and Participants: This parallel-group pilot randomized clinical trial recruited adult HNC survivors with BID between August 13, 2020, and December 9, 2021, from the Medical University of South Carolina HNC clinic during a routine survivorship encounter. Data were analyzed from May 3 to June 16, 2022. Interventions: BRIGHT consisted of 5 weekly psychologist-led video tele-cognitive behavioral therapy sessions. Attention control (AC) consisted of dose- and delivery-matched survivorship education. Main Outcomes and Measures: Change in HNC-related BID was assessed using IMAGE-HN (Inventory to Measure and Assess imaGe disturbancE-Head and Neck), a validated patient-reported outcome (score range, 0-84, with higher scores indicating greater HNC-related BID). Clinical response rate was measured as the proportion of patients with a clinically meaningful change in IMAGE-HN scores. Results: Of the 44 HNC survivors with BID allocated to BRIGHT (n = 20) or AC (n = 24), the median (range) age was 63 (41-80) years, and 27 patients (61%) were female. Patients rated BRIGHT's acceptability highly (all metrics had a mean rating of ≥4.5/5), and 19 of 20 patients (95%) receiving BRIGHT were likely or highly likely to recommend it to other HNC survivors with BID. BRIGHT decreased HNC-related BID from baseline to 1 month postintervention relative to AC (mean model-based difference in change in IMAGE-HN score, -7.9 points; 90% CI, -15.9 to 0.0 points) and from baseline to 3 months postintervention relative to AC (mean model-based difference in change in IMAGE-HN score, -17.1 points; 90% CI, -25.6 to -8.6 points). At 3 months postintervention, the clinical response rate of BRIGHT was 6.6-fold higher than AC (model-based odds ratio, 6.6; 90% CI, 2.0-21.8). The improvement in HNC-related BID for BRIGHT vs AC at 3 months was clinically significant, and the effect size was large (Cohen d, -0.9; 90% CI, -1.4 to -0.4). Conclusions and Relevance: In this pilot randomized clinical trial, BRIGHT was acceptable, may result in a clinically meaningful improvement in HNC-related BID, and showed a high clinical response rate. These promising preliminary data support conducting a large efficacy trial to establish BRIGHT as the first evidence-based treatment for HNC survivors with BID. Trial Registration: ClinicalTrials.gov Identifier: NCT03831100.

18.
Front Oncol ; 12: 917366, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36457496

RESUMO

Objective: The overexpression of polo-like kinase 1 (PLK-1) has been found in a broad spectrum of human tumors, making it an attractive prognostic tumor biomarker. Nowadays, PLK-1 is considered a cancer therapeutic target with clinical therapeutic value. The aim of the present study was to systematically review the prognostic and therapeutic value of PLK-1 in different malignant neoplasms. Methods: A systematic literature search of the Cochrane Library, PubMed, Web of Science, and China National Knowledge Internet (CNKI) databases was conducted between December 2018 and September 2022. In total, 41 published studies were screened, comprising 5,301 patients. We calculated the pooled odds ratios (ORs) and corresponding 95%CIs for the clinical parameters of patients included in these studies, as well as the pooled hazard ratios (HRs) and corresponding 95% CIs for 5-year overall survival (OS). Results: Our analysis included 41 eligible studies, representing a total of 5,301 patients. The results showed that overexpression of PLK-1 was significantly associated with poor OS (HR, 1.57; 95% CI, 1.18-2.08) and inferior 5-year disease-free survival/relapse-free survival ((HR, 1.89; 95% CI, 1.47-2.44). The pooled analysis showed that PLK-1 overexpression was significantly associated with lymph node metastasis, histological grade, clinical stages (p < 0.001 respectively), and tumor grade (p < 0.001). In digestive system neoplasms, PLK-1 overexpression was significantly associated with histopathological classification, primary tumor grade, histological grade, and clinical stages (p = 0.002, p = 0.001, p < 0.0001, respectively). In breast cancer, PLK-1 was significantly associated with 5-year overall survival, histological grade, and lymph node metastasis (p < 0.001, p = 0.003, p < 0.001, respectively). In the female reproductive system, PLK-1 was significantly associated with clinical stage (p = 0.011). In the respiratory system, PLK-1 was significantly associated with clinical stage (p = 0.021). Conclusion: Our analysis indicates that high PLK-1 expression is associated with aggressiveness and poor prognosis in malignant neoplasms. Therefore, PLK-1 may be a clinically valuable target for cancer treatment.

19.
J Exp Bot ; 2022 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-36462194

RESUMO

Most orchids have high ornamental value with long-lived flower. However, the mechanisms by which orchids maintain floral longevity are poorly understood. Here, we hypothesized that long floral longevity in Dendrobium is maintained by high resource investment and complementary water and nutrient utilization in different structural units of the perianth. To test this hypothesis, we determined which water- and nutrient-related traits are correlated with flower longevity in twenty-three Dendrobium species or cultivars and examined the variations of the related traits during flower development of one long-lived cultivar. We found that floral longevity was correlated with dry mas per unit area of perianths and total flower biomass, which indicates that maintaining long floral longevity requires increased resource investment. During development of long-lived flowers, labella showed high capacity for water storage and nutrient reutilization, which can partly remedy high water demand and biomass investment. Sepals and petals, in contrast, had stronger desiccation avoidance and higher metabolic activity with lower biomass investment. These findings indicate that Dendrobium flowers maintain longevity by complementary water and utilization strategies in the sepals, petals and labella, with labella consuming more water and nutrients to extend flower display, while sepals and petals use a more conservative strategy.

20.
Front Public Health ; 10: 1040410, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466472

RESUMO

Aim: Public concerns over the mental health problems of college students are rising. Previous research show that female tend to suffer more from mental health problems than males, with few studies focusing on males. This study sought to explore the association of lifestyle-related risk factors with the prevalence of mental health problems among male college students in China. Methods: The lifestyle information and mental health status of 686 male college students from Chongqing, China, were assessed in 2014, and 582 of them were followed up a year later. Participants completed a questionnaire assessing demographic and lifestyle factors which include sleep quality, computer usage, sedentariness, physical activity, smoking, current alcohol, coke, coffee, and milk tea drinking, and current tea/fried food/baked food consumption. Mental health problems were measured using the Depression Anxiety Stress Scale-21 (DASS-21). Results: Univariate analyses indicated that age, sleep latency, sleep duration, computer usage time, milk tea drinking, and fried food consumption were potential risk factors for mental health problems (p's < 0.05). Multivariate analysis further revealed that, either at baseline or during follow-up, participants with (i) more computer usage time were at a higher risk of having depression symptoms (p's < 0.05) and (ii) a higher frequency of fried food consumption were associated with a higher risk of having depression, anxiety, and stress symptoms (p's < 0.05). Additionally, the cross-lagged analysis showed that (i) computer usage time in 2014 is positively correlated with depression status (ß = 0.106, p < 0.05) but not anxiety (ß = 0.047, p > 0.05) and stress (ß = 0.019, p > 0.05) status a year later and (ii) fried food consumption in 2014 is positively correlated with depression (ß = 0.129, p < 0.01), anxiety (ß = 0.168, p < 0.001), and stress (ß = 0.113, p < 0.01) status a year later. Conclusions: Computer usage time and fried food consumption were lifestyle-related risk factors for mental health problems in male college students in Chongqing, China. These results might emphasize further preventive strategies for mental health problems, especially in male college students.


Assuntos
Saúde Mental , Estudantes , Humanos , Feminino , Masculino , Estilo de Vida , Fatores de Risco , China/epidemiologia
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