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1.
Anal Chem ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32048503

RESUMO

Selectivity is a crucial parameter for photoelectrochemical (PEC) sensing in a practical setting. Despite the use of specific probes such as aptamers, antibodies, and enzymes, coexisting interferences can still result in inaccuracies in PEC sensing, especially for complex biosample matrices. Here we report the design of a Fe3O4@SiO2@TiO2 magnetic-optical bifunctional beacon applied in a novel PEC sensor that can selectively capture progesterone in complex bio-samples, be magnetically separated and cleaned, and be detected in pure phosphate buffer solution (PBS). The magnetic separation strategy efficiently removes the complex coexisting species from the modified electrode surface and drastically enhances the selectivity of the as-designed PEC sensor. The as-designed PEC sensor is cost-effective, easy to fabricate, highly selective and sensitive, and highly reliable, making it a promising platform for efficient aptasensing.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32043411

RESUMO

The interaction between Tb(IV)-NR complex and herring sperm DNA in buffer solution of Tris-HCl was investigated with the use of acridine orange(AO) as a spectral probe. The binding modes and other information were provided by the UV-spectrophotometry and fluorescence spectroscopy. The thermodynamic functions expressed that the binding constants of Tb(IV)-NR complex with DNA was Kθ298.15K = 4.03 × 105 L·mol-1, Kθ310.15K =1.30 × 107 L·mol-1, and the ΔrGθ m 298.15 K=-3.20 × 104 J·mol-1. The scatchard equation suggested that the interaction mode between Tb(IV)-NR complex and herring sperm DNA is electrostatic and weak intercalation bindings. FTIR spectroscopy results also indicate that there is a specific interaction between the Tb(IV)-NR complex and the A and G bases of DNA.

3.
Technol Cancer Res Treat ; 19: 1533033819898728, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31960764

RESUMO

Recent studies have suggested that circular RNAs play an important role in the progression of various cancers. We aimed to investigate the possible role of cir-ITCH in osteosarcoma. In this study, we performed experiments with the human osteoblast cell line hFOB1.19 and several osteosarcoma cancer cell lines and the results showed that the expression of cir-ITCH in osteosarcoma cancer cell lines was significantly upregulated compared to that in the human osteoblast cell line. In addition, the results showed that cir-ITCH could promote the migration, invasion, and growth of osteosarcoma cells. Further mechanistic studies revealed that cir-ITCH could enhance epidermal growth factor receptor (EGFR) expression by reducing the level of miR-7. Increased EGFR phosphorylation was found to be concomitant with high expression of EGFR. We determined that cir-ITCH-mediated increase in the migration and invasion of osteosarcoma cells was dependent on EGFR phosphorylation. In conclusion, our research uncovered an important role of the cir-ITCH/miR-7/EGFR pathway in the migration and invasion of osteosarcoma cells and suggested that cir-ITCH may be a prognostic marker and a promising therapeutic target for osteosarcoma.

4.
Biomed Pharmacother ; 122: 109707, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31918278

RESUMO

Osteosarcoma (OS) is the most common type of bone cancer in children and adolescents. LncRNA differentiation antagonizing nonprotein coding RNA (DANCR) has been reported to be aberrant expression in osteosarcoma and contribute to proliferation, migration and invasion of cancer cells. Here, we further explore the exacted molecular mechanism of DANCR in OS. The expression of DANCR, microRNA-216a-5p (miR-216a-5p) and SOX5 was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Cells proliferation and apoptosis were analyzed by WST-1assay or flow cytometry, respectively. The migratory and invasion abilities were measured by transwell assay. Western blot was used to detect the level of SOX5 and autophagy-related protein of Beclin1, LC3-I and LC3-II. The interaction among DANCR, miR-216a-5p and SOX5 was explored by luciferase reporter assay, RIP assay or Pull-down assay. Murine xenograft model was established using 143B cells transfected with sh-DANCR. We found that a significantly elevated of DNACR was detected in osteosarcoma tissue and cell lines. Functional experiments suggested that down-regulation of DANCR inhibited cells proliferation, migration, invasion and autophagy but induced apoptosis in osteosarcoma in vitro. Additionally, we also determined knockdown of DANCR inhibited the growth and autophagy of osteosarcoma in vivo. DANCR was a sponge of miR-216a-5p activity. DANCR regulated survival of osteosarcoma through targeting miR-216a-5p. Additionally, SOX5 was a direct target of miR-216a-5p, overexpression miR-216a-5p exerted inhibition effects via down-regulating SOX5 expression. Furthermore, DANCR regulated SOX5 expression by sponging to miR-216a-5p. In conclusion, LncRNA DANCR silence inhibits SOX5-medicated progression and autophagy in osteosarcoma via regulating miR-216a-5p which indicating DANCR may act as a potential prognostic biomarker and therapeutic target for osteosarcoma.

5.
J Hazard Mater ; 383: 121178, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31525688

RESUMO

Organoarsenicals have been used in poultry production for years, however, studies focused on roxarsone (ROX), with little attention to p-arsanilic acid (ASA). We assessed arsenic (As) concentration and speciation in chicken meat collected from 10 cities in China. The geometric mean for total As in 249 paired raw and cooked samples was 4.85 and 7.27 µg kg-1 fw, respectively. Among 81 paired raw and cooked samples, ASA and ROX were detected in >90% samples, suggesting the prevalence of organoarsenical use in China. ASA contributed the most (45% on average) to total As in cooked samples, followed by As(V), DMA, As(III), and ROX (7.2-22%). ASA was found to contribute more to total As in chicken meat compared to ROX for the first time. Arsenic in chicken meat showed considerable geographic variation, with higher inorganic arsenic (iAs) being detected from cities with higher ROX and ASA, indicating that organoarsenical use increased iAs concentration in chicken meat. When health risk was estimated, dietary exposure to iAs would result in an increase of 3.2 bladder and lung cancer cases per 100,000 adults. The result supports the removal of organoarsenicals in poultry production from Chinese market and further supports its removal from the global markets.

6.
Plant Physiol ; 182(2): 908-918, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31843803

RESUMO

Flowering time plays a crucial role in the geographical adaptation of most crops during domestication. Cucumber (Cucumis sativus) is a major vegetable crop worldwide. From its tropical origin on the southern Asian continent, cucumber has spread over a wide latitudinal cline, but the molecular mechanisms underlying this latitudinal adaptation and the expansion of domesticated cucumber are largely unclear. Here, we report the cloning of two flowering time loci from two distinct cucumber populations and show that two large deletions upstream from FLOWERING LOCUS T (FT) are associated with higher expression of FT and earlier flowering. We determined that the two large deletions are pervasive and occurred independently in Eurasian and East-Asian populations. Nucleotide diversity analysis further revealed that the FT locus region of the cucumber genome contains a signature for a selective sweep during domestication. Our results suggest that large genetic structural variations upstream from FT were selected for and have been important in the geographic spread of cucumber from its tropical origin to higher latitudes.

7.
J Sci Food Agric ; 100(4): 1822-1826, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31858597

RESUMO

BACKGROUND: Koumiss is a traditional fermented beverage made from mare's milk. The traditional backslopping method for koumiss production has shortcomings in terms of microbiological diversity and nutritional characteristics. In this study, a two-stage fermentation method was established to scale up the production of koumiss powder. The chemical composition and nutritional properties of a novel two-stage fermentation koumiss powder (TKP) were compared with backslopping koumiss powder (BKP). RESULTS: The TKP exhibited important nutritional and functional properties, including a high percentage of essential amino acids, and high polyunsaturated fatty acid, vitamin, and mineral content. The essential amino acid content of TKP was not significantly different from that of BKP. The oleic acid, linoleic acid, linolenic acid, and water-soluble vitamin content of TKP was higher than that of BKP. The Ca:P ratio of TKP was also close to the optimal Ca:P ratio in humans. CONCLUSION: The novel method could be applied for the scaled-up production of koumiss powder with similar nutritional properties to traditional backslopping koumiss powder. The successful production of koumiss powder could also promote the development of the koumiss industry. © 2019 Society of Chemical Industry.


Assuntos
Microbiologia de Alimentos/métodos , Kumis/análise , Leite/microbiologia , Pós/química , Aminoácidos/análise , Animais , Fermentação , Cavalos , Kumis/microbiologia , Lactobacillaceae/metabolismo , Leite/química , Minerais/análise , Valor Nutritivo , Vitaminas/análise
8.
Transplant Proc ; 52(1): 392-397, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31862188

RESUMO

The protective effects of ischemic postconditioning on renal ischemia/reperfusion injury in mice and the role of miR-126 and Nrf2 signaling pathway in the process were the focus of this study. Mice were classified into 5 groups: sham-operation, I/R6h, I/R24h, postconditioning (POC), and miR-126 agomir. Serum creatinine, renal histopathology changes, and oxidative stress were examined. The expression of miR-126 and Nrf2 were detected. We also treated NRK52E cells with hypoxia reoxygenation. The I/R group showed significant renal injury and increased generation of oxidative stress. However, the severity of renal injury and oxidative stress were markedly attenuated in the POC group. MiR-126 was downregulated, and Nrf2 was upregulated in NRK52E cells subjected to hypoxia/reoxygenation (H/R) and in mouse kidneys subjected to POC. Oxidative stress was higher and Nrf2 was lower in the mouse miR-126 agomir group; apoptosis were higher in H/R-treated NRK52E cells transfected with Nrf2 siRNA and lower in anti-miR-126. These findings demonstrate that after renal ischemic postconditioning, miR-126 inhibits oxidative stress by inducing Nrf2 and suppresses injury.

9.
Br J Nutr ; : 1-12, 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31831094

RESUMO

We aimed to comprehensively examine the association of breast-feeding, types and initial timing of complementary foods with adolescent cognitive development in low- and middle-income countries. We conducted a prospective cohort study of 745 adolescents aged 10-12 years who were born to women who participated in a randomised trial of prenatal micronutrient supplementation in rural Western China. An infant feeding index was constructed based on the current WHO recommendations. Full-scale intelligence quotient (FSIQ) was assessed and derived by the fourth edition of the Wechsler Intelligence Scale for Children. The duration of exclusive or any breast-feeding was not significantly associated with adolescent cognitive development. Participants who regularly consumed Fe-rich or Fe-fortified foods during 6-23 months of age had higher FSIQ than those who did not (adjusted mean differences 4·25; 95 % CI 1·99, 6·51). For cows'/goats' milk and high protein-based food, the highest FSIQ was found in participants who initially consumed at 10-12 and 7-9 months, respectively. A strong dose-response relationship of the composite infant feeding index was also identified, with participants in the highest tertile of overall feeding quality having 3·03 (95 % CI 1·37, 4·70) points higher FSIQ than those in the lowest tertile. These findings suggest that appropriate infant feeding practices (breast-feeding plus timely introduction of appropriate complementary foods) were associated with significantly improved early adolescent cognitive development scores in rural China. In addition, improvement in Fe-rich or Fe-fortified foods complementary feeding may produce better adolescent cognitive development outcomes.

10.
J Hazard Mater ; : 121744, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31796360

RESUMO

In this study, the predominant Pb minerals prior to and after Pb relative bioavailability (Pb-RBA) and Pb bioaccessibility (Pb-BAc) tests were identified using SEM (scanning electron microscopy), XANES (X-ray absorption near edge structure) and XRD (X-ray diffraction). The correlations between in vitro Pb-BAc (using the UBM (Unified BARGE Method) and RBALP (Relative BioAccessibility Leaching Procedure) models) and in vivo Pb-RBA (using endpoints of kidney and liver in an mice model) were determined. The results demonstrated that both RBALP and UBM (gastric phase) reliably indicate Pb-RBA (Pb-RBA). However, raising the solid:liquid ratio of the gastric phase of UBM is necessary to determine Pb-BAc if the soils contain total Pb >10,000 mg/kg. The comparison of Pb minerals prior to and after in vitro extractions demonstrated that the relatively soluble forms of Pb (PbSO4, PbO2 and MgO Pb) start to dissolve than other forms of Pb minerals, suggesting there was no difference in Pb2+ release between chemical-based (RBALP) and physiologically-based (UBM) models. The identification of the Pb minerals of Pb5(PO4)3Cl and organically-complexed Pb in mice excreta demonstrated that a portion of Pb2+ combined with food and humic acid to generate organically-complexed Pb in mice excreta, and that Pb5(PO4)3Cl is not bioavailable.

11.
Biomed Res Int ; 2019: 5049248, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31886221

RESUMO

Background: Rapid on-site evaluation (ROSE) is commonly used to evaluate the adequacy of biopsy materials in fine-needle aspiration; however, the diagnostic performance of ROSE during fiber optic bronchoscopy (FOB) biopsy under direct vision is rarely reported. Here, we evaluated the role of ROSE during FOB biopsy of visible lesion in trachea or bronchi. Methods: The role of ROSE was prospectively evaluated in consecutive bronchoscopy specimens obtained between January 2016 and January 2018. The agreement and accuracy between ROSE and final histopathological interpretation were assessed. The frequency and possible reasons for discrepancy between ROSE and definitive histopathology results were identified. Histological and cytological classification was performed according to the International Association for the Study of Lung Cancer, the American Thoracic Society, and the European Respiratory Society (IASLC/ATS/ERS) criteria of lung ADCs classification. Results: The study enrolled 651 patients, of which 33 were excluded because of insufficient cells. Final diagnosis of malignancy was achieved in 462 cases (74.8%), whereas 156 cases (25.2%) were nonmalignant. ROSE and pathology were well correlated for the diagnosis of squamous cell carcinoma (SCC) (Kappa = 0.718, p < 0.05), adenocarcinoma (AdC) (Kappa = 0.662; p < 0.05) and small cell lung cancer (SCLC) (Kappa = 0.955; p < 0.05). In 24 cases diagnosed as malignant by ROSE and nonmalignant by pathology, the lesion tissues were surgically excised and re-analyzed, and the 24 cases were finally confirmed as malignant by pathology. Conclusions: ROSE technique allows bronchoscopists to obtain viable and adequate material for the diagnosis of histopathology, and provides them with an onsite preliminary diagnosis especially in cases with inconclusive macroscopic appearance. ROSE and pathology should be used in combination to increase the accuracy of diagnosis.

12.
Chem Biol Drug Des ; 2019 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-31883507

RESUMO

This study investigated the mechanisms through which arctigenin promotes osteogenesis. Bone marrow mesenchymal stem cells (BMSCs) from ovariectomized (OVX) rats were differentiated into osteoblasts, and osteogenesis was evaluated via Alizarin Red S (ARS) staining and alkaline phosphatase (ALP) measurements in cultured BMSCs. The levels of phosphorylated AKT serine/threonine kinase 1 (p-Akt), and peroxisome proliferator-activated receptor gamma (PPARγ) expression were quantified by Western blot analysis. The levels of urine calcium (U-Ca), urine phosphorus (U-P), serum ALP, and bone mineral density (BMD) of OVX rats were assessed in vivo. The results showed that treatment with arctigenin in rat BMSCs enhanced mineralization, increased ALP activity, increased the expression of Akt and p-Akt, and decreased PPARγ expression, consistent with its ability to promote osteoblast differentiation. Furthermore, arctigenin prevented OVX-induced osteoporosis in rats by increasing BMD and ALP activity and inhibiting the loss of Ca and P. In contrast, treatment with LY294002, a selective inhibitor of the phosphatidylinositol 3-kinase (PI3K), produced the opposite phenotype. These data suggest that the protective effects of arctigenin on BMSCs and OVX rat models result from the induction of osteogenesis involving the PI3K/Akt/PPARγ axis.

13.
ACS Appl Mater Interfaces ; 11(51): 48551-48555, 2019 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-31782302

RESUMO

The photophysics of charge-transfer and recombination mechanisms in a heterojunction structure of CdSe/CdS/Au quantum dots (QDs) are studied by temperature-dependent steady-state photoluminescence (PL) and time-resolved PL (TRPL). We manipulate the charge transfer from core to shell surface by varying the tunneling barrier height resulting from temperature variation and the barrier width resulting from shell thickness variation. The charge-transfer process, which can be described by a tunneling transmission model, is manifested by two competitive recombination processes, an intrinsic exciton emission and a trap emission in the near-infrared (NIR) range. Our study establishes the photophysics foundation for the core/shell/metal application in photocatalyst and optoelectronics.

14.
J Pediatr Surg ; 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31759656

RESUMO

BACKGROUND: Delayed diagnosis of children with Bronchial Foreign Body (BFB) leads to significant pulmonary complications and open surgery may not be avoided. However, surgical management for children with BFB is rarely reported. This study aims to describe our experience in the diagnosis and surgical treatment of late-diagnosed BFB during childhood. METHODS: Medical records of 8 children who were diagnosed with BFB and underwent open surgery at Children's Hospital of Chongqing Medical University between January 2004 and June 2019 were retrospectively reviewed. RESULTS: This group consisted of 8 children, with an average age of 8.1 years. In this group, the typical aspiration history was absent and the diagnosis of BFB was established in delay. The onset of diseases varied from 2 months to over 4 years. Lobectomy was performed in 7 patients and pneumonotomy was performed in 1 patient. No postoperative death was found. The clinical outcomes were satisfactory with an average 33 months follow-up. CONCLUSIONS: The diagnosis of BFB should be considered in children who present with repeated pneumonia and agnogenic bronchiectasis and actelectasis despite repeated medical treatment. Surgical treatment is necessary and effective in patients with either unextractable BFB or irreversible damage of lung tissue. LEVELS OF EVIDENCE: Level IV. TYPE OF STUDY: Retrospective study.

15.
J Cell Physiol ; 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31667864

RESUMO

Lupus nephritis (LN) is the most common complication of systemic lupus erythematosus. Patients with LN mostly die of sclerosing glomerulonephritis and renal failure. The inhibition of glomerular mesangial matrix deposition is an efficient method to restrict the progress of renal injury. By recognizing and binding extracellular and intracellular ligands, Toll-like receptor 2 (TLR2) contributes to the pathogenesis of most immune diseases. However, the relationship between TLR2 and LN is still unknown. Our previous studies confirmed that high-mobility group box 1 (HMGB1), an important ligand of TLR2, promotes the progression of LN by inducing the proliferation of glomerular mesangial cells. However, whether or not HMGB1 participates in the pathogenesis of glomerular mesangial matrix deposition in LN remains unknown. In this study, we observed the upregulated expression of TLR2 in the glomeruli of LN patients and MRL/lpr mice. The inhibition of either TLR2 or HMGB1 inhibited the release of fibronectin and the activation of the MyD88/NF-κB pathway in mesangial cells cultured with LN plasma. In addition, both TLR2- and HMGB1-deficient mice showed reduced 24 hr urine protein levels and improved glomerular histological changes and sclerosis levels. These results indicate that TLR2 regulates glomerular mesangial matrix deposition in LN through the activation of the MyD88/NF-κB pathway by binding to HMGB1.

16.
Mol Med Rep ; 20(5): 4695-4705, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702022

RESUMO

Treatment of cancer­induced bone pain (CIBP) is challenging in clinical settings. Oxycodone (OXY) is used to treat CIBP; however, a lack of understanding of the mechanisms underlying CIBP limits the application of OXY. In the present study, all rats were randomly divided into three groups: The sham group, the CIBP group, and the OXY group. Then, a rat model of CIBP was established by inoculation of Walker 256 tumor cells from rat tibia. Phosphoproteomic profiling of the OXY­treated spinal dorsal cords of rats with CIBP was performed, and 1,679 phosphorylated proteins were identified, of which 160 proteins were significantly different between the CIBP and sham groups, and 113 proteins were significantly different between the CIBP and OXY groups. Gene Ontology analysis revealed that these proteins mainly clustered as synaptic­associated cellular components; among these, disks large homolog 3 expression was markedly increased in rats with CIBP and was reversed by OXY treatment. Subsequent domain analysis of the differential proteins revealed several significant synaptic­associated domains. In conclusion, synaptic­associated cellular components may be critical in OXY­induced analgesia in rats with CIBP.

17.
Environ Sci Technol ; 53(24): 14264-14272, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31731833

RESUMO

Soils are often co-contaminated with As, Pb, and Cd. To what extent ingested metal(loid)s interact with each other in the gastrointestinal tract and influence their RBA (relative bioavailability) is largely unknown. Three soils predominantly contaminated with As (MS, mining/smelting impacted), Pb (WR, wire rope production impacted), and Cd (EP, enamel pottery production impacted) were administered to mice individually or in binary and tertiary combinations with sodium arsenate, Cd chloride, and/or Pb acetate. In binary combinations, ∼10-fold higher Pb addition decreased As-RBA in MS (26.0 ± 6.28% to 17.1 ± 1.08%), while ∼10-fold higher As addition decreased Pb-RBA in WR (61.3 ± 2.41% to 28.8 ± 5.45%). This was possibly due to the formation of insoluble Pb arsenate in mouse intestinal tract, as indicated by the formation of precipitates when As and Pb co-occurred in water or simulated human gastrointestinal fluids. Due to competition for shared absorption transporters, ∼10- and 100-fold higher Pb addition decreased Cd-RBA in EP (95.8 ± 12.9% to 67.8 ± 12.8% and 62.8 ± 8.24%). Tertiary combinations showed that interactions between two metal(loid)s were affected by the presence of the third metal(loid). Our study suggests that As oxyanion could interact with Pb or Cd ions in the mouse gastrointestinal tract, and the interactions vary depending on concentration and solution characteristics.

18.
PeerJ ; 7: e7816, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31608178

RESUMO

Objective: Hepatocellular carcinoma (HCC) is a disease that is associated with high mortality; currently, there is no curative and reliable treatment. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) is the key signaling molecule in the wingless and INT-1/planar cell polarity (WNT/PCP) pathway. This study aimed to elucidate the prognostic significance of CELSR3 in HCC patients. Methods: The Cancer Genome Atlas (TCGA) database, the Cancer Cell Line Encyclopedia (CCLE) database and the Gene Expression Omnibus (GEO) database were used to analyze the expression of CELSR3 mRNA in HCC samples and cells. The relationship between CELSR3 mRNA and clinical features was assessed by the chi-square test. the diagnostic and predictive value of CELSR3 mRNA expression were analyzed using the receiver operating characteristic (ROC) curve. Kaplan-Meier curve and Cox regression analyses were performed to assess the prognostic value of CELSR3 mRNA in HCC patients. Finally, all three cohorts database was used for gene set enrichment analysis(GSEA) and the identification of CELSR3-related signal transduction pathways. Results: The expression of CELSR3 mRNA was upregulated in HCC, and its expression was correlated with age (P = 0.025), tumor status (P = 0.022), clinical stage (P = 0.003), T classification (P = 0.010), vital status (P = 0.001), and relapse (P = 0.005). The ROC curve assessment indicated that CELSR3 mRNA expression has high diagnostic value in HCC and in the subgroup analysis of stage. In addition, the Kaplan-Meier curve and Cox analyses suggested that patients with high CELSR3 mRNA expression have a poor prognosis, indicating that CELSR3 mRNA is an independent prognostic factor for the overall survival of HCC patients. GSEA showed that GO somatic diversification of immune receptors, GO endonuclease activity, GO DNA repair complex and GO somatic cell DNA recombination, were differentially enriched in the meta-GEO cohort, the HCC cell line cohort and the TCGA cohort of the high CELSR3 mRNA expression phenotype. Conclusion: Our results indicate that CELSR3 mRNA is involved in the progression of cancer and can be used as a biomarker for the prognosis of HCC patients.

20.
ACS Sens ; 4(10): 2724-2729, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31564103

RESUMO

Trace concentration of formaldehyde can damage human health and environment. Consequently, it is of great significance to develop an ultrasensitive sensor for its determination. Herein, an ingenious and efficient photoelectrochemical sensor for formaldehyde was constructed by amorphous TiO2 hollow spheres incorporated with Ag+ ions, which were brought about by silica template etching and then the exchange of Ag+/Na+ ions. The amorphous TiO2 acted the dual role of Ag+ ion probe carriers and photoelectric materials. Upon exposure to the increased concentration of formaldehyde, the Ag nanoparticles were produced in situ, and photocurrent amplification was then achieved in a proportional manner. It is attributed to the injection of hot electrons from plasmonic Ag nanoparticles into the conduction band of amorphous titanium dioxide and therefore enhanced the photocurrent. The linear relationship between 1 and 400 pmol L-1 resulted from the enhanced photocurrent and increased concentration of formaldehyde, and the detection limit was 0.4 pmol L-1. Benefiting from an in situ and unique sensitization strategy, this photoelectrochemical sensor exhibited many advantages such as sensitivity, selectivity, cost-effectiveness, convenience of fabrication, low power consumption, and stability.

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