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1.
J Environ Manage ; 255: 109881, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31778870

RESUMO

Pollutant concentrations in influents into constructed wetlands (CWs) are highly fluctuating and may vary over several orders of magnitude, leading to large uncertainties in removal performance assessment when using pollutant concentrations in the influent and effluent directly. Incorporating a probabilistic approach into removal performance assessment and needed area estimation of CWs could advantage decision making regarding wastewater treatment and engineering applications. A series of three-stage surface-flow CWs (SFCWs) were constructed for treating ammonium-rich swine wastewater. The surface removal rate and removal efficiency of ammonium nitrogen in the SFCWs using the probabilistic approach were 0.27-3.23 g m-2 d-1 and 43.0-99.9% (95% confidence interval (CI)), which were consistent with the deterministic approach (95% CI: 0.24-3.18 g m-2 d-1 and 70.4-99.9%). The needed SFCW area was estimated as 6.6 (95% CI: 1.4-17.8) to 29.7 (95% CI: 6.4-80.1) m2 for required removal efficiency from 40% to 90% for 0.18 m3 d-1 swine wastewater with different strengthens. For specific removal efficiency of 90%, the needed CW areas was 13.9 (95%CI: 4.9-42.7), 25.1 (95%CI: 5.9-66.0), 33.5 (95%CI: 13.5-87.1), and 40.8 (95%CI: 16.2-89.4) m2 for influent ammonium loading rate of 0.18-2.7, 2.7-14.4, 14.4-36, and 36-60 g d-1, respectively. The first-order removal constant of ammonium nitrogen decreased logarithmically with increasing influent and effluent concentration/loading rate in the SFCW units (p < 0.001), which was responsible for the needed SFCW areas covering a wide range. The reliability analysis confirmed the results from the probabilistic approach were appropriate. The present study shed new lights on the performance evaluation and design of CWs for treating wastewater with highly-fluctuating concentrations using a probabilistic approach.

2.
Org Lett ; 21(23): 9457-9462, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31755725

RESUMO

Pd-catalyzed regio- and enantioselective allylic etherification of vinylethylene carbonates (VECs) with diols has been developed. By using cooperative catalysts of the chiral palladium complex and triethylborane in mild conditions, the process gave monoetherified and bisetherified polyglycol derivatives with tetrasubstituted stereocenters in high yields with complete regioselectivities and high levels of enantio- and diastereoselectivities.

3.
Sci Total Environ ; 694: 133575, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31756813

RESUMO

Exploring optimal C:N ratio is necessary to ensure balanced microbial nitrification and denitrification in constructed wetlands (CWs), which has become an important management practice for more efficient nitrogen removal and sustainability of CWs. Surface flow constructed wetlands (SFCWs) vegetated with Myriophyllum aquaticum were designed to investigate the effects of five different influent C:N ratios (0:1, 2.5:1, 5:1, 10:1, and 15:1) on nitrogen removal performance and microbial communities over a 175-day experimental period. Compared to the influent C:N ratios of 0:1, higher NH4+-N, NO3--N, and total nitrogen (TN) removal efficiencies and lower NO3--N accumulation were observed at influent C:N ratios higher than 5:1. In addition, the highest TN removal efficiency (70.4%) and the lowest nitrous oxide emission flux (4.12 mg m-2 d-1) were obtained at the influent C:N ratio of 5:1. High-throughput sequencing revealed that influent C:N ratios altered the distribution and composition of microbial communities in the sediment, which resulted in a dynamic interplay between N-transforming functional microbes and NH4+-N and NO3--N removal. In particular, the dominant denitrifiers, including Desulfovibrio, Zoogloea, and Dechloromonas, were more abundant in the sediment with an influent C:N ratio of 5:1, which contributed to the high N removal rate. These findings may be used to screen for an optimum influent C:N ratio to maintain the sustainability of SFCWs with higher N removal efficiency.


Assuntos
Eliminação de Resíduos Líquidos/métodos , Áreas Alagadas , Carbono , Nitrogênio , Microbiologia da Água
4.
Artif Cells Nanomed Biotechnol ; 47(1): 3961-3975, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31588802

RESUMO

Ion-complementary self-assembling peptides have potential in delivering hydrophobic drugs. This study involved two self-assembling peptides, RADA16-I and RVDV16-I, of which RVDV16-I was a novel self-assembling peptide with different hydrophobic side chains designed from RADA16-I. The purpose of this study was to observe the interaction between different self-assembling peptides and emodin through fluorescence spectrophotometry, CD, SEM and AFM; to construct a preliminary suspension in-situ hydrogel delivery system for emodin with the self-assembling peptides; and to investigate the drug-loading and drug-releasing properties of the self-assembling peptides on emodin. The results showed that both peptides can interact with emodin and the interaction was dominated by hydrophobic interaction. The aqueous solutions of both self-assembling peptides can form relatively stable suspensions with emodin under mechanical stirring, and the suspension can form in-situ hydrogel under physiological condition. In vitro release of emodin from the hydrogels showed a manner of sustained release to some extent. Cell viability studies showed inherent proliferation inhibiting effects of emodin on tumor cells was maintained or enhanced through the in-situ hydrogels. The self-assembling peptides RADA16-I and RVDV16-I had showed promising drug-loading and drug-releasing performance for hydrophobic drugs. It is reasonable to exploit self-assembling peptides as drug carriers for their great potential to improve delivery of hydrophobic drugs.

5.
Chem Commun (Camb) ; 55(78): 11687-11690, 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31508614

RESUMO

A series of supramolecular assemblies were constructed using decamethylcucurbit[5]uril. Coordination of an alkali metal and further linkage by [PtCl6]2- created a 1D building block, which formed a 3D structure through abundant hydrogen bonds. The obtained supramolecular assemblies exhibited excellent electrocatalytic performance towards the oxygen reduction reaction, comparable to the commercial Pt/C catalyst. Full characterizations, as well as density functional theory calculation results, demonstrated the structure-performance relationship.

6.
Org Biomol Chem ; 17(35): 8075-8078, 2019 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-31460559

RESUMO

An efficient method for the construction of arylated allylic ethers was developed via three-component tandem arylation and allylic etherification of 2,3-allenol with aryl iodides and alcohols. In the cooperative catalytic system of a palladium complex and triethylborane, the process allows rapid access to functionalized 1-arylvinylated 1,2-diol derivatives in good to high yields with complete branch-selectivities. The synthetic utility of the present process was demonstrated by the late-stage functionalization of a drug molecule, the gram-scale synthesis and the elaboration of the products.

7.
Anal Bioanal Chem ; 411(25): 6755-6765, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31388716

RESUMO

Diphacinone (DPN) is an extensively used anticoagulant rodenticide that is also considered a hazardous chemical, which poses a threat to nontarget species. DPN poisoning cases in humans or other species frequently occur, while rapid and sensitive detection methods are rarely reported. Thus, it is meaningful to develop an immunoassay for DPN detection with high sensitivity and specificity. In this study, a hapten was synthesized and then conjugated with carrier proteins to prepare the immunogens with different conjugation ratios for the preparation of antibody. After evaluation of the antisera using an indirect competitive enzyme-linked immunosorbent assay (icELISA) and statistical analysis, we found that the immunogen prepared using the N,N-dicyclohexylcarbodiimide (DCC) method with a conjugation ratio of 28.5 could elicit mice to generate antibodies with high performance. Using hybridoma technology, we obtained the specific monoclonal antibody (mAb) 4G5 with a half maximal inhibitory concentration (IC50) of 0.82 ng/mL in buffer solution. We initially explored the recognition mechanism of DPN/CLDPN and mAb from both conformational and electronic aspects. Then, mAb 4G5 was applied to develop icELISA for biological samples. The limits of detection (LODs) of icELISA were 0.28 µg/L, 0.32 µg/L, and 0.55 µg/kg for swine plasma, urine, and liver samples, respectively, and the recoveries ranged from 72.3 to 103.3% with a coefficient of variation (CV) of less than 12.3% in spiked samples. In summary, we developed a sensitive, specific, and accurate icELISA for the detection of DPN in biological samples, which showed potential in food safety analysis and clinical diagnosis. Graphical abstract.


Assuntos
Anticoagulantes/análise , Ensaio de Imunoadsorção Enzimática/métodos , Fenindiona/análogos & derivados , Rodenticidas/análise , Animais , Anticorpos Monoclonais/imunologia , Formação de Anticorpos , Anticoagulantes/sangue , Anticoagulantes/imunologia , Anticoagulantes/urina , Feminino , Limite de Detecção , Fígado/química , Camundongos Endogâmicos BALB C , Modelos Moleculares , Fenindiona/análise , Fenindiona/sangue , Fenindiona/imunologia , Fenindiona/urina , Rodenticidas/sangue , Rodenticidas/imunologia , Rodenticidas/urina , Suínos
8.
Chem Commun (Camb) ; 55(66): 9805-9808, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31360985

RESUMO

Ultra-small Pd nanoparticles were successfully prepared through reduction of a supramolecular assembly formed between the macrocyclic decamethylcucurbit[5]uril (Me10CB[5]) and [PdCl4]2- anions via H-bonds. The final Pd NPs exhibit excellent activity and stability toward the electrochemical reduction of CO2 to CO. This work provides a new strategy for the preparation of nanocatalysts.

9.
J Neuroimmunol ; 334: 576978, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31177033

RESUMO

Alzheimer's disease (AD) is a common neurodegenerative disorder characterized by progressive learning and cognitive damage. Several hypotheses such as amyloid cascade hypothesis, hyper-phosphorylated τ hypothesis, and energy metabolism hypothesis have been proposed to elucidate the disease. However, the exact mechanism of AD remains unclear and current therapeutic strategies are miserable. Cumulative evidence showed that neuroinflammation plays a significant role in the pathogenesis of the AD. Oxymatrine (OMT), a plant-derived bioactive compound, has anti-viral, anti-fibrosis, and anti-tumor effects through the involvement of several immune-related signaling pathways. Whether OMT can attenuate the pathology of AD is largely unknown. In this manuscript, we found that treatment of OMT can significantly improve cognitive and learning abilities of AD mice during various behavioral test. Treatment of OMT can significantly reduce the densities of Aß plaques and astrocyte clusters in the neocortex and hippocampus of AD mice. Furthermore, treatment of OMT significantly reduced the concentration of pro-inflammatory cytokines including IL-6, IL-1ß, TNF-α and IL-17A in AD mice. Taken together, our data indicate that OMT may serve as a potential drug for AD.

10.
Anal Biochem ; 581: 113336, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31201790

RESUMO

Enzyme labeling of an antigen or an antibody helps to visualize and amplify the signal and is an important reagent used in immunoassays for the detection of a target of interest. In this research, soybean peroxidase (SBP), a less commonly used enzyme reporter, was compared in immunoassays with the two most commonly used reagents, horseradish peroxidase (HRP) and alkaline phosphatase (ALP). The enzyme-antibody conjugates were evaluated by their performance in an indirect competitive enzyme-linked immunosorbent assay (icELISA) and in an indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) for ractopamine (RAC). The results revealed that the more affordable SBP offers a long-lasting chemiluminescent signal, which outperformed ALP and HRP. SBP-antibody conjugate (SBP-Ab) based immunoassays produced lower limits of detection (LODs) and better accuracy in the detection of RAC in animal urine samples. Additionally, SBP-Ab has advantages in being more resistant to heat, acid and organic solvents. These results suggest that SBP could be a potentially excellent alternative to HRP and ALP for the development of immunoassay in food safety field.

11.
Huan Jing Ke Xue ; 40(5): 2194-2201, 2019 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-31087856

RESUMO

Based on the discharge characteristics of agricultural non-point source pollution in the headstream region of the Kaihui River-a typical small agricultural watershed, an integrated ecological engineering treatment system (IEETS) was constructed with ecological wetland as the core unit to control the pollution. The nitrogen (N) and phosphorus (P) removal performances of the IEETS were discussed in this study. The results showed that the dominant source of N and P in the study area was livestock and poultry breeding, which was urgent to control. The monitoring results indicated that the ecological wetland treatment project resulted in average total nitrogen (TN) and total phosphorus (TP) removal rates of 87.1% and 90.9%, respectively, when treating mixed decentralized domestic and swine wastewater treatment. The multi-stage constructed wetlands had an average of 85.7% of TN and 84.9% of TP removal for mixed farmland drainage and decentralized swine wastewater. The removal rates for landscape-based ecological wetland were within the range of 27.1%-67.4% for TN and 13.3%-81.5% for TP in the catchment terminal water. The total interception amount of TN and TP by the IEETS was 5292 kg·a-1 and 1054 kg·a-1, accounting for 35.3% and 43.6% of total pollution loads in the headstream region, respectively. These findings illustrated that the IEETS presents promising treatment results on non-point source pollution, and is suitable for widespread applications to wastewater treatment in small watersheds of southern China.

12.
Bosn J Basic Med Sci ; 19(3): 274-281, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31136293

RESUMO

Long non-coding RNAs (lncRNAs) are emerging as important modulators of cancer progression, among which prostate cancer-associated transcript 1 (PCAT1) has been shown to be an oncogene in several tumors. However, the clinical significance and biological function of PCAT1 in endometrial carcinoma (EC) remain unclear. In this study, we used 89 EC tissues and HEC-1B, Ishikawa, RL95-2 and AN3CA EC cell lines. We found elevated expression levels of PCAT1 in EC tissues and cell lines using reverse transcription qPCR (RT-qPCR). The prognostic value of PCAT1 was determined using Kaplan-Meier survival and Cox regression analysis. The results showed that higher PCAT1 expression was positively correlated with FIGO stage, myometrial invasion, lymph node metastasis, and a shorter overall survival. A series of functional assays showed that the knockdown of PCAT1 by small interfering RNA (siRNA) targeting PCAT1 (siPCAT1) suppressed cell proliferation, migration and invasion, but promoted apoptosis. Western blot analysis further showed that B-cell lymphoma 2 (Bcl-2), vimentin and N-cadherin were downregulated, but E-cadherin and Bcl-2-associated death promoter (Bad) were upregulated in PCAT1-silenced EC cells. Taken together, our results underscore the oncogenic role of PCAT1 in EC and show that PCAT1 may be a potential therapeutic target in EC treatment.

13.
Food Chem ; 294: 347-354, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31126473

RESUMO

Immunoassays with ultra-high sensitivity for the rapid detection of chemical contaminants in food are urgently required. However, conventional enzyme-linked immunosorbent assay (ELISA) usually suffer from the moderate sensitivity. Herein, we aim to improve the sensitivity of conventional ELISA by employing the fluorescent carbon dots (CDs) as the signal probes based on the principle of inner filter effect (IFE). In this strategy, the enzymatically formed products of horseradish peroxidase/alkaline phosphatase efficiently quenched the CDs via the IFE. The absorption signal of the conventional ELISA was converted into the fluorescence signal. The fluorescent immunoassay was successfully developed and used to detect amantadine residues in chicken, achieving a limit of detection of 0.02 ng mL-1. The fluorescent immunoassay is a straightforward, extendable and general strategy and exhibits potential in detecting trace amounts of chemical contaminants in foodstuff.


Assuntos
Amantadina/análise , Imunoensaio/métodos , Pontos Quânticos/química , Fosfatase Alcalina/metabolismo , Amantadina/imunologia , Animais , Carbono/química , Galinhas/metabolismo , Análise de Alimentos , Peroxidase do Rábano Silvestre/metabolismo , Limite de Detecção , Espectrometria de Fluorescência
14.
Micromachines (Basel) ; 10(3)2019 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-30909638

RESUMO

Silver nanowires (AgNW) have excellent electrical conductivity, transparency, and flexing endurance, and are broadly used in flexible electrodes and flexible sensors. This study mixed the silver nanowires and polyimide (PI) polymer using an in situ synthesis method, effectively reducing the problem of silver nanowires falling off the substrate. The selective wet etching method was firstly used to process the surface of AgNW-PI films, greatly enhancing the surface conductivity of AgNW-PI films. A flexible pressure sensor with high sensitivity was designed with two face-to-face AgNW-PI ultrathin layers. The experimental results show that our sensor presented a high sensitivity of about 1.3294 kPa-1 under a pressure of about 600 Pa, and when pressure continued to increase, the sensitivity decreased rapidly and reached saturation. Our flexible pressure sensor has the properties of low cost, high sensitivity, excellent repeatability, durability, and can detect various types of mechanical forces which could be utilized for flexible electronics.

15.
Public Health Nurs ; 36(4): 575-586, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30883888

RESUMO

BACKGROUND: Diabetes is a common chronic disease that requires a long-term regimen. However, the management of diabetes by telenursing is limited and inconclusive. OBJECTIVES: To determine the effectiveness of telenursing on control in diabetes. We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs). METHODS: We searched electronic databases, including PubMed, Embase, Web of Science and Cochrane Library. Studies comparing telenursing with usual care in diabetes patients were included. RESULTS: A total of 17 randomized controlled trials were identified. Glycated hemoglobin (HbA1c) dates were pooled using a random effects meta-analysis method, followed by subgroup analyses to examine heterogeneity. The meta-analysis showed that the use of telenursing (vs. usual care) was associated with a significant reduction in HbA1c levels compared to usual care, with a pooled 0.68% (95% CI: 0.33-1.03, p = 0.0001; I2  = 95%). For the secondary outcome, the SMD of body mass index (BMI) was -0.25% (95% CI: -0.81 to 0.32%, p = 0.39), with no statistically significant change; the fasting blood sugar (FBS) SMD was -0.19% (95% CI: 0.20 to 1.01, p = 0.003), with a statistically significant change; the total cholesterol (TC) SMD was -0.09% (95% CI: -0.03 to 0.21, p = 0.12), with no statistically significant change. CONCLUSIONS: Telenursing, as a useful tool for patient education and behavioral interventions, can help diabetes patients to improve their glycemic control. However, more studies on up-to-date and cost-effective technologies are needed.

16.
Future Oncol ; 15(14): 1577-1591, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30859853

RESUMO

Aim: Research on novel mutant genes may develop the treatment of cervical cancer (CC). The role of miRNA-526b in epithelial-mesenchymal transition (EMT) of CC was investigated. Methods: The role and the molecular mechanism of miRNA-526b in CC and its effect on EMT were analyzed in clinical specimens and oncology experiments. Results: miRNA-526b was proved to be decreased in CC and associated with malignant clinicopathological characters. The character of miRNA-526b in EMT was also inspected in CC cells and tumor models. miRNA-526b was found to be able to inhibit the EMT property of CC cells by directly targeting PBX3. Conclusion: miRNA-526b restoration may be deliberated as a new treatment strategy of CC.


Assuntos
Transição Epitelial-Mesenquimal/genética , Regulação Neoplásica da Expressão Gênica , Proteínas de Homeodomínio/genética , MicroRNAs/genética , Proteínas Proto-Oncogênicas/genética , Interferência de RNA , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/patologia , Regiões 3' não Traduzidas , Adulto , Idoso , Animais , Movimento Celular/genética , Proliferação de Células/genética , Modelos Animais de Doenças , Feminino , Humanos , Camundongos , Pessoa de Meia-Idade , Metástase Neoplásica , Estadiamento de Neoplasias
17.
Org Lett ; 21(1): 214-217, 2019 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-30556697

RESUMO

An efficient method for the enantioselective construction of tertiary 1,3-diols via Pd-catalyzed asymmetric allylic cycloaddition of vinyloxetanes with an abundant feedstock, formaldehyde, is developed. Using the palladium complex generated in situ from Pd2(dba)3·CHCl3 and phosphoramidite L3 as a catalyst under mild conditions, the process allows one to convert racemic 2-substituted 2-vinyloxetanes (1) to the corresponding 1,3-dioxanes (2) as methylene acetal protected tertiary 1,3-diols in high yields with good to excellent enantioselectivities.

18.
Food Chem ; 271: 9-17, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30236746

RESUMO

In this study, 10 fluorescein-labeled ractopamine (RAC) derivatives (tracers) were synthesized and characterized to develop a rapid fluorescence polarization immunoassay (FPIA) for the detection of RAC in pork, using previously produced RAC polyclonal antibodies. The effect of the tracer structure on the sensitivity of the FPIA was investigated. The specificity of the FPIA was evaluated with 70 ß-agonists and ß-blockers. The FPIA showed a limit of detection of 0.56 µg kg-1 for RAC in pork, with recoveries ranging from 74.8% to 86.6% in spiked samples. The total analysis time, including sample pretreatment, was less than 1 h. The FPIA was used to screen 150 commercial pork samples for RAC residues and the results were consistent with those of an enzyme-linked immunosorbent assay (ELISA) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Our results demonstrate that the FPIA developed here is a rapid, accurate, and sensitive screening method for RAC residues in pork.


Assuntos
Imunoensaio de Fluorescência por Polarização/métodos , Contaminação de Alimentos/análise , Fenetilaminas/isolamento & purificação , Carne Vermelha/análise , Animais , Suínos , Espectrometria de Massas em Tandem
19.
Oxid Med Cell Longev ; 2018: 6069150, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30581534

RESUMO

The aim of this study was to investigate whether uric acid (UA) might exert neuroprotection via activating the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway and regulating neurotrophic factors in the cerebral cortices after transient focal cerebral ischemia/reperfusion (FCI/R) in rats. UA was intravenously injected through the tail vein (16 mg/kg) 30 min after the onset of reperfusion in rats subjected to middle cerebral artery occlusion for 2 h. Neurological deficit score was performed to analyze neurological function at 24 h after reperfusion. Terminal deoxynucleotidyl transferase-mediated dNTP nick end labeling (TUNEL) staining and hematoxylin and eosin (HE) staining were used to detect histological injury of the cerebral cortex. Malondialdehyde (MDA), the carbonyl groups, and 8-hydroxyl-2'-deoxyguanosine (8-OHdG) levels were employed to evaluate oxidative stress. Nrf2 and its downstream antioxidant protein, heme oxygenase- (HO-) 1,were detected by western blot. Nrf2 DNA-binding activity was observed using an ELISA-based measurement. Expressions of BDNF and NGF were analyzed by immunohistochemistry. Our results showed that UA treatment significantly suppressed FCI/R-induced oxidative stress, accompanied by attenuating neuronal damage, which subsequently decreased the infarct volume and neurological deficit. Further, the treatment of UA activated Nrf2 signaling pathway and upregulated BDNF and NGF expression levels. Interestingly, the aforementioned effects of UA were markedly inhibited by administration of brusatol, an inhibitor of Nrf2. Taken together, the antioxidant and neuroprotective effects afforded by UA treatment involved the modulation of Nrf2-mediated oxidative stress and regulation of BDNF and NGF expression levels. Thus, UA treatment could be of interest to prevent FCI/R injury.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/metabolismo , Infarto Cerebral/tratamento farmacológico , Infarto Cerebral/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Ácido Úrico/uso terapêutico , Animais , Western Blotting , Desoxiguanosina/análogos & derivados , Desoxiguanosina/metabolismo , Ensaio de Imunoadsorção Enzimática , Heme Oxigenase-1/metabolismo , Imunoquímica , Marcação In Situ das Extremidades Cortadas , Masculino , Malondialdeído/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley
20.
PLoS One ; 13(10): e0204499, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30273360

RESUMO

Resveratrol, a non-flavonoid polyphenolic compound, is structurally and functionally similar to estrogen and has drawn great attention for its potentially beneficial effects on diabetes. However, it is not known whether it shares the same protective effect against diabetes as estrogen and the underlying mechanisms. The aim of the present study was to investigate the protective effects of phytoestrogen resveratrol and exogenous 17ß-estradiol against streptozotocin (STZ)-induced type 1 diabetes. Female mice were ovariectomized (OVX) and chronically injected with different concentrations of resveratrol (0.1, 1 or 10 mg/kg) and 17ß-estradiol (0.01, 0.1 or 1 mg/kg) subcutaneously for 4 weeks, and the levels of blood glucose, plasma insulin, plasma antioxidant capacity, the changes of pancreatic islet cells and the expressions of glucose transporter 4 (GLUT4), insulin receptor substrate 1 (IRS-1) and phosphorylation of extracellular signal-regulated kinase (p-ERK) were detected. Resveratrol and 17ß-estradiol significantly inhibited the increase of the blood glucose level and the rise of plasma malondialdehyde in STZ-induced diabetic mice, improved the levels of plasma antioxidant capacity and plasma insulin, protected the pancreatic islet cells, and increased the expressions of GLUT4 and IRS-1, but decreased p-ERK expression in skeletal muscle and myocardial tissue. The results suggest that resveratrol or 17ß-estradiol shows obvious protection against STZ-induced diabetes in OVX mice, the mechanisms probably involve their ameliorating antioxidant activities and islet function, promoting muscle glucose uptake and inhibiting the expression of p-ERK.


Assuntos
Diabetes Mellitus Experimental/prevenção & controle , Estradiol/farmacologia , Ovariectomia , Resveratrol/farmacologia , Animais , Antioxidantes/metabolismo , Glicemia/metabolismo , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Relação Dose-Resposta a Droga , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Jejum/sangue , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Transportador de Glucose Tipo 4/genética , Transportador de Glucose Tipo 4/metabolismo , Insulina/sangue , Proteínas Substratos do Receptor de Insulina/metabolismo , Ilhotas Pancreáticas/efeitos dos fármacos , Ilhotas Pancreáticas/patologia , Malondialdeído/sangue , Camundongos , Fosfoproteínas/metabolismo
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