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1.
Cardiovasc J Afr ; 30: 1-11, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31361295

RESUMO

INTRODUCTION: Our previous experiments showed that the transient sodium current (INa) was abnormally increased in early ischaemia and atorvastatin could inhibit INa. The aim of this study was to observe the time-dependent effects of simulated ischaemia on INa and characterise the direct effects of atorvastatin on ischaemic INa. METHODS: Left ventricular myocytes were isolated from Wistar rats and randomly divided into two groups: a control group (normal to simulated ischaemia) and a statin group (normal to simulated ischaemia with 5 µmol/l atorvastatin). The INa was recorded under normal conditions (as baseline) by whole-cell patch clamp and recorded from three to 21 minutes in the next phase of simulated ischaemic conditions. RESULTS: In the control group, normalised INa (at -40 mV) was increased to the peak (1.15 ± 0.08 mA) at three minutes of ischaemia compared with baseline (0.95 ± 0.04 mA, p < 0.01), it subsequently returned to baseline levels at nine and 11 minutes of ischaemia (0.98 ± 0.12 and 0.92 ± 0.12 mA, respectively), and persistently decreased with prolonged ischaemic time. In the statin group, there were no differences between baseline and the early stages of ischaemia (0.97 ± 0.04 mA at baseline vs 0.92 ± 0.12 mA in ischaemia for three minutes, p > 0.05). CONCLUSION: Our results suggest that, in the early stages of ischaemia, changes in INa in ventricular myocytes are time-dependent, showing an initial increase followed by a decrease, while atorvastatin inhibited the transient increase in INa and made the change more gradual.

2.
Proc Natl Acad Sci U S A ; 116(22): 10937-10942, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31085638

RESUMO

Mirabegron (Myrbetriq) is a ß3-adrenoreceptor agonist approved for treating overactive bladder syndrome in human patients. This drug can activate brown adipose tissue (BAT) in adult humans and rodents through the ß3-adrenoreceptor-mediated sympathetic activation. However, the effect of the mirabegron, approved by the US Food and Drug Administration, on atherosclerosis-related cardiovascular disease is unknown. Here, we show that the clinical dose of mirabegron-induced BAT activation and browning of white adipose tissue (WAT) exacerbate atherosclerotic plaque development. In apolipoprotein E-/- (ApoE -/-) and low-density lipoprotein (LDL) receptor-/- (Ldlr -/-) mice, oral administration of clinically relevant doses of mirabegron markedly accelerates atherosclerotic plaque growth and instability by a mechanism of increasing plasma levels of both LDL-cholesterol and very LDL-cholesterol remnants. Stimulation of atherosclerotic plaque development by mirabegron is dependent on thermogenesis-triggered lipolysis. Genetic deletion of the critical thermogenesis-dependent protein, uncoupling protein 1, completely abrogates the mirabegron-induced atherosclerosis. Together, our findings suggest that mirabegron may trigger cardiovascular and cerebrovascular diseases in patients who suffer from atherosclerosis.

3.
Biosci Rep ; 39(5)2019 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-31064817

RESUMO

Background: Circulating microRNAs (miRNA) are steady preserved in blood plasma. Multiple evidences have shown that miRNAs play a crucial role in cardiovascular disease including miRNA-378, which has been illustrated to participate in diverse physiological and pathological processes of cardiovascular disease. In the present study, we aim to explore the expression of plasma miRNA-378 and its clinical significance in patients with coronary artery disease (CAD).Methods: MiRNA-378 expression in blood plasma was performed by quantitative real-time PCR (qRT-PCR) in 215 CAD patients and 52 matched controls of healthy populations. Medical information of all patients including the results of coronary angiography (CAG) was acquired through hospital information system (HIS). Spearman's correlation, binary linear regression, and covariance analysis were used to examine the association between miRNA-378 and relative clinical risk factors. Receiver operating characteristic curve analysis was applied to evaluate the value of miRNA-378 in predicting the disease severity of coronary lesion.Results: Plasma miR-378 expression was significantly down-regulated in CAD patients compared with healthy controls. Relative miR-378 level was shown conversely correlated with Gensini score, which present the severity of coronary artery lesions. Moreover, it is indicated that miR-378 expression can effectively distinguish patients with or without coronary artery stenosis.Conclusions: Plasma miR-378 levels appear to be a promising non-invasive biomarker, but require to be further validated by a large cohort study in future.

4.
BMC Med Genet ; 19(1): 185, 2018 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-30333000

RESUMO

BACKGROUND: Prostate cancer is a heterogeneous disease, meaning patients would benefit from different treatment strategies based on their molecular stratification. In recent years, several genomic studies have identified prostate cancers with defects in DNA repair genes. It is known that the PARP inhibitor, olaparib, has a significant synthetic lethal effect on tumors with BRCA 1/2 mutations, particularly in ovarian and breast cancer. CASE PRESENTATION: In this study, we describe a patient with metastatic castration-resistant prostate cancer (mCRPC) containing a BRCA2 germline mutation who underwent olaparib treatment. The efficacy of the treatment was monitored by serum TPSA level as well as mutation levels of circulating tumor DNA (ctDNA) using next-generation sequencing (NGS). The patient responded to the olaparib treatment as indicated by the minimal residual levels of TPSA and tumor-specific mutations of ctDNA in plasma after four months of treatment, although the patient eventually progressed at six-month post-treatment with significantly elevated and newly acquired somatic mutations in ctDNA. CONCLUSIONS: Our study provides evidence that mCRPC with BRCA2 germline mutations could response to PARP inhibitor, which improves patient's outcome. We further demonstrated that NGS-based genetic testing on liquid biopsy can be used to dynamically monitor the efficacy of treatment.

5.
Nat Mater ; 17(9): 800-807, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30061733

RESUMO

The spin-orbit torque (SOT) that arises from materials with large spin-orbit coupling promises a path for ultralow power and fast magnetic-based storage and computational devices. We investigated the SOT from magnetron-sputtered BixSe(1-x) thin films in BixSe(1-x)/Co20Fe60B20 heterostructures by using d.c. planar Hall and spin-torque ferromagnetic resonance (ST-FMR) methods. Remarkably, the spin torque efficiency (θS) was determined to be as large as 18.62 ± 0.13 and 8.67 ± 1.08 using the d.c. planar Hall and ST-FMR methods, respectively. Moreover, switching of the perpendicular CoFeB multilayers using the SOT from the BixSe(1-x) was observed at room temperature with a low critical magnetization switching current density of 4.3 × 105 A cm-2. Quantum transport simulations using a realistic sp3 tight-binding model suggests that the high SOT in sputtered BixSe(1-x) is due to the quantum confinement effect with a charge-to-spin conversion efficiency that enhances with reduced size and dimensionality. The demonstrated θS, ease of growth of the films on a silicon substrate and successful growth and switching of perpendicular CoFeB multilayers on BixSe(1-x) films provide an avenue for the use of BixSe(1-x) as a spin density generator in SOT-based memory and logic devices.

6.
ACS Appl Mater Interfaces ; 9(50): 43855-43860, 2017 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-29182245

RESUMO

To develop novel hole-transport materials (HTMs) with less synthetic steps is still a great challenge. Here, a small molecule hexakis[4-(N,N-di-p-methoxyphenylamino)phenyl]benzene (F-1) was successfully synthesized by a relatively simple scenario. F-1 exhibits a deep highest occupied molecular orbital energy level of -5.31 eV. Notably, F-1 also features 2 times higher hole mobility of 4.98 × 10-4 cm2 V-1 s-1 than that of the mostly used 2,2',7,7'-tetrakis(N,N-bis(4-methoxyphenyl)amino)-9,9'-spirobifluorene (spiro-OMeTAD). Consequently, F-1-based perovskite solar cells (PSCs) show markedly improved performance compared with spiro-OMeTAD-based ones. These results indicate such a material can be a promising HTM candidate to boost the overall performance of the PSC.

7.
Arch Oral Biol ; 60(11): 1613-7, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26351742

RESUMO

OBJECTIVE: Dental pulp stem cells (DPSCs) possess pluripotent properties that allow them to differentiate into multiple cell lineages, which can be potentially used in tissue regeneration. The aim of this in vitro study is to explore the effect of miRNAs on the myogenic differentiation of human adult DPSCs and seek for some potential biological factors for stable and feasible application in DPSC myogenic differentiation. METHODS: Human adult DPSCs were isolated from normal impacted third molars were treated with 5-Aza-2'-deoxycytidine to induce to myogenic differentiation in vitro. During this process the levels of myomiRNAs and myogenic marker genes were detected by real-time qPCR and Western blotting. Then antisense oligonucleotides of miR-143 and miR-135 were transfected into DPSCs to explore their effects on myogenic differentiation. Gene expression detection and MyHC immunofluorescence microscopy analysis were applied to characterize the myogenic differentiation of DPSCs. RESULTS: Expression of miR-135 and miR-143 was markedly decreased in myoblast DPSCs induced by 5-Aza. Part of the DPSCs treated with miR-135 or miR-143 inhibitors showed apparent myocytic properties and eventually fused to form myotubes. Co-transfection of miR-135 and miR-143 inhibitors impelled half of DPSCs to form myotubes. CONCLUSION: MiR-135 and miR-143 inhibitors could induce myogenic differentiation of DPSCs. Our findings indicated that miRNAs could exert a decisive function in induction of myogenic differentiation of DPSCs.


Assuntos
Células-Tronco Adultas/efeitos dos fármacos , Azacitidina/análogos & derivados , Polpa Dentária/efeitos dos fármacos , MicroRNAs/antagonistas & inibidores , Desenvolvimento Muscular/efeitos dos fármacos , Adulto , Células-Tronco Adultas/citologia , Células-Tronco Adultas/fisiologia , Azacitidina/farmacologia , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/genética , Células Cultivadas , Decitabina , Polpa Dentária/citologia , Polpa Dentária/metabolismo , Humanos , MicroRNAs/biossíntese , MicroRNAs/genética , Dente Serotino/citologia , Dente Serotino/efeitos dos fármacos , Desenvolvimento Muscular/genética , Músculo Esquelético/citologia , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/fisiologia , Mioblastos/citologia , Mioblastos/fisiologia , Oligonucleotídeos Antissenso/administração & dosagem , Oligonucleotídeos Antissenso/genética , Transfecção , Adulto Jovem
8.
Chin Med J (Engl) ; 128(14): 1948-55, 2015 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-26168837

RESUMO

BACKGROUND: The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. There is substantial literature on pain which has exactly effected on learning and memory; orthodontic tooth movement affected the emotional status has been showed positive outcomes. Danggui-Shaoyao-San (DSS) is a Traditional Chinese Medicine prescription that has been used for pain treatment and analgesic effect for orthodontic pain via inhibiting the activations of neuron and glia. We raised the hypothesis that DSS could restore the impaired abilities of spatial learning and memory via regulating neuron or glia expression in the hippocampus. METHODS: A total of 36 rats were randomly divided into three groups: (1) Sham group (n = 12), rats underwent all the operation procedure except for the placement of orthodontic forces and received saline treatment; (2) experimental tooth movement (ETM) group (n = 12), rats received saline treatment and ETM; (3) DSS + ETM (DETM) group (n = 12), rats received DSS treatment and ETM. All DETM group animals were administered with DSS at a dose of 150 mg/kg. Morris water maze test was evaluated; immunofluorescent histochemistry was used to identify astrocytes activation, and immunofluorescent dendritic spine analysis was used to identify the dendritic spines morphological characteristics expression levels in hippocampus. RESULTS: Maze training sessions during the 5 successive days revealed that ETM significantly deficits in progressive learning in rats, DSS that was given from day 5 prior to ETM enhanced progressive learning. The ETM group rats took longer to cross target quadrant during the probe trial and got less times to cross-platform than DETM group. The spine density in hippocampus in ETM group was significantly decreased compared to the sham group. In addition, thin and mature spine density were decreased too. However, the DSS administration could reverse the dendritic shrinkage and increase the spine density compared to the ETM group. Astrocytes activation showed the opposite trend in hippocampus dentate gyrus (DG). CONCLUSIONS: Treatment with DSS could restore the impaired abilities on ETM-induced decrease of learning and memory behavior. The decreased spines density in the hippocampus and astrocytes activation in DG of hippocampus in the ETM group rats may be related with the decline of the ability of learning and memory. The ability to change the synaptic plasticity in hippocampus after DSS administration may be correlated with the alleviation of impairment of learn and memory after ETM treatment.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Memória/efeitos dos fármacos , Aprendizagem Espacial/efeitos dos fármacos , Técnicas de Movimentação Dentária/efeitos adversos , Animais , Hipocampo/efeitos dos fármacos , Hipocampo/fisiologia , Masculino , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley
9.
Chin Med J (Engl) ; 127(20): 3630-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25316241

RESUMO

BACKGROUND: The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san (DSS) is a traditional Chinese medicine (TCM) prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia. METHODS: DSS was given twice a day from day 5 prior to experimental tooth movement (ETM). Directed face grooming and vacuous chewing movements (VCM) were evaluated. Immunofluorescent histochemistry and Western blot analysis were used to quantify the Iba-1 (microglia activation) and Fos (neuronal activation) expression levels in the trigeminal spinal nucleus caudalis (Vc). RESULTS: ETM significantly increased directed face grooming and VCM which reached the peak at post-operative day (POD) 1 and gradually decreased to the baseline at POD 7. However, a drastic peak increase of Fos expression in Vc was observed at 4 hours and gradually decreased to baseline at POD 7; while the increased Iba-1 level reached the peak at POD 1 and gradually decreased to baseline at POD 7. Furthermore, pre-treatment with DSS significantly attenuated the ETM induced directed face grooming and VCM as well as the Fos and Iba-1 levels at POD 1. CONCLUSION: Treatment with DSS had significant analgesic effects on ETM-induced pain, which was accompanied with inhibition of both neuronal and microglial activation.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Medicina Tradicional Chinesa/métodos , Microglia/efeitos dos fármacos , Manejo da Dor/métodos , Dor/tratamento farmacológico , Técnicas de Movimentação Dentária/efeitos adversos , Animais , Face/fisiologia , Masculino , Mastigação/fisiologia , Microglia/fisiologia , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Período Pós-Operatório , Ratos , Ratos Sprague-Dawley
10.
Neurosci Lett ; 568: 6-11, 2014 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-24686187

RESUMO

One promising strategy to prevent the chronicity of post-operative pain (POP) is to attenuate acute POP during the early phase by efficacious medications with fewer side effects. Duloxetine, one of the serotonin (5-HT)-norepinephrine (NE) reuptake inhibitors (SNRI), is used to treat a wide range of acute and chronic pain. However, its effect on POP has not been investigated. In the present study, we investigated the anti-hypersensitivity effect of duloxetine using a rat model of POP. The possible involvement of spinal 5-HT2A and α2-noradrenergic receptors were also evaluated by using antagonists for 5-HT2A (ketanserin) or α2-noradrenergic receptors (idazoxan). Finally, with the method of in vivo microdialysis, the increase in spinal NA and 5-HT levels after intraperitoneal (i.p.) delivery of duloxetine were investigated. The results showed that intrathecal (i.t.) or i.p. delivery of duloxetine produced an anti-hyperalgesic effect in a dose-dependent manner. The anti-hypersensitivity effect of duloxetine was partly attenuated by pretreatment with ketanserin or idazoxane. Microdialysis study revealed that 5-HT and NA concentrations at the spinal dorsal horn were increased, peaking at 30min after i.p. injection of 20mg/kg duloxetine. These findings indicate that duloxetine inhibits POP by increasing spinal NA and 5-HT levels and activating spinal 5-HT2A or α2-noradrenergic receptors.


Assuntos
Analgésicos/uso terapêutico , Dor Pós-Operatória/tratamento farmacológico , Inibidores de Captação de Serotonina/uso terapêutico , Tiofenos/uso terapêutico , Antagonistas de Receptores Adrenérgicos alfa 2/farmacologia , Analgésicos/administração & dosagem , Animais , Cloridrato de Duloxetina , Hiperalgesia/tratamento farmacológico , Hiperalgesia/metabolismo , Hiperalgesia/fisiopatologia , Idazoxano/farmacologia , Injeções Intraperitoneais , Injeções Espinhais , Ketanserina/farmacologia , Masculino , Microdiálise , Norepinefrina/metabolismo , Dor Pós-Operatória/metabolismo , Dor Pós-Operatória/fisiopatologia , Ratos Sprague-Dawley , Serotonina/metabolismo , Antagonistas do Receptor 5-HT2 de Serotonina/farmacologia , Inibidores de Captação de Serotonina/administração & dosagem , Medula Espinal/metabolismo , Tiofenos/administração & dosagem
11.
Brain Res Bull ; 81(6): 561-4, 2010 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-20067823

RESUMO

Cation chloride co-transporters, including K(+)-Cl(-) co-transporter 2 (KCC2) and Na(+)-K(+)-Cl(-) co-transporter 1 (NKCC1), are of particular importance to GABAergic transmission and thus involved in the development of hyperalgesia at the spinal level. However, it is largely unknown whether these co-transporters in the trigeminal system contribute to dental pain. In this study, we investigated the expression of KCC2 and NKCC1 mRNAs in mouse trigeminal subnucleus caudalis (Vc) after lipopolysaccharide (LPS) application to the tooth pulp by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method. KCC2 mRNA was found to be down-regulated at 1d after pulpal inflammation, while NKCC1 was up-regulated. Blockade of endogenous brain-derived neurotrophic factor-tyrosine receptor kinase B pathway with K252a produced pronounced antinociception as evidenced by decreased tongue protrusion behavior in LPS-treated mice. These data suggest that KCC2 and NKCC1 in Vc may play a critical role in the nociception and transmission of dental pain during pulpal inflammation.


Assuntos
Doenças da Polpa Dentária/metabolismo , Polpa Dentária/metabolismo , Inflamação/metabolismo , Simportadores de Cloreto de Sódio-Potássio/metabolismo , Simportadores/metabolismo , Núcleos do Trigêmeo/metabolismo , Analgésicos/farmacologia , Animais , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Carbazóis/farmacologia , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/imunologia , Doenças da Polpa Dentária/tratamento farmacológico , Doenças da Polpa Dentária/imunologia , Alcaloides Indólicos/farmacologia , Inflamação/tratamento farmacológico , Inflamação/imunologia , Lipopolissacarídeos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Dor/tratamento farmacológico , Dor/imunologia , Dor/metabolismo , RNA Mensageiro/metabolismo , Distribuição Aleatória , Receptor trkB/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais/efeitos dos fármacos , Membro 2 da Família 12 de Carreador de Soluto , Fatores de Tempo , Núcleos do Trigêmeo/efeitos dos fármacos
12.
J Clin Endocrinol Metab ; 90(5): 3045-53, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15705926

RESUMO

PL74, a novel member of the TGFbeta superfamily that has highest expression in placenta, is a multifunctional peptide that can induce differentiation, inhibit inflammatory stimulation of TNFalpha, and execute apoptosis after p53 overexpression and cytotoxic injury. To study its expression and function in placenta and preeclampsia, we first determined mRNA expression in nine normal and 10 preeclamptic placentas. PL74 mRNA was overexpressed by 57.3% in preeclampsia. Transfection of PL74 into term cytotrophoblasts resulted in increased apoptosis by terminal uridine deoxynucleotidyl nick end labeling labeling (control, 2.8 +/- 0.5%; PL74, 19.1 +/- 0.2%; P < 0.005). Addition of PL74 protein to HTR8/SVneo extravillous cytotrophoblast cells showed a dose-response (0-100 ng/ml) inhibition of [3H]thymidine uptake and increase in apoptosis shown by terminal uridine deoxynucleotidyl nick end labeling and histone-associated DNA fragment ELISA (control, 0.11 +/- 0.01 absorbance units; PL74, 0.21 +/- 0.01; P < 0.01). PL74 did not alter cytotrophoblast invasion using a Matrigel in vitro invasion assay. Cytokine regulation of PL74 mRNA expression in term cytotrophoblasts showed that epidermal growth factor and IFNgamma increased PL74 expression, but TGFbeta and TNFalpha had no effect. Transfection of antisense PL74 into term cytotrophoblast cells resulted in an inhibition of spontaneous differentiation at 2 and 24 h of culture (control vector, 30.8 +/- 3.1% and 26.4 +/- 1.2%; antisense PL74, 17.6 +/- 1.8%and 12.6 +/- 1.4% syncytial units, at 2 and 24 h respectively; P < 0.01). We conclude that PL74 is overexpressed in preeclampsia and may thus promote apoptosis of cytotrophoblasts at the expense of differentiation. PL74 secretion is induced by IFNgamma and may play a role in abnormal placental responses in preeclampsia.


Assuntos
Apoptose , Pré-Eclâmpsia/metabolismo , Fator de Crescimento Transformador beta/genética , Trofoblastos/patologia , Biópsia , Diferenciação Celular , Células Cultivadas , Feminino , Humanos , Interferon gama/farmacologia , Placenta/metabolismo , Placenta/patologia , Gravidez , RNA Mensageiro/análise , Fator de Crescimento Transformador beta/fisiologia , Fator de Necrose Tumoral alfa/farmacologia
13.
Hypertension ; 42(5): 895-900, 2003 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-14517225

RESUMO

To explore the mechanisms of adrenomedullin (ADM) regulation in normal and preeclamptic (PE) states, we determined placental production of ADM and ADM regulation by cytokines. Isolated, purified cytotrophoblast cultures from normal (n=8) and PE (n=10) placentas were cultured for 3 days in the absence or presence of 10 ng/mL epidermal growth factor (EGF), 1 ng/mL transforming growth factor (TGF)-beta1, 10 ng/mL tumor necrosis factor (TNF)-alpha, or 100 U/mL interferon (IFN)-gamma. Cells were also cultured for 3 days in 10% fetal bovine serum for determination of syncytial formation by desmoplakin staining. Pieces of normal and PE placentas were snap-frozen for ADM mRNA measurement. Results showed that basal ADM production into culture medium by radioimmunoassay was significantly lower in PE placental cells. EGF significantly stimulated ADM production in normal trophoblasts but did not in PE placentas. None of the factors TNF-alpha, TGF-beta1, or IFN-gamma altered ADM secretion in either normal or PE placentas. ADM expression by Northern blot analysis demonstrated a 34.3+/-8.3% reduction in mRNA expression in PE placentas. Syncytialization, as assessed by desmoplakin-outlined syncytial units, was decreased in PE placentas (day 3: normal, 16.7+/-1.3%; PE, 5.5+/-2.0%; P<0.01, ANOVA). However, there was a normal increment in syncytialization in response to EGF in normal and PE trophoblast preparations (EGF day 3: normal, 43.8+/-5.6%; PE, 46.1+/-12.3%). We conclude that spontaneous placental syncytialization is impaired in PE and that ADM production is markedly reduced in PE, possibly owing to an impaired EGF response. These abnormalities indicate poor placental production of ADM as the likely cause of a failed compensatory increase in maternal serum ADM levels in PE.


Assuntos
Fator de Crescimento Epidérmico/farmacologia , Peptídeos/metabolismo , Pré-Eclâmpsia/metabolismo , Adrenomedulina , Células Cultivadas , Feminino , Células Gigantes/citologia , Células Gigantes/metabolismo , Humanos , Peptídeos/genética , Pré-Eclâmpsia/diagnóstico , Pré-Eclâmpsia/genética , Gravidez , RNA Mensageiro/metabolismo , Trofoblastos/citologia , Trofoblastos/efeitos dos fármacos , Trofoblastos/metabolismo
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