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1.
J Colloid Interface Sci ; 577: 29-37, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32470702

RESUMO

Highly flexible supercapacitors (SCs) have attracted significant attention in modern electronics. However, it has been found that flexible, metal sulfide-based electrodes usually suffer from corrosion, instability and low conductivity, which significantly limits their large scale application. Herein, we report on an electrode comprised of highly stable, free-standing carbon fiber/trinickel disulphide covered with polyaniline (CF/Ni3S2@PANI). This electrode was prepared and then employed in a high-performance of flexible asymmetric SCs (FASC). The coating layer of polyaniline served as both a protector and conducting shell for the Ni3S2 due to the nature of the highly stable N-Ni bonds that formed between the polyaniline and Ni3S2. In addition, the lightweight carbon fiber support served as both a current collector and flexible support. The prepared CF/Ni3S2@PANI electrode exhibited a significantly enhanced specific capacity (715.3 F·g-1 at 1 A·g-1) compared with the carbon fiber/Ni3S2 electrode (318 F·g-1 at 1 A·g-1). More importantly, the assembled FASC device delivered an impressive energy density of 35.7 Wh·kg-1 at a power density of 850 W·kg-1. The FASC device benefited from the interconnected flexible microstructure and the stable bond bridges, so that it could be bent into various angles without noticeably impairing its performance. This effective protective strategy may further inspire the design and manufacture of metallic oxide or sulfide electrode with ultrahigh-stability interbond bridges for high-performance flexible supercapacitors.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 239: 118500, 2020 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-32470816

RESUMO

Diazinon is a typical phosphorothionate, which is widely used to prevent and control harmful organisms that endanger the agriculture productions. However, it is among the most toxic substances and can cause damage to the environment, food and human health even in very low concentrations. Hence, ultra-sensitive screening methods are urgently required for the detection of this extensively used pesticide. In this study, a rapid and sensitive fluorescence resonance energy transfer (FRET) method was developed for low concentration detection of diazinon in food. The aptamer-modified upconversion nanoparticles (Apt-UCNPs) were synthesized and conjugated with graphene oxide (GO) through π-π interaction. Due to the FRET between UCNPs and GO, the fluorescence was quenched. When diazinon was added, the aptamer preferentially bound with it, caused the separation of GO, and resulted in the enhancement of fluorescent signal. Under the optimal conditions, a wide linear detection range from 0.05 to 500 ng/mL was achieved, with a limit of detection (LOD) of 0.023 ng/mL. The proposed method was successfully applied to measure diazinon in real samples. Results showed that the proposed nanosensor offers an efficient, specific and simple approach for the detection of diazinon in food and has a high potential for food safety and quality control.

3.
Int J Mol Sci ; 21(9)2020 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-32354160

RESUMO

Abiotic stresses are major constraints limiting crop growth and production. Heat shock factors (Hsfs) play significant roles in mediating plant resistance to various environmental stresses, including heat, drought and salinity. In this study, we explored the biological functions and underlying mechanisms of wheat TaHsfA6f in plant tolerance to various abiotic stresses. Gene expression profiles showed that TaHsfA6f has relatively high expression levels in wheat leaves at the reproductive stage. Transcript levels of TaHsfA6f were substantially up-regulated by heat, dehydration, salinity, low temperature, and multiple phytohormones, but was not induced by brassinosteroids (BR). Subcellular localization analyses revealed that TaHsfA6f is localized to the nucleus. Overexpression of the TaHsfA6f gene in Arabidopsis results in improved tolerance to heat, drought and salt stresses, enhanced sensitivity to exogenous abscisic acid (ABA), and increased accumulation of ABA. Furthermore, RNA-sequencing data demonstrated that TaHsfA6f functions through up-regulation of a number of genes involved in ABA metabolism and signaling, and other stress-associated genes. Collectively, these results provide evidence that TaHsfA6f participates in the regulation of multiple abiotic stresses, and that TaHsfA6f could serve as a valuable gene for genetic modification of crop abiotic stress tolerance.

4.
Food Chem ; 323: 126812, 2020 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-32334303

RESUMO

The current study explores the first full mode liquid microextraction technique coupled with surface-enhanced Raman spectroscopy (SERS), and has been successfully applied for chromium speciation in food and environmental matrices. Herein, chromium as chlorochromate anion [CrO3Cl]- and the cationic rhodamine 6G [RG]+ dye has been extracted in organic phase as a complex ion associate [RG+.CrO3Cl-.nS]org at pH ≤ 1.0. Afterwards, the extracted phase was deposited on the surface of the nano-flower shaped silver nanoparticles substrate and the SERS response was monitored against the reagent blank at 1505 cm-1. Substrate characterizations, reaction mechanism assignment, stoichiometry, speciation, analytical applications, selectivity and validation were performed. The analytical procedure exhibits a detection limit of 0.03 µg L-1 under the optimized experimental conditions. The accuracy of the proposed strategy was validated by inductively coupled plasma optical emission spectrometry method using student's t- and F tests at 95% confidence.

5.
J Mol Graph Model ; 98: 107619, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32311663

RESUMO

Alpha-momorcharin (α-MMC), trichosanthin (TCS), and momordica anti-HIV protein of 30 kD (MAP30) are potential anti-tumor drug candidates but have cytotoxicity to normal cells. The binding of these proteins to LRP1 receptor and the subsequent endocytosis are essential to their cytotoxicity, but this binding process remains largely unknown. This study, in-silico analysis of the binding patterns, was conducted via the protein-protein docking software, ZDOCK 3.0.2 package, to better understand the binding process. Specifically, α-MMC, TCS and MAP30 were selected and bound to binding subunits CR56 and CR17 of LRP1. After docking, the 10 best docking solutions are retained based on the default ZDOCK scores and used for structural assessment. Our results showed that, α-MMC bound to LRP1 stably at the amino acid residues 1-20, at which 8 residues formed 21 hydrogen bonds with 15 residues of CR56 and 10 residues formed 15 hydrogen bonds with 12 residues of CR17. In contrast, TCS and MAP30 bound mainly to LRP1 at the residues 1-57/79-150 and residues 58-102, respectively, which were functional domains of TCS and MAP30. Since residues 1-20 are outside the functional domain of α-MMC, α-MMC is considered more suitable to attenuate by mutating the receptor binding site. Thus, our analysis lays the foundation for future genetic engineering work on α-MMC, and makes important contributions to its potential clinical use in cancer treatment.

6.
Biochem Biophys Res Commun ; 526(4): 993-998, 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32295713

RESUMO

Minichromosome maintenance 8 (MCM8) is a recently identified member of the minichromosome maintenance family, which possesses helicase and ATPase activity. It interacts with MCM9 and participates in homologous recombination repair. The structure of MCM8 is unclear now. Here, we report the crystal structure of the winged-helix domain of human MCM8 (MCM8-WHD) at 1.21 Å resolution. MCM8-WHD adopts a conserved winged-helix architecture. Structure analysis and biochemical study results showed the DNA binding ability and crucial residues of MCM8-WHD. Our results are helpful to understand the function of MCM8.

7.
Crit Rev Anal Chem ; : 1-28, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32233874

RESUMO

Noble bimetallic and trimetallic nanoparticles (NBT-NPs) have superior biomedical applications as compared to their monometallic counterparts. The performance of these nanomaterials depends on their composition, shape and size. Hence, the controlled-synthesis of these nanomaterials is a hot area of research. Till date, no review article in the literature accounts regarding the controlled-synthesis and biomedical applications related to morphology, optimum composition, biocompatibility and versatile chemistry of NBT-NPs. Taking this into contemplation, an effort was made to provide a clear insight into the morphology-controlled synthesis and size/shape-dependent anticancer and bactericidal applications of NBT-NPs. Chemical reduction method for the controlled-synthesis of NBT-NPs is reviewed critically. Furthermore, the potential role of various reaction parameters such as time, reducing agents, stabilizing/capping agents, nature/concentration of precursors, temperature and pH in the shape/size-controlled synthesis of these nanomaterials are discussed. In the second part of this article, anticancer and bactericidal applications of the NBT-NPs are reviewed and the influences of optimum composition, size, surface structure, versatile chemistry and synergism are studied. Finally, the current challenges in the controlled-synthesis and biomedical applications of these nanomaterials, and prospects to resolve related issues are discussed. HighlightsChemical reduction method for the synthesis of NBT-NPs is reviewed.The influences of parameters on the control synthesis of NBT-NPs are discussed.Antibacterial and anticancer applications and cytotoxicity of NBT-NPs are reviewed.Possible solutions for the key challenges are discussed.Outlooks about the synthesis and biomedical applications of NBT-NPs are discussed.

8.
Anal Chim Acta ; 1105: 45-55, 2020 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-32138925

RESUMO

Quantitative analysis of surface-enhanced Raman scattering (SERS) spectra has been a critical step in trace level analysis. In this study, a novel variable selection method called interval combination iterative optimization approach coupled with SIMPLS (ICIOA-SIMPLS) was proposed for simultaneously predicting the volume ratios of various pesticides by quantitative analysis of the SERS spectra of the compounds. Four strategies, including interval selection, model population analysis (MPA), weighted bootstrap sampling (WBS) and soft shrinkage were combined in the current designed ICIOA-SIMPLS approach. Firstly, the SERS spectra were split into a series of equal-width spectral intervals. Secondly, WBS, as a random sampling method was applied based on the initial weights of spectral intervals to generate random combinations of spectral intervals, namely sub-datasets. On this basis, multivariate calibration sub-models were developed by applying SIMPLS followed by MPA to statistically analyze the outputs of sub-models and update the weights of spectral intervals. Finally, using an iterative optimization procedure the optimal spectral interval combination with the lowest root mean squares error of cross-validation (RMSECV) was searched in a soft shrinkage manner. For the sake of investigating the performance of ICIOA-SIMPLS, four methods including SIMPLS, VCPA-SIMPLS, VISSA-SIMPLS and ICIOA-SIMPLS were tested on two groups of SERS spectra for comparison. The findings revealed that the best prediction performance was obtained with ICIOA-SIMPLS. Hence, this proposed method offers a robust and effective variable selection strategy for quantitative analysis of spectroscopic datasets.

9.
Int J Nurs Stud ; 105: 103554, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32200101

RESUMO

BACKGROUND: There is a shortage of nurses worldwide, and a large number of older nurses will be entering retirement in the near future. Older nurses have rich nursing experience, and their retirement is a major loss of resources for the nursing profession. Returning to work after retirement is becoming increasingly popular among retirees today, but there is limited knowledge of the perceptions of older nurses regarding continuing to work in a nursing career after retirement. OBJECTIVES: To explore older nurses' perceptions of continuing to work in a nursing career after retirement. DESIGN AND SETTINGS: We conducted a descriptive, qualitative study of older nurses from two public hospitals of different levels in China in 2018. PARTICIPANTS: A total of 27 older nurses aged 50-60 years were recruited, 15 from a tertiary hospital and 12 from a secondary hospital. METHODS: Individual semi-structured interviews were conducted with older nurses. An interview guide was developed to explore nurses' perceptions of continuing to work in a nursing career after retirement. Thematic analysis was used for the data analysis. We developed an interview guide and analysed the data based on the three essential conditions of the framework for understanding behaviour. FINDINGS: Based on the definition of the three essential conditions of the framework for understanding behaviour, 14 subthemes emerged from the data were categorised under this framework. The three main themes were as follows: the nurses' perceptions of their capability, motivation, and opportunity related to engaging in a nursing career after retirement. A number of older nurses with rich clinical experience have the ability and motivation to engage in nursing a career after retirement. However, discrimination against nurses, burnout, and the effects on young people's employment are barriers to older nurses considering working in a nursing career after retirement. Regarding re-employment after retirement, older nurses also expressed concerns about their physical health, family responsibilities, and salary as well as lack of managerial and policy support. CONCLUSIONS: These themes confirmed previous findings and offered new perspectives on older Chinese nurses' perceptions of working in a nursing career after retirement. These findings will influence future policy development and research directions. Policies for facilitating a prolonged working life should be developed. Future research should focus on how to address the challenges confronted by older nurses.

10.
J Cell Mol Med ; 24(8): 4762-4772, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32155686

RESUMO

Vascular smooth muscle cell (VSMC) proliferation is the pathological base of vascular remodelling diseases. Circular RNAs (circRNAs) are important regulators involved in various biological processes. However, the function of circRNAs in VSMC proliferation regulation remains largely unknown. This study was conducted to identify the key differentially expressed circRNAs (DEcircRNAs) and predict their functions in human aortic smooth muscle cell (HASMC) proliferation. To achieve this, DEcircRNAs between proliferative and quiescent HASMCs were detected using a microarray, followed by quantitative real-time RT-PCR validation. A DEcircRNA-miRNA-DEmRNA network was constructed, and functional annotation was performed using Gene Ontology (GO) and KEGG pathway analysis. The function of hsa_circ_0002579 in HASMC proliferation was analysed by Western blot. The functional annotation of the DEcircRNA-miRNA-DEmRNA network indicated that the four DEcircRNAs might play roles in the TGF-ß receptor signalling pathway, Ras signalling pathway, AMPK signalling pathway and Wnt signalling pathway. Twenty-seven DEcircRNAs with coding potential were screened. Hsa_circ_0002579 might be a pro-proliferation factor of HASMC. Overall, our study identified the key DEcircRNAs between proliferative and quiescent HASMCs, which might provide new important clues for exploring the functions of circRNAs in vascular remodelling diseases.

11.
Sci Rep ; 10(1): 4801, 2020 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-32179864

RESUMO

Agronomic characteristics and tolerance to biotic and abiotic stresses in hexaploid wheat can be drastically improved through wheat-alien introgression. However, the transcriptional level interactions of introduced alien genes in the wheat genetic background is rarely investigated. In this study, we report the genome-wide impacts of introgressed chromosomes derived from Ae. longissima on gene transcriptions of the wheat landrace Chinese Spring. RNA-seq analyses demonstrated 5.37% and 4.30% of the genes were significantly differentially expressed (DEGs) in CS-Ae. longissima disomic 3Sl#2(3B) substitution line TA3575 and disomic 6Sl#3 addition line TA7548, respectively when compared to CS. In addition, 561 DEGs, including 413 up-regulated and 148 down-regulated or not transcribed genes, were simultaneously impacted by introgressed chromosomes 3Sl#2 and 6Sl#3, which accounts for 41.25% of the DEGs in TA3575 and 38.79% in TA7548. Seventeen DEGs, annotated as R genes, were shared by both introgression lines carrying chromosomes 3Sl#2 and 6Sl#3, which confer resistance to powdery mildew. This study will benefit the understanding of the wheat gene responses as result of alien gene(s) or chromosome intogression and the plant defense response initiated by powdery mildew resistance genes in chromosomes 3Sl#2 and 6Sl#3.

12.
Chemosphere ; 248: 125927, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32014634

RESUMO

Composting is an acceptable and economically feasible process for recycling agricultural biomass waste. The addition of external additives to adjust the process of composting has been attracted lots of research attention. To investigate the effects of external additives on nutrients transformation process of composting, a laboratory reactors scale co-composting based on swine manure and corn straw (CK) with the additives of phosphate (MP), calcium bentonite (CB) and biochar (BC) were performed for 30 days. The results showed the addition of phosphate and biochar could contribute to accelerating temperature rise and shorten the thermophilic phase. The germination index (GI) of MP and BC achieved 180% and 150%, respectively. The excitation-emission matrix (EEM) demonstrated the intensities of the peak C (humic acids) of the MP treatment was 829.5, and the PV,n/PIII,n value (9.59) of MP treatment was particularly higher compared to other three treatments according to the fluorescence regional integration (FRI) analysis. The Fourier Transform Infrared spectroscopy (FTIR) indicated the rate of decomposition of aliphatic C substances was higher than that of aromatic C substances. According to the X-ray diffraction (XRD) spectra results, characteristic peaks at both 16° and 22° were decreased, indicating cellulose and amorphous components were degraded. It further proved the formation of struvite component in MP treatment. Therefore, based on the maturity indicators, EEM and XRD results, phosphate is an efficient additive and recommended for swine manure and corn straw co-composting.


Assuntos
Compostagem , Fosfatos/química , Bentonita/química , Carvão Vegetal/química , Germinação , Substâncias Húmicas , Esterco , Nitrogênio , Solo/química , Temperatura , Zea mays/química
13.
Chem Biodivers ; 17(4): e2000024, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32100940

RESUMO

Marine Micromonospora was revealed to be a rather untapped and a rich source of chemically diverse and unique bioactive natural products. This review is aimed to make a comprehensive survey of secondary metabolites that were derived from marine Micromonospora including chemical diversity and biological activities. A total of 116 compounds from 41 marine Micromonospora species have been reported, covering the literatures from 1997 to 2019. These compounds contain several structural classes such as polyketides (PKS), nonribosomal peptides (NRPS), PKS-NRPS hybrids, terpenes and others, and they present cytotoxic, antibacterial, antiparasitic, chemopreventive or antioxidant activities.

14.
Food Chem ; 315: 126300, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32018077

RESUMO

In this study, a novel sensor fabricated with compactly arranged gold nanoparticles (AuNPs) templated from mesoporous silica film (MSF) via air-water interface has been confirmed as a promising surface-enhanced Raman scattering (SERS) substrate for detecting trace levels of 2,4-dichlorophenoxyacetic acid (2,4-D), pymetrozine and thiamethoxam. The densely arranged AuNPs@MSF had an average AuNPs size of 5.15 nm with small nanogaps (<2nm) between AuNPs, and exhibited a high SERS performance. SERS spectra of pesticides were collected after their adsorption on the AuNPs@MSF. The results showed that the concentration of 2,4-D, pymetrozine and thiamethoxam gave a good linear relationship with SERS intensity. Moreover, the designed SERS-based sensor (AuNPs@MSF) was stable for 3 months with ca. 3% relative standard deviation (RSD) and was applied successfully for the analysis of 2,4-D extraction from both environmental and food samples. The proposed SERS-based sensor was further validated by HPLC and showed satisfactory result (p > 0.05).

15.
Phys Chem Chem Phys ; 22(7): 4023-4031, 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32022041

RESUMO

To improve the quality of chemical vapor deposition (CVD)-prepared hexagonal boron nitride (h-BN), it is essential to understand the growth mechanism, particularly to learn the structures as well as their stabilities and kinetic evolutions of the formed clusters in the initial growth stage. Herein, we performed systematic studies on the stabilities of various geometries of different-/identical-sized BN clusters on (111) surfaces of Ni and Cu by density functional theory simulations. The results show that the stable configurations of different-sized clusters are those containing the most normal hexagons composed with alternate B and N atoms. There exist ultra-stable magic clusters on the (111) surfaces of both the metals. On Ni(111), the geometries of the magic clusters are composed of hexagons arranged in the core-shell structure, while they contain tetragons on the Cu(111) surface. The ultra-high stabilities of the magic clusters can be attributed to the comprehensive effect from the core-shell structure, high symmetry, edged atoms, and adsorption sites. The stable geometries of different-sized clusters as well as magic clusters present the vital roles of metal substrates in CVD-synthesis of h-BN and provide instructive information in improving the quality of h-BN by selecting appropriate metal substrates.

16.
Theor Appl Genet ; 133(4): 1149-1159, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31932954

RESUMO

KEY MESSAGE: A spontaneous Robertsonian T4SlS·4BL translocation chromosome carrying Pm66 for powdery mildew resistance was discovered and confirmed by RNA-seq, molecular marker, and in situ hybridization analyses. Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a severe disease of bread wheat worldwide. Discovery and utilization of resistance genes to powdery mildew from wild relatives of wheat have played important roles in wheat improvement. Aegilops longissima, one of the S-genome diploid wild relatives of wheat, is a valuable source of disease and pest resistance for wheat. Chromosome 4Sl from Ae. longissima confers moderate resistance to powdery mildew. In this study, we conducted RNA-seq on a putative Chinese Spring (CS)-Ae. longissima 4Sl(4B) disomic substitution line (TA3465) to develop 4Sl-specific markers to assist the transfer of a Bgt resistance gene from 4Sl by induced homoeologous recombination. A pairwise comparison of genes between CS and TA3465 demonstrated that a number of genes on chromosome 4BS in CS were not expressed in TA3465. Analysis of 4B- and 4Sl-specific molecular markers showed that 4BS and 4SlL were both missing in TA3465, whereas 4BL and 4SlS were present. Further characterization by genomic and fluorescent in situ hybridization confirmed that TA3465 carried a spontaneous Robertsonian T4SlS·4BL translocation. Powdery mildew tests showed that TA3465 was resistant to 10 of 16 Bgt isolates collected from different regions of China, whereas CS was susceptible to all those Bgt isolates. The powdery mildew resistance gene(s) in TA3465 was further mapped to the short arm of 4Sl and designated as Pm66.

17.
Mikrochim Acta ; 187(1): 85, 2020 01 02.
Artigo em Inglês | MEDLINE | ID: mdl-31897844

RESUMO

A fluorescent nanoprobe for Pb(II) has been developed by employing aptamer-functionalized upconversion nanoparticles (UCNPs) and magnetic Fe3O4-modified (MNPs) gold nanoparticles (GNPs). First, aptamer-functionalized UCNPs and aptamer-functionalized magnetic GNPs were synthesized to obtained the fluorescent nanoprobe. The particles were combined by adding a complementary ssDNA. In the absence of Pb(II), the UCNPs, MNPs and GNPs are linked via complementary base pairing. This led to a decrease in the green upconversion fluorescence peaking at 547 nm (under 980 nm excitation). In the presence of Pb(II), the dsDNA between UCNPs and MNPs-GNPs is cleaved, and fluorescence recovers. This effect allows Pb(II) to be quantified, with a wide working range of 25-1400 nM and a lower detection limit of 5.7 nM. The nanoprobe gave satisfactory results when analyzing Pb(II) in tea and waste water. Graphical abstractSchematic representation of fluorescent nanoprobe based on fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (UCNPs) and gold nanoparticles (GNPs)-Fe3O4 magnetic nanoparticles (MNPs) for detection of Pb2+.

18.
J Cancer Res Clin Oncol ; 146(2): 367-379, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31953613

RESUMO

BACKGROUND: Long non-coding RNAs (lncRNAs) play crucial roles in the regulation and treatment of multiple myeloma (MM). The objective of this research was to study the functional mechanism of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in MM. METHODS: MALAT1, microRNA-1271-5p (miR-1271-5p), and SRY-Box 13 (SOX13) levels were examined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, apoptosis, and invasion were respectively assayed using 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT), flow cytometry, and transwell assay. Glycolysis was evaluated by glucose consumption, lactate production, ATP/ADP ratio, and the detection of related enzymes. Associated proteins were measured using Western blot. Target relation was verified via dual-luciferase reporter assay. Xenograft tumor assay was implemented to study the influence of MALAT1 on MM in vivo. RESULTS: The up-regulation of MALAT1 and the down-regulation of miR-1271-5p were found in MM serums and cells. MALAT1 knockdown suppressed cell viability, invasion, and glycolysis while expedited cell apoptosis in MM cells. MALAT1 directly targeted miR-1271-5p and miR-1271-5p depression reverted the effects of MALAT1 knockdown on MM cells. SOX13 was a target of miR-1271-5p and SOX13 overexpression weakened the effects of miR-1271-5p on MM. MALAT1 indirectly modulated SOX13 expression through targeting miR-1271-5p. MALAT1 down-regulation inhibited MM growth by miR-1271-5p/SOX13 axis in vivo. CONCLUSION: LncRNA MALAT1 expedited MM tumorigenesis, invasion, and glycolysis via miR-1271-5p/SOX13 axis. MALAT1 might contribute to the therapy of MM as a promising indicator.


Assuntos
Autoantígenos/metabolismo , MicroRNAs/metabolismo , Mieloma Múltiplo/metabolismo , RNA Longo não Codificante/metabolismo , Fatores de Transcrição SOXD/metabolismo , Animais , Autoantígenos/genética , Carcinogênese , Estudos de Casos e Controles , Linhagem Celular Tumoral , Glicólise , Xenoenxertos , Humanos , Camundongos , MicroRNAs/genética , Mieloma Múltiplo/sangue , Mieloma Múltiplo/genética , Mieloma Múltiplo/patologia , Invasividade Neoplásica , RNA Longo não Codificante/genética , Fatores de Transcrição SOXD/genética
19.
J Colloid Interface Sci ; 565: 503-512, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31982717

RESUMO

A one-dimensional hybrid with N,P co-doped carbon nanowires threaded CoP nanoparticles is rationally fabricated by employing surface modified coordination polymers as a precursor. Ultrasmall CoP nanoparticlesare well encapsulated in N,P co-doped carbon nanowires, which can effectively buffer the volume expansion of active CoP and facilitate fast lithium-ion/electron transfer during charge/discharge processes. Moreover, N,P co-doped carbon with high defect density and graphitic-N content are obtained, which facilitates high lithium storage capacity and fast electron transfer. As a result, attractive lithium storage properties are gained by employing this unique architecture as an anode material for lithium-ion batteries, including high reversible charge/discharge capacities, good rate capability, and excellent long-term cycling stability. Kinetic investigation shows that the fast lithium ion uptake/release is related to the remarkable capacitive contribution. This work may offer an effective way for design well-defined transition metal phosphide-based anodes for advanced lithium-ion batteries.

20.
Mater Sci Eng C Mater Biol Appl ; 108: 110421, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31923969

RESUMO

Phytochemicals sources have been extensively used as reducing and capping agents for synthesis of nanoparticles (NPs). However, morphology-controlled synthesis and shape/size dependent applications of these NPs still need to be explored further, and there is a need to develop a way in which particular and optimized phytochemicals result in the desired NPs in lesser time and cost with higher reproducibility rate. The present study is focused on morphology-controlled synthesis and shape/size dependent application of silver NPs based on the fractionated phytochemicals of Elaeagnus umbellata extract (EU). Unlike other approaches, in this study the reaction parameters such as time, temperature, pH, stirring speed and concentration of the precursor solutions were not altered during the optimization process. The fractionated phytochemicals were used separately for the synthesis of AgNPs, and the synthesized NPs were characterized by UV-visible, FT-IR, atomic force microscopy (AFM) and scanning electron microscopy (SEM). Our findings suggested that the constituents of the extract fractions varied with the selection of the extraction solvent, and the shape/size, bactericidal properties and toxicity of the NPs have a strong correlation with the phytochemicals of the plant extract. The fractionated phytochemicals present in the water fractions (EUW) resulted in monodispersed spherical AgNPs in the size about 40 nm. The NPs have significant stability in physiological conditions (i.e. temperature, pH and salt), have good antibacterial activity, and were found to be non-toxic. Furthermore, AFM and SEM analysis exposed that the NPs killed the bacteria by disturbing the cellular morphology and releasing the cellular matrix. Our results justify the use of different fractions of plant extract to obtain detail implications on shape, size, antibacterial potential and toxicity of AgNPs. This is the first step in a controllable, easy and cheap approach for the synthesis of highly stable, uniform, non-toxic and bactericidal AgNPs using five fractions of EU. The findings suggested that the synthesized NPs, particularly from EUW, could be used in pharmaceutical and homeopathic industry for the development of antibacterial medications.

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