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1.
J Med Food ; 21(9): 887-898, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30109956

RESUMO

Angelica sinensis (AS, Danggui in Chinese) is an important herbal component of various traditional formulae for the management of asthenia and its tonic effects. Although AS has been shown to ameliorate cognitive damage and nerve toxicity in D-galactose (D-gal)-elicited senescent mice brain, its effects on liver and kidney injury have not yet been explored. In this work, mice were subjected to hypodermic injection with D-gal (200 mg/kg) and orally gavaged with AS (20, 40, or 80 mg/kg) once a day for 8 successive weeks. Results revealed that AS significantly improved liver and kidney function as assessed by organ index and functional parameters. In addition, AS pretreatment effectively ameliorated the histological deterioration. AS attenuated the MDA level and markedly enhanced the activities and gene expressions of antioxidative enzymes, namely Cu, Zn-SOD, CAT, and GPx. Furthermore, AS markedly inhibited the D-gal-mediated increment of expressions of inflammatory cytokines iNOS, COX-2, IκBα, p-IκBα, and p65 and promoted the IκBα expression level in both hepatic and renal tissues. In sum, AS pretreatment could effectively guard the liver and kidney of mice from D-gal-induced injury, and the underlying mechanism was deemed to be intimately related to attenuating oxidative response and inflammatory stress.


Assuntos
Angelica sinensis/química , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Medicamentos de Ervas Chinesas/administração & dosagem , Galactose/efeitos adversos , Inflamação/tratamento farmacológico , Nefropatias/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Animais , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Cromatografia com Fluido Supercrítico , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Humanos , Inflamação/genética , Inflamação/metabolismo , Rim/efeitos dos fármacos , Nefropatias/induzido quimicamente , Nefropatias/metabolismo , Fígado/efeitos dos fármacos , Masculino , Malondialdeído/metabolismo , Camundongos
2.
PLoS One ; 13(3): e0194069, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29538417

RESUMO

Ulcerative colitis (UC) is a chronic relapsing disease without satisfactory treatments, in which intestinal inflammation and disrupted intestinal epithelial barrier are two main pathogeneses triggering UC. Berberrubine (BB) is deemed as one of the major active metabolite of berberine (BBR), a naturally-occurring isoquinoline alkaloid with appreciable anti-UC effect. This study aimed to comparatively investigate the therapeutic effects of BB and BBR on dextran sodium sulfate (DSS)-induced mouse colitis model, and explore the potential underlying mechanism. Results revealed that BB (20 mg/kg) produced a comparable therapeutic effect as BBR (50 mg/kg) and positive control sulfasalazine (200 mg/kg) by significantly reducing the disease activity index (DAI) with prolonged colon length and increased bodyweight as compared with the DSS group. BB treatment was shown to significantly ameliorate the DSS-induced colonic pathological alternations and decreased histological scores. In addition, BB markedly attenuated colonic inflammation by alleviating inflammatory cell infiltration and inhibiting myeloperoxidase (MPO) and cytokines (TNF-α, IFN-γ, IL-1ß, IL-6, IL-4 and IL-10) productions in DSS mice. Furthermore, BB treatment substantially upregulated the expression of tight junction (TJ) proteins (zonula occludens-1, zonula occludens-2, claudin-1, occludin) and mRNA expression of mucins (mucin-1 and mucin-2), and decreased the Bax/Bcl-2 ratio. In summary, BB exerted similar effect to its analogue BBR and positive control in attenuating DSS-induced UC with much lower dosage and similar mechanism. The protective effect observed may be intimately associated with maintaining the integrity of the intestinal mucosal barrier and mitigating intestinal inflammation, which were mediated at least partially, via favorable modulation of TJ proteins and mucins and inhibition of inflammatory mediators productions in the colonic tissue. This is the first report to demonstrate that BB possesses pronounced anti-UC effect similar to BBR and sulfasalazine with much smaller dosage. BB might have the potential to be further developed into a promising therapeutic option in the treatment of UC.


Assuntos
Berberina/análogos & derivados , Colite/tratamento farmacológico , Inflamação/tratamento farmacológico , Mucosa Intestinal/efeitos dos fármacos , Animais , Berberina/farmacologia , Colite/induzido quimicamente , Colite/metabolismo , Colo/efeitos dos fármacos , Colo/metabolismo , Citocinas/metabolismo , Sulfato de Dextrana/efeitos adversos , Modelos Animais de Doenças , Inflamação/induzido quimicamente , Inflamação/metabolismo , Mucosa Intestinal/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Peroxidase/metabolismo , Proteínas de Junções Íntimas/metabolismo , Junções Íntimas/metabolismo
3.
Sci China Life Sci ; 58(10): 1036-43, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26335730

RESUMO

We examined parenting stress and mental health status in parents of autistic children and assessed factors associated with such stress. Participants were parents of 188 autistic children diagnosed with DSM-IV criteria and parents of 144 normally developing children. Parents of autistic children reported higher levels of stress, depression, and anxiety than parents of normally developing children. Mothers of autistic children had a higher risk of depression and anxiety than that did parents of normally developing children. Mothers compared to fathers of autistic children were more vulnerable to depression. Age, behavior problems of autistic children, and mothers' anxiety were significantly associated with parenting stress.


Assuntos
Transtorno Autístico/fisiopatologia , Transtornos do Humor/psicologia , Pais/psicologia , Estresse Psicológico , Adulto , Criança , Feminino , Humanos , Masculino
4.
Artigo em Inglês | MEDLINE | ID: mdl-25910234

RESUMO

An ultra-high performance liquid chromatography tandem mass spectrometry (U-HPLC-MS/MS) method was developed and validated to determine liensinine and isoliensinine in rat plasma simultaneously. Plasma samples were prepared using protein precipitation with acetonitrile. The two analytes and the internal standard pirfenidone were separated on an Acquity U-HPLC BEH C18 column with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40mL/min. Both liensinine and isoliensinine were eluted at 0.63 and 0.82min, respectively. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 611.6 → 206.2 for liensinine and m/z 611.4 → 192.2 for isoliensinine. The linearity of this method was found to be within the concentration range of 5-700ng/mL for liensinine and isoliensinine in rat plasma. The lower limits of quantification (LLOQ) were all 5ng/mL for liensinine and isoliensinine. The relative standard deviations (RSD) of intra and inter precision were less than 10% for both liensinine and isoliensinine. The method was also successfully applied to the pharmacokinetic study of liensinine and isoliensinine in rats.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Isoquinolinas/sangue , Fenóis/sangue , Espectrometria de Massas em Tandem/métodos , Animais , Limite de Detecção , Masculino , Ratos , Ratos Wistar , Reprodutibilidade dos Testes
5.
Zhong Yao Cai ; 38(6): 1123-5, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26762049

RESUMO

OBJECTIVE: To establish ISSR-PCR system of cryopreservation regeneration plant of Gentiana straminea, and to select appropriate primers and analyze the genetic stability. METHODS: DNA was extracted by CTAB, the optimal ISSR-PCR system was established by orthogonal experiment,and genetic stability was analyzed. RESULTS: The optimal ISSR-PCR system (25 µL) was established: dNTPs 0.50 µL, Mg2+ 1.00 µL, 10 x PCR Buffer 2.00 µL, primer 0.60 µL, Taq DNA polymerase 1.25 µL, template DNA 1.30 µL, and ddH2O 18.35 µL. The amplification program was devised: 94 degrees C for 5 min, denaturing at 94 degrees C for 30 s, annealing of 1 min due to denaturing temperature of different primer,extension at 72 degrees C for 1.5 min, 35 cycles, last extension at 72 degrees C for 7 min, conservation at 4 degrees C . The DNA mutation rate of cryopreservation regeneration plant of Gentiana straminea was 1.05%. CONCLUSION: The cryopreservation regeneration plant of Gentiana straminea retains very good genetic stability, there is little variation between each plant, so the cryopreservation can be used as a feasible method for resource protection of Gentiana straminea.


Assuntos
Criopreservação , Gentiana/genética , Regeneração , Primers do DNA , DNA de Plantas , Instabilidade Genômica , Gentiana/crescimento & desenvolvimento , Reação em Cadeia da Polimerase
6.
Zhonghua Fu Chan Ke Za Zhi ; 46(7): 492-5, 2011 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22041439

RESUMO

OBJECTIVE: To investigate bone health conditions in 1637 aged women. METHODS: From May 2004 to October 2008, Bone mineral density (BMD) of 1637 women at age of more than 60 years old were measured by Hologic DephiA dual energy X-ray absorptiometry (DXEA) in Huadong hospital affiliated to Fudan University. All data were compared and analyzed among each group which will be divided by every ten years. Those women were divided into groups on 10 years range. BMD of lumbar vertebral and hip bone, fracture incidence and bone turnover marker were compared and analyzed. RESULTS: (1) BMD: at age of ≥90, 80-89, 70-79, 60-69, BMD of the lumbar vertebral 2-4 (L2-4) values were (0.96±0.18), (0.90±0.20), (0.81±0.16), (0.83±0.14) g/cm2, respectively. There were significantly increased BMD of lumbar of women at the age of 80-89 and ≥90 year-old compared with those of 60-69 year-old (P<0.05). At age of ≥90, 80-89, 70-79, 60-69 BMD of femur neck, Total, Torch, Ward's trianger were (0.60±0.11), (0.65±0.11), (0.47±0.09), (0.37±0.09) g/cm2; at age of 80-89 BMD of FN, Total, Torch, Ward's trianger were (0.57±0.10), (0.68±0.13), (0.48±0.11), (0.35±0.10) g/cm2; at age of 70-79 BMD of FN, Total, Torch, Ward's trianger were (0.57±0.10), (0.69±0.12), (0.49±0.10), (0.36±0.11) g/cm2; at age of 60-69 BMD of FN, Total, Torch, Ward's trianger were (0.63±0.10), (0.76±0.12), (0.54±0.10), (0.45±0.15) g/cm2; There were significantly decreased in BMD of hip at the age of 70-79, 80-89, ≥90 year-old compared with those of 60-69 year-old (P<0.05). (2) Fracture incidence:one time fracture incidence at age of 60-69, 70-79, 80-89, ≥90 were 34.8% (242/695), 45.0% (296/658), 51.3% (137/267), 5/17. There were increasing trend of fracture in aged women. (3) Bone turnover marker of bone Gla protein (BGP) N-mid (N-midBGP) in serum and C-terminal telopeptide of type I collagen/Cr (CTX/Cr) in urine values were (17±5) µg/L, (106±56) µg/mmol at age of more than 90 years, (17±7) µg/L, (128±99) µg/mmol at age of 80-89 years, (21±14) µg/L, (182±173) µg/mmol at age of 70-79 years, (25±13) µg/L, (190±168) µg/mmol at age of 60-69 years. There were significant decreased trends of N-midBGP at age of 70-79, 80-89 compared with that of 60-year (P<0.05). There were significant decreased trends of CTX/Cr 80-89 compared with that of 60-year (P<0.05). CONCLUSIONS: There were significant decreased bone metabolism in aged women. The risk of hip fracture is significantly increased in aged women. Diagnosis of osteoporosis based on BMD of hip in aged women is more reliable.


Assuntos
Idoso , Envelhecimento/fisiologia , Densidade Óssea/fisiologia , Nível de Saúde , Osteoporose/prevenção & controle , Absorciometria de Fóton , Fatores Etários , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Biomarcadores/urina , Osso e Ossos/metabolismo , Osso e Ossos/fisiopatologia , Colágeno Tipo I/urina , Feminino , Fraturas Ósseas/epidemiologia , Fraturas Ósseas/etiologia , Fraturas Ósseas/prevenção & controle , Humanos , Pessoa de Meia-Idade , Osteocalcina/sangue , Osteoporose/epidemiologia , Peptídeos/urina
7.
Huan Jing Ke Xue ; 29(4): 1045-52, 2008 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-18637360

RESUMO

The ammonia volatilization on the Typic Gleyi-stagnic Anthrosol with application of common urea and controlled release urea (LP-S100) fertilizers in the rice seasons in paddy soil of Taihui region of China was modeled by Jayaweera-Mikkelsen model. Results showed great difference of ammonia volatilization from two type fertilizers was detected with lysimeter experiment in the rice season. Nitrogen loss via ammonia volatilization after common urea application with conventional ways was 29%-35%, while only 5% of controlled release urea-N was volatilized. The Jayaweera-Mikkelsen model was over estimated the total amount of ammonia volatilization in the whole season, and great deviation from the measured data was obvious for the higher volatilization from common urea fertilizer. The estimated data were 2.95-4.19 times of the measures one for common urea treatments, while they were 1.19-1.40 times of those measured for LP-S100 treatments. The order of magnitude quotient was one of the indicators to evaluate the model estimation. The value of it was 0.8, which indicated the estimation of the model need improvement. Though sensitive analysis for the five parameters in the model was tested and amended the parameter of the concentration of NH4+ -N, a limited term was inducted in the model operation. The amended model got better results as the ratio of estimation to measured data was decreased to 1.12-1.28. The alga activity in the paddy field influenced ammonia volatilization and might make the failure of the model estimation of the original model.


Assuntos
Amônia/análise , Fertilizantes , Modelos Teóricos , Oryza/crescimento & desenvolvimento , Ureia/análise , Amônia/química , Ecossistema , Solo/análise , Ureia/química , Volatilização
8.
Zhonghua Yi Xue Za Zhi ; 88(6): 400-5, 2008 Feb 05.
Artigo em Chinês | MEDLINE | ID: mdl-18581895

RESUMO

OBJECTIVE: To examine the effects of monocyte chemoattractant protein-1 (MCP-1) on epithelial-myofibroblast transition (EMT) of renal proximal tubular epithelial cells and the possible mechanism involved. METHODS: Human renal proximal tubular epithelial cells of the line HK-2 were cultured and divided into three groups: negative control group; positive control group, treated with transforming growth factor (TGF)-beta1 5 microg/L, and MPC-1 group, treated with MCP-1 0.1, 1, 10, and 100 microg/L for 24 h, 36 h, 48 h, and 72 h respectively. The expressions of alpha-smooth muscle actin (alpha-SMA) mRNA and protein of cells were detected by s. Western blotting and RT-PCR were used to detect the expression of P-Erk1/2, Erk1/2, P-P38MAPK, P38MAPK, and RhoA protein levels of the HK-2 cells, and RT-PCR was used to detect the expression of RhoC and Snail mRNA. Specific inhibitors of the Erk, P38MAPK, and Rho signal transduction pathways PD98059, SB203580, and Y27632 were added into the culture fluid of HK-2 cells respectively, 1 h later MCP-1 microg/L was added for 48 h, and Western blotting was used to detect the protein expression of alpha-small muscle actin (SMA). RESULTS: The expression levels of alpha-SMA protein and mRNA significantly increased in the MCP-1 treated HK-2 cells,and these expression levels were the highest in the HK-2 cells treated with MCP-1 1 microg/L for 48 h. The ratios of (P-Erk1/2)/ (Erk1/2) and P-P38MAPK/P38MAPK were significantly increased (all P < 0.05) in the MCP-1 treated HK-2 cells with the highest ratios seen in the HK-2 cells treated by 100 microg/L of MCP-1. The expression levels of RhoA protein and RhoC mRNA of the HK-2 cells stimulated with MCP-1 of different concentrations were not significantly different (all P > 0.05). MCP-1 induced expression of a-SMA was only partly inhibited by PD98059 but not by SB203587 or Y27632. MCP-1 dose-dependently increased the expression of Snail mRNA of the HK-2 cells compared with those of the negative control cells. The level of Snail mRNA was the highest in the HK-2 cells treated with 100 microg/L MCP-1. CONCLUSION: MCP-1 may induce the EMT of renal proximal tubular epithelial cells in vitro, and this effect may involve upregulation of Erk1/2 Map kinase signal pathways and Snail mRNA expression. P38MAPK or Rho kinase signal pathways can not be proven to be involved in MCP-1 induced EMT.


Assuntos
Transdiferenciação Celular/efeitos dos fármacos , Quimiocina CCL2/farmacologia , Células Epiteliais/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Actinas/genética , Actinas/metabolismo , Western Blotting , Linhagem Celular , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Fibroblastos/citologia , Fibroblastos/metabolismo , Humanos , Túbulos Renais/citologia , Células Musculares/citologia , Células Musculares/efeitos dos fármacos , Células Musculares/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa
9.
Phytochem Anal ; 19(2): 141-7, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-17853383

RESUMO

The stability of diester-diterpenoid alkaloids (DDA) from plants of the genus Aconitum L. has been studied in different solvents and pH buffers. The HPLC/ESIMS method for analysing the concentration of DDA was established and DDA's decomposition products were elucidated by HPLC/ESI-MS/MS(n). In different solvents, e.g. dichloromethane, ether, methanol and distilled water, the decomposition pathways of DDA are quite different and their difference in stabilities depends on the difference of their structures, in which substituents at the N atom and substituents at C-3 are different. The pyrolytic products of DDA, such as deacetoxy aconitine-type alkaloids, have been observed in the above solvents, whereas 8-methoxy-14-benzoyl aconitine-type alkaloids have been obtained only in methanol. Furthermore, the experimental results demonstrate that the stability of DDA depends on pH values of the buffer. Aconine as hydrolysate has been only found in pH 10.0 buffer, and the other hydrolysates and the pyrolyzates of DDA, such as benzoylaconine and deacetoxy aconitine, have been observed in all pH aqueous solutions. The decomposition pathways of DDA in buffers are related to the substituent on the C-3 position. The decomposition pathway of aconitine is similar to that of mesaconitine, but different from that of hypaconitine.


Assuntos
Aconitum/química , Alcaloides/análise , Alcaloides/química , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Éter/química , Cloreto de Metileno/química , Estrutura Molecular , Solventes/química , Água/química
10.
Clin Sci (Lond) ; 108(6): 547-52, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15707387

RESUMO

The present study was designed to investigate the effects of low-dose ketanserin on BPV (blood pressure variability), BRS (baroreflex sensitivity) and organ damage in SHR (spontaneously hypertensive rats). Ketanserin was mixed in rat chow at an estimated dose of 0.1 mg.kg(-1) of body weight.day(-1). SHR were treated for 4 months. BP (blood pressure) was then recorded continuously for 24 h in a conscious state. After determination of BRS, rats were killed for organ damage evaluation. It was found that long-term treatment with low-dose ketanserin did not lower BP levels, but significantly decreased BPV, enhanced BRS and reduced organ damage in SHR. Multiple regression analysis showed that the decrease in left ventricular hypertrophy was most closely correlated (or associated) with the increase in BRS, whereas the decrease in aortic hypertrophy was most closely associated with the decrease in diastolic BPV and the amelioration in renal lesion, with the increase in BRS and the decrease in diastolic BPV. In conclusion, low-dose ketanserin produces organ protection independently of its BP-lowering action in SHR, and this organ protection was importantly attributable to the enhancement of BRS and decrease in BPV.


Assuntos
Barorreflexo/efeitos dos fármacos , Pressão Sanguínea/efeitos dos fármacos , Ketanserina/farmacologia , Antagonistas da Serotonina/farmacologia , Animais , Aorta/efeitos dos fármacos , Diástole , Coração/efeitos dos fármacos , Hipertrofia , Rim/efeitos dos fármacos , Masculino , Ratos , Ratos Endogâmicos SHR
11.
Zhonghua Fu Chan Ke Za Zhi ; 40(12): 796-8, 2005 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-16412321

RESUMO

OBJECTIVE: To evaluate the relationship between osteoporosis (OP) and the age of menarche and menopause, parity and lactation and bone mineral density (BMD). METHODS: BMD was measured in 1472 postmenopausal women by Norland XR-36 dual energy X-ray absorptiometry (DXA) from May 1999 to April 2003 in our hospital. All data of BMD were compared and statistically analyzed between women with different age of menarche and menopause, parity and lactation period. The diagnostic criteria of osteoporosis was defined by a loss of BMD greater than 2.5 standard deviations below average peak BMD of young adult women. RESULTS: In all 1472 subjects, the incidence of osteoporosis is 58.5% (861/1472). There was significant increase in osteoporosis of lumbar spine in women with the age of menarche > or = 17 (119 out of 336 cases were with osteoporosis in lumbar) compared with women with age of menarche < or = 13 (75 out of 276 cases were with osteoporosis in lumbar). In women with menarche age of 11-13, 14-16, 17-19, lumbar vertebral (L(2-4)) BMD values were (0.83 +/- 0.16), (0.82 +/- 0.16), (0.80 +/- 0.14) g/cm(2), respectively. There was significant increase in BMD of lumbar in the menarche age group of 11-13 compared with the age group of 17-19 in women within menopause 1-10 years (P < 0.05). Among all women aged between 55-65, there was significant increase in OP in women with the menopause age < or = 48 compared with menopause age > or = 54 (P < 0.01). BMD of the L(2-4), Trochanter, Ward triangle was significantly increased in women who had had one delivery or being nulliparous compared with women having more than four deliveries; BMD of the L(2-4), Ward's triangle were significantly increased in women with less than six months of lactation compared with those lactating more than thirty-six months. CONCLUSIONS: The later the menarche and the earlier the menopause, the higher the degree of osteoporosis; the more deliveries and period of lactation, the lower the BMD. Age is one of the high risk factors of osteoporosis.


Assuntos
Menarca , Osteoporose Pós-Menopausa/epidemiologia , Adolescente , Adulto , Fatores Etários , Idoso , Densidade Óssea , Criança , Feminino , Humanos , Pessoa de Meia-Idade
12.
Chin Med J (Engl) ; 117(7): 1029-35, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15265377

RESUMO

BACKGROUND: Raloxifene has been approved for prevention and treatment of postmenopausal osteoporosis in Caucasian women. It also has some positive effects on serum lipids in Caucasians. The objective of this study was to determine the effect of raloxifene hydrochloride on lumbar spine and total hip bone mineral density (BMD), bone metabolism, and serum lipids in Chinese postmenopausal women with osteoporosis. METHODS: This was a multi-center, randomized, double-blind, placebo-controlled clinical trial in which 204 postmenopausal Chinese women with osteoporosis were assigned to receive raloxifene (60 mg) or placebo treatment daily for 12 months. BMD, serum bone metabolism markers, and serum lipids were measured before and after drug administration. BMD was measured by Dual-Energy X-Ray Absorptiometry (DEXA) and bone metabolism markers were analyzed by one-step enzyme-linked immunosorbent assay. Serum lipids were measured by enzymatic analysis. RESULTS: At the end of the 12-month study, lumbar spine BMD increased in both groups with a mean increase of (3.3 +/- 4.8)% in the raloxifene group and (1.0 +/- 4.9)% in the placebo group (P < 0.001). There was a mean increase in total hip BMD of (1.4 +/- 4.8)% in the raloxifene group and a mean decrease of (0.9 +/- 5.0)% in the placebo group (P < 0.001). No subject in the raloxifene group had a new vertebral fracture and 5 placebo subjects had new fractures (P > 0.05). In the raloxifene group, the median decreases in the biochemical markers of bone metabolism serum osteocalcin and C-telopeptide were 41.7% and 61.5%, respectively. These changes were statistically significant compared with those in the placebo group (10.6% and 35.6%, P < 0.001, respectively). Both total cholesterol and low-density lipoprotein cholesterol decreased significantly in the raloxifene group compared with those in the placebo group (P < 0.001, respectively) and there was no significant effect of raloxifene on high-density lipoprotein cholesterol and triglycerides compared with placebo. CONCLUSIONS: Raloxifene 60 mg/d for 12 months significantly increases lumbar spine and total hip BMD, significantly decreases bone turnover, and has favourable effects on serum lipids in Chinese postmenopausal women with osteoporosis.


Assuntos
Densidade Óssea/efeitos dos fármacos , Osso e Ossos/metabolismo , Lipídeos/sangue , Osteoporose Pós-Menopausa/tratamento farmacológico , Cloridrato de Raloxifeno/uso terapêutico , Idoso , Idoso de 80 Anos ou mais , Osso e Ossos/efeitos dos fármacos , Feminino , Humanos , Pessoa de Meia-Idade , Cloridrato de Raloxifeno/efeitos adversos
13.
Zhonghua Yi Xue Za Zhi ; 84(4): 269-73, 2004 Feb 17.
Artigo em Chinês | MEDLINE | ID: mdl-15059505

RESUMO

OBJECTIVE: To determine the effect of raloxifene hydrochloride (RLX) on the lumbar spine and total hip bone mineral density (BMD), bone metabolism and serum lipids in Chinese postmenopausal women with osteoporosis. METHODS: 204 Chinese postmenopausal women with osteoporosis from 3 hospitals in Beijing and Shanghai were randomly divided into 2 groups of 102 women: RLX group (RLX of the dosage of 60 mg/day was given for 12 months) and placebo group. In addition, 500 mg of elemental calcium and 200 units of vitamin D were given daily to all women. BMD, serum bone markers and lipids were measured before and after drug administration. The BMD of lumber spine and hip was measured by dual-energy X-ray absorptiometry (DEXA). Serum bone gamma-carboxyglutamic acid-containing protein (BGP) and C-teloppeptide were analyzed by one-step ELISA. Serum lipids were measured by enzymatic method. RESULTS: By the end of the 12-month study period, the lumbar spine BMD was increased by 3.3% +/- 4.8% in the RLX group and 1.0% +/- 4.9% in the placebo group (P < 0.001); the hip BMD was increased by 1.4% +/- 4.8%in the RLX group and decreased by 0.9% +/- 5.0% in the placebo group (P < 0.01). New vertebral fracture occurred in none of the subjects in the RLX group and in 5 subjects of the placebo group (P = 0.059). The serum BGP and CTX decreased by 41.7% and 61.5% respectively in the RLX group, both significantly more than those in the placebo group (10.6% and 35.6% respectively, both P < 0.001). Both the total cholesterol and low-density lipoprotein cholesterol were significantly lower in the RLX group than in the placebo group (both P < 0.001), however, there were no significant differences in high-density lipoprotein cholesterol and triglycerides between these two groups. One subject in the RLX group and 5 subjects in the placebo group discontinued the study due to adverse events. There were no differences in the number of subjects with hot flushes (3 in the RLX group and 1 in the placebo group) and the number of subjects with leg cramps (9 in the RLX group and 4 in the placebo group). No venous thromboembolic event was reported. CONCLUSION: RLX of the dosage of 60 mg/day for 12 months significantly increases the lumbar spine and total hip bone BMD, significantly decreases bone turnover and has favorable effects on serum lipids in Chinese postmenopausal women with osteoporosis.


Assuntos
Densidade Óssea/efeitos dos fármacos , Antagonistas de Estrogênios/uso terapêutico , Osteoporose Pós-Menopausa/tratamento farmacológico , Cloridrato de Raloxifeno/uso terapêutico , Ácido 1-Carboxiglutâmico/sangue , Absorciometria de Fóton , Idoso , Colágeno/sangue , Colágeno Tipo I , Método Duplo-Cego , Ensaio de Imunoadsorção Enzimática , Antagonistas de Estrogênios/efeitos adversos , Feminino , Humanos , Lipídeos/sangue , Vértebras Lombares/efeitos dos fármacos , Vértebras Lombares/metabolismo , Pessoa de Meia-Idade , Osteoporose Pós-Menopausa/metabolismo , Ossos Pélvicos/efeitos dos fármacos , Ossos Pélvicos/metabolismo , Peptídeos/sangue , Cloridrato de Raloxifeno/efeitos adversos , Resultado do Tratamento
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