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1.
J Clin Lab Anal ; : e23014, 2019 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-31444844

RESUMO

BACKGROUND: Light-initiated chemiluminescent assays (LICA) are homogeneous assays that are sensitive, specific, and free of separation and washing steps and have high throughput and high precision. METHODS: In this research, we developed a competitive method by LICA to achieve accurate quantification of estradiol (E2) in human serum. E2 competed with estriol (E3) for binding to anti-human E2 antibodies. E3 was linked to biotin via bovine serum albumin as a linker. As this assay used competition between the labeled tracer and the analyte, an increase in E2 concentration will cause a signal decrease. RESULTS: The expected detection range of E2 was 20-5000 pg/mL. The analytical and functional sensitivities were 7.16 and 13.7 pg/mL, respectively. The intra- and inter-assay coefficients of variation were both below 15%, and the recovery rate ranged from 97.5% to 106.8%. The interference rates ranged from -3.6% to 5.4% and met detection requirements for E2 in hyperbilirubinemia, hemolysis, and lipemia in clinical samples. In addition, the cross-reactivity rates between E2 and structural analogs and some reproductive hormones varied from 1.9% to 10.6% which showed that LICA is highly specific for E2. Moreover, our results showed high accordance with the IMMULITE 2000 (y = 0.6695x + 47.92, r2  = .843) and VIDAS systems (y = 1.099x - 821.5, r2  = .9392). CONCLUSION: Our data show that the LICA, which is easy to automate, is a promising technique for quantification of E2 in human serum and could be used for clinical detection.

2.
Mol Plant ; 12(7): 984-1002, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31059824

RESUMO

During embryo development, the vascular precursors and ground tissue stem cells divide to renew themselves and produce the vascular tissue, endodermal cells, and cortical cells. However, the molecular mechanisms regulating division of these stem cells have remained largely elusive. In this study, we show that loss of function of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) genes results in aberrant embryo development. Fewer cortical, endodermal, and vascular cells are generated in the embryos of serk1 serk2 bak1 triple mutants. WUSCHEL-RELATED HOMEOBOX 5 (WOX5) is ectopically expressed in vascular cells of serk1 serk2 bak1 embryos. The first transverse division of vascular precursors in mid-globular embryos and second asymmetric division of ground tissue stem cells in early-heart embryos are abnormally altered to a longitudinal division. The embryo defects can be partially rescued by constitutively activated mitogen-activated protein kinase (MAPK) kinase kinase YODA (YDA) and MAPK kinase MKK5. Taken together, our results reveal that SERK-mediated signals regulate division patterns of vascular precursors and ground tissue stem cells, likely via the YDA-MKK4/5 cascade, during embryo development.

3.
Ann Clin Lab Sci ; 49(1): 57-62, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30814078

RESUMO

OBJECTIVES: Serum 14-3-3η is a novel joint-derived proinflammatory mediator associated with rheumatoid arthritis (RA). This study aimed to evaluate the diagnostic capacity of serum 14-3-3η and its correlation with clinical variables in patients with RA. METHODS: A total of 94 patients with RA and 80 age- and sex-matched controls, including 40 healthy subjects, were included. Serum 14-3-3η levels were assessed by quantitative enzyme-linked immunosorbent assay. Receiver-operating characteristic (ROC) curves analysis was used to determine the sensitivity and specificity of 14-3-3η. Spearman's rank correlation coefficient was used to assess the relationship between 14-3-3η and other clinical measures in patients with RA. RESULTS: Median (interquartile range) of serum 14-3-3η concentration (ng/ml) in RA patients (2.34 [1.56-3.39]) was significantly higher than that in healthy subjects (0.17[0.11-0.30]) and disease controls (1.66[1.21-2.74]; P<0.05). ROC curve analysis comparing patients with RA with all controls demonstrated a significant (P<0.001) area under the curve (AUC) of 0.81 (95% confidence interval: 0.74-0.88). At a cutoff of 1.44 ng/mL, the ROC curve yielded a sensitivity of 78.7% and specificity of 73.8%. The sensitivity of anti-citrullinated protein antibody (ACPA) and rheumatoid factor (RF) were 84.0% and 72.3%, respectively. Adding 14-3-3η to ACPA and/or RF discriminated more than 96% of patients with RA. The positive rate of at least one of the three markers was up to 99%, with a specificity of about 70%. The results of correlation analyses revealed that serum levels of 14-3-3η protein positively correlated with C-reactive protein (r=0.250, P<0.05), erythrocyte sedimentation rate (r=0.294, P<0.01), and 28-joint disease activity score (r=0.275, P<0.05) in patients with RA. CONCLUSIONS: 14-3-3η protein is a novel marker that can apparently enhance the detection rate of patients with RA. The level of serum 14-3-3η protein correlates to some degree with disease activity.


Assuntos
Proteínas 14-3-3/sangue , Artrite Reumatoide/sangue , Artrite Reumatoide/diagnóstico , Biomarcadores/sangue , Índice de Gravidade de Doença , Estudos de Casos e Controles , Progressão da Doença , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC
4.
Clin Chim Acta ; 2018 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-30517849

RESUMO

BACKGROUND: An increase in allergen-specific IgG4 (sIgG4), which serves as a blocking antibody, is associated with acquisition of immune tolerance after immunotherapy. In this study, we developed a rapid, sensitive, and homogeneous immunoassay based on the light-initiated chemiluminescent assay (LICA) technology for quantifying allergen sIgG4 in serum samples. METHODS: Allergen sIgG4 was measured in vitro by incubating the sample with biotinylated allergens and chemiluminescent beads coated with anti-human IgG4 antibody, followed by the addition of streptavidin-coated sensitizer beads. Multiple tests were performed to optimize the working conditions of the LICA and evaluate its performance. RESULTS: We established the optimal concentration of biotinylated allergens (250 ng/mL), the optimal dilution range (1:8 for Gal d 1, Gal d 2 sIgG4 and 1:4 for Gal d 3, Gal d 4 sIgG4), and the optimal incubation time (20 min for Gal d 1, Gal d 2 sIgG4 and 40 min for Gal d 3, Gal d 4 sIgG4). The lower limit of quantification (LLOQ) was 0.261 ng/mL. The coefficient variation (CV) of the LICA was <10%. The assay was unaffected by general interfering substances at physiological concentrations. It exhibited excellent accuracy to detect allergen-sIgG4 in human serum. Additionally, we demonstrated that the levels of Gal d 1, Gal d 2, and Gal d 3-sIgG4 were significantly higher in the egg allergy group (p < .05), but no differences were found between the groups for Gal d 4-sIgG4. CONCLUSIONS: The LICA demonstrated satisfactory performance and can be used for quantifying allergen sIgG4 in clinical practice.

5.
Anal Bioanal Chem ; 2018 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-30478516

RESUMO

This paper described a homogeneous method, light-initiated chemiluminescent assay (LICA), for quantitation of total testosterone in human sera. The assay was bead based and built on a competitive-binding reaction format, in which 5-α-dihydrotestosterone (5-α-DHT) competed with the testosterone in serum samples in binding with biotinylated anti-testosterone antibody. The more testosterone in the serum sample, the less 5-α-DHT that bonded with biotinylated anti-testosterone antibodies. 5-α-DHT was coupled with emission beads (doped with thioxene derivatives and Eu(III) as a chemiluminescence emitter) via bovine serum albumin as a linker. Once streptavidin-coated sensitizer beads (modified with phthalocyanine as a photosensitizer) were added, the streptavidin/biotin reaction between 5-α-DHT-bound anti-testosterone antibody and sensitizer beads could bring emission and sensitizer beads together, which allowed energy transfer from sensitizer bead to emission bead. As such, an exciting light (680 nm) impinging on the sensitizer beads led to light emission at 520-620 nm by emission beads. The strength of the emitted light was inversely proportional to the testosterone in serum sample. The detection range of this assay was from 13.3 to 1200 ng/dL. The coefficient variation for intra- and inter-assay was lower than 15%. The recovery of this method ranged from 95.5 to 105.9% for different samples. Moreover, the LICA assay was highly specific with low cross-reactivity and interference. The concentration of testosterone from 58 serum samples analyzed by the LICA method significantly correlated (y = 0.97x + 1.87, R2 = 0.970, p < 0.001) with those obtained with the SIEMENS Centaur Xp System. Graphical abstract ᅟ.

6.
Water Environ Res ; 90(11): 1977-1984, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30486925

RESUMO

A lab-scale aerobic-anoxic-aerobic (AE1-AN-AE2) MBBR system was tested for the removal of COD, , SCN-, phenols, and nitrogen from coal gasification wastewater, using a shortcut biological nitrogen removal process. Dissolved oxygen concentration in AE1 was maintained at 1.0 to 2.0 mg/L to ensure stable accumulation. Adding methanol wastewater to AN guaranteed denitrification efficiency. AE2 ensured high removal rates of , SCN-, and phenols. The effects of influent pollutant concentration and hydraulic retention time (HRT) on nitrogen removal were studied. Improving the dissolved oxygen concentration in AE1 eliminated the negative effect of increased organic loading on nitrification, but it affected the stability of nitrosation. Shortening the HRT had negative effects on the performance of the system and performance recovered after it was extended. The average total nitrogen removal rate was 82.6% with a CODmethanol/ ratio of 3.5. Biomass and activity of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria were measured to understand the evolution of nitrification.

7.
Sci Adv ; 4(11): eaau8408, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30474060

RESUMO

A trimer-of-hairpins motif has been identified in triggering virus-cell fusion within a variety of viral envelopes. Chemically manipulating such a motif represents current repertoire of viral fusion inhibitors. Here, we report that triterpenoids, a class of natural products, antagonize this trimer-of-hairpins via its constitutive heptad repeat-2 (HR2), a prevalent α-helical coil in class I viral fusion proteins. Triterpenoids inhibit the entry of Ebola, Marburg, HIV, and influenza A viruses with distinct structure-activity relationships. Specifically, triterpenoid probes capture the viral envelope via photocrosslinking HR2. Profiling the Ebola HR2-triterpenoid interactions using amino acid substitution, surface plasmon resonance, and nuclear magnetic resonance revealed six residues accessible to triterpenoids, leading to wrapping of the hydrophobic helix and blocking of the HR1-HR2 interaction critical in the trimer-of-hairpins formation. This finding was also observed in the envelopes of HIV and influenza A viruses and might potentially extend to a broader variety of viruses, providing a mechanistic insight into triterpenoid-mediated modulation of viral fusion.

8.
Int Immunopharmacol ; 61: 126-131, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29859469

RESUMO

BACKGROUND: Increasing dairy consumption in China has been accompanied by rising incidence of milk allergy. Here we analyzed profiles of specific immunoglobulin E (sIgE) against cow's milk proteins, and assessed their value for milk allergy diagnosis among infants and young children from northern China. METHODS: Sera collected from 48 patients with milk allergy and 27 negative control subjects was analyzed by enzyme-linked immunosorbent assay to measure sIgE to α-lactalbumin (Bos d 4), ß-lactoglobulin (Bos d 5), α-casein (Bos d 9), ß-casein (Bos d 11), and κ-casein (Bos d 12). RESULTS: Among milk-allergic individuals, most were sensitized to at least one milk protein; about half were sensitized to Bos d 5, Bos d 9, Bos d 11 and Bos d 12, respectively, while few had positive serum sIgE against Bos d 4. Bos d 12 sIgE had the largest area under curve (AUC) (0.878; 95% CI, 0.800-0.957) and thus showed the best diagnostic performance in discriminating between milk-allergic and non-milk allergic patients, with a sensitivity of 92.6% and specificity of 72.9% using a statistically optimal cut-off value (OD450nm, 0.191). The combinations of Bos d 5 + Bos d 12 showed an AUC of 0.926, which was larger than for any individual components. CONCLUSIONS: Our results revealed inter-individual variation in the sensitization to different milk allergen component. Bos d 12 sIgE showed best performance in diagnosing milk allergy. Milk allergy diagnostic accuracy was further improved using combinations of milk allergen components by application of ROC curves based on logistic regression.


Assuntos
Alérgenos/imunologia , Lipocalinas/imunologia , Hipersensibilidade a Leite/diagnóstico , Animais , Bovinos , Pré-Escolar , China , Diagnóstico Diferencial , Feminino , Humanos , Imunoglobulina E/sangue , Lactente , Recém-Nascido , Masculino , Valor Preditivo dos Testes , Prognóstico , Padrões de Referência , Sensibilidade e Especificidade
9.
Anal Bioanal Chem ; 410(5): 1501-1510, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29247381

RESUMO

The determination of specific IgE (sIgE) level is of great importance in IgE-mediated food allergies. Our aim was to develop a homogeneous immunoassay-light-initiated chemiluminescent assay (LICA)-for measuring allergen sIgE of a single component in egg white, thus evaluating the LICA-sIgE assay as a useful tool in the diagnosis of food allergy. The LICA-sIgE assay was performed by incubating serum sample with anti-human IgE antibody coated with chemiluminescer beads, streptavidin-coated sensitizer beads, and biotinylated antigens, which consist of four components in egg white. Serum samples from egg allergic patients (n = 70) and healthy volunteers (n = 30) were collected. For calibration, purified human IgE was used as the calibrator. Working conditions of this homogeneous immunoassay were optimized, analytical performance was determined, and correlation of the results between LICA and ImmunoCAP was evaluated. The assays were performed in 8-well plates with a sample volume diluted to 1:10 of 25 µl. Intra-assay precision (% coefficient of variation) ranged from 1.83 to 4.13%, and inter-assay precision ranged from 2.70 to 8.70%. It exhibited excellent sensitivity, which could distinguish between positive samples and negative samples even at a large dilution level. The sIgE-LICA and ImmunoCAP correlated well in patients allergic to single component (r 2 = 0.929). Also, the components ovomucoid and ovalbumin were best at predicting ImmunoCAP results, with the same area under the ROC curve (AUC) of 0.81, and a specificity of 90.0 and 93.3%, respectively. Our data show effective performance characteristics of LICA to detect sIgE in human serum based on component-resolved diagnostic tests (CRD). The homogeneous sIgE-LICA assay has the following key advantages: requires no washing, simplicity and rapidity, reproducibility, high-throughput, good performance in a liquid phase assay, and good suitability for sIgE diagnosis in food allergy based on CRD. Graphical abstract A light-initiated chemiluminescent assay was developed for the quantitation of sIgE against egg white allergens based on component-resolved diagnosis. Components Gal d 1 and Gal d 2 with the highest AUC values of 0.81 were considered the best at predicting egg allergy.


Assuntos
Alérgenos/química , Clara de Ovo/química , Imunoensaio/métodos , Luz , Medições Luminescentes/métodos , Alérgenos/sangue , Sítios de Ligação de Anticorpos , Análise Química do Sangue , Hipersensibilidade Alimentar/imunologia , Humanos , Limite de Detecção , Modelos Biológicos , Padrões de Referência , Reprodutibilidade dos Testes , Estreptavidina/química , Fatores de Tempo
10.
Bioresour Technol ; 243: 1087-1096, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28764115

RESUMO

A continuous microbial fuel cell system was constructed treating ammonium/organics rich wastewater. Operational performance of MFC system, mechanisms of ammonium removal, effect of ammonium on organics removal and energy output, C and N balance of anode chamber and microbial community analysis of anode chamber were studied. It was concluded that 0.0914kg/m3d NH4+-N and 5.739kg/m3d COD were removed from anode chamber and simultaneous nitrification and denitrification (SND) occurred in cathode chamber resulting in COD, TN removal rate of 88.53%, 71.35% respectively. Excess ammonium affected energy output and the MFC system reached maximum energy output of 816.8mV and 62.94mW/m3. In anode chamber, Spirochaetes bacterium sp., Methanobacterium formicicum sp. was predominant in bacteria, archaea communities respectively which contributed to wastewater treatment and electricity generation. This study showed the potential for practical application of continuous flow MFC system treating ammonium/organics rich wastewater and achieving electricity generation simultaneously.


Assuntos
Compostos de Amônio , Fontes de Energia Bioelétrica , Eletricidade , Desnitrificação , Águas Residuárias
11.
Environ Technol ; 38(24): 3074-3083, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28142646

RESUMO

Fracturing waste liquid (FWL) is generated during shale gas extraction and contains high concentrations of suspended solid, salinity and organic compounds, which needs proper management to prevent excessive environmental disruption. Biological treatment of the FWL was attempted in this study using a membrane-coupled internal circulation aerobic biological fluidized bed (MC-ICABFB) after being treated by coagulation. The results showed that poly aluminum chloride (PAC) of 30 g/L, polyacrylamide (PAM) of 20 mg/L and pH of 7.0 were suitable choices for coagulation. The pretreated FWL mixed with synthetic wastewater at different ratios were used as the influent wastewater for the reactor. The MC-ICABFB had relatively good performance on COD and NH4+-N removal and the main residual organic compound in the effluent was phthalates according to the analysis of GC-MC profiles. In addition, a suitable pretreatment process for the FWL to facilitate biological treatment of the wastewater needs further research.


Assuntos
Reatores Biológicos , Fraturamento Hidráulico , Eliminação de Resíduos Líquidos , Águas Residuárias/química , Resinas Acrílicas/análise , Aerobiose , Hidróxido de Alumínio/análise , Concentração de Íons de Hidrogênio
12.
Mol Nutr Food Res ; 60(10): 2275-2287, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27240706

RESUMO

SCOPE: Crab is a major source of shellfish allergens. Most studies have focused on allergens in crab muscle (CM) rather than on allergens in crab ovary (CO). This study aimed to identify potential allergens in CO from Eriocheir sinensis. METHODS AND RESULTS: Dot blot and immunoblotting results revealed the differential reactivity of CM and CO extracts to positive sera from patients allergic to crabs. Four CO proteins showed good specific IgE-binding activities in 2-DE/immunoblotting analysis; mass spectrometry identified the proteins as hemocyanin, vitellogenin, ovary development-related protein EJO1and EJO2. The recently identified allergen EJO1 is named 'Eri s 2' in the World Health Organization and International Union of Immunological Societies (WHO/IUIS) allergen nomenclature database. Recombinant Eri s 2 exhibited good reactivity to positive sera, and pre-incubation with recombinant Eri s 2 abrogated the reactivity of positive sera from two patients to CO in a dose-dependent manner. Moreover, co-incubation of recombinant Eri s 2 with patient basophils dose-dependently promoted basophil activation, confirming the biological activity of Eri s 2. CONCLUSION: CO tissue is an important allergen source, and Eri s 2 is a new crab allergen. This study provides insights that will be useful for component-resolved diagnostics for crab allergy.


Assuntos
Alérgenos/química , Alérgenos/imunologia , Braquiúros/imunologia , Ovário/química , Hipersensibilidade a Frutos do Mar/imunologia , Adolescente , Adulto , Idoso , Alérgenos/genética , Animais , Basófilos/imunologia , Western Blotting , Braquiúros/química , Criança , Pré-Escolar , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Immunoblotting , Lactente , Masculino , Pessoa de Meia-Idade , Ovário/imunologia
13.
Mol Immunol ; 74: 125-32, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27208437

RESUMO

Crab meat and roe are highly nutritious delicacies in China. While extensive research has been conducted for allergens derived from crab-meat, data relevant to the allergenic potential of crab roe derived proteins, of which hemocyanin is a principal contender, are almost entirely absent. Using bioinformatics prediction and IgE-binding assays, the three principal immunodominant epitopes of hemocyanin were identified and then combined as a single recombinant fusion protein (rHc). This together with the full-length recombinant protein (Hc) were expressed in Escherichia coli and subsequently identified by SDS-PAGE and immunoblotting. Ninety-five percent of our patients were found to carry rHc-specific IgE antibodies by ELISA. Dot-blot inhibition, together with ELISA inhibition studies, showed that pre-incubation of patient sera with the recombinant epitope protein could inhibit26% to 63% (mean: 50%) of IgE binding to immobilized, full-length Hc and the dose-response curve represents as a sigmoid shape. The recombinant protein (rHc) represents a versatile biologic tool with which to diagnose and investigate therapies for E. sinensis allergy.


Assuntos
Braquiúros/imunologia , Hipersensibilidade Alimentar/diagnóstico , Hipersensibilidade Alimentar/imunologia , Hemocianinas/imunologia , Epitopos Imunodominantes/imunologia , Adulto , Alérgenos/imunologia , Animais , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Immunoblotting , Imunoglobulina E/imunologia , Lactente , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Proteínas Recombinantes/imunologia , Adulto Jovem
14.
J Sleep Res ; 25(2): 169-80, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26420665

RESUMO

Substantial individual differences characterize the changes induced by total sleep deprivation on cognitive functions. Despite some progress having been achieved, the mechanisms of individual differences in response to total sleep deprivation have not been clearly elucidated. Cerebral metabolism in the resting state is among the key physiological processes supporting the daily function of the brain, and may play an important role in these individual differences. Twenty-two right-handed participants (nine females and 13 males) between 20 and 26 years old completed a mathematical processing task both in resting wakefulness and after 24 h of total sleep deprivation. Fluorine-18 fluorodeoxyglucose positron emission tomography-computed tomography was used to investigate brain metabolism changes. The mathematical task was performed after the positron emission tomography scans were completed. Correlation analysis was used to investigate the correlations between cognitive performance changes and brain metabolism changes. Large inter-individual differences were found in the throughput changes, but these inter-individual differences were not associated with baseline or post-deprivation performance levels. Specifically, deterioration of throughput on the mathematical processing task was significantly correlated with metabolism changes in the superior frontal medial gyrus. These findings suggested that frontal metabolic activity contributes to individual differences in waking-induced impairment of cognitive performance.


Assuntos
Cognição , Lobo Frontal/metabolismo , Individualidade , Privação do Sono/fisiopatologia , Privação do Sono/psicologia , Adulto , Feminino , Lobo Frontal/fisiopatologia , Humanos , Masculino , Tomografia por Emissão de Pósitrons , Descanso , Fatores de Tempo , Vigília , Adulto Jovem
15.
Environ Technol ; 36(24): 3210-20, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26111205

RESUMO

An aerobic moving bed biofilm reactor (MBBR) was adopted to treat Lurgi coal gasification waste water (LCGW) in about 10 months. The pollutant load and dissolve oxygen (DO) concentration were adjusted by trying to maximize the accumulation of [Formula: see text] in the MBBR for LCGW treatment. The highest [Formula: see text] accumulation proportion [Formula: see text] was 73.9%, but was not stable with influent chemical oxygen demand (COD) and DO concentrations of around 1000 and 1.5 mg/L, respectively. Around 1500 mg/L of influent COD concentration and 1.5 mg/L of DO concentration were proper operation conditions for the aerobic MBBR to achieve relatively stable [Formula: see text] accumulation, with [Formula: see text] ratio at 53% and [Formula: see text] ratio at just 4.3% in the effluent. More specifically, free ammonia concentration and DO concentration affected [Formula: see text] accumulation much more obvious than phenols concentration. The activity and quantity of nitrifying bacteria growth in suspended sludge and biofilm of the MBBR were monitored simultaneously to explain the variations of [Formula: see text] accumulation performance under different operation conditions. An aerobic moving bed biofilm reactor (MBBR) was adopted to treat Lurgi coal gasification waste water (LCGW)in about 10 months. The pollutant load and dissolve oxygen (DO) concentration were adjusted by trying to maximize the accumulation of NO(−)(2)−N in the MBBR for LCGW treatment. The highest NO(−)(2)−N accumulation proportion(NO(−)(2)−Neffluent/TN effluent) was 73.9%, but was not stable with influent chemical oxygen demand (COD) and DO concentrations of around 1000 and 1.5 mg/L, respectively. Around 1500 mg/L of influent COD concentration and 1.5 mg/L of DO concentration were proper operation conditions for the aerobic MBBR to achieve relatively stable NO(−)(2)−N accumulation,with NO(−)(2)−N/TN ratio at 53% and NO(-)(3)−N/TN ratio at just 4.3% in the effluent. More specifically, free ammonia concentration and DO concentration affected NO(2)(−)N accumulation much more obvious than phenols concentration. The activity and quantity of nitrifying bacteria growth in suspended sludge and biofilm of the MBBR were monitored simultaneously toexplain the variations of NO(−)(2)−N accumulation performance under different operation conditions.


Assuntos
Fenômenos Fisiológicos Bacterianos , Biofilmes , Reatores Biológicos/microbiologia , Nitritos/metabolismo , Oxigênio/análise , Eliminação de Resíduos Líquidos/instrumentação , Águas Residuárias/análise , Biodegradação Ambiental , Análise da Demanda Biológica de Oxigênio , Carvão Mineral , Gases/química
17.
Zhongguo Fei Ai Za Zhi ; 17(2): 148-54, 2014 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-24581167

RESUMO

BACKGROUND AND OBJECTIVE: Drug resistance is a major obstacle on lung cancer treatment and Vinorelbine is an effective drug to inhibition of tumor proliferation and metastasis. In this study, we investigated the effect and mechanism of Vinorelbine on reversing the cisplatin resistance of human lung cancer A549/DDP cell line. METHODS: With 1 µmol/L and 5 µmol/L Vinorelbine treatment, MTS assay was employed to determine the effect of the cisplatin sensitivity of tumor cells, flow cytometry to determine the apoptosis rate and change of Rh-123 content; Western blot to determine the expression of MDR1, Bcl-2, surviving, PTEN, caspase-3/8 and phosphorylation level of Akt (p-Akt); Real-time PCR was to determine the mRNA expression of MDR1, Bcl-2, survivin and PTEN. Finally the transcriptional activities of NF-κB, Twist and Snail were determined by reporter gene system. RESULTS: With 1 µmol/L and 5 µmol/L Vinorelbine treatment, the sensitivity of cancer cells to cisplatin was increased by 1.91- and 2.54- folds respectively, flow cytometry showed that the content of Rh-123 was elevated 1.93- and 2.95- folds and apoptosis rate was increased 2.25- and 3.82- folds, Western blot showed that the expression of multidrug resistance related proteins MDR, Bcl-2 and survivin were downregulated, caspase-3/8 and PTEN was upregulated, phosphorylation of Akt was downregulated as well, real-time assay showed that the mRNA expression of MDR1 was downregulated 43.5% and 25.8%, Bcl-2 was downregulated 57.3% and 34.1%, survivin was downregulated 37.6% and 12.4%, PTEN was upregulated 183.4% and 154.2%, the transcriptional activities of NF-κB was downregulated 53.2% and 34.5%, Twist was downregulated 61.4% and 33.5%, and Snail was downregulated 57.8% and 18.7%. CONCLUSION: Vinorelbine treatment led to increase of cisplatin sensitivity of A549/DDP cells and the mechanisms included the regulation of PTEN/AKT/NF-κB signal pathway to decreased drug resistance gene expression and increased pro-apoptosis gene expression.


Assuntos
Antineoplásicos/farmacologia , Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos , Neoplasias Pulmonares/fisiopatologia , Vimblastina/análogos & derivados , Apoptose/efeitos dos fármacos , Caspase 3/genética , Caspase 3/metabolismo , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , NF-kappa B/genética , NF-kappa B/metabolismo , PTEN Fosfo-Hidrolase/genética , PTEN Fosfo-Hidrolase/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Vimblastina/farmacologia , Vinorelbina
18.
Langmuir ; 29(12): 3943-9, 2013 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-23445300

RESUMO

Multilayer films composed of azide-functional polymer and polyphenylene dendrimer-stabilized gold nanoparticles with alkynes in their peripheries have been fabricated using a layer-by-layer (LBL) approach via "click" chemistry. This method permits facile covalent linking of the polymer/nanoparticle interlayers in the mixture of DMF and water, which provides a general and powerful technique for preparing uniform nanoparticle (NP) thin films. The deposition process is linearly related to the number of bilayers as monitored by UV-vis spectroscopy. The multilayer structure and morphology have been characterized by X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and contact angle.


Assuntos
Dendrímeros/química , Ouro/química , Nanopartículas Metálicas/química , Polímeros/química , Alquinos/química , Azidas/química , Química Click , Nanopartículas Metálicas/ultraestrutura , Microscopia de Força Atômica , Microscopia Eletrônica de Transmissão , Espectroscopia Fotoeletrônica , Propriedades de Superfície
19.
J Hazard Mater ; 241-242: 241-51, 2012 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-23040315

RESUMO

As past studies presented, there is obvious defect that the fillers in the Fe(0)/GAC reactor begin to be passive after about 60 d continuous running, although the complicated, toxic and refractory ABS resin wastewater can be pretreated efficiently by the Fe(0)/GAC reactor. During the process, the Fe(3)(PO(4))(2) and FePO(4) crystals with high density in the passive film are formed by the reaction between PO(4)(3-) and Fe(2+)/Fe(3+). Meanwhile, they obstruct the formation of macroscopic galvanic cells between Fe(0) and GAC, which will lower the wastewater treatment efficiency of Fe(0)/GAC reactor. In this study, in order to remove the Fe(3)(PO(4))(2) and FePO(4) crystals on the surface of the passive fillers, the bacteria were acclimated in the passive Fe(0)/GAC reactor. According to the results, it can be concluded that the Fe(3)(PO(4))(2) and FePO(4) crystals with high density in the passive film could be decomposed or removed by the joint action between the typical propionic acid type fermentation bacteria and sulfate reducing bacteria (SRB), whereas the PO(4)(3-) ions from the decomposition of the Fe(3)(PO(4))(2) and FePO(4) crystals were released into aqueous solution which would be discharged from the passive Fe(0)/GAC reactor. Furthermore, the remained FeS and sulfur (S) in the passive film also can be decomposed or removed easily by the oxidation of the sulfur-oxidizing bacteria. This study provides some theoretical references for the further study of a cost-effective bio-regeneration technology to solve the passive problems of the fillers in the zero-valent iron (ZVI) or Fe(0)/GAC reactor.


Assuntos
Biofilmes/crescimento & desenvolvimento , Carvão Vegetal/química , Compostos Férricos/isolamento & purificação , Compostos Ferrosos/isolamento & purificação , Ferro/química , Fosfatos/isolamento & purificação , Purificação da Água/métodos , Resinas Acrílicas/química , Adaptação Fisiológica , Bactérias Aeróbias/crescimento & desenvolvimento , Bactérias Aeróbias/fisiologia , Butadienos/química , Cristalização , Arquitetura de Instituições de Saúde , Microscopia Eletrônica de Varredura , Poliestirenos/química , Espectrometria por Raios X , Propriedades de Superfície , Águas Residuárias/química , Purificação da Água/instrumentação
20.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 28(8): 871-3, 2012 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-22863598

RESUMO

AIM: To establish a sensitive biotin-avidin enzyme linked immunosorbent assay (BA-ELISA) method for detecting carcinoembryonic antigen (CEA) in serum. METHODS: CEA which had been purified by affinity chromatography was used to immunize the rabbits to produce polyclonal antibodies. Then the antibodies were connected with biotin and horseradish peroxidase (HRP). So BA-ELISA method was established on the basis of 96 microwell plates coated with biotinylated BSA. Finally we examined the sensitivity, specificity, stability and recovery rate of this system and compared the BA-ELISA method with the traditional ELISA, radioimmunoassay and chemiluminescence in detecting CEA concentrations. RESULTS: The stability of this system was proved good. The linear range was from 0.42 to 50 U/mL, the sensitivity was 0.42 U/mL, and the intra-differences together with inter-differences were less than 10.0%. There was significant difference between BA-ELISA and traditional ELISA, while there was no significant difference between BA-ELISA and radioimmunoassay. The regression equation of this method was y=0.04825+0.99674x and r=0.994, and there was no significant difference between the BA-ELISA and chemiluminescence. CONCLUSION: The BA-ELISA method we established to detect CEA was easy to operate, highly sensitive, low in price and suitable for application in clinical detection.


Assuntos
Antígeno Carcinoembrionário/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Animais , Avidina , Biotina , Neoplasias da Mama/sangue , Antígeno Carcinoembrionário/imunologia , Antígeno Carcinoembrionário/isolamento & purificação , Feminino , Humanos , Coelhos , Valores de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
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