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1.
Sci Total Environ ; 830: 154783, 2022 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-35339549

RESUMO

In this study, microbes were added to food waste compost in order to investigate the bioaugmentation mechanism of Humic acid (HA) formation. Thermogravimetric analysis, structural equation model, Fourier transform infrared spectroscopy and statistical analysis were utilized to explain the bioaugmentation mechanism. The results showed that bioaugmentation increased humification rate and degree. Bioaugmentation not only promoted the formation of aromatic structures and CC bonds but also brought different change orders of functional groups in HA. The HA obtained in bioaugmentation group (BA, 7.51 g/kg) was significantly higher compared to the control group (CK, 2.37 g/kg). Similarly, the HA/FA of BA (1.90) was also higher than that of CK (0.62), and peaked at 2.34 on day 40. The polyphenol humification pathway played a major role regardless of the addition of inoculant. However, the exogenous microbes promoted protein and carbohydrate degradation in the initial stage, and the abundance of precursors (amino acids and reducing sugars) enhanced both Maillard and polyphenol humification pathways. When polyphenol was insufficient in later stage, bioaugmentation mainly embodied in the strengthening of Maillard humification pathway. This finding benefited the practice of directional humification process of food waste composting.

2.
J Environ Sci (China) ; 113: 1-11, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34963519

RESUMO

Although disinfection byproducts (DBPs) in drinking water have been suggested as a cancer causing factor, the causative compounds have not yet been clarified. In this study, we used liquid chromatography quadrupole-time-of-flight spectrometry (LC-QTOF MS) to identify the unknown disinfection byproducts (DBPs) in drinking water produced from Taihu Lake source water, which is known as a convergence point for the anthropogenic pollutants discharged from intensive industrial activities in the surrounding regions. In total, 91 formulas of DBPs were discovered through LC-QTOF MS nontarget screen, 81 of which have not yet been reported. Among the 91 molecules, 56 only contain bromine, 15 only contain chlorine and 20 DBPs have both bromine and chlorine atoms. Finally, five DBPs including 2,4,6-tribromophenol, 2,6-dibromo-4-chlorophenol, 2,6-dichloro-4-bromophenol, 4-bromo-2,6-di-tert-butylphenol and 3,6-dibromocarbazole were confirmed using standards. The former three compounds mainly formed in the predisinfection step (maximum concentration, 0.2-2.6 µg/L), while the latter two formed in the disinfection step (maximum concentration, 18.2-33.6 ng/L). In addition, 19 possible precursors of the discovered DBPs were detected, with the aromatic compounds being a major group. 2,6-di-tert-butylphenol as the precursor of 4-bromo-2,6-di-tert-butylphenol was confirmed with standard, with a concentration of 20.3 µg/L in raw water. The results of this study show that brominated DBPs which are possibly formed from industrial pollutants are relevant DBP species in drinking water produced form Taihu source water, suggesting protection of Taihu Lake source water is important to control the DBP risks.


Assuntos
Desinfetantes , Água Potável , Poluentes Químicos da Água , Purificação da Água , Bromo , Desinfetantes/análise , Desinfecção , Água Potável/análise , Halogenação , Lagos , Espectrometria de Massas por Ionização por Electrospray , Poluentes Químicos da Água/análise
3.
J Asian Nat Prod Res ; : 1-10, 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34762536

RESUMO

Two new prenylated xanthones, mangoxanthones A-B (1-2), together with four known compounds 3-6, were isolated from the ethanol extract of the pericarp of Garcinia mangostana. The structures of these compounds have been elucidated based on spectroscopic analysis. The analysis results of chiral HPLC revealed compounds 1 and 2 were scalemic mixtures respectively. All isolated compounds were biologically evaluated for their α-glucosidase and α-amylase inhibitory effects using in-vitro assays. Compound 1 showed moderate inhibitory activities against α-glucosidase and α-amylase with IC50 of 29.06 ± 1.86 and 22.74 ± 2.07 µM, respectively. Molecular docking predicted the binding sites of compound 1 to α-glucosidase and α-amylase. A preliminary structure-activity relationship was discussed.

5.
mSystems ; 6(6): e0066721, 2021 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-34726497

RESUMO

nirK-type and nirS-type denitrifier communities mediate the conversion of nitrite to nitric oxide, which is the key step in denitrification. Results of previous studies have indicated that nirK-type and nirS-type denitrifiers may occupy different niches; however, the mechanisms and drivers of their responses to environmental changes within community assembly are poorly understood. In this study, we evaluated the distribution and assembly of nirK-type and nirS-type denitrifier communities along an elevation gradient from 1,800 to 4,100 m at Mount Gongga, China. Results showed that elevational patterns of alpha diversity in nirK-type and nirS-type denitrifier communities followed hump-backed patterns along the elevation gradient. However, nirK-type denitrifier communities formed two distinct clusters that were primarily separated by elevation, whereas nirS-type denitrifier communities formed three distinct clusters that were primarily separated by forest type along the elevation gradient. Moreover, deterministic processes were dominant in governing the assemblages of nirK-type and nirS-type denitrifiers. Soil pH was a key factor influencing the alpha and beta diversity of the nirK-type denitrifier communities, whereas plant richness was a primary variable influencing nirS-type denitrifiers. Additionally, our work revealed that soil denitrification potential was mainly explained by the variation in the beta diversity of denitrifier communities rather than the alpha diversity of denitrifier communities or denitrifier abundances over a large elevation gradient, and nirK-type denitrifiers played more important roles in soil denitrification. These results may contribute to predicting the consequences of global changes on denitrifier communities and their ecological services. IMPORTANCE Mount Gongga is the highest peak in the Hengduan Mountain region and is located at the southeastern fringe of the Tibetan Plateau, Sichuan Province, southwest China. As a transitional zone between the Tibetan Plateau and Sichuan Basin, Gongga Mountain features particularly diverse topography, geology, climate, and biodiversity and is a globally significant hot spot of biodiversity. In this contribution, we comprehensively describe the diversity and assembly of denitrifier communities along an elevation gradient on Gongga Mountain. Our findings established for the first time that the distribution patterns of beta diversity and driving factors differed between nirK-type and nirS-type denitrifier communities, and deterministic processes were dominant in shaping communities of denitrifiers. Moreover, the beta diversity of denitrifier communities rather than alpha diversity or denitrifier abundance played an important role in explaining denitrification potential, and the beta diversity of nirK-type denitrifier communities was more important than nirS-type denitrifier communities in soil denitrification. This work provides crucial insights into the spatial distribution of denitrifier communities and their ecological function and increases our understanding of the mechanisms underlying spatial distribution of community assembly along large elevation gradients.

6.
Chem Asian J ; 16(22): 3774-3780, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34605208

RESUMO

Sodium-ion batteries are promising energy-storage systems, but they are facing huge challenges for developing fast-charging anode materials. Bismuth (Bi)-based anode materials are considered as candidates for fast-charging anodes of sodium-ion batteries due to their excellent rate performance. Herein, we designed a two-dimensional Bi/MXene anode material based on a hydrogen thermal reduction strategy. Benefitting from microstructure advantages, Bi/MXene anodes exhibited an excellent rate capability and superior cycle performance in Na//Bi/MXene half-batteries and Na3 V2 (PO4 )3 /C//Bi/MXene full-batteries. Moreover, full-batteries can complete a charge/discharge cycle in 7 min and maintain an excellent cycle life (over 7000 cycles). The electrochemical test results showed that Bi/MXene is a promising anode material with fast charge/discharge capability for sodium-ion batteries.

7.
ChemSusChem ; 14(23): 5293-5303, 2021 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-34582117

RESUMO

Although it is a promising sodium storage material due to its excellent electrochemical activity, small bandgap, and large interlayer spacing, layered molybdenum disulfide (MoS2 ) suffers from poor rate capability and degraded cycling life, resulting from its serious aggregation upon preparation, sluggish reaction kinetics, and structure expansion during cycling. To address these issues, a polyethyleneimine (PEI)-assisted fabrication approach was developed for the rational synthesis of an interconnected framework with nitrogen-doped carbon-confined MoS2 nanosheets/Ti3 C2 Tx MXene (MoS2 /Ti3 C2 Tx @NC), where the PEI could guide the uniform growth of MoS2 on Ti3 C2 Tx and the self-generated NC simultaneously enhanced its synergistic coupling with MoS2 /Ti3 C2 Tx , thus contributing to the improvement of charge transfer, diffusion kinetics, and structural integrity of the hybrid electrode. Consequently, the desired MoS2 /Ti3 C2 Tx @NC delivered impressive sodium storage performance, demonstrating high reversible capacities of 397.3 and 206.8 mAh g-1 at 0.1 A g-1 after 100 cycles and 0.5 A g-1 after 500 cycles, respectively. Moreover, electrochemical kinetics analysis and charge storage mechanism manifested that high capacitive contribution, facilitated Na+ transport pathways, and synergistic electronic coupling between MoS2 /Ti3 C2 Tx and NC contributed to the superior sodium storage performance.

8.
J Colloid Interface Sci ; 604: 368-377, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34265691

RESUMO

Transition metal sulfides, as an important class of inorganics, have been shown to be potential high-performance electrode candidates for lithium-ion batteries (LIBs) in account of their high activity towards lithium storage, rich types and diverse structures. Despite these advantages, structure degradation related with volume variations upon electrochemical cycling restricts their further development. In this present study, a unique hybrid structure with ultrafine heazlewoodite nanoparticles (less than 10 nm) in-situ confined in nitrogen and sulfur dual-doped carbon (Ni3S2@NSC) was constructed though a facile pyrolysis process, using a novel Ni-based metal chelates as the precursor. Specifically, enhanced structure stability, shortened Li+ migration distance and improved reaction dynamics can be obtained simultaneously in the designed structure, thereby allowing to realize high lithium storage performance. Consequently, a remarkable reversible capacity of 955.9 mAh g-1 (0.1 A g-1 after 100 cycles) and a superior long-term cycling stability up to 1200 cycles (863.7 mAh g-1 at 1.0 A g-1) are obtained. Importantly, the fundamental understanding on the improved lithium storage of Ni3S2@NSC based on the synergistic coupling reveals that the combination between Ni3S2 and NSC at the hetero-interface through the doped sulfur atoms contributes to the integrity of electrode and improved kinetics.

9.
Chemistry ; 27(50): 12900-12909, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34181786

RESUMO

Spinel ferrites are promising anode materials for lithium-ion batteries (LIBs) owing to their high theoretical specific capacities. However, their practical application is impeded by inherent low conductivity and severe volume expansion, which can be surpassed by increasing the surface-to-volume ratio of nanoparticles. Currently, most methods produce spinel ferrite nanoparticles with large size and severe aggregation, degrading their electrochemical performance. In this study, a low-temperature aminolytic route was designed to synthesize sub-10 nm CoFe2 O4 nanoparticles with good dispersion through carefully exploiting the reaction of acetates and oleylamine. The performance of CoFe2 O4 nanoparticles obtained by a traditional co-precipitation method was also investigated for comparison. This work demonstrates that CoFe2 O4 nanoparticles synthesized by the aminolytic route are promising as anode materials for LIBs. Besides, this method can be extended to design other spinel ferrites for energy storage devices with superior performance by simply changing the starting material, such as MnFe2 O4 , MgFe2 O4 , ZnFe2 O4 , and so on.

10.
Chem Asian J ; 16(16): 2314-2320, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34159728

RESUMO

As two-dimensional (2D) materials, bismuth (Bi) has large interlayer spacing along c-axis (0.395 nm) which provides rich active sites for sodium ions, thus guaranteeing high sodium ion storage activity. However, its poor electrical conductivity, combined with its degraded cycling performance, restricts its practical application. Herein, Bi microsphere coated with nitrogen-doped carbon (Bi@NC) was synthesized. Owing to the unique Bi crystals and nitrogen-doped carbon layer, the obtained Bi@NC anode exhibited satisfactory cycling stability and superior rate capability. Moreover, after assembling Bi@NC anode with Na3 V2 (PO4 )3 @C cathode to full battery, excellent sodium storage performance was obtained (57 mA h g-1 after 2000 cycles at 1.0 A g-1 ).

11.
Comp Cytogenet ; 15(2): 137-148, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34055237

RESUMO

Eight taxa of Sorbus Linnaeus, 1753 sensu stricto (Rosaceae) from China have been studied karyologically through chromosome counting, chromosomal measurement and karyotype symmetry. Genome size was also estimated by flow cytometry. Six taxa, S. amabilis Cheng ex T.T.Yu et K.C.Kuan, 1963, S. hupehensis var. paucijuga (D.K. Zang et P.C. Huang, 1992) L.T. Lu, 2000, S. koehneana C.K. Schneider, 1906, S. pohuashanensis (Hance, 1875) Hedlund, 1901, S. scalaris Koehne, 1913 and S. wilsoniana C.K. Schneider, 1906 are diploids with 2n = 34, whereas two taxa, S. filipes Handel-Mazzetti,1933 and S. ovalis McAllister, 2005 are tetraploid with 2n = 68. In general, the chromosome size is mainly small, and karyotypes are symmetrical with predominance of metacentric chromosomes. Genome size variation of diploids and tetraploids is 1.401 pg -1.676 pg and 2.674 pg -2.684 pg, respectively. Chromosome numbers of S. amabilis and S. hupehensis var. paucijuga, and karyotype and genome size of eight taxa studied are reported for the first time. This study emphasised the reliability of flow cytometry in genome size determination to infer ploidy levels in Chinese native Sorbus species.

12.
J Colloid Interface Sci ; 594: 35-46, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33756366

RESUMO

Nickel sulfide based anode materials, featuring rich types, high specific capacities and favorable conversion kinetics, have been proved to be promisingly applied in high-performance sodium-ion batteries (SIBs). Unfortunately, the poor electronic/ionic conductivity, together with the structure change induced degraded capacity upon cycling, restricts their further development. In this work, heazlewoodite nanoparticles decorated on nitrogen doped reduced graphene oxide (Ni3S2/NrGO) were fabricated via a facile combined approach with freeze-drying and subsequent in-situ sulfidation. In the obtained hybrid structure, the synergistic effect between Ni3S2 and NrGO endows the composite with highly conductive pathways, thus accelerating the charge transfer. Benefitting from the buffering matrix offered by NrGO as well as the tight combination between Ni3S2 and NrGO, this novel Ni3S2/NrGO demonstrates satisfying sodium storage performance, with a stable reversible capacity of 299.2 mAh g-1 up to 100 cycles (0.1 A g-1) and a high initial Coulombic efficiency of 76.8%. Importantly, the rational structure design and synthesis method, as well as the insights on the improved electrochemical performance reported in this work, should be helpful for the development of new-type host materials with high performance for SIBs.

13.
Appl Environ Microbiol ; 87(11)2021 05 11.
Artigo em Inglês | MEDLINE | ID: mdl-33741616

RESUMO

Lactate-driven chain elongation (LCE) has emerged as a new biotechnology to upgrade organic waste streams into a valuable biochemical and fuel precursor, medium-chain carboxylate, n-caproate. Considering that a low cost of downstream extraction is critical for biorefinery technology, a high concentration of n-caproate production is very important to improve the scale-up of the LCE process. We report here that in a nonsterile open environment, the n-caproate concentration was increased from the previous record of 25.7 g·liter-1 to a new high level of 33.7 g·liter-1 (76.8 g chemical oxygen demand [COD]·liter - 1), with the highest production rate being 11.5 g·liter-1·day-1 (26.2 g COD·liter - 1·day-1). In addition, the LCE process remained stable, with an average concentration of n-caproate production of 20.2 ± 5.62 g·liter-1 (46.1 ± 12.8 g COD·liter - 1) for 780 days. Dynamic changes in taxonomic composition integrated with metagenomic data reveal the microbial ecology for long-term production of high concentrations of n-caproate: (i) the core microbiome is related to efficient functional groups, such as Ruminococcaceae (with functional strain CPB6); (ii) the core bacteria can maintain stability for long-term operation; (iii) the microbial network has relatively low microbe-microbe interaction strength; and (iv) low relative abundance and variety of competitors. The network structure could be shaped by hydraulic retention time (HRT) over time, and long-term operation at an HRT of 8 days displayed higher efficacy.IMPORTANCE Our research revealed the microbial network of the LCE reactor microbiome for n-caproate production at high concentrations, which will provide a foundation for designing or engineering the LCE reactor microbiome to recover n-caproate from organic waste streams in the future. In addition, the hypothetical model of the reactor microbiome that we proposed may offer guidance for researchers to find the underlying microbial mechanism when they encounter low-efficiency n-caproate production from the LCE process. We anticipate that our research will rapidly advance LCE biotechnology with the goal of promoting the sustainable development of human society.


Assuntos
Fenômenos Fisiológicos Bacterianos , Reatores Biológicos/microbiologia , Caproatos/metabolismo , Clostridiales/fisiologia , Ácido Láctico/química , Microbiota , Biodegradação Ambiental , Fermentação
14.
Clin Immunol ; 223: 108660, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33352295

RESUMO

PURPOSE: The study aimed to understand the molecular mechanisms that might lead to differences in the glucocorticoid response during sepsis. METHODS: Patients diagnosed with sepsis (n = 198) and 40 healthy controls were enrolled. Glucocorticoid receptor (GR) expression in circulating leukocytes and plasma levels of adrenocorticotropic hormone and cortisol on days 1 and 7 were measured in all participants. Expression profiling of 16 genes associated with GR expression in peripheral blood mononuclear cells (PBMCs) in 12 healthy controls and 26 patients with sepsis was performed by PCR. RESULTS: Cortisol levels were higher in patients with sepsis than in healthy controls on day 1 after admission and recovered to normal levels by day 7. GR expression was gradually downregulated in leukocyte subsets. Non-survivors showed lower GR and higher cortisol levels than survivors. GRα expression was lower in patients with sepsis than in controls, whereas GRß showed the opposite trend. MicroRNAs related to GR resistance and suppression were altered in PBMCs during sepsis. CONCLUSION: Patients with sepsis showed upregulated plasma cortisol levels along with downregulated GR expression on various leukocyte subtypes, portending poor cortisol response and outcome. Changes in GR-regulatory miRNAs may be responsible for GR low expression.


Assuntos
Glucocorticoides/uso terapêutico , Leucócitos Mononucleares/fisiologia , Receptores de Glucocorticoides/metabolismo , Sepse/tratamento farmacológico , Hormônio Adrenocorticotrópico/sangue , Idoso , Idoso de 80 Anos ou mais , Células Cultivadas , Estudos de Coortes , Feminino , Regulação da Expressão Gênica , Humanos , Hidrocortisona/sangue , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Receptores de Glucocorticoides/genética , Transcriptoma , Resultado do Tratamento
15.
ACS Appl Mater Interfaces ; 12(42): 47408-47415, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-32986395

RESUMO

Quaternary Cs2AgBiBr6 perovskites have been considered as a potential candidate to simultaneously resolve the lead toxicity and instability issues of unprecedented organic-inorganic hybrid halide perovskites. Unfortunately, the photovoltaic efficiency is still lower owing to the great challenge to make high-quality Cs2AgBiBr6 film with fewer defects. Herein, we demonstrate alkali metal ions including Li+, Na+, K+, and Rb+ as mediators to regulate the crystal lattice and film quality of Cs2AgBiBr6 perovskites. A less-pinhole perovskite film is obtained by precisely controlling the doping dosage and element species, significantly reducing the defects. When assembled into a hole-transporting material-free, carbon-electrode perovskite solar cell, a significantly enhanced efficiency of 2.57% compared to the undoped device with 1.77% efficiency has been achieved owing to the suppressed shunt current loss. Additionally, this device displays superior tolerance under high-temperature and air conditions without encapsulation, providing new opportunities to promote the future development of lead-free Cs2AgBiBr6 perovskites in the photoelectric field.

16.
Appl Microbiol Biotechnol ; 104(17): 7589-7602, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32686007

RESUMO

Seasonal dynamics of soil microbial communities may influence ecosystem functions and services. However, few observations have been conducted on the dynamics of a bacterial community assembly across seasons in different elevations in mountain forest ecosystems. In this study, the diversity, compositions, community assembly processes, and co-occurrence interactions of soil bacterial communities were investigated using Illumina sequencing of 16S rRNA genes across different seasons during two consecutive years (2016 and 2017) at two elevational sites in Mount Gongga, China. These two sites included an evergreen broad-leaved forest (EBF, 2100 m a.s.l.) and a dark coniferous forest (DCF, 3000 m a.s.l.). The results showed that bacterial diversity and structure varied considerably between the two elevational sites with only limited seasonal variations. Interannuality had a significant effect on the diversity and structure of soil bacterial communities. The bacterial alpha diversity was significantly higher at site EBF(e.g., OTUs richness, 2207 ± 276) than at site DCF(e.g., OTUs richness, 1826 ± 315). Soil pH, temperature, elevation, and water content were identified as important factors shaping soil bacterial communities in the mountain forests. Bacterial community assembly was primarily governed by deterministic processes regardless of elevation and season. Deterministic processes were stronger at site DCF than at EBF. The soil bacterial community at site EBF harbored a more complex and connected network with less resistance to environmental changes. Overall, this study showed that seasonal dynamics of bacterial communities were much weaker than those along elevations, implying that a single-season survey on a bacterial community along an elevational gradient can represent overall changes in the bacterial community. KEY POINTS: • Seasonal dynamics of soil bacterial communities were studied in Mount Gongga. • The bacterial community was mainly affected by elevation rather than season. • Deterministic processes dominated bacterial community assembly. • The bacterial network was more complex but less stable at EBF than at DCF.


Assuntos
Ecossistema , Solo , China , Florestas , RNA Ribossômico 16S/genética , Estações do Ano , Microbiologia do Solo
17.
Small ; 16(24): e2001812, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32431080

RESUMO

Albeit the effectiveness of surface oxygen vacancy in improving oxygen redox reactions in Li-O2 battery, the underpinning reason behind this improvement remains ambiguous. Herein, the concentration of oxygen vacancy in spinel NiCo2 O4 is first regulated via magnetron sputtering and its relationship with catalytic activity is comprehensively studied in Li-O2 battery based on experiment and density functional theory (DFT) calculation. The positive effect posed by oxygen vacancy originates from the up shifted antibond orbital relative to Fermi level (Ef ), which provides extra electronic state around Ef , eventually enhancing oxygen adsorption and charge transfer during oxygen redox reactions. However, with excessive oxygen vacancy, the negative effect emerges because the metal ions are mostly reduced to low valence based on the electrical neutral principle, which not only destabilizes the crystal structure but also weakens the ability to capture electrons from the antibond orbit of Li2 O2 , leading to poor catalytic activity for oxygen evolution reaction (OER).

18.
Innate Immun ; 26(6): 514-527, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32456597

RESUMO

This study aimed to investigate the possible relationship between the two biomarkers presepsin and procalcitonin (PCT) and monocyte immune function, and to explore their combination in mortality prediction in the early stage of sepsis. A total of 198 patients with bacterial infection and diagnosed with sepsis and 40 healthy control subjects were included. Blood samples were collected on admission within 24 h. Plasma concentrations of presepsin and PCT were measured. Expression of monocyte surface CD14, programmed cell death receptor ligand-1 (PD-L1) and human leucocyte Ag (HLA)-DR were determined using flow cytometry. Levels of plasma presepsin and PCT were significantly higher under septic conditions, and increased with the progression of sepsis. Monocyte CD14 and HLA-DR expression were decreased, while PD-L1 was overexpressed in sepsis compared to control. Presepsin and PCT concentrations were positively correlated with Sequential Organ Failure Assessment score, Acute Physiology and Chronic Health Evaluation System II score and PD-L1, while they were negatively correlated with CD14 and HLA-DR. Presepsin plus monocyte HLA-DR mean fluorescence intensity had the highest prognostic value over other parameters alone or in combination. Presepsin and PCT had a weak correlation with monocyte dysfunction during early sepsis. The combination of presepsin and monocyte HLA-DR could help improve prognostic value.


Assuntos
Biomarcadores/metabolismo , Antígenos HLA-DR/metabolismo , Receptores de Lipopolissacarídeos/metabolismo , Monócitos/fisiologia , Fragmentos de Peptídeos/metabolismo , Plasma/metabolismo , Pró-Calcitonina/metabolismo , Sepse/diagnóstico , Idoso , Idoso de 80 Anos ou mais , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Regulação para Cima
19.
Environ Mol Mutagen ; 61(5): 526-533, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32227502

RESUMO

Recently, halobenzoquinones (HBQs) disinfection byproducts, including 2,6-dichloro-1, 4-benzoquinone (DCBQ), 2,6-dichloro-3-methyl-1, 4-benzoquinone (DCMBQ), 2,3,6-trichloro-1, 4-benzoquinone (TCBQ), and 2,6-dibromobenzoquinone (DBBQ), have been of increasing concern due to their reported ability to induce oxidative damage, and thus genotoxicity. However, data on the risk of genotoxicity due to chromosomal damage by HBQs are still scarce. Here, the cytotoxicity and genotoxicity of the four HBQs were assessed using human cell lines (bladder cancer 5637 cells, colon carcinoma Caco-2 cells, and gastric MGC-803 cells). The four HBQs exhibited significant concentration-response relationships in all the three cell lines. Cytotoxicity of DCBQ, DCMBQ, TCBQ, and DBBQ, represented by the 50% concentration of inhibition (IC50 ) values, were 80.8-99.5, 41.0-57.6, 122.1-146.6, and 86.9-93.8 µM, respectively. The lowest effective concentrations for cellular micronuclei induction in the cell lines by DCBQ, DCMBQ, TCBQ, and DBBQ were 50-75, 20-41.5, 87.4-100, and 50 µM, respectively. 5637 and Caco-2 cells were more sensitive to the cytotoxic and genotoxic effects of HBQs than MGC-803 cells. These results show that HBQs can induce chromosomal damage; DCMBQ induced the highest cytotoxicity and genotoxicity in all the cell lines, and TCBQ caused the least toxicity.


Assuntos
Benzoquinonas/toxicidade , Desinfecção , Testes de Mutagenicidade , Mutagênicos/toxicidade , Morte Celular/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Humanos , Testes para Micronúcleos
20.
FEMS Microbiol Ecol ; 96(4)2020 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-32109277

RESUMO

Understanding microbial network assembly is a promising way to predict potential impacts of environmental changes on ecosystem functions. Yet, soil microbial network assembly in mountain ecosystems and its underlying mechanisms remain elusive. Here, we characterized soil microbial co-occurrence networks across 12 altitudinal sites in Mountain Gongga. Despite differences in habitats, soil bacterial networks separated into two different clusters by altitude, namely the lower and higher altitudes, while fungi did not show such a pattern. Bacterial networks encompassed more complex and closer relationships at the lower altitudes, while fungi had closer relationships at the higher altitudes, which could be attributed to niche differentiation caused by high variations in soil environments and plant communities. Both abiotic and biotic factors (e.g. soil pH and bacterial community composition) shaped bacterial networks. However, biotic factors played more important roles than the measured abiotic factors for fungal network assembly. Further analyses suggest that multiple mechanisms including niche overlap/differentiation, cross-feeding and competition between microorganisms could play important roles in shaping soil microbial networks. This study reveals microbial co-occurrence networks in response to different ecological factors, which provides important insights into our comprehensive understanding of microbial network assembly and their functional potentials in mountain ecosystems.


Assuntos
Ecossistema , Solo , Bactérias/genética , Fungos , Microbiologia do Solo
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