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1.
Neural Regen Res ; 18(1): 176-182, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35799539

RESUMO

Based on the Wallerian degeneration in the spinal cord pathways, the changes in synaptic connections, and the spinal cord-related cellular responses that alter the cellular structure of the brain, we presumed that brain diffusion tensor imaging (DTI) parameters may change after spinal cord injury. However, the dynamic changes in DTI parameters remain unclear. We established a Beagle dog model of T10 spinal cord contusion and performed DTI of the injured spinal cord. We found dynamic changes in DTI parameters in the cerebral peduncle, posterior limb of the internal capsule, pre- and postcentral gyri of the brain within 12 weeks after spinal cord injury. We then performed immunohistochemistry to detect the expression of neurofilament heavy polypeptide (axonal marker), glial fibrillary acidic protein (glial cell marker), and NeuN (neuronal marker). We found that these pathological changes were consistent with DTI parameter changes. These findings suggest that DTI can display brain structure changes after spinal cord injury.

2.
Neural Regen Res ; 18(3): 582-586, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36018181

RESUMO

Spinal cord injury is a severe and devastating disease, and spasticity is a common and severe complication that is notoriously refractory to treatment. However, the pathophysiological mechanisms underlying spasticity and its development remain largely unknown. The goal of the present study was to find differences, if any, in metabolites of the left precentral gyrus and basal ganglia of patients who have spinal cord injury with or without spasticity, and to explore the relationship between the brain metabolite concentrations and clinical status. Thirty-six participants were recruited for magnetic resonance spectroscopic examination: 23 with spinal cord injury (12 with spasticity and 11 without spasticity) and 13 healthy controls. We acquired localized proton spectra from the precentral gyrus and basal ganglia via 10 mm3 voxels. Notably, univariate linear regression analysis demonstrated that the lower that the N-acetylaspartate concentration (a marker for neuronal loss) was in the precentral gyrus of the patients, the lower their ASIA (American Spinal Injury Association) light-touch scores, pinprick scores, and motor scores. Additionally, longer durations of injury were associated with higher N-acetylaspartate levels in the precentral gyrus. Compared with the healthy participants and patients without spasticity, N-acetylaspartate levels in the patients with spasticity were significantly lower in both the precentral gyrus and basal ganglia. Lower N-acetylaspartate levels also correlated with greater sensory and motor dysfunction in the patients who had spinal cord injury with spasticity.

3.
J Clin Transl Hepatol ; 11(1): 110-117, 2023 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-36406330

RESUMO

Background and Aims: Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer-related death and ranks sixth in terms of incident cases worldwide. The purpose of this study was to develop an effective and sensitive method to distinguish liver cancer tissues from normal tissues in HCC patients. Integrin α6 is a promising cell surface target for molecular imaging of HCC, where it is overexpressed and is a prognostic biomarker. We previously identified an integrin α6-targeted peptide CRWYDENAC (RWY) that has been used for positron emission tomography (PET) imaging of HCC in mouse models. Methods: We labeled the integrin α6-targeted RWY peptide with cyanine 7 (Cy7) to form an optical probe (Cy7-RWY) for near infrared fluorescent (NIRF) and photoacoustic (PA) imaging in HCC. Mice transplanted with subcutaneous HCC-LM3 or orthotopic HCC-H22 cells that overexpressed integrin α6 were intravenously injected with Cy7-RWY and its corresponding Cy7-control. NIRF and PA images of mice were collected from 0 to 48 h after injection. Results: Both NIRF and PA signals started to accumulate in the tumor 2 h after injection of Cy7-RWY and peaked at 24 h. Conclusions: Cy7-RWY is a promising optical probe for NIRF and PA imaging of HCC in mice, and has potential clinical application for HCC detection.

4.
Neural Regen Res ; 18(4): 750-755, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36204831

RESUMO

The inability of damaged neurons to regenerate within the mature central nervous system (CNS) is a significant neuroscientific challenge. Astrocytes are an essential component of the CNS and participate in many physiological processes including blood-brain barrier formation, axon growth regulation, neuronal support, and higher cognitive functions such as memory. Recent reprogramming studies have confirmed that astrocytes in the mature CNS can be transformed into functional neurons. Building on in vitro work, many studies have demonstrated that astrocytes can be transformed into neurons in different disease models to replace damaged or lost cells. However, many findings in this field are controversial, as the source of new neurons has been questioned. This review summarizes progress in reprogramming astrocytes into neurons in vivo in animal models of spinal cord injury, brain injury, Huntington's disease, Parkinson's disease, Alzheimer's disease, and other neurodegenerative conditions.

5.
Mikrochim Acta ; 189(12): 470, 2022 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-36435950

RESUMO

Plasmonic core-satellite nanostructures assembled from simple building blocks have attracted extensive attention since they were reported by the way of DNA-directed assembly in 1998, because of their unique enhanced and synergistic optical properties and widespread potential applications in biosensing, imaging, drug delivery, and diagnostics. In this review, we introduce the synthetic methods of core-satellite nanostructures, emphazising the bottom-up synthesis method, including DNA, molecular, protein, peptide, amino acids, metal ion-assisted assembly, electrostatic adsorption assembly, clicked-to-assembly, and in situ deposition. Than we review and discuss their morphology classification, and summarize influencing factors of morphology. This is followed by overviews on optical properties, including localized surface plasmon resonance, surface-enhanced Raman scattering, surface-enhanced fluorescence and quenching, and applications in the biomedical field. Finally, the challenges and prospects of these kinds of nanostructures are discussed.


Assuntos
Nanoestruturas , Nanoestruturas/química , Ressonância de Plasmônio de Superfície/métodos , Análise Espectral Raman , DNA , Sistemas de Liberação de Medicamentos
6.
JACC Asia ; 2(6): 653-665, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36444328

RESUMO

Elevated concentration of lipoprotein(a) [Lp(a)] is an independent risk factor for atherosclerotic cardiovascular disease, including coronary artery disease, stroke, peripheral artery disease, and so on. Emerging data suggest that Lp(a) contributes to the increased risk for cardiovascular events even in the setting of effective reduction of plasma low-density lipoprotein cholesterol. Nevertheless, puzzling issues exist covering potential genetic factors, Lp(a) assay, possible individuals for analysis, a cutoff point of increased risk, and clinical interventions. In the Chinese population, Lp(a) exhibited a distinctive prevalence and regulated various cardiovascular diseases in specific ways. Hence, it is valuable to clarify the role of Lp(a) in cardiovascular diseases and explore prevention and control measures for the increase in Lp(a) prevalence in the Chinese population. This Beijing Heart Society experts' scientific statement will present the detailed knowledge concerning Lp(a)-related studies combined with Chinese population observations to provide the key points of reference.

8.
World J Gastroenterol ; 28(38): 5573-5588, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36304082

RESUMO

BACKGROUND: Ischemia-reperfusion injury (IRI) is a major risk associated with liver surgery and transplantation, and its pathological mechanism is complex. Interleukin-1 receptor antagonist (IL-1ra) can protect the liver from IRI. However, the regulatory mechanism of IL-1ra expression is still unclear. AIM: To identify the mechanism that could protect the liver in the early stage of IRI. METHODS: To screen the key genes in hepatic IRI, we performed RNA sequencing and gene enrichment analysis on liver tissue from mice with hepatic IRI. Subsequently, we verified the expression and effect of IL-1ra in hepatic IRI. We also used promoter mutagenesis and chromatin immunoprecipitation assay to search for the transcriptional regulatory sites of hypoxia-inducible factor (HIF)-1α. Finally, to explore the protective mechanism of ischemic preconditioning (IP), we examined the expression of HIF-1α and IL-1ra after IP. RESULTS: We identified IL-1ra as a key regulator in hepatic IRI. The expression of IL-1ra was significantly upregulated after hepatic IRI both in vivo and in vitro. Furthermore, we found that HIF-1α regulated Il-1ra transcription in response to hypoxia. Increased HIF-1α accumulation promoted IL-1ra expression, whereas inhibition of HIF-1α exhibited the opposite effect. We also confirmed a predominant role for hypoxia response element in the regulation of Il1ra transcription by HIF-1α activation. Of note, we demonstrated that IP protects against hepatic IRI by inducing IL-1ra expression, which is mediated through HIF-1α. CONCLUSION: We demonstrated that ischemia or hypoxia leads to increased expression of IL-1ra through HIF-1α. Importantly, IP protects the liver from IRI via the HIF-1α-IL-1ra pathway.


Assuntos
Subunidade alfa do Fator 1 Induzível por Hipóxia , Proteína Antagonista do Receptor de Interleucina 1 , Traumatismo por Reperfusão , Animais , Camundongos , Hipóxia/patologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Fígado/patologia , Traumatismo por Reperfusão/patologia
9.
Ann Clin Lab Sci ; 52(4): 556-562, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-36197767

RESUMO

OBJECTIVE: To identify the relation of microvascular density (MVD) to the early postoperative recurrence and metastasis of T1 esophageal squamous cell carcinoma, and to determine whether MVD could be a prognostic predictor of esophageal squamous cell carcinoma. METHODS: Patients with T1 esophageal squamous cell carcinoma were enrolled. Immunohistochemistry with primary antibody against CD-34 was performed to count MVD. ROC curve was plotted and appropriate cutoff value was determined to evaluate the potential power of MVD in predicting early recurrence and metastasis of T1 esophageal squamous cell carcinoma. Survival curves were drawn by the Kaplan-Meier method and significance were tested by the Mantel-Cox test. RESULTS: A total of 37 patients with T1 esophageal squamous cell carcinoma were enrolled. The MVD of T1 esophageal squamous cell carcinoma patients with early metastasis was significantly higher than that of T1 esophageal squamous cell carcinoma patients without early metastasis (65.83±4.39 vs. 42.26±2.34, p<0.001). MVD was available in distinguishing whether patients with early esophageal are prone to postoperative recurrence or metastasis (AUC=0.861; 95% CI 0.738-0.984, p<0.001), with 88.89% sensitivity and 68.42% specificity of MVD being obtained when the cut-off is 44.5. Kaplan-Meier survival curves showed that patients with a higher MVD had a lower survival (37.35 months) compared with those with low MVD (40.79 months) (p<0.05). CONCLUSIONS: MVD could be a promising indicator for early postoperative recurrence and metastasis of T1 esophageal squamous cell carcinoma and the prognosis of these patients.


Assuntos
Neoplasias Esofágicas , Carcinoma de Células Escamosas do Esôfago , Neoplasias Esofágicas/cirurgia , Carcinoma de Células Escamosas do Esôfago/cirurgia , Humanos , Densidade Microvascular , Neovascularização Patológica , Prognóstico
10.
Front Pharmacol ; 13: 953845, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36160427

RESUMO

Proprotein convertase subtilisin kexin type 9 (PCSK9) is a proprotein convertase that increases plasma low-density lipoprotein cholesterol (LDL-C) levels by triggering the degradation of LDL receptors (LDLRs). Beyond the regulation of circulating LDL-C, PCSK9 also has direct atherosclerotic effects on the vascular wall and is associated with coronary plaque inflammation. Interestingly, emerging data show that women have higher circulating PCSK9 concentrations than men, suggesting that the potential roles of PCSK9 may have different impacts according to sex. In this review, we summarize the studies concerning sex difference in circulating levels of PCSK9. In addition, we report on the sex differences in the relations of elevated circulating PCSK9 levels to the severity and prognosis of coronary artery disease, the incidence of type 2 diabetes mellitus, and neurological damage after cardiac arrest and liver injury, as well as inflammatory biomarkers and high-density lipoprotein cholesterol (HDL-C). Moreover, sex difference in the clinical efficacy of PCSK9 inhibitors application are reviewed. Finally, the underlying mechanisms of sex difference in circulating PCSK9 concentrations and the clinical implications are also discussed.

11.
Crit Rev Anal Chem ; : 1-13, 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36094825

RESUMO

Since the colorimetric method has the characteristics of being simple and low cost, the fluorescence spectrum has the characteristics of a strong signal, and Surface-enhanced Raman scattering (SERS) detection has the characteristics of high sensitivity and strong specificity, people usually use these three methods for detection, but the detection of a single sample takes more time. If multiple samples can be tested at the same time, the detection efficiency and sensitivity can be improved, and the selectivity and reliability will be greatly improved. Multiplex sensing also provides a new direction for researchers. To fully understand the research of multiplex sensing based on the plasmonic optics of noble metal nanostructures, this review summarizes all the results previously reported in this field. It also discusses the principles of various detection methods and the biochemical application of multiple detections and finally summarizes the challenges and prospects.

12.
FEBS Open Bio ; 12(10): 1857-1874, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36052535

RESUMO

Stromal antigen 1 (STAG1), a component of cohesion, is overexpressed in various cancers, but it is unclear whether it has a role in the transcriptional regulation of hepatocellular carcinoma (HCC). To test this hypothesis, here, we screened global HCC datasets and performed multiscale embedded gene co-expression network analysis to identify the potential functional modules of differentially expressed STAG1 co-expressed genes. The putative transcriptional targets of STAG1 were identified using chromatin immunoprecipitation followed by high-throughput DNA sequencing. The cohesin-associated gene score (CAGS) was quantified using the The Cancer Genome Atlas HCC cohort and single-sample gene set enrichment analysis. Distinct cohesin-associated gene patterns were identified by calculating the euclidean distance of each patient. We assessed the potential ability of the CAGS in predicting immune checkpoint blockade (ICB) treatment response using IMvigor210 and GSE78220 cohorts. STAG1 was upregulated in 3313 HCC tissue samples compared with 2692 normal liver tissue samples (standard mean difference = 0.54). A total of three cohesin-associated gene patterns were identified, where cluster 2 had a high TP53 mutated rate and a poor survival outcome. Low CAGS predicted a significant survival advantage but presaged poor immunotherapy response. Differentially expressed STAG1 co-expression genes were enriched in the mitotic cell cycle, lymphocyte activation, and blood vessel development. PDS5A and PDGFRA were predicted as the downstream transcriptional targets of STAG1. In summary, STAG1 is significantly upregulated in global HCC tissue samples and may participate in blood vessel development and the mitotic cell cycle. A cohesin-associated gene scoring system may have potential to predict the ICB response.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Carcinoma Hepatocelular/genética , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Proteínas Cromossômicas não Histona , Humanos , Inibidores de Checkpoint Imunológico , Neoplasias Hepáticas/genética
13.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 44(4): 555-562, 2022 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-36065686

RESUMO

Objective To explore the therapeutic effect of ethambutol tablets (EMB) on pulmonary tuberculosis (PTB) in rats and whether the action mechanism of EMB is related to Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway. Methods Sixty SD rats were assigned into a control group,a PTB group,a PTB+EMB group (30 mg/kg),and a PTB+EMB+Colivelin (JAK/STAT pathway activator) group (30 mg/kg+1 mg/kg) via the random number table method,with 15 rats in each group.The rats in other groups except the control group were injected with 0.2 ml of 5 mg/ml Mycobacterium tuberculosis suspension to establish the PTB model.After the modeling,the rats were administrated with corresponding drugs for 4 consecutive weeks (once a day).On days 1,14,and 28 of administration,the body weights of rats were measured and the Mycobacterium tuberculosis colonies were counted.Hematoxylin-eosin staining was carried out to detect the pathological changes in the lung tissue.Enzyme-linked immunosorbent assay was employed to measure the levels of interleukin(IL)-6,tumor necrosis factor-α (TNF-α),IL-1ß,and interferon-γ (IFN-γ) in the serum.Flow cytometry was used to determine the levels of T lymphocyte subsets CD3+,CD4+,CD8+,and CD4+/CD8+.The 16S rRNA sequencing was performed to detect the relative abundance of the intestinal microorganisms.Western blotting was employed to determine the expression of the proteins in the JAK/STAT pathway. Results Compared with the control group,the modeling of PTB reduced the rat body weight (on days 14 and 28),increased Mycobacterium tuberculosis colonies,caused severe pathological changes in the lung tissue,and elevated the levels of IL-6,TNF-α,and IL-1ß in serum and CD8+.Moreover,the modeling increased the relative abundance of Bacteroides,Peptococcus,Clostridium,Actinomyces,Lactobacillus,Verrucomicrobium,and Veillonella in the intestine,up-regulated the protein levels of phosphorylated JAK2 and phosphorylated STAT3 in the lung tissue,and lowered the levels of CD3+,CD4+,CD4+/CD8+,and IFN-γ levels (all P<0.001).Compared with the PTB group,PTB+EMB increased the rat body weight (on days 14 and 28),reduced Mycobacterium tuberculosis colonies,alleviated the pathological damage in lung tissue,lowered the levels of IL-6,TNF-α,and IL-1ß in serum and CD8+.Moreover,the treatment decreased the relative abundance of Bacteroides,Peptococcus,Clostridium,Actinomyces,Lactobacillus,Verrucomicrobium,Veillonella in the intestine,down-regulated the protein levels of phosphorylated JAK2 and phosphorylated STAT3 in the lung tissue,and elevated the levels of CD3+,CD4+,CD4+/CD8+,and IFN-γ (all P<0.001).Colivelin weakened the alleviation effect of EMB on PTB (all P<0.001). Conclusion EMB can inhibit the JAK/STAT signaling pathway to alleviate the PTB in rat.


Assuntos
Mycobacterium tuberculosis , Tuberculose Pulmonar , Animais , Peso Corporal , Etambutol/farmacologia , Interferon gama/metabolismo , Interferon gama/farmacologia , Interleucina-6/metabolismo , Janus Quinases/metabolismo , Janus Quinases/farmacologia , Mycobacterium tuberculosis/metabolismo , RNA Ribossômico 16S , Ratos , Ratos Sprague-Dawley , Fatores de Transcrição STAT/metabolismo , Fatores de Transcrição STAT/farmacologia , Transdução de Sinais , Comprimidos/farmacologia , Tuberculose Pulmonar/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
14.
Hypertens Res ; 45(12): 1882-1890, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36123399

RESUMO

Numerous trials have shown that lowering blood pressure (BP) reduces cardiovascular risk and mortality, yet data about the impact of BP on cardiovascular death risk in patients aged ≥80 years with acute myocardial infarction (AMI) are sparse. This study explored the prognostic value of BP for cardiovascular death during the first 48 h after admission following AMI among patients aged ≥80 years. A total of 1005 patients ≥80 years with AMI were enrolled. Average BP parameters, including systolic, diastolic, and pulse BP, over the first 48 h after admission were calculated. The end point was cardiovascular death. Receiver operating curve (ROC) analysis was used to identify whether BP was relevant to cardiovascular death. The relationship between BP levels and cardiovascular death was evaluated by Cox regression models. ROC analysis showed that average diastolic blood pressure (aDBP), but not systolic and pulse BP, was relevant to cardiovascular death, and the optimal cutoff was 65 mmHg. During the 2.9-year follow-up, patients who died from a cardiovascular cause had lower aDBP levels than those who did not (p = 0.002). Patients with aDBP <65 mmHg had a 1.5-fold higher incidence of cardiovascular death than those with aDBP ≥65 mmHg (35.9% vs. 24.0%; p < 0.001). In multivariable regression analysis, low aDBP remained a strong and independent predictor of cardiovascular death (adjusted hazard ratio 1.907; 95% CI 1.303-2.792). aDBP was independently associated with cardiovascular death in patients aged ≥80 years with AMI, suggesting that aDBP may be a useful index to predict worse outcome in these patients.


Assuntos
Hipertensão , Infarto do Miocárdio , Humanos , Pressão Sanguínea , Estudos Prospectivos , Determinação da Pressão Arterial
15.
Age Ageing ; 51(9)2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36088600

RESUMO

BACKGROUND: the relationship between low-density lipoprotein cholesterol (LDL-C) and adverse outcomes among the older people remains controversial. OBJECTIVE: to further clarify the association between admission LDL-C levels and cardiovascular mortality (CVM) among oldest old individuals (≥80 years) with acute myocardial infarction (AMI). DESIGN: a prospective cohort study. SETTING: two-centre. SUBJECTS: a consecutive sample of 1,224 oldest old individuals with AMI admitted to Beijing FuWai and Shenzhen FuWai hospitals. METHODS: all individuals were subdivided according to baseline LDL-C levels (<1.8, 1.8-2.6 and ≥ 2.6 mmol/l) and further stratified by high-sensitivity C-reactive protein (hsCRP) concentrations (<10 and ≥10 mg/l). The primary outcome was CVM. The time from admission to the occurrence of CVM or the last follow-up was analysed in Kaplan-Meier and Cox analyses. RESULTS: the median age of the overall population was 82 years. During an average of 24.5 months' follow-up, 299 cardiovascular deaths occurred. Kaplan-Meier analysis showed that LDL-C < 1.8 mmol/l group had the highest CVM among oldest old individuals with AMI. Multivariate Cox regression analysis further revealed that compared with those with LDL-C levels <1.8 mmol/l, subjects with LDL-C levels ≥2.6 mmol/l (hazard ratio: 0.67, 95% confidence interval: 0.46-0.98) had significantly lower risk of CVM, especially in those with high hsCRP levels. Moreover, when categorising according to LDL-C and hsCRP together, data showed that individuals with low LDL-C and high hsCRP levels had the highest CVM. CONCLUSIONS: LDL-C < 1.8 mmol/l was associated with a high CVM after AMI in oldest old individuals, especially when combined with high hsCRP levels, which may need to be confirmed by randomised controlled trials.


Assuntos
Proteína C-Reativa , Infarto do Miocárdio , Idoso , Idoso de 80 Anos ou mais , Proteína C-Reativa/metabolismo , LDL-Colesterol , Humanos , Infarto do Miocárdio/diagnóstico , Estudos Prospectivos
16.
Spectrochim Acta A Mol Biomol Spectrosc ; 282: 121714, 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-35940070

RESUMO

In this paper, the fluorescence quenching characteristics of Au-Ag-Pt core-shell nanorods have been studied.Due to nonradiative energy transformation, the fluorescence emission intensity of bovine serum albumin (BSA) could be greatly quenched.It has been found that the quenching effect of Au-Ag-Pt core-shell nanorods could be optimized by adjusting the concentration of chloroplatinic acid.Based on the fluorescence quenching properties of Au-Ag-Pt core-shell nanorods, Au-Ag-Pt trimetal fluorescence quenching nanoprobe has been prepared, and the specificity of alpha-fetoprotein (AFP) detection has also been realized.In order to guarantee the sensing specificity, the surface modification including carboxyl replacement, carboxyl activation and antibody connection have been performed on Au-Ag-Pt core-shell nanorods.By using the principle of specific combination of antigen and antibody, the specific detection of AFP has been realized with a lower detection limit of 4.0 pg/mL, and the linear detection range spans a scope from 0.03 to 0.5 ng/mL.Interference experiments and the actual samples detection results show that the Au-Ag-Pt trimetal core-shell nanorod probes have good anti-interference and repeatability.


Assuntos
Nanotubos , alfa-Fetoproteínas , Fluorescência , Ouro , Prata
17.
Huan Jing Ke Xue ; 43(8): 3966-3976, 2022 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-35971695

RESUMO

The ozone concentration characteristics of 13 cities in Beijing-Tianjin-Hebei regions from 2016 to 2020 were analyzed based on ecological environment monitoring and meteorological observation data. The influence of meteorological elements such as daily maximum temperature (Tmax), daily average ground pressure (p), daily average ground relative humidity (RH), and daily average ground wind speed (v) on ozone concentration[ρ(O3-8h)] and the exceeding standard rate of O3-8h were discussed. The AQI, ozone concentration range, and ozone pollution level forecast accuracy rates were evaluated using the neural network statistical model. The results showed that the concentrations of O3-8h-90per[ρ(O3-8h-90per)] of 13 cities in the Beijing-Tianjin-Hebei region from 2016 to 2020 were 157.4, 177.2, 177.3, 190.6, and 175.6 µg·m-3, respectively. The regional ozone concentration increased by 11.6% over the five years from 2016 to 2019. From 2016 to 2019, there was an overall upward trend in volatility, followed by a decline in 2020. Compared with that in 2016, the concentration of O3-8h-90per in the other 10 cities increased by 6-45.5 µg·m-3, except for in Beijing, Zhangjiakou, and Chengde, where it decreased slightly. The average value of ρ(O3-8h) from April to September was higher than 100 µg·m-3, and the highest monthly average concentration of O3-8h was 158.10 µg·m-3 in June. The range of the over standard rate of O3-8h was 8.6%-19.2% in the 13 cities, and 97.8% of ozone concentrations exceeded the standard in the period from April to September. At the regional scale, the concentration of O3-8h had the strongest correlation with the daily maximum temperature. Furthermore, when Tmax was in the range of 25-28℃, the concentration of O3-8h in the 13 cities began to exceed the standard concentration of 160 µg·m-3. Additionally, the concentration of O3-8h negatively correlated with p. When RH was below 60%, ozone concentration increased slowly with relative humidity in most cities. When RH was above 61%-70%, ozone concentration decreased with the increase in daily relative humidity in most cities. When ozone exceeded the standard concentration of 160 µg·m-3, the dominant wind was mainly southerly wind, and the high ozone concentration in most cities tended to be concentrated in the low wind speed range of 2-3 m·s-1 and below. Moreover, the correlation coefficient range of the statistical model of OPAQ 1-9 days in advance was 0.72-0.86, the average accuracy of AQI level forecasts was 67%-86%, and the average accuracy of O3-8h concentration forecasts was 63%-84%. In April to September, when ozone exceeded the standard of 160 µg·m-3, the accuracy rates of the model forecast of light ozone pollution and ozone exceeding the standard concentration of 160 µg·m-3three days in advance were 69% and 66%, which can provide a reference for the management and control of ozone pollution.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Ozônio , Poluentes Atmosféricos/análise , Poluição do Ar/análise , Pequim , China , Cidades , Monitoramento Ambiental/métodos , Redes Neurais de Computação , Ozônio/análise , Material Particulado/análise , Estações do Ano
18.
J Hypertens ; 40(9): 1713-1721, 2022 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-35943100

RESUMO

BACKGROUND: Hypertension is a known risk factor for cardiovascular disease; however, its impact on clinical outcomes in patients with heterozygous familial hypercholesterolemia (HeFH) is unclear. Hence, we aimed to investigate the effects of hypertension on severity of coronary artery atherosclerosis and cardiovascular outcomes in patients with HeFH. METHODS: A total of 480 patients with clinical or molecular diagnosis of definite or probable familial hypercholesterolemia according to Dutch Lipid Clinic Network criteria (DLCN score ≥6) were included in the study. They were divided into the two groups according to their blood pressure status: hypertension group and normotension group. The severity of coronary stenosis was assessed by a number of diseased vessels, Gensini, Syntax, and Jeopardy scores. All individuals were followed up for cardiovascular events (CVEs) and cox proportion hazard models were used to evaluate the association of hypertension with cardiovascular outcomes. RESULTS: Patients with hypertension had more severe coronary stenosis and a higher incidence of CVEs compared with the ones with normotension (log-rank P < 0.001). After multivariable adjustment, there was a 2.1-fold increased risk of CVEs among patients with hypertension compared with patients with normotension (adjusted hazard ratio 2.06, 95% confidential interval 1.17-3.65, P < 0.01). Hypertension control status was also associated with CVEs even after adjustment of multiple variables. However, no combined effect on increased cardiovascular risks was detected in this HeFH cohort. CONCLUSION: In patients with HeFH, hypertension is an independent risk factor for cardiovascular events. Moreover, blood pressure control status in patients with hypertension is associated with the worse outcomes.


Assuntos
Doença da Artéria Coronariana , Estenose Coronária , Hiperlipoproteinemia Tipo II , Hipertensão , Doença da Artéria Coronariana/complicações , Humanos , Hiperlipoproteinemia Tipo II/complicações , Hiperlipoproteinemia Tipo II/diagnóstico , Hipertensão/complicações , Fatores de Risco
19.
Exp Neurol ; 358: 114208, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35988700

RESUMO

Hypertension is a confirmed risk factor for cerebral hemorrhage in humans. Which endogenous factor directly induces hypertension-related hemorrhage is unclear. In this study, 42 hemorrhagic patients with hypertension and hyperlipidemia and 42 age-matched healthy controls were enrolled. The contents of serum semicarbazide-sensitive amine oxidase (SSAO) and formic acid (FC, FC is a final product of SSAO through the oxidation of endogenous formaldehyde, which results from the enzymatic oxidative deamination of the SSAO substrate, methylamine) were examined in the patients after stroke. Hemorrhagic areas were quantified by computer tomography. In the animal study, hemorrhagic degree was assessed by hemotoxylin & eosin or tissue hemoglobin kits. The relationship between FC and blood pressure/hemorrhagic degree was examined in wild-type mice and hSSAOTG mice fed with high-fat diets or high-fat and -salt diets. The results showed that the levels of serum FC were positively correlated with blood pressure and hemorrhagic areas in hemorrhagic patients. Transfection of microRNA-134 could enhance SSAO expression in human vascular smooth muscle cells. Consistently, after treatment with high-fat and -salt diets, hSSAOTG mice exhibited higher levels of miR134 and FC, higher blood pressure, and more severe hemorrhage than wild-type mice. Interestingly, folic acid reduced hypertension and hemorrhage in hSSAOTG mice fed with high-fat diets. These findings suggest that FC is a crucial endogenous factor for hypertension and hemorrhage.


Assuntos
Amina Oxidase (contendo Cobre) , Hipertensão , MicroRNAs , Amina Oxidase (contendo Cobre)/metabolismo , Amina Oxidase (contendo Cobre)/farmacologia , Animais , Amarelo de Eosina-(YS) , Ácido Fólico , Formaldeído/farmacologia , Formiatos , Hematoxilina , Hemorragia , Humanos , Metilaminas/metabolismo , Camundongos
20.
Angiology ; : 33197221121191, 2022 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-35993693

RESUMO

Acute myocardial infarction (AMI) is a leading cause of morbidity and mortality worldwide and it is primarily caused by acute plaque disruption and coronary occlusion. Recent studies suggest that myocardial infarction with non-obstructive coronary arteries (MINOCA) also occurs but the underlying mechanisms have not been fully understood until recently. The evidence also suggests that the clinical outcomes of patients presenting with MINOCA are similar to AMI patients with obstructive coronary artery disease (MI-CAD), including all-cause mortality and major adverse cardiovascular events. The present narrative review considers the risk factors, pathological changes, and outcomes associated with MINOCA and compares them with MI-CAD.

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