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1.
J Cell Physiol ; 236(2): 1391-1400, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32749682

RESUMO

The development of primordial germ cells (PGCs) undergoes epigenetic modifications. The study of histone methylation in regulating PGCs is beneficial to understand the development and differentiation mechanism of germ stem cells. Notably, it provides a theoretical basis for directed induction and mass acquisition in vitro. However, little is known about the regulation of PGC formation by histone methylation. Here, we found the high enrichment of H3K4me2 in the blastoderm, genital ridges, and testis. Chromatin immunoprecipitation sequencing was performed and the results revealed that genomic H3K4me2 is dynamic in embryonic stem cells, PGCs, and spermatogonial stem cells. This trend was consistent with the H3K4me2 enrichment in the gene promoter region. Additionally, narrow region triggered PGC-related genes (Bmp4, Wnt5a, and Tcf7l2) and signaling pathways (Wnt and transforming growth factor-ß). After knocking down histone methylase Mll2 in vitro and vivo, the level of H3K4me2 decreased, inhibiting Cvh and Blimp1 expression, then repressing the formation of PGCs. Taken together, our study revealed the whole genome map of H3K4me2 in the formation of PGCs, contributing to improve the epigenetic study in PGC formation and providing materials for bird gene editing and rescue of endangered birds.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33220395

RESUMO

PURPOSE: This study aimed to compare erlotinib (E) and etoposide/cisplatin (EP) with concurrent radiotherapy (RT) for patients with stage IIIA/B unresectable advanced non-small-cell lung cancer (NSCLC) with activating epidermal growth factor receptor mutation (EGFRm+). METHODS: and patients: This was a multicenter, randomized, open-label, phase 2 trial conducted across 19 institutions in China (December 2012 to January 2016). Enrolled patients were randomized (1:1) to E+RT (oral erlotinib 150 mg/day for 2 years or until disease progression or intolerable toxicity and RT 200 cGy/day, 5 days/week for 6 weeks from the first day of erlotinib) or EP+RT (etoposide 50 mg/m2 intravenously on days 1-5 and 29-33; cisplatin 50 mg/m2 intravenously on days 1, 8, 29 and 36; and RT as above). The primary endpoint was progression-free survival (PFS). Secondary endpoints included objective response rate (ORR) and safety. RESULTS: A total of 252 patients were screened and 20 patients with EGFRm+ in each group received the allocated E+RT or EP+RT treatment. Patient characteristics were well-balanced between groups. Compared with EP+RT, median PFS with E+RT was significantly longer (24.5 vs 9.0 months [hazard ratio, 0.104; 95% confidence interval, 0.028-0.389; P < 0.001]). ORR in the E+RT and EP+RT groups was 70% and 61.9%, respectively (P = 0.744). The incidence of adverse events (any grade) was similar between E+RT and EP+RT groups (88.9% and 84.2%). CONCLUSIONS: The primary endpoint of PFS was met, and the data showed that E+RT might provide PFS improvement compared with EP+RT, with similar tolerability. However, definitive statements regarding the efficacy of concurrent E+RT in patients with unresectable stage III NSCLC with activating EGFRm+ cannot be made, and slow patient accrual will likely make it infeasible to conduct a phase 3 study.

3.
Transl Oncol ; 14(1): 100956, 2020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-33227662

RESUMO

OBJECTIVE: There is no consensus on the optimal timing of postoperative radiotherapy (PORT) for locally advanced esophageal squamous cell carcinoma (ESCC). We aimed to determine whether the timing of PORT affects the long-term prognosis of ESCC, and plotted nomograms to predict survival. METHODS: We retrospectively analyzed 351 ESCC patients who underwent radical surgery and PORT. Receiver operating characteristic curves were used to estimate the optimal cutoff point of the time interval between surgery and PORT. Cox proportional hazards regression was used to identify prognostic predictors. Overall survival (OS) and progression-free survival (PFS) were predicted using nomograms. RESULTS: The median follow-up was 53 months (range: 3-179 months). Compared to early PORT, PORT at >48 days after surgery was associated with better OS (adjusted hazard ratio [HR]: 1.406, p = 0.037) and PFS (adjusted HR: 1.475, p = 0.018). In the chemotherapy subgroup, incorporation of chemotherapy timing into the analysis suggested that 2-4 chemotherapy cycles followed by PORT was the optimal treatment schedule as compared to 0-1 chemotherapy cycle followed by PORT and concurrent chemoradiotherapy (5-year PFS: 65.9% vs. 51.0% vs. 50.1%; p = 0.049). The nomograms for OS and PFS were superior to the TNM classification (concordance indices: 0.721 vs. 0.626 and 0.716 vs. 0.610, respectively). CONCLUSIONS: Delayed PORT (>48 days) provides better survival benefit than early PORT among ESCC patients. PORT following 2-4 chemotherapy cycles might lead to the best survival rate. The nomogram plotted in this study effectively predicted survival and may help guide treatment.

4.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(6): 814-821, 2020 Jun 30.
Artigo em Chinês | MEDLINE | ID: mdl-32895207

RESUMO

OBJECTIVE: To evaluate the effect of using free double- leaf perforator flap posterolateral calf peroneal artery in anatomical reconstruction of the oropharyngeal structure after ablation of advanced oropharyngeal carcinoma. METHODS: Twenty-six patients with oropharyngeal defects after ablation of oropharyngeal malignancies were recruited, including 12 with carcinoma in the tongue base, 5 in the latenral pharyngeal wall and 9 in the soft palate. Between July, 2016 and July, 2018, the patients underwent surgeries for reconstruction of the oropharyngeal defects using flaps. The areas of tissue defects repaired by double-leaf perforator flaps ranged from 40.5 to 72.5 cm2. Reconstruction was performed for oropharyngeal defects in the soft palate, pterygopalate, parapharyngeal, pterygo- mandibular, and tongue base tissues. The patients' outcomes including mouth opening, functions of deglutition, linguistic function, restoration of palatopharyngeal anatomical structure and postoperative survival were evaluated, and their quality of life was assessed using FACT-H&N scale (Chinese Edition). RESULTS: All the 26 patients with transplantation of the free flaps survived. Six months after the operation, the oropharyngeal function and anatomical structure of the patients were basically restored. The questionnaire survey showed that the patients' physical, social/family, emotional and functional conditions, the total score of the core scale, items scores for the head and neck, and the total score of the scale all improved significantly after the operation compared with those before the operation (P < 0.05). CONCLUSIONS: The free peroneal artery bilobate perforator flap in the posterolateral crus, which seldom has anatomical variations of the blood vessels, allows flexible design and contains rich tissue volume to facilitate defect repair with different approaches and ranges. The application of this flap, which is an ideal perforator flap for reconstruction of the oropharyngeal structure and function, can improve the quality of life of patients following operations for advanced oropharyngeal cancer.


Assuntos
Retalhos de Tecido Biológico , Neoplasias Orofaríngeas , Retalho Perfurante , Artérias , Humanos , Qualidade de Vida , Procedimentos Cirúrgicos Reconstrutivos , Transplante de Pele , Lesões dos Tecidos Moles
5.
Mol Med ; 26(1): 88, 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32942985

RESUMO

BACKGROUND: Patients newly diagnosed with lung adenocarcinoma with bone metastases (LABM) have poor survival rates after treatment with conventional therapies. To improve outcomes, we retrospectively investigated whether the application of a more comprehensive genetic test of tumor biopsies samples from LABM patients could provide the basis for treatment with more effective tyrosine kinase inhibitors (TKIs) regimens. METHODS: Fine needle biopsies were taken from the primary tumor (PT) and a secondary bone metastasis (BM) of 17 LABM patients before treatment. Simple genetic profiles for selecting therapies were initially obtained using an ARMS-PCR test for EGFR and ALK fusion mutations. More detailed genetic profiles of somatic exon SNVs and CNVs in 457 cancer-related genes were retrospectively derived using capture single molecule amplification and resequencing technology (capSMART). RESULTS: ARMS-PCR identified 14 EGFR positive, 3 EGFR negative and 1 ALK fusion positive patient. A therapy regimen incorporating TKIs Gefitinib and Crizotinib was offered to the EGFR and ALK fusion positive patients, respectively. With the exception of two patients, molecular profiling of matching PT and BM biopsies identified a highly shared somatic variant fingerprint, although the BMs exhibited additional genomic instability. In six of 13 EGFR positive patients and in all three EGFR negative patients, examination of the genetic profiles identified additional clinically significant mutations that are known or experimental drug targets for treatment of lung cancer. CONCLUSION: Our findings firstly suggest that treatment regimens based on comprehensive genetic assessment of newly diagnosed LABM patients should target both the PT and secondary BMs, including rogue clones with potential to form new BMs. Second, the additional information gained should allow clinicians to design and implement more personalized treatment regimens and potentially improve outcomes for LABM patients.

6.
Artigo em Chinês | MEDLINE | ID: mdl-32842209

RESUMO

Objective:To investigate the clinical application of free skin flap in the one-stage repair and reconstruction after total glossectomy in patients with advanced tongue cancer. Method:Twenty-eight patients who underwent total glossectomy for advanced tongue cancer and treated with the one-stage repair and reconstruction with free skin flap ranged from January 2008 to July 2018 were recruited in this study. Among them, there were 12 cases of squamous cell carcinoma, 8 cases of adenocarcinoma, and 8 cases of adenoid cystic carcinoma. The free radical forearm flap and perforator flap of posterolateral peroneal calf peroneal artery were used in 16 and 12 cases, respectively. All patients were treated with outpatient functional rehabilitation sequence and postoperative radiotherapy within 6 months, and were followed up regularly for 1, 3 and 5 years to understand their living conditions, and Washington University students' quality questionnaire 4 was used to investigate the functional factors of the healthy patients in different periods of time after surgery. Result:The survival rate of the free skin flap was 94%. The radical forearm flap was immediately changed to perforator flap posterolateral peroneal calf peroneal artery in 2 cases due to the necrosis of the artery. The hospitalization time of the patients was 10-14 days, with an average of 12.5 days. The wounds inside and outside of the mouth were healed by first intention at the time of discharge. All nasogastric tube and tracheal tube were removed safely within 45 days after the surgery and the functions of swallowing, breathing, language and chewing were gradually restored. According to the questionnaire survey, patients' attention to quality of life gradually shifted from function to feeling. With the direct calculation method, the 1-,3-, and 5-year survival rate was 96.40%, 64.71%, 55.60%, respectively, while tumor-free survival rate was 85.70%, 58.82%, 55.60%, respectively. Conclusion:The comprehensive treatment based on free tissue flap repair and reconstruction of tissue defects can significantly improve the radical cure rate of patients with advanced tongue cancer, and can effectively restore the functions of swallowing, breathing, language, chewing and so on. It is a kind of treatment method worthy of promotion.


Assuntos
Retalhos de Tecido Biológico , Retalho Perfurante , Procedimentos Cirúrgicos Reconstrutivos , Neoplasias da Língua/cirurgia , Glossectomia , Humanos , Qualidade de Vida , Transplante de Pele
7.
Cancer Manag Res ; 12: 6541-6551, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801892

RESUMO

Background: This study aimed to analyze the risk factors for esophageal squamous cell carcinoma (ESCC), especially extracapsular lymph node involving the esophagus (ECLNIE), occurring during or after radiotherapy (RT) in patients with esophageal perforation (EP). Methods: In total, 306 patients with ESCC who received RT and/or chemotherapy between January 2016 and December 2017 in our hospital and who met the inclusion criteria of the study were recruited. The continuous variables were converted into classification variables using the receiver operating characteristic curve or common clinical parameters. Risk factors for EP were examined by univariable analysis using the chi-square test or Fisher's exact and by multivariable analysis using logistic regression model. Propensity score matching (PSM) was used to compensate for the differences in baseline characteristics, and the incidence of EP was compared after matching. Results: EP was observed in 26 patients (incidence rate, 8.5%). Univariable analysis revealed that age, BMI, T4 stage, tumor length, esophageal wall thickness, ECLNIE, necrotic areas, niche sign by esophagogram before RT, neutrophil-to-lymphocyte ratio, and prognostic nutritional index were significantly associated with EP among patients with ESCC who received radiotherapy. Multivariable analysis demonstrated that age, ECLNIE, esophageal wall thickness, and niche sign by esophagogram before RT were independent risk factors for EP. After PSM, compared with patients without ECLNIE, patients with ESCC and ECLNIE had a significantly higher risk of EP. Conclusion: The presence of ECLNIE could be a strong risk factor of EP during and after RT.

8.
Insect Biochem Mol Biol ; 125: 103439, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32712075

RESUMO

Moths often use multi-component pheromones with fixed ratios to keep intraspecific communication and interspecific isolation. Unusually, the Oriental armyworm Mythimna separata in North China use only Z11-16:Ald as the essential component of its sex pheromone to find mates. To understand how this species keeps behavioral isolation from other species sharing Z11-16:Ald as a major pheromone component, we study the olfactory coding of intra- and interspecific pheromonal messages in the males of M. separata. Firstly, we functionally characterized the long trichoid sensilla in male antennae by single sensillum recording. Two types of sensilla were classified: the A type sensilla responded to Z11-16:Ald and Z9-14:Ald, and the B type sensilla mainly to Z9-14:Ald, and also to Z11-16:Ac, Z11-16:OH, and Z9-16:Ald. Next, we examined the glomerulus responses in the antennal lobes to these compounds by using in vivo optical imaging. The results showed that among the three subunits of the macroglomerular complex (MGC), Z11-16:Ald activated the cumulus, Z9-14:Ald activated the dorso-anterior and the cumulus, Z11-16:OH and Z11-16:Ac activated the dorso-anterior and dorso-posterior, respectively. However, Z9-16:Ald activated an ordinary glomerulus. Thirdly, we tested the behavioral responses of the males to these compounds in the wind tunnel. Addition of Z9-14:Ald at the ratio of 1:10 greatly reduced the attractiveness of Z11-16:Ald, addition of Z9-16:Ald or Z11-16:OH at the ratio of 1:1 also had behavioral antagonistic effects, while addition of Z11-16:Ac had no effect on the attractiveness of Z11-16:Ald. Finally, we used antennal transcriptome data and the Xenopus expression system to identify the receptor of Z9-14:Ald in M. separata. The Xenopus oocytes co-expressing MsepOR2 and MsepORco showed a strong response to Z9-14:Ald. Two-color fluorescence in situ hybridization validated that the cells expressing MsepOR2 and MsepOR3, tuned to Z9-14:Ald and Z11-16:Ald respectively, were localized in the different sensilla of male antennae. Comparing the sex pheromone communication channel of the related species, our results suggest that the conserved olfactory pathways for behavioral antagonists play a crucial role in behavioral isolation of noctuid species.

9.
Inorg Chem ; 2020 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-32489095

RESUMO

The silicon atom in LSiCl or LSiMes (L = PhC(NtBu)2, Mes = 2,4,6-Me3C6H2) inserts into the B-X bond of RBX2 (R = Ph, Mes, N(SiMe3)2; X = Cl, Br), which is followed by the migration of the amidinate ligand and the halide atom. By this way, LB(R)SiCl3 (R = Ph, 2; Mes, 3; N(SiMe3)2, 4) and LB(R)SiX2Mes (R = Ph, X = Cl, 5; R = Mes, X = Cl, 6; R = Mes, X = Br, 7) were obtained. Furthermore, a silylene-borane adduct LClSi → BPhCl2 (1) was obtained as an intermediate in the formation of compound 2. Compounds 2-7 are rare examples of borylhalosilanes.

10.
Genomics Proteomics Bioinformatics ; 18(1): 81-90, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32428603

RESUMO

Accurate detection of low frequency mutations from plasma cell-free DNA in blood using targeted next generation sequencing technology has shown promising benefits in clinical settings. Duplex sequencing technology is the most commonly used approach in liquid biopsies. Unique molecular identifiers are attached to each double-stranded DNA template, followed by production of low-error consensus sequences to detect low frequency variants. However, high sequencing costs have hindered application of this approach in clinical practice. Here, we have developed an improved duplex sequencing approach called SinoDuplex, which utilizes a pool of adapters containing pre-defined barcode sequences to generate far fewer barcode combinations than with random sequences, and implemented a novel computational analysis algorithm to generate duplex consensus sequences more precisely. SinoDuplex increased the output of duplex sequencing technology, making it more cost-effective. We evaluated our approach using reference standard samples and cell-free DNA samples from lung cancer patients. Our results showed that SinoDuplex has high sensitivity and specificity in detecting very low allele frequency mutations. The source code for SinoDuplex is freely available at https://github.com/SinOncology/sinoduplex.


Assuntos
Ácidos Nucleicos Livres/sangue , DNA Tumoral Circulante/sangue , Análise Mutacional de DNA/métodos , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/genética , Software , Algoritmos , Linhagem Celular , Humanos , Mutação , Sensibilidade e Especificidade
11.
Ann Palliat Med ; 9(3): 788-794, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32389008

RESUMO

BACKGROUND: This study was conducted to explore the lymph node metastasis (LNM) pattern of thoracic esophageal cancer (TEC) depending upon the location of the primary tumor and provide a reference for the design of clinical target volume (CTV). METHODS: The data of patients who underwent radical esophagectomy and three-field lymph node dissection at Fujian Cancer Hospital from 2006 to 2010 were retrospectively analyzed. We segmented the esophagus according to the anatomical landmarks on computed tomography (CT) and defined the transsegmental and mono-segmental esophageal carcinoma. The LNM pattern in trans-segmental and monosegmental esophageal cancer was explored and the CTV delineation was determined based on LNM pattern. RESULTS: A total of 852 patients were included in this study. The top five sites of LNM for upper-middle TEC were cervical, upper and middle paraesophageal, and zone 1, 2, 4 regions. The most common sites of LNM for lower-middle TEC were cervical and middle paraesophageal, group 3, 7, and zone 7 regions. The top five sites of LNM for middle-upper TEC were cervical, middle paraesophageal, zone 1, 7, and group 7 regions. The most common sites of LNM for middle-lower TEC were cervical, middle paraesophageal, zone 7, and group 2, 7 regions. The top five sites of LNM for TEC involving all the segments were cervical, middle paraesophageal, zone 7, group 2 and 7 regions. CONCLUSIONS: LNM pattern of trans-segmental and mono-segmental TEC varies depending upon the primary tumor location. The irradiation field must be designed according to the primary tumor location.

12.
Artigo em Inglês | MEDLINE | ID: mdl-32160361

RESUMO

Reaction of the aluminum dialkynyl LAl(CCR)2 (L=N,N-chelate ligand and R=organic group) with B(C6 F5 )3 proceeds through an intermediate with Al⋅⋅⋅η2 -C≡C side-on coordination to form the alumoles (2, 4, 6). A distinctive reaction pattern indicates a new facile synthetic route to aluminum-containing heterocycles. The synthetic process is described, and the characterization of compounds and computational calculations were carried out. Furthermore, alumoles 2 and 4 exhibit an aggregation-induced emission (AIE) of the bright yellow fluorescence.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31972090

RESUMO

Boron particles with a homogeneous carbon-coating layer were employed as the precursor to fabricate MgB2 superconductors to generate artificial two-dimensional (2D) flux-pinning centers. Systematic microstructure investigation reveals that the carbon layers are well-distributed in the MgB2 matrix without agglomeration. The thickness of the carbon layers is smaller than the MgB2 coherent length, which makes them transparent to supercurrent. The critical current density is increased because of the strong flux-pinning effects of the 2D carbon layers in the superconductor as highly efficient flux-pinning centers and the increased irreversibility field due to the carbon-doping effects.

14.
Small ; : e1903418, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31782918

RESUMO

Lithium-ion batteries (LIBs) are widely used in electric vehicles and portable electronic devices due to their high energy density, long cycle life, environmental friendliness, and negligible memory effect, though they also suffer from low power density, safety issues, and an aging effect. Cobalt chalcogenides/phosphides as promising anode materials have attracted intensive interests due to their high theoretical capacity based on the conversion mechanism. Cobaltates (XCo2 O4 , X = the other metal) have attracted attention because the X element can partially replace the high cost and toxic cobalt element. The serious volume variation during the cycling process has an impact, however, on the lithiation environment of above materials. Hierarchical construction can provide more active sites and shorten the diffusion pathways of Li ions as well as accommodating the volume expansion during lithiation processes. Herein, the research progress on the synthesis methods, structural characteristics, and electrochemical performances of cobalt chalcogenides/cobalt phosphides/cobaltates with hierarchical nanostructures for LIBs is presented. The concluding remarks highlight the research challenges and possible development directions of cobalt chalcogenides/cobalt phosphides/cobaltates with tailored hierarchical nanostructures for LIBs.

15.
EBioMedicine ; 49: 133-144, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31668880

RESUMO

BACKGROUND: Active immunotherapy is an effective, long-lasting, cheap, and safe approach to suppress cancer progression; however, the key issue is to develop appropriate tumour vaccines. Oncoproteins are up-regulated under various stress conditions and promote cell survival. Oncoproteins and their immunogenic domains could serve well as tumour vaccines and prime the hosts' active anti-tumour immunity. METHODS: Proteomic and bioinformatic analyses were performed to identify potential tumour associated antigens (TAAs). Then, peptides derived from CD151 were designed and synthesized according to the major histocompatibility complex (MHC) I binding and immunogenicity. Cytotoxicity assay, flow cytometry, immunohistochemistry, and in vivo bioluminescence imaging were performed to assess the active anti-tumour immunity triggered by CD151 peptides in H22 primary hepatoma and experimental 4T1 breast cancer lung metastasis models. FINDINGS: CD151 was identified as an ideal TAA based on proteomic and bioinformatic analyses. CD151 peptides as tumour vaccines triggered active anti-tumour immunity against H22 hepatoma and the lung metastasis of 4T1 breast cancer in two mouse models through the activation of CD8+IFNγ+ lymphocytes and the subsequent targeted cytotoxicity. Further, the peptides suppressed the negative regulators, myeloid-derived suppressor cells. Survival was prolonged for mice with lung metastases from CD151 peptide-immunised groups. INTERPRETATION: The up-regulated oncoproteins in 8 Gy-irradiated tumour cells are good candidates for designing immunogenic peptides as tumour vaccines. Anti-tumour active immunity primed by peptides from CD151 may be an effective and safe approach to suppress cancer progression.


Assuntos
Imunidade Ativa , Neoplasias Pulmonares/imunologia , Neoplasias Pulmonares/secundário , Proteínas Oncogênicas/química , Peptídeos/uso terapêutico , Tetraspanina 24/química , Animais , Antígenos de Neoplasias/metabolismo , Linfócitos T CD8-Positivos/imunologia , Vacinas Anticâncer/imunologia , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Humanos , Imunoterapia , Neoplasias Pulmonares/patologia , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos ICR , Células Supressoras Mieloides/metabolismo , Vacinas de Subunidades/imunologia
16.
BMC Cancer ; 19(1): 1023, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666026

RESUMO

BACKGROUND: The aim of the present study was to assess the efficacy of postoperative chemoradiotherapy (POCRT) following surgery in non-small-cell lung cancer patients with N2 lymph node metastasis (N2-NSCLC). METHODS: The clinical data of patients with N2-NSCLC treated with POCRT or postoperative chemotherapy (pCT) alone were retrospectively collected and reviewed. The overall survival (OS) rates were analyzed utilizing the Kaplan-Meier method and compared by the log-rank test. Cox regression analysis was used to determine factors significantly associated with survival. Propensity score matching (PSM) analysis was used to compensate for differences in baseline characteristics and OS was compared after matching. RESULTS: Between 2004 and 2014, a total of 175 patients fulfilled the inclusion criteria, 60 of whom were treated with POCRT, while 115 were administered pCT. The 1, 3 and 5-year OS rates in the POCRT and pCT groups were 98.3 vs. 86.1%, 71.7 vs. 53.0% and 45.7 vs. 39.0%, respectively (P = 0.019). Compared with pCT, POCRT improved OS in patients with squamous cell subtype (P = 0.010), no lymphovascular invasion (P = 0.006), pN2a (P = 0.006) or total number of metastatic lymph nodes ≤7 (P = 0.016). After PSM, these survival differences between POCRT and pCT remained significant in patients with squamous cell lung cancer (P = 0.010). CONCLUSIONS: POCRT following complete resection may be beneficial for patients with squamous cell lung cancer, particularly those with limited nodal involvement.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma de Células Escamosas/tratamento farmacológico , Quimiorradioterapia Adjuvante , Quimioterapia Adjuvante , Neoplasias Pulmonares/tratamento farmacológico , Metástase Linfática/patologia , Adulto , Idoso , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma Pulmonar de Células não Pequenas/cirurgia , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/cirurgia , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/cirurgia , Linfonodos/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Período Pós-Operatório , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do Tratamento
17.
Medicine (Baltimore) ; 98(42): e17637, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31626150

RESUMO

The purpose of this study was to assess the impact of tumor burden on the survival of patients with pathologic T3N0M0 (pT3N0M0) esophageal squamous cell carcinoma (ESCC).A total of 84 patients with pathologic T3N0M0 ESCC treated with radical esophagectomy and 3-field lymphadenectomy (3-FL) from January 2008 to December 2009 in our center were analyzed. Receiver-operating characteristic (ROC) curve analysis was performed to calculate the optimal cutoff value. The Kaplan-Meier method and log-rank test were used to assess the overall survival (OS) differences between groups. A regression model was applied to identify prognostic factors for OS. Propensity score matching (PSM) was performed to adjust for the imbalance and indication biases in the 2 groups.The median follow-up time was 62 months (range, 1-84 months), and the 5-year OS rate was 62% (95% confidence interval, 52.2-71.8%). According to the ROC curve analysis, the optimal cutoff values for the maximal esophageal wall thickness, tumor length, and tumor volume were 1.3 cm, 5.9 cm, and 18.6 cc, respectively. Univariate analysis revealed that maximal esophageal wall thickness >1.3 cm (P = .014), tumor volume >18.6 cc (P < .001), and vascular invasion (P < .001) were significantly associated with OS. The multivariate Cox regression model identified tumor volume and vascular invasion as factors affecting OS. After propensity matching, patients with a tumor volume ≤18.6 cc had a better OS than those with a tumor volume >18.6 cc (5-year OS, 85% vs 50%, P = .008).Tumor volume may serve as a good prognostic factor for patients with pT3N0M0 ESCC treated with radical esophagectomy and 3-FL. Larger-scale studies are warranted to validate these findings.


Assuntos
Carcinoma de Células Escamosas do Esôfago/diagnóstico , Estadiamento de Neoplasias/métodos , Pontuação de Propensão , Carga Tumoral , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Carcinoma de Células Escamosas do Esôfago/epidemiologia , Carcinoma de Células Escamosas do Esôfago/cirurgia , Esofagectomia , Feminino , Seguimentos , Humanos , Imageamento Tridimensional , Incidência , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Taxa de Sobrevida/tendências , Fatores de Tempo , Tomografia Computadorizada por Raios X
18.
Anal Bioanal Chem ; 411(25): 6755-6765, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31388716

RESUMO

Diphacinone (DPN) is an extensively used anticoagulant rodenticide that is also considered a hazardous chemical, which poses a threat to nontarget species. DPN poisoning cases in humans or other species frequently occur, while rapid and sensitive detection methods are rarely reported. Thus, it is meaningful to develop an immunoassay for DPN detection with high sensitivity and specificity. In this study, a hapten was synthesized and then conjugated with carrier proteins to prepare the immunogens with different conjugation ratios for the preparation of antibody. After evaluation of the antisera using an indirect competitive enzyme-linked immunosorbent assay (icELISA) and statistical analysis, we found that the immunogen prepared using the N,N-dicyclohexylcarbodiimide (DCC) method with a conjugation ratio of 28.5 could elicit mice to generate antibodies with high performance. Using hybridoma technology, we obtained the specific monoclonal antibody (mAb) 4G5 with a half maximal inhibitory concentration (IC50) of 0.82 ng/mL in buffer solution. We initially explored the recognition mechanism of DPN/CLDPN and mAb from both conformational and electronic aspects. Then, mAb 4G5 was applied to develop icELISA for biological samples. The limits of detection (LODs) of icELISA were 0.28 µg/L, 0.32 µg/L, and 0.55 µg/kg for swine plasma, urine, and liver samples, respectively, and the recoveries ranged from 72.3 to 103.3% with a coefficient of variation (CV) of less than 12.3% in spiked samples. In summary, we developed a sensitive, specific, and accurate icELISA for the detection of DPN in biological samples, which showed potential in food safety analysis and clinical diagnosis. Graphical abstract.


Assuntos
Anticoagulantes/análise , Ensaio de Imunoadsorção Enzimática/métodos , Fenindiona/análogos & derivados , Rodenticidas/análise , Animais , Anticorpos Monoclonais/imunologia , Formação de Anticorpos , Anticoagulantes/sangue , Anticoagulantes/imunologia , Anticoagulantes/urina , Feminino , Limite de Detecção , Fígado/química , Camundongos Endogâmicos BALB C , Modelos Moleculares , Fenindiona/análise , Fenindiona/sangue , Fenindiona/imunologia , Fenindiona/urina , Rodenticidas/sangue , Rodenticidas/imunologia , Rodenticidas/urina , Suínos
19.
Inorg Chem ; 58(16): 10625-10628, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31390189

RESUMO

Carbenes are known as donor molecules to form with chloroalane adducts, which enhances their aerobic and thermal stabilities. In contrast, the insertion products (cAACH)AlCl2(cAAC) (1) and (cAACH)AlHCl(THF) (2; THF = tetrahydrofuran) have been formed in the reaction of a cyclic alkyl(amino)carbene (cAAC:) with HAlCl2, and H2AlCl, respectively. PhC(NtBu)2Li as the precursor for the reaction with HAlCl2 in a molar ratio of 2:1 can easily form compound [PhC(NtBu)2]2AlH (3) with five-coordinate aluminum. The new products have been studied by spectroscopic methods and single-crystal X-ray diffraction.

20.
BMC Cancer ; 19(1): 721, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31331297

RESUMO

BACKGROUND: The aim of this study was to assess the prognostic significance of preoperative systemic inflammation score (SIS) on patients with esophageal squamous cell carcinoma (ESCC). METHODS: A total of 357 ESCC patients who accepted radical esophagectomy between January 2008 and December 2009 at our institution were recruited in the analysis. The cut-off finder application was used to calculate the optimal cutoff values. The Chi-squared test or Fisher's exact test were used to analyze categorical variables. Overall survival (OS) was calculated using the Kaplan-Meier method and the log-rank test. Multivariate analysis was calculated using Cox regression analysis model. A model combining SIS was created and its performance was evaluated using the Akaike information criterion (AIC) and concordance index (C-index). RESULTS: The median follow-up time was 58 months (range, 1-84 months). The 5-year OS rate was 50% (95% CI, 49.94-50.06%). The optimal cut-off values for preoperative neutrophil to lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR) and serum albumin (Alb) were 2.27, 3.79 and 36.55, respectively. Univariate analyses revealed that gender (P = 0.047), T stage (P < 0.001), N stage (P < 0.001), vascular invasion (P < 0.001), tumor location (P = 0.018), tumor length(P < 0.001), NLR (P = 0.006), LMR (P = 0.007), serum Alb (P = 0.001), and SIS (P < 0.001) were significantly associated with OS. Independent prognostic factors for OS were T stage, N stage, tumor location, tumor length, and SIS. However, NLR was not an independent prognostic factor in multivariate analysis. The model combining SIS had smaller AIC and higher C-index compared to the model without SIS, which suggesting that the adding the SIS to the multivariate model increasing the predictive accuracy of the OS in the ESCC patients treated with radical esophagectomy and 3-field lymphadenectomy (3-FL). CONCLUSIONS: SIS may treat as a novel prognostic factor than NLR for ESCC patients who underwent radical esophagectomy and 3-FL. However, Larger-scale studies are needed to validate these findings.


Assuntos
Neoplasias Esofágicas/patologia , Neoplasias Esofágicas/cirurgia , Carcinoma de Células Escamosas do Esôfago/patologia , Carcinoma de Células Escamosas do Esôfago/cirurgia , Inflamação/patologia , Linfócitos/patologia , Neutrófilos/patologia , Índice de Gravidade de Doença , Adulto , Idoso , Idoso de 80 Anos ou mais , Distribuição de Qui-Quadrado , Confiabilidade dos Dados , Esofagectomia , Feminino , Seguimentos , Humanos , Estimativa de Kaplan-Meier , Excisão de Linfonodo , Masculino , Pessoa de Meia-Idade , Monócitos/patologia , Análise Multivariada , Estadiamento de Neoplasias , Período Pré-Operatório , Prognóstico , Curva ROC , Estudos Retrospectivos , Albumina Sérica Humana , Taxa de Sobrevida
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