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1.
Inorg Chem ; 58(16): 10625-10628, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31390189

RESUMO

Carbenes are known as donor molecules to form with chloroalane adducts, which enhances their aerobic and thermal stabilities. In contrast, the insertion products (cAACH)AlCl2(cAAC) (1) and (cAACH)AlHCl(THF) (2; THF = tetrahydrofuran) have been formed in the reaction of a cyclic alkyl(amino)carbene (cAAC:) with HAlCl2, and H2AlCl, respectively. PhC(NtBu)2Li as the precursor for the reaction with HAlCl2 in a molar ratio of 2:1 can easily form compound [PhC(NtBu)2]2AlH (3) with five-coordinate aluminum. The new products have been studied by spectroscopic methods and single-crystal X-ray diffraction.

2.
Anal Bioanal Chem ; 411(25): 6755-6765, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31388716

RESUMO

Diphacinone (DPN) is an extensively used anticoagulant rodenticide that is also considered a hazardous chemical, which poses a threat to nontarget species. DPN poisoning cases in humans or other species frequently occur, while rapid and sensitive detection methods are rarely reported. Thus, it is meaningful to develop an immunoassay for DPN detection with high sensitivity and specificity. In this study, a hapten was synthesized and then conjugated with carrier proteins to prepare the immunogens with different conjugation ratios for the preparation of antibody. After evaluation of the antisera using an indirect competitive enzyme-linked immunosorbent assay (icELISA) and statistical analysis, we found that the immunogen prepared using the N,N-dicyclohexylcarbodiimide (DCC) method with a conjugation ratio of 28.5 could elicit mice to generate antibodies with high performance. Using hybridoma technology, we obtained the specific monoclonal antibody (mAb) 4G5 with a half maximal inhibitory concentration (IC50) of 0.82 ng/mL in buffer solution. We initially explored the recognition mechanism of DPN/CLDPN and mAb from both conformational and electronic aspects. Then, mAb 4G5 was applied to develop icELISA for biological samples. The limits of detection (LODs) of icELISA were 0.28 µg/L, 0.32 µg/L, and 0.55 µg/kg for swine plasma, urine, and liver samples, respectively, and the recoveries ranged from 72.3 to 103.3% with a coefficient of variation (CV) of less than 12.3% in spiked samples. In summary, we developed a sensitive, specific, and accurate icELISA for the detection of DPN in biological samples, which showed potential in food safety analysis and clinical diagnosis. Graphical abstract.

3.
BMC Cancer ; 19(1): 721, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31331297

RESUMO

BACKGROUND: The aim of this study was to assess the prognostic significance of preoperative systemic inflammation score (SIS) on patients with esophageal squamous cell carcinoma (ESCC). METHODS: A total of 357 ESCC patients who accepted radical esophagectomy between January 2008 and December 2009 at our institution were recruited in the analysis. The cut-off finder application was used to calculate the optimal cutoff values. The Chi-squared test or Fisher's exact test were used to analyze categorical variables. Overall survival (OS) was calculated using the Kaplan-Meier method and the log-rank test. Multivariate analysis was calculated using Cox regression analysis model. A model combining SIS was created and its performance was evaluated using the Akaike information criterion (AIC) and concordance index (C-index). RESULTS: The median follow-up time was 58 months (range, 1-84 months). The 5-year OS rate was 50% (95% CI, 49.94-50.06%). The optimal cut-off values for preoperative neutrophil to lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR) and serum albumin (Alb) were 2.27, 3.79 and 36.55, respectively. Univariate analyses revealed that gender (P = 0.047), T stage (P < 0.001), N stage (P < 0.001), vascular invasion (P < 0.001), tumor location (P = 0.018), tumor length(P < 0.001), NLR (P = 0.006), LMR (P = 0.007), serum Alb (P = 0.001), and SIS (P < 0.001) were significantly associated with OS. Independent prognostic factors for OS were T stage, N stage, tumor location, tumor length, and SIS. However, NLR was not an independent prognostic factor in multivariate analysis. The model combining SIS had smaller AIC and higher C-index compared to the model without SIS, which suggesting that the adding the SIS to the multivariate model increasing the predictive accuracy of the OS in the ESCC patients treated with radical esophagectomy and 3-field lymphadenectomy (3-FL). CONCLUSIONS: SIS may treat as a novel prognostic factor than NLR for ESCC patients who underwent radical esophagectomy and 3-FL. However, Larger-scale studies are needed to validate these findings.

4.
J Agric Food Chem ; 67(31): 8573-8580, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31293156

RESUMO

Glycosylation endows both natural and synthetic small molecules with modulated physicochemical and biological properties. Plant and bacterial glycosyltransferases capable of decorating various privileged scaffolds have been extensively studied, but those from kingdom Fungi still remain underexploited. Here, we use a combination of genome mining and heterologous expression techniques to identify four novel glycosyltransferase-methyltransferase (GT-MT) functional modules from Hypocreales fungi. These GT-MT modules display decent substrate promiscuity and regiospecificity, methylglucosylating a panel of natural products such as flavonoids, stilbenoids, anthraquinones, and benzenediol lactones. Native GT-MT modules can be split up and regrouped into hybrid modules with similar or even improved efficacy as compared with native pairs. Methylglucosylation of kaempferol considerably improves its insecticidal activity against the larvae of oriental armyworm Mythimna separata (Walker). Our work provides a set of efficient biocatalysts for the combinatorial biosynthesis of small molecule glycosides that may have significant importance to the pharmaceutical, agricultural, and food industries.


Assuntos
Proteínas Fúngicas/química , Glicosiltransferases/química , Hypocreales/enzimologia , Metiltransferases/química , Fenóis/química , Animais , Biocatálise , Cristalografia por Raios X , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Glicosilação , Glicosiltransferases/genética , Glicosiltransferases/metabolismo , Hypocreales/genética , Inseticidas/química , Inseticidas/farmacologia , Metilação , Metiltransferases/genética , Metiltransferases/metabolismo , Mariposas/efeitos dos fármacos , Fenóis/farmacologia , Especificidade por Substrato
5.
BMC Oral Health ; 19(1): 90, 2019 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-31133012

RESUMO

BACKGROUND: The purpose of this study was to analyze the association between the genetic polymorphism of genes (PAX6, SOSTDC1and FAM20B) and the susceptibility to mesiodens. METHODS: This study was carried out on 50 patients with mesiodens and 50 controls. The family history of each patient with mesiodens were recorded. Genomic DNA was extracted from saliva samples, and single nucleotide polymorphisms were detected in all exons and exon/intron boundaries of PAX6, SOSTDC1 and FAM20B using Sanger sequencing. The data were analyzed using pearson chi-square test with theoretical frequency ≥ 5. For theoretical frequency less than 5 but at least 1 (≤20% cell), the data were analyzed by continuity correction. For the rest, Fisher's Exact test was used. A P-value< 0.05 was considered statistically significant. The Odds ratio (OR) and confidence intervals (CI) were recorded. RESULTS: Three polymorphisms were detected in PAX6. Two polymorphisms were detected in SOSTDC1. Twenty-nine polymorphisms were detected in FAM20B. Although, the T allele of FAM20B (rs3766626) appears to be associated with mesiodens (P = 0.051), there were no significant differences of PAX6/SOSTDC1/FAM20B gene polymorphisms between the two groups. The T allele of FAM20B (rs3766626) was associated with susceptibility to two mesiodens (P < 0.001; OR = 8.333; CI = 2.516-27.600). CONCLUSIONS: Lack of association between PAX6/SOSTDC1/FAM20B gene polymorphisms and mesiodens in the population studied was detected. Further studies with large samples on T allele of FAM20B (rs3766626) are needed.

6.
J Clin Oncol ; 37(20): 1695-1703, 2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-30920880

RESUMO

PURPOSE: This trial aimed to assess the efficacy and safety of the paclitaxel plus fluorouracil regimen versus the cisplatin plus fluorouracil regimen in definitive concurrent chemoradiotherapy (dCRT) in patients with locally advanced esophageal squamous cell carcinoma (ESCC). PATIENTS AND METHODS: Patients with locally advanced ESCC were enrolled and randomly assigned to either the paclitaxel plus fluorouracil group or the cisplatin plus fluorouracil group. The patients in the paclitaxel plus fluorouracil group were treated with paclitaxel and fluorouracil one cycle per week in dCRT for five cycles followed by paclitaxel and fluorouracil one cycle per month in consolidation chemotherapy for two cycles. The patients in the cisplatin/5-fluorouracil group were treated with cisplatin and fluorouracil one cycle per month in dCRT for two cycles followed by two cycles in consolidation chemotherapy. The radiotherapy dose was 61.2 Gy delivered in 34 fractions. The primary end point was 3-year overall survival (OS). RESULTS: Four hundred thirty-six patients with ESCC in six centers were recruited at a 1:1 ratio between April 2012 and July 2015. The median follow-up of the surviving patients was 48.7 months (interquartile range, 42.6-60.9). The 3-year OS was 55.4% in the paclitaxel plus fluorouracil group and 51.8% in the cisplatin plus fluorouracil group (hazard ratio, 0.905 [95% CI, 0.698 to 1.172]; P = .448). The 3-year progression-free survival was also not significantly different between the paclitaxel plus fluorouracil group and the cisplatin plus fluorouracil group (43.7% v 45.5%, respectively; hazard ratio, 0.973 [95% CI, 0.762 to 1.243]; P = .828). Compared with the cisplatin plus fluorouracil group, the paclitaxel plus fluorouracil group had significantly lower incidences of acute grade 3 or higher anemia, thrombocytopenia, anorexia, nausea, vomiting, and fatigue (P < .05), but higher incidences of acute grade 3 or higher leukopenia, radiation dermatitis, and radiation pneumonitis (P < .05). CONCLUSION: The paclitaxel plus fluorouracil regimen did not significantly prolong the OS compared with the standard cisplatin plus fluorouracil regimen in dCRT in patients with locally advanced ESCC.

7.
Anal Bioanal Chem ; 411(10): 2131-2140, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30719563

RESUMO

Herein, we developed a sensitive and quantitative flow assay for simultaneous detection of amantadine (AMD) and chloramphenicol (CAP) in chicken samples based on different CdSe/ZnS quantum dots (QDs). In contrast to other reports, the QDs could be excited by the same excitations that lowered the requirements for the matching instruments. Under the optimal conditions, the strategy permitted sensitive detection of AMD and CAP in a linear range of 0.23 to 1.02 ng/g and 0.02 to 0.66 ng/g. The limits of detection were 0.18 ng/g and 0.016 ng/g, respectively. Moreover, the whole detection process could be completed within 20 min with no additional sophisticated instruments and complicated operations. Spiked samples were analyzed using both QD-based lateral flow immunoassay (QD-LFIA) and commercial ELISA kits with good correlation (R2 = 0.96). Moreover, this study laid the foundation and simplified the development of the requisite instrument. Graphical abstract ᅟ.


Assuntos
Amantadina/análise , Anti-Infecciosos/análise , Cloranfenicol/análise , Análise de Alimentos/métodos , Imunoensaio/métodos , Carne/análise , Pontos Quânticos/química , Amantadina/isolamento & purificação , Animais , Anti-Infecciosos/isolamento & purificação , Compostos de Cádmio/química , Galinhas , Cloranfenicol/isolamento & purificação , Desenho de Equipamento , Análise de Alimentos/instrumentação , Imunoensaio/instrumentação , Limite de Detecção , Modelos Moleculares , Fitas Reagentes/análise , Compostos de Selênio/química , Sulfetos/química , Compostos de Zinco/química
8.
Mikrochim Acta ; 186(3): 193, 2019 02 18.
Artigo em Inglês | MEDLINE | ID: mdl-30778686

RESUMO

Pyrenylbutyric acid and streptavidin were coupled to films of reduced graphene oxide (rGO) and then conjugated to a biotinylated broad-spectrum monoclonal antibody against aflatoxins (AFs). It is shown that such films can efficiently and selectively capture AFs inculding AFB1, AFB2, AFG1, AFG2, AFM1 and AFM2. The rGO films were characterized by using scanning electron microscopy, energy-dispersive spectroscopy, and raman spectroscopy. The selectivity and purification performance of the antibody-loaded rGO films were investigated. They were applied to the purification of extremely small samples (100 µL) of AFs-spiked rabbit serum after enzymatic hydrolysis. The AFs were analyzed by ultra-performance liquid chromatography coupled to tandem mass spectrometry. The limits of detection for the six AFs investigated ranged from 50 to 170 pg·mL-1. The average recoveries of AFs in spiked rabbit serum samples ranged from 55% to 75%, with relative standard deviations of less than 9.4%. Graphical abstract Design of a multifunctional sandwich film that consists of a reduced graphene oxide film base, a pyrenylbutyric acid middle layer and a broad-specificity anti-AF monoclonal antibody surface layer. It was successfully applied to the determination of aflatoxins in only 100 µL of rabbit serum samples with satisfactory selectivity and acceptable accuracy.


Assuntos
Aflatoxinas/sangue , Anticorpos Monoclonais/química , Grafite/química , Animais , Técnicas Biossensoriais , Ácido Butírico/química , Cromatografia de Afinidade , Cromatografia Líquida de Alta Pressão , Oxirredução , Pirenos/química , Coelhos , Sensibilidade e Especificidade , Estreptavidina/química , Propriedades de Superfície , Espectrometria de Massas em Tandem
9.
Chem Commun (Camb) ; 55(16): 2360-2363, 2019 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-30724916

RESUMO

A silicon-aluminum heterocycle LAl(SiH2SiH2)2AlL (L = PhC(NtBu)2) (1) was prepared. Compound 1 exhibits a unique (N2Al)2(SiH2)4 centrosymmetric six-membered ring structure with a chair conformation, which is comparable with that of cyclohexane. Furthermore, two intermediate analogues, silylene-alane adduct LSi(AlMe3)-Si(AlMe3)L (2) and silylene-alane oxidative product [LAlHSiH2Mes]2 (3) were obtained. Compound 3 has an interesting arrangement of an Al-H and an SiH2 unit, which are in close vicinity to each other. 3 might be important to function as a catalyst, due to the already activated bridging Al-H bonds.

10.
Insect Biochem Mol Biol ; 105: 33-42, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30602123

RESUMO

Endoparasitoid wasps deliver a variety of maternal factors, such as venom proteins, viruses, and virus-like particles, from their venom and calyx fluid into hosts and thereby regulate the hosts' immune response, metabolism and development. The endoparasitoid, Microplitis mediator, is used as an important biological agent for controlling the devastating pest Helicoverpa armigera. In this study, using an integrated transcriptomic and proteomic analysis approach, we identified 75 putative venom proteins in M. mediator. The identified venom components were consistent with other known parasitoid wasps' venom proteins, including metalloproteases, serine protease inhibitors, and glycoside hydrolase family 18 enzymes. The metalloprotease and serpin family showed extensive gene duplications in venom apparatus. Isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative proteomics revealed 521 proteins that were differentially expressed at 6 h and 24 h post-parasitism, including 10 wasp venom proteins that were released into the host hemolymph. Further analysis indicated that 511 differentially expressed proteins (DEP) from the host are primarily involved in the immune response, material metabolism, and extracellular matrix receptor interaction. Taken together, our results on parasitoid wasp venoms have the potential to enhance the application of endoparasitoid wasps for controlling insect pest.


Assuntos
Interações Hospedeiro-Parasita , Proteínas de Insetos/metabolismo , Mariposas/parasitologia , Venenos de Vespas/metabolismo , Vespas/metabolismo , Animais , Feminino
11.
Front Immunol ; 9: 2301, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30405599

RESUMO

Parasitoid wasps depend on a variety of maternal virulence factors to ensure successful parasitism. Encapsulation response carried out by host hemocytes is one of the major host immune responses toward limiting endoparasitoid wasp offspring production. We found that VRF1, a metalloprotease homolog venom protein identified from the endoparasitoid wasp, Microplitis mediator, could modulate egg encapsulation in its host, the cotton bollworm, Helicoverpa armigera. Here, we show that the VRF1 proenzyme is cleaved after parasitism, and that the C-terminal fragment containing the catalytic domain enters host hemocytes 6 h post-parasitism. Furthermore, using yeast two-hybrid and pull-down assays, VRF1 is shown to interact with the H. armigera NF-κB factor, Dorsal. We also show that overexpressed of VRF1 in an H. armigera cell line cleaved Dorsal in vivo. Taken together, our results have revealed a novel mechanism by which a component of endoparasitoid wasp venom interferes with the Toll signaling pathway in the host hemocytes.

12.
Genome Biol Evol ; 2018 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-30496415

RESUMO

Haematococcus pluvialis is a freshwater species of Chlorophyta, family Haematococcaceae. It is well known for its capacity to synthesize high amounts of astaxanthin, which is a strong antioxidant that has been utilized in aquaculture and cosmetics. To improve astaxanthin yield and to establish genetic resources for H. pluvialis, we performed whole-genome sequencing, assembly, and annotation of this green microalga. A total of 83.1 Gigabases (Gb) of raw reads were sequenced. After filtering the raw reads, we subsequently generated a draft assembly with a genome size of 669.0 Mb, a scaffold N50 of 288.6 kb, and predicted 18,545 genes. We also established a robust phylogenetic tree from 14 representative algae species. With additional transcriptome data, we revealed some novel potential genes that are involved in the synthesis, accumulation, and regulation of astaxanthin production. In addition, we generated an isoform-level reference transcriptome set of 18,483 transcripts with high confidence. Alternative splicing analysis demonstrated that intron retention is the most frequent mode. In summary, we report the first draft genome of H. pluvialis. These genomic resources along with transcriptomic data provide a solid foundation for the discovery of the genetic basis for theoretical and commercial astaxanthin enrichment.

13.
BMJ Open ; 8(10): e020785, 2018 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-30344165

RESUMO

INTRODUCTION: Concurrent chemoradiation is the standard therapy for patients with local advanced oesophageal carcinoma unsuitable for surgery. Paclitaxel is an active agent against oesophageal cancer and it has been proved as a potent radiation sensitiser. There have been multiple studies evaluating paclitaxel-based chemoradiation in oesophageal cancer, of which the results are inspiring. However, which regimen, among cisplatin (TP), carboplatin (TC) or fluorouracil (TF) in combination with paclitaxel concurrent with radiotherapy, provides best prognosis with minimum adverse events is still unknown and very few studies focus on this field. The purpose of this study is to confirm the priority of TF to TP or TF to TC concurrent with radiotherapy in terms of overall survival and propose a feasible and effective plan for patients with local advanced oesophageal cancer. METHODS AND ANALYSIS: ESO-Shanghai 2 is a three-arm, multicenter, open-labelled, randomised phase III clinical trial. The study was initiated in July 2015 and the duration of inclusion is expected to be 4 years. The study compares two pairs of regimen: TF versus TP and TF versus TC concurrent with definitive radiotherapy for patients with oesophageal squamous cell carcinoma (OSCC). Patients with histologically confirmed OSCC (clinical stage II, III or IVa based on the sixth Union for International Cancer Control-tumour, node, metastasis classification) and without any prior treatment of chemotherapy, radiotherapy or surgery against oesophageal cancer will be eligible. A total of 321 patients will be randomised and allocated in a 1:1:1 ratio to the three treatment groups. Patients are stratified by lymph node status (N0, N1, M1a). The primary endpoint is overall survival and the secondary endpoint is progression-free survival and adverse events. ETHICS AND DISSEMINATION: This trial has been approved by the Fudan University Shanghai Cancer Centre Institutional Review Board. Trial results will be disseminated via peer reviewed scientific journals and conference presentations. TRIAL STATUS: The trial was initiated in July 2015 and is currently recruiting patients in all of the participating institutions above. TRIAL REGISTRATION NUMBER: NCT02459457.

14.
Arch Virol ; 163(12): 3357-3363, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30173353

RESUMO

Bracovirus is one of the two polydnavirus genera. Here, we used a cryo-EM analysis to reveal the near-native morphology of two nucleocapsid-containing model bracoviruses: Microplitis bicoloratus bracovirus (MbBV) and Microplitis mediator bracovirus (MmBV). MbBV and MmBV nucleocapsids have discernable cap structures in two distal regions with relatively high electron density. Adjacent to the end-cap structures are two electron-lucent rings. Some nucleocapsids were uniformly electron-dense and had a distinctive "helix-tail-like structure". Cryo-EM revealed inconsistent nucleocapsid diameters of 34-69.9 nm in MbBV and 46-69.9 nm in MmBV, and the largest observed cylindrical area length was expanded to 126 nm.


Assuntos
Nucleocapsídeo/ultraestrutura , Polydnaviridae/ultraestrutura , Vespas/virologia , Animais , Microscopia Crioeletrônica , Nucleocapsídeo/química , Nucleocapsídeo/isolamento & purificação , Polydnaviridae/química , Vírion/química , Vírion/isolamento & purificação , Vírion/ultraestrutura
15.
Rev Assoc Med Bras (1992) ; 64(2): 170-174, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29641678

RESUMO

OBJECTIVE: To investigate the diagnostic criteria of mediastinal lymph node metastasis (MLNM) in esophageal carcinoma (EC) by comparing the lymph node sizes measured by computed tomography (CT) and obtained by postoperative pathological examination. METHOD: A total of 305 EC patients were selected. MLNM location, shortest diameter and number were investigated one week before surgery, and then compared with their pathological findings. RESULTS: The receiver operating characteristic (ROC) curve analysis revealed that the minimum diameters of MLNM in the thoracic cavity was 8 mm (area under curve [AUC] = 0.766, Youden index = 0.424), 5 mm in supraclavicular fossa (AUC = 0.785, Youden index = 0.494), 6 mm in tracheoesophageal groove (AUC = 0.755, Youden index = 0.405); the sensitivity was increased significantly, and the Youden index was increased significantly when compared with 10 mm. CONCLUSION: The shortest diameter of diagnostic criteria of lymph nodes in EC could be less than 10 mm on CT.


Assuntos
Carcinoma/patologia , Neoplasias Esofágicas/patologia , Metástase Linfática/patologia , Neoplasias do Mediastino/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma/diagnóstico por imagem , Neoplasias Esofágicas/diagnóstico por imagem , Feminino , Humanos , Excisão de Linfonodo , Metástase Linfática/diagnóstico por imagem , Masculino , Neoplasias do Mediastino/secundário , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Tomografia Computadorizada por Raios X/métodos
16.
Radiat Oncol ; 13(1): 33, 2018 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-29482649

RESUMO

BACKGROUND: Concurrent chemoradiotherapy is a standard modality for locally advanced esophageal squamous cell carcinoma (ESCC) patients. Cisplatin combined with 5-fluorouracil continuous infusion (PF) remains the standard concurrent chemotherapy regimen. However, radiotherapy concurrent with PF showed a high incidence of severe side effects. Paclitaxel showed a promising radiosensitivity enhancement in the treatment of esophageal carcinoma in both vitro and vivo studies. The ESO-Shanghai 1 trial examines the hypothesis that paclitaxel plus 5-fluorouracil (TF) concurrent with radiotherapy has better overall survival and lower toxicity for patients with local advanced ESCC. METHOD: Four hundred thirty-six ESCC patients presenting with stage IIa to IVa will be enrolled in a prospective multicenter randomized phase 3 study. Patients will be randomized to either concurrent chemoradiotherapy with PF (cisplatin 25 mg/m2/d, d1-3, plus 5-fluorouracil 1800 mg/m2, continuous infusion for 72 h) once every 4 weeks for 2 cycles followed by consolidation chemotherapy for 2 cycles or concurrent chemoradiotherapy with weekly TF (5-fluorouracil 300 mg/m2, continuous infusion for 96 h plus paclitaxel 50 mg/m2, d1) for 5 weeks followed by consolidation chemotherapy (5-fluorouracil 1800 mg/m2, continuous infusion for 72 h, plus paclitaxel 175 mg/m2 d1) once every 4 weeks for 2 cycles. The radiotherapy dose is 61.2 Gy delivered in 34 fractions to the primary tumor including lymph nodes. The primary end-point is the 3-yr overall survival analyzed by intention to treat. The secondary endpoints are disease progression-free survival, local progression-free survival, and number and grade of participants with adverse events. DISCUSSION: The aim of this phase 3 study is to determine whether the TF regimen could replace the standard PF regimen for inoperable ESCC patients. An overall survival benefit of 12% at 3 years should be expected in the TF group to achieve this goal. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT01591135 . Registered 18 April 2012.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma de Células Escamosas/terapia , Quimiorradioterapia , Neoplasias Esofágicas/terapia , Projetos de Pesquisa , Adolescente , Adulto , Idoso , Cisplatino/administração & dosagem , Feminino , Fluoruracila/administração & dosagem , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Paclitaxel/administração & dosagem , Estudos Prospectivos , Taxa de Sobrevida , Resultado do Tratamento , Adulto Jovem
17.
Rev. Assoc. Med. Bras. (1992) ; 64(2): 170-174, Feb. 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-896438

RESUMO

Summary Objective: To investigate the diagnostic criteria of mediastinal lymph node metastasis (MLNM) in esophageal carcinoma (EC) by comparing the lymph node sizes measured by computed tomography (CT) and obtained by postoperative pathological examination. Method: A total of 305 EC patients were selected. MLNM location, shortest diameter and number were investigated one week before surgery, and then compared with their pathological findings. Results: The receiver operating characteristic (ROC) curve analysis revealed that the minimum diameters of MLNM in the thoracic cavity was 8 mm (area under curve [AUC] = 0.766, Youden index = 0.424), 5 mm in supraclavicular fossa (AUC = 0.785, Youden index = 0.494), 6 mm in tracheoesophageal groove (AUC = 0.755, Youden index = 0.405); the sensitivity was increased significantly, and the Youden index was increased significantly when compared with 10 mm. Conclusion: The shortest diameter of diagnostic criteria of lymph nodes in EC could be less than 10 mm on CT.

18.
Ann Clin Biochem ; 55(2): 254-263, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28490181

RESUMO

Purpose Following the analytical phase, the current practice of many hospital laboratories involves the manual verification of all test results followed by the production of the report. However, manual verification is a time-consuming and tedious process. In this paper, we provide a detailed description of how to design autoverification rules for thyroid function test profiles and sex hormones. Materials and methods We used DM2 (Data manager 2) to construct the algorithm and build the database for autoverification of thyroid function test profiles and sex hormones, with reference to Boolean logic, Auto 10-A and CLSI'88. The rules consist of checking quality control, instrument error flags, critical values, the analytical measurement range (AMR), the limit range, consistency check and delta check. Firstly, we established the rules in the DM2, collected clinical specimens for validation, then tested the rules in a 'live' environment. Results Agreement was achieved between manual verification by two senior laboratory personnel and verification using the autoverification rules in 99.78% of the cases. The total autoverification rate for all tests was 77.06%. Following implementation of the rules, the laboratory turnaround time (TAT) was reduced by 54.55% and staffing numbers fell from three to two whole time equivalents (WTE). Statistical analysis resulted in a kappa statistic of 0.99 ( P < 0.001). Moreover, after implementing the autoverification rules, the error rate fell to 0.04%, indicating that errors were almost completely eliminated. Conclusion Implementing autoverification rules can reduce TAT, minimize the number of samples that require manual verification and allow for a reduction in staffing numbers. It also allows laboratory staff to devote more time and effort to the handling of problematic test results and contributing to improved patient care.


Assuntos
Técnicas de Diagnóstico Endócrino/normas , Hormônios Esteroides Gonadais/sangue , Laboratórios Hospitalares/organização & administração , Laboratórios Hospitalares/normas , Validação de Programas de Computador , Testes de Função Tireóidea/normas , Algoritmos , Humanos , Controle de Qualidade , Valores de Referência
19.
J Cell Biochem ; 2017 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-29266356

RESUMO

Our study aims to explore the impact of microRNA-145 (miR-145) on proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells by modulating mTOR signaling pathway. Paired NSCLC and adjacent normal tissues were obtained from 50 NSCLC patients between January 2014 and January 2016. Human lung adenocarcinoma A549 cell line was assigned into the control, mimics, mimics-negative control (NC), inhibitor and inhibitor-NC groups. Quantitative RT-PCR and Western blot were applied to detect the expressions of miR-145 and mTOR. MTT, scratch test, Transwell and flow cytometry were carried out to analyze the effect of miR-145 on A549 cell proliferation, migration, invasion, apoptosis and cycle distribution and regulation of mTOR signaling pathway. MiR-145 expression in NSCLC tissues was lower than that in adjacent normal tissues, while mTOR expression was higher in NSCLC tissues than in adjacent normal tissues. Compared with the control and mimics groups, cell proliferation, migration and invasion abilities decreased in the mimics group; additionally, cell apoptosis rate increased and cells were mostly in G1 phase. MiR-145 inhibited the activation of mTOR signaling pathway. Compared with the control and inhibitor-NC groups, mTOR signaling pathway was activated and cell proliferation, migration and invasion abilities increased in the inhibitor group, and cell apoptosis rate decreased and cells were less in G1 phase. The study indicated that miR-145 expression was low in NSCLC and influenced NSCLC cells by negatively regulating mTOR signaling pathway. This article is protected by copyright. All rights reserved.

20.
Zootaxa ; 4329(3): 267-280, 2017 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-29242477

RESUMO

There is limited information about the ecology of freshwater flatworms in China, in particular, the members of the genus Macrostomum. Surveying freshwater bodies to explore the species diversity is the first step to obtain more ecological information about those flatworms. We hereby report the discovery of two new species of freshwater flatworms in Macrostomum. Macrostomum chongqingensis Lin and Wang, n. sp. was discovered from stones in the shallow water of Jialing River bed near Chongqing. Macrostomum zhaoqingensis Lin and Wang, n. sp. was discovered in the water of Xing Lake near Zhaoqing city, Guangdong province. Xing Lake is a part of an ancient river that has changed its course. For each species, we describe the morphology of the whole specimen, penis stylet, and sperm, as well as other internal anatomical features based on serial sections. We determined 18S, 28S rDNA and COI sequences from the two new species, and performed a phylogenetic analysis to confirm the status and evolutionary position of the two new species. We discuss the significance of our discoveries in terms of flatworm biodiversity studies in China and the Oriental realm.


Assuntos
Platelmintos , Animais , Evolução Biológica , China , DNA Ribossômico , Masculino , Filogenia
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