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1.
Anticancer Drugs ; 30(7): e0747, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31305293

RESUMO

Pyrvinium tosylate (PT) is an anthelminthic drug that has recently been shown to suppress various human cancers. However, whether PT is effective in nasopharyngeal carcinoma (NPC) has not been determined to date. In this work, we show the selective efficacy of PT in NPC while sparing normal nasopharyngeal epithelial cells, and its ability to increase chemosensitivity. We show that PT at 100 and 500 nmol/l significantly inhibits growth and induces apoptosis in several NPC cell lines without affecting normal nasopharyngeal epithelial cells. Using cell culture and xenograft mouse models, PT markedly enhances cisplatin's efficacy in NPC and the combination leads to almost complete tumor inhibition. Mechanism studies show that PT suppresses active, nuclear ß-catenin level and activity and increases Axin level in NPC cells. ß-Catenin overexpression completely reverses the inhibitory effects of PT, confirming that ß-catenin is the molecular mechanism of PT's action in NPC. In addition, the effects of PT on ß-catenin and Axin levels and on Wnt signaling in NPC cells are mediated by its activation of casine kinase 1α. Our work is the first to suggest that Wnt/ß-catenin is a selective target for NPC treatment, and provides the preclinical evidence on the translational potential of PT as a useful addition to the treatment armamentarium for NPC.

2.
Medicine (Baltimore) ; 98(30): e16588, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31348299

RESUMO

Pesticide exposure is a major health risk factor among agricultural workers, and poor protective behavior and a lack of awareness concerning the risks of pesticide use in developing countries may increase the intensity of pesticide exposure. This cross-sectional study aimed to explore the relationship between neurologic symptoms and protective behavior and awareness in relation to pesticide use in China. Latent class cluster analysis was used to categorize participants into 3 latent cluster subgroups, namely, a poor protective behavior subgroup, an excellent protective awareness and behavior subgroup, and a poor protective awareness subgroup, using a person-centered approach. Multivariate regression models were used to detect the association between the latent class cluster subgroups and self-reported neurologic symptoms. The results showed that poor protective behavior in pesticide use was an important negative predicator of neurologic symptoms such as reduced sleep quality, frequency of nightmares, debility, hypopsia, and hypomnesis. These findings suggest that targeted interventions for agricultural workers, especially local greenhouse farmers, are urgently needed to improve pesticide protection behavior.


Assuntos
Doenças do Sistema Nervoso/induzido quimicamente , Doenças do Sistema Nervoso/prevenção & controle , Exposição Ocupacional/prevenção & controle , Praguicidas/toxicidade , Roupa de Proteção , Adulto , Doenças dos Trabalhadores Agrícolas/induzido quimicamente , Doenças dos Trabalhadores Agrícolas/prevenção & controle , China , Estudos Transversais , Fazendeiros , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Análise de Classes Latentes , Masculino , Pessoa de Meia-Idade , Fatores Socioeconômicos
4.
Biomed Res Int ; 2019: 2518763, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31080814

RESUMO

Objective: The current study was aimed at using a latent profile analysis (LPA) model to classify greenhouse farmers into a potential cluster according to their exposure to pesticide residuals. Further, the association between self-rated health (SRH) and the cluster exposed to pesticide residual was explored. Methods: Four hundred sixty-four farmers from vegetable greenhouses were selected, their SRH information was gathered through questionnaires from the "Self-Rated Health Measurement Scale (SRHMS)" Version 1.0, and the corresponding pesticide residuals were detected in a laboratory. The linear mixed regression model was employed for association assessment. Results: Two latent clusters were extracted as samples, and the results showed that a high amount of pesticide residual accounted for poor physical health, but did not show statistical significance. In addition, an inverse significant association was observed between psychosocial symptoms and negative emotion and pesticide residual level. Furthermore, a diversity of significant relationship was observed in social health and its corresponding dimensions with latent cluster. Conclusions: LPA offers a holistic and parsimonious method to identify high-risk health clusters of greenhouse workers in various health aspects and allows for a personality-targeted intervention by a local health department.


Assuntos
Fazendeiros/psicologia , Exposição Ocupacional , Praguicidas/efeitos adversos , Verduras , Adulto , Agricultura , China , Estudos Transversais , Autoavaliação Diagnóstica , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Praguicidas/análise , Inquéritos e Questionários
5.
BMC Public Health ; 19(1): 373, 2019 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-30943937

RESUMO

BACKGROUND: In the northern region of China, many greenhouse vegetable farmers are exposed to high cumulative levels of pesticides due to long-term work in greenhouses that impacts their health. The aim of the current study was to identify the relationship between cumulative pesticide exposure and sleep disorders among farmers in Yinchuan, Northwest China. METHODS: A cross-sectional study was conducted for 3 consecutive years in 2015, 2016 and 2017. Using a random sampling to select the resident teams, 1366 participants were enrolled, and information was collected via face-to-face interviews by trained investigators. Ordinal logistic, multinomial logistic and poisson logistic regression models were used to identify the associations between cumulative exposure intensity (CEI) and sleep disorders. RESULTS: High CEI (OR = 1.56, 95% CI: 1.02-3.38) was associated with short sleep duration when compared with low CEI in the Full Model. CEI was not associated with long sleep duration. Self-rated sleep quality was associated with medium (OR = 1.46, 95% CI: 1.10-2.00) and high (OR = 2.50, 95% CI: 1.83-3.40) CEI. Similarly, having difficulty sleeping was associated with medium (OR = 1.52, 95% CI: 1.02-2.24) and high (OR = 1.74, 95% CI: 1.16-2.62) CEI. Differences in the associations by gender were also noted. CONCLUSION: CEI was associated with sleep disorders, and gender differences were observed. Efforts should be made by local governments to address sleep problems that result from cumulative pesticide exposure in farmers, and gender differences should be considered.


Assuntos
Agricultura , Fazendeiros , Exposição Ocupacional/efeitos adversos , Praguicidas/efeitos adversos , Transtornos do Sono-Vigília/etiologia , Sono/efeitos dos fármacos , Adulto , Idoso , China , Estudos Transversais , Fazendas , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Verduras , Adulto Jovem
6.
J Leukoc Biol ; 106(3): 733-747, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30861206

RESUMO

Host-pathogen interactions in tuberculosis (TB) should be studied at the disease sites because Mycobacterium tuberculosis (M.tb) is predominantly contained in local tissue lesions. T-cell immune responses are required to mount anti-mycobacterial immunity. However, T-cell immune responses modulated by programmed cell death protein 1 (PD-1) during tuberculosis pleurisy (TBP) remains poorly understood. We selected the pleural fluid mononuclear cells (PFMCs) from TBP and PBMCs from healthy donors (HD), and characterized PD-1-expresing T-cell phenotypes and functions. Here, we found that the PFMCs exhibited increases in numbers of PD-1-expressing CD4+ and CD8+ T cells, which preferentially displayed polarized effector memory phenotypes. The M.tb-specific Ag stimulation increased CD4+ PD-1+ and CD8+ PD-1+ T cells, which is in direct correlation with IFN-γ production and PD-L1+ APCs in PFMCs of these individuals. Moreover, blockage of PD-1/PD-L1 pathway enhanced the percentage of IFN-γ+ T cells, demonstrating that the PD-1/PD-L1 pathway played a negative regulation in T cell effector functions. Furthermore, CD4+ PD-1+ and CD8+ PD-1+ T-cell subsets showed greater memory phenotype, activation, and effector functions for producing Th1 cytokines than PD-1- counterparts. Thus, these PD-1+ T cells were not exhausted but appear to be central to maintaining Ag-specific effector. IL-12, a key immunoregulatory cytokine, enhanced the expression of PD-1 and restored a strong IFN-γ response through selectively inducing the phosphorylation of STAT4 in CD4+ PD-1+ T-bet+ and CD8+ PD-1+ T-bet+ T cells. This study therefore uncovered a previously unknown mechanism for T-cell immune responses regulated by PD-1, and may have implications for potential immune intervention in TBP.

7.
PLoS One ; 13(12): e0209566, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30576364

RESUMO

BACKGROUND: Self-rated health (SRH) has been shown to be a stronger comprehensive predictor of health status than the clinical record. Although an association between specific pesticide exposures and health conditions has been reported in different populations, data on the relationship between pesticides exposure intensity (PEI) and SRH in greenhouse farmers is scarce. The aim of the current study was to evaluate this association among vegetable greenhouse farmers in Yinchuan City, western China. METHODS: Three consecutive cross-sectional studies were conducted in the years 2015, 2016 and 2017. Face-to-face interviews by trained investigators, using questionnaires, were performed. PEI was calculated by a validated method and then categorized into high, middle and low groups. SRH was measured via a single ten-point scale question and then divided into excellent (score >5) and poor SRH (score ≤5). A multivariable logistic regression model was used to evaluate the association. Meanwhile, the dose-response and interaction effects were estimated. RESULTS: A steady association between high PEI and poor SRH (OR: 1.55, 95% CI: 1.05-2.28 in the full model) was identified. Although high PEI was significantly associated with poor SRH in males and the Han ethnicity group, no significant association was found with poor SRH in females or those of Hui ethnicity. Interaction effects of education level and frequency of breakfast with PEI were determined (Pinteraction = 0.04 and 0.02, respectively); synergistic enhanced effects for poor SRH were observed. CONCLUSION: These findings indicate that high PEI might be associated with poor SRH among vegetable greenhouse farmers. A lower education level and never eating breakfast contributed to an increased likelihood of poor SRH in those with high PEI. The local government should be making great efforts to promote healthy behaviors and improve protection awareness.


Assuntos
Fazendeiros , Nível de Saúde , Praguicidas/efeitos adversos , Autorrelato , Adulto , China , Autoavaliação Diagnóstica , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários , Verduras
8.
J Cell Mol Med ; 2018 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-30353641

RESUMO

Sustained adaptive immunity to pathogens provides effective protection against infections, and effector cells located at the site of infection ensure rapid response to the challenge. Both are essential for the success of vaccine development. To explore new vaccination approach against Mycobacterium tuberculosis (M.tb) infection, we have shown that Rv3615c, identified as ESX-1 substrate protein C of M.tb but not expressed in BCG, induced a dominant Th1-type response of CD4+ T cells from patients with tuberculosis pleurisy, which suggests a potential candidate for vaccine development. But subcutaneous immunization with Rv3615c induced modest T-cell responses systemically, and showed suboptimal protection against virulent M.tb challenge at the site of infection. Here, we use a mouse model to demonstrate that intranasal immunization with Rv3615c induces sustained capability of adaptive CD4+ T- and B-cell responses in lung parenchyma and airway. Rv3615c contains a dominant epitope of mouse CD4+ T cells, Rv3615c41-50 , and elicits CD4+ T-cell response with an effector-memory phenotype and multi-Th1-type cytokine coexpressions. Since T cells resident at mucosal tissue are potent at control of infection at early stage, our data show that intranasal immunization with Rv3615c promotes a sustained regional immunity to M.tb, and suggests a potency in control of M.tb infection. Our study warranties a further investigation of Rv3615c as a candidate for development of effective vaccination against M.tb infection.

9.
Comput Math Methods Med ; 2018: 7360685, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29853987

RESUMO

It is well known that the pollution and environmental fluctuations may seriously affect the outbreak of infectious diseases (e.g., measles). Therefore, understanding the association between the periodic outbreak of an infectious disease and noise and pollution still needs further development. Here we consider a stochastic susceptible-infective (SI) epidemic model in a polluted environment, which incorporates both environmental fluctuations as well as pollution. First, the existence of the global positive solution is discussed. Thereafter, the sufficient conditions for the nontrivial stochastic periodic solution and the boundary periodic solution of disease extinction are derived, respectively. Numerical simulation is also conducted in order to support the theoretical results. Our study shows that (i) large intensity noise may help the control of periodic outbreak of infectious disease; (ii) pollution may significantly affect the peak level of infective population and cause adverse health effects on the exposed population. These results can help increase the understanding of periodic outbreak patterns of infectious diseases.

10.
BMC Immunol ; 18(1): 38, 2017 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-28724400

RESUMO

BACKGROUND: Interleukin-9 (IL-9) was reported as an active participant in the pathogenesis of allergic asthma. This study aimed to investigate the major source ofIL-9 and its effect on interferon γ (IFN-γ) and immunoglobulin (Ig) secretion by B cells. METHODS: We isolated peripheral blood mononuclear cells from children with allergic asthma and healthy children. IL-9, IL-4 and IFN-γ expression were detected by ELISA, ELISpot and Flowcytometry. Expression of transcription factor PU.1 was measured by Western Blot. We evaluated the effect of IL-9 on B cell activation and Ig production. RESULTS: Results showed that compared with healthy children, levels of IL-9, IL-4 and PU.1 were elevated and levels of IFN-γ were lower in children with allergic asthma. IL-9-expressing CD4+ T cells did not co-express IL-4. Exogenous IL-9 inhibited IFN-γ production in a dose-dependent manner. Antigen-specific Th9 cells existed in children with house dust mite allergic asthma. IL-9 up-regulated expression of CD69 and CD25 on B cells and combination of IL-9 and IL-4 enhanced IgE production. CONCLUSIONS: In conclusion, our results showed that Th9 cells may be the major source of IL-9 in children with allergic asthma. In these patients, IL-9 impairs IFN-γ production and synergistically promotes IL-4-induced IgE secretion.


Assuntos
Asma/imunologia , Interleucina-9/imunologia , Leucócitos Mononucleares/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Adolescente , Animais , Asma/sangue , Células Cultivadas , Criança , Pré-Escolar , Relação Dose-Resposta Imunológica , Feminino , Humanos , Imunoglobulina E/imunologia , Imunoglobulina G/biossíntese , Interferon gama/imunologia , Interleucina-4/imunologia , Leucócitos Mononucleares/citologia , Ativação Linfocitária/imunologia , Masculino , Pyroglyphidae/imunologia , Subpopulações de Linfócitos T/citologia , Subpopulações de Linfócitos T/imunologia , Linfócitos T Auxiliares-Indutores/citologia
11.
Clin Sci (Lond) ; 131(15): 1859-1876, 2017 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-28588103

RESUMO

T-cell responses have been demonstrated to be essential for preventing Mycobacterium tuberculosis infection. The Th1-cytokines produced by T cells, such as INF-γ, IL-2, and TNF-α, not only limit the invasion of M. tuberculosis but also eliminate the pathogen at the site of infection. Bacillus Calmette-Guérin (BCG) is known to induce Th1-type responses but the protection is inadequate. Identification of immunogenic components, in addition to those expressed in BCG, and induction of a broad spectrum of Th1-type responses provide options for generating sufficient adaptive immunity. Here, we studied human pulmonary T-cell responses induced by the M. tuberculosis-specific antigen Rv3615c, a protein with a similar size and sequence homology to ESAT-6 and CFP-10, which induced dominant CD4+ T-cell responses in human tuberculosis (TB) models. We characterized T-cell responses including cytokine profiling, kinetics of activation, expansion, differentiation, TCR usage, and signaling of activation induced by Rv3615c compared with other M. tuberculosis-specific antigens. The expanded CD4+ T cells induced by Rv3615c predominately produced Th1, but less Th2 and Th17, cytokines and displayed effector/memory phenotypes (CD45RO+CD27-CD127-CCR7-). The magnitude of expansion and cytokine production was comparable to those induced by well-characterized the 6 kDa early secreted antigenic target (ESAT-6), the 10 kDa culture filtrate protein (CFP-10) and BCG. Rv3615c contained multiple epitopes Rv3615c1-15, Rv3615c6-20, Rv3615c66-80, Rv3615c71-85 and Rv3615c76-90 that activated CD4+ T cells. The Rv3615c-specific CD4+ T cells shared biased of T-cell receptor variable region of ß chain (TCR Vß) 1, 2, 4, 5.1, 7.1, 7.2 and/or 22 chains to promote their differentiation and proliferation respectively, by triggering a signaling cascade. Our data suggest that Rv3615c is a major target of Th1-type responses and can be a highly immunodominant antigen specific for M. tuberculosis infection.


Assuntos
Antígenos de Bactérias/imunologia , Proteínas de Bactérias/imunologia , Epitopos Imunodominantes/imunologia , Mycobacterium tuberculosis/imunologia , Células Th1/imunologia , Tuberculose Pleural/imunologia , Adulto , Idoso , Linfócitos T CD4-Positivos/imunologia , Estudos de Casos e Controles , Células Cultivadas , Citocinas/biossíntese , Feminino , Humanos , Memória Imunológica/imunologia , Imunofenotipagem , Ativação Linfocitária/imunologia , Masculino , Pessoa de Meia-Idade , Derrame Pleural/imunologia , Receptores de Antígenos de Linfócitos T alfa-beta/imunologia , Adulto Jovem
12.
Biol Trace Elem Res ; 169(2): 254-60, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26150404

RESUMO

Selenium is an important trace element for human health. Previous studies have raised concern that dietary selenium intake may change energy metabolism. AMP-activated protein kinase (AMPK) is a sensor of energy status that controls cellular energy homeostasis. We aimed to determine the effect of selenium on the phosphorylation of AMPK pathway between Se-deficient and normal Sprague-Dawley rats. Twenty-four weaning rats were fed either a Se-deficient diet (0.02 mg Se/kg) or a standard diet (0.18 mg Se/kg). After 109 days, total serum levels of non-esterified fatty acid and total amino acids were significantly higher and the serum insulin concentration was significantly lower in Se-deficient rats than in healthy controls. Selenium concentration and the activity of glutathione peroxidase (GPx) in myocardial tissue were significantly lower in Se-deficient rats. Importantly, mRNA levels of acetyl-CoA carboxylase beta (ACACB), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), and protein levels of p-AMPKα were increased in the Se-deficient group compared to normal controls (p < 0.05). In conclusion, our results suggest that selenium deficiency induces changes in metabolic and molecular parameters involved in energy metabolism in the AMPK pathway.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Metabolismo Energético , Selênio/deficiência , Proteínas Quinases Ativadas por AMP/genética , Aminoácidos/sangue , Animais , Ácidos Graxos não Esterificados/sangue , Feminino , Expressão Gênica/efeitos dos fármacos , Insulina/sangue , Masculino , Miocárdio/metabolismo , Fosforilação , Ratos Sprague-Dawley , Transdução de Sinais
13.
Huan Jing Ke Xue ; 36(1): 49-55, 2015 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-25898646

RESUMO

Dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO) are the most important biogenic organic dimethylated sulfur compounds in the ocean. The spatial distributions of these three sulfur compounds and their influencing factors were investigated in the East China Sea in June 2013. The mean concentrations of DMS, DMSPd, DMSPp, DMSOd and DMSOp in the surface seawater were 4.70, 7.00, 27.83, 13.66 and 10.78 nmol x L(-1), respectively. The horizontal distributions of DMS, DMSP and DMSO exhibited the similar patterns to that of chlorophyll a (Chl-a), with high values in coastal regions and low values in the open sea. DMS, DMSPd and DMSOp concentrations were significantly correlated with the levels of Chl-a, indicating that phytoplankton biomass might play an important role in controlling the concentrations of these sulfur compounds in the East China Sea. Moreover, positive relationships were observed between DMS and DMSPd and between DMSOd and DMS in the study area, which implied that the microbial degradation of DMSPd was the main source of DMS and DMSOd came mostly from the oxidation of DMS. The sea-to-air flux of DMS from the East China Sea in summer ranged from 0.62 to 33.98 micromol x (m2 x d)(-1), with an average of 9.71 micromol x (m2 x d)(-1).


Assuntos
Água do Mar/química , Sulfetos/análise , Compostos de Sulfônio/análise , Biomassa , China , Clorofila , Clorofila A , Oceanos e Mares , Fitoplâncton , Estações do Ano
14.
Artigo em Chinês | MEDLINE | ID: mdl-25195264

RESUMO

OBJECTIVE: To detect the levels and effects of protective bioactive substances such as NO, SOD and GSH in patients with obstructive sleep apnea syndrome (OSAHS) combining hypertension. METHOD: To collect the data from 99 snoring patients, which were divided into control group, OSAHS group, and OSAHS combined with hypertension group. The serum levels of NO), SOD and GSH in these patients were detected perioperatively, correlation analysis were carried out between these parameters. RESULT: The levels of NO, SOD and GSH in control group, OSAHS group and OSAHS combined with hypertension group reduced in turn with statistically significance, and had inverse correlations with apnea hyponea index (AHDI) and lowest saturation of blood oxygen. After operation, the levels of NO), SOD and GSH were increased and the blood pressure was decreased. CONCLUSION: The oxidative stress state is serious in patients with OSAHS. The oxidative stress process is more severe in patients with hypertension, which may play a role in the devolepment of hypertension in OSAHS patients.


Assuntos
Hipertensão/etiologia , Estresse Oxidativo , Apneia Obstrutiva do Sono/metabolismo , Adulto , Estudos de Casos e Controles , Glutationa/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Óxido Nítrico/sangue , Apneia Obstrutiva do Sono/complicações , Ronco , Superóxido Dismutase/sangue
15.
Acta Radiol ; 55(10): 1203-9, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24324278

RESUMO

BACKGROUND: Parotid tumor is an uncommon neoplasm. Only a few studies describing the sonographic features of pleomorphic adenomas and Warthin's tumors have been published, and most of those reports are based on a relatively small number of cases. PURPOSE: To define the sonographic characteristics of pleomorphic adenoma and Warthin's tumor in the parotid gland. MATERIAL AND METHODS: We retrospectively evaluated the sonographic features of 77 pleomorphic adenomas in 70 patients and 93 Warthin's tumors in 61 patients. The features included tumor size, shape, border, echogenicity, and homogeneity, the presence of a cystic area, acoustic enhancement, and the grade and distribution of vascularization. RESULTS: Lobulated lesions were observed in 49 pleomorphic adenomas and 36 Warthin's tumors. The lobulated shape was observed more frequently in pleomorphic adenomas than in Warthin's tumors (P = 0.005). Cystic areas within lesions were detected in 16 (16/77, 20.8%) pleomorphic adenomas and 42 (42/93, 45.2%) Warthin's tumors (P = 0.001). Of the pleomorphic adenomas, 77.9% had grade 0 or grade 1 vascularity, while 73.1% of Warthin's tumors had grade 2 or grade 3 vascularity (P = 0.000). Flow was peripheral in 53.2% of pleomorphic adenomas. The frequencies of central perfusion and mixed perfusion in Warthin's tumors were equal (47.3% vs. 47.3%, respectively). The differences in the vessel distribution between these two diseases was significant (P = 0.000). CONCLUSION: Ultrasound imaging characteristics, including multiple occurrences, shape, intrinsic cystic change, and the grade and distribution of tumor vessels, can be used to differentiate pleomorphic adenomas from Warthin's tumors.


Assuntos
Adenolinfoma/diagnóstico por imagem , Adenoma Pleomorfo/diagnóstico por imagem , Glândula Parótida/diagnóstico por imagem , Neoplasias Parotídeas/diagnóstico por imagem , Adulto , Idoso , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neovascularização Patológica/diagnóstico por imagem , Variações Dependentes do Observador , Estudos Retrospectivos , Ultrassonografia , Adulto Jovem
16.
Environ Sci Pollut Res Int ; 20(8): 5397-404, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23417438

RESUMO

A combined treatment of electrocoagulation and ultrasound was proposed to solve some problems which exist in the phosphorus removal processes in fine chemical industry. The intermittently discharged wastewater has the features of high initial phosphorus concentration and wide initial pH variation. The electrocoagulation-ultrasound effective performance for the removal of phosphorus was investigated. The results obtained from synthetic wastewater showed that the total phosphorus (TP) decreased from 86 to about 0.4 mg/L, and the removal efficiency reached about 99.6 %, when ultrasound was applied to the electrocoagulation cell under the optimum working conditions in 10 min. Comparatively, the TP removal efficiency of electrocoagulation group was 81.3 % and the ultrasound group has almost no change. Therefore, we can conclude that the electrocoagulation and ultrasound synergistic effect can effectively degrade high-phosphorus wastewater. We have discussed the impact of various parameters on the electrocoagulation-ultrasound based on the phosphorus removal efficiency. The results obtained from synthetic wastewater showed that the optimum working pH was found to be 6, allowing the effluent to be met the emission standards without pH adjustment. An increased current enhanced the speed of treatment significance, but higher current (>40 mA/cm(2)) enhanced ultrasonic cavitation effect causing flocculation ineffective. In addition, it was found that the optimum ultrasonic power was 4 W/cm(2) and the frequency was 20 kHz. The best ultrasound intervention and ultrasonic irradiation time were processed with electrocoagulation simultaneously. The results indicated that the electrocoagulation-ultrasound could be utilized as an attractive technique for removal of phosphate in the real wastewater.


Assuntos
Fósforo/química , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/química , Acústica , Técnicas Eletroquímicas , Concentração de Íons de Hidrogênio , Águas Residuárias
17.
Artigo em Chinês | MEDLINE | ID: mdl-23257094

RESUMO

OBJECTIVE: To study the teratogenicity of new high-energy compounds, 3, 4 two furazan-based oxidation furazan (DNTF) and the impact on human health, occupational exposure limits were provided for the following research. METHODS: Pregnant SD rats were randomly divided into five groups by Standard teratogenicity test, including three dose groups (5.0, 15.8, 50.0 mg/kg), the negative control (vegetable oil), and the positive control group (CP 10.0 mg/kg). Each 10 to 15 rats were in one group. Gavage was consecutive for rats during pregnancy 7 ∼ 12 d and then sacrifice after 20 d. RESULTS: There were no significantly difference between the three dose groups and negative controls in the pregnancy rate, the weight of pregnant rats, fetal weight, fetal growth, fetal malformation rate and internal organs, CONCLUSION: There were no maternal toxicity, embryo toxicity and teratogenicity for rats when DNTF in the range 5.0 ∼ 50.0 mg/kg.


Assuntos
Nitrofurazona/toxicidade , Teratogênios , Animais , Feminino , Gravidez , Ratos , Ratos Sprague-Dawley
18.
Clin Chem ; 54(1): 116-23, 2008 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17998268

RESUMO

BACKGROUND: Complement component 3 (C3) is an essential bridge linking innate immunity and adaptive immunity. We describe an immunonanogold catalytic resonance-scattering (RS) technique for assaying C3 in serum. METHODS: We used nanogold to label goat antihuman C3 antibody to obtain an immunonanogold RS probe for C3. The immune reaction between nanogold-labeled antibodies and antigens was carried out in Na(2)HPO(4)-sodium citrate buffer, pH 5.6, containing polyethylene glycol. After centrifuging the particle suspension, we used RS to monitor the catalytic effect of nanogold-labeled anti-C3 in the supernatant on the chlorauric acid-hydroxylamine (HAuCl(4)-NH(2)OH) particle reaction and used electron microscopy to monitor particle shape. We assayed 36 human serum samples with the immunonanogold catalytic RS assay and immunoturbidimetry. RESULTS: Nanogold-labeled anti-C3 had a marked catalytic effect on the reaction of HAuCl(4) and NH(2)OH to form particles, which exhibit a maximum RS peak at 585 nm. The decrease in RS intensity, DeltaI(RS), of the nanocatalytic system was proportional to C3 concentration from 5.0 to 160.0 ng/L. The detection limit for the C3 assay was 1.52 ng/L. Results obtained with serum samples agreed with those obtained with an immunoturbidimetric method. A linear regression analysis of 28 nonpathologic serum samples revealed a correlation coefficient of 0.960, with mean (SD) slope and intercept values of 0.787 (0.0218) g/L and 0.28 (0.026) g/L C3, respectively. CONCLUSION: The immunonanogold catalytic RS assay showed high sensitivity and good selectivity for measuring C3 in human serum. This method may become useful for diagnosing certain diseases, such as hepatitis.


Assuntos
Complemento C3/análise , Ouro , Nanopartículas Metálicas , Anticorpos/química , Catálise , Cloretos/química , Complemento C3/imunologia , Compostos de Ouro/química , Humanos , Hidroxilamina/química , Imunoensaio/métodos , Luz , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Nefelometria e Turbidimetria , Espalhamento de Radiação , Sensibilidade e Especificidade , Espectrofotometria
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