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1.
J Hazard Mater ; 442: 130012, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36182889

RESUMO

Nanoplastics (NPs) have become an emerging global environmental problem, and the toxicity of polystyrene nanoplastics (PS-NPs) in rice plants has received widespread attention. However, few studies have focused on silicon (Si)-mediated interactions between PS-NPs and rice. Thus, two forms of Si (organosilicon/inorganic silica) treated rice cells were exposure of positively or negatively charged NPs, PS-NH2 and PS-COOH, to evaluate the effects of Si for defense against PS-NPs toxicity in rice. The result showed PS-NH2 nanoparticles were accumulated at relatively low levels in cells compared with that of PS-COOH, but induced a higher accumulation of hydrogen peroxide (H2O2) and superoxide radicals (O2•-). However, both organosilicon and inorganic silica can generate more negative potential on the surfaces of cell wall to absorb large numbers of positively charged PS-NH2. In addition, they can prevent the uptake of both PS-NH2 and PS-COOH through reducing the porosity on the surface of the cell walls. These finally alleviated the toxicity of oxidative stress caused by PS-NPs and improved the viability of rice cells. Our findings demonstrated the significant contribution of Si in combating PS-NPs in rice.

2.
PeerJ ; 10: e14086, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36193422

RESUMO

Hepatic ischemia-reperfusion injury (HIRI) is one of the major sources of mortality and morbidity associated with hepatic surgery. Ac2-26, a short peptide of Annexin A1 protein, has been proved to have a protective effect against IRI. However, whether it exerts a protective effect on HIRI has not been reported. The HIRI mice model and the oxidative damage model of H2O2-induced AML12 cells were established to investigate whether Ac2-26 could alleviate HIRI by regulating the activation of IL-22/IL-22R1/STAT3 signaling. The protective effect of Ac2-26 was measured by various biochemical parameters related to liver function, apoptosis, inflammatory reaction, mitochondrial function and the expressions of IL-22, IL-22R1, p-STAT3Tyr705. We discovered that Ac2-26 reduced the Suzuki score and cell death rate, and increased the cell viability after HIRI. Moreover, we unraveled that Ac2-26 significantly decreased the number of apoptotic hepatocytes, and the expressions of cleaved-caspase-3 and Bax/Bcl-2 ratio. Furthermore, HIRI increased the contents of malondialdehyde (MDA), NADP+/NADPH ratio and reactive oxygen species (ROS), whereas Ac2-26 decreased them significantly. Additionally, Ac2-26 remarkably alleviated mitochondria dysfunction, which was represented by an increase in the adenosine triphosphate (ATP) content and mitochondrial membrane potential, a decrease in mitochondrial DNA (mtDNA) damage. Finally, we revealed that Ac2-26 pretreatment could significantly inhibit the activation of IL-22/IL22R1/STAT3 signaling. In conclusion, this work demonstrated that Ac2-26 ameliorated HIRI by reducing oxidative stress and inhibiting the mitochondrial apoptosis pathway, which might be closely related to the inhibition of the IL-22/IL22R1/STAT3 signaling pathway.

3.
Planta ; 256(4): 82, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36103054

RESUMO

MAIN CONCLUSION: Associated analysis of GWAS with RNA-seq had detected candidate genes responsible for cold stress and chilling acclimation in rice. Haplotypes of two candidate genes and geographic distribution were analyzed. To explore new candidate genes and genetic resources for cold tolerance improvement in rice, genome-wide association study (GWAS) mapping experiments with 351 rice core germplasms was performed for three traits (survival rate, shoot length and chlorophyll content) under three temperature conditions (normal temperature, cold stress and chilling acclimation), yielding a total of 134 QTLs, of which 54, 59 and 21 QTLs were responsible for normal temperature, cold stress and chilling acclimation conditions, respectively. Integrated analysis of significant SNPs in 134 QTLs further identified 116 QTLs for three temperature treatments, 53, 43 and 18 QTLs responsible for normal temperature, cold stress and chilling acclimation, respectively, and 2 QTLs were responsible for both cold stress and chilling acclimation. Matching differentially expressed genes from RNA-seq to 43 and 18 QTLs for cold stress and chilling acclimation, we identified 69 and 44 trait-associated candidate genes, respectively, to be classified into six and five groups, particularly involved in metabolisms, reactive oxygen species scavenging and hormone signaling. Interestingly, two candidate genes LOC_Os01g04814, encoding a vacuolar protein sorting-associating protein 4B, and LOC_Os01g48440, encoding glycosyltransferase family 43 protein, showed the highest expression levels under chilling acclimation. Haplotype analysis revealed that both genes had a distinctive differentiation with subpopulation. Haplotypes of both genes with more japonica accessions have higher latitude distribution and higher chilling tolerance than the chilling sensitive indica accessions. These findings reveal the new insight into the molecular mechanism and candidate genes for cold stress and chilling acclimation in rice.


Assuntos
Oryza , Aclimatação/genética , Resposta ao Choque Frio , Estudo de Associação Genômica Ampla , Oryza/genética , Oryza/metabolismo , Locos de Características Quantitativas/genética , RNA-Seq
4.
Talanta ; 253: 123906, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36122432

RESUMO

The Electrochemiluminescence (ECL)-based biosensors have received considerable attention in food contaminants and disease diagnosis, due to their fascinating advantages such as low cost, fast analysis speed, wide linear range, high sensitivity, and excellent anti-interference ability. Meanwhile, with the vigorous development and improvement of nanotechnology, biosensor assembly strategies tend to diversify and be multifunctional. This review focuses on the representative ECL biosensors in food safety and disease diagnosis reported by our research group and other research groups based on nanomaterials assembly strategies in recent years. According to the different roles of nanomaterials played in the constitution of ECL biosensors, nanomaterials would be divided into the following two categories to be summarized: (1) Nanomaterials for signal amplification. (2) Nanomaterials as ECL emitters. Finally, this review prospects the perspectives on the future development direction of ECL biosensor in food safety and disease diagnosis.

5.
Front Cell Infect Microbiol ; 12: 953027, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36061868

RESUMO

Quick differentiation of the circulating variants and the emerging recombinant variants of SARS-CoV-2 is essential to monitor their transmission. However, the widely used gene sequencing method is time-consuming and costly when facing the viral recombinant variants, because partial or whole genome sequencing is required. Allele-specific real time RT-PCR (qRT-PCR) represents a quick and cost-effective method in SNP genotyping and has been successfully applied for SARS-CoV-2 variant screening. In the present study, we developed a panel of 3 multiplex allele-specific qRT-PCR assays targeting 12 key differential mutations for quick differentiation of SARS-CoV-2 recombinant variants (XD and XE) and Omicron subvariants (BA.1 and BA.2). Two parallel multiplex qRT-PCR reactions were designed to separately target the protype allele and the mutated allele of the four mutations in each allele-specific qRT-PCR assay. The variation of Cp values (ΔCp) between the two multiplex qRT-PCR reactions was applied for mutation determination. The developed multiplex allele-specific qRT-PCR assays exhibited outstanding analytical sensitivities (with limits of detection [LoDs] of 2.97-27.43 copies per reaction), wide linear detection ranges (107-100 copies per reaction), good amplification efficiencies (82% to 95%), good reproducibility (Coefficient of Variations (CVs) < 5% in both intra-assay and inter-assay tests) and clinical performances (99.5%-100% consistency with Sanger sequencing). The developed multiplex allele-specific qRT-PCR assays in this study provide an alternative tool for quick differentiation of SARS-CoV-2 recombinant variants (XD and XE) and Omicron subvariants (BA.1 and BA.2).


Assuntos
COVID-19 , SARS-CoV-2 , Alelos , COVID-19/diagnóstico , Teste para COVID-19 , Humanos , RNA Viral/genética , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , SARS-CoV-2/genética
6.
J Int Med Res ; 50(9): 3000605221121964, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36071632

RESUMO

An extraintestinal fistula is a rare complication after appendectomy. To our knowledge, this study is the first to report a case of sigmoid abdominal wall fistula after appendectomy in a 45-year-old male patient who underwent appendectomy due to acute appendicitis. Purulent discharge was noted at the abdominal wall incision wound for more than 1 year postoperatively. The patient's clinical manifestation, medical history, physical examination, and auxiliary examination all suggested the formation of an abdominal sinus. After exploratory laparotomy at the Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, a diagnosis of sigmoid abdominal wall fistula was confirmed, and the fistula was cured after complete fistula resection. The reporting of this rare and atypical case may provide useful reference information for diagnosing and treating future cases of enterocutaneous fistula.


Assuntos
Parede Abdominal , Apendicite , Fístula Intestinal , Parede Abdominal/cirurgia , Apendicectomia/efeitos adversos , Apendicite/diagnóstico , Apendicite/cirurgia , Humanos , Fístula Intestinal/diagnóstico por imagem , Fístula Intestinal/etiologia , Laparotomia/efeitos adversos , Masculino , Pessoa de Meia-Idade
7.
BMC Plant Biol ; 22(1): 394, 2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-35945492

RESUMO

BACKGROUND: Carotenoid cleavage oxygenases (CCOs) include the carotenoid cleavage dioxygenase (CCD) and 9-cis-epoxycarotenoid (NCED), which can catalize carotenoid to form various apocarotenoids and their derivatives, has been found that play important role in the plant world. But little information of CCO gene family has been reported in litchi (Litchi chinensis Sonn.) till date. RESULTS: In this study, a total of 15 LcCCO genes in litchi were identified based on genome wide lever. Phylogeny analysis showed that LcCCO genes could be classified into six subfamilies (CCD1, CCD4, CCD7, CCD8, CCD-like, and NCED), which gene structure, domain and motifs exhibited similar distribution patterns in the same subfamilies. MiRNA target site prediction found that there were 32 miRNA target sites in 13 (86.7%) LcCCO genes. Cis-elements analysis showed that the largest groups of elements were light response related, following was plant hormones, stress and plant development related. Expression pattern analysis revealed that LcCCD4, LcNCED1, and LcNCED2 might be involving with peel coloration, LcCCDlike-b might be an important factor deciding fruit flavor, LcNCED2 and LcNCED3 might be related to flower control, LcNCED1 and LcNCED2 might function in fruitlet abscission, LcCCD4a1, LcCCD4a2, LcCCD1, LcCCD4, LcNCED1, and LcNCED2 might participate in postharvest storage of litchi. CONCLUSION: Herein, Genome-wide analysis of the LcCCO genes was conducted in litchi to investigate their structure features and potential functions. These valuable and expectable information of LcCCO genes supplying in this study will offer further more possibility to promote quality improvement and breeding of litchi and further function investigation of this gene family in plant.


Assuntos
Dioxigenases , Litchi , MicroRNAs , Carotenoides/metabolismo , Dioxigenases/genética , Frutas/genética , Frutas/metabolismo , Litchi/genética , Litchi/metabolismo , MicroRNAs/genética , Oxigenases/genética , Melhoramento Vegetal , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas/genética
8.
Molecules ; 27(13)2022 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-35807264

RESUMO

5-Fluorouracil (5-FU) is an effective anticancer drug widely used in the world. To improve therapy efficiency and reduce side effects, it is very important to frequently detect the concentration of 5-FU in blood samples of patients. In this work, a new type of lateral flow immunochromatographic assay (LFIA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive and specific detection of 5-FU in blood samples was developed. Au@Ag/Au nanoparticles (NPs) employing Au particles as the core and Ag/Au alloy as the shell were synthesized, characterized and used as the substrate in SERS-LFIA due to their high SERS enhancement and biocompatibility. The immunoprobe was made in the form of AuMBA@Ag/Au-Ab in which mercaptobenzoic acid (MBA, a common Raman active reporter) was embedded in the core-shell layer and the monoclonal antibody (mAb) against 5-FU was immobilized on the surface. The performance of SERS-LFIA was similar to that in colloidal gold based-LFIA, and the entire assay time was within 20 min. According to the color intensity on the testing (T) lines of LFIA strips visualized by eyes, the contents of 5-FU in the samples could be qualitatively or semi-quantitatively identified. Furthermore, by measuring the characteristic Raman intensities of MBA on T lines, quantitative detection of 5-FU in the samples were achieved. The IC50 and limit of detection (LOD) of the LFIA for 5-FU were found to be 20.9 pg mL-1 and 4.4 pg mL-1, respectively. There was no cross-reactivity (CR) of the LFIA with nine relative compounds, and the CR with cytosine, tegafur and carmofur were less than 4.5%. The recoveries of 5-FU from spiked blood samples were in the range of 78.6~86.4% with the relative standard deviation (RSD) of 2.69~4.42%. Five blood samples containing 5-FU collected from the Cancer Hospital were measured by SERS-LFIA, and the results were confirmed by LC-MS/MS. It was proven that the proposed method was able to simply and rapidly detect 5-FU in blood samples with high sensitivity, specificity, accuracy and precision.


Assuntos
Antineoplásicos , Nanopartículas Metálicas , Cromatografia Líquida , Fluoruracila , Ouro , Humanos , Imunoensaio/métodos , Nanopartículas Metálicas/química , Prata/química , Análise Espectral Raman/métodos , Espectrometria de Massas em Tandem
9.
Front Vet Sci ; 9: 902001, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35812889

RESUMO

The objective of the present study was to investigate the effects of N-carbamylglutamate (NCG) supplementation on metabolic profile and microbiota in ruminal content and feces of lactating dairy cows under heat stress (HS). Forty-eight lactating Holstein cows (154 ± 13.6 days in milk) were assigned randomly to four treatments (n = 12), to receive 0, 15, 20, or 25 g/day of commercial NCG (proportion: 97.7%) for the period of 60 days. The recorded ambient temperature-humidity index (THI) suggested that the cows were exposed to HS for almost the entire experimental period (average THI: 80.6). Samples of ruminal content and feces were collected at the end of the trial (day 60) to determine the biological effects of NCG supplementation on metabolome and microbiota using mass spectrometry-based metabolomics and 16S rRNA gene sequencing techniques, respectively. Results showed that NCG supplementation enhanced the levels of ruminal microbial protein, total volatile fatty acids (VFAs), and the molar proportion of propionate in the rumen, but lowered the ruminal pH, ammonia nitrogen (NH3-N), and the ratio of acetate to propionate. NCG at doses of 20 and 25 g/day reduced the community richness and diversity of ruminal microbiota with the decrease of Shannon and Simpson diversity. Compositions of ruminal and fecal microbiotas were altered by NCG, and the PICRUSt results revealed that metabolic pathways of the bacteria, such as amino acid metabolism, energy metabolism, and pyruvate metabolism, were enriched in NCG groups. Distinct changes in the metabolomic profile of ruminal fluid were observed between the control and NCG groups. Changes of 26 metabolites mainly involved in arginine metabolism, glutamate metabolism, and nitrogen metabolism were observed associated with NCG supplementation. These results provided new insights into the effects of NCG on metabolomic profile and microbiota in ruminal content and feces, and the optimal dose of NCG supplemented to dairy cows was 20 g/hd/day, which contributed to understanding the effects of NCG on HS in lactating dairy cows.

10.
Artigo em Inglês | MEDLINE | ID: mdl-35904570

RESUMO

OBJECTIVE: To evaluate the use of Janus kinase inhibitor (JAKi) in treating JDM and develop cytokine biomarkers of active disease. METHODS: This study involved a retrospective cohort study that evaluated JAKi in 101 JDM patients as well as a cross-sectional study of cytokines in 128 JDM patients and 30 controls between November 2017 and December 2021 in the Affiliated Children's Hospital of Capital Institute of Pediatrics (China). RESULTS: During the median follow-up period of 19 months, 65.5% of the patients improved rashes, and CAT-BM scores decreased. 39.6% of JDM patients eliminated glucocorticoids. Muscle strength was improved in all patients who had abnormal muscle strength before JAKi use. Patients and parents provided positive subjective reviews of JAKi, and no serious adverse events were reported. Potential side effects of JAKi included abnormal leukopenia (14/95) and cough (16/83), which affected over 10% of the JDM patients. In the cytokine analysis, 12/34 cytokines were significantly elevated in active JDM patients. Compared with active JDM patients with multiple phenotypes, active JDM patients with only rashes demonstrated lower cytokine levels. Anti-NXP2-positive active patients had lower cytokine levels compared with those without positive anti-NXP2 antibodies. Among all increased cytokines, IL-1RA changed most dramatically, reaching over 793 times the mean of normal values. We developed a panel composed of 6 cytokines to differentiate active or stable status in our cohort (AUC = 0.8486, P < 0.05). CONCLUSION: The preliminary evidence suggested that JAKi is a relatively safe and effective alternative for JDM patients. Cytokine profiles could well reflect the inflammatory status of JDM patients.

11.
Carbohydr Polym ; 291: 119601, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35698404

RESUMO

Membranes are the dominant material for seawater desalination and clean-water harvesting, which are commonly composed of synthetic polymers, showing low hydrophilicity and environmental hazard. Herein, we developed a low-cost, intrinsically green, superhigh-water flux Janus cellulose membrane (CEM) via a facile cellulase etching strategy. Coating cellulase on the single surface of cellulose membrane (such as top surface), triggers effective etching on its top section rather than bottom section, which architects an asymmetric-pore structure of the Janus CEM including porous top-and dense bottom-layer. Such distinction endows the Janus CEM with an unprecedented high-water flux of 135.75 LMH and a low salt-water ratio of 0.29 g·L-1 for 1 M NaCl solution, which is 17-time higher and 62-time lower than that of the pristine CEM. Our Janus CEM enables a promising participant for the advanced membrane materials toward versatile separation engineering.


Assuntos
Celulases , Purificação da Água , Celulose/química , Humanos , Membranas Artificiais , Osmose , Água/química
12.
Cell Biol Int ; 46(10): 1577-1587, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35702760

RESUMO

The current studies associated with tumor biology continue to describe a high correlation between tryptophan (Trp) metabolism and tumor progression. These findings reflect the complex underlying mechanism of tumor development and highlight the need to explore additional drug targets for carcinoma-associated diseases. In our study, we reported that elevated Trp metabolism was observed in highly malignant glioma tumor tissues from patients. The elevated Trp metabolism in glioma cells were induced by the overexpression of Trp 2,3-dioxygenase 2 (TDO2), which further contributed to the production of the metabolite kynurenine (Kyn). Subsequently, the Kyn derived from Trp metabolism was able to mediate the activation of the aryl hydrocarbon receptor (AhR) and downstream PI3K/AKT signals, resulting in the strengthening of tumor stemness and growth. Meanwhile, the activation of the AhR could promote the process of epithelial-mesenchymal transition in gliomas through a TGF-ß-dependent mechanism, leading to enhanced tumor invasion in vitro and in vivo. Inhibition of the AhR using StemRegenin 1 was demonstrated to suppress glioma growth and improve the outcome of traditional chemotherapy in subcutaneous tumor-bearing mice, representing a promising therapeutic target for clinical glioma treatment.


Assuntos
Dioxigenases , Glioma , Animais , Dioxigenases/metabolismo , Glioma/metabolismo , Cinurenina/metabolismo , Cinurenina/farmacologia , Camundongos , Fosfatidilinositol 3-Quinases/metabolismo , Receptores de Hidrocarboneto Arílico/metabolismo , Transdução de Sinais , Triptofano/metabolismo , Triptofano Oxigenase/metabolismo
13.
Talanta ; 249: 123655, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-35696979

RESUMO

5-Fluorouracil (5-FU) is an effective anticancer drug widely used in cancer treatment. In this study, two 5-FU derivatives containing a spacer arm with the carboxylic group at the end were synthesized, which were linked to the carrier proteins to form 5-FU-protein conjugates used as the immunogens for the production of monoclonal antibody (mAb). Based on the produced mAb, the highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for 5-FU detection was established. The IC50 and LOD values of the assay were found to be 19.5 ng mL-1 and 0.5 ng mL-1, respectively. There was no cross-reactivity (CR) of the ELISA with cytosine, thymine and uracil, which avoided the interference from inherent pyrimidines. The CR values of the assay with three substitutes of 5-FU (tegafur, 5-fluoro-2'-deoxyuridine, carmofur) were within 9.7%-17.6%. The produced mAb was also applied in sample extraction. The immuno-affinity column capable specific capturing 5-FU was prepared by immobilizing the mAb on Sepharose-4B gel and filling into a SPE column. The recoveries of 5-FU in spiked samples measured by ELISA were 72.4%-90.7% with RSD of 3.6%-8.3%. Five blood samples collected from patients were extracted by immuno-affinity column, then measured by ELISA and confirmed by HPLC-MS/MS. There was a good correlation between HPLC-MS/MS and ELISA. It is demonstrated that the developed ELISA combined with immuno-affinity extraction can be a powerful alternative method for the detection of 5-FU in blood samples.


Assuntos
Anticorpos Monoclonais , Antineoplásicos , Ensaio de Imunoadsorção Enzimática/métodos , Fluoruracila , Humanos , Espectrometria de Massas em Tandem/métodos
14.
Animals (Basel) ; 12(11)2022 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-35681930

RESUMO

The nutritional value of whole crop wheat hay (WCWH) harvested at different maturation stages are different, and its feeding effects on dairy cows have not been thoroughly evaluated. In this study, the in vitro digestibility of whole wheat (Nongda 22) hay harvested during the flowering, late milk and dough stages were evaluated using batch culture technique. The neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents of whole wheat hay decreased by 35.5% and 40.4%, respectively, whereas the non-fibrous carbohydrates (NFC) content increased by 50.3% in WCWH harvested during the dough stage as compared to the flowering stage (p < 0.01). The pH of the fermentation liquid and acetate to propionate ratio was greatest in the wheat harvested during the flowering stage and lowest during the dough stage (p = 0.03), whereas the volatile fatty acid (VFA) concentration was greatest during the dough stage and lowest during the flowering stage (p < 0.01). The dry matter loss (DML) was 9.6% and 6.2% greater (p < 0.01) during the late milk stage than in the flowering or dough stages, and the NDF loss (NDFL; p = 0.01) and ADF loss (ADFL; p < 0.01) was greater in both the flowering and late milk stages. In conclusion, though the content of NDF was lower in the dough stage, and the starch to NFC ratio was greater, we determined that the optimal harvest stage should be the late milk stage due to the greater dry matter digestibility, the relatively greater NFC content and the shorter planting days.

15.
Neuroscience ; 494: 152-166, 2022 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-35569643

RESUMO

The negative emotions caused by persistent pain, called affective pain, are known to seriously affect human physical and mental health. The anterior cingulate cortex (ACC), especially the rostral ACC (rACC) plays a key role in the development of this affective pain. N-methyl-d-aspartate (NMDA) receptors, which are widely distributed in the ACC, are involved in the regulation of emotional behavior. It is well known that activation of opioid receptors can relieve pain, but whether it can alleviate affective pain is not clear. In the present study, conditioned place avoidance (CPA) responses induced by complete Freund's adjuvant (CFA) were used to represent the affective pain of place aversion. The behavioral measurements were synchronously combined with multichannel electrophysiological recordings of the discharge frequency of rACC pyramidal neurons to explore whether affective pain could be alleviated by the synthetic opioid [D-Ala2, D-Leu5]-Enkefalin (DADLE), an agonist of δ-opioid receptors. To further investigate this treatment as a mechanism for the relief of affective pain in CFA-treated animals, we used whole-cell patch recordings in slice preparations of the rACC region to determine the dose-dependent effects of DADLE on NMDA receptor-mediated currents. Then, western blot was used to determine levels of phosphorylated NMDA receptor subunits GluN1, GluN2 and GluN3 as affected by the δ-opioid receptor activation. The results showed that activation of δ-opioid receptors down-regulates the phosphorylation of NMDA receptor subunits, thereby inhibiting NMDA currents, decreasing the discharge frequency of rACC pyramidal neurons, and reversing the CPA response. Thus, δ-opioid receptor activation in the rACC region can alleviate affective pain.


Assuntos
Giro do Cíngulo , Receptores de N-Metil-D-Aspartato , Receptores Opioides delta , Animais , Leucina Encefalina-2-Alanina , Adjuvante de Freund , Giro do Cíngulo/fisiologia , N-Metilaspartato , Dor/psicologia , Ratos , Ratos Sprague-Dawley , Receptores de N-Metil-D-Aspartato/metabolismo , Receptores Opioides delta/metabolismo
16.
Mikrochim Acta ; 189(6): 214, 2022 05 06.
Artigo em Inglês | MEDLINE | ID: mdl-35513500

RESUMO

A competitive electrochemiluminescence immunoassay was established based on the isoluminol-H2O2 (ABEI-H2O2) system catalyzed by cobalt hydroxide (Co(OH)2) to detect florfenicol residues in food. First , ultra-thin two-dimensional Co(OH)2 nanosheets were used as the catalyst of ABEI-H2O2 system, and excellent catalytic effects were acquired by catalytic decomposition of hydrogen peroxide with cobalt ions. Then, bimetal PdAg (Pd/Ag) alloy nanoparticles were used as a bridge to connect ABEI and antibody due to their good biocompatibility; Pd/Ag alloy nanoparticles also had a catalytic effect to further amplify the ECL signal in the system due to the synergistic catalytic effect of the bimetal. A competitive immunoassay strategy was used to detect florfenicol, where the florfenicol in the sample will compete with the antibody for the limited binding sites on the coating antigen. The ECL immunosensor for florfenicol detection shows high sensitivity, with a linear range from 10-4  to 102 ng mL-1, and a detection limit of 3.1 × 10-5 ng mL-1, where the scan potential was varied from 0 to 0.6 V vs Ag/AgCl . This work was the first to use Co(OH)2 nanosheets and bimetal PdAg catalytic signal amplification methods to design the sensor, which provides a novel, convenient and reliable strategy for ultra-sensitive detection of florfenicol, and other biological small molecules. A novel ECL immunosensor based on ABEI-H2O22.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Ligas , Técnicas Biossensoriais/métodos , Cobalto/química , Peróxido de Hidrogênio/química , Hidróxidos , Imunoensaio/métodos , Limite de Detecção , Medições Luminescentes/métodos , Luminol/análogos & derivados , Nanopartículas Metálicas/química , Tianfenicol/análogos & derivados
17.
Zhongguo Gu Shang ; 35(5): 442-7, 2022 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-35535532

RESUMO

OBJECTIVE: To observe clinical efficacy of chiropractic manipulation in the treatment of degenerative scoliosis (DS). METHODS: From June 2017 to September 2019, 120 patients with degenerative scoliosis were randomly divided into treatment group (60 cases) and control group(60 cases). The patients in treatment group were treated with chiropractic manipulation once every other day for 4 weeks. The patients in control group were treated with eperisone hydrochloride tablets combined with thoracolumbar orthopedic(TSLO)brace, oral eperisone hydrochloride tablets 50 mg three times a day, wearing TSLO brace for not less than 8 hours a day. The course of treatment was 4 weeks. After the patients were selected into the group, visual analogue scale (VAS) and Oswestry Disability Index (ODI) were recorded before treatment, 1, 2, 3, 4 weeks after treatment and 1 month after treatment. The full length X-ray of the spine was taken before and 4 weeks after treatment, and the scoliosis Cobb angle, sagittal vertical axis (SVA) and lumbar lordosis (LL) were measured and compared. The adverse reactions during the treatment were recorded. RESULTS: There were significant differences in VAS and ODI between two groups at each time point after treatment (P<0.001), VAS and ODI at 2 weeks after treatment (PVAS=0.025, PODI=0.032) and 3 weeks after treatment(PVAS=0.040, PODI=0.044) in treatment group were significantly different from those in control group, but there was no significant difference in VAS and ODI at other time points between treatment group and control group (P>0.05). There was significant difference in Cobb angle between treatment group(P=0.010) and control group(P=0.017) after treatment, but there was no significant difference in LL and SVA between treatment group and control group. There was no significant difference in Cobb angle, LL and SVA between two groups before and after treatment. During the treatment, there were 4 mild adverse reactions in the control group and no adverse reactions in the treatment group. CONCLUSION: Chiropractic manipulation can effectively relieve pain and improve lumbar function in patients with degenerative scoliosis. The onset of action is faster than that oral eperisone hydrochloride tablets combined with TSLO brace, and it has better safety and can improve Cobb angle of patients with degenerative scoliosis.


Assuntos
Lordose , Manipulação Quiroprática , Escoliose , Fusão Vertebral , Humanos , Vértebras Lombares , Estudos Retrospectivos , Escoliose/terapia , Resultado do Tratamento
18.
Environ Monit Assess ; 194(5): 343, 2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35389100

RESUMO

In this study, we propose and construct a novel model that measures regional green development level based on the "three-circle" conceptual framework for green development. Using Jiangsu Province in eastern China as a case study, the spatial-temporal characteristics and dynamics of the green development level from 2000 to 2020 were evaluated using a multi-source dataset at the grid-cell level. Our results show that (1) the analytical hierarchy process-based model proposed herein has higher reliability in terms of the development level measurement than principal component analysis and the entropy weight method. In addition, the average score of green development in the study area was approximately 0.53. Spatially, the green development level in the eastern coastal areas of the study area was found to be generally higher than in other regions, while that in southwestern regions is relatively low. In terms of sub-regions, the green development level scores of the study area have been ranked as follows: middle Jiangsu > southern Jiangsu > northern Jiangsu. (2) It was observed that the gravity center of the green development level can be divided into three stages during the study, with a whole had shifted to the north. (3) For most cities in Jiangsu, the green development level initially increased at first, then declined, and then increased again. (4) In the future, the green development level of Jiangsu Province should pay more attention to promoting regional coordinated development and relationships between society and the environment under rapid economic development.


Assuntos
Monitoramento Ambiental , Desenvolvimento Sustentável , China , Cidades , Desenvolvimento Econômico , Reprodutibilidade dos Testes
20.
Dis Markers ; 2022: 7550090, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35251376

RESUMO

OBJECTIVE: The aims of our experiment were to compare the microorganisms in meibomian gland secretions from patients with internal hordeolum before and after treatment using hypochlorous acid eyelid wipes, to elucidate the mechanism underlying hypochlorous acid eyelid wipe treatment of internal hordeolum. METHODS: This was a prospective, matched-pair study. A total of eight patients with internal hordeolum who attended the ophthalmology clinic of our hospital from April to August 2020 were included. Meibomian gland secretions were collected from subjects before treatment (Group A) and from patients cured after eyelid cleaning with hypochlorous acid eyelid wipes for 7 days (Group B). Samples were submitted to 16S rRNA high-throughput sequencing, and the resulting data were analyzed to compare the differences in the structure and composition of meibomian gland secretion microbial flora before and after treatment of internal hordeolum. RESULTS: A total of 2127 operational taxonomic units were obtained from the two groups of samples, and there was no significant difference in alpha diversity before and after eyelid cleaning. At the phylum level, there was no significant difference between the two groups. The predominant phyla in Group A included the following: Firmicutes (32.78% ± 20.16%), Proteobacteria (26.73% ± 7.49%), Acidobacteria (10.58% ± 11.45%), Bacteroidetes (9.05% ± 6.63%), Actinobacteria (8.48% ±1.77%), and Chloroflexi (3.15% ± 3.12%), while those in Group B were the following: Proteobacteria (31.86% ± 9.69%), Firmicutes (29.07% ± 24.20%), Acidobacteria (11.33% ± 7.53%), Actinobacteria (7.10% ± 1.98%), Bacteroidetes (5.39% ± 5.17%), and Chloroflexi (3.89% ± 3.67%). Starting from the class level, significant differences in microbial communities were detected before and after eyelid cleaning (P < 0.05). Linear discriminant analysis effect size analysis showed the core flora in Group A microbiome comprising Actinobacteria, Staphylococcus, Staphylococcaceae, Staphylococcus aureus, Ruminococcacea UCg-014, Ruminococcacea-UCG-014, Halomonadaceae, Neisseria, Methylobacterium, Frankiales, and Neisseria sicca, while those in Group B microbial were Streptococcus sp., Blautia, Bifidobacterium pseudocatenulatum, Subdoligranulum, Subdoligranulum variabile, Faecalibacterium, and Faecalibacterium prausnitzii. CONCLUSION: Eyelid cleaning with hypochlorous acid eyelid wipes does not change the biodiversity in the meibomian gland secretions of patients with internal hordeolum. Hypochlorous acid eyelid wipes may affect the internal hordeolum through broad-spectrum antibacterial action to effectively reduce the relative abundance of symbiotic pathogens, such as Staphylococcus, Neisseria, Actinomycetes, and Ruminococcus and increase that of Faecalibacterium prausnitzii and other symbiotic probiotics with anti-inflammatory effects.


Assuntos
Bactérias/genética , Terçol/tratamento farmacológico , Ácido Hipocloroso/uso terapêutico , Glândulas Tarsais/microbiologia , Microbiota , Oxidantes/uso terapêutico , Adulto , Biodiversidade , Feminino , Humanos , Masculino , Estudos Prospectivos , RNA Ribossômico 16S/genética
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