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1.
APL Bioeng ; 7(1): 016106, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36685676

RESUMO

Mechanical signals from extracellular matrix stiffness are important cues that regulate the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). However, the incorporation of BMSCs into soft hydrogels and the dominance of soft matrices for BMSC growth and differentiation limit the directed differentiation of BMSCs incorporated into hydrogels for tissue engineering, especially osteogenesis. Here, we found that the expression of miR-99b increased with increasing hydrogel stiffness and that miR-99b regulated the proliferation and differentiation of BMSCs seeded on the surface of substrates with different stiffnesses. Furthermore, miR-99b significantly promoted the migration of BMSCs in 3D hydrogels. Mechanistically, we demonstrated that matrix stiffness-sensitive miR-99b targets the mammalian target of the rapamycin signaling pathway to regulate the adipogenic and osteogenic differentiation of BMSCs. In addition, by modulating the expression of miR-99b, the osteogenic differentiation of BMSCs in soft 3D hydrogels was promoted. Consistently, the flexible BMSC-GelMA hydrogel transfected with miR-99b significantly promoted bone regeneration in the rat calvarial defect area. These results suggest that miR-99b plays a key role in the mechanotransduction and phenotypic transformation of BMSCs and may inspire new tissue engineering applications with MSCs as key components.

2.
G3 (Bethesda) ; 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36626328

RESUMO

Single-cell RNA-sequencing (scRNA-seq) offers unparalleled insight into the transcriptional programs of different cellular states by measuring the transcriptome of thousands of individual cells. An emerging problem in the analysis of scRNA-seq is the inference of transcriptional gene regulatory networks and a number of methods with different learning frameworks have been developed to address this problem. Here, we present an expanded benchmarking study of eleven recent network inference methods on six published scRNA-seq datasets in human, mouse, and yeast considering different types of gold standard networks and evaluation metrics. We evaluate methods based on their computing requirements as well as on their ability to recover the network structure. We find that, while most methods have a modest recovery of experimentally derived interactions based on global metrics such as Area Under the Precision Recall curve, methods are able to capture targets of regulators that are relevant to the system under study. Among the top performing methods that use only expression were SCENIC, PIDC, MERLIN or Correlation. Addition of prior biological knowledge and the estimation of transcription factor activities resulted in the best overall performance with the Inferelator and MERLIN methods that use prior knowledge outperforming methods that use expression alone. We found that imputation for network inference did not improve network inference accuracy and could be detrimental. Comparisons of inferred networks for comparable bulk conditions showed that the networks inferred from scRNA-seq datasets are often better or at par with the networks inferred from bulk datasets. Our analysis should be beneficial in selecting methods for network inference. At the same time, this highlights the need for improved methods and better gold standards for regulatory network inference from scRNAseq datasets.

3.
Technol Health Care ; 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36641698

RESUMO

BACKGROUND: Traditional Chinese medicine (TCM) has been widely recognized and accepted worldwide to provide favorable therapeutic effects for cancer patients. As Andrographis paniculata has an anti-tumor effect, it might inhibit lung cancer. OBJECTIVE: The drug targets and related pathways involved in the action of Andrographis paniculata against lung cancer were predicted using network pharmacology, and its mechanism was further explored at the molecular level. METHODS: This work selected the effective components and targets of Andrographis paniculata against the Traditional Chinese Medicine System Pharmacology (TCMSP) database. Targets related to lung cancer were searched for in the GEO database (accession number GSE136043). The volcanic and thermal maps of differential expression genes were produced using the software R. Then, the target genes were analyzed by GO and KEGG analysis using the software R. This also utilized the AutoDock tool to study the molecular docking of the active component structures downloaded from the PubChem database and the key target structures downloaded from the PDB database, and the docking results were visualized using the software PyMol. RESULTS: The results of molecular docking show that wogonin, Mono-O-methylwightin, Deoxycamptothecine, andrographidine F_qt, Quercetin tetramethyl (3',4',5,7) ether, 14-deoxyandrographolide, andrographolide-19-ß-D-glucoside_qt and 14-deoxy-11-oxo-andrographolide were potential active components, while AKT1, MAPK14, RELA and NCOA1 were key targets. CONCLUSION: This study showed the main candidate components, targets, and pathways involved in the action of Andrographis paniculata against lung cancer.

4.
Adv Med Educ Pract ; 14: 21-30, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36644766

RESUMO

Introduction: Nursing knowledge, critical thinking ability, and self-perceived confidence are imperative to nursing skills in professional nursing practice. Therefore, nurse educators are required to use teaching strategies that will help promote their knowledge, critical thinking, and self-confidence in complex contents such as the nursing of acute upper gastrointestinal bleeding (AUGIB). Purpose: This study compares the effect of student-led and instructor-led ward-round training methods on knowledge acquisition, critical thinking ability, and self-perceived confidence during AUGIB sessions. Methods: Forty nursing students in the first year of the Emergency Nursing Residency Program were randomly divided into a student-led ward round training group (SG) and an instructor-led ward round training group (IG) with a ratio of 1:1. A knowledge quiz, critical thinking ability test, and self-perceived confidence questionnaire were performed before and after the ward round training to assess both groups of students for their knowledge acquisition, critical thinking ability, and self-perceived confidence improvement. Feedback questionnaires were conducted after the training to evaluate students' perspectives and interests concerning the teaching module. Results: The scores of the post-training quiz were significantly higher than that of the pre-training quiz in both the SG (44.10±2.92 vs 31.10±4.27, p<0.001) and IG (32.35±2.21 vs 30.55±2.24, p=0.01). In the post-training quiz, scores achieved by the students from the SG (44.10±2.92) were significantly higher than those achieved by the students from the IG (32.35±2.21, p< 0.001). The level of self-perceived confidence improved significantly after ward round training in the SG (p< 0.001). However, there was no statistically significant difference in the IG with respect to the change from pre- to post-training (p=0.43).The students' critical thinking ability improved significantly in the SG (14.95±2.58 vs 7.10±1.79, p<0.001), while no significant improvement was found in the IG (7.91±2.28 vs 6.52±2.21, p=0.07) after ward round training. Conclusion: The teaching method of SWRT improves nursing students' knowledge acquisition, critical thinking ability, and self-perceived confidence in AUGIB.

5.
Huan Jing Ke Xue ; 44(1): 444-451, 2023 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-36635832

RESUMO

In order to explore the characteristics of organic carbon mineralization and the variation law of organic carbon components of an artificial forest in a loess hilly area, an artificial Robinia pseudoacacia forest restored for 13 years and the adjacent slope farmland were selected as the research objects, and indoor culture experiments under three different temperature treatments (15, 25, and 35℃) were carried out. The results indicated that the mineralization rate of soil organic carbon decreased sharply at first and then stabilized. The cumulative release of organic carbon increased rapidly in the initial stage of culture and gradually slowed in the later stage. Soil organic carbon mineralization in sloping farmland was more sensitive to temperature change, and its temperature sensitivity coefficient Q10 was 1.52, whereas that in R. pseudoacacia forest land was only 1.38. According to the fitting of the single reservoir first-order dynamic equation, the soil mineralization potential Cp of R. pseudoacacia forest land and slope farmland was between 2.02-4.32 g·kg-1 and 1.25-3.17 g·kg-1, respectively, that is, the mineralization potential of the R. pseudoacacia forest was higher. During the cultivation period, the content of various active organic carbon components decreased with time, and that in the R. pseudoacacia forest land was greater than that in the slope land. The cumulative carbon release of soil was significantly positively correlated with the contents of MBC and DOC (P<0.05), and Q10 (15-25℃) was negatively correlated with the contents of SOC, EOC, and SWC (P<0.05). These results could provide some reference for the study of soil carbon sequestration in loess hilly regions under climate change.


Assuntos
Robinia , Solo , Carbono/análise , Nitrogênio/análise , Florestas , Carvão Vegetal , China
6.
Cytotherapy ; 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36642683

RESUMO

Inflammation is the result of acute and chronic stresses, caused by emotional or physical trauma, or nutritional or environmental pollutants, and brings serious harm to human life and health. As an important cellular component of the innate immune barrier, the macrophage plays a key role in maintaining tissue homeostasis and promoting tissue repair by controlling infection and resolving inflammation. Several studies suggest that V Set and Ig domain-containing 4 is specifically expressed in tissue macrophages and is associated with a variety of inflammatory diseases. In this paper, we mainly summarize the recent research on V Set and Ig domain-containing 4 structures, functions, function and roles in acute and chronic inflammatory diseases, and provide a novel therapeutic avenue for the treatment of inflammatory diseases, including nervous system, urinary, respiratory and metabolic diseases.

7.
Commun Biol ; 6(1): 54, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36646841

RESUMO

The 22nd genetically encoded amino acid, pyrrolysine, plays a unique role in the key step in the growth of methanogens on mono-, di-, and tri-methylamines by activating the methyl group of these substrates for transfer to a corrinoid cofactor. Previous crystal structures of the Methanosarcina barkeri monomethylamine methyltransferase elucidated the structure of pyrrolysine and provide insight into its role in monomethylamine activation. Herein, we report the second structure of a pyrrolysine-containing protein, the M. barkeri trimethylamine methyltransferase MttB, and its structure bound to sulfite, a substrate analog of trimethylamine. We also report the structure of MttB in complex with its cognate corrinoid protein MttC, which specifically receives the methyl group from the pyrrolysine-activated trimethylamine substrate during methanogenesis. Together these structures provide key insights into the role of pyrrolysine in methyl group transfer from trimethylamine to the corrinoid cofactor in MttC.


Assuntos
Corrinoides , Metiltransferases , Metiltransferases/metabolismo , Metilaminas/metabolismo , Corrinoides/metabolismo
8.
Mol Ther ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36698311

RESUMO

Sepsis, a critical condition resulting from the systemic inflammatory response to severe microbial infection, represents a global public health challenge. However, effective treatment or intervention to prevent and combat sepsis is still lacking. Here, we reported that hyodeoxycholic acid (HDCA) has excellent anti-inflammatory properties in sepsis. We discovered that the plasma concentration of HDCA was remarkably lower in patients with sepsis and negatively correlated with the severity of the disease. Similar changes in HDCA levels in plasma and cecal contents samples were observed in a mouse model of sepsis, and these changes were associated with a reduced abundance of HDCA-producing strains. Interestingly, HDCA administration significantly decreased systemic inflammatory responses, prevented organ injury, and prolonged the survival of septic mice. We demonstrated that HDCA suppressed excessive activation of inflammatory macrophages via competitively blocking lipopolysaccharide (LPS) binding to toll-like receptor 4 (TLR4) and myeloid differentiation factor 2 (MD2) receptor complex, a unique mechanism that characterizes HDCA as an endogenous inhibitor of inflammatory signaling. Additionally, we verified these findings in TLR4 knockout mice. Our study highlights the potential value of HDCA as a therapeutic molecule for sepsis.

9.
Chemosphere ; 313: 137567, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36529168

RESUMO

Diflubenzuron (DFB) is a benzoylbenzourea insect growth regulator widely used in agriculture, horticulture, and vector control. Therefore, it can easily pollute water bodies and cause harm to aquatic life and ecosystems. To evaluate the impact of DFB on atyid shrimp Neocaridina palmate, the insecticide was applied, at 0, 0.74, 2.222, 6.667, 20, and 60 µg L-1, to indoor systems dominated by submerged plant Ceratophyllum demersum. The highest no observed effect concentration and the lowest observed effect concentration was determined to be 0.167 and 0.536 µg L-1, respectively, as it was counted with either activity or immune-reactive content of chitobiase. Subcellular indices were more sensitive, with a lowest observed effect concentration below 0.107 µg L-1. Principal response curves (PRC) and principal component analysis (PCA) showed that DFB reduced the biomass of C. demersum and the content of chlorophyll-a and phycocyanin in the media. The biomass of periphyton were promoted at the high concentrations. According to the PRC and PCA, DFB reduced the bacterial population related to photoautotrophy, sulphur reduction, and sulphur oxidation and it promoted those related to photoheterotrophy, nitrate reduction, nitrate denitrification, and nitrogen fixation. Besides, DFB reduced fungi related to denitrification. PRC and PCA showed that DFB had a negative impact on pH and dissolved oxygen levels and a positive impact on NH4-N, NO2-N, PO4-P, and conductivity, suggesting the deterioration in quality of water. This study provided useful information for understanding the ecotoxicological effects of DFB at population and community levels.


Assuntos
Decápodes , Diflubenzuron , Animais , Ecossistema , Nitratos , Água Doce/química , Água , Crustáceos
10.
Nat Genet ; 55(1): 144-153, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36581701

RESUMO

Networks are powerful tools to uncover functional roles of genes in phenotypic variation at a system-wide scale. Here, we constructed a maize network map that contains the genomic, transcriptomic, translatomic and proteomic networks across maize development. This map comprises over 2.8 million edges in more than 1,400 functional subnetworks, demonstrating an extensive network divergence of duplicated genes. We applied this map to identify factors regulating flowering time and identified 2,651 genes enriched in eight subnetworks. We validated the functions of 20 genes, including 18 with previously unknown connections to flowering time in maize. Furthermore, we uncovered a flowering pathway involving histone modification. The multi-omics integrative network map illustrates the principles of how molecular networks connect different types of genes and potential pathways to map a genome-wide functional landscape in maize, which should be applicable in a wide range of species.


Assuntos
Proteômica , Zea mays , Zea mays/genética , Genômica , Genes de Plantas
11.
Sci Total Environ ; 858(Pt 3): 160015, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36368379

RESUMO

Ecosystem service value (ESV) refers to the value of benefits provided by the ecosystem to people, and can reflect the quality of regional ecological environment. There have been few studies on ESV in arid regions experiencing dramatic land use changes. Also, many past ESV studies have obtained distorted results by using a simple linear function to examine the trade-offs between driving factors. This study quantified ESV in Xinjiang from 1990 to 2020 based on value equivalent method. Differences in ESV among ecosystem services in Xinjiang under different scenarios were simulated using a Bayesian network model. The results demonstrated land use changes in Xinjiang from 1990 to 2020, with construction land expanding the most significantly (dynamic index: 224.63 %), whereas grassland area decreased (dynamic index: -1.31 %) due to transformation to unused and cultivated land. ESV in Xinjiang presented an N-shaped variation trend from 1990 to 2020 and decreased by 309.6 × 108 CNY, with a variation rate of -20.35 %. The rank of the four categories of ecological services from 1990 to 2020 in terms of ESV was: regulating services > support services > cultural services > supply services. There was a gradual reduction in ESV in Xinjiang from 1990 to 2020. The rank of the different regions in terms of the reduction in ESV was: Northern Xinjiang (295.24 × 108 CNY) > Southern Xinjiang (280.94 × 108 CNY) > Eastern Xinjiang (109.76 × 108 CNY). Land use change was a direct driver of changes in ESV, whereas natural and social factors, such as precipitation, temperature, population, and policy factors, were indirect drivers. This study can act as a reference for sustainable management of ecosystem services in arid regions.


Assuntos
Ecossistema , Políticas , Humanos , Teorema de Bayes
12.
Glob Health Res Policy ; 7(1): 48, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36474302

RESUMO

BACKGROUND: Identifying factors associated with cardiovascular disease (CVD) is critical for its prevention, but this topic is scarcely investigated in Kashgar prefecture, Xinjiang, northwestern China. We thus explored the CVD epidemiology and identified prominent factors associated with CVD in this region. METHODS: A total of 1,887,710 adults at baseline (in 2017) of the Kashgar Prospective Cohort Study were included in the analysis. Sixteen candidate factors, including seven demographic factors, 4 lifestyle factors, and 5 clinical factors, were collected from a questionnaire and health examination records. CVD was defined according to International Clinical Diagnosis (ICD-10) codes. We first used logistic regression models to investigate the association between each of the candidate factors and CVD. Then, we employed 3 machine learning methods-Random Forest, Random Ferns, and Extreme Gradient Boosting-to rank and identify prominent factors associated with CVD. Stratification analyses by sex, ethnicity, education level, economic status, and residential setting were also performed to test the consistency of the ranking. RESULTS: The prevalence of CVD in Kashgar prefecture was 8.1%. All the 16 candidate factors were confirmed to be significantly associated with CVD (odds ratios ranged from 1.03 to 2.99, all p values < 0.05) in logistic regression models. Further machine learning-based analysis suggested that age, occupation, hypertension, exercise frequency, and dietary pattern were the five most prominent factors associated with CVD. The ranking of relative importance for prominent factors in stratification analyses showed that the factor importance generally followed the same pattern as that in the overall sample. CONCLUSIONS: CVD is a major public health concern in Kashgar prefecture. Age, occupation, hypertension, exercise frequency, and dietary pattern might be the prominent factors associated with CVD in this region.In the future, these factors should be given priority in preventing CVD in future.

13.
Front Pharmacol ; 13: 1031890, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36452229

RESUMO

Pulmonary fibrosis is a progressive and intractable lung disease with fibrotic features that affects alveoli elasticity, which leading to higher rates of hospitalization and mortality worldwide. Pulmonary fibrosis is initiated by repetitive localized micro-damages of the alveolar epithelium, which subsequently triggers aberrant epithelial-fibroblast communication and myofibroblasts production in the extracellular matrix, resulting in massive extracellular matrix accumulation and interstitial remodeling. The major cell types responsible for pulmonary fibrosis are myofibroblasts, alveolar epithelial cells, macrophages, and endothelial cells. Recent studies have demonstrated that metabolic reprogramming or dysregulation of these cells exerts their profibrotic role via affecting pathological mechanisms such as autophagy, apoptosis, aging, and inflammatory responses, which ultimately contributes to the development of pulmonary fibrosis. This review summarizes recent findings on metabolic reprogramming that occur in the aforementioned cells during pulmonary fibrosis, especially those associated with glucose, lipid, and amino acid metabolism, with the aim of identifying novel treatment targets for pulmonary fibrosis.

15.
Protein J ; 2022 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-36527585

RESUMO

Ginkgo seed is an important Chinese medicine and food resource in China, but the toxicity of ginkgo acid in it limits its application. Previous studies have found that salicylic acid decarboxylase (Sdc) has a decarboxylation degradation effect on ginkgo acid. In order to improve the decarboxylation ability of Sdc to Ginkgo acid, 11 residues of the Sdc around the substrate (salicylic acid) were determined as mutation targets according to the analysis of crystal structure of Sdc (PDB ID:6JQX), from Trichosporon moniliiforme WU-0401, and a total of 30 single point mutant enzymes and one compound mutant enzyme were obtained. With Ginkgo acid C15:1 as the substrate, it was found from activity assay that Sdc-Y64T and Sdc-P191A had higher decarboxylation activity, which increased by 105.18% and 116.74% compared with that of wild type Sdc, respectively. The optimal pH for Sdc Y64T and Sdc-P191A to decarboxylate Ginkgo acid C15:1 was 5.5, which is the same as the wild type Sdc. The optimal temperature of Sdc-P191A was 50 °C, which was consistent with that of the wild type Sdc, but the optimal temperature of the mutant Sdc-Y64T was 40 °C, which was 10 °C lower than that of wild type Sdc.

16.
Shock ; 2022 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-36533528

RESUMO

BACKGROUND: Our previous studies have shown that ameliorating mitochondrial damage in renal tubular epithelial cells (RTECs) can alleviate septic acute kidney injury (SAKI). It is reported that AMPK phosphorylation (p-AMPK) could ameliorate mitochondrial damage in renal tissue and Sirtuin 5 (SIRT5) over-expression significantly enhanced the level of p-AMPK in bovine preadipocytes. However, the role of SIRT5- mediated phosphorylation of AMPK in SAKI needs to be clarified. METHODS: WT/SIRT5 gene knockout mouse model of cecal ligation and puncture (CLP)-induced SAKI and a human kidney (HK)-2 cell model of LPS-induced SAKI were constructed. An AMPK chemical activator and SIRT5 overexpression plasmid were employed. Indexes of mitochondrial structure and function, level of p-AMPK, and expression of SIRT5 protein in renal tissue and RTECs were measured. RESULTS: After sepsis stimulation, the p-AMPK level was decreased, mitochondrial structure was disrupted, and ATP content was decreased. Notably, an AMPK activator alleviated SAKI. SIRT5 gene knockout significantly aggravated SAKI, while SIRT5 overexpression alleviated mitochondrial dysfunction following LPS stimulation, as manifested by the increase of p-AMPK level, the alleviation of mitochondrial structure damage, the restoration of ATP content, the decrease of pro-apoptotic protein expression, as well as the reduction of reactive oxygen species generation. CONCLUSION: Up-regulation of SIRT5 expression can attenuate mitochondrial dysfunction in RTECs and alleviate SAKI by enhancing the phosphorylation of AMPK.

17.
J Neuroinflammation ; 19(1): 308, 2022 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-36539796

RESUMO

BACKGROUND: Alcohol use disorders result from repeated binge and chronic alcohol consumption followed by negative effects, such as anxiety, upon cessation. This process is associated with the activation of NLRP3 inflammasome-mediated responses. However, whether and how inhibition of the NLRP3 inflammasome alters alcohol intake and anxiety behavior remains unclear. METHODS: A combination of drinking-in-the-dark and gavage was established in NLRP3-knockout and control mice. Behavior was assessed by open-field and elevated plus maze tests. Binge alcohol drinking was measured at 2 h and 4 h. A 2 h/4 h/24 h voluntary drinking was determined by a two-bottle choice paradigm. Western blotting and ELISA were applied to examine the levels of the NLRP3 inflammasome and- inflammatory factors, such as IL-1ß and TNF-α. Nissl staining was used to measure neuronal injury. The electrophysiological method was used to determine glutamatergic transmission in corticostriatal circuits. In vivo optogenetic LTP and LTD were applied to control the function of corticostriatal circuits on the behavior of mice. MCC950 was used to antagonize the NLRP3 inflammasome. RESULTS: The binge alcohol intake was decreased in NLRP3 KO mice compared to the control mice. During alcohol withdrawal, NLRP3 deficiency attenuated anxiety-like behavior and neuronal injury in the mPFC and striatum. Moreover, we discovered that glutamatergic transmission to striatal neurons was reduced in NLRP3 KO mice. Importantly, in vivo optogenetic induction of long-term potentiation (LTP) of corticostriatal circuits reversed the effects of NLRP3 deficiency on glutamatergic transmission and anxiety behavior. We also demonstrated that optogenetic induction of LTD decreased anxiety-like behavior and caused a reduction in glutamatergic transmission. Interestingly, NLRP3 deficiency or inhibition (MCC950 injection) attenuated the anxiety-like behavior, but it did not prevent DID + gavage paradigm-induced a persistent enhancement of drinking in a two-bottle choice at 2 and 4 days into withdrawal. CONCLUSION: Our results demonstrate that NLRP3 deficiency decreases binge alcohol intake and anxiety-like behavior through downregulation of glutamatergic transmission in corticostriatal circuits, which may provide an anti-inflammatory target for treating alcohol use disorders.


Assuntos
Alcoolismo , Síndrome de Abstinência a Substâncias , Camundongos , Animais , Inflamassomos , Proteína 3 que Contém Domínio de Pirina da Família NLR , Ansiedade , Consumo de Bebidas Alcoólicas , Camundongos Endogâmicos C57BL
18.
Front Oncol ; 12: 1058211, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36544699

RESUMO

Introduction: Adrenal myelolipomas are benign tumors composed mainly of lipomatous elements with myeloid cells. With the development of medical imaging technology, the detection rate has gradually increased. We report a case of adrenal myelolipoma successfully excised through the laparoscope and reviewed existing literature in recent ten years to summarize the feasibility of the laparoscopic approach for this tumor. Case presentation: Herein, we described a case of adrenal myelolipoma resected by laparoscope in a 63-year-old male patient. He did not have any other symptoms except the incidental finding of a left adrenal mass. An abdominal CT examination revealed a mixed-density lesion containing some amount of adipose tissue. In conjunction with the patient's willingness, we performed a laparoscopic operation to remove the lump. The definite diagnosis was confirmed as an adrenal myelolipoma according to the pathology. The patient recovered well postoperatively and without signs of recurrence at a 5-month follow-up. Conclusion: Adrenal myelolipoma is commonly benign, asymptomatic, and hormonal inactivity. A surgical strategy is suggested for high-complication-risk patients. The laparoscopic approach is safe and effective with an obvious advantage over open procedures.

19.
Opt Express ; 30(26): 46552-46559, 2022 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-36558606

RESUMO

Featuring with ultracompactness and subwavelength resolution, metasurface-assisted nanoprinting has been widely researched as an optical device for image display. It also provides a platform for information multiplexing, and a series of multiplexed works based on incident polarizations, operating wavelengths and observation angles have emerged. However, the angular-multiplexing nanoprinting is realized at the cost of image resolution reduction or the increase of fabrication difficulty, hindering its practical applications. Here, inspired by the Jacobi-Anger expansion, a phase-assisted design paradigm, called Bessel metasurface, was proposed for angular multiplexing nanoprinting. By elaborately designing the phase distribution of the Bessel metasurface, the target images can be encoded into the desired observation angles, reaching angular multiplexing. With the merits of ultracompactness and easy fabrication, we believe that our design strategy would be attractive in the real-world applications, including optical information storage, encryption/concealment, multifunctional switchable optical devices, and 3D stereoscopic displays, etc.

20.
Behav Sci (Basel) ; 12(12)2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36546958

RESUMO

Tourism experiences bring about physical or psychological feelings in tourists, which can not only leave tourists with deep memories, but also affect their behavioral intentions. Tourism experiences are meaningful only if they can be remembered and influence word of mouth and decision making. A better understanding of what influences tourism experience memory will help optimize the supply and further development of tourism destinations. This study explores the antecedents of rural tourism experience memory from the tourism supply perspective, revealing the mechanism of effect of these antecedents on tourists' tourism experience memory formation through a questionnaire-based survey of 556 participants in Xidi Village, China, and correlation and multiple regression analyses. The results show that perceptions about the supply of rural tourism destinations trigger positive emotions that, in turn, affect the formation of rural tourism experience memory. Through the mediating role of positive emotions, there is a significant correlation between perception of rural tourism destination supply and the formation of rural tourism experience memories.

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