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1.
Trauma Violence Abuse ; : 15248380211043825, 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34510982

RESUMO

BACKGROUND: This systematic review aims to estimate the extent to which childhood maltreatment influences self-compassion in later life. METHOD: Four English databases (Web of Science, PsycINFO, PubMed, and PsycARTICLES) and three Chinese databases (China National Knowledge Infrastructure, Wanfang, and Weipu) were systematically searched. We extracted data related to the associations between child maltreatment and self-compassion and pooled them using random effect models. FINDINGS: A total of 20 eligible studies were included involving 6,877 participants in the analyses. Overall child maltreatment was negatively related to self-compassion (r = -.28, p < .001); emotional abuse and neglect were negatively related to self-compassion (r = -.28, p < .01; r = -.31, p < .01, respectively) at a moderate level; and physical abuse, sexual abuse, and physical neglect were negatively related to self-compassion (r = -.12, p < .01; r = -.10, p < .01; and r = -.22, p < .001, respectively) at a small level. CONCLUSION: The results indicate that overall and subtypes of maltreatment are associated with decreased self-compassion, and child intervention programs focused on self-compassion should be designed to protect the well-being of individuals with a history of childhood maltreatment.

2.
Comput Intell Neurosci ; 2021: 1956394, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34539769

RESUMO

Aimed to address the low diagnostic accuracy caused by the similar data distribution of sensor partial faults, a sensor fault diagnosis method is proposed on the basis of α Grey Wolf Optimization Support Vector Machine (α-GWO-SVM) in this paper. Firstly, a fusion with Kernel Principal Component Analysis (KPCA) and time-domain parameters is performed to carry out the feature extraction and dimensionality reduction for fault data. Then, an improved Grey Wolf Optimization (GWO) algorithm is applied to enhance its global search capability while speeding up the convergence, for the purpose of further optimizing the parameters of SVM. Finally, the experimental results are obtained to suggest that the proposed method performs better in optimization than the other intelligent diagnosis algorithms based on SVM, which improves the accuracy of fault diagnosis effectively.


Assuntos
Algoritmos , Máquina de Vetores de Suporte , Análise de Componente Principal
3.
J Food Sci ; 86(10): 4466-4478, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34519051

RESUMO

During wine drinking, aroma release is mainly impacted by wine matrix compositions and oral physiological parameters. Notably, tannins in wine could interact with saliva protein to form aggregates which might also affect the volatility of volatiles. To explore tannins, saliva, and the interaction between them on the volatility of volatiles, the volatility of 16 aroma compounds in the model wine mixed with the commercial tannin extracts, human saliva, or both respectively, was evaluated in vitro static condition by using HS-SPME-GC/MS. The volatility of aroma compounds with high hydrophobicity or benzene ring appeared to decrease more when increasing the tannin levels. Specifically, the volatility of ethyl octanoate, ß-ionone, and guaiacol was decreased more than 20% by adding 2 g/L tannin extract. The addition of human saliva could significantly inhibit volatility of most aroma compounds in the model wine. Furthermore, the volatility of most aroma compounds in the mixture of tannins and human saliva was significantly lower than the control or the sample which were added with tannins or human saliva individually. The volatility of some aroma compounds in the mixture of the tannin and saliva was only around 50% or less, relative to the control. Two-way ANOVA analysis showed that there was a synergistic effect between tannin and saliva on decreasing the volatility of most aroma compounds (p < 0.05). Overall, understanding the effect of key factors such as tannins and saliva on volatility of volatiles could help to understand the sophisticated retronasal perceptions during wine tasting. PRACTICAL APPLICATION: The outputs of this research will be helpful in understanding the impact of tannins on retronasal aroma release during wine tasting. It might promote the control of tannins in the viticulture and brewing process to improve the retronasal perception of wine aroma.

4.
Comput Intell Neurosci ; 2021: 5468514, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34408778

RESUMO

Microcharge induction has recently been applied as a dust detection method. However, in complex environments, the detection device can be seriously polluted by noise. To improve the quality of the measured signal, the characteristics of both the signal and the noise should be analyzed so as to determine an effective noise removal method. Traditional removal methods mostly deal with specific noise signals, and it is difficult to consider the correlation of measured signals between adjacent time periods. To overcome this shortcoming, we describe a method in which wavelet decomposition is applied to the measured signal to obtain sub-band components in different frequency ranges. A time-lapse Pearson method is then used to analyze the correlation of the sub-band components and the noise signal. This allows the sub-band component of the measurement signal that has the strongest correlation with the noise to be determined. Based on multifractal detrended fluctuation analysis combined with empirical mode decomposition, the similarity between the signal sub-band components and the noise sub-band components is analyzed and three indices are employed to determine the multifractal characteristics of the sub-band components. The consistency between noise components and signal components is obtained and the main signal components are verified. Finally, the sub-band components are used to reconstruct the signal, giving the noise-free measured (microcharge induction) signal. The filtered signal presents smoother, multifractal features.


Assuntos
Poeira , Processamento de Sinais Assistido por Computador
5.
Artigo em Inglês | MEDLINE | ID: mdl-34426929

RESUMO

Aortic regurgitation (AR) is a volume overload disease causing eccentric left ventricular (LV) hypertrophy and eventually heart failure. There is currently no approved drug to treat patients with AR. Endoplasmic reticulum (ER) stress and ER stress-mediated apoptosis is involved in many cardiovascular diseases, but whether they also participate in AR-induced heart failure is still elusive. In this study, we found ER stress activation in myocardial samples from patients with AR. With a unique murine model of AR which induced eccentric cardiac hypertrophy and heart failure, we also found aggravation of cardiac ER stress and apoptosis, as evidenced by a reduction of Bcl-2/Bax ratio and an increase of caspase-3 cleavage. We then examined the signaling effectors involved in ER-initiated apoptosis and found volume overload specifically activated C/EBP homologous protein (CHOP), but not caspase-12 or Jun N-terminal kinase (JNK). Interestingly, tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor, improved cardiac function, and suppressed ER stress, apoptosis, and CHOP. Furthermore, genetic knockdown of CHOP inhibited cardiac Bcl-2/Bax ratio reduction and caspase-3 activation and rescued cardiac dysfunction. In summary, our findings suggest that ER stress-CHOP signaling is involved in the development of volume overload cardiac hypertrophy induced by AR through promoting cardiomyocytes apoptosis and provide a previously unrecognized target in heart failure induced by volume overload.

6.
Sci Total Environ ; 796: 148950, 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34271389

RESUMO

Boundary layer height (BLH) plays an important role in regulating global weather/climate, as well as the dispersion and transportation of pollutants. Until now, however, the attribution and contributions of different controlling factors to BLH long-term variability and trends have not been quantified on a global scale. The long-term radiosonde dataset was used in this study to retrieve global BLH climatology; seasonal, diurnal, long-term variation and trends were analyzed over a 39-year period (1980-2018). Statistical results show that the global distribution of the BLH and its trend have apparent day-night differences. BLH during daytime is deeper during clear-sky conditions compared to cloudy sky conditions, indicating a significant effect of clouds; BLH during nighttime is deeper under cloudy conditions. BLH was also found to vary over different land types; dry and hot soil exhibits a deeper BLH than those of wet and cool soil. The long-term variation and trend of BLH are highly influenced by near-surface meteorological parameters. In particular, based on multiple linear stepwise regression models and the contribution calculation method, this investigation initiatively quantifies the influences of meteorological parameters on global BLH long-term variation and trend. Our results emphasized that a 10 m wind speed (WS) and low tropospheric stability (LTS) have significant contributions to long-term BLH variation; WS and LTS anomalies alternately dominated the contribution of the diurnal cycle of the BLH anomaly. Annual BLH recorded an average increasing trend (38.9-42.1 m/decade), and LTS is more dominant than WS from a contribution perspective, especially for increased BLH anomaly. Contributions from near-surface temperature (T) and relative humidity (RH) also play important roles. However, a decreasing WS trend dominated the decreased trends of BLH anomaly, accounting for nearly 40% of the total contribution.


Assuntos
Poluentes Atmosféricos , Monitoramento Ambiental , Poluentes Atmosféricos/análise , China , Estações do Ano , Temperatura , Tempo (Meteorologia) , Vento
7.
J Interv Cardiol ; 2021: 9915759, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34220369

RESUMO

Background: Treatment of coronary intermediate lesions remains a controversy, and the role of arterial remodeling patterns determined by intravascular ultrasound in intermediate lesion is still not well known. The aim of this study was to investigate the impact of arterial remodeling of intermediate coronary lesions on long-term clinical outcomes. Methods: Arterial remodeling patterns were assessed in 212 deferred intermediate lesions from 162 patients after IVUS examination. Negative, intermediate, and positive remodeling was defined as a remodeling index of <0.88, 0.88∼1.0, and >1.0, respectively. The primary endpoint was the composite vessel-oriented clinical events, defined as the composition of target vessel-related cardiac death, target vessel-related myocardial infarction, and target vessel revascularization. Quantitative flow ratio was assessed for evaluating the functional significance of intermediate lesions. Results: 72 intermediate remodeling lesions were present in 66 patients, whereas 77 negative remodeling lesions were present in 71 patients, and 63 positive remodeling lesions were present in 55 patients. Negative remodeling lesions had the smallest minimum lumen area (4.16 ± 1.03 mm2 vs. 5.05 ± 1.39 mm2 vs. 4.85 ± 1.76 mm2; P < 0.01), smallest plaque burden (63.45 ± 6.13% vs. 66.12 ± 6.82% vs. 71.17 ± 6.45%; P < 0.01), and highest area stenosis rate (59.32% ± 10.15% vs. 54.61% ± 9.09% vs. 51.67% ± 12.96%; P < 0.01). No significant difference was found in terms of quantitative flow ratio among three groups. At 5 years follow-up, negative remodeling lesions had a higher rate of composite vessel-oriented clinical event (14.3%), compared to intermediate (1.4%, P=0.004) or positive remodeling lesions (4.8%, P=0.06). After adjusting for multiple covariates, negative remodeling remained an independent determinant for vessel-oriented clinical event (HR: 4.849, 95% CI 1.542-15.251, P=0.007). Conclusion: IVUS-derived negative remodeling is associated with adverse long-term clinical outcome in stable patients with intermediate coronary artery stenosis.


Assuntos
Doença da Artéria Coronariana/epidemiologia , Vasos Coronários/diagnóstico por imagem , Infarto do Miocárdio/epidemiologia , Revascularização Miocárdica/estatística & dados numéricos , Ultrassonografia de Intervenção , Remodelação Vascular , Idoso , Velocidade do Fluxo Sanguíneo , Doença da Artéria Coronariana/terapia , Estenose Coronária/diagnóstico por imagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Placa Aterosclerótica/diagnóstico por imagem , Estudos Retrospectivos
8.
Int J Cardiovasc Imaging ; 37(10): 2803-2813, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34059977

RESUMO

Discrepancy between coronary lesion severity and functional significance has always been a relevant issue in the management of patients undergoing coronary angiography and/or revascularization. We sought to investigate the relationship between quantitative flow ratio (QFR)-derived microcirculatory indices and anatomical-functional mismatch/reverse mismatch in intermediate coronary lesions. Intravascular ultrasound (IVUS) imaging and QFR were analyzed in 117 de novo intermediate coronary lesions. Lesions with QFR ≤ 0.8 were considered hemodynamically significant. Anatomical significance of the lesions was defined according to the best cutoff value of combined IVUS parameters for predicting QFR ≤ 0.8. QFR-derived microcirculatory indices including contrast-flow QFR minus fixed-flow QFR (cQFR-fQFR), hyperemic flow velocity and angiography-derived index of microcirculatory resistance (IMRangio) were calculated. The best cutoff values of IVUS parameters for predicting QFR ≤ 0.8 were minimum lumen area (MLA) 3.1mm2 and plaque burden (PB) 70%, with area under the curve of 0.635 and 0.703, respectively. The total discordance rate of lesion functional significance between IVUS and QFR assessments was 26.5%, with 21 lesions (17.9%) being classified as mismatch (MLA ≤ 3.1mm2 and PB ≥ 70% and QFR > 0.8) and 10 lesions (8.5%) as reverse-mismatch (MLA > 3.1 mm2 or PB < 70% and QFR ≤ 0.8). At multivariate analysis, IMRangio was identified as an independent predictor of mismatch (OR1.675, 95%CI:1.176-2.386, P = 0.004), whereas hyperemic flow velocity was identified as an independent predictor of reverse-mismatch (OR 1.233, 95%CI:1.073-1.416, P = 0.003). In intermediate coronary lesions, although MLA 3.1mm2 and PB 70% determined by IVUS are predictive of QFR-defined functional significance, the discordance rate remains substantial. QFR-derived microcirculatory indices are independently associated with anatomical-functional discordance between IVUS and QFR assessments.

9.
Neurol Sci ; 42(2): 467-473, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33409830

RESUMO

BACKGROUND AND PURPOSE: The COVID-19 pandemic has impacted the reperfusion therapy for acute ischemic stroke (AIS) patients. Huizhou City utilized its experience with the SARS and MERS breakouts to establish a reperfusion treatment program for AIS patients. METHOD: This is a retrospective study on 8 certified stroke hospitals in Huizhou City from January 2020 to May 2020. We analyzed the number of AIS patients with reperfusion therapy, stroke type (anterior/posterior circulation stroke), modes of transport to hospital, NIHSS score, onset to door time (ODT), door to needle time (DNT), and door to puncture time (DPT). The analysis was compared with baseline data from the same time period in 2019. RESULT: In 2020, the number of AIS patients receiving reperfusion therapy decreased (315 vs. 377), NIHSS score increased [8 (4, 15) vs. 7 [ (1, 2)], P = 0.024], ODT increased [126 (67.5, 210.0) vs. 120.0 (64.0, 179.0), P = 0.032], and DNT decreased [40 (32.5, 55) vs. 48 (36, 59), P = 0.003]. DPT did not change. Seventy percent of AIS patients indicated self-visit as their main mode of transport to the hospital. In both periods, mild stroke patients were more likely to self-visit than utilize emergency systems [2019: 152 (57.6%) vs. 20 (45.6%), P = 0.034; 2020: 123 (56.9%) vs. 5 (14.7%), P < 0.001]. The NIHSS score for self-visiting patients was lower for patients who utilized the ambulance system in both years [self-visit: 6.00 (3.00, 12.00), ambulance: 14.00 (9.00, 19.00), P < 0.001]. The volume of reperfusion patients was lower in 2020; however, the decrease was only significant (P = 0.028) in February 2020. CONCLUSION: During the COVID-19 pandemic in 2020, the number of AIS patients receiving reperfusion therapy significantly decreased when compared to the same period in 2019. The patients' condition increased severity, ODT increased, and the DNT decreased. DPT was not significant for self-visiting and ambulance patients. Moderate to severe stroke patients were more likely to utilize ambulance services.


Assuntos
COVID-19 , AVC Isquêmico/terapia , Avaliação de Processos em Cuidados de Saúde/estatística & dados numéricos , Reperfusão/estatística & dados numéricos , Tempo para o Tratamento/estatística & dados numéricos , Transporte de Pacientes/estatística & dados numéricos , Idoso , Idoso de 80 Anos ou mais , Ambulâncias/estatística & dados numéricos , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Índice de Gravidade de Doença
10.
Sci Total Environ ; 758: 143714, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33223157

RESUMO

Dust aerosol, one of the important light-absorbing impurities in snow and ice sheets in the Tibet Plateau (TP), can significantly affect the magnitude and timing of snow melting and glacier recession by altering the surface albedo. It is thus of great importance to understand the potential source and transport mechanism of the dust aerosol over the TP. A typical dust storm case, erupted from the Thar Desert (ThD) in South Asia on 1 to 4 May 2018, was selected to understand synoptic causes and a transport mechanism to the TP using the latest Second Modern-Era Retrospective analysis for Research and Applications (MERRA-2) reanalysis data. Comparing with active/passive satellite-based and AERONET-based observations, the MERRA-2 data provide both the spatio-temporal distribution and evolution process of the dust aerosol more accurately. This study also found that the entire Indian-Gangetic Plain (IGP), Southern India, the Bay of Bengal, and even the TP were influenced by the dust event. The synoptic analysis showed that the dust storm was caused jointly by an upper-level jet stream (ULJS), an upper trough and the subtropical high. A typical south-north secondary circulation adjacent its exit zone, mainly triggered by the ULJS, promoted much stronger and higher vertical uplift of the dust aerosols over the ThD. Consequently, those uplifted dust particles were easily transported to the TP across the majestic Himalayas by the southerly airflows in front of the low-pressure trough over Afghanistan and the southern branch trough over the Bengal Bay. These results indicate that dust aerosol and anthropogenic pollutions constrained and driven by the typical atmospheric circulation condition from South Asia are likely to be transported to the TP. Therefore, it is necessary to further pay attention to the influence of dust aerosols from South Asia on the weather and climate in the TP and its downstream areas.

11.
Cardiovasc Res ; 117(2): 585-599, 2021 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-32091582

RESUMO

AIMS: Endothelial cell (EC) homoeostasis plays an important role in normal physiological cardiac functions, and its dysfunction significantly influences pathological cardiac remodelling after myocardial infarction (MI). It has been shown that the sphingosine 1-phosphate receptor 1 (S1pr1) was highly expressed in ECs and played an important role in maintaining endothelial functions. We thus hypothesized that the endothelial S1pr1 might be involved in post-MI cardiac remodelling. METHODS AND RESULTS: Our study showed that the specific loss of endothelial S1pr1 exacerbated post-MI cardiac remodelling and worsened cardiac dysfunction. We found that the loss of endothelial S1pr1 significantly reduced Ly6clow macrophage accumulation, which is critical for the resolution of inflammation and cardiac healing following MI. The reduced reparative macrophages in post-MI myocardium contributed to the detrimental effects of endothelial S1pr1 deficiency on post-MI cardiac remodelling. Further investigations showed that the loss of endothelial S1pr1-reduced Ly6clow macrophage proliferation, while the pharmacological activation of S1pr1-enhanced Ly6clow macrophage proliferation, thereby ameliorated cardiac remodelling after MI. A mechanism study showed that S1P/S1pr1 activated the ERK signalling pathway and enhanced colony-stimulating factor 1 (CSF1) expression, which promoted Ly6clow macrophage proliferation in a cell-contact manner. The blockade of CSF1 signalling reversed the enhancing effect of S1pr1 activation on Ly6clow macrophage proliferation and worsened post-MI cardiac remodelling. CONCLUSION: This study reveals that cardiac microvascular endothelium promotes reparative macrophage proliferation in injured hearts via the S1P/S1PR1/ERK/CSF1 pathway and thus ameliorates post-MI adverse cardiac remodelling.

12.
Mol Plant Pathol ; 22(1): 108-116, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33146465

RESUMO

Fusarium oxysoporum f. sp. radicis-cucumerinum (Forc) is able to cause disease in cucumber, melon, and watermelon, while F. oxysporum f. sp. melonis (Fom) can only infect melon plants. Earlier research showed that mobile chromosomes in Forc and Fom determine the difference in host range between Forc and Fom. By closely comparing these pathogenicity chromosomes combined with RNA-sequencing data, we selected 11 candidate genes that we tested for involvement in the difference in host range between Forc and Fom. One of these candidates is a putative effector gene on the Fom pathogenicity chromosome that has nonidentical homologs on the Forc pathogenicity chromosome. Four independent Forc transformants with this gene from Fom showed strongly reduced or no pathogenicity towards cucumber, while retaining pathogenicity towards melon and watermelon. This suggests that the protein encoded by this gene is recognized by an immune receptor in cucumber plants. This is the first time that a single gene has been demonstrated to determine a difference in host specificity between formae speciales of F. oxysporum.

13.
Ann Clin Biochem ; 57(6): 435-443, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32985217

RESUMO

BACKGROUND: To date, China has no industry standard for reference intervals of paediatric blood biochemical markers. This study aimed to evaluate changes in biochemical markers in the venous blood of healthy children aged 29 days to 12 years, derived from the UniCel DxC 800 system, and establish appropriate reference intervals. METHODS: We analysed venous blood from 1980 healthy children for 20 biochemical markers. Reference intervals were established according to the Clinical and Laboratory Standards Institute C28-A3c guideline and compared with those of adults in China. RESULTS: All markers except for sodium and chlorine required partitioning by age, but not by sex. The reference intervals of total protein, albumin, globulin, carbon dioxide, urea nitrogen, creatinine and uric acid consistently increased with age in children, but were always lower than those of adults. Children aged 29 days to 12 years had a single combined RI for sodium and chloride, respectively; although the reference intervals in children were similar to those of adults, their upper limits were lower. The reference intervals of direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, potassium, calcium, magnesium, and phosphorus continued to decline with age. The reference intervals of total bilirubin, indirect bilirubin, and gamma-glutamyl transpeptidase initially declined followed by a slight rebound. CONCLUSIONS: While establishing reference intervals, most markers required partitioning by age (aged 29 days to 12 years); the partitioning scheme differed for each marker, and paediatric reference intervals differed from those for adults. It is therefore necessary to establish separate paediatric reference intervals.


Assuntos
Análise Química do Sangue , Biomarcadores/sangue , Criança , Pré-Escolar , China , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Valores de Referência
14.
Fungal Genet Biol ; 144: 103447, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32827756

RESUMO

Fortunately, no fungus can cause disease on all plant species, and although some plant-pathogenic fungi have quite a broad host range, most are highly limited in the range of plant species or even cultivars that they cause disease in. The mechanisms of host specificity have been extensively studied in many plant-pathogenic fungi, especially in fungal pathogens causing disease on economically important crops. Specifically, genes involved in host specificity have been identified during the last few decades. In this overview, we describe and discuss these host-specificity genes. These genes encode avirulence (Avr) proteins, proteinaceous host-specific toxins or secondary metabolites. We discuss the genomic context of these genes, their expression, polymorphism, horizontal transfer and involvement in pathogenesis.

15.
Biomed Res Int ; 2020: 3481623, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32626739

RESUMO

Evidences abound that HSF1 and ALDH2 are of cardioprotective effect, yet there is still no report on whether HSF1 can regulate ALDH2 to delay the occurrence of heart failure. We first established the pressure overload-induced heart failure model of mice by transverse aortic constriction (TAC) and discovered that, in the forming period of heart failure, changes of HSF1 and ALDH2 expression recorded the consistent trend. When HSF1 was upregulated/downregulated to delay/promote the occurrence of heart failure, PKC and ALDH2 also showed increased/decreased expression. And when ALDH2 was upregulated/downregulated, the role of HSF1 in delaying the occurrence of heart failure strengthened/weakened. Next, we used mechanical stretch to establish a pressure-stimulated myocardial hypertrophy model and discovered an increased expression of both HSF1 and ALDH2. When HSF1 was upregulated/downregulated to increase/decrease the expression of myocardial hypertrophy gene beta-MHC, PKC and ALDH2 recorded an increased/decreased expression. When an inhibitor was used to downregulate the expression of PKC in cardiomyocytes, we found that the role of HSF1 in upregulating ALDH2 beta-MHC weakened. These findings suggest that HSF1 can upregulate the expression of ALDH2 via PKC to promote pressure-stimulated myocardial compensatory hypertrophy, which is an important molecular pathway for HSF1 to ameliorate heart failure.


Assuntos
Aldeído-Desidrogenase Mitocondrial/metabolismo , Insuficiência Cardíaca/metabolismo , Fatores de Transcrição de Choque Térmico , Proteína Quinase C/metabolismo , Aldeído-Desidrogenase Mitocondrial/genética , Animais , Células Cultivadas , Regulação para Baixo/genética , Fatores de Transcrição de Choque Térmico/genética , Fatores de Transcrição de Choque Térmico/metabolismo , Masculino , Camundongos , Miocárdio/química , Miocárdio/metabolismo , Miocárdio/patologia , Miócitos Cardíacos/metabolismo , Proteína Quinase C/genética , Regulação para Cima/genética
16.
Bioorg Chem ; 101: 103870, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32512266

RESUMO

Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264). As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis. Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton. Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells. All the tested metabolites were found to have more potential anti-neuroinflammatory activity than the substrate. Especially, metabolites 2, 5 and 6 exhibited significant inhibition on NO production after hydroxylation at C-12 or C-15. Moreover, metabolite 2 dose-dependently reduced the LPS-induced protein expression of iNOS and COX-2.


Assuntos
Anti-Inflamatórios/farmacologia , Lipopolissacarídeos/farmacologia , Microglia/efeitos dos fármacos , Mucorales/metabolismo , Sistema Nervoso/efeitos dos fármacos , Óxido Nítrico/biossíntese , Compostos Orgânicos/farmacologia , Saponinas/farmacologia , Esteroides/farmacologia , Animais , Anti-Inflamatórios/química , Biotransformação , Catálise , Linhagem Celular , Ciclo-Oxigenase 2/metabolismo , Hidroxilação , Microglia/metabolismo , Estrutura Molecular , Sistema Nervoso/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Compostos Orgânicos/química , Saponinas/química , Análise Espectral/métodos , Esteroides/química
17.
Environ Microbiol ; 22(12): 4985-5004, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32452643

RESUMO

In Fusarium oxysporum f.sp. lycopersici, all effector genes reported so far - also called SIX genes - are located on a single accessory chromosome which is required for pathogenicity and can also be horizontally transferred to another strain. To narrow down the minimal region required for virulence, we selected partial pathogenicity chromosome deletion strains by fluorescence-assisted cell sorting of a strain in which the two arms of the pathogenicity chromosome were labelled with GFP and RFP respectively. By testing the virulence of these deletion mutants, we show that the complete long arm and part of the short arm of the pathogenicity chromosome are not required for virulence. In addition, we demonstrate that smaller versions of the pathogenicity chromosome can also be transferred to a non-pathogenic strain and they are sufficient to turn the non-pathogen into a pathogen. Surprisingly, originally non-pathogenic strains that had received a smaller version of the pathogenicity chromosome were much more aggressive than recipients with a complete pathogenicity chromosome. Whole genome sequencing analysis revealed that partial deletions of the pathogenicity chromosome occurred mainly close to repeats, and that spontaneous duplication of sequences in accessory regions is frequent both in chromosome deletion strains and in horizontal transfer strains.


Assuntos
Cromossomos Fúngicos/genética , Fusarium/genética , Fusarium/patogenicidade , Deleção Cromossômica , Proteínas de Fluorescência Verde/genética , Proteínas Luminescentes/genética , Doenças das Plantas/genética , Fatores de Transcrição/genética , Virulência/genética
18.
Mol Plant Pathol ; 21(6): 761-776, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32246740

RESUMO

Fusarium oxysporum f. sp. radicis-cucumerinum (Forc) causes severe root rot and wilt in several cucurbit species, including cucumber, melon, and watermelon. Previously, a pathogenicity chromosome, chrRC , was identified in Forc. Strains that were previously nonpathogenic could infect multiple cucurbit species after obtaining this chromosome via horizontal chromosome transfer (HCT). In contrast, F. oxysporum f. sp. melonis (Fom) can only cause disease on melon plants, even though Fom contains contigs that are largely syntenic with chrRC . The aim of this study was to identify the genetic basis underlying the difference in host range between Fom and Forc. First, colonization of different cucurbit species between Forc and Fom strains showed that although Fom did not reach the upper part of cucumber or watermelon plants, it did enter the root xylem. Second, to select candidate genomic regions associated with differences in host range, high-quality genome assemblies of Fom001, Fom005, and Forc016 were compared. One of the Fom contigs that is largely syntenic and highly similar in sequence to chrRC contains the effector gene SIX6. After HCT of the SIX6-containing chromosome from Fom strains to a nonpathogenic strain, the recipient (HCT) strains caused disease on melon plants, but not on cucumber or watermelon plants. These results provide strong evidence that the differences in host range between Fom and Forc are caused by differences between transferred chromosomes of Fom and chrRC , thus narrowing down the search for genes allowing or preventing infection of cucumber and watermelon to genes located on these chromosomes.


Assuntos
Cromossomos Fúngicos/genética , Cucumis sativus/microbiologia , Cucurbitaceae/microbiologia , Fusarium/genética , Especificidade de Hospedeiro , Doenças das Plantas/microbiologia , Fusarium/patogenicidade , Fusarium/fisiologia , Virulência
19.
Catheter Cardiovasc Interv ; 95 Suppl 1: 598-605, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31957972

RESUMO

BACKGROUND: Dual antiplatelet therapy (DAPT) with aspirin and a P2Y12 receptor inhibitor has become the standard of care to reduce thrombotic events in patients with acute coronary syndrome or after percutaneous coronary intervention (PCI). The role of routine platelet function testing (PFT) in patients treated with DAPT after PCI remains controversial and evidence of PFT-guided antiplatelet therapy for patients with ST-segment elevation myocardial infarction (STEMI) undergoing primary PCI is limited. METHODS: We analyzed 1,353 consecutive STEMI patients undergoing primary PCI. PFT was performed 72 hr postprocedure using a vasodilator-stimulated phosphoprotein assay. The primary endpoint of major adverse cardio-cerebral events (MACCEs) was defined as a composite of all-cause death, cardiac death, nonfatal myocardial infarction, target vessel revascularization, and ischemic stroke. Patients with high platelet reactivity (HPR) were randomized to receive an intensified antiplatelet strategy by switching from clopidogrel to ticagrelor (HPR switch group) or to continue on clopidogrel (HPR nonswitch group). One-year clinical outcomes were compared among the groups. RESULTS: The baseline clinical characteristics were comparable across all groups (all p > .05). At the 1-year clinical follow-up, the primary endpoint of MACCE was significantly higher in the HPR nonswitch group than in the non-HPR and HPR switch groups (19.49% vs. 10.20% or 8.57%, p < .05), which was mainly caused by higher mortality (14.87% vs. 4.51% or 5.71%, p < .05). Major bleeding events were comparable across the groups. CONCLUSIONS: In STEMI patients with HPR, identified by vasodilator stimulated phosphoprotein (VASP)-determined PFT, switching clopidogrel to ticagrelor could significantly improve 1-year clinical outcomes without increasing the risk of bleeding.


Assuntos
Plaquetas/efeitos dos fármacos , Terapia Antiplaquetária Dupla , Intervenção Coronária Percutânea , Inibidores da Agregação Plaquetária/administração & dosagem , Testes de Função Plaquetária , Infarto do Miocárdio com Supradesnível do Segmento ST/terapia , Idoso , Aspirina/administração & dosagem , Biomarcadores/sangue , Plaquetas/metabolismo , Moléculas de Adesão Celular/sangue , China , Clopidogrel/administração & dosagem , Substituição de Medicamentos , Terapia Antiplaquetária Dupla/efeitos adversos , Terapia Antiplaquetária Dupla/mortalidade , Feminino , Hemorragia/induzido quimicamente , Humanos , Masculino , Proteínas dos Microfilamentos/sangue , Pessoa de Meia-Idade , Intervenção Coronária Percutânea/efeitos adversos , Intervenção Coronária Percutânea/mortalidade , Fosfoproteínas/sangue , Inibidores da Agregação Plaquetária/efeitos adversos , Valor Preditivo dos Testes , Estudos Prospectivos , Recidiva , Medição de Risco , Fatores de Risco , Infarto do Miocárdio com Supradesnível do Segmento ST/diagnóstico , Infarto do Miocárdio com Supradesnível do Segmento ST/mortalidade , Ticagrelor/administração & dosagem , Fatores de Tempo , Resultado do Tratamento
20.
Clin Biochem ; 75: 78-82, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31770522

RESUMO

BACKGROUND: Till date, China has not issued industry standards for reference intervals (RIs) of pediatric blood coagulation indices. Here, we evaluated changes in the coagulation indices in the venous blood of healthy children aged 29 days to 12 years derived using the ACL Top 700 system and established appropriate RIs. METHODS: We analyzed venous blood from 1770 healthy children for five coagulation indices. RIs were established according to the Clinical and Laboratory Standards Institute C28-A3c guideline. RESULTS: The coagulation indices were grouped by age. For prothrombin time (PT) and international normalization ratio (INR), the RIs of infants and toddlers were identical; preschool children had the same RI as school-age children. Pediatric RIs for PT and INR were slightly lower than those for adults. The RIs of activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen (FIB) in childhood were divided into two groups by age (1 month to 1 year and 1-12 years). The RI of APTT in infants was the widest; the overall level of FIB in infants was the lowest; children's APTT and FIB RIs were lower than those of adults. The pattern of TT values and RI trends in childhood were similar to those of APTT. CONCLUSIONS: There were minor changes in the RIs of coagulation indices for children. The RIs of PT, INR, APTT, TT, and FIB must be grouped by age. The RIs of coagulation indices for children were different from those for adults; therefore, establishing separate RIs for children is necessary.


Assuntos
Testes de Coagulação Sanguínea/normas , Coagulação Sanguínea/fisiologia , Criança , Pré-Escolar , China/epidemiologia , Feminino , Voluntários Saudáveis , Humanos , Lactente , Recém-Nascido , Masculino , Valores de Referência
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