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1.
Front Vet Sci ; 8: 730701, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34760955

RESUMO

Pigs are the main host of Seneca Valley virus (SVV), previously known as Senecavirus A (SVA). Pigs affected by SVV have vesicles in the nose, hooves, and limp and may cause death in some severe cases. Occasionally, SVV has also been detected in mice, houseflies, environmental equipment, and corridors in pig farms. Moreover, it was successfully isolated from mouse tissue samples. In this study, an SVV strain (SVA/GD/China/2018) was isolated from a buffalo with mouth ulcers in the Guangdong province of China using seven mammalian cell lines (including BHK-21, NA, PK-15, ST, Vero, Marc-145, and MDBK). The genome of SVA/GD/China/2018 consists of 7,276 nucleotides. Multiple-sequence alignment showed that SVA/GD/China/2018 shared the highest nucleotide similarity (99.1%) with one wild boar-origin SVV strain (Sichuan HS-01) from the Sichuan province of China. Genetic analysis revealed that SVA/GD/China/2018 clustered with those porcine-origin SVV strains. To the best of our knowledge, this is the first report of SVV infection in buffalo, which might expand the host range of the virus. Surveillance should be expanded, and clinical significance of SVV needs to be further evaluated in cattle.

2.
Schizophr Bull ; 47(5): 1310-1319, 2021 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-33974073

RESUMO

Hypocretin (also called orexin) regulates various functions, such as sleep-wake rhythms, attention, cognition, and energy balance, which show significant changes in schizophrenia (SCZ). We aimed to identify alterations in the hypocretin system in SCZ patients. We measured plasma hypocretin-1 levels in SCZ patients and healthy controls and found significantly decreased plasma hypocretin-1 levels in SCZ patients, which was mainly due to a significant decrease in female SCZ patients compared with female controls. In addition, we measured postmortem hypothalamic hypocretin-1-immunoreactivity (ir), ventricular cerebrospinal fluid (CSF) hypocretin-1 levels, and hypocretin receptor (Hcrt-R) mRNA expression in the superior frontal gyrus (SFG) in SCZ patients and controls We observed a significant decrease in the amount of hypothalamic hypocretin-1 ir in SCZ patients, which was due to decreased amounts in female but not male patients. Moreover, Hcrt-R2 mRNA in the SFG was decreased in female SCZ patients compared with female controls, while male SCZ patients showed a trend of increased Hcrt-R1 mRNA and Hcrt-R2 mRNA expression compared with male controls. We conclude that central hypocretin neurotransmission is decreased in SCZ patients, especially female patients, and this is reflected in the plasma.


Assuntos
Hipotálamo/metabolismo , Receptores de Orexina/metabolismo , Orexinas/metabolismo , Córtex Pré-Frontal/metabolismo , Esquizofrenia/metabolismo , Adulto , Autopsia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Orexinas/sangue , Esquizofrenia/sangue , Fatores Sexuais
3.
J Clin Microbiol ; 59(8): e0007921, 2021 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-33952598

RESUMO

While China experienced a peak and decline in coronavirus disease 2019 (COVID-19) cases at the start of 2020, regional outbreaks continuously emerged in subsequent months. Resurgences of COVID-19 have also been observed in many other countries. In Guangzhou, China, a small outbreak, involving less than 100 residents, emerged in March and April 2020, and comprehensive and near-real-time genomic surveillance of SARS-CoV-2 was conducted. When the numbers of confirmed cases among overseas travelers increased, public health measures were enhanced by shifting from self-quarantine to central quarantine and SARS-CoV-2 testing for all overseas travelers. In an analysis of 109 imported cases, we found diverse viral variants distributed in the global viral phylogeny, which were frequently shared within households but not among passengers on the same flight. In contrast to the viral diversity of imported cases, local transmission was predominately attributed to two specific variants imported from Africa, including local cases that reported no direct or indirect contact with imported cases. The introduction events of the virus were identified or deduced before the enhanced measures were taken. These results show the interventions were effective in containing the spread of SARS-CoV-2, and they rule out the possibility of cryptic transmission of viral variants from the first wave in January and February 2020. Our study provides evidence and emphasizes the importance of controls for overseas travelers in the context of the pandemic and exemplifies how viral genomic data can facilitate COVID-19 surveillance and inform public health mitigation strategies.


Assuntos
COVID-19 , SARS-CoV-2 , África , Teste para COVID-19 , China/epidemiologia , Genômica , Humanos
4.
Mediators Inflamm ; 2021: 6635925, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33833618

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was initially identified in China and currently worldwide dispersed, resulting in the coronavirus disease 2019 (COVID-19) pandemic. Notably, COVID-19 is characterized by systemic inflammation. However, the potential mechanisms of the "cytokine storm" of COVID-19 are still limited. In this study, fourteen peripheral blood samples from COVID-19 patients (n = 10) and healthy donors (n = 4) were collected to perform the whole-transcriptome sequencing. Lung tissues of COVID-19 patients (70%) presenting with ground-glass opacity. Also, the leukocytes and lymphocytes were significantly decreased in COVID-19 compared with the control group (p < 0.05). In total, 25,482 differentially expressed messenger RNAs (DE mRNA), 23 differentially expressed microRNAs (DE miRNA), and 410 differentially expressed long noncoding RNAs (DE lncRNAs) were identified in the COVID-19 samples compared to the healthy controls. Gene Ontology (GO) analysis showed that the upregulated DE mRNAs were mainly involved in antigen processing and presentation of endogenous antigen, positive regulation of T cell mediated cytotoxicity, and positive regulation of gamma-delta T cell activation. The downregulated DE mRNAs were mainly concentrated in the glycogen biosynthetic process. We also established the protein-protein interaction (PPI) networks of up/downregulated DE mRNAs and identified 4 modules. Functional enrichment analyses indicated that these module targets were associated with positive regulation of cytokine production, cytokine-mediated signaling pathway, leukocyte differentiation, and migration. A total of 6 hub genes were selected in the PPI module networks including AKT1, TNFRSF1B, FCGR2A, CXCL8, STAT3, and TLR2. Moreover, a competing endogenous RNA network showed the interactions between lncRNAs, mRNAs, and miRNAs. Our results highlight the potential pathogenesis of excessive cytokine production such as MSTRG.119845.30/hsa-miR-20a-5p/TNFRSF1B, MSTRG.119845.30/hsa-miR-29b-2-5p/FCGR2A, and MSTRG.106112.2/hsa-miR-6501-5p/STAT3 axis, which may also play an important role in the development of ground-glass opacity in COVID-19 patients. This study gives new insights into inflammation regulatory mechanisms of coding and noncoding RNAs in COVID-19, which may provide novel diagnostic biomarkers and therapeutic avenues for COVID-19 patients.


Assuntos
COVID-19/sangue , COVID-19/genética , RNA/sangue , RNA/genética , SARS-CoV-2 , Adulto , Idoso , COVID-19/complicações , Estudos de Casos e Controles , Síndrome da Liberação de Citocina/sangue , Síndrome da Liberação de Citocina/etiologia , Síndrome da Liberação de Citocina/genética , Citocinas/biossíntese , Citocinas/genética , Feminino , Expressão Gênica , Humanos , Mediadores da Inflamação/sangue , Masculino , MicroRNAs/sangue , MicroRNAs/genética , Pessoa de Meia-Idade , Pandemias , Mapas de Interação de Proteínas/genética , RNA Longo não Codificante/sangue , RNA Longo não Codificante/genética , RNA Mensageiro/sangue , RNA Mensageiro/genética , Análise de Sequência de RNA , Transdução de Sinais , Sequenciamento Completo do Exoma , Adulto Jovem
5.
Chemosphere ; 270: 128637, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33097235

RESUMO

BACKGROUND: The adverse effects of TI exposure on pregnant women are still unclear, especially regarding the risk of gestational diabetes mellitus (GDM) Objective: We explored the association between maternal urinary Tl burden and the risk of GDM. METHODS: A subsample of 1789 pregnant women were enrolled who provided spot urine samples before the diagnostic 75-g oral glucose tolerance test. Urinary Tl concentration was measured using inductively coupled plasma mass spectrometry. Logistic regression and covariance analysis were carried out to estimate the association between Tl exposure and GDM risk. RESULTS: The median of urinary Tl concentration was 0.382 µg/L or 0.525 µg/g creatinine (CC-Tl). There were 437 (24.4%) participants who were diagnosed with GDM, and the urinary CC-Tl concentrations of pregnant women with GDM were higher than that of pregnant women without GDM [0.548 (0.402, 0.788) vs 0.518 (0.356, 0.724), p = 0.014]. After adjusting for the relevant covariates, an association between urinary Tl concentrations and GDM was found. In comparison to the pregnant women in the lowest quartile of urinary CC-Tl concentration, the pregnant women in the highest quartile had a higher risk of GDM [OR (95% CI) = 1.44 (1.03, 2.02), p-trend = 0.055]. If limited to the pregnant women without family history of diabetes, the results were still robust [OR (95% CI) = 1.59 (1.11, 2.30), p-trend = 0.012]. CONCLUSION: Urinary CC-Tl concentration was associated with GDM among Chinese pregnant women. Our findings provide evidence that moderately high Tl exposure may be a novel risk factor for pregnant women health.


Assuntos
Diabetes Gestacional , China , Estudos de Coortes , Diabetes Gestacional/induzido quimicamente , Diabetes Gestacional/epidemiologia , Feminino , Humanos , Exposição Materna/efeitos adversos , Gravidez , Fatores de Risco , Tálio
6.
Medicine (Baltimore) ; 99(49): e23098, 2020 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-33285685

RESUMO

To investigate the risk factors for progression of increased signal intensity (ISI) on T2W magnetic resonance imaging (MRI) and its prognostic value in patients with cervical spondylotic myelopathy (CSM).A total of 109 patients with CSM were included in this study. All the patients were treated with anterior cervical discectomy and fusion. MRI was performed for all 109 patients preoperatively and at the final follow-up. Radiological evaluation included ISI, anterior compression (AC) of dural and spinal cord, hyperintensity region (HR) at the involved level. Clinical data including Japanese Orthopedic Association (JOA) score, Neck Disability Index (NDI) score, and Visual Analogue Scale were collected and evaluated. Patients were divided into 2 groups according to ISI grades (Group A: no hyper-intensity; Group B: presence of ISI). Then all patients presented with ISI were divided into 2 subgroups based on the range of HR (Group B1: hyper-intensity diameter accounts for less than half of the spinal cord diameter at the involved level; Group B2, hyper-intensity diameter accounts for more than half of the spinal cord diameter at the involved level). AC, disease duration, age, and gender were analyzed as potential risk factors.Significantly better JOA and NDI scores were observed in Group A preoperatively and at the final follow-up, compared to Group B (P < .05). Disease duration was found significantly longer in patients with ISI (P < .05). Notably better JOA and NDI scores were noticed in Group B1 rather than Group B2 (P < .05). Logistical regression showed that disease duration was the only factor that significantly correlated with the progress of ISI (P < .001).CSM patients with ISI on T2W MR images had poorer surgical outcomes compared to others, while the increased range of HR may deteriorate preoperative neurological function. Moreover, patients with longer disease duration had greater risk of ISI in spinal cord.


Assuntos
Vértebras Cervicais/diagnóstico por imagem , Imageamento por Ressonância Magnética , Doenças da Medula Espinal/diagnóstico por imagem , Espondilose/diagnóstico por imagem , Adulto , Idoso , Vértebras Cervicais/cirurgia , Discotomia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos , Fatores de Risco , Doenças da Medula Espinal/etiologia , Doenças da Medula Espinal/cirurgia , Fusão Vertebral , Espondilose/complicações , Espondilose/cirurgia
7.
J Clin Lab Anal ; 34(10): e23590, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32960473

RESUMO

INTRODUCTION: The coronavirus disease (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which play important roles in regulating gene expression and are also considered as essential modulators during viral infection. The aim of this study was to elucidate the differential expression of miRNAs in COVID-19. METHODS: The total RNA was extracted and purified from the peripheral blood of ten patients with COVID-19 and four healthy donors. The expression levels of various miRNAs were detected by high-throughput sequencing, and correlation analysis was performed on the target genes that are primed by miRNAs. KEY FINDINGS: Compared with the healthy controls, 35 miRNAs were upregulated and 38 miRNAs were downregulated in the human patients with COVID-19. The top 10 genes were listed below: hsa-miR-16-2-3P,hsa-miR-5695,hsa-miR-10399-3P,hsa-miR-6501-5P,hsa-miR-361-3P,hsa-miR-361-3p, hsa-miR-4659a-3p, hsa-miR-142-5p, hsa-miR-4685-3p, hsa-miR-454-5p, and hsa-miR-30c-5p. The 10 genes with the greatest reduction were listed below: hsa-miR-183-5p, hsa-miR-627-5p, hsa-miR-941, hsa-miR-21-5p, hsa-miR-20a-5p, hsa-miR-146b-5p, hsa-miR-454-3p, hsa-miR-18a-5p, hsa-miR-340-5p, and hsa-miR-17-5p. Remarkably, miR-16-2-3p was the most upregulated miRNA, with a 1.6-fold change compared to that of the controls. Moreover, the expression of miR-6501-5p and miR-618 was 1.5-fold higher in the COVID-19 patients than in the healthy donors. Meanwhile, miR-627-5p was the most downregulated miRNA, with a 2.3-fold change compared to that of the controls. The expression of other miRNAs (miR-183-5p, miR-627-5p, and miR-144-3p) was reduced by more than 1.3-fold compared to that of the healthy donors. Cluster analysis revealed that all of the differentially expressed miRNA target genes were clustered by their regulation of cellular components, molecular functions, and biological processes. Importantly, peptidases, protein kinases, and the ubiquitin system were shown to be the highest enrichment categories by enrichment analysis. CONCLUSIONS: The differential miRNA expression found in COVID-19 patients may regulate the immune responses and viral replication during viral infection.


Assuntos
Infecções por Coronavirus , MicroRNAs , Pandemias , Pneumonia Viral , Adulto , Betacoronavirus , COVID-19 , Biologia Computacional , Infecções por Coronavirus/sangue , Infecções por Coronavirus/epidemiologia , Feminino , Perfilação da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , MicroRNAs/sangue , MicroRNAs/genética , MicroRNAs/metabolismo , Pessoa de Meia-Idade , Pneumonia Viral/sangue , Pneumonia Viral/epidemiologia , SARS-CoV-2 , Análise de Sequência de RNA , Adulto Jovem
8.
Inorg Chem ; 59(7): 4711-4719, 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32191452

RESUMO

High-capacity germanium-based anode materials are alternative materials for outstanding electrochemical performance lithium-ion batteries (LIBs), but severe volume variation and pulverization problems during charging-discharging processes can seriously affect their electrochemical performance. In addressing this challenge, a simple strategy was used to prepare the self-assembled GeOX/Ti3C2TX composite in which the GeOX nanoparticles can grow directly on Ti3C2TX layers. Nanoscale GeOX uniformly renucleates on the surface and interlayers of Ti3C2TX, forming the stable multiphase structure, which guarantees its excellent electrochemical performance. Electrochemical evaluation has shown that the rate capability and reversibility of GeOX/Ti3C2TX are both greatly improved, which delivers a reversible discharge specific capacity of above 1400 mAh g-1 (at 100 mA g-1) and a reversible specific capacity of 900 mAh g-1 after 50 cycles while it still maintains a stable specific capacity of 725 mAh g-1 at 5000 mA g-1. Furthermore, the composite exhibits an exceptionally superior rate capability, making it a good electrochemical performance anode for LIBs.

9.
Inorg Chem ; 58(12): 8169-8178, 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31141354

RESUMO

MoSe2 is a prospective anode material for Na-ion batteries because of its layered structure and high theoretical capacity, while the unsatisfied electrochemical performance limits its further development. Herein, we report MoSe2 nanosheets anchored on dual-heteroatoms functionalized graphene by a solvothermal method. The heteroatoms and carbon matrix coexist in the form of graphitic-N/pyridinic-N/pyrrolic-N and P-C/P═O bonds, which result in excellent electronic conductivity of the materials and provide abundant active sites for electrochemical process. Results indicated that organic intercalation increased the layer spacing of the materials to facilitate sodium-ion diffusion, and the in situ formed carbon networks improved the conductivity among the layers of the materials and alleviated volume expansion during the continued charge and discharge process. As an anode of Na-ion batteries, the nanosheets materials exhibited ultrahigh rate performance and deliver capacities of approximately 200 mAh g-1 at the current density of 10 A g-1. The ultrahigh-rate performance can be attributed to its unique nanosheets structure, the dual-heteroatoms functionalized graphene, and the considerable pseudocapacitive quality of the material.

10.
Int Urol Nephrol ; 50(5): 973-982, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29330775

RESUMO

PURPOSE: To assess a new and highly specific, but low-cost, easily performed and suitable for large-scale applications method for renal fibrosis (RF) diagnostics. METHODS: Thirty-five RF and twenty non-RF patients were enrolled in the study. An appropriate polyethylene glycol (PEG) was used to isolate urinary exosomes. The efficiency of isolation process was evaluated by the morphology and size observation, as well as the detection of specific markers (CD63, CD9). The expression level of exosomal miR-29c, miR-21 and the endogenous control snRNA-U6 were detected by qRT-PCR. The diagnostic potency of urinary exosomal miR-29c and miR-21 was estimated by the ROC method. Spearman's rank-order correlations analysis was used to assess the correlation between the miRNAs and clinical parameters, including pathological index. RESULTS: PEG-based method for isolation urinary exosome was effective and could be completed with a relatively low-speed centrifugal machine. Exosomal miR-29c and miR-21 were detected in all samples. The analysis of miRNAs in urinary exosomes revealed significant dys-regulation of miR-29c and miR-21 associated with RF. Exosomal miR-29c and miR-21 could predict degree of RF with AUC of 0.8333 and 0.7639 (P < 0.05). Correlation analysis showed that the level of miR-29c had a significant negative relationship with eGFR and the interstitial relative area. CONCLUSIONS: The PEG-based method for isolation urinary exosome is an inexpensive and easily performed approach. The application for cargo miRNA analysis is feasible. Urinary exosomal miR-29c may present a promising diagnostic approach.


Assuntos
Exossomos , Fibrose/diagnóstico , Fibrose/urina , Rim/patologia , MicroRNAs/urina , Polietilenoglicóis , Adulto , Idoso , Biomarcadores/urina , Estudos de Casos e Controles , Feminino , Fibrose/fisiopatologia , Taxa de Filtração Glomerular , Humanos , Rim/fisiopatologia , Nefropatias/fisiopatologia , Masculino , Pessoa de Meia-Idade , Curva ROC , Adulto Jovem
11.
Huan Jing Ke Xue ; 39(12): 5717-5722, 2018 Dec 08.
Artigo em Chinês | MEDLINE | ID: mdl-30628419

RESUMO

Five types of medical waste disposal technologies that are currently applied in our country were analyzed and compared such as the rotary kiln incineration method, pyrolysis method, high-temperature steam sterilization method, chemical disinfection method, and microwave disinfection method. The five methods were quantitatively evaluated and screened using an analytical hierarchy process (AHP). Based on this process, the ranking weights of optimal and suboptimal alternatives were studied using sensitivity analysis. The results show that the rotary kiln incineration and pyrolysis methods are presently the most widely used technologies for medical waste disposal in our country. The AHP shows that the comprehensive benefits of high-temperature steam sterilization in medical waste disposal are the best compared with the other four methods in terms of social, environmental, technological, and economic factors, followed by the chemical disinfection method. The sensitivity analysis shows that the change points of the guideline layer factors that lead to the change of the alternatives are the social factors (0.2100), environmental factors (0.3500), technical factors (0.1200), and economic factors (0.2400). Subcriteria factors have less influence on the weight ranking of the alternatives.


Assuntos
Desinfecção , Incineração , Eliminação de Resíduos de Serviços de Saúde/métodos , Micro-Ondas , Temperatura Alta , Resíduos de Serviços de Saúde , Vapor
12.
Environ Toxicol Pharmacol ; 54: 155-161, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28735146

RESUMO

Public concern is growing about the exposure to electromagnetic fields (EMF) and its effect on male reproductive health. Detrimental effect of EMF exposure on sex hormones, reproductive performance and sex-ratio was reported. The present study was designed to clarify whether paternal exposure to electromagnetic pulse (EMP) affects offspring sex ratio in mice. 50 male BALB/c mice aged 5-6 weeks were exposed to EMP daily for 2 weeks before mated with non-exposed females at 0d, 7d, 14d, 21d and 28d after exposure. Sex hormones including total testosterone, LH, FSH, and GnRH were detected using radioimmunoassay. The sex ratio was examined by PCR and agarose gel electrophoresis. The results of D0, D21 and D28 showed significant increases compared with sham-exposed groups. The serum testosterone increased significantly in D0, D14, D21, and D28 compared with sham-exposed groups (p<0.05). Overall, this study suggested that EMP exposure may lead to the disturbance of reproductive hormone levels and affect the offspring sex ratio.


Assuntos
Campos Eletromagnéticos , Razão de Masculinidade , Testosterona/sangue , Animais , Feminino , Hormônio Liberador de Gonadotropina/sangue , Hormônio Luteinizante/sangue , Masculino , Camundongos Endogâmicos BALB C , Reprodução
13.
Brain Res ; 1642: 10-19, 2016 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-26972535

RESUMO

A progressively expanded literature has been devoted in the past years to the noxious or beneficial effects of electromagnetic field (EMF) to Alzheimer׳s disease (AD). This study concerns the relationship between electromagnetic pulse (EMP) exposure and the occurrence of AD in rats and the underlying mechanisms, focusing on the role of oxidative stress (OS). 55 healthy male Sprague Dawley (SD) rats were used and received continuous exposure for 8 months. Morris water maze (MWM) test was conducted to test the ability of cognitive and memory. The level of OS was detected by superoxide dismutase (SOD) activity and glutathione (GSH) content. We found that long-term EMP exposure induced cognitive damage in rats. The content of ß-amyloid (Aß) protein in hippocampus was increased after long-term EMP exposure. OS of hippocampal neuron was detected. Western blotting and immunohistochemistry (IHC) assay showed that the content of Aß protein and its oligomers in EMP-exposed rats were higher than that of sham-exposed rats. The content of Beta Site App Cleaving Enzyme (BACE1) and microtubule-associated protein 1 light chain 3-II (LC3-II) in EMP-exposed rats hippocampus were also higher than that of sham-exposed rats. SOD activity and GSH content in EMP-exposed rats were lower than sham-exposed rats (p<0.05). Several mechanisms were proposed based on EMP exposure-induced OS, including increased amyloid precursor protein (APP) aberrant cleavage. Although further study is needed, the present results suggest that long-term EMP exposure is harmful to cognitive ability in rats and could induce AD-like pathological manifestation.


Assuntos
Doença de Alzheimer/metabolismo , Doença de Alzheimer/psicologia , Secretases da Proteína Precursora do Amiloide/efeitos da radiação , Peptídeos beta-Amiloides/efeitos da radiação , Precursor de Proteína beta-Amiloide/efeitos da radiação , Ácido Aspártico Endopeptidases/efeitos da radiação , Cognição/efeitos da radiação , Campos Eletromagnéticos , Estresse Oxidativo/efeitos da radiação , Secretases da Proteína Precursora do Amiloide/metabolismo , Peptídeos beta-Amiloides/metabolismo , Precursor de Proteína beta-Amiloide/metabolismo , Animais , Ácido Aspártico Endopeptidases/metabolismo , Cognição/fisiologia , Modelos Animais de Doenças , Hipocampo/metabolismo , Hipocampo/efeitos da radiação , Masculino , Aprendizagem em Labirinto/efeitos da radiação , Ratos , Ratos Sprague-Dawley
14.
Mol Med Rep ; 13(3): 2878-84, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26846193

RESUMO

Acquired resistance to epidermal growth factor inhibitors has been reported to be associated with cross­resistance to radiation. Paris Saponins (PSs) exert a wide range of pharmacological activities, including cell apoptosis induction, multidrug resistance inhibition, angiogenesis inhibition and tumor cell migration by modulating various signaling pathways. The present study aimed to investigate the radiosensitization effects of PSII, PSVI and PSVII in a gefitinib­resistant PC­9­ZD lung adenocarcinoma cell line, and the possible mechanism underlying their function. A clonogenic assay was performed to determine the effects of PS radiosensitization on the PC­9­ZD cell line. The cell cycle was analyzed by flow cytometry, and cell apoptosis was analyzed with Annexin V/propidium iodide and Hoechst staining. Protein expression levels were detected by western blotting. The results of the present study revealed a significant increase in PC­9­ZD cell line radiosensitivity following treatment with PSs. PSs induced G2/M cell cycle phase arrest and apoptosis of the irradiated PC­9­ZD cells. Notably, the expression levels of B cell lymphoma 2 (Bcl­2) were downregulated, and those of caspase­3, Bcl­2­associated X protein (Bax) and p21/Waf1/Cip1 were upregulated following treatment with PSs. The present results demonstrated that PSs induced radiosensitivity in gefitinib­resistant cells by inducing G2/M phase arrest and by enhancing the apoptotic response via the modulation of caspase­3, Bax, Bcl­2 and p21/Waf1/Cip1 expression.


Assuntos
Apoptose/efeitos dos fármacos , Diosgenina/análogos & derivados , Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos , Radiossensibilizantes/farmacologia , Saponinas/farmacologia , Adenocarcinoma/radioterapia , Adenocarcinoma de Pulmão , Antineoplásicos/farmacologia , Caspase 3/metabolismo , Linhagem Celular Tumoral , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Diosgenina/farmacologia , Resistencia a Medicamentos Antineoplásicos , Ensaios de Seleção de Medicamentos Antitumorais , Gefitinibe , Humanos , Neoplasias Pulmonares/radioterapia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Quinazolinas/farmacologia , Tolerância a Radiação/efeitos dos fármacos , Proteína X Associada a bcl-2/metabolismo
15.
Artigo em Chinês | MEDLINE | ID: mdl-30130007

RESUMO

Objective: To investigate the prevalence of parasitic infections in Lhasa, Tibet. Methods: The residents in Chengguan District, Dangxiong County, Dazi County and Qushui County were selected to participate in the study in 2014, using the stratified random sampling method. Infections with the soil-borne nematodes in feces were examined in triplicates using the Kato-Katz method. Further, the Enterobius vermicularis were examined using the cellophane anal swab method in children aged 3-12 years, and the intestinal protozoa were detected by iodine staining. The parasitic infection was analyzed by sex, age, and occupation. Comparisons were made using Chi-square test. Results: A total of 1 015 residents(39.3±18.2 years: age range, 1-89 years) participated in the study, including 391 males(38.6%) and 623 females(61.4%). Two hundred and forty-nine participants were positive for parasitic infections, with an overall infection rate of 24.5%(249/1 015). The infection rate was 21.7%(220/1 015) for Taenia sp., 0.2%(2/1 015) for Trichuris trichiura and E. vermicularis respectively, 4.1%(42/1 015) for Entamoeba coli, 0.4%(4/1 015) for Giardia lamblia, 0.2%(2/1 015) for Iodamoeba butschlii. No infection was found for other parasites. There was no significant sexual difference in the infection rate of Taenia (male 21.2%, 83/391; female 22.0%, 137/624), T. trichiura (male 0; female 0.3%, 2/624), E. vermicularis (male 0.3%, 2/391; female 0.2%, 1/624), E. coli (male 5.4%, 21/391; female 3.4%, 21/624), G. lamblia (male 0.8%, 3/391; female 0.2%, 1/624), or I. butschlii (male 0; female 0.3%, 2/624) (P>0.05). The Taenia sp. infection rate in the age groups of 0-17, 18-29, 30-39, 40-49, 50-59 and ≥ 60 years was 10.9%(18/165), 14.0%(18/129), 23.0%(43/187), 25.3%(61/241), 26.5%(41/155), and 28.3%(39/138), respectively, with significant differences between groups of 0-17 and 40-49 years and between groups of 50-59 and ≥ 60 years (P<0.01). The infection rate of E. coli, T. trichiura and I. butschlii was highest in the age group of 50-59 years(6.5%, 10/155; 0.7%, 1/155; 1.3%, 2/155 respectively). Among various occupations, farmers showed the highest infection rate for Taenia(44.5%, 147/330), E. coli(6.4%, 21/33), E. vermicularis (0.6%, 2/330) and I. butschlii(0.3%, 1/330); housewives had the highest infection rate for T. trichiura(2.5%, 1/40); and the students had the highest infection rate for G. lamblia (0.7%, 1/134). Conclusion: There are infections with various intestinal parasites in the participants of Lhasa City, among which taenia infection reaches 20.7% while others have a low level of infection.


Assuntos
Enteropatias Parasitárias/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Enterobius , Escherichia coli , Fazendeiros , Fezes , Feminino , Helmintos , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Nematoides , Prevalência , Solo , Inquéritos e Questionários , Teníase , Tibet/epidemiologia , Adulto Jovem
16.
Artigo em Chinês | MEDLINE | ID: mdl-26094412

RESUMO

OBJECTIVE: To evaluate the effect of malaria surveillance and control of Guangxi Zhuang Autonomous Region in 2013, and explore the suited surveillance and management of imported malaria cases, so as to provide the evidence for formulating the scientific control measures of imported malaria. METHODS: The endemic data and control measures of malaria in Guangxi in 2013 were collected and analyzed statistically. RESULTS: A total of 1 251 malaria cases were found in Guangxi in 2013, with 88.25% (1,104 cases) of falciparum malaria, 8.63% (108 cases) of vivax malaria, 0.64% (8 cases) of quartan malaria, 1.52% (19 cases) of ovale malaria, and 0.96% (12 cases) of mixed infection; 93.21% (1 166 cases) were off-farm workers; 96.56% (1,208 cases) were imported from Africa and mainly consisted of falciparum malaria cases; 3.44% (43 cases) were imported from southeast Asia and mainly consisted of vivax malaria cases. The cases of imported malaria were increasing and the infection rate in 2013 was increased by 464% compared with that in 2012. CONCLUSION: The imported malaria cases in Guangxi mainly come from Africa at present. Promoting the health education and professional skill of malaria control and treatment, as well as the diagnosis and treatment of the patients in early time are important measures to control the imported malaria.


Assuntos
Malária/epidemiologia , Malária/prevenção & controle , Vigilância de Evento Sentinela , Adolescente , Adulto , África , Idoso , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , População Rural , Viagem , Adulto Jovem
17.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 45(5): 767-71, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25341336

RESUMO

OBJECTIVE: To examine the changes in expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN) protein, p-PTEN protein and Bim (Bcl-2 interacting mediator of cell death) mRNA in the cortex of neonate rat brains with hypoxic-ischemic brain damage (HIBD) and to explore the mechanisms of neuroprotective effects of PTEN inhibition. METHODS: One hundred and twenty-eight neonate (10 days) SD rats were divided into four groups: hypoxia-ischemia (HI), sham control (Sham), bisperoxovanadium (bpv), and normal saline (NS) group. Rats in the HI group had their right common carotid arteries (CCA) exposed and ligated, and were then exposed to hypoxia in a chamber filled with 8% oxygen (balanced with nitrogen) for 2.5 h. Rats in the sham control group had their right CCA surgically exposed without ligation and exposure to hypoxia. Rats in the bpv treated group received intraperitoneal injections of bpv, 30 min before HI was induced. Instead of bpv, rats in the NS-treated group received intraperitoneal injections of NS. Cerebral cortex samples of the rats were collected 0.5 h and 24 h after hypoxia. Western blot was used to detect the protein expression of PTEN, p-PTEN and Bim. Real-Time PCR was used to detect the level of Bim mRNA. TUNEL staining was used to detect apoptotic cells. RESULTS: No significant changes of PTEN protein were observed in the rats exposed to HI. However, p-PTEN protein decreased in the rats exposed to HI (0.5 h and 24 h) compared with those exposed to sham surgery (P < 0.01). Compared with the sham controls, Bim mRNA and protein increased in the rats exposed to HI (0.5 h, P < 0.01) and then returned to the baseline level 24 h after HI. No significant changes of PTEN protein were observed in the bpv-treated rats. However, p-PTEN protein increased and Bim mRNA and protein decreased in the bpv-treated rats (0.5 h and 24 h, P < 0.01) compared with those in the HI group and NS-treated group. TUNEL positive cells also reduced in the bpv-treated rats (24 h, P < 0.01) compared with those in the HI group and NS-treated group. CONCLUSION: PTEN activities increase in the brains of neonate rats with hypoxic-ischemic brain damage. PTEN activity inhibition can decrease the level of pro-apoptotic protein Bim mRNA, leading to reduction of neuronal apoptosis.


Assuntos
Apoptose , Hipóxia-Isquemia Encefálica/enzimologia , Neurônios/citologia , PTEN Fosfo-Hidrolase/metabolismo , Animais , Animais Recém-Nascidos , Proteínas Reguladoras de Apoptose/metabolismo , Proteína 11 Semelhante a Bcl-2 , Encéfalo/patologia , Hipóxia-Isquemia Encefálica/patologia , Proteínas de Membrana/metabolismo , Neurônios/enzimologia , Proteínas Proto-Oncogênicas/metabolismo , RNA Mensageiro , Ratos , Ratos Sprague-Dawley
18.
Zhongguo Dang Dai Er Ke Za Zhi ; 16(1): 73-6, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-24461183

RESUMO

OBJECTIVE: To study the effect of ß8 expression on transforming growth factor ß1(TGF-ß1) activation in astrocytes with oxygen glucose deprivation (OGD). METHODS: Astrocytes were cultured and then subjected to OGD to generate hypoxia-ischemia (HI) model in vitro. Immunocytochemistry was used to detect the expression and distribution of ß8 in nomoxia cultured cells. ß8 protein expression was quantified by Western blot at 12 hours, 1 day and 2 days after OGD. Astrocytes and luciferase reporter cells (TMLC) were co-cultured. ß8 RNA interference system was established to specifically inhibit ß8 expression in cultured astrocytes. TGF-ß1 activation was then detected in the co-culture system. RESULTS: ß8 was mainly located in the cytoplasm and neurites of astrocytes. OGD resulted in increase of ß8 protein expression at 12 hours after reoxygenation in astrocytes, which was peaked at 1 day after reoxygenation. TGF-ß1 activation was in accordance with ß8 expression in astrocyte-TMLC co-culture system after reoxygenation. After the inhibition of ß8, TGF-ß1 activation was significantly reduced in all time points. CONCLUSIONS: The highly expressed ß8 plays important roles in the regulation of TGF-ß1 activation in neonatal rats with hypoxic-ischemic brain damage.


Assuntos
Astrócitos/metabolismo , Hipóxia-Isquemia Encefálica/metabolismo , Cadeias beta de Integrinas/fisiologia , Fator de Crescimento Transformador beta1/metabolismo , Animais , Feminino , Glucose/metabolismo , Masculino , Oxigênio/metabolismo , Ratos , Ratos Sprague-Dawley
19.
Chemosphere ; 97: 125-9, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24289932

RESUMO

A combined thermal desorption (TD)-molten salt oxidation (MSO) reactor system was applied to remediate the 1,2,3-trichlorobenzene (1,2,3-TCB) contaminated soil. The TD reactor was used to enrich the contaminant from soil, and its dechlorination of the contaminant was achieved in the MSO reactor. The optimum operating conditions of TD, and the effects of MSO reactor temperatures, additive amounts of the TCB on destruction and removal efficiency (DRE) of TCB and chlorine retention efficiency (CRE) were investigated. The reaction mechanism and pathway were proposed as well. The combined system could remediate the contaminated soil at a large scale of concentration from 5 to 25gkg(-1), and the DRE and CRE reached more than 99% and 95%, respectively, at temperatures above 850°C. The reaction emissions included C6H6, CH4, CO and CO2, and chlorinated species were not detected. It was found that a little increase in the temperature can considerably reduce the emission of C6H6, CH4, and CO, while the CO2 level increased.


Assuntos
Clorobenzenos/química , Recuperação e Remediação Ambiental/métodos , Sais/química , Poluentes do Solo/química , Solo/química , Cloro/análise , Cloro/química , Clorobenzenos/análise , Oxirredução , Poluentes do Solo/análise , Temperatura
20.
Zhongguo Dang Dai Er Ke Za Zhi ; 15(12): 1100-3, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24342207

RESUMO

Inhaled NO (iNO) has been shown to have beneficial effects on decreasing pulmonary inflammation, increasing function of surfactant and improving lung growth in prematurely born animal models. iNO has been gradually applied in the neonatal intensive care unit since its first use for persistent pulmonary hypertension (PPHN) in the early 1990's. Although many research findings have shown the benefits of iNO for hypoxic respiratory failure (HRF) of preterm infants, there is no certain evidence to support the routine use of iNO in premature infants. According to recent literature, the mechanism of iNO therapy, treatment scheme, iNO effectiveness and safety in premature infants were reviewed in this article, so as to provide bases for the clinical use of this treatment.


Assuntos
Hipóxia/complicações , Óxido Nítrico/administração & dosagem , Insuficiência Respiratória/tratamento farmacológico , Administração por Inalação , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Óxido Nítrico/efeitos adversos
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