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1.
J Nanosci Nanotechnol ; 20(2): 1109-1117, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383111

RESUMO

In recent years, the primary greenhouse gas (carbon dioxide) has caused a series of severe issues, such as global warming, climate change, etc. Therefore, CCU, CCS and CCUS technologies have been employed to reduce CO2 emissions. Through our developed "chemical vapor deposition integrated process (CVD-IP)" using carbon dioxide as the carbon source, CO2 could be catalytically activated and converted to high-value carbon nanotubes. In this work, methane and carbon dioxide has been applied to synthesize CNTs respectively to compare the difference between conventional CH4 CVD and CO2 CVD-IP technology using Ni-Mo bimetallic catalysts. In the conventional CH4 CVD technology, the carbon productivity and the thermal stability of CNTs could be improved by changing the Mo content of the catalyst, the better catalytic performance 4% Mo catalyst is selected as model catalyst to apply to CO2 CVD-IP technology. Moreover, when the weak oxidant CO2 is the only carbon source in the CVD-IP technology, the carbon yield is 22% and the carbon productivity is 1.98 g/gcat. TG curves and Raman spectroscopy display that the CNTs with better thermal stability and higher degree of graphitization are achieved. TEM confirms that the fewer wall numbers and defects of CNTs are obtained. These characterizations suggest that the high quality CNTs could be achieved by CO2 CVD-IP technology.

2.
J Nanosci Nanotechnol ; 20(4): 2567-2572, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492277

RESUMO

The crystal types of nanoparticles (NPs) are governed by many factors, such as temperature, pH, solvents, surface ligands, etc. In this work, ß-HgS and α-HgS NPs coated with captopril (Cap) have been synthesized conveniently in the same raw materials and similar methods just with different temperature at 4 °C and 50 °C, respectively. More interestingly, phase transition of HgS NPs from ß-crystal (cubic crystal) to α-crystal (hexagonal crystal) via rising temperature from 4 to 50 °C was confirmed by X-ray powder diffraction (XRD) and transmission electron microscopy (TEM). The size of α-HgS NPs grew to 6.7±1.3 nm in the process of phase transition, which was about twice of ß-HgS NPs (3.5±1.0 nm). Furthermore, compared with the obvious absorption peak of ß-HgS NPs at 688 nm, the absorption peak of α-HgS NPs had a giant blue-shifted to 470 nm in Ultraviolet and visible (UV-vis) spectra. In addition, the ß-HgS NPs had a fluorescence emission at 900 nm differing from α-HgS NPs without emission. The α-HgS NPs had three peaks at 215 nm, 251 nm and 318 nm, while the ß-HgS NPs had only a distinct peak at 215 nm in circular dichroism (CD) spectra. These results provide a novel insight for the controlling preparation of different crystal types of nanoparticles.

3.
J Nanosci Nanotechnol ; 20(4): 2578-2583, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492279

RESUMO

Quaternary colloidal CuIn0.7Ga0.3S2 nanocrystals were synthesized by hot injection method using oleylamine as single solvent. The structure, morphology and optical absorption properties of assynthesized CuIn0.7Ga0.3S2 nanocrystals were characterized in detail using X-ray powder diffraction, transmission electron microscopy and UV-vis spectrometry. The as-fabricated CIGSSe thin film was obtained by spin-casting as-synthesized colloidal CuIn0.7Ga0.3S2 nanocrystals after selenization. The classical pn heterojunction CIGSSe/CdS thin film solar cells displayed the 6.18% power conversion efficiency under AM1.5G illumination.

4.
Carbohydr Polym ; 227: 115335, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31590851

RESUMO

Thiolated chitosan (CS-NAC) was synthesized and the selected CS-NAC was used together with silk fibroin (SF) to produce dual network CS-NAC/SF hydrogels. The CS-NAC/SF solutions with formulated compositions were able to form hydrogels at physiological temperature and pH. Rheological measurements showed that elastic modulus of some CS-NAC/SF gels could reach around 3 kPa or higher and was much higher than their respective viscous modulus, indicating that they behaved like strong gels. Deformation measurements verified that CS-NAC/SF gels had well-defined elasticity. The optimized CS-NAC/SF gels exhibited jointly enhanced properties in terms of strength, stiffness and elasticity when compared to the gels resulted from either CS-NAC or SF. Examinations of dry CS-NAC/SF gels revealed that they were highly porous with well-interconnected pore features. Cell culture demonstrated that CS-NAC/SF gels supported the growth of chondrocytes while effectively maintaining their phenotype. Results suggest that these dual network gels have promising potential in cartilage repair.

5.
J Bone Joint Surg Am ; 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31567687

RESUMO

BACKGROUND: The Capanna technique involves the use of a vascularized fibular graft inlaid in a massive bone graft in intercalary reconstruction for diaphyseal long-bone defects caused by tumor resection. Allograft-host union time varies in different reports, and few studies have focused on the underlying factors affecting union time. The purpose of the present study was to analyze factors relevant to union time and to report complications of the Capanna technique. METHODS: We identified 60 patients who underwent segmental reconstruction with use of the Capanna technique following tumor resection (in the humerus in 10 patients, the femur in 33 patients, and the tibia in 17 patients). Multivariable linear multiple regression model analysis was performed with allograft-host osseous union time as the dependent variable. Union time was evaluated on radiographs. Independent variables included age, tumor site, adjuvant treatment, a previous surgical procedure, defect length, fixation method, and fibular viability. A retrieved specimen of the composite was histologically assessed. RESULTS: The mean defect length was 16 cm. All allografts and host bone united, with the mean time to union of 13 months (range, 6 to 27 months). Prolonged union time was associated with devitalization of the fibular graft (p < 0.001), use of chemotherapy (p = 0.031), and a previous surgical procedure (p = 0.048). Patient age (p = 0.742), amount of resection (p = 0.907), operative site (p = 0.508), and fixation method (p = 0.105) were not associated with union time. On histological analysis, we found that the allograft-host cortical junction was united by callus from both periosteum of the host bone and the fibula. CONCLUSIONS: The Capanna technique appears to be a reliable method for intercalary reconstruction with a low rate of complications. Devitalization of the transplanted fibula, chemotherapy, and a previous surgical procedure are adverse factors leading to prolonged union time. LEVEL OF EVIDENCE: Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.

6.
Chem Commun (Camb) ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31599287

RESUMO

A brand new europium(iii) ß-diketonate complex undergoes a single-crystal-to-single-crystal transformation via [2+2] cycloaddition after UV irradiation, triggering strong Eu(iii) red emission turn-on, which is highly photostable even after 50 hours of irradiation. A photo-patterning process is successfully conducted for security printing application in materials science.

7.
Pancreas ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31593021

RESUMO

OBJECTIVE: The objective of this study was to explore the exact relationship between the arterial radiomics score (rad-score) and lymph node (LN) metastasis in pancreatic ductal adenocarcinoma (PDAC). METHODS: A total of 225 patients with pathologically confirmed PDAC who underwent multislice computed tomography within 1 month of resection from December 2016 to August 2017 were retrospectively studied. For each patient, 1029 radiomics features of arterial phase were extracted, which were reduced using the least absolute shrinkage and selection operator logistic regression algorithm. Multivariate logistic regression models were used to analyze the association between the arterial rad-score and LN metastasis. RESULTS: Lymph node-negative and LN-positive patients accounted for 107 (47.56%) and 118 (52.44%) of the cohort, respectively. The rad-score, which consisted of 12 selected features of the arterial phase, was significantly associated with LN status (P < 0.05). Univariate analysis revealed that the arterial rad-score and T stage were independently and positively associated with risk of LN metastasis (P < 0.05). Multivariate analyses revealed a significant association between the arterial rad-score and the LN metastasis (P < 0.0001). Higher arterial rad-score was associated with LN metastasis (P for trend <0.0001). CONCLUSIONS: The arterial rad-score is independently and positively associated with the risk of LN metastasis in PDAC.

9.
J Am Heart Assoc ; 8(19): e012954, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31566101

RESUMO

Background Characterizing and assessing the prevalence, awareness, and treatment patterns of patients with isolated diastolic hypertension (IDH) can generate new knowledge and highlight opportunities to improve their care. Methods and Results We used data from the China PEACE (Patient-centered Evaluative Assessment of Cardiac Events) Million Persons Project, which screened 2 351 035 participants aged 35 to 75 years between 2014 and 2018. IDH was defined as systolic and diastolic blood pressure of <140 and ≥90 mm Hg; awareness as self-reported diagnosis of hypertension; and treatment as current use of antihypertensive medications. Of the 2 310 184 participants included (mean age 55.7 years; 59.5% women); 73 279 (3.2%) had IDH, of whom 63 112 (86.1%) were untreated, and only 6512 (10.3%) of the untreated were aware of having hypertension. When compared with normotensives, participants who were <60 years, men, at least college educated, had body mass index of >28 kg/m2, consumed alcohol, had diabetes mellitus, and prior cardiovascular events were more likely to have IDH (all P<0.01). Among those with IDH, higher likelihood of awareness was associated with increased age, women, college education, body mass index of >28 kg/m2, higher income, diabetes mellitus, prior cardiovascular events, and Central or Eastern region (all P<0.05). Most treated participants with IDH reported taking only 1 class of antihypertensive medication. Conclusions IDH affects a substantial number of people in China, however, few are aware of having hypertension and most treated participants are poorly managed, which suggests the need to improve the diagnosis and treatment of people with IDH.

10.
Biofactors ; 2019 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-31588658

RESUMO

The human intestine and its resident microbiota are known to play an essential role in the pathogenesis of primary obesity. However, a little is known of the gut microbiota profiles in individuals with obesity induced by glucocorticoid (GC) therapy. Here, we recruited 28 people with GC-induced obesity and 27 age- and gender-matched healthy controls, whose feces have been collected separately, to delineate the gut microbial characteristics in GC-induced obesity individuals. High-throughput Illumina sequencing targeting the V4 region of the 16S rRNA gene has been applied to analyze the structure of the intestinal microbiome. Gas chromatography was also used to measure levels of short-chain fatty acids (SCFAs), a subset of key gut microbial metabolites mainly produced by Bacteroidetes. We found dramatically decreased gut microbial diversity in individuals with GC-induced obesity. In addition, the bacterial communities of the GC-induced obesity group were enriched in Firmicutes (e.g., genus Streptococcus) and depleted in Bacteroidetes. Furthermore, SCFA content was decreased in GC-induced obesity status. Overall, this study conducted a case-control study with 55 participants in which we analyzed gut microbiota to develop intestinal microbial profiles of patients undergoing long-term treatment with GCs. Concomitantly, the level of SCFAs was also detected in those study participants.

11.
J Soc Psychol ; : 1-14, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31575333

RESUMO

Sense of entitlement can be defined as a pervasive sense that one deserves more and is entitled to more than others. Two studies examined the relationship between social class and sense of entitlement and how this relationship is moderated by system-justifying belief (SJB) in China. In Study 1, we conducted a survey among adults (N = 669) with results showing that social class was positively correlated with sense of entitlement for those endorsing SJB, but negatively correlated with sense of entitlement for those opposing SJB. In Study 2, we conducted an experiment among undergraduates (N = 128) with both social class and SJB being primed and the results replicated the pattern: the positive effect of primed higher social class on pay entitlement existed only for those primed with high SJB, and was dampened for those primed with low SJB. Therefore, higher-class individuals do not necessarily feel more entitled, and SJB may play an important role in shaping their sense of entitlement.

12.
Plant Cell ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31575723

RESUMO

Guanosine-3',5'-cyclic monophosphate (cGMP) is an important regulator in eukaryotes and cGMP-dependent protein kinase (PKG) plays a key role in perceiving cellular cGMP in diverse physiological processes in animals. However, the molecular identity, property, and function of PKG in plants remain elusive. This study has identified PKG from plants and characterized its role in mediating gibberellin (GA) response in rice. PKGs from plants are structurally unique with an additional type 2C protein phosphatase (PP2C) domain. Rice PKG possesses both protein kinase and phosphatase activities, and cGMP stimulates its kinase activity but inhibits its phosphatase activity. One of PKG's targets is GAMYB, a transcription factor in GA signaling, and the dual activities of PKG catalyze the reversible phosphorylation of GAMYB at Serine6, and modulate the nucleo-cytoplasmic distribution of GAMYB in response to GA. Loss of PKG impeded the nuclear-localization of GAMYB and abolished GAMYB function in GA response, leading to defects in GA-induced seed germination, internode elongation, and pollen viability. In addition to GAMYB, the results indicate that PKG has broader effects with multiple potential targets in particular involved in salt stress response.

13.
Environ Sci Technol ; 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31577137

RESUMO

Di-isononyl phthalate (DINP) is considered one of the main industrial alternatives to di(2-ethylhexyl) phthalate (DEHP), a well-known chemical with various toxic effects including the disruption with lipid metabolism. However, potential effects of DINP on lipid metabolism have rarely been investigated in mammals. Our study demonstrated that exposure of neonatal mice to DEHP and DINP at a daily dose of 0.048 or 4.8 mg/kg from postnatal day 0 (PND0) to PND21 caused non-monotonic, as well as tissue- and gender-specific, alterations of total fatty acid (FA) compositions in plasma, heart and adipose tissues. However, the patterns of disruption differed between DEHP- and DINP-treated groups. Based on targeted lipidomic analyses, we further identified gender-specific alterations of eight lipid classes in plasma following DEHP or DINP exposure. At the higher dose DEHP induced decreases in total phosphatidylcholines (PC) and phosphatidylinositol (PI) in females and increases in phosphatidylethanolamines (PE) and triglycerides (TG) in males. By contrast, DINP at the higher dose caused alterations of PE, PI, phosphatidylserines (PS) and cholesterols (CE) exclusively in male mice, but no changes were observed in female pups. Although the most significant dysregulation of lipid metabolism was often observed for the higher dose, the lower one could also disrupt lipid profiles and sometimes its effects may even be more significant than those induced by the higher dose. Our study for the first time identified tissue- and gender-specific disruptions of fatty acid compositions and lipidomic profiles in mice neonatally exposed to DINP. These findings question the suitability of DINP as a safe DEHP substitute and lay a solid foundation for further elucidation of its effects on lipid metabolism and underlying mechanisms.

14.
Medicine (Baltimore) ; 98(40): e17132, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31577704

RESUMO

BACKGROUND: Evidence-based guidelines are expected to provide clinicians with explicit recommendations on how to manage health conditions and bridge the gap between research and clinical practice. However, the existing practice guidelines(CPGs) vary in quality. This study aimed to evaluate the quality of CPGs of kidney cancer. METHODS: We systematically searched PubMed, Embase, China Biology Medicine disc, and relevant guideline websites from their inception to April, 2018. We identified CGPs that provided recommendations on kidney cancer; 4 independent reviewers assessed the eligible CGPs using the Appraisal of Guidelines for Research and Evaluation (AGREE II) instrument. The consistency of evaluations was calculated using intraclass correlation coefficients (ICC). RESULTS: A total of 13 kidney cancer CGPs were included. The mean scores for each AGREEII domain were as follows: scope and purpose-76.9%; clarity and presentation-76.4%; stakeholder involvement-62.8%; rigor of development-58.7%; editorial independence-53.7%; and applicability-49.4%. Two CPGs were rated as "recommended"; 8 as "recommended with modifications"; and 3 as "not recommended." Seven grading systems were used by kidney cancer CGPs to rate the level of evidence and the strength of recommendation. CONCLUSIONS: Overall, the quality of CPGs of kidney cancer is suboptimal. AGREE II assessment results highlight the need to improve CPG development processes, editorial independence, and applicability in this field. It is necessary to develop a standardized grading system to provide clear information about the level of evidence and the strength of recommendation for future kidney cancer CGPs.

15.
PLoS One ; 14(10): e0221892, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31577811

RESUMO

OBJECTIVES: To (i) investigate alterations in homotopic functional connectivity (hfc) in concussed patients relative to healthy controls (HC) and to (ii) interrogate whether hfc in concussed patients normalized during the recovery process. The relationship between symptom recovery and change in hfc was assessed using post-hoc analyses. METHODS: This study included 15 concussed patients (mean age = 39.1, SD = 10.1; sex: 13 females, 2 males) and 15 HC (mean age = 39.1, SD = 11.7; sex: 13 females, 2 males). Hfc patterns were interrogated using resting-state magnetic resonance imaging (rs-MRI) for 29 a priori selected pain-processing regions. Concussed patients underwent imaging at two time-points; at 1-month post-concussion (mean time following concussion: 28 days, SD = 9.5) and again at 5-months post-concussion (mean time following concussion: 121 days, SD = 13). At both time-points, symptoms associated with concussion were assessed using the Sports Concussion Assessment Tool (SCAT-3). RESULTS: Concussed patients had significantly weaker hfc in the following six regions 1-month post-concussion compared to HC: middle cingulate, posterior insula, middle occipital, spinal trigeminal nucleus, precentral and the pulvinar. There were no regions of significantly stronger hfc in concussed patients relative to HC. Longitudinally, patients showed significant symptom recovery 5-months post-concussion and had significant strengthening of hfc patterns in seven homotopic ROIs: middle cingulate, posterior insula, middle occipital, secondary somatosensory area, spinal trigeminal nucleus, precentral, and the pulvinar. Post-hoc analyses indicated a significant negative correlation between somatosensory functional connectivity strengthening and symptom severity. CONCLUSION: At 1-month post-concussion, patients had significantly weaker hfc in a number of pain-processing regions relative to HC. However, over a period of 5-months, region-pair connectivity showed significant recovery and normalization. Those patients with more successful symptom recovery at 5-months post-concussion had more functional somatosensory strengthening, suggesting an association between functional strengthening and post-concussion symptom recovery.

16.
Anal Chem ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31580650

RESUMO

We report here a modified aptamer selection method, magnetic cross-linking precipitation (MCP)-SELEX, for highly efficient library enrichment and aptamer isolation. MCP-SELEX isolates bound aptamers via highly efficient chemical cross-linking between amino groups of target proteins and activated carboxylic acid groups on magnetic beads (> 90% coupling efficiency). Importantly, MCP-SELEX avoids surface interferences in conventional target-fixed methods and substantially minimizes nonspecific binding. The enrichment efficiencies of MCP-SELEX for various proteins (PD-L1, ubiquitin, thrombin, and HSA) were all greatly higher than those of the conventional target-bound magnetic bead based-SELEX (MB-SELEX). Anti-thrombin aptamer with KD of 33 nM was successfully isolated by four rounds of MCP-SELEX. MCP-SELEX also enabled the efficient aptamer isolation by coupling with MB-SELEX or falling-off-SELEX. We identified structure-switching aptamers (SSAs) that specifically bind to HSA with low nanomolar dissociation constant via 3 rounds of MCP-SELEX and 1 round of falling-off-SELEX. Our HSA SSAs also have ~ 3-fold higher specificity against streptavidin relative to thrombin SSAs discovered through falling-off-SELEX only. The enriched library has ~78-fold higher signal-to-noise ratio (the number of DNAs eluted by 50 nM HSA divided by the number of DNAs self-dissociated in blank buffer) than that obtained by 4 rounds of direct falling-off-SELEX. We finally demonstrated the application of the selected SSA in fluorescent detection of HSA in urine with diagnostic required sensitivity and dynamic range. We expect that MCP-SELEX may be coupled with other selection methods to substantially accelerate aptamer discovery.

17.
J Biol Chem ; 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31586031

RESUMO

Piezo1 is a mechanosensitive cation channel with widespread physiological importance; however, its role in the heart is poorly understood. Cardiac fibroblasts help preserve myocardial integrity and play a key role in regulating its repair and remodeling following stress or injury. Here, we investigated Piezo1 expression and function in cultured human and mouse cardiac fibroblasts. RT-PCR experiments confirmed that Piezo1 mRNA in cardiac fibroblasts is expressed at levels similar to those in endothelial cells. Results from the Fura-2 intracellular Ca2+ assay validated Piezo1 as a functional ion channel that is activated by its agonist, Yoda1. Yoda1-induced Ca2+ entry was inhibited by Piezo1 blockers (gadolinium and ruthenium red) and was reduced proportionally by siRNA-mediated Piezo1 knockdown or in murine Piezo1+/- cells. Results from cell-attached patch-clamp recordings on human cardiac fibroblasts established that they contain mechanically activated ion channels and that their pressure responses are reduced by the Piezo1 knockdown. Investigation of Yoda1 effects on selected remodeling genes indicated that Piezo1 activation increases both mRNA levels and protein secretion of interleukin-6 (IL-6), a pro-hypertrophic and profibrotic cytokine, in a Piezo1-dependent manner. Moreover, Piezo1 knockdown reduced basal IL-6 expression from cells cultured on collagen-coated softer substrates. Multiplex kinase activity profiling, combined with kinase inhibitor experiments and phospho-specific immunoblotting, established that Piezo1 activation stimulates IL-6 secretion via the p38 mitogen-activated protein kinase downstream of Ca2+ entry. In summary, cardiac fibroblasts express mechanically activated Piezo1 channels coupled to secretion of the paracrine signaling molecule IL-6. Piezo1 may therefore be important in regulating cardiac remodeling.

18.
Med Sci Monit ; 25: 7459-7470, 2019 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31586039

RESUMO

BACKGROUND Depressive symptoms are a pervasive mental health problem in Chinese adolescents. The aim of this article was to systematically assess the trend of depressive symptoms in China among adolescents (1988 to 2018). MATERIAL AND METHODS A systematic and comprehensive literature search was conducted in both English and Chinese databases, including PubMed, EMBASE, Cochrane CENTRAL, CNKI, and Wan Fang Database, to identify relevant studies published between 1988 and 2018. Batteries of analyses in this meta-analysis were undertaken using Stata version 12.0 statistical software. RESULTS Sixty-two related reports involving 232 586 participants finally met our inclusion and exclusion criteria. The results suggest the prevalence of depressive symptoms has generally increased over time. The prevalence estimates before 2000 were 18.4% (95% CI, 14.5-22.3%), and were 26.3% (95% CI, 21.9-30.8%) after 2016. The pooled prevalence of depressive symptoms among children and adolescents was 22.2% (95% CI: 19.9-24.6%, I²=99.6%, p<0.001). More subgroup analyses classified by screening instrument, gender, and region were carried out in this meta-analysis. CONCLUSIONS Results of our meta-analysis suggest that depressive symptoms have become more prevalent among Chinese adolescents. This trend emphasizes the need for effective prevention strategies and greater availability of screening tools for this vulnerable population.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31589566

RESUMO

Toxoplasma gondii is one of protozoan parasites resulting in zoonosis, which can infect nearly all of warm-blooded hosts, including humans and raccoon dogs (Nyctereutes procyonoides). However, related reports on prevalence and genetic characterization of T. gondii strains in raccoon dogs were few in China. The aim of this study was to survey the prevalence and genetic characterization of T. gondii strains in domestic raccoon dogs from Jilin, Liaoning, and Hebei provinces, northern China. During April 2016 to November 2017, a total of 337 tissue samples collected from domestic raccoon dogs were detected with B1 gene using a nested PCR. And the positive samples were genotyped at 11 genetic markers (SAG1, 5'-and 3'-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus PCR-restriction fragment length polymorphism technology. Sixteen out of 337 sika deer (4.75%) were positive with B1 gene by nest PCR. Furthermore, four positive DNA samples were completely typed through further being genotyped, in which three samples were identified as ToxoDB Genotype #9, and one sample was confirmed as ToxoDB Genotype #10. The results of molecular detection not only revealed the existence of T. gondii in domestic raccoon dogs in Jilin, Liaoning, and Hebei for the first time, but also provided the information of genetic diversity. This study also indicated that ToxoDB Genotype #9 as a kind of potential reservoir for T. gondii transmission, may be main genotype in domestic raccoon dogs in China, posing a risk of infection in human health.

20.
Cells ; 8(10)2019 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-31590445

RESUMO

Cell-based assays for CDK8/19 inhibition are not easily defined, since there are no known cellular functions unique to these kinases. To solve this problem, we generated derivatives of 293 cells with CRISPR knockout of one or both of CDK8 and CDK19. Double knockout (dKO) of CDK8 and CDK19 together (but not individually) decreased the induction of transcription by NFκB (a CDK8/19-potentiated transcription factor) and abrogated the effect of CDK8/19 inhibitors on such induction. We generated wild type (WT) and dKO cell lines expressing luciferase from an NFκB-dependent promoter. Inhibitors selective for CDK8/19 over other CDKs decreased TNFα-induced luciferase expression in WT cells by ~80% with no effect on luciferase induction in dKO cells. In contrast, non-selective CDK inhibitors flavopiridol and dinaciclib and a CDK7/12/13 inhibitor THZ1 (but not CDK4/6 inhibitor palbociclib) suppressed luciferase induction in both WT and dKO cells, indicating a distinct role for other CDKs in the NFκB pathway. We used this assay to characterize a series of thienopyridines with in vitro bone anabolic activity, one of which was identified as a selective CDK8/19 inhibitor. Thienopyridines inhibited luciferase induction in the WT but not dKO cells and their IC50 values in the WT reporter assay showed near-perfect correlation (R2 = 0.98) with their reported activities in a bone anabolic activity assay, confirming that the latter function is mediated by CDK8/19 and validating our assay as a robust and quantitative method for CDK8/19 inhibition.

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