Oncology nurse competency in chimeric antigen receptor T-cell therapy: A qualitative study.

BACKGROUND: Chimeric antigen receptor (CAR) T-cell therapy is a promising therapeutic method in the field of tumor immunotherapy. An increasing number of patients are beginning to select CAR T-cell therapy in mainland China. It is characterized by a complex process, a long period of treatment, high individualization, quick disease status changes and unique side effects. Oncology nurses play a crucial role in the provision of CAR T-cell therapy. OBJECTIVE: To explore oncology nurses' competency in CAR T-cell therapy with guidance from the iceberg model. DESIGN: A descriptive qualitative study. SETTINGS: This study was conducted with nurses on lymphoma wards in three tertiary hospitals in China. PARTICIPANTS: A total of 13 nurses and 2 nursing managers were approached, and all of them took part in this study. METHODS: Data were collected face-to-face or via online video using a semistructured interview guide between November 2022 and February 2023 by the first author. The study was performed and reported following the Consolidated Criteria for Reporting Qualitative Research. Content analysis was used to analyze the data. RESULTS: Nursing competency in CAR T-cell therapy was identified as including four main categories encompassing 12 subcategories and 40 codes. The main categories were theoretical knowledge, operation skills, personality traits and motives. The subcategories were basic knowledge of chemotherapy, professional knowledge of CAR T-cell therapy, basic skills, professional skills, relevant discipline skills, communication/coordination ability, critical thinking, basic traits, professional personality, enthusiasm for the nursing profession, empathy with patients and motivation to promote professional development. CONCLUSIONS: Medical staff can develop a competency-based nurse training program to improve the professional competencies of oncology nurses in CAR T-cell therapy and meet patients' supportive needs for optimal care. Additionally, the findings may be helpful for building measurement standards to assess oncology nurses' performance.

Continuous crop rotation increases soil organic carbon stocks in river deltas: A 40-year field evidence.

River deltas, as important food production centers, support 66 % of the world's population, together with other coastal areas. However, agriculture in river deltas is negatively affected by soil salinization and agricultural intensification. Improving the soil carbon pool is a mutually beneficial solution for maximizing crop production and improving climate resilience to secure food production. In this study, long-term croplands in the Yellow River Delta (YRD), with a wheat-maize (WM) rotation system and a single cotton (SC) cropping system, were selected to explore the changes in soil organic carbon (SOC) stocks and the driving mechanisms at 0-20 cm depth from 1980 to 2020. We found that, over the past 40 years, the SOC stocks in WM and SC croplands had increased by 10.05 Mg C ha-1 and 7.44 Mg C ha-1, respectively. The Random forest model revealed that in the WM croplands, soil N stock and available K were the most important driving factors of SOC stocks, while in SC croplands, soil type and salinity were the most important driving factors of SOC stock dynamics. An increase in soil salinity to 2.0 ‰ caused a 17.5 % loss in SOC stocks in SC croplands. Our results show that, in the long run, croplands with a WM rotation system have stronger carbon sequestration potential. Depending on the planting system, promoting crop carbon input under high soil nutrients and affecting SOC decomposition by soil salinity are two different pathways of SOC sequestration in delta croplands. We propose that nutrient management and organic fertilizer application are crucial for increasing SOC stocks in the WM and SC croplands, respectively. This study confirms that it is of practical significance to take measures to promote soil carbon sequestration at the farmland scale and to provide scientific guidance for the sustainable development of river delta agriculture.

The ethnopharmacology, phytochemistry and pharmacology of the genus Hericium.

ETHNOPHARMACOLOGICAL RELEVANCE: Mushrooms in the genus Hericium are used as functional food and traditional medicines for a long history in East Asian countries such as China, India, Japan, and Korea. Some species of Hericium are called as monkey head mushroom (Houtougu) in China and Yamabushitake in Japan, which are traditionally considered as rare and precious health promoting food and medicinal materials for the treatment of dyspepsia, insomnia, chronic gastritis, and digestive tract tumors. THE AIM OF THE REVIEW: This review aims to summarize the ethnopharmacology and structural diversity of secondary metabolites from Hericium species, as well as the pharmacological activities of the crude extracts and pure compounds from Hericium species in recent years. MATERIALS AND METHODS: All the information was gathered by searching Scifinder, PubMed, Web of Science, ScienceDirect, Springer, Wiley, ACS, CNKI, Baidu Scholar, Google Scholar databases and other published materials (books and Ph.D. and M. Sc. Dissertations) using the keywords "Hericium", "Traditional uses", "Chemical composition", "Quality control" and "Pharmacological activity" (1971-May 2023). The species name was checked with https://www.mycobank.org/. RESULTS: The traditional uses of Hericium species were summarized, and 230 secondary metabolites from Hericium species were summarized and classified into six classes, mainly focusing on their chemical diversity, biosynthesis, biological activities. The modern pharmacological experiments in vivo or in vitro on their crude and fractionated extracts showed that the chemical components from Hericium species have a broad range of bioactivities, including neuroprotective, antimicrobial, anticancer, α-glucosidase inhibitory, antioxidant, and anti-inflammatory activities. CONCLUSIONS: The secondary metabolites discovered from Hericium species are highly structurally diverse, and they have the potential to be rich resources of bioactive fungal natural products. Moreover, the unveiled bioactivities of their crude extracts and pure compounds are closely related to critical human health concerns, and in-depth studies on the potential lead compounds, mechanism of pharmacological effects and pharmaceutical properties are clearly warranted.

Natural antibacterial membranes prepared using Schisandra chinensis extracts and polyvinyl alcohol in an environment-friendly manner.

Foodborne pathogens can cause food spoilage and lead to food safety issues. In recent years, food packaging has received a lot of attention. Traditional packaging membranes are non-biodegradable and remain in the environment for a long time. In this study, natural antimicrobial substances were extracted from Schisandra chinensis by a green extraction process using distilled water as the solvent, and the effects of different treatment on the antimicrobial activity of the extract were compared. At the same time, four types of Schisandra chinensis antimicrobial membranes were prepared using polyvinyl alcohol (PVA) as the substrate. The whole extraction and membrane preparation process did not involve organic solvents, making the process green and environment friendly. Material characterization included inverted biological microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), tensile strength test, pore size measurement, water uptake test, etc. Among them, no extract particles were observed with the naked eye on the surfaces of MⅡ and MⅣ. MⅡ has a uniformly transparent, nearly colorless morphology and is the most tensile. MⅣ surface is flat and smooth, the microstructure is dense and uniform. At the same time, the four types of membranes were tested against common pathogenic bacteria for 12 h, and the OD600 trend revealed the excellent antimicrobial activity of the membranes against S. aureus, MRSA, E. coli, and L. monocytogenes. The membranes could also be reused at least once. This study provides a new idea for preparing natural plant-based antimicrobial membranes.

ZnO/Au/GaN heterojunction-based self-powered photoelectrochemical Sensor for alpha-fetoprotein detection.

In recent years, the development of miniature and portable sensors has been a major focus of research. PEC self-powered sensors have emerged as a potential solution to the power supply issue, eliminating the need for external power supplies and operating without bias voltage. This study developed a ZnO/Au/GaN sensor for highly sensitive detection of alpha-fetoprotein (AFP). The sensor uses GaN substrates with nanogold films to provide an auxiliary bias voltage, promoting high photogenerated current density. Using ZnO/Au/GaN as a photoanode resulted in significantly higher photocurrent generated by the sensor compared to Au/GaN or ZnO/ITO alone. To enable selective detection of AFP, antibody modification of the ZnO nanorod arrays was employed. The linear range of the sensor response to AFP was determined to be 0.080-5.0 ng/mL, with an impressively low detection limit of 0.027 ng/mL (S/N = 3). These results demonstrate the potential of this self-powered sensor for detecting AFP content in human serum samples. Overall, this study presents a novel approach for developing highly sensitive and selective self-powered sensors for biomarker detection, which could facilitate early detection and clinical diagnosis of various types of cancer.

Individualized chemotherapy and efficacy analysis of hepatoblastoma in children.

PURPOSE: We aimed to assess the clinical utility of the mini patient-derived xenograft (MiniPDX) model in screening individualized chemotherapy regimens for pediatric hepatoblastoma. MATERIALS AND METHODS: We included 31 children with hepatoblastoma who had unsatisfactory decreases in alpha-fetoprotein levels during neoadjuvant chemotherapy or poor clinical control of recurrence with or without metastasis. We established a MiniPDX model using surgically resected tumor tissue specimens. The sensitivities of five chemotherapeutic regimens were tested to determine the one with the lowest tumor proliferation rate, which was then set as the experimental group. We compared the clinical characteristics and efficacy with those of conventional chemotherapy regimens. RESULTS: The median follow-up period for the experimental group was 27 months, with a complete remission (CR) rate of 80.64%. Among stage IV cases, there was a significant between-group difference in CR rate (experimental [73.68%] vs. control [37.5%]) and 3-year event-free survival rate (79.3% vs. 26.7%). The most effective individualized chemotherapy regimens were ifosfamide + pirarubicin + etoposide + carboplatin (54.84%), followed by pirubicin + cyclophosphamide + cisplatin (16.13%), ifosfamide + carboplatin + etoposide (12.90%), cisplatin + 5-fluorouracil + vincristine + adriamycin (12.90%), and vincristine + irinotecan + cyclophosphamide + cisplatin (3.23%). CONCLUSION: Using the MiniPDX model to screen individualized chemotherapy regimens for pediatric hepatoblastoma can significantly improve the CR rate.

Insight into key obstacles and technological strategy for enzymatic digestion of full cellulose fraction from poplar sawdust.

Lignocellulosic biomass mainly consists of hemicellulose, lignin, and cellulose, which differently affect the enzymatic digestibility of cellulose. As for the typical representative for inert woody biomass, three components of cellulose were proposed conceptually for poplar sawdust, i.e., active cellulose, inert cellulose, and resistant cellulose. Dilute sulfuric acid pretreatment, hydrogen peroxide-sulfuric acid delignification, and sulfuric acid-assisted glycerol swelling were, respectively, proven to break the three obstacle mechanisms that affect the cellulase of poplar. The removal of key obstacles improved the cellulase digestibility of poplar enzyme-hydrolyzed residues by 188.7 %, and glucose yield increased from 34.6 % to 99.9 %. Therefore, a total of 39.5 g glucose was obtained from 100 g poplar sawdust by integrating the above three technologies. This work presented insight into and removed the key obstacles to enzymatic digestibility of poplar cellulose and developed an integrated technology to effectively convert full cellulose fraction to glucose from woody biomass.

Short-term effects of ambient oxidation, and its interaction with fine particles on first-ever stroke: A national case-crossover study in China.

Stroke is a significant global cause of disability and death, and its burden has been on the rise, while ambient air pollution has been conclusively linked to stroke incidence. However, knowledge about effects of atmospheric oxidation on stroke and its interactions with fine particles (PM2.5) are still limited. In this study, we investigated the short-term effects of ambient NO2, O3, and their combined oxidation (Owt) on first-ever stroke, based on data from the China National Stroke Screening Survey (CNSSS) conducted from 2013 to 2015. We found significant association between ambient NO2 exposure at lag0 day with first-ever stroke, with a 13.1 % (95 % CI: 3.5 %, 23.6 %) increase in the first-ever stroke risk per 10 µg/m3 exposure. We also found a significant interaction between NO2 and PM2.5 (p < 0.05): first-ever stroke risk increased 23.8 % (95 % CI: 9.6 %, 39.8 %) per 10 µg/m3 NO2 exposure in population exposed to higher PM2.5 concentrations, while no significant association was found in population exposed to lower PM2.5 concentrations. The results of stratified analyses indicated that physical inactivity enhanced the detrimental effects of O3 and Owt exposure, while smoking and transient ischemic attack (TIA) history enhanced the detrimental effects of NO2 exposure. However, TIA history appeared to mitigate the adverse effects of O3 exposure. This study is helpful to better understand the impact of ambient oxidation on stroke, as well as its interaction with PM2.5, and has implications for policies and standards for atmospheric protection and governance.

Cas12a-based direct visualization of nanoparticle-stabilized fluorescence signal for multiplex detection of DNA methylation biomarkers.

The CRISPR-Cas12a RNA-guided complexes hold immense promise for nucleic acid detection. However, limitations arise from their specificity in detecting off-targets and the stability of the signal molecules. Here, we have developed a platform that integrates multiplex amplification and nanomolecular-reporting signals, allowing us to detect various clinically relevant nucleic acid targets with enhanced stability, sensitivity, and visual interpretation. Through the electrostatic co-assembly of the Oligo reporter with oppositely charged nanoparticles, we observed a significant enhancement in its stability in low-pollution environments, reaching up to a threefold increase compared to the original version. Additionally, the fluorescence efficiency was expanded by three orders of magnitude, broadening the detection range considerably. Utilizing a multiplex strategy, this assay can accomplish simultaneous detection of multiple targets and single-point indication detection of nine specific targets. This significant advancement heightened the sensitivity of disease screening and improved the accuracy of diagnosing disease-related changes. We tested this assay in a colorectal cancer model, demonstrating that it can identify DNA methylation features at the aM-level within 40-60 min. Validation using clinical samples yielded consistent results with qPCR and bisulfite sequencing, affirming the assay's reliability and potential for clinical applications.

Identifying the drivers of chlorophyll-a dynamics in a landscape lake recharged by reclaimed water using interpretable machine learning.

The water quality of lakes recharged by reclaimed water is affected by both the fluctuation of reclaimed water quality and the biochemical processes in the lakes, and therefore the main controlling factors of algal blooms are difficult to identify. Taking a typical landscape lake recharged by reclaimed water as an example and using the spatiotemporal distribution characteristics and correlation analysis of water quality indexes, we propose an interpretable machine learning framework based on random forest to predict chlorophyll-a (Chl-a). The model considered nutrient difference indexes between reclaimed water and lake water, and further used feature importance ranking and partial dependence plot to identify nutrient drivers. Results show that the NO3--N input from reclaimed water is the dominant nutrient driver for algal bloom especially at high temperatures, and the negative correlation between NO3--N and Chl-a in the lake water is the consequence of algal bloom rather than the cause. Our study provides new insights into the identification of eutrophication factors for lakes recharged by reclaimed water.

Seasonal variations of microplastics in surface water and sediment in an inland river drinking water source in southern China.

The aim of this study was to examine microplastic (size distribution of 0.05-5 mm) occurrence and distribution in drinking water source of XJ River during both flooding and dry periods. Surface water and sediment samples were collected from the CS City section of the river in August and December 2020. During the flooding period, microplastic abundances were observed at 0.72-18.6 (7.32 ± 2.36) items L-1 in surface water and 26.3-302 (150 ± 75.6) items kg-1 dry weight (dw) in sediment. In the dry period, abundances were slightly higher at 2.88-17.7 (11.0 ± 3.08) items L-1 and 27.0-651 (249 ± 182) items kg-1 dw, respectively. Microplastics were found in higher concentrations in urban areas and downstream of wastewater treatment plants, suggesting anthropogenic sources. The diversity in shapes, colors, and types of microplastics in surface waters and sediments indicates specialized enrichment processes and persistent sources of microplastic pollution. Approximately 60 % of the microplastic particles identified fall within the 50-100 µm range. Furthermore, a significant correlation was observed between these smaller-sized particles and the overall prevalence of microplastics. Fourier-transform infrared spectroscopy and scanning electron microscopy indicated that the microplastics had been subjected to weathering in the environment, contributing to the production of oxygen-containing functional groups and surface cleavage features. The utilization of energy dispersive spectroscopy revealed the presence of microplastics associated with various heavy metals, highlighting the intricate nature of microplastic pollution. Moreover, the high abundance of microplastics may pose a potential ecological risk to the aquatic environment of the XJ River. The results of this study demonstrate concerning levels of microplastics in the XJ River, despite its status as a high-quality water source.

Quantifying direct CO2 emissions from organic manure fertilizer and maize residual roots using 13C labeling technique: A field study.

Organic manure compost offers benefits like enhanced crop yield, improved soil health, and increased soil carbon storage. However, its application might elevate direct CO2 emissions from organic matter decomposition. Beyond manure compost, significant sources of CO2 emissions in agricultural settings are from residual roots and root exudates of pre-crops, and soil carbon. Quantifying the contribution of these sources to CO2 emissions is crucial for maximizing carbon reduction in crop-livestock systems, yet field studies have not assessed this contribution. Our study at the Yucheng field station in Shandong Province, China employed 13C labeling on summer maize to generate 13C-labeled manure compost and maize root, which is used to differentiate CO2 emissions from these sources. Our results revealed novel insights into the magnitude and patterns of CO2 emissions from these sources. The emission pattern of 13C-CO2 derived from manure compost, root and root exudates was similar, but the magnitude differed. Specifically, manure compost accounted for 5 % of the total CO2 emissions, while residual roots and root exudates contributed 2 % and 57 %, respectively, suggesting a higher labile carbon content in root exudates. The remaining 36 % of CO2 emissions was derived from the soil and other sources. CO2 emission factors were 6 % for manure compost, 12 % for roots, and 2 % for root exudates. By quantifying the direct emissions from manure compost, residual roots, root exudates, and soil, our study highlights the dominant role of managing root exudates in overall CO2 emissions. These findings can guide targeted carbon reduction strategies, emphasizing the importance of managing root exudates and understanding the relative innocuousness of manure compost applications in the context of CO2 emissions. This novel research quantifies the direct contribution of individual manure compost to CO2 emissions, providing valuable data for carbon cycle models and improving understanding of CO2 contributions from new carbon inputs.

Regulation of specific abnormal calcium signals in the hippocampal CA1 and primary cortex M1 alleviates the progression of temporal lobe epilepsy.

Temporal lobe epilepsy is a multifactorial neurological dysfunction syndrome that is refractory, resistant to antiepileptic drugs, and has a high recurrence rate. The pathogenesis of temporal lobe epilepsy is complex and is not fully understood. Intracellular calcium dynamics have been implicated in temporal lobe epilepsy. However, the effect of fluctuating calcium activity in CA1 pyramidal neurons on temporal lobe epilepsy is unknown, and no longitudinal studies have investigated calcium activity in pyramidal neurons in the hippocampal CA1 and primary motor cortex M1 of freely moving mice. In this study, we used a multi-channel fiber photometry system to continuously record calcium signals in CA1 and M1 during the temporal lobe epilepsy process. We found that calcium signals varied according to the grade of temporal lobe epilepsy episodes. In particular, cortical spreading depression, which has recently been frequently used to represent the continuously and substantially increased calcium signals, was found to correspond to complex and severe behavioral characteristics of temporal lobe epilepsy ranging from grade II to grade V. However, vigorous calcium oscillations and highly synchronized calcium signals in CA1 and M1 were strongly related to convulsive motor seizures. Chemogenetic inhibition of pyramidal neurons in CA1 significantly attenuated the amplitudes of the calcium signals corresponding to grade I episodes. In addition, the latency of cortical spreading depression was prolonged, and the above-mentioned abnormal calcium signals in CA1 and M1 were also significantly reduced. Intriguingly, it was possible to rescue the altered intracellular calcium dynamics. Via simultaneous analysis of calcium signals and epileptic behaviors, we found that the progression of temporal lobe epilepsy was alleviated when specific calcium signals were reduced, and that the end-point behaviors of temporal lobe epilepsy were improved. Our results indicate that the calcium dynamic between CA1 and M1 may reflect specific epileptic behaviors corresponding to different grades. Furthermore, the selective regulation of abnormal calcium signals in CA1 pyramidal neurons appears to effectively alleviate temporal lobe epilepsy, thereby providing a potential molecular mechanism for a new temporal lobe epilepsy diagnosis and treatment strategy.

Development of a sample preparation method for micro-proteomics analysis of the formaldehyde-fixed paraffin-embedded liver tissue samples.

Liver micro-proteomics based on the routinely used formaldehyde-fixed paraffin-embedded (FFPE) samples is valuable for innovative research, but the technical approach for sample preparation is often challenging. In this study, we aimed to develop a method for sample preparation for micro-proteomics on using the FFPE liver samples. We collected 2000 individual cells per batch from FFPE liver slices with laser capture microdissection and used them as test samples. We used the microscale fresh-frozen liver samples or HepG2 cells as control samples. For the FFPE samples, we first established a procedure for protein extraction. 2 h incubation at 95 °C in alkaline amine buffer supplemented with 4% sodium dodecyl sulfate allows improved production, efficiency, and quality of protein extraction. Then, we developed a dedicated protocol HDMSP for the micro-concentrated (<0.05 µg/µL) protein preparation for mass spectrometry (MS) based analysis, in which 2 µg/µL carboxyl magnetic beads and 70% acetonitrile are used to induce protein precipitation. For the 0.01 µg/µL protein control samples, protein recovery rate (PRR) by HDMSP is 72.1%, while the PRR is 5.9% if using a standard method solid phase-enhanced sample preparation. For the FFPE samples, the HDMSP PRR is 88.8%, and the subsequent MS analysis demonstrates increased depth, robustness, and quantitation accuracy for HDMSP relative to the control of in-gel digestion. Moreover, the physicochemical properties and subcellular location of the FFPE liver micro-proteome are comparable to those of the fresh-frozen control samples processed with filter-aided sample preparation (FASP). HDMSP is also comparable to FASP in terms of reproducibility and physicochemical properties in liver subcellular proteomes, and meanwhile reduces the sample preparation time by 15.9% and the experimental cost by 30.8%. Overall, the new method is simple and highly effective for preparing the microscale FFPE liver protein samples for MS analysis. This study provides a useful solution for FFPE liver micro-proteomics.

FZKA reverses gefitinib resistance by regulating EZH2/Snail/EGFR signaling pathway in lung adenocarcinoma.

ETHNOPHARMACOLOGICAL RELEVANCE: Fuzheng Kang-Ai (FZKA) decoction is mainly composed of 12 components with different types of herbs. In the last decade, FZKA has been used as an adjuvant treatment for lung cancer in clinical practice. Our previous studies have confirmed that FZKA shows a strong anti-cancer activity, significantly increases the clinical efficacy of gefitinib and reverses gefitinib resistance in non-small cell lung cancer (NSCLC). However, the molecular mechanism still needs to be further elucidated. AIM OF THE STUDY: The aim of this study was to investigate the role and mechanism by which FZKA inhibited the cell growth, proliferation and invasion of lung adenocarcinoma(LUAD) and reversed the acquired resistance of gefitinib for the therapy in LUAD. MATERIALS AND METHODS: Cell viability assay and EDU assay were used for detecting of cell viability and cell proliferation. Transwell assay was performed to measure cell invasion. Western Blot and qRT-PCR were used for protein and gene expression test. The gene promoter activity was determined by dul-luciferase reporter assay. The in situ expression of protein was measured by cell immunofluorescence. Stabilized cell lines were established for stable overexpression of EZH2. Transient transfection assay was used for gene silence and overexpression. Xenograft tumors and bioluminescent imaging were used for in vivo experiments. RESULTS: FZKA significantly inhibited the cell viability, proliferation and cell invasion of LUAD, the combination of FZKA and gefitinib had a great synergy on the above processes. Moreover, FZKA significantly decreased EZH2 mRNA and protein expression, FZKA reversed the resistance of gefitinib by down-regulation of EZH2 protein. ERK1/2 kinase mediated the down-regulation of EZH2 reduced by FZKA. In addition, FZKA decreased the expression of Snail and EGFR by decreasing EZH2. Overexpression of Snail and EGFR significantly reversed the effect of FZKA-inhibited cell invasion and cell proliferation. More important, the combination of FZKA and gefitinib enhanced the inhibitory effect on EZH2, Snail and EGFR proteins. Furthermore, the growth inhibition and reversal of gefitinib resistance induced by FZKA were further validated in vivo. Finally, the expression and clinical correlation of EZH2,EGFR and Snail in cancer patients were further validated using bioinformatics analysis. CONCLUSIONS: FZKA significantly suppressed tumor progression and reversed gefitinib resistance by regulating the p-ERK1/2-EZH2-Snail/EGFR signaling pathway in LUAD.

Hepatoprotective efficacy and interventional mechanism of JianPi LiShi YangGan formula in acute-on-chronic liver failure.

ETHNOPHARMACOLOGICAL RELEVANCE: Acute-on-chronic liver failure (ACLF) progresses rapidly with a high short-term death rate. Although JianPi LiShi YangGan formula (YGF) has been used to treat ACLF by managing inflammatory responses and reducing endotoxemia, hepatocyte injury, and mortality, the underlying mechanisms remain unclear. AIM OF THE STUDY: This study aims to investigate the potential mechanisms underlying the efficacy and protective benefits of YGF in mice with ACLF. MATERIALS AND METHODS: YGF composition was determined using high-performance liquid chromatography coupled with mass spectrometry. We constructed a mouse model of ACLF using carbon tetrachloride, lipopolysaccharide (LPS), and D-galactosamine (D-Gal), as well as an in vitro model of D-Gal/LPS-induced hepatocyte injury. The therapeutic effects of YGF in ACLF mice were verified using hematoxylin-eosin, Sirius red, and Masson staining, and by measuring serum alanine transaminase (ALT), aspartate transaminase (AST), and inflammatory cytokine levels. Mitochondrial damage in hepatocytes was evaluated using electron microscopy, while superoxide anion levels in liver tissue were investigated using dihydroethidium. Transcriptome analysis, immunohistochemistry, western blotting, and immunofluorescence assays were performed to explore the mechanisms underlying the ameliorative effects of YGF against ACLF. RESULTS: In mice with ACLF, YGF therapy partially decreased serum inflammatory cytokine levels, as well as hepatocyte injury and liver fibrosis. The livers of ACLF mice treated with YGF exhibited decreased mitochondrial damage and reactive oxygen species generation, as well as a decreased number of M1 macrophages and increased number of M2 macrophages. Transcriptome analysis revealed that YGF may regulate biological processes such as autophagy, mitophagy, and PI3K/AKT signaling. In ACLF mice, YGF promoted mitophagy and inhibited PI3K/AKT/mTOR pathway activation in hepatocytes. Meanwhile, the autophagy inhibitor 3M-A reduced the capacity of YGF to induce autophagy and protect against hepatocyte injury in vitro. In contrast, the PI3K agonist 740 Y-P suppressed the ability of YGF to control PI3K/AKT/mTOR pathway activation and induce autophagy. CONCLUSIONS: Together, our findings suggest that YGF mediates autophagy, tight junctions, cytokine generation, and other biological processes. In addition, YGF inhibits hepatic inflammatory responses and ameliorates hepatocyte injury in mice with ACLF. Mechanistically, YGF can promote mitophagy to ameliorate acute-on-chronic liver failure by inhibiting the PI3K/AKT/mTOR pathway.

Mechanism of Xiaojianzhong decoction in alleviating aspirin-induced gastric mucosal injury revealed by transcriptomics and metabolomics.

ETHNOPHARMACOLOGICAL RELEVANCE: Aspirin, as a first-line drug for the treatment of cardiovascular diseases, currently has high clinical usage. However, reports of aspirin-induced gastric mucosal injury are increasing. Xiaojianzhong decoction (XJZD), a classic traditional Chinese medicine formula, has been shown to alleviate gastric mucosal injury, although its potential mechanism of action requires further study. AIM OF THE STUDY: This study aimed to explore the effect and mechanism of XJZD in preventing aspirin-induced gastric mucosal injury. MATERIALS AND METHODS: Aspirin was used to induce damage in the morning, while XJZD was applied as an intervention in the afternoon. The compounds in the XJZD were analyzed by means of both high-performance liquid chromatography and ultra-performance liquid chromatography-tandem mass spectrometry. The overall condition of the aspirin-related gastric mucosal injury was evaluated. The expressions of inflammatory factors and tight-junction-related proteins and apoptosis were observed via immunohistochemistry and immunofluorescence. The expression levels of the apoptosis-related proteins were detected using Western blot. Transcriptomics was used to perform the integrative analysis of gastric tissues, which was then validated. Molecular dynamics was used to explore the interaction of key compounds within the XJZD with relevant targets. Finally, non-targeted metabolomics was used to observe any metabolic changes and construct a network between the differentially expressed genes and the differential metabolites to elucidate their potential relationship. RESULTS: XJZD can alleviate inflammation response, maintain the gastric mucosal barrier's integrity, reduce apoptosis and necroptosis levels, and promote the proliferation and repair of gastric mucosal tissues. Its mechanism of action may be related to the regulation of TNF-α signaling. Furthermore, molecular docking showed that the cinnamaldehyde within XJZD played an important role in its effects. In addition, XJZD can correct metabolic disorders, mainly regulating amino acid metabolism pathways. Moreover, six differential genes (Cyp1a2, Cyp1a1, Pla2g4c, etc.) were determined to alleviate both gastric mucosal injury and inflammation by regulating arachidonic acid metabolism, Tryptophan metabolism, etc. CONCLUSIONS: This study is the first to report that XJZD can inhibit necroptosis and gastric mucosal injury induced by aspirin, thereby revealing the complex mechanism of XJZD in relation to alleviating gastric mucosal injury from multiple levels and perspectives.

Synthetic of functionalized magnetic titanium-based metal-organic frameworks to efficiently remove Hg(â ¡) from wastewater.

The rapid development of process technology has led to rapid daily industrial production, which also produced a large amount of waste liquid. At the same time, the existing treatment technology cannot keep up with the demand, resulting in the malicious destruction of the environment by wastewater, especially mercury-containing wastewater was very harmful. Effective means of removing mercury ions need to be found. With magnetic ferric oxide as the core and titanium-based metal-organic frameworks as the shell, a new type of magnetic adsorbent (BTA-MIL-125(Ti)@Fe3O4) was synthesized. Materials were tested by multiple characterization methods and multiple sets of experiments. At optimal pH 6, the removal rate in 100 ppm Hg(Ⅱ) was as high as 95.8%. The theoretical adsorption capacity was 615 mg/L. Isothermal experiments, kinetic experiments and thermodynamic experiments have respectively verified that the material was a kind of adsorption material with self-emission heat based on chemical action and synergistic adsorption with Hill model. By simulating the immunity of a variety of ions (Cu, Zn, Mg, Ni, Cd), the material itself also exhibited a very high affinity for Hg(Ⅱ). The results of five high-cycle stable adsorption proved the repeatable stability of the material itself. Various characterization methods have also shown that nitrogen and sulfur-containing groups chelated with Hg(Ⅱ). All of the above was enough to show that the BTA-MIL-125(Ti)@Fe3O4 was a magnetic adsorption material with excellent performance and great prospects.

Natural antimicrobials from plants: Recent advances and future prospects.

Plant-based antimicrobial substances have emerged as promising alternatives to conventional antibiotics and preservatives. Although many review studies have been done in this field, many of these reviews solely focus on specific compounds from particular perspectives. This paper aims to provide a comprehensive review on the various types of plant-based antimicrobial substances, the extraction and purification processes, as well as the application and safety issues. Combining different natural plant-derived substances shows promise in enhancing antimicrobial activities. Moreover, despite the existence of various methods (e.g., microwave-assisted extraction, supercritical fluid extraction) to extract and purify antimicrobial substances, isolating pure compounds remains a laborious process. Sustainability issues should also be considered when developing extraction methods. Additionally, the extraction process generates a significant amount of plant waste, necessitating proper utilization to ensure economic viability. Lastly, not all plant-derived substances are safe, and further research is needed to investigate their toxicity before widespread application.

Interface-confined precise processing of Ag nanowire into AgPd-nanoparticle-sealed AgAu nanotroughs for boosting ethanol electrooxidation.

The functions of nanomaterials are closely linked with their fine structures and compositions. Precisely processing nanoparticles into morphology- and composition-varied nanostructures can a cutting-edge technology for producing complex nanostructures. Herein, we develop an interface-confined precise processing strategy towards toluene/water-interfacial Ag nanowires. Interfacial Ag nanowires are transformed into AgPd-nanoparticle-sealed AgAu nanotroughs with abundant AgPd/AgAu hetero-junctions (i.e., AgPdAu hetero-junction nanostructures). By adjusting the reaction conditions, composition-varied AgPdAu hetero-junction nanostructures can be obtained. The formation of AgPdAu hetero-junction nanostructures can be attributed to interface-confined precise etching towards Ag nanowires separately from the two subphases of the water and the toluene. Composition-optimized Ag13Pd67Au20 hetero-junction nanostructure shows satisfactory catalytic performance towards ethanol electrooxidation: ∼4 and 2 times in electrochemical-activity-surface-area-normalized activities; ∼6 and 5 times in mass-normalized activities higher than commercial Pd/C and Pt/C, respectively. The outstanding catalytic capability of Ag13Pd67Au20 may be attributed to optimized composition, porous nanostructures as well as abundant AgPd/AgAu hetero-junctions. This work demonstrates the feasibility of precisely processing interfacial nanoparticles, opening the way for creating morphology-well-defined composition-varied complex nanostructures.