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1.
Adv Mater ; : e2207955, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36659826

RESUMO

Propane/propene (C3 H8 /C3 H6 ) separation is essential in the petrochemical industry but challenging because of their similar physical and chemical properties. Adsorptive separation with C3 H8 -selective porous materials can energy-efficient produce high-purity C3 H6 , which is highly promising for replacing conventional cryogenic distillation but suffers from unsatisfactory performance. Herein, through the precise introduction of fluorinated functional groups into the confined pore space, a new fluorinated MOF (FDMOF-2) featuring the unique strong C3 H8 -trap was successfully constructed. FDMOF-2 exhibit an unprecedented C3 H8 capture capacity of 140 cm3 cm-3 and excellent C3 H8 /C3 H6 (1:1, v/v) selectivity up to 2.18 (298 K and 1 bar), thus setting new benchmarks for all reported porous materials. Single-crystal X-ray diffraction studies reveal that the tailor-made pore confinement in FDMOF-2 provided stronger and multiple attractive interactions with C3 H8 , enabling excellent binding affinities. Breakthrough experiments demonstrate that C3 H8 can be directly extracted from various C3 H8 /C3 H6 mixtures through FDMOF-2, affording an outstanding C3 H6 production (501 mmol L-1 ) with over 99.99% purity. Benefiting from the robust framework and hydrophobic ligands, the separation performance of FDMOF-2 can be well maintained even under 70% relative humidity conditions. The exceptional propylene separation productivity and ultrahigh structural stability make this MOF material a very promising candidate for this challenging separation process. This article is protected by copyright. All rights reserved.

2.
BMC Vet Res ; 19(1): 5, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624468

RESUMO

BACKGROUND: Fowl adenovirus is of major concern to the poultry industry worldwidely. In order to monitor the prevalent status of Fowl adenovirus in China, a total of 1920 clinical samples from apparently healthy birds in the 25 sites of poultry flocks, Slaughterhouse and living bird markets from 8 provinces in eastern China were collected and detected by PCR, sequencing, and phylogenetic analysis. RESULTS: The epidemiological survey showed that Fowl adenoviruses were detected in living bird markets, and circulating in a variety of fowl species, including chickens, ducks, goose and pigeons. Among the 1920 clinical samples, 166 samples (8.65%) were positive in the fowl adenovirus PCR detection. In this study, totally all the 12 serotypes (serotypes of 1, 2, 3, 4, 5, 6, 7, 8A, 8B, 9, 10 and 11) fowl adenoviruses were detected, the most prevalent serotype was serotype 1. Phylogenetic analysis indicated that 166 FAdVs of 12 serotypes were divided into 5 fowl adenovirus species (Fowl aviadenovirus A, B, C, D, E). CONCLUSIONS: In the epidemiological survey, 8.65% of the clinical samples from apparently healthy birds were positive in the fowl adenovirus PCR detection. Totally all the 12 serotypes fowl adenoviruses were detected in a variety of fowl species, which provided abundant resources for the research of fowl adenoviruses in China. The newly prevalent FAdV serotypes provides valuable information for the development of an effective control strategy for FAdV infections in fowls.


Assuntos
Infecções por Adenoviridae , Aviadenovirus , Doenças das Aves Domésticas , Animais , Doenças das Aves Domésticas/epidemiologia , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/veterinária , Epidemiologia Molecular , Filogenia , Galinhas , Aviadenovirus/genética , China/epidemiologia , Sorogrupo
3.
J Colloid Interface Sci ; 634: 630-641, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36549211

RESUMO

Designing efficient electrocatalysts to improve the overall water splitting and urea electrolysis efficiency for hydrogen generation can greatly solve the dilemma of energy shortage and environmental pollution. In this work, Co8FeS8@CoFe-MOF/NF heterojunction arrays were fabricated by embedding sulfides into the surface of metal-organic frameworks (MOFs) nanosheets as multifunctional electrocatalyst. The introduction of sulfide on CoFe-MOF/NF can not only adjust the electronic structure (electron-rich state) and change the surface properties (more hydrophilic), but also increase the active area to enhance the catalytic activity. The in situ Raman shows Co8FeS8@CoFe-MOF/NF is more easily to generate active species at low potentials and generates a higher content of active ß-MOOH phase than CoFe-MOF/NF. Therefore, the Co8FeS8@CoFe-MOF/NF exhibits excellent oxygen evolution reaction (OER) performance with an overpotential of 213 mV at 10 mA cm-2. Furthermore, when used as a urea oxidation reaction (UOR), only an ultralow potential of 1.311 V at 10 mA cm-2. More importantly, the assembled two-electrode drives overall water splitting and urea electrolysis with cell voltages of 1.62 V and 1.55 V at 10 mA cm-2, respectively. This work provides insights into the preparation of electrocatalysts with multifunctional heterostructure arrays for hydrogen production.

4.
Food Res Int ; 162(Pt A): 112012, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36461237

RESUMO

Contemporarily, there has been a growing consumption rate of areca nut (AN) products worldwide, despite the fact that both fresh and processed AN contain various hazardous ingredients, including toxic alkaloids and carcinogenetic aflatoxins. However, there is a dearth of toxicity and potential cancer risk information regarding toxic alkaloids and aflatoxins via consuming AN products. The present study conducted a comprehensive assessment of the combined hazardous effects of AN alkaloids and aflatoxins towards human digestive system, by methods of HPLC analysis, cell study and in vitro digestive system study. The results revealed a synergetic effect of arecoline and aflatoxins was on human gingival normal fibroblast cell of HGF-1 and a proliferation effect on human tongue squamous carcinoma cell of CAL-27. Specifically, the residual arecoline was as high as 91.08 µg·ml-1 in oral phase and 72.41 µg·ml-1 in gastric phase, which could be an evidence of oral cancer. More importantly, 25.93 % of AN products were contaminated with aflatoxins and the maximum value was three times the MRLs. Under these circumstances, the cytotoxic and MOE values raised a considerable health concern in terms of malignancy risk for children that consume processed AN product, especially compared to scenarios that involve adults and/or fresh AN samples. This study would give rise to a better understanding of the hazards associated with AN alkaloids and aflatoxins towards digestive system, and thus to predict the potential carcinogenic risk of AN products.


Assuntos
Aflatoxinas , Alcaloides , Adulto , Criança , Humanos , Areca/efeitos adversos , Arecolina/toxicidade , Aflatoxinas/toxicidade , Nozes , Alcaloides/toxicidade , Carcinogênese , Sistema Digestório
5.
Int J Biol Macromol ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36464187

RESUMO

In this work, natural biopolymer stemming from lignocellulosic peanut hull biomass was used as a green and low-cost adsorbent to eliminate anionic Congo red (CR) and Cr(VI) ions from aqueous sample. In order to enhance the removal performance, the lignocellulosic biopolymer was subjected to amino-modification by the graft copolymerization of (3-acrylamidopropyl) trimethylammonium chloride and N, N'-methylenebisacrylamide. The property of the prepared amino-functionalized biopolymer (AFB) was examined through FTIR, TG, SEM, particle size analysis, zeta potential determination and XPS. The adsorption efficacy of AFB for CR and Cr(VI) was tested at different pH, contact time and initial concentration. The kinetic, isotherm and thermodynamics investigations revealed that the uptakes of CR and Cr(VI) were the combination processes of chemical and physical interactions, and both endothermic in nature. The AFB exhibited good reusability without significant loss in adsorption capacity after five consecutive cycles. Mechanistic analysis indicated that the quaternary ammonium groups in AFB contributed a lot to the binding of anionic compounds through electrostatic attraction. In addition, n-π and hydrogen bonding while reduction and coordination were also responsible for the removal of CR and Cr(VI), respectively. The present study provides a favorable strategy for the removal of anionic contaminates in water by using green and sustainable lignocellulosic wastes.

6.
J Phys Chem A ; 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36469759

RESUMO

Chemical interaction between the tips and molecules is one of the main contributing mechanisms to tip-enhanced Raman spectroscopy (TERS). In this work, we calculate the TERS spectra of the biphenylene (BP) dimer at 13 nonequivalent tip sites by means of density functional theory and explore the influence of the TERS tip on vibrational mode characters and Raman intensity. The Raman intensity of the vibrational mode involving the antisymmetric stretching of tetra-rings is found to be specifically enhanced. We attribute this specific enhancement to the electronic sensitive atom vibrational character of the mode and infer that the vibrational strength of atoms can be tuned by the TERS tip. The results provide an intuitive interpretation on the effects of tip-induced electronic redistributions on specific vibrational modes in TERS and indicate the possibility to further improve the TERS resolution.

7.
Chembiochem ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36453606

RESUMO

1-Azasugar analogues of L-iduronic acid (L-IdoA) and D-glucuronic acid (D-GlcA) and their corresponding enantiomers were synthesized as potential pharmacological chaperones for mucopolysaccharidosis I (MPS I), a lysosomal storage disease caused by mutations in the gene encoding α-iduronidase (IDUA). The compounds were efficiently synthesized in nine or ten steps from D- or L-arabinose and the structures were confirmed by X-ray crystallographic analysis of key intermediates. All compounds were inactive against IDUA, although L-IdoA-configured 8 moderately inhibited ß-glucuronidase (ß-GLU). The D-GlcA-configured 9 was a potent inhibitor of ß-GLU and a moderate inhibitor of the endo-ß-glucuronidase heparanase. Co-crystallization of 9 with heparanase revealed that the endocyclic nitrogen of 9 forms close interactions with both the catalytic acid and catalytic nucleophile.

8.
Artigo em Inglês | MEDLINE | ID: mdl-36585582

RESUMO

Enrofloxacin (ENR) is a widely used veterinary fluoroquinolone antibiotic and is frequently detected in water environments. The degradation of ENR was examined utilizing molecular oxygen mediation using nanometer zero-valent copper (nZVC) as the catalyst in this work. The dosage of nZVC, initial pH, and reaction temperature were investigated as contributing factors to ENR degradation. The effects of Cl-, NO3-, SO42-, and humic acid on the degradation of ENR were investigated. The actual effects were evaluated using natural water. The reactive oxygen species (ROS) that participated in the reaction were identified, their generation mechanisms were elucidated, and the effects on ENR degradation were assessed. More emphasis was given to exploring ENR degradation and transformation pathways via analyses of HPLC-TOF-MS. Data showed that at 35 ℃, with an initial pH of 3 and exposed to air, an nZVC dose of 0.5 g·L-1 degraded ENR by 99.51% dramatically. HO• radicals were identified as the dominant ROS, and conversions among Cu0, Cu+, and Cu2+ played crucial roles in the generation of ROS. The destruction mechanism of ENR was speculated based on analyses of HPLC-TOF-MS results as the transformation of the piperazine ring into an oxidized state with a -COOH substitution with HO•, which caused ENR to be mineralized and converted into CO2, H2O, and [Formula: see text]. The ECOSAR program has been used to evaluate the toxicity of ENR and its degradation products, and oxidative degradation of nZVC significantly reduced its toxicity and increased its biodegradability. This research proposes a capable and practical method for removing ENR from water.

9.
J Cereb Blood Flow Metab ; : 271678X221147363, 2022 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-36545834

RESUMO

Hemodialysis patients exhibit anemia-related cerebral hyperperfusion and iron deposition (ID). However, the mechanisms underlying the pathology of cerebral ID are not clear. We investigated the role of cerebral blood flow (CBF) in the pathophysiology of cerebral ID in hemodialysis patients with anemia. This study recruited 33 hemodialysis patients with anemia and thirty-three healthy controls (HCs). All the subjects underwent quantitative susceptibility mapping (QSM) and arterial spin labeling (ASL) to measure ID and CBF in the cerebral nuclei. Furthermore, we evaluated lacunar infarction (LI), cerebral microbleeds, and total white matter hyperintensity volume (TWMHV). Hemodialysis patients with anemia showed significantly higher ID and CBF in some nuclei compared to the HCs after adjusting for age, sex, and total intracranial volume (TIV) [P < 0.05, false discovery rate (FDR) corrected]. CBF showed a positive correlation with ID in both patients and HCs after adjustments for age, gender, and TIV (P < 0.05, FDR corrected). Serum phosphorus, calcium, TWMHV, hypertension, and dialysis duration were independently associated with ID (P < 0.05). Hemoglobin, serum phosphorus, and LI were independently associated with CBF (P < 0.05). Mediation analysis demonstrated that CBF mediated the effects between hemoglobin and ID. Our study demonstrated that CBF mediated aberrant cerebral ID in hemodialysis patients with anemia.

10.
Dalton Trans ; 51(45): 17361-17367, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36322012

RESUMO

Designing highly active and durable oxygen evolution reaction (OER) electrocatalysts is indispensable for promoting the sluggish reaction kinetics of OER to achieve low overpotential. RuO2 is one of the representative oxygen evolution electrocatalysts in acid electrolytes, but it still faces the problem of poor stability. Herein, RuO2 nanocrystal with an ultra-small size of approximately 3.5 nm loaded on N-doped hierarchical porous carbon (NHC) is successfully synthesized by RuCl3 dipping, followed by an annealing process. It is found that NHC is assembled of porous nanosheets with a high specific surface area (BET up to 2107 m2 g-1), which could provide more active sites for RuO2 loading and enable decorated catalysts to be effectively utilized. Combining the advantages of porous NHC and the high electrocatalytic activity of RuO2, the optimized electrocatalyst RuO2/NHC3 achieves a low overpotential of 186 mV at 10 mA cm-2 for acidic water oxidation with a Tafel slope as low as 60 mV dec-1 and maintains excellent long-term stability throughout the 27 h chronopotentiometry test in 0.5 M H2SO4. The elevated acidic OER activity and stability can be attributed to the diminutive size, abundant oxygen vacancies, and enlarged electrochemically active surface area of RuO2/NHC3. This work could provide opportunities to explore efficient anodic electrocatalysts in acidic media.

11.
Chembiochem ; : e202200461, 2022 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-36265004

RESUMO

SARS-CoV-2 infects human epithelial cells through specific interaction with angiotensin-converting enzyme 2 (ACE2). In addition, heparan sulfate proteoglycans act as the attachment factor to promote the binding of viral spike protein receptor binding domain (RBD) to ACE2 on host cells. Though the rapid development of vaccines has contributed significantly to preventing severe disease, mutated SARS-CoV-2 strains, especially the SARS-CoV-2 Omicron variant, show increased affinity of RBD binding to ACE2, leading to immune escape. Thus, there is still an unmet need for new antiviral drugs. In this study, we constructed pharmacophore models based on the spike RBD of SARS-CoV-2 and SARS-CoV-2 Omicron variant and performed virtual screen for best-hit compounds from our disaccharide library. Screening of 96 disaccharide structures identified two disaccharides that displayed higher binding affinity to RBD in comparison to reported small molecule antiviral drugs. Further, screening PharmMapper demonstrated interactions of the disaccharides with a number of inflammatory cytokines, suggesting a potential for disaccharides with multiple-protein targets.

12.
Int J Mol Sci ; 23(19)2022 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-36232781

RESUMO

Weed interference in the crop field is one of the major biotic stresses causing dramatic crop yield losses, and the development of herbicide-resistant crops is critical for weed control in the application of herbicide technologies. To identify herbicide-resistant germplasms, we screened 854 maize inbreed lines and 25,620 seedlings by spraying them with 1 g/L glufosinate. One plant (L336R), possibly derived from a natural variation of line L336, was identified to have the potential for glufosinate tolerance. Genetic analysis validated that the glufosinate tolerance of L336R is conferred by a single locus, which was tentatively designated as ZmGHT1. By constructing a bi-parental population derived from L336R, and a glufosinate sensitive line L312, ZmGHT1 was mapped between molecular markers M9 and M10. Interestingly, genomic comparation between the two sequenced reference genomes showed that large scale structural variations (SVs) occurred within the mapped region, resulting in 2.16 Mb in the inbreed line B73, and 11.5 kb in CML277, respectively. During the fine mapping process, we did not detect any additional recombinant, even by using more than 9500 F2 and F3 plants, suspecting that SVs should also have occurred between L336R and L312 in this region, which inhibited recombination. By evaluating the expression of the genes within the mapped interval and using functional annotation, we predict that the gene Zm00001eb361930, encoding an aminotransferase, is the most likely causative gene. After glufosinate treatment, lower levels of ammonia content and a higher activity of glutamine synthetase (GS) in L336R were detected compared with those of L336 and L312, suggesting that the target gene may participate in ammonia elimination involving GS activity. Collectively, our study can provide a material resource for maize herbicide resistant breeding, with the potential to reveal a new mechanism for herbicide resistance.


Assuntos
Herbicidas , Aminobutiratos , Amônia/metabolismo , Glutamato-Amônia Ligase/metabolismo , Resistência a Herbicidas/genética , Herbicidas/metabolismo , Herbicidas/farmacologia , Melhoramento Vegetal , Transaminases/metabolismo , Zea mays/genética , Zea mays/metabolismo
13.
Materials (Basel) ; 15(20)2022 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-36295183

RESUMO

In this study, the effects of the catalysis of heavy metals on the pyrolysis of waste polyester textiles (WPTs) and the adsorption behaviors of the pyrolysis products of WPTs for Cr(VI) were explored. TG-DTG analysis indicated that the metal ions catalyzed the pyrolysis process by reducing the temperature of the decomposition of WPTs. The surface morphology and pore structure of the carbons were analyzed using SEM and BET. The results demonstrated that Zn-AC possessed the largest specific surface area of 847.87 m2/g. The abundant acidic functional groups on the surface of the activated carbons were proved to be involved in the Cr(VI) adsorption process via FTIR analysis. Cr(VI) adsorption experiments indicated that the adsorption process was more favorable at low pH conditions, and the maximum adsorption capacities of Zn-AC, Fe-AC, and Cu-AC for Cr(VI) were 199.07, 136.25, and 84.47 mg/g, respectively. The FTIR and XPS analyses of the carbons after Cr(VI) adsorption, combined with the adsorption kinetics and isotherm simulations, demonstrated that the adsorption mechanism includes pore filling, an electrostatic effect, a reduction reaction, and complexation. This study showed that metal salts catalyze the pyrolysis processes of WPTs, and the activated carbons derived from waste polyester textiles are promising adsorbents for Cr(VI) removal.

14.
EJNMMI Res ; 12(1): 70, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36307610

RESUMO

BACKGROUND: Programmed cell death 1 ligand 1(PD-L1) is overexpressed in many tumors. The radionuclide-labeled anti-PD-L1 monoclonal antibody can be used for imaging and therapy of PD-L1 overexpressing cancer. Here, we described 131I-labeled Atezolizumab (131I-Atezolizumab, targeting PD-L1) as a therapeutic agent for colorectal cancer with PD-L1 overexpression. METHODS: 131I-Atezolizumab was prepared by the Iodogen method. The expression levels of PD-L1 in different human colorectal cells were determined by flow cytometry, western blot and cell binding assay. The immunoreactivity of 131I-Atezolizumab to PD-L1 high-expressing cells was determined by immunoreactive fraction. The killing abilities of different concentrations of 131I-Atezolizumab on cells with high and low expression of PD-L1 were detected by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Cerenkov luminescence imaging (CLI) and radioimmunotherapy (RIT) of 131I-Atezolizumab were performed on two human colorectal cancer models. The distribution and tumor targeting of 131I-Atezolizumab were evaluated by imaging. Tumor volume and survival time were used as indicators to evaluate the anti-tumor effect of 131I-Atezolizumab. RESULTS: The expression level of PD-L1 in vitro determined by the cell binding assay was related to the data of flow cytometry and western blot. 131I-Atezolizumab can specifically bind to PD-L1 high-expressing cells in vitro to reflect the expression level of PD-L1. Immunoreactive fraction of PD-L1 high-expressing RKO cells with 131I-Atezolizumab was 52.2%. The killing ability of 131I-Atezolizumab on PD-L1 high-expressing cells was higher than that of low-expressing cells. CLI proved that the specific uptake level of tumors depends on the expression level of PD-L1. Effect of 131I-Atezolizumab RIT showed an activity-dependent tumor suppressor effect on RKO tumor-bearing mice with high PD-L1 expression. 131I-Atezolizumab (37 MBq) can improve the median survival time of mice (34 days), compared to untreated mice (27 days) (P = 0.027). Although a single activity(37 MBq) of 131I-Atezolizumab also inhibited the tumors of HCT8 tumor-bearing mice with low PD-L1 expression (P < 0.05), it could not prolong the survival of mice(P = 0.29). CONCLUSION: 131I-Atezolizumab can be used as a CLI agent for screening PD-L1 expression levels. It may be used as a radioimmunotherapy drug target for PD- L1 overexpressing tumors.

15.
Front Mol Neurosci ; 15: 927530, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36117918

RESUMO

Amyloid-ß (Aß) has long been considered as one of the most important pathogenic factors in Alzheimer's disease (AD), but the specific pathogenic mechanism of Aß is still not completely understood. In recent years, the development of structural biology technology has led to new understandings about Aß molecular structures, Aß generation and clearance from the brain and peripheral tissues, and its pathological toxicity. The purpose of the review is to discuss Aß metabolism and toxicity, and the therapeutic strategy of AD based on the latest progress in molecular structures of Aß. The Aß structure at the atomic level has been analyzed, which provides a new and refined perspective to comprehend the role of Aß in AD and to formulate therapeutic strategies of AD.

16.
J Am Chem Soc ; 144(37): 17033-17040, 2022 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-36069372

RESUMO

Propane/propylene separation is one of the most challenging and energy-consuming but most important tasks in the petrochemical industry. Herein, a stable hydrogen-bonded organic framework (HOF-FJU-1) was tailor-made for highly efficient propylene separation from binary C3H6/C3H8 and even seven component CH4/C2H4/C2H6/C3H6/C3H8/CO2/H2 mixtures. The temperature-controllable diffusion channels in HOF-FJU-1 have enabled the porous material to completely exclude propane to reach high-performance propylene purification under energy-efficient operation conditions. Single-crystal structural analysis revealed that the well-matched pore aperture of HOF-FJU-1 can exactly accommodate propylene molecules via multiple intermolecular interactions, exhibiting a very high propylene/propane selectivity of 616 at 333 K. The propylene purity and productivity are over 99.5% and 30.2 L kg-1 from the binary C3H6/C3H8 (50/50) mixture at 333 K. Through a follow-up column separation of C3H6/C2H4 at 353 K, not only high-purity propylene (99.5%) but also ethylene (98.3%) can be readily collected from the seven component CH4/C2H4/C2H6/C3H6/C3H8/CO2/H2 (31/10/25/10/10/1/13) cracking gas mixtures. The great potential of HOF-FJU-1 for the industrial propylene separation process has been further supported by the high stability of this porous material under different environments and straightforward processibility and regeneration feasibility.


Assuntos
Dióxido de Carbono , Propano , Alcenos , Etilenos , Hidrogênio , Propano/química
17.
Front Oncol ; 12: 975758, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091106

RESUMO

Gastrointestinal cancers are a group of cancers occurred in gastrointestinal tissues with high morbidity and mortality rate. Although numerous studies were conducted on the investigation of gastrointestinal cancers, the real mechanisms haven't been discovered, and no effective methods of prevention and treatment of gastrointestinal cancers have been developed. Autophagy, a vital catabolic process in organisms, have been proven to participate in various mechanisms and signaling pathways, thus producing a regulatory effect on various diseases. The role of autophagy in gastrointestinal cancers remains unclear due to its high complexity. In this review, firstly, the biological features of autophagy will be introduced. Secondly, the role of autophagy in three popular gastrointestinal cancers, namely esophageal cancer, gastric cancer, and colorectal cancer will be described and discussed by reviewing the related literature. We aimed to bring novel insights in exploring the real mechanisms for gastrointestinal cancers and developing effective and efficient therapeutic methods to treat gastrointestinal cancers.

18.
Molecules ; 27(18)2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36144529

RESUMO

A series of natural deep eutectic solvents (NADESs) were prepared with choline chloride, betaine, and a variety of natural organic acids in order to find new environmentally-friendly green solvents to replace the traditional solvents. The NADESs were employed to extract flavonoids from Potentilla fruticosa L. (PFL) with the help of ultrasound. The eutectic solvent diluted with an appropriate amount of water improved the extraction ability of flavonoids due to the decrease of solution viscosity. The microstructure of the raw sample and the samples subjected to ultrasonic bath in different solutions were observed using scanning electron microscope (SEM) to determine the role of the NADESs in the extraction process. The DPPH method and glucose consumption method were used to study the antioxidant and hypoglycemic ability of flavonoid compounds in PFL. Single factor method and response surface methodology (RSM) were designed to analyze the effects of three extraction parameters, including solvent/solid ratio, ultrasonic power, and extraction time, on the extraction yield, antioxidant capacity, and hypoglycemic capacity, and the corresponding second-order polynomial prediction models were established. The optimal extraction conditions for the maximum extraction yield, antioxidant capacity, and hypoglycemic capacity were predicted by RSM, and the reliability of RSM simulation results was verified by a one-off experiment.


Assuntos
Potentilla , Rosaceae , Antioxidantes/farmacologia , Betaína , Colina/química , Solventes Eutéticos Profundos , Flavonoides/química , Glucose , Hipoglicemiantes , Reprodutibilidade dos Testes , Solventes/química , Água
19.
Life (Basel) ; 12(9)2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36143374

RESUMO

Potentilla bifurca is widely distributed in Eurasia, including the Tibetan Plateau. It is a valuable medicinal plant in the Tibetan traditional medicine system, especially for the treatment of diabetes. This study investigated the functional gene profile of Potentilla bifurca at different altitudes by RNA-sequencing technology, including de novo assembly of 222,619 unigenes from 405 million clean reads, 57.64% of which were annotated in Nr, GO, KEGG, Pfam, and Swiss-Prot databases. The most significantly differentially expressed top 50 genes in the high-altitude samples were derived from plants that responded to abiotic stress, such as peroxidase, superoxide dismutase protein, and the ubiquitin-conjugating enzyme. Pathway analysis revealed that a large number of DEGs encode key enzymes involved in secondary metabolites, including phenylpropane and flavonoids. In addition, a total of 298 potential genomic SSRs were identified in this study, which provides information on the development of functional molecular markers for genetic diversity assessment. In conclusion, this study provides the first comprehensive assessment of the Potentilla bifurca transcriptome. This provides new insights into coping mechanisms for non-model organisms surviving in harsh environments at high altitudes, as well as molecular evidence for the selection of superior medicinal plants.

20.
Nanomaterials (Basel) ; 12(17)2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36079965

RESUMO

Noble metal nanostructures can produce the surface plasmon resonance under appropriate photoexcitation, which can be used to promote or facilitate chemical reactions, as well as photocatalytic materials, due to their strong plasmon resonance in the visible light region. In the current work, Ag/Au nanoislands (NIs) and Ag NIs/Au film composite systems were designed, and their thermocatalysis performance was investigated using luminescence of Eu3+ as a probe. Compared with Ag NIs, the catalytic efficiency and stability of surface plasmons of Ag/Au NIs and Ag NIs/Au film composite systems were greatly improved. It was found that the metal NIs can also generate strong localized heat at low temperature environment, enabling the transition of NaYF4:Eu3+ to Y2O3: Eu3+, and anti-oxidation was realized by depositing gold on the surface of silver, resulting in the relative stability of the constructed complex.

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