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1.
J Orthop Surg Res ; 14(1): 464, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31881893

RESUMO

BACKGROUND: Follow-up after artificial joint replacement greatly helps achieve surgical outcomes. Mobile internet technology and mobile terminal equipment may increase the effectiveness of artificial joint replacement. However, only a few studies have evaluated the effectiveness of this technology. We aimed to analyze the reasons and outcomes of patients who used the instant messaging platform after undergoing artificial joint replacement. METHODS: Among the 548 cases of arthroplasty (250 hips, 298 knees) performed between December 2015 and June 2018 in the Department of Joint Surgery of our institution; 358 (164 hip joints, 194 knee joints) participated in instant messaging platform consultation, whereas the remaining 190 (86 hip joints, 104 knee joints) participated in traditional telephone consultation, as a control group. Follow-up time was from December 2015 to August 2018 (follow-up period was 2-32 months). Data on age, sex, type of surgery, date of surgery, date of discharge, and length of hospital stay were collected from electronic medical records. RESULTS: We analyzed the consultation contents of 358 patients who participated in instant messaging platform consultation. Counseling was mainly related to pain (13.6%), appointment review (12.4%), activity problems (10.5%), and incision problems (8.9%). Most problems were resolved through online guidance, with 8.4% of patients requiring only outpatient treatment and 2.5% of patients requiring rehospitalization. A total of 190 patients were followed up through traditional telephone consultation; 6.8% of patients required outpatient department treatment and 7.4% were eventually re-admitted. CONCLUSION: The instant messaging platform consultation service effectively informs patients of potential postoperative problems and helps resolve them. It allows early detection and management of postoperative adverse events, including problems related to medication, wound, and activity, thereby effectively reducing readmission rate.

2.
Sci Rep ; 7: 46906, 2017 10 20.
Artigo em Inglês | MEDLINE | ID: mdl-29052595

RESUMO

This corrects the article DOI: 10.1038/srep16768.

3.
PLoS One ; 12(9): e0184456, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28886133

RESUMO

Machilus pauhoi Kanehira is an important timber species in China. A provenance trial was recently set up to evaluate the growth performance of trees from different localities, with the aim of designing seed transfer guidelines. Here, we tested twelve nuclear microsatellite markers derived from other species of the Lauraceae family and investigated population genetic structure in M. pauhoi. Both the number of observed alleles per locus (Na) and the polymorphic information content (PIC) significantly decreased against the latitude, but showed an insignificant decrease against the longitude. Heterozygosity (Ho) and gene diversity (h) exhibited a weak correlation with geographic location. Private alleles were present in multiple populations, and a moderate level of population genetic differentiation was detected (Gst = 0.1691). The joint pattern of genetic diversity (Na, PIC, Ho, and h) suggests that general northeastward dispersal led to the current distribution of M. pauhoi. Significant but weak effects of isolation-by-distance (IBD) occurred, implicating the mountain ranges as the major barrier to gene flow. Both STRUCTURE and hierarchical clustering analyses showed three distinct groups of populations related to the physical connectivity among mountain ranges. A priority in designing genetic conservation should be given to the populations at the southwest side of the species' distribution. This conservation strategy can also be combined with the pattern of adaptive genetic variation from the provenance trial for comprehensive genetic resource management of native M. pauhoi.


Assuntos
Lauraceae/classificação , Lauraceae/genética , Filogenia , Filogeografia , Alelos , China , Meio Ambiente , Variação Genética , Genética Populacional , Geografia , Repetições de Microssatélites , Polimorfismo Genético , Dinâmica Populacional
4.
Sci Rep ; 7(1): 1446, 2017 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-28469138

RESUMO

The establishment of a collaborative network of transcription factors (TFs) followed by decomposition and then construction of subnetworks is an effective way to obtain sets of collaborative TFs; each set controls a biological process or a complex trait. We previously developed eight gene association methods for genome-wide coexpression analysis between each TF and all other genomic genes and then constructing collaborative networks of TFs but only one algorithm, called Triple-Link Algorithm, for building collaborative subnetworks. In this study, we developed two more algorithms, Single Seed-Growing Algorithm (SSGA) and Multi-Seed Growing Algorithm (MSGA), for building collaborative subnetworks of TFs by identifying the fully-linked triple-node seeds from a decomposed collaborative network and then growing them into subnetworks with two different strategies. The subnetworks built from the three algorithms described above were comparatively appraised in terms of both functional cohesion and intra-subnetwork association strengths versus inter-subnetwork association strengths. We concluded that SSGA and MSGA, which performed more systemic comparisons and analyses of edge weights and network connectivity during subnetwork construction processes, yielded more functional and cohesive subnetworks than Triple-Link Algorithm. Together, these three algorithms provide alternate approaches for acquiring subnetworks of collaborative TFs. We also presented a framework to outline how to use these three algorithms to obtain collaborative TF sets governing biological processes or complex traits.


Assuntos
Algoritmos , Arabidopsis/genética , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Genoma de Planta , Fatores de Transcrição/genética , Arabidopsis/efeitos dos fármacos , Arabidopsis/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Folhas de Planta/metabolismo , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Caules de Planta/efeitos dos fármacos , Caules de Planta/genética , Caules de Planta/metabolismo , Salinidade , Tolerância ao Sal , Sais/farmacologia , Estresse Fisiológico , Fatores de Transcrição/metabolismo
5.
Sci Rep ; 6: 36286, 2016 11 08.
Artigo em Inglês | MEDLINE | ID: mdl-27824106

RESUMO

Although 5-HT has been implicated in cholestatic itch and antinociception, two common phenomena in patients with cholestatic disease, the roles of 5-HT receptor subtypes are unclear. Herein, we investigated the roles of 5-HT receptors in itch and antinociception associated with cholestasis, which was induced by common bile duct ligation (BDL) in rats. 5-HT-induced enhanced scratching and antinociception to mechanical and heat stimuli were demonstrated in BDL rats. 5-HT level in the skin and spinal cord was significantly increased in BDL rats. Quantitative RT-PCR analysis showed 5-HT1B, 5-HT1D, 5-HT2A, 5-HT3A, 5-HT5B, 5-HT6, and 5-HT7 were up-regulated in peripheral nervous system and 5-HT1A, 5-HT1F, 5-HT2B, and 5-HT3A were down-regulated in the spinal cord of BDL rats. Intradermal 5-HT2, 5-HT3, and 5-HT7 receptor agonists induced scratching in BDL rats, whereas 5-HT3 agonist did not induce scratching in sham rats. 5-HT1A, 5-HT2, 5-HT3, and 5-HT7 agonists or antagonists suppressed itch in BDL rats. 5-HT1A agonist attenuated, but 5-HT1A antagonist enhanced antinociception in BDL rats. 5-HT2 and 5-HT3 agonists or antagonists attenuated antinociception in BDL rats. Our data suggested peripheral and central 5-HT system dynamically participated in itch and antinociception under cholestasis condition and targeting 5-HT receptors may be an effective treatment for cholestatic itch.


Assuntos
Colestase/complicações , Dor/metabolismo , Prurido/metabolismo , Receptores de Serotonina/metabolismo , Animais , Colestase/etiologia , Colestase/metabolismo , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , Dor/tratamento farmacológico , Dor/etiologia , Dor/genética , Sistema Nervoso Periférico/metabolismo , Prurido/tratamento farmacológico , Prurido/etiologia , Prurido/genética , Ratos , Receptores de Serotonina/genética , Agonistas do Receptor 5-HT1 de Serotonina/administração & dosagem , Agonistas do Receptor 5-HT1 de Serotonina/uso terapêutico , Antagonistas do Receptor 5-HT1 de Serotonina/administração & dosagem , Antagonistas do Receptor 5-HT1 de Serotonina/uso terapêutico , Medula Espinal/metabolismo
6.
PLoS One ; 11(8): e0159458, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27541138

RESUMO

Moringa oleifera is a promising plant species for oil and forage, but its genetic improvement is limited. Our current breeding program in this species focuses on exploiting the functional genes associated with important agronomical traits. Here, we screened reliable reference genes for accurately quantifying the expression of target genes using the technique of real-time quantitative polymerase chain reaction (RT-qPCR) in M. oleifera. Eighteen candidate reference genes were selected from a transcriptome database, and their expression stabilities were examined in 90 samples collected from the pods in different developmental stages, various tissues, and the roots and leaves under different conditions (low or high temperature, sodium chloride (NaCl)- or polyethyleneglycol (PEG)- simulated water stress). Analyses with geNorm, NormFinder and BestKeeper algorithms revealed that the reliable reference genes differed across sample designs and that ribosomal protein L1 (RPL1) and acyl carrier protein 2 (ACP2) were the most suitable reference genes in all tested samples. The experiment results demonstrated the significance of using the properly validated reference genes and suggested the use of more than one reference gene to achieve reliable expression profiles. In addition, we applied three isotypes of the superoxide dismutase (SOD) gene that are associated with plant adaptation to abiotic stress to confirm the efficacy of the validated reference genes under NaCl and PEG water stresses. Our results provide a valuable reference for future studies on identifying important functional genes from their transcriptional expressions via RT-qPCR technique in M. oleifera.


Assuntos
Adaptação Fisiológica/genética , Perfilação da Expressão Gênica , Moringa oleifera/genética , Folhas de Planta/genética , Proteínas de Plantas/genética , Raízes de Plantas/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Estresse Fisiológico/genética , Moringa oleifera/crescimento & desenvolvimento , Folhas de Planta/crescimento & desenvolvimento , Raízes de Plantas/crescimento & desenvolvimento , Padrões de Referência , Transcriptoma
7.
Sci Rep ; 5: 16768, 2015 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-26602811

RESUMO

The contributions of gasotransmitters to itch sensation are largely unknown. In this study, we aimed to investigate the roles of hydrogen sulfide (H2S), a ubiquitous gasotransmitter, in itch signaling. We found that intradermal injection of H2S donors NaHS or Na2S, but not GYY4137 (a slow-releasing H2S donor), dose-dependently induced scratching behavior in a µ-opioid receptor-dependent and histamine-independent manner in mice. Interestingly, NaHS induced itch via unique mechanisms that involved capsaicin-insensitive A-fibers, but not TRPV1-expressing C-fibers that are traditionally considered for mediating itch, revealed by depletion of TRPV1-expressing C-fibers by systemic resiniferatoxin treatment. Moreover, local application of capsaizapine (TRPV1 blocker) or HC-030031 (TRPA1 blocker) had no effects on NaHS-evoked scratching. Strikingly, pharmacological blockade and silencing of Cav3.2 T-type calcium channel by mibefradil, ascorbic acid, zinc chloride or Cav3.2 siRNA dramatically decreased NaHS-evoked scratching. NaHS induced robust alloknesis (touch-evoked itch), which was inhibited by T-type calcium channels blocker mibefradil. Compound 48/80-induced itch was enhanced by an endogenous precursor of H2S (L-cysteine) but attenuated by inhibitors of H2S-producing enzymes cystathionine γ-lyase and cystathionine ß-synthase. These results indicated that H2S, as a novel nonhistaminergic itch mediator, may activates Cav3.2 T-type calcium channel, probably located at A-fibers, to induce scratching and alloknesis in mice.


Assuntos
Canais de Cálcio Tipo T/metabolismo , Prurido/induzido quimicamente , Prurido/fisiopatologia , Sulfetos/toxicidade , Acetanilidas/farmacologia , Animais , Comportamento Animal/efeitos dos fármacos , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio Tipo T/química , Canais de Cálcio Tipo T/genética , Capsaicina/análogos & derivados , Capsaicina/farmacologia , Capsaicina/uso terapêutico , Cistationina beta-Sintase/antagonistas & inibidores , Cistationina beta-Sintase/metabolismo , Cistationina gama-Liase/antagonistas & inibidores , Cistationina gama-Liase/metabolismo , Modelos Animais de Doenças , Diterpenos/farmacologia , Masculino , Mibefradil/farmacologia , Camundongos , Prurido/tratamento farmacológico , Purinas/farmacologia , Interferência de RNA , Receptores Opioides/metabolismo , Células Receptoras Sensoriais/metabolismo , Canal de Cátion TRPA1 , Canais de Cátion TRPV/antagonistas & inibidores , Canais de Cátion TRPV/genética , Canais de Cátion TRPV/metabolismo , Canais de Receptores Transientes de Potencial/antagonistas & inibidores , Canais de Receptores Transientes de Potencial/metabolismo
8.
Zhonghua Er Ke Za Zhi ; 41(2): 104-6, 2003 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-14759309

RESUMO

OBJECTIVE: Tissue factor (TF) is an important factor in extrinsic coagulation. Tissue factor pathway inhibitor (TFPI) is a negative regulator of coagulation mediated by TF. Studies on TF and TFPI focus mainly on adult objects, seldom have been done on newborns, especially on sick newborns. The aim of this study was to observe the changes of TF and TFPI in plasma of newborns with infection jaundice and to research the effect of jaundice and infection on the balance of TF and TFPI in newborns. METHODS: The content of TF and TFPI in plasma of 21 jaundiced newborns with infection and 8 jaundiced newborns without infection as control was determined quantitatively with the enzyme-linked immunosorbent assay (ELISA). RESULTS: The content of TFPI and TF in plasma of jaundiced newborn with infection was significantly higher than that of controls [TFPI (21.0 +/- 4.3) vs. (16.2 +/- 1.9) microg/L, P < 0.01; TF (177 +/- 79) vs. (51 +/- 24) ng/L, P < 0.01]. The ratio of TFPI/TF was significantly lower in newborn with infection jaundice than the controls (137 +/- 61 vs. 319 +/- 67, P < 0.01). The 21 jaundiced newborns with infection were divided into the severe hyperbilirubinemia group (serum bilirubin > or = 205.2 micromol/L, n = 10) and the mild hyperbilirubinemia group (serum bilirubin < 205.2 micromol/L, n = 11). There was no significant difference of TFPI level between the severe hyperbilirubinemia group and mild hyperbilirubinemia group (P > 0.05). The TF content in the severe hyperbilirubinemia group was higher than that in the mild hyperbilirubinemia group (216 +/- 79 vs.141 +/- 63, P < 0.01), while the ration of TFPI/TF was lower in the severe hyperbilirubinemia group than in the mild hyperbilirubinemia group (100 +/- 30 vs. 171 +/- 74, P < 0.01). CONCLUSION: Infection might induce imbalance between the coagulation inhibition and activation in newborns. Hyperbilirubinemia can aggravate the imbalance induced by the infection through increasing plasma TF level.


Assuntos
Infecções Bacterianas/sangue , Icterícia Neonatal/sangue , Lipoproteínas/sangue , Tromboplastina/análise , Infecções Bacterianas/complicações , Estudos de Casos e Controles , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Recém-Nascido , Icterícia Neonatal/etiologia , Masculino
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