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1.
Transl Pediatr ; 10(9): 2347-2354, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34733675

RESUMO

Background: There is no clear clinical conclusion on whether assisted reproductive technology (ART) increases maternal and child risk and affects infant birth and development. This study aimed to perform a multiple regression analysis of the perinatal maternal and infant conditions in an assisted reproductive singleton pregnancy and the physical development and complications of such infants at 6 months old. Methods: This study enrolled 145 singleton pregnant women who were admitted to Luohe Central Hospital between December 2017 and December 2019 to undergo in vitro fertilization and embryo transfer as a research group, and 160 singleton pregnant women who were naturally conceived at the same time and delivered at our hospital were selected as the control group. The relevant data of the patients were collected, and the perinatal conditions, neonatal complications, physical development and NBN score of infants aged 6 months were compared between the two groups. Multivariate logistic regression was used to analyze risk factors for perinatal complications in assisted reproduction singleton pregnancy. Results: The incidence of complications in the perinatal period in the study group was significantly higher than that in the control group (P<0.05). However, there was no significant difference in the incidence of complications such as very low birth weight, respiratory distress, neonatal hemolysis, hyperbilirubinemia, hypoglycemia, infectious pneumonia and neonatal asphyxia between the two groups (P>0.05). Likewise, at 6 months of age, the infants in the research group showed no significant difference to the control group in physical development indicators, including weight, head circumference, body mass index, height and NBN score (P>0.05). The analysis of the unconditional multivariate logistic regression model revealed hypertension during pregnancy, placental previa, premature membrane rupture, gestational age <32 weeks, and very low birth weight as risk factors that affect perinatal complications in patients with assisted reproductive singleton pregnancy (P<0.05). Conclusions: The incidence of complications in perinatal patients with assisted reproductive singleton pregnancy is higher than that of natural singleton pregnancy, but there is no significant difference in physical development, NBN score and complications of 6 months old infants.

2.
Ann Transl Med ; 9(18): 1451, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34734003

RESUMO

Background: MicroRNA (miRNA) plays an important role in hepatic stellate cell (HSCs) activation and liver fibrosis. The purpose of this study is to explore the effect of hypoxia on the differential expression of mRNAs and miRNAs in rat HSCs. Methods: HSC-T6 cells were treated with cobalt chloride (CoCl2), and the activity of HSC-T6 cells was measured by the CCK-8 assay. The mRNA expression levels of hypoxia inducible factor-1α (HIF-1α), collagen type I, transforming growth factor-ß1 (TGF-ß1), and Smad7 were measured by RT-qPCR. The protein expression levels of HIF-1α, Bax, Bcl-2, and caspase-3 were assayed by western blot. We used basal medium and 400 µmol/L CoCl2 medium to treat HSC-T6 cells for 48 h. Cells were harvested after 48 h to extract RNA. Transcriptome sequencing was performed to investigate differentially expressed miRNAs and mRNAs (fold change >2; P<0.05). Bioinformatics analysis was performed to predict the functions of differentially expressed miRNAs and mRNAs. Further, we used RT-qPCR to detect the expression of mRNAs and miRNAs to confirm the accuracy of sequencing. Results: With the increase of CoCl2 concentration, the activity of HSC-T6 cells decreased (P<0.05). The mRNA expression levels of HIF-1α, collagen I, TGF-ß1, and Smad7, and the protein expressions levels of HIF-1α, Bax, caspase-3, and the Bcl-2/Bax ratio were increased compared with the control group (P<0.05), while the expression of Bcl-2 decreased. A total of 54 miRNAs (20 upregulated and 34 downregulated) and 1,423 mRNAs (685 upregulated and 738 downregulated) were differentially expressed in the 400 µmol/L CoCl2 medium group compared to the control basal medium group. Further bioinformatics analysis demonstrated that the differentially expressed mRNAs and miRNAs were mainly enriched in the synthesis of extracellular matrix. In addition, we used RT-qPCR to detect the expression of mRNAs and miRNAs to confirm the accuracy of sequencing. Conclusions: Our results presented the profiles of mRNAs and miRNAs in hypoxia-induced HSC-T6 cells in rats, the signaling pathways, and co-expression networks. These findings may suggest novel insights for the early diagnosis and treatment of HSC activation and liver fibrosis.

3.
Cell Death Discov ; 7(1): 361, 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34802056

RESUMO

The current study tried to uncover the molecular mechanism of E3 ubiquitin ligase F-box and WD repeat domain-containing 7 (FBW7) in a heritable autoimmune disease, type I diabetes (T1D). After streptozotocin-induced T1D model establishment in non-obese diabetic (NOD) mouse, the protein expression of FBW7, enhancer of zeste homolog 2 (EZH2), and Zinc finger and BTB domain containing 16 (ZBTB16) was quantified. Next, splenocytes and pancreatic beta cells were isolated to measure the production of pro-inflammatory cytokines in splenocytes, as well as islet beta-cell apoptosis. Additionally, the stability of EZH2 induced by FBW7 was analyzed by cycloheximide chase assay. The binding affinity of FBW7 and EZH2 and the consequence of ubiquitination were monitored by co-immunoprecipitation assay. Last, a chromatin immunoprecipitation assay was employed to analyze the accumulation of EZH2 and H3K27me3 at the ZBTB16 promoter region. Our study demonstrated downregulated FBW7 and ZBTB16 and upregulated EZH2 in diabetic NOD mice. Overexpression of FBW7 in the NOD mice inhibited pro-inflammatory cytokine release in the splenocytes and the apoptosis of islets beta cells. FBW7 destabilized EZH2 and accelerated ubiquitin-dependent degradation. EZH2 and H3K27me3 downregulated the ZBTB16 expression by accumulating in the ZBTB16 promoter and methylation. FBW7 upregulates the expression of ZBTB16 by targeting histone methyltransferase EZH2 thus reducing the occurrence of T1D.

4.
Sci Rep ; 11(1): 21871, 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34750490

RESUMO

Derived from the most abundant natural polymer, cellulose nanocrystal materials have attracted attention in recent decades due to their chemical and mechanical properties. However, still unclear is the influence of different exposed facets of the cellulose nanocrystals on the physicochemical properties. Herein, we first designed cellulose II nanocrystals with different exposed facets, the hydroxymethyl conformations distribution, hydrogen bond (HB) analysis, as well as the relative structural stability of these models (including crystal facets {A, B, O} and Type-A models vary in size) are theoretically investigated. The results reveal that the HB network of terminal anhydroglucose depends on the adjacent chain's contact sites in nanocrystals exposed with different facets. Compared to nanocrystals exposed with inclined facet, these exposed with flat facet tend to be the most stable. Therefore, the strategy of tuning exposed crystal facets will guide the design of novel cellulose nanocrystals with various physicochemical properties.

5.
Artigo em Inglês | MEDLINE | ID: mdl-34762410

RESUMO

Currently, the exploration of wearable strain sensors that can work under subzero temperatures while simultaneously possessing anti-interference capability toward temperature is still a grand challenge. Herein, we present a low-temperature wearable strain sensor that is constructed via the incorporation of a Ag nanowires/graphene (Ag NWs/G) composite into the polydimethylsiloxane (PDMS) polymer. The Ag NWs/G/PDMS strain sensor exhibits promising flexibility at a very low temperature (-40 °C), outstanding fatigue resistance with low hysteresis energy, and near-zero temperature coefficient of resistance (TCR). The Ag NWs/G/PDMS strain sensor shows excellent sensing performance under subzero temperatures with a very high gauge factor of 9156 under a strain of >36%, accompanied by a noninterference characteristic to temperature (-40 to 20 °C). The Ag NWs/G/PDMS strain sensor also demonstrates the feasibility of monitoring various human movements such as finger bending, arm waving, wrist rotation, and knee bending under both room temperature and low-temperature conditions. This work initiates a new promising strategy to construct next-generation wearable strain sensors that can work stably and effectively under very low temperatures.

6.
Diabetes Metab Syndr Obes ; 14: 4497-4503, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34785919

RESUMO

Objective: This study aims to explore the factors influencing the renal glucose threshold (RTG) in patients with newly diagnosed type 2 diabetes mellitus (T2DM). Methods: A cross-sectional study was conducted on 1009 hospitalized patients with T2DM using stratified random sampling. Blood glucose was monitored using a dynamic blood glucose monitor to obtain the mean blood glucose (MBG), which is used to calculate the RTG. The factors influencing the RTG were then analyzed. Results: The mean RTG in patients with newly diagnosed T2DM was 203.58 ± 55.22 mg/dl. The correlation between the RTG and the various variables was analyzed, and the results demonstrated that the RTG was correlated with the patient's age (r = -0.14539, P = 0.0001); MBG (r = -0.35009, P = 0.0001); renal long neck (r = 0.16762, P = 0.0001); homeostatic model assessment for insulin resistance (r = -0.38322, P = 0.0001); homeostatic model assessment for beta-cell function (r = -0.22770, P = 0.0001); and the levels of glycated hemoglobin (HbA1c; r = 0.98994, P = 0.0001), blood urea nitrogen (r = -0.11093, P = 0.0004), creatinine (r = -0.26414, P = 0.0001), uric acid (r = -0.20149, P = 0.0001), total cholesterol (r = 0.13192, P = 0.0001), low-density lipoprotein (r = 0.12466, P = 0.0001), thyroid-stimulating hormone (r = -0.06346, P = 0.0460), beta-2 microglobulin (r = -0.08884, P = 0.0056), and 24-hour urine glucose (r = 0.32115, P = 0.0001). Multiple linear stepwise regression analysis revealed that the HbA1c, 24-hour urine glucose, estimated glomerular filtration rate (eGFR), D-dimer, and body mass index (BMI) should be included in the final model, and HbA1c had the greatest impact on the RTG followed in descending order by the 24-hour urine glucose, eGFR, D-dimer, and BMI (P < 0.05). Conclusion: The RTG increases in most patients with newly diagnosed diabetes. The risk factors for the RTG are HbA1c, 24-hour urine glucose, eGFR, D-dimer, and BMI.

7.
Front Bioeng Biotechnol ; 9: 773104, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34765597

RESUMO

Sophorolipids (SLs) are homologous microbial secondary metabolites produced by Starmerella bombicola and have been widely applied in many industrial fields. The biosynthesis of SLs is a highly aerobic process and is often limited by low dissolved oxygen (DO) levels. In this study, the Vitreoscilla hemoglobin (VHb) gene was transformed into S. bombicola O-13-1 by homologous recombination to alleviate oxygen limitation. VHb expression improved the intracellular oxygen utilization efficiency under either oxygen-rich or oxygen-limited conditions. In shake flask culture, the production of SLs was higher in the recombinant (VHb+) strain than in the wild-type (VHb-) strain, while the oxygen uptake rate of the recombinant (VHb+) strain was significantly lower than that of the wild-type (VHb-) strain. In a 5 L bioreactor, the production of SLs did not increase significantly, but the DO level in the fermentation broth of the VHb+ strain was 21.8% higher than that of VHb- strain under oxygen-rich conditions. Compared to wide-type strains (VHb-), VHb expression enhanced SLs production by 25.1% in the recombinants (VHb+) under oxygen-limited conditions. In addition, VHb expression raised the transcription levels of key genes involved in the electron transfer chain (NDH, SDH, COX), TCA cycle (CS, ICD, KDG1) and SL synthesis (CYP52M1 and UGTA1) in the recombinant (VHb+) strains. VHb expression in S. bombicola could enhance SLs biosynthesis and intracellular oxygen utilization efficiency by increasing ATP production and cellular respiration. Our findings highlight the potential use of VHb to improve the oxygen utilization efficiency of S. bombicola in the industrial-scale production of SLs using industrial and agricultural by-products like molasses and waste oil as fermentation feedstock.

8.
Front Psychiatry ; 12: 751411, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34744840

RESUMO

Background: This study investigates the mediating effect of rumination on the associations between depressive symptoms and insomnia. Methods: This is a cross-sectional study. Insomnia Severity Index (ISI), Ruminant Response Scale (RRS) and Beck Depression Inventory (BDI) were determined in 12,178 college students in Qinghai province by a questionnaire network platform. Results: The prevalence of insomnia was 38.6% in the participants. Insomnia symptoms [interquartile range: 6 (3, 9)], depressive symptoms [interquartile range: 5 (1, 9)], and rumination [interquartile range: 22 (20, 26)] were positively correlated (r = 0.25-0.46, p < 0.01). Mediation effect analysis showed that the depressive symptoms affected insomnia directly and indirectly. The direct effect and the indirect effect through rumination account for 92.4 and 7.6% of the total effect, respectively. Conclusion: The study shows that insomnia, depressive symptoms, and rumination are related constructs in college students in Qinghai province. It demonstrates the direct effects and the rumination-mediated indirect effects between depressive symptoms and insomnia; the direct effects seem to be dominant.

9.
Ann Palliat Med ; 10(9): 9572-9582, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34628883

RESUMO

BACKGROUND: The current focus is largely on whole course medical management of coronavirus disease-19 (COVID-19) with real-time polymerase chain reaction (RT-PCR) and radiological features, while the mild cases are usually missed. Thus, combination of multiple diagnostic methods is urgent to understand COVID-19 fully and to monitor the progression of COVID-19. METHODS: laboratory variables of 40 mild COVID-19 patients, 30 patients with community-acquired pneumonia (CAP) and 32 healthy individuals were analyzed by principal component analysis (PCA), Kruskal test, Procrustes test, the vegan package in R, CCA package and receiver operating characteristic to investigate the characteristics of the laboratory variables and their relationships in COVID-19. RESULTS: The correlations between the laboratory variables presented a variety of intricate linkages in the COVID-19 group compared with the healthy group and CAP patient group. The prediction probability of the combination of lymphocyte count (LY), eosinophil (EO) and platelets (PLT) was 0.847, 0.854 for the combination of lactate (LDH), creatine kinase isoenzyme (CK-MB), and C-reactive protein (CRP), 0.740 for the combination of EO, white blood cell count (WBC) and neutrophil count (NEUT) and 0.872 for the combination of CK-MB and P. CONCLUSIONS: The correlations between the laboratory variables in the COVID-19 group could be a unique characteristic showing promise as a method for COVID-19 prediction and monitoring progression of COVID-19 infection.


Assuntos
COVID-19 , Infecções Comunitárias Adquiridas , Pneumonia , Estudos de Coortes , Humanos , Pneumonia/diagnóstico , SARS-CoV-2
10.
Int J Mol Sci ; 22(20)2021 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-34681591

RESUMO

Phosgene (COCl2) was once used as a classic suffocation poison and currently plays an essential role in industrial production. Due to its high toxicity, the problem of poisoning caused by leakage during production, storage, and use cannot be ignored. Phosgene mainly acts on the lungs, causing long-lasting respiratory depression, refractory pulmonary edema, and other related lung injuries, which may cause acute respiratory distress syndrome or even death in severe cases. Due to the high mortality, poor prognosis, and frequent sequelae, targeted therapies for phosgene exposure are needed. However, there is currently no specific antidote for phosgene poisoning. This paper reviews the literature on the mechanism and treatment strategies to explore new ideas for the treatment of phosgene poisoning.

11.
J Appl Microbiol ; 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34623726

RESUMO

AIMS: This study aimed to separate the effects of chemical and microbial factors on the fermentation quality and bacterial community of ensiled Pennisetum giganteum. METHODS AND RESULTS: Fresh P. giganteum of two vegetative stages (stage I, GI ; stage II, GII ) was treated as follows: GI epiphytic microbiota + Î³-ray presterilized GI (MI CI ), GII epiphytic microbiota + Î³-ray presterilized GI (MII CI ), GI epiphytic microbiota + Î³-ray presterilized GII (MI CII ), and GII epiphytic microbiota + Î³-ray presterilized GII (MII CII ). Triplicates per treatment were sampled after 30 days of ensiling for chemical and microbial analyses and high-throughput sequencing. Silages made from CII (MI CII and MII CII ) had higher lactic acid concentration and the ratio of lactic to acetic acid, and lower pH and ammonia nitrogen concentration than silages produced by CI (MI CI and MII CI ). Species differential analyses showed that the changes of chemical composition rather than epiphytic microbiota significantly affected the relative abundance of Lactobacillus, Pediococcus and Pantoea in P. giganteum silages. CONCLUSION: These above results manifested that chemical composition was the main factor influencing the fermentation quality and bacterial community of P. giganteum silage in this study. SIGNIFICANCE AND IMPACT OF THE STUDY: The obtained results may, therefore, be the first record to provide an in-depth understanding of the relative contributions of chemical and microbial parameters on fermentation quality and bacterial community, which is of great importance for modulating silage fermentation and improving silage quality.

12.
J Appl Microbiol ; 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34662476

RESUMO

AIMS: To isolate and enrich lignocellulolytic microbial consortia from yak (Bos grunniens) rumen and evaluate their effects on the fermentation characteristics and enzymatic hydrolysis in rice straw silage. METHODS AND RESULTS: A novel microbial consortium M2 with high CMCase and xylanase activities was enriched and observed to be prone to use natural carbon sources. Its predominant genus was Enterococcus, and most carbohydrate-active enzyme (CAZyme) genes belonged to the glycosyl hydrolases class. The consortium M2 was introduced with or without combined lactic acid bacteria (XA) to rice straw silage for 60 days. Inoculating the consortium M2 notably decreased the structural carbohydrate contents and pH of rice straw silages. Treatment that combines consortium M2 and XA resulted in the highest levels of lactic acid and lignocellulose degradation. The consortium M2 alone or combined with XA significantly (p < 0.01) increased water-soluble carbohydrates (WSCs), mono- and disaccharides contents compared with the XA silage. Combined addition obviously improved the enzymatic conversion efficiency of rice straw silage with higher glucose and xylose yields (23.39 and 12.91 w/w% DM, respectively). CONCLUSIONS: Ensiling pretreatment with the microbial consortium M2 in sterile rice straw improved fermentation characteristics. The combined application of consortium M2 with XA had synergistic effects on promoting the degradation of structural carbohydrates and enzymatic hydrolysis. SIGNIFICANCE AND IMPACT OF THE STUDY: Rice straw is difficult to ensile because of its low WSC and high structural carbohydrate contents. The microbial consortium M2 identified herein exhibits great potential for degrading fibrous substrates, and their combination with XA provides a faster and more effective synergistic strategy for biorefinery of lignocellulosic biomass.

13.
J Mater Chem B ; 9(44): 9191-9203, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34698324

RESUMO

Traditional shape memory polymers (SMPs) could avoid large volume trauma during implantation; however, for bone repair, scaffolds with high porosity and biomineralization are essential to promote bone regeneration. A novel porous composite scaffold with high biomineralization activity was developed by sequential gas foaming and a freeze-drying method. The results showed that the cross-linked block structure of the polymer matrix presented excellent shape memory properties, and osteogenesis was promoted by citrate functionalized amorphous calcium phosphate (CCACP). CCACP improved the mechanical strength of the scaffold, and the synergistic effect of CCACP and PEG promotes hydrophilicity and further promoted cell adhesion. Bending experiments indicated that the shape-memory effect of the scaffolds could be varied by varying the CCACP content. In addition, hydroxyapatite deposition was sped up as CCACP accelerated the mineralization of the scaffolds. Moreover, the result of the CCK-8 assessment suggested that composite scaffolds exhibited high biocompatibility, and the cells extended out abundant filopodia to adhere onto the scaffolds. In rat bone defect models, the obtained scaffolds promoted new bone formation compared to the control group. The developed composite scaffolds show potential for minimally invasive bone repair application.

14.
World J Microbiol Biotechnol ; 37(12): 204, 2021 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-34677690

RESUMO

The objective of this study was to explore the microbiological factors that cause the difference in silage fermentation characteristics between grass and legume. Specifically, the effects of epiphytic microbiota from alfalfa, oat and Italian ryegrass on ensiling characteristics and microbial community of alfalfa were assessed. By γ-ray irradiation sterilization and microbiota transplantation technology, the sterile alfalfa was inoculated as follows: (i) aseptic water (STAL); (ii) epiphytic bacteria from alfalfa (ALAL); (iii) epiphytic bacteria from oat (ALOT); (iv) epiphytic bacteria from Italian ryegrass (ALIR). Alfalfa at the initial flowering stage was ensiled in laboratory-scale silos for 1, 3, 7, 14, 30 and 60 days. Compared with ALAL and ALIR, higher lactic acid contents and ratio of lactic acid to acetic acid, and lower acetic acid, propionic acid, ethanol and ammonia nitrogen contents were observed in ALOT after 60 days of fermentation. In each treated group, Lactobacillus was the most dominant genus after 60 days of ensiling. Relatively higher abundance of Weissella, Hafnia-Obesumbacterium, Enterobacteriaceae or hetero-fermentative Lactobacillus was found in ALAL and ALIR after 60 days. Co-occurrence network analysis proved Pediococcus and Lactococcus were pivotal in deciding the fermentation pattern of alfalfa silage. According to the 16S rRNA gene-predicted functional profiles, the metabolism of amino acids was inhibited by the epiphytic microbiota from oat. Overall, ALOT showed a homo-fermentative process, whereas ALAL and ALIR exhibited a hetero-fermentative pattern. Furthermore, the exogenous microorganisms inhibiting the metabolism of amino acids can be a good potential source to improve the silage quality of legume forage.

15.
Transl Psychiatry ; 11(1): 526, 2021 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-34645783

RESUMO

Brain function relies on efficient communications between distinct brain systems. The pathology of major depressive disorder (MDD) damages functional brain networks, resulting in cognitive impairment. Here, we reviewed the associations between brain functional connectome changes and MDD pathogenesis. We also highlighted the utility of brain functional connectome for differentiating MDD from other similar psychiatric disorders, predicting recurrence and suicide attempts in MDD, and evaluating treatment responses. Converging evidence has now linked aberrant brain functional network organization in MDD to the dysregulation of neurotransmitter signaling and neuroplasticity, providing insights into the neurobiological mechanisms of the disease and antidepressant efficacy. Widespread connectome dysfunctions in MDD patients include multiple, large-scale brain networks as well as local disturbances in brain circuits associated with negative and positive valence systems and cognitive functions. Although the clinical utility of the brain functional connectome remains to be realized, recent findings provide further promise that research in this area may lead to improved diagnosis, treatments, and clinical outcomes of MDD.


Assuntos
Conectoma , Transtorno Depressivo Maior , Encéfalo/diagnóstico por imagem , Humanos , Imageamento por Ressonância Magnética , Rede Nervosa/diagnóstico por imagem
16.
Risk Manag Healthc Policy ; 14: 4311-4319, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34703342

RESUMO

Objective: To investigate the cortisol level in peripheral blood in evaluating the severity and prognosis of patients with liver failure. Methods: A total of 211 inpatients with liver failure were enrolled in Beijing You 'an Hospital affiliated with Capital Medical University, from September 2018 to January 2020. They were divided into three groups according to the severity of liver failure: early-stage liver failure, medium-stage liver failure and end-stage liver failure. In all patients, peripheral venous blood was gathered to detect the level of peripheral cortisol and related biochemical indexes. Logistic regression was used to screen the risk factors affecting the prognosis of patients at 90 days. A receiver operating curve (ROC) was used to reflect the value of cortisol on the prognosis of patients at 90 days. A model for end-stage liver disease (MELD) was accessed. Results: Among these 211 patients with liver failure, aged from 19 to 70 years, the average level of cortisol was 351.26 ± 129.78 nmol/L for 101 cases with early-stage liver failure, 281.05 ± 81.31 nmol/L for 58 cases with medium-stage liver failure, and 189.21 ± 89.27 nmol/L for 52 cases with end-stage liver failure (P < 0.05). The level of cortisol in the survival group and the non-survival group was 339.26 ± 121.19 nmol/L and 192.71 ± 67.23 nmol/L, respectively (P < 0.05). The results of logistics regression analysis showed that the odds ratio (OR) and 95% confidence intervals (CI) of cortisol were 1.025 and 1.013-1.038 nmol/L (P < 0.05). MELD results showed that patients with higher MELD scores had lower cortisol content. Conclusion: The level of cortisol is correlated with the severity of liver failure and decreases with the aggravation of liver failure, suggesting that the level of cortisol could be used as one of the indicators to evaluate the prognosis in patients with liver failure at 90 days.

17.
Int Immunopharmacol ; 101(Pt A): 108182, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34601330

RESUMO

The aim of the present study was to explore the effect of hepatitis B virus on T lymphocyte and its subsets in different ALT states, and elucidate the immunological mechanism of ALT basing antiviral therapy for hepatitis B. 363 chronic hepatitis B patients were selected as the study subjects. According to ALT abnormalities, the patients were divided into three study groups. ALT normal group 131 cases, normal≦ ALT < 2 times of upper limit group 110 cases, ALT ≥ 2 times of upper limit group 122 cases. Entecavir was given to the ALT ≥ 2 times of upper limit group patients and followed up for 24 weeks. The hepatitis B antigen antibody parameters were measured by chemiluminescence immunoassay analyzer, the liver function parameters were measured by automatic biochemical analyzer, the hepatitis B virus load were measured by quantitative PCR analyzer, T lymphocytes were detected by flow cytometry, the level of IL-2, IFN-γ, IL-4 and IL-10 were detected by enzyme-linked immunosorbent assay. Detecting the influence of different hepatitis B viru loads in different groups on immunological indexes, and the virological and immunological indexes changes in before and after antiviral therapy patients. In the ALT normal group, different virus load hepatitis B virus had minor effect on T lymphocytes and their subsets (P > 0.05). In the ALT ≥ double upper limit of normal group. with the virus load increased, The total number of T lymphocytes, CD3+ CD4 + T lymphocytes decreased, (P < 0.05)CD3+ CD8 + T lymphocytes increased(P < 0.05). With the virus load increased the cytokines IL-2, IFN-γ which reflect the Th1 lymphocytes increased(P < 0.05), the cytokines IL-4、IL-10 which reflect the Th2 lymphocytes decreased(P < 0.05). Before and after 24 weeks of entecavir treatment, the patient's HBV-DNA decreased significantly(P < 0.05) and the body's immune function improved significantly. (P < 0.05)The influence of hepatitis B virus on immune function is different in different ALT states. Therefore, the scientific significance of ALT grouping in the hepatitis B treatment can be clarified from the immunological point.

18.
Arch Microbiol ; 203(10): 6007-6019, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34665278

RESUMO

This study was designed to evaluate the effects of epiphytic microbiota from Italian ryegrass (IRIR), Napier grass (IRNP) and Sudan grass (IRSD) on ensiling characteristics and microbial community of Italian ryegrass silage. Each treatment was prepared in triplicate and ensiled in plastic bag silos for 1, 3, 7, 14, 30 and 60 days. The γ-ray irradiation sterilization method, microbiota transplantation and next generation sequencing technology were used. Results indicated that significantly (P < 0.05) higher ratio of lactic acid to acetic acid, and lower acetic acid and ammonia nitrogen contents were observed in IRNP than IRIR and IRSD after 60 days of ensiling. Lactobacillus was the most predominant in each treatment at the late stage of fermentation. Lactococcus was eventually replaced by Lactobacillus in IRSD, whereas higher abundance of Lactococcus was continuously found in IRNP. Co-occurrence network analysis demonstrated Lactococcus was pivotal in determining the silage fermentation pattern of Italian ryegrass. According to the 16S rRNA gene-predicted functional profiles, the metabolism of amino acids was enhanced by the epiphytic microbiota from Italian ryegrass and Sudan grass, while the carbohydrate metabolism was accelerated by the epiphytic microbiota from Napier grass. Overall, IRNP had a homo-fermentative process, whereas IRIR and IRSD possessed a hetero-fermentative pattern. The Lactococcus and heterofermentative Lactobacillus were mainly responsible for this. It also confirmed that the exogenous microorganisms that promote the carbohydrate metabolism and inhibit the metabolism of amino acids could be a good potential source to improve the silage quality of temperate grass.


Assuntos
Microbiota , Sorghum , Fermentação , RNA Ribossômico 16S/genética , Silagem
19.
Bioresour Technol ; 344(Pt A): 126148, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34673188

RESUMO

Two consortia of lignocellulolytic microbes (CL and YL) were isolated from the rumen of ruminants. Their ability to facilitate the degradation of rice straw and enhance methane (CH4) production were evaluated, both individually and combined with lactic acid bacteria (LAB). After 30 days of degradation, rice straw powders (RSPs) were observed to change in physical structure and also displayed a significant reduction in lignocellulose content. Combined application of microbial consortia with LAB efficiently improved enzymatic hydrolysis of RSPs, increasing organic acid as well as mono- and disaccharide contents. Synergistic action between microbial consortia and LAB enhanced CH4 yield, and rice straw treated with YL + LAB had the highest CH4 production (357.53 mL CH4/g VS), more than fivefold of the control. The newly identified microbial consortia are capable of efficiently degrading lignocellulosic biomass. Functioning synergistically with LAB, they provide a feasible way biodegrade rice straw and enhance methane production from agricultural wastes.

20.
Nanoscale ; 13(39): 16680-16689, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34590639

RESUMO

Due to the complexity of surgery for large-area bone injuries, implanting a large volume of materials into the injury site remains a big challenge in orthopedics. To solve this difficulty, in this study, a series of biomimetic hydroxyapatite/shape-memory composite scaffolds were designed and synthesized with programmable pore structures, based on poly(ε-caprolactone) (PCL), polytetrahydrofuran (PTMG) and the osteoconductive hydroxyapatite (HA). The obtained scaffolds presented various pore structures, high connectivity, tunable mechanical properties, and excellent shape memory performance. Moreover, the mineralization activity of the developed scaffolds could enhance the formation of hydroxyapatite and they showed good biocompatibility in vitro. The in vivo experiments show that scaffolds could promote the formation of new bone in critical size cranial defects. The programmable porous scaffold biomaterials exhibited potential application promise in bone regeneration.


Assuntos
Biomimética , Tecidos Suporte , Regeneração Óssea , Durapatita , Poliésteres , Porosidade , Engenharia Tecidual
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