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1.
Artigo em Inglês | MEDLINE | ID: mdl-32388341

RESUMO

Litopenaeus vannamei (L. vannamei) is an essential aquaculture shrimp throughout the world, but its aquaculture industry is threatened by temperature fluctuation. In this study, our histological results indicated that the shrimp intestine has a self-repairing ability during temperature fluctuation; however the potential mechanisms were still unknown. Therefore, transcriptome profiles of the intestine were collected from shrimp at 28 °C (C28), 13 °C (T13) and 28 °C after their temperature rose back (R28) and were analyzed. A total of 2229 differentially expressed genes (DEGs) (986 up- and 1243 downregulated) were identified in the C28 group, and 1790 DEGs (933 up- and 857 downregulated) were identified in the R28 group when compared to their expression levels in the T13 group. According to the functional annotation using KEGG, we found that the immune system was the most enriched section of organismal systems and that the shrimp can mobilize the body's immune response to regulate organism homeostasis during temperature fluctuation, although cold stress decreased the immunity. Additionally, metabolic inhibition is a strategy to cope with cold stress, and the regulation of lipid metabolism was especially important for shrimp during temperature fluctuation. Remarkably, the Hippo signaling pathway might help the repair of intestinal structure. Our research provides the first histological analysis and transcriptome profiling for the L. vannamei intestine during the temperature fluctuation stage. These results enrich our understanding of the mechanism of intestinal self-repair and homeostasis and could provide guidance for shrimp farming.

2.
Brief Bioinform ; 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32363380

RESUMO

Competitive endogenous RNA (ceRNA) represents a novel layer of gene regulation that controls both physiological and pathological processes. However, there is still lack of computational tools for quickly identifying ceRNA regulation. To address this problem, we presented an R-package, CeRNASeek, which allows identifying and analyzing ceRNA-ceRNA interactions by integration of multiple-omics data. CeRNASeek integrates six widely used computational methods to identify ceRNA-ceRNA interactions, including two global and four context-specific ceRNA regulation prediction methods. In addition, it provides several downstream analyses for predicted ceRNA-ceRNA pairs, including regulatory network analysis, functional annotation and survival analysis. With examples of cancer-related ceRNA prioritization and cancer subtyping, we demonstrate that CeRNASeek is a valuable tool for investigating the function of ceRNAs in complex diseases. In summary, CeRNASeek provides a comprehensive and efficient tool for identifying and analysis of ceRNA regulation. The package is available on the Comprehensive R Archive Network (CRAN) at https://CRAN.R-project.org/package=CeRNASeek.

3.
Horm Metab Res ; 2020 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-32375182

RESUMO

Although glucagon-like peptide-1 (GLP-1) analogue has been reported to suppress oxidative stress in non-alcoholic fatty liver disease (NAFLD), an effective therapeutic agent for NAFLD is currently unavailable. Therefore, in this study, we aimed to investigate the protective effects of the GLP-1 analogue liraglutide against lipotoxicity-induced oxidative stress in HepG2 cells and to elucidate the underlying mechanisms. HepG2 cells were cultured for 48 hours and treated with a free fatty acid (FFA) mixture: FFA mixture and liraglutide or FFA mixture, liraglutide, and exendin (9-39). Lipid accumulation was examined by oil red O staining. Oxidative stress was assessed by measuring the levels of intracellular reactive oxygen species using 2',7'-dichlorofluorescein diacetate and thiobarbituric acid-reactive substances, whereas antioxidant capacity was assessed by measuring the activity of superoxide dismutase and catalase. Expression of the nuclear factor erythroid-2-related factor 2 (NRF2) gene and the genes encoding antioxidant enzymes was analyzed using quantitative RT-PCR. Cellular and nuclear NRF2 expression levels were assessed using immunofluorescence cell staining and western blotting. Liraglutide treatment reduced high fat-induced lipid formation and the levels of oxidative stress markers and increased antioxidant enzyme activity in HepG2 cells. Liraglutide treatment increased the mRNA expression of NRF2 target genes, induced NRF2 nuclear translocation, and increased nuclear NRF2 levels without altering NRF2 mRNA expression. Collectively, these results indicate that liraglutide exhibits a protective effect against lipotoxicity-induced oxidative stress, possibly via modulation of NRF2 and expression of antioxidant enzymes in liver cells.

4.
Fish Shellfish Immunol ; 103: 103-110, 2020 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-32348886

RESUMO

Temperature is a significant environmental factor contributing to the success of aquaculture. To investigate the immune defense response during temperature fluctuation, Litopenaeus vannamei (L. vannamei) was treated under conditions of gradual cooling from an acclimation temperature of 28 °C (C group) to 13 °C (T group) in 2 days with a cooling rate of 7.5 °C/d and then rewarmed to 28 °C (R group) using the same rate. Relative expression of immune defense system-related genes and intestinal microbial composition in L. vannamei were investigated. The results showed that with a decrease in temperature, the expression of TLR, IMD, proPO and Casp3 in intestine was significantly decreased, while the expression of Muc-3A, Muc-5AC, Muc-17, IAP, p53, HSP70, MT and Fer was significantly increased after cooling. After temperature recovery, gene expression generally showed a trend of recovering to the normal level (C). Intestinal microbial analysis showed that, compared with the C group, the Chao and Ace indexes, the relative abundance of microflora from the Phylum Bacteroidetes, Class Alphaproteobacteria, and Class Bacteroidia, significantly decreased in the R group. The results revealed that cold-stress may decrease microbial community richness, alter the bacterial community in general and reduce shrimp immunity to pathogens and antibacterial activity. As a result, during temperature fluctuation shrimp may mobilize the immune defense system through upregulating the expression of Muc genes, anti-apoptosis related genes, and antioxidant related genes in order to maintain organism homeostasis as well as to repair damaged intestinal tissue.

5.
J Am Chem Soc ; 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32159954

RESUMO

Organic-inorganic ABX3 (A, B = cations, X = anion) hybrids with perovskite structure have recently attracted tremendous interest due to their structural tunability and rich functional properties, such as ferroelectricity. However, ABX3 hybrid ferroelectrics with other structures have rarely been reported. Here, we successfully designed an ABX3 hybrid ferroelectric [(CH3)3NCH2F]ZnCl3 with a spontaneous polarization of 4.8 µC/cm2 by the molecular modification of [(CH3)4N]ZnCl3 through hydrogen/halogen substitution. It is the first zinc halide ABX3 ferroelectric, which contains one-dimensional [ZnCl3]-n chains of corner-sharing ZnCl4 tetrahedra, distinct from the anionic framework of corner-sharing or face-sharing BX6 octahedra in the ABX3 perovskites. From zero dimension to one dimension, the high symmetry of ZnCl4 tetrahedra is broken, and all of them align along one direction to form a polar [ZnCl3]-n chain, beneficial to the generation of ferroelectricity. This finding provides an efficient polar anionic framework for enriching the family of hybrid ferroelectrics by assembling with various cations and should inspire further exploration of new classes of organic-inorganic ABX3 ferroelectrics.

6.
Cancer Res ; 80(10): 2067-2071, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32193291

RESUMO

Long noncoding RNAs (lncRNA) play important roles in maintaining morphology and function of tissues, and their regulatory effectiveness is closely associated with spatial expression. To provide a comprehensive spatial atlas of expression for lncRNA, we propose LncSpA (http://bio-bigdata.hrbmu.edu.cn/LncSpA) to explore tissue-elevated (TE) lncRNA across human normal and adult and pediatric cancer tissues. In total, 71,131 and 12,007 TE lncRNAs and 634 clinical-related TE lncRNAs were identified across 38 normal and 33 adult cancer tissues. Moreover, 4,688 TE and 413 clinical-related lncRNAs were identified in pediatric cancer. By quick searching or query options, users can obtain eight major types of detailed information for lncRNA via various visualization techniques, including qualitative and quantitative spatial expression in different resources, coexpressed mRNAs, predicted function, known disease association, and the potential to serve as diagnostic or prognostic markers. LncSpA will be a valuable resource to understand lncRNA functions across tissues and cancers, leading to enhanced therapeutic strategies in precision oncology. SIGNIFICANCE: LncSpA is a new interactive resource that provides the spatial expression pattern of lncRNA across thousands of normal and cancer samples representing major tissue types.

7.
J Environ Sci (China) ; 91: 177-188, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32172966

RESUMO

Excessive nitrate (NO3-) is among the most problematic surface water and groundwater pollutants. In this study, a type of magnetic cationic hydrogel (MCH) is employed for NO3- adsorption and well characterized herein. Its adsorption capacity is considerably pH-dependent and achieves the optimal adsorption (maximum NO3--adsorption capacity is 95.88 ± 1.24 mg/g) when the pH level is 5.2-8.8. The fitting result using the homogeneous surface diffusion model indicates that the surface/film diffusion controls the adsorption rate, and NO3- approaches the center of MCH particles within 30 min. The diffusion coefficient (Ds) and external mass transfer coefficient (kF) in the liquid phase are 1.15 × 10-6 cm2/min and 4.5 × 10-6 cm/min, respectively. The MCH is employed to treat surface water that contains 10 mg/L of NO3-, and it is found that the optimal magnetic separation time is 1.6 min. The high-efficiency mass transfer and magnetic separation of MCH during the adsorption-regeneration process favors its application in surface water treatment. Furthermore, the study of the mechanism involved reveals that both -N+(CH3)3 groups and NO3- are convoluted in adsorption via electrostatic interactions. It is further found that ion exchange between NO3- and chlorine occurs.

8.
Int J Biol Macromol ; 153: 912-920, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32169453

RESUMO

The influence of physicochemical properties of carrier oils on nanoemulsion stability and the bioaccessibility of lycopene were studied. Lycopene-loaded nanoemulsions were prepared by using sesame oil, linseed oil or walnut oil as the oil phase and lactoferrin as the emulsifier. The stability was investigated by particle size, zeta potential, pH sensitivity, thermal stability and lycopene retention. Results showed that the stability was positively correlated with oil density but negatively related to oil viscosity and unsaturation degree; the lycopene nanoemulsion prepared by sesame oil exhibited greater stability and a slower degradation rate of lycopene compared to the other nanoemulsions. In addition, the lycopene retention in sesame oil-nanoemulsions was significantly higher during the first three weeks of storage. The bioaccessibility of lycopene, as measured by a simulated gastrointestinal model, was greatly improved in the nanoemulsion system. The lycopene bioaccessibility was around 25% in sesame oil- and linseed oil-nanoemulsions, and 18% in walnut oil-nanoemulsions, showing a similar trend with their stability. This information may facilitate the design of more efficacious lycopene-fortified delivery systems.

9.
Int J Geriatr Psychiatry ; 35(5): 572-580, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32011785

RESUMO

OBJECTIVE: Spontaneous intracerebral hemorrhage (sICH) is a devastating disease that can lead to poststroke depression (PSD) and greatest impact on the quality of life (QOL) of patients. Mindfulness meditation was viewed as one of the effective ways to reduce PSD in patients with cancer. The present study tried to investigate whether mindfulness meditation has potential benefits in PSD and QOL for sICH patients in China. METHODS: Two hundred and two patients in West China Hospital, Sichuan University, enrolled from January 2017 to December 2018 were included in a randomized controlled trial. After removing missing values, there were 67 in control group and 67 in intervention group. Patients in intervention group received 2-month mindfulness-based cognitive therapy, and patients in control group received stress management education (ie, an active control). RESULTS: The results suggested that the significant differences of depression, trait mindfulness, social well-being, emotional well-being, and total score of QOL were found in intervention group from time 1 to time 2. Physical well-being and the score of NIH stroke scale experienced significant changes in both control group and intervention group over time. CONCLUSIONS: Mindfulness-based intervention has positive effects on sICH patients' depression, social well-being, and emotional well-being. However, the change of trait mindfulness over time could not explain these positive effects. Future studies could explore the mechanism of mindfulness-based intervention on sICH patients' depression and QOL and clarify the boundaries of the positive effects of mindfulness-based intervention.

10.
Artigo em Inglês | MEDLINE | ID: mdl-32092861

RESUMO

The machine comprehension research of clinical medicine has great potential value in practical application, but it has not received sufficient attention and many existing models are very time consuming for the cloze-style machine reading comprehension. In this paper, we study the cloze-style machine reading comprehension in the clinical medical field and propose a Gated Dilated Convolution with Attention (GDCA) model, which consists of a gated dilated convolution module and an attention mechanism. Our model has high parallelism and is capable of capturing long-distance dependencies. On the CliCR data set, our model surpasses the present best model on several metrics and obtains state-of-the-art result, and the training speed is 8 times faster than that of the best model.

11.
Nat Commun ; 11(1): 1000, 2020 02 21.
Artigo em Inglês | MEDLINE | ID: mdl-32081859

RESUMO

Long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression and they play fundamental roles in immune regulation. Here we introduce an integrated algorithm, ImmLnc, for identifying lncRNA regulators of immune-related pathways. We comprehensively chart the landscape of lncRNA regulation in the immunome across 33 cancer types and show that cancers with similar tissue origin are likely to share lncRNA immune regulators. Moreover, the immune-related lncRNAs are likely to show expression perturbation in cancer and are significantly correlated with immune cell infiltration. ImmLnc can help prioritize cancer-related lncRNAs and further identify three molecular subtypes (proliferative, intermediate, and immunological) of non-small cell lung cancer. These subtypes are characterized by differences in mutation burden, immune cell infiltration, expression of immunomodulatory genes, response to chemotherapy, and prognosis. In summary, the ImmLnc pipeline and the resulting data serve as a valuable resource for understanding lncRNA function and to advance identification of immunotherapy targets.


Assuntos
Biomarcadores Tumorais/genética , Biomarcadores Tumorais/imunologia , Neoplasias/genética , Neoplasias/imunologia , Oncogenes/imunologia , RNA Longo não Codificante/genética , RNA Longo não Codificante/imunologia , Algoritmos , Carcinoma Pulmonar de Células não Pequenas/classificação , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/imunologia , Bases de Dados Genéticas , Humanos , Neoplasias Pulmonares/classificação , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/imunologia , Neoplasias/classificação
12.
J Environ Sci (China) ; 89: 264-276, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31892398

RESUMO

A novel polycyclodextrin-modified magnetic cationic hydrogel (PCD-MCH) was developed and its performance, kinetics and mechanism for the removal of reactive brilliant red X-3B (X-3B) were studied. The results showed that the zeta-potential of PCD-MCH was 32.8 to 16.7mV at pH3.0-10.5. The maximum X-3B adsorption capacity of PCD-MCH was 2792.3mg/g. The adsorption kinetics could be well-described by the Weber-Morris model and the homogeneous surface diffusion model (HSDM). Diffusion stages corresponding to surface or film diffusion, intra-particle or wide mesopore diffusion, and narrow mesopore/micropore diffusion occurred at 0-120, 120-480 and 480-1200min, respectively. The latter two diffusion stages were rate-controlling for X-3B adsorption kinetics. At the initial X-3B concentration of 600mg/L, the diffusion coefficient (Ds) and external mass transfer coefficient in the liquid phase (kF) were 3×10-11cm2/min and 4.68×10-6cm/min, respectively. X-3B approaching the center of PCD-MCH particles could be observed at 360min. At the end of the third diffusion stage, the Cp at q/qe=0 was 45.20mg/L, which was close to the homogeneous Cp value of 46mg/L along the radius of PCD-MCH particles. At pH3.0-10.0, PCD-MCH showed stable X-3B adsorption capacities. After five regeneration-reuse cycles, the residual adsorption capacity of regenerated PCD-MCH was higher than 892.7mg/g. The corresponding adsorption mechanism was identified as involving electrostatic interactions, cyclodextrin cavities and hydrogen bonds, of which cyclodextrin cavities showed prominent capture performance towards dye molecules through the formation of inclusion complexes.


Assuntos
Corantes/química , Hidrogéis/química , Poluentes Químicos da Água , Purificação da Água/métodos , Adsorção , Cátions , Ciclodextrinas/química , Concentração de Íons de Hidrogênio , Cinética , Fenômenos Magnéticos
13.
Arch Microbiol ; 202(4): 843-857, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31894392

RESUMO

The bacterial community in mammalian gastrointestinal tract is abundant and complex. To date, little is known about the gut microbiota of wild boar. This study aimed to investigate the fecal bacterial diversity of wild boar and compare with commercial pig and domestic native pig. The diet composition showed that the diets of wild boar, commercial pig and domestic native pig were different from each other. More than 1,760,000 quality-filtered sequences were obtained, and the results revealed distinct compositions and diversity of fecal microbiota in three groups. PCoA and NMDS analyses showed that fecal bacterial communities of wild boar, commercial pig and domestic native pig formed distinctly different clusters. Although the three groups shared a large size of OTUs comprising a core microbiota community, a strong distinction existed at family and genus levels. Ruminococcaceae, Prevotellaceae and Christensenellaceae were more abundant in the feces of wild boar than in domestic native pig and commercial pig. At the genus level, the proportion of unidentified Christensenellaceae was remarkably higher in wild boar group, while commercial pig and domestic native pig group had a higher abundance of Streptococcus and Lactobacillus. Tax4Fun predictions of metagenome function showed statistically significant differences in the functions of fecal microbiota in three groups. There were more bacteria genes with amino acid metabolism, cell growth and death, cell motility, energy metabolism, immune system and environmental adaptation observed in wild boar feces, while commercial pig feces contained more bacteria genes with carbohydrate metabolism, drug resistance, aging, infectious diseases, lipid metabolism, endocrine and metabolic diseases. These results indicated that the fecal microbial ecosystem of the wild boar is significantly different from that of domestic native pig and commercial pig, suggesting that diet is an important factor leading to differences in bacterial abundance and diversity in feces.


Assuntos
Bactérias/classificação , Bactérias/genética , Biodiversidade , Fezes/microbiologia , Microbioma Gastrointestinal , RNA Ribossômico 16S/genética , Sus scrofa/microbiologia , Animais , Dieta , Trato Gastrointestinal/microbiologia , Genes Bacterianos/genética , Metagenoma
14.
Artigo em Inglês | MEDLINE | ID: mdl-31794884

RESUMO

Litopenaeus vannamei (L. vannamei) is one of the most important aquaculture shrimps in the world and its survival, growth, and distribution was seriously challenged by cold stress. To investigate the response of L. vannamei under acute cold stress, mRNA and microRNA (miRNA) profiles of the hepatopancreas which was collected from the control group (28 °C) and the treatment group (13 °C) were analyzed. The results showed that 1266 differentially expressed genes (DEGs) (502 up- and 764 down-regulated) and 60 differentially expressed miRNAs (34 up- and 26 down-regulated) were identified under acute cold stress respectively. According to the functional annotation, catalytic activity was the most significant term in the GO category of molecular function, and protein processing in endoplasmic reticulum (ER) was the most enriched KEGG pathway for mRNAs profile. For miRNAs profile, functional annotation of the target genes of the differentially expressed miRNAs indicated that oxidoreductase activity was the most significant term in the GO category of molecular function, and glucagon signaling pathway was the most enriched KEGG pathway. The results revealed that ER involved in the acute cold stress response and acute cold stress may induce the unfolded protein response of L. vannamei.

16.
Analyst ; 145(2): 385-392, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31844853

RESUMO

Patient survival remains poor even after diagnosis in lung cancer cases, and the molecular events resulting from lung cancer progression remain unclear. Raman spectroscopy could be used to noninvasively and accurately reveal the biochemical properties of biological tissues on the basis of their pathological status. This study aimed at probing biomolecular changes in lung cancer, using Raman spectroscopy as a potential diagnostic tool. Herein, biochemical alterations were evident in the Raman spectra (region of 600-1800 cm-1) in normal and cancerous lung tissues. The levels of saturated and unsaturated lipids and the protein-to-lipid, nucleic acid-to-lipid, and protein-to-nucleic acid ratios were significantly altered among malignant tissues compared to normal lung tissues. These biochemical alterations in tissues during neoplastic transformation have profound implications in not only the biochemical landscape of lung cancer progression but also cytopathological classification. Based on this spectroscopic approach, classification methods including k-nearest neighbour (kNN) and support vector machine (SVM) were successfully applied to cytopathologically diagnose lung cancer with an accuracy approaching 99%. The present results indicate that Raman spectroscopy is an excellent tool to biochemically interrogate and diagnose lung cancer.

17.
Genome Biol ; 20(1): 274, 2019 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-31842925

RESUMO

The alignment of long-read RNA sequencing reads is non-trivial due to high sequencing errors and complicated gene structures. We propose deSALT, a tailored two-pass alignment approach, which constructs graph-based alignment skeletons to infer exons and uses them to generate spliced reference sequences to produce refined alignments. deSALT addresses several difficult technical issues, such as small exons and sequencing errors, which break through bottlenecks of long RNA-seq read alignment. Benchmarks demonstrate that deSALT has a greater ability to produce accurate and homogeneous full-length alignments. deSALT is available at: https://github.com/hitbc/deSALT.


Assuntos
Alinhamento de Sequência/métodos , Animais , Humanos , Software
18.
Artigo em Inglês | MEDLINE | ID: mdl-31713833

RESUMO

White-tip nematode, Aphelenchoides besseyi is a kind of widely distributed migratory parasitic nematode that can infect plant shoots. Transcriptome sequencing of plant parasitic nematodes and their host plants is helpful for understanding their interaction relationship. This study first reported expression patterns of defense-related genes in rice, and rice transcriptomes at different periods after infection with A. besseyi. The result showed that the defense response pathways of rice changed obviously in the early stage of A. besseyi infection, including upregulated salicylic acid and jasmonate pathways and a downregulated ethylene pathway. Transcriptome analysis results suggested that A. besseyi infection was associated with the downregulation of multiple genes related to photosynthesis with possible suppression of the photosynthetic activity. It suggested that the photosynthesis system of rice could be suppressed by infections of migratory nematodes, including A. besseyi and Hirschmanniella oryzae, but was stimulated by that of a sedentary nematode, Meloidogyne graminicola, by comparing our study with the reported transcriptome. OS09G0417800 (OsWRKY62) might play an important role in the interaction of migratory nematodes and rice. It also indicated that the infection strategy of both A. besseyi and the reported migratory nematode H. oryzae was similar to that of the fungal pathogen Magnaporthe grisea. These results provided an interesting starting point to elucidate the mechanism of the interaction between rice and A. besseyi, as well as the host and migratory plant nematodes.

19.
PeerJ ; 7: e7957, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31720106

RESUMO

Aims: We sought to explore the role of the SLC6A3 rs393795 allelic variant in cerebral spontaneous activity and clinical features in Parkinson's disease (PD) via imaging genetic approach. Methods: Our study recruited 50 PD and 45 healthy control (HC) participants to provide clinical, genetic, and resting state functional magnetic resonance imaging (rs-fMRI) data. All subjects were separated into 16 PD-AA, 34 PD-CA/CC, 14 HC-AA, and 31 HC-CA/CC four subgroups according to SLC6A3 rs393795 genotyping. Afterwards, main effects and interactions of groups (PD versus HC) and genotypes (AA versus CA/CC) on cerebral function reflected by regional homogeneity (ReHo) were explored using two-way analysis of covariance (ANCOVA) after controlling age and gender. Finally, Spearman' s correlations were employed to investigate the relationships between significantly interactive brain regions and clinical manifestations in PD subgroups. Results: Compared with HC subjects, PD patients exhibited increased ReHo signals in left middle temporal gyrus and decreased ReHo signals in left pallidum. Compared with CA/CC carriers, AA genotype individuals showed abnormal increased ReHo signals in right inferior frontal gyrus (IFG) and supplementary motor area (SMA). Moreover, significant interactions (affected by both disease factor and allelic variation) were detected in right inferior temporal gyrus (ITG). Furthermore, aberrant increased ReHo signals in right ITG were observed in PD-AA in comparison with PD-CA/CC. Notably, ReHo values in right ITG were negatively associated with Tinetti Mobility Test (TMT) gait subscale scores and positively related to Freezing of Gait Questionnaire (FOG-Q) scores in PD-AA subgroup. Conclusions: Our findings suggested that SLC6A3 rs393795 allelic variation might have a trend to aggravate the severity of gait disorders in PD patients by altering right SMA and IFG function, and ultimately result in compensatory activation of right ITG. It could provide us with a new perspective for exploring deeply genetic mechanisms of gait disturbances in PD.

20.
J Gastrointest Oncol ; 10(5): 847-858, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31602322

RESUMO

Background: Ulcerative colitis (UC) is a chronic, relapsing and non-specific inflammatory disease, involving various genes and pathways in their pathogenesis. Increasing evidences have showed that microRNAs (miRNAs) act as key post-transcriptional regulators of gene expression in UC. This current study aimed to identify key miRNAs, potential target genes, and relevant pathways involved in UC to uncover their underlying molecular mechanisms by using bioinformatics analysis. Methods: The mRNA and miRNA expression profiles were retrieved and downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) and miRNAs (DEMIs) were obtained by using the R software package. Results: A total of 79 DEGs and 47 DEMIs were obtained. And a panel of miRNAs and their target mRNAs were identified. It showed that miR-1231 may be a key regulator for DUOX2 and TFF1. CCL11 may be potentially targeted by miR-625. MMP1 may play vital roles in the development of UC by regulating the miR-1228/PPAR signaling pathway. In addition, we validated the most significantly up/down-expressed miRNAs (miR-92b, miR-625) and two of their corresponding target mRNAs (AQP8 and TAGAP, CCL11 and CHI3L1) in colon tissues of UC models preliminarily. The results were consistent with the microarray analysis. Conclusions: These findings may provide new insights into representing key mechanisms associated with the development of UC.

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