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1.
Sci Rep ; 11(1): 16210, 2021 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-34376708

RESUMO

With the increased incidence and recognition, ulcerative colitis (UC) has become a global public health problem in the world. Although many immunosuppressant and biological drugs have been used for UC treatment, the cure rate is still very low. It is necessary to find some safe and long-term used medicine for UC cure. Recently, the Chinese traditional herb Danshen has been investigated in the treatment of UC. However, it is a limitation of Danshen that many of the active components in Danshen are not easily absorbed by the human body. Probiotics could convert macromolecules into smaller molecules to facilitate absorption. Thus, Lactobacillus rhamnosus (F-B4-1) and Bacillus subtillis Natto (F-A7-1) were screened to ferment Danshen in this study. The fermented Danshen products were gavaged in the dextran sulfate sodium (DSS)-induced UC model mice. Danshen had better results to attenuate symptoms of DSS-induced UC after fermented with F-B4-1 and F-A7-1. Loss of body weight and disease activity index (DAI) were reduced. The abnormally short colon lengths and colonic damage were recovered. And fermented Danshen had the better inhibitory effect than Danshen itself on pro-inflammatory cytokine expression during DSS-induced UC. The results indicated that compared with Danshen, fermented Danshen relieved DSS-induced UC in mice more effectively. Danshen fermented by probiotics might be an effective treatment to UC in clinic stage in the future.

2.
Oncol Rep ; 46(3)2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34278501

RESUMO

Renal cell carcinoma (RCC) is a major healthcare burden globally. Tumor­derived extracellular vesicles (EVs) contribute to the formation of a pro­metastatic microenvironment. In the present study, we explored the role and mechanism of RCC cell 786­O­derived EVs (786­O­EVs) in RCC. First, 786­O­EVs were extracted and identified, and EV internalization of RCC cells was observed. RCC cell malignant behaviors and long noncoding RNA (lncRNA) metastasis­associated lung adenocarcinoma transcript 1 (MALAT1) expression patterns were detected before and after 786­O­EV treatment. MALAT1 was intervened to evaluate RCC cell behaviors. The downstream mechanism involving MALAT1 was predicted. In addition, the relationship among MALAT1, transcription factor CP2 like 1 (TFCP2L1) and ETS proto­oncogene 1, transcription factor (ETS1) was analyzed. TFCP2L1 expression patterns were measured after 786­O­EV exposure. Tumor xenograft formation assay and lung metastasis model were adopted to verify the role of 786­O­EVs in vivo in RCC. It was found that 786­O­EVs could be internalized by RCC cells. 786­O­EVs promoted RCC cell malignant behaviors, accompanied by elevated MALAT1 expression levels. The 786­O­EVs with MALAT1 knockdown attenuated the promotive effect of sole 786­O­EVs on RCC cells. MALAT1 located ETS1 in the TFCP2L1 promoter and negatively regulated TFCP2L1, and ETS1 protein could specifically bind to MALAT1. 786­O­EVs enhanced the binding of ETS1 and the TFCP2L1 promoter and decreased TFCP2L1 expression. In vivo, 786­O­EVs promoted tumor growth and RCC lung metastasis, which was suppressed following inhibition of MALAT1. Our findings indicated that 786­O­EVs promoted RCC invasion and metastasis by transporting MALAT1 to promote the binding of transcription factor ETS1 and TFCP2L1 promoter.

3.
Int J Biol Macromol ; 183: 119-131, 2021 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-33905802

RESUMO

Hedyotis diffusa polysaccharides, as the main component and an important bioactive substance of Hedyotis diffusa, are effective immunomodulators with various pharmacological activities, including antitumour, anti-inflammatory, antioxidant, anti-fatigue and immunity-enhancing activities. The total polysaccharides extracted from Hedyotis diffusa and Scutellaria barbata have great effects in treating liver cancer, gastric cancer, rectal cancer, glioma and nasopharyngeal carcinoma. Moreover, different materials and extraction methods result in differences in the structure and bioactivity of Hedyotis diffusa polysaccharides. Therefore, this paper summarizes the isolation, purification, structural characteristics, pharmacological activities, and combined action of Hedyotis diffusa polysaccharides to provide a reference for further study.


Assuntos
Hedyotis/química , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Humanos , Fatores Imunológicos/química , Fatores Imunológicos/isolamento & purificação , Fatores Imunológicos/farmacologia , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Relação Estrutura-Atividade
5.
Immunity ; 53(4): 805-823.e15, 2020 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-33053330

RESUMO

The activating receptor CD226 is expressed on lymphocytes, monocytes, and platelets and promotes anti-tumor immunity in pre-clinical models. Here, we examined the role of CD226 in the function of tumor-infiltrating lymphocytes (TILs) and resistance to immunotherapy. In murine tumors, a large proportion of CD8+ TILs had decreased surface expression of CD226 and exhibited features of dysfunction, whereas CD226hi TILs were highly functional. This correlation was seen also in TILs isolated from HNSCC patients. Mutation of CD226 at tyrosine 319 (Y319) led to increased CD226 surface expression, enhanced anti-tumor immunity and improved efficacy of immune checkpoint blockade (ICB). Mechanistically, tumor-derived CD155, the ligand for CD226, initiated phosphorylation of Y319 by Src kinases, thereby enabling ubiquitination of CD226 by CBL-B, internalization, and proteasomal degradation. In pre-treatment samples from melanoma patients, CD226+CD8+ T cells correlated with improved progression-free survival following ICB. Our findings argue for the development of therapies aimed at maintaining the expression of CD226.


Assuntos
Antígenos de Diferenciação de Linfócitos T/imunologia , Linfócitos T CD8-Positivos/imunologia , Receptores Virais/imunologia , Animais , Linhagem Celular , Linhagem Celular Tumoral , Células HEK293 , Humanos , Inibidores de Checkpoint Imunológico/imunologia , Imunoterapia/métodos , Células Jurkat , Linfócitos do Interstício Tumoral/imunologia , Masculino , Melanoma/imunologia , Camundongos , Camundongos Endogâmicos C57BL
6.
Bioorg Med Chem Lett ; 30(18): 127394, 2020 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-32717611

RESUMO

Hypochlorous acid (HOCl) is an important signaling molecule for cell survival. However, it has been reported that excessive HOCl contributes to a variety of diseases such as cancers. And in cancer cells, the level of HOCl is much higher than that in normal cells. Here a coumarin-based fluorescent probe 7-Diethylamino-3-(2,3-dihydro-1H-perimidin-2-yl)-chromen-2-one (CAN) was successfully developed for HOCl detection. The probe could be oxidized by HOCl to induce significant change in its fluorescence profile, which made it feasible for ratiometric detecting HOCl. CAN (below 1 µM) did not affect cell viability and had good capacity in ratiometric detection of HOCl in RAW 264.7 cells. CAN induced A549 apoptosis and inhibited tumor growth in vitro and in vivo. And CAN could decrease the chlorination activity of myeloperoxidase (MPO) in A549. These findings suggested that CAN (below 1 µM) would develop into a HOCl probe. High activity of MPO and level of HOCl might be helpful for A549 survival. A549 could be induced apoptosis by reducing the HOCl level by CAN. It implies a new anticancer strategy by targeting HOCl.


Assuntos
Cumarínicos/síntese química , Corantes Fluorescentes/síntese química , Halogenação/efeitos dos fármacos , Ácido Hipocloroso/análise , Peroxidase/metabolismo , Células A549 , Animais , Apoptose , Cátions/química , Cumarínicos/metabolismo , Corantes Fluorescentes/metabolismo , Humanos , Ácido Hipocloroso/metabolismo , Metais/química , Camundongos , Imagem Óptica , Oxirredução , Células RAW 264.7 , Transdução de Sinais
7.
Nat Plants ; 6(6): 718, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32427960

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

8.
Trends Plant Sci ; 25(6): 604-617, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32407699

RESUMO

Calcium (Ca2+) serves as an essential nutrient as well as a signaling agent in all eukaryotes. In plants, calcineurin B-like proteins (CBLs) are a unique group of Ca2+ sensors that decode Ca2+ signals by activating a family of plant-specific protein kinases known as CBL-interacting protein kinases (CIPKs). Interactions between CBLs and CIPKs constitute a signaling network that enables information integration and physiological coordination in response to a variety of extracellular cues such as nutrient deprivation and abiotic stresses. Studies in the past two decades have established a unified paradigm that illustrates the functions of CBL-CIPK complexes in controlling membrane transport through targeting transporters and channels in the plasma membrane and tonoplast.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Cálcio/metabolismo , Sinalização do Cálcio , Proteínas de Ligação ao Cálcio/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo
9.
Nat Plants ; 6(4): 384-393, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32231253

RESUMO

Potassium (K) is an essential nutrient, but levels of the free K ions (K+) in soil are often limiting, imposing a constant stress on plants. We have discovered a calcium (Ca2+)-dependent signalling network, consisting of two calcineurin B-like (CBL) Ca2+ sensors and a quartet of CBL-interacting protein kinases (CIPKs), which plays a key role in plant response to K+ starvation. The mutant plants lacking two CBLs (CBL2 and CBL3) were severely stunted under low-K conditions. Interestingly, the cbl2 cbl3 mutant was normal in K+ uptake but impaired in K+ remobilization from vacuoles. Four CIPKs-CIPK3, 9, 23 and 26-were identified as partners of CBL2 and CBL3 that together regulate K+ homeostasis through activating vacuolar K+ efflux to the cytoplasm. The vacuolar two-pore K+ (TPK) channels were directly activated by the vacuolar CBL-CIPK modules in a Ca2+-dependent manner, presenting a mechanism for the activation of vacuolar K+ remobilization that plays an important role in plant adaptation to K+ deficiency.


Assuntos
Arabidopsis/metabolismo , Sinalização do Cálcio , Potássio/metabolismo , Adaptação Fisiológica , Arabidopsis/genética , Arabidopsis/fisiologia , Proteínas de Arabidopsis/genética , Proteínas de Ligação ao Cálcio/genética , Homeostase , Mutação , Fenótipo , Plantas Geneticamente Modificadas , Tabaco/genética , Vacúolos/metabolismo
10.
Chempluschem ; 85(4): 742-750, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32329586

RESUMO

A series of indole-based helicenes, namely, 15-hexyl-15H-tetraphenyl[1,2-e]indole (HTPI), 14-hexyl-14H-benzo[4',5']thieno [2',3':7,8]naphtha[1,2-e]indole (HBTNI), 7-hexyl-7H-indolo[5,4-k] phenanthridine (HIPD) and 3-hexyl-3H-phenanthro[4,3-e]indole (HPI) were successfully synthesized by three- or four-step reactions. They exhibited good solubility and high thermal stability of Td =247, 388, 294 and 251 °C, respectively. These compounds emit violet-blue light with maximum emission peaks at 415, 397, 397 and 391 nm in hexane. Among them, HBTNI had excellent thermal stability, narrow and sharp emission peaks, and highest photoluminescence quantum yield (0.32). Thus, HBTNI was an ideal candidate for the violet-blue emitters of OLEDs. Furthermore, the four compounds had two-photon absorption and two-photon excited fluorescence. HTPI showed the maximum TPA with the TPA cross-section (δ) of 171.5 GM at 770 nm. They were rare examples of helicenes with both violet-blue emission and TPA.

11.
Materials (Basel) ; 13(2)2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31936537

RESUMO

In order to investigate the bond behavior of preplaced aggregate concrete-filled steel tube (CFT-PAC) columns and the difference of bond behavior between CFT-PAC columns and normal concrete-filled steel tube (CFT-NC) columns, a total of 11 columns were prepared and the push-out tests were conducted. The experimental parameters included the type of concrete (preplaced aggregate concrete and normal concrete), concrete strength (C40, C50 and C60), cross-section dimension (D = 219 mm, 299 mm and 351 mm) and the thickness of steel tube (t = 6 mm and 8 mm). The results indicated that the CTF-PAC columns had a similar load-slip curves with CFT-NC columns. The bond stresses of the CFT-PAC columns were higher than that of the PAC-NC columns at the same concrete strength. Increasing compressive strength of PAC increased the critical bond strength and bond strength of CFT-PAC columns. With an increase of the L/D ratio, both of the slip corresponding to peak load and bond strength of CFT-PAC columns exhibited an increasing trend. A rise in the D/t ratio led to a decrease in the bond stress of CFT-PAC columns and an increase in slip corresponding to the peak load of CFT-PAC columns. The proposed bond stress-slip relationship model considerably matched the bond stress-slip relationship of CFT-PAC columns.

12.
Chem Biol Drug Des ; 95(3): 380-387, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31442363

RESUMO

Phosphatidylcholine-specific phospholipase C (PC-PLC) is one of the important members of phospholipase family which is capable of specifically hydrolyzing the third phosphate linker of glycerophospholipid molecules, releasing phosphocholine and diacylglycerols (DAG). It is a crucial virulence factor of bacteria contributed to cell-to-cell spread and leading multiple diseases in mammals. Moreover, PC-PLC has a wide range of biological functions and involves in various cell signaling pathway, including apoptosis, proliferation, differentiation, and metastasis. In this study, we have synthesized 2 chiral compounds ((R)-7-amino-2,3,4,5-tetrahydrobenzo[b][1,4]oxazepin-3-ol, called R-7ABO, and (S)-7-amino-2,3,4,5-tetrahydrobenzo[b][1,4]oxazepin-3-ol, called S-7ABO) and discovered their inhibitory effect on PC-PLC activity which derived from Bacillus cereus (B. cereus) and human umbilical vein endothelial cells (HUVEC). Therefore, as two novel efficient PC-PLC inhibitors, R-7ABO and S-7ABO might become favorable tools of antibacterial therapy in B. cereus infection diseases and researching the function of PC-PLC in HUVECs.


Assuntos
Inibidores Enzimáticos/química , Inibidores Enzimáticos/metabolismo , Fosfolipases Tipo C/antagonistas & inibidores , Apoptose , Bacillus cereus , Diferenciação Celular , Proliferação de Células , Diglicerídeos/química , Diglicerídeos/metabolismo , Glicerofosfolipídeos/química , Glicerofosfolipídeos/metabolismo , Células Endoteliais da Veia Umbilical Humana , Humanos , Hidrólise , Fosforilcolina/química , Fosforilcolina/metabolismo , Transdução de Sinais , Estereoisomerismo , Relação Estrutura-Atividade
13.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 45(12): 1389-1397, 2020 Dec 28.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-33472993

RESUMO

OBJECTIVES: To investigate the effect of adriamycin (ADM), idelalisib or ADM and their combination on cell proliferation and intracellular concentration of ADM, and to explore the reversal effect of idelalisib on drug resistance to ADM. METHODS: The K562 and K562/ADM cells were respectively treated with ADM and idelalisib at different concentrations. The 50% inhibitory concentration (IC50) and drug resistance index (RI) of ADM to the 2 kinds of cells were measured by methyl thiazolyl tetrazolium (MTT) assay. Non-cytotoxic dose (cell inhibition rate <5%) of idelalisib in the 2 kinds of cells was determined. Then the K562 and k562/ADM cells were divided into the following groups: a K562 cells + ADM group, a K562 cells + ADM + idelalisib group, a K562/ADM cells + ADM group, and a K562/ADM cells + ADM + idelalisib group. The survival rates, the intracellular ATP levels, and the relative concentration of intracellular ADM were detected by MTT method, ATP bioluminescence assay (ATP-BLA) and flow cytometry (FCM), respectively. RESULTS: The cell survival rates were significantly decreased in a dose-dependent manner when the cells were treated with different doses of ADM (0.001-10.000 mg/L ). The IC50 value of ADM in the K562 and K562/ADM cells were 0.2 mg/L and 1.0 mg/L, respectively. The RI value was 5. The cell survival rates were also significantly decreased in a dose-dependent manner when the cells were treated with different doses of idelalisib (1-50 µmol/L). The non-cytotoxic dose of idelalisib in the K562 and K562/ADM cells were 25 µmol/L and 15 µmol/L, respectively. The cell survival rates in the ADM+ idelalisib group was less than that in the ADM group (P<0.05);while there was no statistical difference between the ADM+ idelalisib group and the ADM group in the K562 cells (P>0.05). The intracellular ATP level in the K562 cells was about (91.502±0.479) mmol/L, and that in the K562/ADM cells was about (24.311±0.349) mmol/L. The intracellular ATP level in the ADM+ idelalisib group in the K562/ADM cells was less than that in the ADM group (P<0.05); but there was no statistical difference between the ADM + idelalisib group and the ADM group in the K562 cells (P>0.05). The absorption of intracellular ADM in the ADM + idelalisib group in the K562/ADM cells was more than that in the ADM group (P<0.05); but there was no statistical difference in the K562 cells between the 2 groups (P>0.05). The exclusion of intracellular ADM in the ADM + idelalisib group in the K562/ADM cells was less than that in the ADM group (P<0.05 or P<0.01);but there was no statistical difference in the K562 cells between the 2 groups (P>0.05). CONCLUSIONS: Idelalisib exerts effect on inhibition of the proliferation in myeloid leukemia K562 and K562/ADM cells, which may partially reverse the drug resistance of K562/ADM cells to ADM. The mechanisms for the effect of idelalisib may be related to increasing the accumulation of ADM and inducing the cell apoptosis in the K562 and K562/ADM cells.


Assuntos
Doxorrubicina , Leucemia Mieloide , Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Proliferação de Células , Doxorrubicina/farmacologia , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Humanos , Células K562 , Purinas , Quinazolinonas
14.
Materials (Basel) ; 12(24)2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31817442

RESUMO

The shrinkage properties of self-compacting rubber lightweight aggregate concrete (SCRLC) were investigated by experimental and analytical studies in this paper. Rubber particles were used to substitute the sand in SCRLC by volume and rubber particles substitution percentages were 10%, 20%, 30%, 40% and 50%. The experimental results showed that the shrinkage strains of SCRLC increased with an increase of rubber particles substitution percentage. On the basis of China Academy of Building Research model and experimental data, a predictive model for describing the shrinkage strains of SCRLC was established. After analytical study, it was revealed that rubber particles substitution percentage of 80% in SCRLC might be the upper limit of substitution percentage; if the rubber particles substitution percentage was larger than 80%, the shrinkage strains of SCRLC would exceed the limiting value stipulated in JGJ51-2002 and the durability of SCRLC would drop to a worse status.

15.
Materials (Basel) ; 12(24)2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31817506

RESUMO

The recycling of waste tires in lightweight aggregate concrete (LC) would achieve huge environmental and societal benefits, but the effects of rubber particles on the partial properties of LC are not clear (e.g., the stress-strain relationship). In this paper, uniaxial compressive experiments were conducted to evaluate the stress-strain relationship of self-compacting rubber lightweight aggregate concrete (SCRLC). Rubber particles were used to replace sand by volume, and substitution percentages of 0%, 10%, 20%, 30%, 40%, and 50% were set as influence factors. Experimental results indicate that with increased rubber particles substitution percentage, the cubic compressive strength and axial compressive strength of SCRLC decreased, while the failure modes of SCRLC prism specimens gradually changed from brittle to ductile failure. As the rubber particles substitution percentage increased from 0% to 50%, the peak strain of SCRLC increased whereas peak stress, elastic modulus, and peak secant modulus of SCRLC deceased, the descending stage of stress-strain curves became softer. The rubber particles substitution percentage of 30% was the critical point at which an obvious change in the properties of SCRLC occurred. Based on the data collected from experimental studies, a predictive model for SCRLC was established and a further prediction of the SCRLC stress-strain relationship was given.

16.
Med Sci Monit ; 25: 8671-8682, 2019 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-31734687

RESUMO

BACKGROUND Primary lymphoma of the breast is rare, and primary diffuse large B cell lymphoma (DLBCL) of the breast is very rare. This study aimed to identify the clinicopathological characteristics and treatment associated with prognosis in patients with primary DLBCL of the breast. MATERIAL AND METHODS A retrospective study included the clinical and treatment data from 46 women with a histological diagnosis of primary DLBCL. Patients were staged using Ann Arbor staging criteria, overall survival (OS), progression-free survival (PFS), and the international prognostic index (IPI) scores were obtained. Laboratory finding included serum lactate dehydrogenase (LDH), and the immunohistochemistry findings were recorded. RESULTS Patients (n=46), included stage I (n=18), stage II (n=18), stage III (n=3), and stage IV DLBCL (n=9). Treatment included chemotherapy with rituximab (n=16), and radiotherapy (n=12). The median follow-up time was 40.5 months, the 5-year OS rate was 36.2%, and the 5-year PFS rate was 29.1%. Univariate analysis showed that clinical stage, serum LDH, the IPI score, chemotherapy cycles >3, and Bcl-2 and Bcl-6 expression were correlated with the 5-year OS and PFS. Multivariate risk regression analysis showed that the number of chemotherapy cycles (>3) and Bcl-6 expression were independent prognostic factors in primary DLBCL of the breast (P<0.05). CONCLUSIONS A retrospective study of 46 patients with primary DLBCL of the breast showed that >3 cycles of chemotherapy and expression of Bcl-6 resulted in improved OS and PFS. Radiotherapy controlled local tumor recurrence but did not improve the OS and PFS. Rituximab did not improve patient survival.


Assuntos
Linfoma Difuso de Grandes Células B/mortalidade , Linfoma Difuso de Grandes Células B/patologia , Linfoma Difuso de Grandes Células B/terapia , Adulto , Idoso , Mama/patologia , China , Intervalo Livre de Doença , Feminino , Humanos , Imuno-Histoquímica/métodos , Estimativa de Kaplan-Meier , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Intervalo Livre de Progressão , Estudos Retrospectivos , Rituximab/farmacologia , Taxa de Sobrevida
17.
Am J Transl Res ; 11(8): 4935-4945, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31497210

RESUMO

Circular RNAs (circRNAs) belong to non-coding RNAs and are known as key regulators in gene regulation. CircRNAs involve in the various biological processes of cancer. However, the functions of circRNAs in renal cell carcinoma (RCC) are still not clear. In this study, the circRNA expression profile was performed in the RCC tissues by microarray. There were 35 significantly expressed circRNAs with more than 5 folds from microarray analysis. Hsa_circ_0039569 (circ_0039569) was verified to be up-regulated in RCC and cells compared with the controls by real time RT-PCR. The assays of cellular functions showed that circ_0039569 down-regulation suppressed the proliferation and metastasis of RCC cells. The molecular mechanism of circ_0039569 in RCC cells showed that circ_0039569 promoted RCC progression by up-regulating CCL22 expression via down-regulating miR-34a-5p. Taken together, the study indicated that circ_0039569 played an important role in RCC cell survival and metastasis, which suggested that an oncogenic role of circ_0039569 in RCC progression.

18.
In Vitro Cell Dev Biol Anim ; 55(5): 349-354, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30989449

RESUMO

In our previous study, we proved that a novel Heat shock protein 90 (HSP90) inhibitor 4-(3-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)-5-phenyl-4,5-dihydro-1H-pyrazol-1-yl) benzoic acid (DPB) could inhibit A549 lung cancer cell growth via inducing apoptosis. However, whether DPB affects autophagy is still unknown. Here, we investigated the effects of DPB on autophagy and the improved anti-cancer activity in A549 lung cancer cells. Aggregation of LC3-II was observed using laser scanning confocal microscopy in GFP-LC3 stably transfected U87 cells. Autophagy and apoptosis-related protein levels were examined by Western blot analysis. It is suggested that treatment with DPB (5-20 µmol/L) induced mTOR-independent autophagy in dose- and time-dependent manners. Pre-treatment A549 cells with autophagy inhibitor 3-methyladenine (3-MA, 5 mmol/L) enhanced DPB-induced apoptosis. And, DPB inhibited A549 cell growth more effectively in combination with autophagy inhibitors 3-MA (5 mmol/L) or 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO, 30 µmol/L). These results illustrated that as a potential and promising HSP90 inhibitor, DPB could be utilized in the treatment of cancer combined with the autophagy inhibitor.


Assuntos
Adenocarcinoma de Pulmão/tratamento farmacológico , Autofagia/efeitos dos fármacos , Proteínas de Choque Térmico HSP90/genética , Tioureia/análogos & derivados , Células A549 , Adenocarcinoma de Pulmão/genética , Adenocarcinoma de Pulmão/patologia , Apoptose/efeitos dos fármacos , Autofagia/genética , Proliferação de Células/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Proteínas de Choque Térmico HSP90/antagonistas & inibidores , Humanos , Transdução de Sinais , Tioureia/farmacologia
19.
Zhongguo Zhong Yao Za Zhi ; 44(23): 5088-5093, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-32237342

RESUMO

In order to study the pesticide residues of the medicinal Crataegi Fructus,this study aims to establish an analysis method for pesticide residues( mainly containing insecticides and fungicides) suitable for the actual situation of medicinal Crataegi Fructus based on the survey of the pesticides of the Crataegi Fructus base,combined with the blind screening results of the LC-ESI-MS/MS pesticide screening platform established by the research team in the early stage. Then,the pesticide residues in medicinal Crataegi Fructus from Shandong,Hebei,Henan,Shanxi,and Liaoning( main cultivation areas) were analyzed. The samples were pretreated by the modified Qu ECh ERS method,i.e.,extracted with acetonitrile-water( 9 ∶1),purified by PSA,C_(18),GCB,silica gel. The detection of pesticides was performed by LC-MS/MS. The ion source was ESI with positive scanning mode,and the linearity of 11 kinds of pesticides in the range of 5-300 µg·kg~(-1) was acceptable( R~2>0. 996 9). All the recoveries of pesticides were within 70. 02%~(-1)12. 0% in the low,medium and high levels,with RSD≤17%. The results showed that the detection rate of carbendazim,chlorpyrifos and difenoconazole is 79%,82%,56%,respectively. Besides,the prohibition pesticide carbofuran were detected in some of the batches,indicating the security risk. This study provides methodological references and basic data for risk assessment of Crataegi Fructus and government regulation.


Assuntos
Crataegus/química , Contaminação de Medicamentos , Medicamentos de Ervas Chinesas/análise , Resíduos de Praguicidas/análise , Cromatografia Líquida , Inquéritos e Questionários , Espectrometria de Massas em Tandem
20.
Acta Crystallogr E Crystallogr Commun ; 74(Pt 12): 1878-1880, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30574392

RESUMO

The title compound, tris-(1,1'-dibutyl-4,4'-bi-pyridine-1,1'-diium) bis-(dimethyl sulfoxide)di-µ3-iodido-tetra-µ2-iodido-octa-iodido-tetra-lead(II) dimethyl sulfoxide di-solvate, (C18H26N2)3[Pb4I14(C2H6OS)2]·2C2H6OS, belongs to a class of organic-inorganic hybrid materials with novel functionalities. In this compound, C-H⋯O and C-H⋯I hydrogen-bonding inter-actions, π-π inter-actions, other short contacts and Pb octa-hedral chains are present, extending the crystal structure into a three-dimensional supra-molecular network.

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