Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 5.833
Filtrar
1.
Clin Exp Dermatol ; 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34260092

RESUMO

BACKGROUND: Educational videos improve patient knowledge of wound care and skin cancer. However, the effect of viewing an educational video at home prior to Mohs surgery has not been demonstrated. OBJECTIVE: To evaluate the use of an educational video to improve patient understanding of MMS MATERIALS AND METHODS: Patients scheduled to undergo MMS were randomized to receive standard patient education, or standard patient education with an additional video developed by the authors. The educational material was mailed to patients along with the details of their MMS appointment. Both groups answered questionnaires to assess their knowledge of MMS, as well as their anxiety and satisfaction. RESULTS: Patients that watched the educational video scored higher on the knowledge questionnaire than patients in the control group (0.8, 95% CI 0.3 to 1.4, p = 0.003), but were not statistically less anxious (-0.7, 95% CI -2.6 to 1.3, p = 0.50). Overall, patients undergoing MMS were satisfied. CONCLUSION: Home viewing of an educational video prior to MMS can improve patient understanding.

2.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 33(3): 267-273, 2021 Jun 30.
Artigo em Chinês | MEDLINE | ID: mdl-34286528

RESUMO

OBJECTIVE: To identify the environmental factors affecting the geographical distribution of Rhipicephalus microplus in China, and to examine the impact of climate changes on the distribution of R. microplus in China. METHODS: The national and international publications pertaining to the geographical distribution of R. microplus in China were retrieved, and the geographical location was extracted. The suitable habitats of R. microplus and the dominant environmental factors affecting the distribution of suitable habitats of R. microplus were predicted in China based on the geographical data and environmental variables using the ArcGIS 10.7 software and the maximum entropy model. RESULTS: Among the main climatic factors affecting the geographical distribution of R. microplus in China, the factors contributing more than 10% to the suitable habitats of R. microplus mainly include the annual mean precipitation (38.2%), the average temperature of the coldest quarter (28.4%) and the precipitation of the driest month (14.2%). The current suitable habitats of R. microplus were mainly found in southern China, and the high-, medium- and low-suitable areas accounted for 8.6%, 13.1% and 10.5% of the total land area of China, respectively. The suitable habitats of R. microplus were predicted to increase by 399 800 km2 in China using the maximum entropy model under the RCP 4.5 emissions scenario in 2070, and the emerging suitable habitats were mainly distributed in Gansu Province, Ningxia Hui Autonomous Region, Qinghai Province, Hebei Province, Shaanxi Province, Liaoning Province, Inner Mongolia Autonomous Region, Shandong Province, Sichuan Province and Tibeten Autonomous Region. In addition, the suitable habitats of R. microplus were predicted to show an overall expansion towards northward from present to 2070. CONCLUSIONS: Climate changes affect the distribution of suitable habitats of R. microplus in China, and annual mean precipitation may be a key factor affecting the distribution of suitable habitats of R. microplus.


Assuntos
Mudança Climática , Rhipicephalus , Animais , China , Ecossistema , Temperatura
3.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 33(3): 281-286, 2021 Jun 21.
Artigo em Chinês | MEDLINE | ID: mdl-34286530

RESUMO

OBJECTIVE: To identify the environmental factors affecting the distribution of suitable habitats of Ixodes ovatus, and to examine the effects of environmental changes on the distribution of suitable habitats of I. ovatus. METHODS: Data pertaining to the distribution of I. ovatus in China were captured by literature review. The distribution of suitable habitats of I. ovatus in China was simulated using the maximum entropy model with the environmental variable data and the I. ovatus distribution data. In addition, the potential distribution of suitable habitats of I. ovatus was predicted based on the 2050 and 2070 environmental data. RESULTS: The current suitable habitats of I. ovatus cover 3.11 million km2, accounting for 32.28% of the total land area of China, and they are mainly concentrated in southwestern and northwestern China. Among the screened 16 environmental factors, the standard deviation of seasonal variation of temperature, average annual precipitation and altitude were predominant environmental factors affecting the distribution of suitable habitats of I. ovatus, and these three variables contributed 76.5% to the distribution of suitable habitats of I. ovatus in China. The suitable habitats of I. ovatus were predicted to showing a tendency towards expansion to northwestern and northeastern China in 2050 and 2070, and the proportion of highly suitable habitats of I. ovatus was predicted to increase. CONCLUSIONS: Moderate climate, adequate precipitation and high altitude are favorable for the survival of I. ovatus. Future climate changes may cause expansion of suitable habitats of I. ovatus in China.


Assuntos
Ixodes , Altitude , Animais , China , Mudança Climática , Ecossistema
4.
Zhonghua Wei Chang Wai Ke Za Zhi ; 24(7): 560-566, 2021 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-34289537

RESUMO

The mesentery is a continuous unity and the operation of digestive carcinoma is the process of mesenteric resection. This paper attempts to simplify the formation process of all kinds of fusion fascia in the process of digestive tract embryogenesis, and to illuminate the continuity of fusion fascia with a holistic concept. This is helpful for beginners to reversely dissect the fusion fascia and maintain the correct surgical plane during operation, and to achieve the purpose of complete mesenteric resection.


Assuntos
Neoplasias do Colo , Neoplasias Gastrointestinais , Laparoscopia , Mesocolo , Neoplasias do Colo/cirurgia , Neoplasias Gastrointestinais/cirurgia , Humanos , Mesentério/cirurgia
5.
Rev Sci Instrum ; 92(5): 053702, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243274

RESUMO

This paper discusses a laser monitor with two channels for acquiring images synchronized in time and its use for visualizing the surface of metal nanopowders during combustion. Each channel is an independent optical system based on a copper bromide vapor brightness amplifier and a high-speed camera that allows visualizing a specified area of the sample. Thus, the laser monitor allows real-time imaging of the same sample area with different spatial resolutions and simultaneously two areas of the sample. In comparison with the "master oscillator-power amplifier" system and a bistatic laser monitor in the proposed system, the radiation pulses of the brightness amplifiers are synchronized in such a way that the inversion in the active media is formed at different time intervals. Such a system makes it possible to register processes in one area of the sample in the mode of double frames with a time shift of 35 ns. As an additional option in the proposed system, it is possible to implement a laser illumination mode in which an additional camera is used with a narrow-band filter installed on it for the emission wavelength of brightness amplifiers, and the same radiation from the brightness amplifier is used as an illuminating light source. The proposed technique was used to visualize the combustion of aluminum nanopowders without additives in real time in the area of laser initiation with different spatial resolutions and nanoAl + microAl + microFe powder mixtures in the laser ignition area and the adjacent area of the sample simultaneously as the combustion wave propagates.

6.
Artigo em Chinês | MEDLINE | ID: mdl-34256483

RESUMO

Objective: To explore the otological and hearing characteristics in children with Turner syndrome (TS), to determine risk factors of hearing loss, and to discuss algorithms for future surveillance. Methods: The clinical data of otolaryngology in children with TS from January 2018 to April 2020 were reviewed retrospectively. A total of 46 female children with TS, aged from 5 to 18 years were enrolled. Karyotypes included 17 cases (37.0%) of monosomy, 16 cases (34.7%) of mosaicism, and 13 cases (28.3%) of abnormal X chromosome structures. The otoscopic characteristics, audiological performance, and otologic diagnoses were evaluated. Multivariate logistic regression and Mann-Whitney U test were used for statistical analysis. Results: The most common otologic comorbidity was otitis media with effusion (OME), including 20 cases (43.5%) with 33 ears (35.9%). 14 cases (30.4%) were diagnosed with hearing loss. The regression analysis revealed that the age (OR=1.345, 95%CI: 1.072-1.760) and comorbidity of OME (OR=9.460, 95%CI: 2.065-60.350) were risk factors associated with hearing loss. In TS with OME, when compared with the mean air conduction threshold, the hearing loss of the group with pars flaccida retractions was significantly higher ((24.3±13.8) dB HL vs. (14.4±4.2) dB HL, U=59.500, P=0.008) than that of the group with none retractions. Conclusion: OME and hearing loss are common in children with TS. As age increases or suffers from OME, the risk of hearing loss increases.


Assuntos
Otite Média com Derrame , Síndrome de Turner , Criança , Feminino , Audição , Testes Auditivos , Humanos , Estudos Retrospectivos , Síndrome de Turner/complicações , Síndrome de Turner/epidemiologia
7.
Zhonghua Shao Shang Za Zhi ; 37(7): 640-646, 2021 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-34304404

RESUMO

Objective: To investigate the expression and phosphorylation level change of adenosine monophosphate activated protein kinase (AMPK) in skeletal muscle of severely scald rats and its roles in skeletal muscle atrophy in severely scalded rats. Methods: The experimental research method was applied. Totally 100 6-week-old male Wistar rats were divided into sham injury group and scald group according to the random number table, with 50 rats in each group. After weighing the body weight, rats in scald group were inflicted with full-thickness scald of 30% total body surface area on the back, and rats in sham injury group were simulated with scald. At 6 h and on 1, 3, 5, and 7 d post injury, 10 rats in each group were taken to measure their body weights and weights of extensor digitorum longus and soleus muscle. At 6 h and on 1, 3, 5, and 7 d post injury, the tibialis anterior muscles were collected, the mRNA expressions of muscle atrophy F-box protein (MAFbx) and muscle-specific RING finger protein 1 (MuRF1) were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction; the content of adenosine monophosphate (AMP), adenosine diphosphate, and adenosine triphosphate (ATP) were detected by high performance liquid chromatography, and AMP/ATP ratio and energy charge were calculated; the protein expressions of AMPK-α and phosphorylated AMPK-α (p-AMPK-α) were detected by Western blotting, and the p-AMPK-α/AMPK-α ratio was calculated, with sample number of 4 in each time point of each group. Data were statistically analyzed with analysis of variance for factorial design and least significant difference test. Results: The body weights of rats in 2 groups before injury and at each time point post injury were close (P>0.05). At 6 h post injury, the weight of extensor digitorum longus of rats in scald group was (0.107±0.007) g, which was significantly heavier than (0.086±0.0607) g of sham injury group (P<0.01). On 3 d post injury, the weight of extensor digitorum longus of rats in scald group was (0.083±0.016) g, which was significantly lighter than (0.102±0.005) g of sham injury group (P<0.01). The weight of soleus of rats in 2 groups were close at each time point post injury (P>0.05). Compared with those of sham injury group, the mRNA expression of MAFbx in tibialis anterior muscle of rats in scald group was significantly up-regulated at 6 h post injury (P<0.01), and the mRNA expressions of MuRF1 in tibial anterior muscle of rats in scald group were significantly up-regulated at 6 h and on 1 d post injury (P<0.01). At 6 h and on 7 d post injury, compared with those of false injury group, the AMP/ATP ratios of the tibial anterior muscle of rats in scald group were significantly increased (P<0.05 or P<0.01), and energy charges of the tibial anterior muscle of rats in scald group were significantly decreased (P<0.01). At each time point post injury, the protein expressions of AMPK-α of the tibial anterior muscle of rats in 2 groups were close (P>0.05). The p-AMPK-α/AMPK-α ratios of the tibial anterior muscle of rats in scald group at 6 h and on 7 d post injury were significantly higher than those in sham injury group (P<0.05 or P<0.01). Conclusions: The decrease in energy charge and increase in AMP/ATP ratio of skeletal muscle of rats after severe scald activate AMPK. The activation of AMPK in the early stage of injury is consistent with the up-regulation of MAFbx and MuRF1 expressions and down-regulation of skeletal muscle weight. The above-mentioned changes may be one of the molecular mechanisms of skeletal muscle atrophy in rats with severe scald.


Assuntos
Queimaduras , Proteínas Quinases , Monofosfato de Adenosina , Animais , Masculino , Músculo Esquelético , Atrofia Muscular , Ratos , Ratos Sprague-Dawley , Ratos Wistar
8.
Zhonghua Fu Chan Ke Za Zhi ; 56(7): 458-466, 2021 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-34304437

RESUMO

Objective: To evaluate the value of whole exome sequencing (WES) in prenatal clinical application. Methods: A total of 1 152 cases of congenital abnormal [including structural malformation, nuchal translucency (NT) thickening and intrauterine growth restriction] with traditional prenatal diagnosis [including G-band karyotype analysis and chromosome microarray analysis (CMA)] negative were analyzed. The congenital abnormal fetuses were divided into retrospective group and prospective group according to the time of WES detection, that is whether the pregnancy termination or not. According to the specific location of fetal malformation and their family history, the cohort was divided into subgroups. The clinical prognosis of all fetuses were followed up, and the effect of WES test results on pregnancy decision-making and clinical intervention were analyzed. According to the follow-up results, the data of fetuses with new phenotypes in the third trimester or after birth were re-analyzed. Results: Among 1 152 families who received WES, 5 families were excluded because of nonbiological parents. Among the remaining 1 147 families, 152 fetuses obtained positive diagnosis (13.3%,152/1 147), including 74 fetuses in the retrospective group (16.1%,74/460) and 78 fetuses in the prospective group (11.4%,78/687). In fetuses with negative CMA and G-band karyotype analysis results but new phenotypes in the third trimester or after birth, the positive rate by WES data re-analysis was 4.9% (8/163). A total of 34 (21.3%, 34/160) fetuses were directly affected by the corresponding positive molecular diagnosis. Among 68 cases of live births with diagnostic variation grade 4, 29 cases (42.7%, 29/68) received appropriate medical intervention through rapid review of WES results. Conclusions: WES could increase the detection rate of abnormal fetuses with negative G-banding karyotype analysis and CMA by 13.3%. Prenatal WES could guide pregnancy decision-making and early clinical intervention. It might be an effective strategy to pay attention to the special follow-up of the third trimester and postnatal fetus and to re-analyze the WES data.


Assuntos
Anormalidades Congênitas , Diagnóstico Pré-Natal , Anormalidades Congênitas/diagnóstico , Anormalidades Congênitas/genética , Feminino , Feto/diagnóstico por imagem , Humanos , Medição da Translucência Nucal , Gravidez , Estudos Prospectivos , Estudos Retrospectivos , Ultrassonografia Pré-Natal , Sequenciamento Completo do Exoma
9.
Zhonghua Fu Chan Ke Za Zhi ; 56(7): 489-497, 2021 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-34304441

RESUMO

Objective: To investigate the correlations of laminin subunit gamma 3 (LAMC3) expression with prognosis of ovarian cancer (OC). Methods: LAMC3 protein expression was measured using immunohistochemical streptavidin-peroxidase-biotin connection method (IHC). Gene expression and related clinical data in the cancer genome atlas (TCGA) cohort and clinical proteomic tumor analysis consortium (CPTAC) were applied to analyse the correlation between gene and protein expressions and clinical outcomes. Correlations between LAMC3 and clinicopathological factors were evaluated using the Pearson χ2 test (2-sided). The probability of survival and significance was calculated using the Kaplan-Meier plot. The functional clustering of biological pathways enriched from co-expressed genes of LAMC3 was used to explore the possible mechanisms that LAMC3 might contribute to poor prognosis. Results: Based on the IHC results of 216 OC tissues or ovaries (including 208 tumors and 8 normal tissues) and 51 OC tissues (including 24 chemotherapy-resistant and 27 sensitive tissues), and the protein expression data from CPTAC (including 100 primary tumors and 25 normal tissues), the results showed that the protein expression of LAMC3 was significantly decreased in OC tissues compared with normal, decreased in advanced-stage tissues compared with early-stage tissues, and decreased in drug-resistant tissues compared with sensitive tissues (all P<0.05). Furthermore, low expression of LAMC3 protein was significantly associated with poor disease-free survival (DFS) and overall survival (OS) in 51 OC tissues (P<0.01), consistent with the results that the low levels of LAMC3 mRNA predicted short DFS and OS in 489 OC tissues of the TCGA cohort (P<0.05). The results suggested that low expression of LAMC3 might be the adverse factors for OC development, such as drug resistance and advanced tumors, and might be a risk indicator for prognosis. Moreover, functional clustering of biological pathways enriched from the co-expressed genes of LAMC3 in TCGA ovarian cohort indicated that LAMC3 potentially involved in regulation of OC via oncogene-pathways such as Ras associated protein 1 (Rap1), mitogen-activated protein kinase (MAPK), Ras and cell adhesion-related pathways such as extra cellular matrix (ECM)-receptor interaction and focal adhesion. It indicated that LAMC3 might contribute to short survival and tumor progression by regulation of the above pathways. Conclusion: Low expression of LAMC3 is related to poor prognosis and malignant progression in OC, and thus it is expected to be a new prognostic marker and therapeutic target for clinical treatment.


Assuntos
Neoplasias Ovarianas , Proteômica , Biomarcadores Tumorais/genética , Carcinoma Epitelial do Ovário , Feminino , Humanos , Laminina , Neoplasias Ovarianas/genética , Prognóstico
10.
Nan Fang Yi Ke Da Xue Xue Bao ; 41(7): 1067-1072, 2021 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-34308858

RESUMO

OBJECTIVE: To investigate the mechanism by which galectin-3 (Gal-3) affects the stability of vulnerable coronary atherosclerosis plaques through long non-coding RNA ARSR (lncARSR). METHOD: Male BALB/c mice were randomly divided into normal diet group, high-fat diet group, high-fat diet+lncARSR inhibitor group (n=20). The high-fat diet contained 15% fat and 0.25% cholesterol, and lncARSR inhibitor was injected intravenously at 50 nmol/L every other day. After 12 weeks of high-fat diet feeding and treatment, the mice were euthanized for analyzing coronary atherosclerosis and plaque damage using Sudan IV and oil red O staining. The protein expressions of Gal-3 and ARSR in the coronary artery of the mice were analyzed with Western blotting, and the expressions of PI3K and Akt were detected with immunohistochemistry. The coronary artery tissues were harvested from normal mice for cell culture, and the isolated cells were transfected with a Gal-3 mimic or a Gal-3 inhibitor. At 24 h after the transfection, dual luciferase reporter gene assay was performed to determine the target relationship between Gal-3 and lncARSR; the mRNA expressions of tumor necrosis factor (TNF-α), interleukin-ß (IL-ß) and IL-6 in the transfected cells were detected with RT-qPCR. RESULTS: The positively stained areas by Sudan IV and red oil O and the protein expression of lncARSR were the lowest in normal diet group and the highest in high-fat diet group (P < 0.05). The protein expression of PI3K and Akt and the mRNA expression of TNF-α, IL-ß and IL-6 in high-fat diet group were higher than those in normal diet group. The protein expression of PI3K and Akt and the mRNA expression of TNF-α, IL-ß and IL-6 in high-fat diet+ lncARSR inhibitor group were significantly lower than those in high-fat diet group (P < 0.05). In the cell experiment, the activity of WT-lncARSR was significantly higher in Gal-3 mimic transfection group than in the control group and Gal-3 inhibition group (P=0.026), and was the lower in Gal-3 inhibition group than in the control group (P=0.017). CONCLUSION: Gal-3 and lncARSR are overexpressed in coronary atherosclerosis. Through a mechanism for targeted inhibition of lncARSR, Gal-3 regulates the PI3K/ Akt signaling pathway to suppress inflammation and thus regulate the stability of vulnerable coronary atherosclerosis plaques.


Assuntos
Doença da Artéria Coronariana , Placa Aterosclerótica , RNA Longo não Codificante , Animais , Dieta Hiperlipídica/efeitos adversos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , RNA Longo não Codificante/genética , Transdução de Sinais
11.
Pulm Pharmacol Ther ; : 102061, 2021 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-34314854

RESUMO

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease distinguished by airway remodelling and progressive inflammation. PAI-1 is an important regulator of fibrosis. Recent studies have shown that PAI-1 seems to be involved in COPD progression. Elevated levels of PAI-1 have been found in the lungs of patients with acute inflammation. PAI-1 has been shown to regulate the levels of proinflammatory cytokines in the lungs, such as tumour necrosis factor (TNF)-α and interleukin (IL)-6, indicating that PAI-1 may play a fundamental role during inflammation. In the present study, we investigated the anti-inflammatory role of baicalin, the main active component of Scutellaria baicalensis, against cigarette smoke (extract) (CS/CSE)-induced airway inflammation in vivo and in vitro. For the in vivo study, SD rats were exposed to CS for 1 hour/day, 6 days/week, for 24 weeks and treated with baicalin (40, 80 and 160 mg/kg) or budesonide (0.2 mg/kg). For this study, HBE cells were pretreated with baicalin (10, 20, 40 µM) or dexamethasone (10-7 M) and then exposed to CSE. We found that baicalin treatment could ameliorate CS-induced airway inflammatory infiltration in rats and decrease PAI-1 expression. The ELISA results showed that baicalin significantly inhibited the levels of TNF-α and IL-1ß in CS/CSE-exposed rats and cells. Mechanistic studies showed that baicalin enhanced histone deacetylase 2 (HDAC2) protein expression and inhibited the expression of NF-κB and its downstream target PAI-1, and these effects were reversed by the HDAC2 inhibitor CAY-10683. In conclusion, baicalin ameliorated CS-induced airway inflammation in rats, and these effects were partially attributed to the modulation of HDAC2/NF-κB/PAI-1 signalling.

12.
Eur Rev Med Pharmacol Sci ; 25(12): 4219-4227, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34227089

RESUMO

OBJECTIVE: The objective of this study was to investigate the role of long noncoding RNAs small nucleolar RNA host gene 17 (SNHG17) in hepatocellular carcinoma (HCC). PATIENTS AND METHODS: Here, the expression level of SHNG17 was determined using reverse transcription quantitative PCR in tissue specimens and cell lines. The chi-squared test was used to analyze the associations between SNHG17 expression and clinical pathological factors in HCC patients. Kaplan-Meier and log-rank analyses were used to evaluate the prognosis of HCC patients, and proportional hazards model (Cox) regression was utilized for univariate and multivariate analyses. Knockdown of SNHG17 was achieved by transfection with si-SNHG17 in HepG2 and SNU-182 cells. Cell function was analyzed using CCK-8 assay, colony formation assay, Flow cytometry analysis and transwell assays. RESULTS: Our data showed that SNHG17 expression was significantly upregulated in cancer regions of HCC compared with adjacent regions. Increased SNHG17 expression level was correlated with tumor size, TNM stage and poor survival prognosis in HCC patients. Further functional experiments indicated that inhibition of SNHG17 significantly inhibited HCC cell proliferation, migration and invasion, caused cell cycle G0/G1 phase arrest and apoptosis. CONCLUSIONS: In summary, our findings suggest that SNHG17 might function as novel therapeutic target for the treatment of HCC.

13.
Zhonghua Bing Li Xue Za Zhi ; 50(6): 645-649, 2021 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-34078054

RESUMO

Objective: To identify important prognostic molecular markers of triple negative breast cancer (TNBC) using high throughput sequencing technology and to explore the correlation of spindle checkpoint protein BUB1B and clinicopathological features with patients' prognosis. Methods: The clinicopathological data and prognostic information of TNBC diagnosed at the West China Hospital of Sichuan University from 2009 to 2017 were collected. Forty-seven fresh tumor samples and 139 formalin fixed paraffin-embedded samples were selected. The fresh tumor samples were subject to RNA sequencing (RNA-seq). The enrichment analysis and protein-protein interaction (PPI) analysis were performed after intersection of difference analysis between RNAseq and GEO (Gene Expression Omnibus) datasets GSE38959 and GSE65194. Kaplan-Meier plotter database was used to analyze the relationship between expression of BUB1B and prognosis. Immunohistochemical staining was used to verify its expression in TNBC and correlation with clinicopathological features and prognosis. Results: Using edgeR to perform differential expression analysis between 47 TNBC tumor tissues and 12 normal tissues, 1 559 up-regulated genes and 1 376 down-regulated genes were identified, while only 131 differentially expressed genes were overlapping with those in GSE38959 and GSE65194. Enrichment analysis was mainly enriched in cell cycle, JAK-STAT signaling pathway and p53 signaling pathway. The top 10 genes ranked by degree of association were TOP2A, BUB1B, MKI67, PLK1, RRM2, PCNA, KPNA2, SMC4, PBK and IGF1. Kaplan-Meier plotter database analysis showed that the expression of BUB1B was significantly correlated with the prognosis of TNBC [overall survival, hazard ratio (HR)=0.52, 95%CI (0.35-0.77), P=0.001; distant metastasis-free, HR=0.72, 95%CI (0.52-0.98), P=0.038]. The immunohistochemical analyses of 139 formalin fixed paraffin-embedded samples showed that the low expression of BUB1B was correlated with poor prognosis in TNBC [HR=0.41, 95%CI (0.18-0.95), P=0.024]. Conclusions: The low expression of BUB1B protein is associated with poor prognosis in TNBC patients, and the molecular mechanism related with prognosis and potential therapeutic targets need to be further studied.


Assuntos
Neoplasias de Mama Triplo Negativas , Proteínas de Ciclo Celular/genética , China , Regulação Neoplásica da Expressão Gênica , Humanos , Estimativa de Kaplan-Meier , Prognóstico , Proteínas Serina-Treonina Quinases/genética , Neoplasias de Mama Triplo Negativas/genética
15.
Zhonghua Yi Shi Za Zhi ; 51(2): 103-110, 2021 Mar 28.
Artigo em Chinês | MEDLINE | ID: mdl-34098703

RESUMO

The quarantine and treatment plays an important role in prevention and control of respiratory infectious diseases. It has been nearly 200 years since sanatorium model was adopted to isolate and treat patients with tuberculosis(TB). The era of the Republic of China was an important period for the model to sprout and expand. TB sanatorium was established in the early 20th century in China, and flourished in the 1930s. Establishment and changes of TB sanatoriums in Peking, Shanghai and Hangzhou during the era of the Republic of China, has great significance for sorting out the clues of China's anti-TB history, exploring historical trends of TB in China, and most important of all, summing up valuable lessons.


Assuntos
Tuberculose , Pequim , China , Hospitais , Humanos , Taiwan
16.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(6): 539-548, 2021 Jun 09.
Artigo em Chinês | MEDLINE | ID: mdl-34098669

RESUMO

Objective: To study whether high-fat diet could aggravate the effect of periodontitis on gut microbiota and glucose metabolism. Methods: Twenty-four male SD rats were randomly and equally divided into four groups based on table of random numbers (n=6 in each group): control group, in which rats were given normal chow diet; periodontitis group, in which periodontitis was induced by ligating bilateral maxillary second molars with 5-0 silk thread; high-fat diet group, in which rats were given high-fat diet; high-fat diet+periodontitis group, in which rats were given high-fat diet and periodontitis was induced at the end of the 8th week. Fasting blood glucose and glucose tolerance were measured at the end of the 12th week. Then the rats were euthanized and the cecum content was collected. The microbial 16S rRNA gene sequencing was performed on the Illumina MiSeq platform. The taxonomy of the sequences was analyzed through RDP Classifier (http://rdp.cme.msu.edu/) against the SILVA (SSU123) 16S rRNA database. Pearson correlation analysis was performed to analyze the correlation between changes in gut microbiota and blood glucose. Results: After 4 weeks of periodontitis induction, the fasting blood glucose levels of the periodontitis group and the high-fat diet group were (4.93±0.28) and (5.25±0.24) mmol/L, respectively, which were significantly higher than that of the control group [(4.56±0.20) mmol/L] (P<0.05) with glucose intolerance. The fasting blood glucose level of high-fat diet+periodontitis group [(5.53±0.14) mmol/L] was significantly higher than that of periodontitis group and high-fat diet group, respectively (P<0.05), with the glucose tolerance curve higher than that of periodontitis group. The 16S rRNA gene analysis revealed that the Bacteroides/Firmicutes ratio in the periodontitis group is (0.37±0.23), which was significantly lower than that of the control group (0.68±0.05) (P<0.05). The relative abundance of Lachnospiraceae_NK4A136_group in the periodontitis group was (14.03±6.38)%, which was significantly lower than that of the control group [(28.21±4.82)%] (P<0.05). The relative abundance of Allobaculum [(4.27±2.67)%], Ruminococcaceae_UCG_005 [(3.70±0.90)%], Blautia [(0.63±0.45)%] in the periodontitis group were significantly higher than those of the control group [(0.60±0.72) %, (0.43±0.16) %, (0.13±0.13) %, respectively](P<0.05). Compared with periodontitis group, the relative abundance of Proteobacteria in high-fat diet+periodontitis group [(3.06±0.90)%] was significantly higher than that of the periodontitis group [(1.40±0.98)%] (P<0.05). The principal coordinate analysis and similarity analysis based on the Bray-Curtis distance showed that samples of the high-fat diet+periodontitis group clustered separately from the periodontitis group and the high-fat diet group. The results of correlation analysis showed that the abundance of Lachnospiraceae_NK4A136_group was negatively correlated with fasting blood glucose and glucose levels after loading for 60 and 120 minutes (r=-0.56, -0.50, -0.42, respectively) (P<0.05). The abundance of Allobaculum, [Eubacterium]_coprostanoligenes_group, Peptococcaceae_uncultured, [Ruminococcus]_torques_group, and several genera belonging to the Proteobacteria were positively correlated with glucose levels after loading for 120 minutes (P<0.05). Conclusions: Periodontitis might be closely related to impaired gut microbiota and glucose metabolism, and the effect could be aggravated by high-fat diet.


Assuntos
Microbioma Gastrointestinal , Periodontite , Animais , Dieta Hiperlipídica , Masculino , RNA Ribossômico 16S/genética , Ratos , Ratos Sprague-Dawley
17.
Zhonghua Yan Ke Za Zhi ; 57(6): 426-432, 2021 Jun 11.
Artigo em Chinês | MEDLINE | ID: mdl-34098691

RESUMO

Objective: To identify factors associated with persistent subretinal fluid (SRF) after scleral buckling and to evaluate surgical outcomes of patients with primary rhegmatogenous retinal detachment (RRD). Methods: Cross-sectional study and cohort study. This study included 104 patients (104 eyes) who underwent scleral buckling for repair of RRD between January 2016 and June 2017. Several statistically significant risk factors associated with SRF were screened out with univariate analysis. Then independent risk factors were determined with multivariate stepwise logistic regression analysis. Examinations were taken preoperatively, at 1, 3, 6, and 12 months postoperatively, and thereafter every six months. Patients were divided into two groups depending on the presence or absence of persistent SRF on optical coherence tomography at 1 month. Results: Persistent SRF occurred in 69.2% (72/104) of patients. In multivariate analysis, younger age (OR=32.860, 95%CI=3.636-296.986, P=0.002), high myopia (OR=7.229, 95%CI=1.217-42.94, P=0.03) and macula-involving retinal detachment (OR=357.397, 95%CI=29.761-4292.0, P=0.000) were associated with persistent SRF. Best corrected visual acuity in patients with SRF at 1 month (0.71±0.18 vs. 0.58±0.11; t=4.047, P<0.01) and 3 months (0.55±0.15 vs. 0.43±0.12; t=3.914, P<0.01) was worse than that in patients with absence of SRF. Best corrected visual acuity in patients with SRF was 0.44±0.16 at 6 months, 0.37±0.12 at 12 months and 0.36±0.10 at the last follow-up, with no significant difference from patients without SRF at the three time points (0.39±0.13, 0.38±0.12 and 0.35±0.09; t=1.643, -0.202, 0.464; P>0.05). Conclusions: RRD patients with younger age, high myopia and macular involvement were more likely to develop persistent SRF after scleral buckling. The presence of persistent SRF slowed visual recovery but did not influence the final visual outcomes. (Chin J Ophthalmol, 2021, 57: 426-432).


Assuntos
Descolamento Retiniano , Líquido Sub-Retiniano , Estudos de Coortes , Estudos Transversais , Humanos , Modelos Logísticos , Descolamento Retiniano/cirurgia , Estudos Retrospectivos , Fatores de Risco , Recurvamento da Esclera , Tomografia de Coerência Óptica , Acuidade Visual
18.
Drugs Today (Barc) ; 57(6): 377-385, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34151904

RESUMO

Small cell lung cancer (SCLC) is a rapidly progressive, aggressive metastatic and lethal subtype of lung cancer. Unfortunately, there has been little progress regarding the development of novel treatments for SCLC. However, lurbinectedin, a transcriptional inhibitor, has emerged as a potential novel treatment for cancer. It produces antitumor efficacy by inhibiting oncogenic transcription activity, inducing the accumulation of DNA double-strand breaks and modulating the tumor microenvironment (TME). Data from phase I/II trials indicates that lurbinectedin has significant antitumor efficacy and tolerable adverse effects in SCLC patients. Furthermore, lurbinectedin is efficacious in platinum-sensitive and platinum-resistant SCLC patients and in those with SCLC relapse after second-line treatment. In 2020, the U.S. Food and Drug Administration (FDA) approved lurbinectedin for the treatment of adult patients with metastatic SCLC or for patients that have received platinum-based chemotherapy. In this review, we discuss the molecular profile and the preclinical and clinical studies of lurbinectedin in the treatment of SCLC patients.


Assuntos
Neoplasias Pulmonares , Carcinoma de Pequenas Células do Pulmão , Adulto , Carbolinas , Compostos Heterocíclicos de 4 ou mais Anéis , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Recidiva Local de Neoplasia , Carcinoma de Pequenas Células do Pulmão/tratamento farmacológico , Microambiente Tumoral
19.
Fa Yi Xue Za Zhi ; 37(2): 175-180, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34142477

RESUMO

Abstract: Objective To establish the basic data for estimating minimum postmortem interval (PMImin) of heavily decayed and skeletonized remains by studying the development of Dermestes maculatus DeGeer (Coleoptera: Dermestidae). Methods The developmental stages of Dermestes maculatus were observed at four constant temperatures of 20 ℃, 24 ℃, 28 ℃ and 32 ℃, and the changes in body length were also examined as the biological indicator to estimate larval day-age and instar. Results The total developmental time from egg to adult at 20 ℃, 24 ℃, 28 ℃ and 32 ℃ were (126.7±10.6) d, (69.4±8.2) d, (50.4±8.4) d and (49.6±6.5) d, respectively. The body length increased gradually, but changed irregularly as a whole. Conclusion The study provides basic data on the development and growth of Dermestes maculatus, especially on its developmental duration as a significant value for estimating PMImin of heavily decayed and skeletonized remains. Nevertheless, the change of body length is not found to be the best biological indicator for instar determination.


Assuntos
Besouros , Animais , Autopsia , Larva , Temperatura
20.
Anim Genet ; 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-34182604

RESUMO

Breeding for good meat quality performance while maintaining large body size and desirable carcass traits has been the major challenge for modern swine selective breeding. To address this goal, in the present work we studied five related populations produced by two commercial breeds (Berkshire and Duroc) and two Chinese breeds (Licha black pig and Lulai black pig). A single-trait GWAS performed on 20 body size and carcass traits using a self-developed China Chip-1 porcine SNP50K BeadChip identified 11 genome-wide significant QTL on nine chromosomes and 22 suggestive QTL on 15 chromosomes. For the 11 genome-wide significant QTL, eight were detected in at least two populations, and the rest were population-specific and only mapped in Shanxia black pig. Most of the genome-wide significant QTL were pleiotropic; for example, the QTL around 75.65 Mb on SSC4 was associated with four traits at genome-wide significance level. After screening the genes within 50 kb of the top SNP for each genome-wide significant QTL, NR6A1 and VRTN were chosen as candidate genes for vertebrae number; PLAG1 and BMP2 were identified as candidate genes for body size; and MC4R was the strong candidate gene for body weight. The four genes have been reported as candidates for thoracic vertebrae number, lumbar vertebrae number, carcass length and body weight respectively in previous studies. The effects of VRTN on thoracic vertebrae number, carcass length and body length have been verified in Shanxia black pig. Therefore, the VRTN genotype could be used in gene-assisted selection, and this could accelerate genetic improvement of body size and carcass traits in Shanxia black pig.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...