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2.
Mol Med Rep ; 21(2): 918-926, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31974623

RESUMO

Isodicentric Y chromosomes are considered one of the most common structural abnormalities of the Y chromosome. Neocentric marker chromosomes, with neocentromeres, have drawn increasing attention in recent years. The present study reported an azoospermic male with a neocentric isochromosome Yp, neo(Yp), and an isodicentric Yq, idic(Yq). The karyotype was analyzed using G­banding, chromosome microarray analysis (CMA), and fluorescence in situ hybridization (FISH) with various detection probes, including sex­determining region on the Y chromosome (SRY) and Y centromeric, applied at the same time. G­banding initially revealed the karyotype 47,X,i(Y)(q10),+mar. CMA indicated the presence of an extra Y chromosome, seemingly equivalent to 47,XYY males. FISH delineated the existence of two centromeres on the idic(Yq). For the marker chromosome, two SRY signals were detected instead of the Y­specific centromere signal, and a visual centromere was observed. This indicated the possible existence of a neocentromere in the marker chromosome, located in the connected region in Yp11.2 band. Finally, the patient's karyotype was established as 47,X,idic(Y)(p11.2), neo(Y)(pter→Yp11.2::Yp11.2→pter). The findings suggested that both idic(Yq) and neo(Yp) could be the main causes of the patient's azoospermia, despite the fact that the partial disomy of Ypter to Yp11.2 did not lead to any major malformations. The present study not only improves the understanding of karyotype/phenotype relationships between neocentric marker Y chromosomes and male infertility, but also supports the hypothesis that the combined application of molecular cytogenetic analysis could aid in reliably confirming breakpoints, origins, and the constitution of the marker chromosomes.

3.
Medicine (Baltimore) ; 99(1): e18695, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895838

RESUMO

RATIONALE: Jacobsen syndrome (JBS) is a rare chromosomal disorder with variable phenotypic expressivity, which is usually diagnosed in infancy and childhood based on clinical examination and hematological and cytogenetic findings. Prenatal diagnosis and fetal ultrasonographic findings of JBS are rare. PATIENT CONCERNS: A 38-year-old, gravida 3, para 1, pregnant woman underwent clinical ultrasound examination at 22 weeks of gestation. DIAGNOSES: Ultrasonographic findings indicated an interventricular septal defect, the presence of septal blood flow, dilation of the left renal pelvis, and a single umbilical artery. Amniocentesis was performed to evaluate possible genetic causes of this diagnosis by cytogenetic and single nucleotide polymorphism (SNP) array analysis. INTERVENTIONS: After genetic counseling and informed consent, the couple elected to terminate the pregnancy. OUTCOMES: Karyotype analysis showed that the fetal karyotype was 46,XX,del(11)(q23). The SNP array revealed a 6.118 Mb duplication of 11q23.2q23.3 and a 15.03 Mb deletion of 11q23.3q25. LESSONS: Ultrasonographic findings of fetal JBS, including an interventricular septal defect, dilation of the left renal pelvis, and a single umbilical artery, may be associated with a 15.03 Mb deletion of 11q23.3q25. Further cases correlating phenotype and genotype are required to predict the postnatal phenotype.


Assuntos
Síndrome da Deleção Distal 11q de Jacobsen/diagnóstico por imagem , Adulto , Feminino , Humanos , Cariótipo , Gravidez , Ultrassonografia Pré-Natal
4.
Sci Total Environ ; 707: 136046, 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-31863974

RESUMO

Deep-sea Bathymodiolus mussels are ubiquitous in most cold seeps and hydrothermal fields, where they have adapted to various toxic environments including high metal exposure. However, there is scarce knowledge of metal accumulation and metal-related biomarkers in B. mussels. Here, we present data for metal concentrations (Ag, Cd, Cr, Cu, Fe, Mn, Pb, and Zn) and metal related biomarkers (superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase-GPX, glutathione-GSH, metallothioneins-MTs, and lipid peroxidation-LPO) in different tissues of B. mussels from four different deep-sea geochemical settings: one cold seep and three vent fields in the West Pacific Ocean. Results showed that mussel gills generally exhibited higher metal enrichment than the mantle. Mussels from hydrothermal vents usually had higher metal concentrations (Fe, Cr, Cd, and Pb) than those from cold seep, which could be related to their higher contents in fluids or sediments. However, despite quite different metals loads among the geochemical environment settings, Mn, Zn, and Cu concentrations varied over a smaller range across the sampling sites, implying biological regulation by deep-sea mussels for these elements. Several statistically significant correlations were observed between SOD, CAT, GSH, MTs, and metal levels in analyzed tissues. Although the vent ecosystem is harsher than the cold seep ecosystem, according to our results their mussels' biomarker levels were not so different. This finding suggests that some adaptive or compensatory mechanisms may occur in chronically polluted deep-sea mussels. Principal component analysis allowed for distinguishing different deep-sea settings, indicating that B. mussels are robust indicators of their living environments. We also compared our results with those reported for coastal mussels. To our best knowledge, this is the first integrated study to report metal accumulation and metal-related biomarkers in the deep-sea B. mussels from the West Pacific.

5.
Dalton Trans ; 49(3): 651-658, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31844872

RESUMO

A novel 3D coordination polymer {[Cu4.5 (BTZE)1.5 (µ3-OH)3(µ-OH)(SO4)(H2O)1.5·4H2O]}n (1) was synthesized by a solvothermal reaction of 1,2-bis(tetrazol-5-yl) ethane (BTZE) with copper sulfate. Compound (1) contained triangular [Cu3(µ3-OH)] cluster based magnetic Δ-chains linked with in situ generated µ2-BTZE ligands to form a 2D cyclic annular layer. This 2D layer structure was further modified with sulfate and symmetry-related µ3-OH groups, extending to a 3D coordination framework structure. The magnetic performance of (1) was characterized in the temperature range of 2-300 K in terms of direct-current and alternating-current magnetic susceptibilities, revealing that (1) was a canted ferromagnet with a critical temperature (Tc) of 9.5 K. Notably, (1) behaved as a hard magnet with a coercive field of 2.3 kOe at 2 K, showing significant unique characteristics compared to those of the reported spin canting systems based on pure Cu(ii) ions.

6.
Oncol Lett ; 18(6): 6877-6884, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31807191

RESUMO

The aim of the present study was to examine the association between the migration of breast cancer cells in vitro and radiosensitivity by establishing a breast cancer cell model with different migratory capacities. Transwell chambers in a 24-well plate were used to separate MDA-MB-231 and ZR-7530 cells and to establish cell models with different migratory capacities. Subsequently, the radiosensitivity of the cell models was measured using a radiation clone formation assay. Furthermore, differential gene expression was determined using gene microarray analysis. The protein expression levels of the differentially expressed genes (DEGs) were assessed using western blot analysis. From each parental cell line, a pair of daughter cell lines were established in with differing migratory abilities. These daughter cell lines were named MDA-MB-231 UP-10 (231 UP-10), MDA-MB-231 Down-10 (231 Down-10), ZR-75-30 UP-10 (7530 UP-10) and ZR-75-30 Down-10 (7530 Down-10). Radiation clone formation assays revealed that the cell lines with increased migratory abilities (231 Down-10 and 7530 Down-10) demonstrated higher radio-resistance compared with the cell lines with decreased migratory abilities (231 UP-10 and 7530 UP-10). Gene microarrays identified numerous DEGs between the pairs of UP and Down cell lines. A focus was placed on genes associated with cell adhesion and it was identified that phosphorylated Fak and phosphorylated EGFR expression levels were increased in 231 Down-10 and 7530 Down-10 cells, compared with the 231 UP-10 and 7530 UP-10 cells. Other genes including ZO-1, FN1 and SOX9 expression were also increased in the 231 Down-10 and 7530 Down-10 cells compared with 231 UP-10 and 7530 UP-10 cells. Cell lines with increased migratory capacities may be more radio-resistant compared with cell lines with a decreased migratory capabilities. The mechanism may be associated with changes in the expression of cell adhesion molecules and epithelial-mesenchymal transition (EMT). Therapeutic strategies targeting cell adhesion or EMT may increase the radiation sensitivity of breast cancer cells, in addition to improving the effect of radiation therapy.

7.
Medicine (Baltimore) ; 98(47): e18041, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31764825

RESUMO

Fetal chromosomal abnormalities are considered to be the main cause of spontaneous abortion (SA). We aimed to determine the differences in the rates and numbers of chromosomal abnormalities between samples from women with a history of one versus more than one SA as well as between samples from first- and second-trimester SAs in women from Northeast China.In total, 1210 products of conception (POCs) from patients with a history of one or more SAs were examined. Of these 1210 samples, 434 were from women with a history of 1 SA, and 776 were from women with a history of more than 1 SA. Additionally, 1071 samples were from the first trimester, 118 were from the second trimester, and 21 were from the third trimester. We identified chromosomal abnormalities by next-generation sequencing (NGS) technology. Among the 1210 POCs in women with SA, 607 (50.17%) had fetal chromosomal abnormalities. There were no significant differences in the rates of chromosomal abnormalities according to the abortion frequency. However, first-trimester SA had a significantly higher percentage of fetal chromosomal abnormalities than second-trimester SA (P < .05). Among 663 chromosomal abnormalities, 633 abnormalities occurred in first-trimester SA; the most frequent karyotype was trisomy 16 (14.38%), followed by monosomy X (13.27%), trisomy 22 (7.90%), and trisomy 15 (5.37%). Thirty abnormalities occurred in second-trimester SA; the most frequent karyotype was trisomy 18 (26.67%), followed by monosomy X (16.67%), trisomy 21 (13.33%), and trisomy 13 (10.00%). No chromosomal abnormalities occurred in the third trimester.These findings indicate the importance of determining the genetic cause of abortion in patients with a history of SA. We also identified a trend suggesting that the percentage of fetal chromosomal abnormalities is significantly higher in first- than second-trimester SA. The detection rate of chromosomal abnormalities in POCs from SA can be increased by NGS, which is beneficial for couples with recurrent miscarriages and offers better genetic counseling in the clinical setting.


Assuntos
Aborto Espontâneo/genética , Aberrações Cromossômicas , Variações do Número de Cópias de DNA , Feto , Sequenciamento de Nucleotídeos em Larga Escala , Feminino , Humanos , Gravidez
8.
J Exp Bot ; 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31677347

RESUMO

Neighbor detection and allelochemical response are important mediators in plant-plant interactions. Despite increasing knowledge of plant allelochemicals in response to the presence of competitors involved in the neighbor-derived signaling chemicals, less is known about which signaling chemicals are responsible for the neighbor-induced allelochemical response. Here, we experimentally demonstrated (-)-loliolide, a carotenoid metabolite, as a signaling chemical in barnyardgrass-rice allelopathic interactions. The production of rice allelochemicals momilactone B and tricin was increased in the presence of five biotypes of barnyardgrass. (-)-Loliolide was found in all biotypes of barnyardgrass and their root exudates and rhizosphere soils. There were significant positive relationships between rice allelechemicals and (-)-loliolide concentrations across biotypes of barnyardgrass. Furthermore, (-)-loliolide elicited the production of momilactone B and tricin. Comparative transcriptome analysis showed regulation of (-)-loliolide on diterpenoid and flavonoid biosynthesis pathway. The expression of key genes involved in the biosynthesis of momilactone B (CPS4, KSL4 and MAS) and tricin (CYP75B3 and CYP75B4) was up-regulated by (-)-loliolide. These findings suggest that (-)-loliolide as a signaling chemical participates in barnyardgrass-rice allelopathic interactions. Through the signaling chemical, allelopathic rice plants can detect competing barnyardgrass and respond by increasing allelochemical levels to provide an advantage for their own growth.

9.
Mol Cytogenet ; 12: 44, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31700544

RESUMO

Background: 46,XX male syndrome is a rare disorder that usually causes infertility. This study was established to identify the genetic causes of this condition in a series of 46,XX males through the combined application of cytogenetic and molecular genetic techniques. Case presentation: We identified eight azoospermic 46,XX males who underwent infertility-related consultations at our center. They all presented normal male phenotypes. In seven of the eight 46,XX males (87.5%), translocation of the SRY gene to the terminal short arm of the X chromosome was clearly involved in their condition, which illustrated that this translocation is the main mechanism of 46,XX sex reversal, in line with previous reports. However, one patient presented a homozygous DAX1 mutation (c.498G > A, p.R166R), which was not previously reported in SRY-negative XX males. Conclusions: We proposed that this synonymous DAX1 mutation in case 8 might not be associated with the activation of the male sex-determining pathway, and the male phenotype in this case might be regulated by some unidentified genetic or environmental factors. Hence, the detection of genetic variations associated with sex reversal in critical sex-determining genes should be recommended for SRY-negative XX males. Only after comprehensive cytogenetic and molecular genetic analyses can genetic counseling be offered to 46,XX males.

10.
Medicine (Baltimore) ; 98(41): e17407, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31593094

RESUMO

BACKGROUND: To evaluate the safety of intracytoplasmic sperm injection (ICSI) for men with Y chromosome azoospermia factor (AZF) microdeletions. METHODS: Twenty-five men with Y chromosome microdeletions and their partners underwent ICSI treatment. These subjects were matched against 50 ICSI cycles in which the patients had normal Y chromosomes. RESULTS: Among the 25 couples, 17 achieved a clinical pregnancy of which 14 continued to a live birth. Sixteen men had deletions of AZFc markers (sY152, sY254, and sY255), 1 had a deletion of sY152, 3 had a deletion of sY254, sY255, 1 had a deletion of sY152, sY239, Sy242, sY254, and sY255, and 3 had deletions of sY152, sY254, sY255, and sY157. AZFb microdeletions (sY127, sY134, and sY143) were found in 1 patient. AZF microdeletions had no adverse effects on the clinical pregnancy, implantation or delivery rates, birth weight, gestational age, or sex ratio when compared with the control group. Overall, the multiple gestation and preterm delivery rates of the AZF microdeletion group were similar to those in the control group. CONCLUSION: Men with AZF microdeletions can achieve the delivery of healthy children using ICSI. In this series, it produced good implantation rate and obstetric and perinatal outcomes.


Assuntos
Azoospermia/terapia , Infertilidade Masculina/terapia , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/terapia , Injeções de Esperma Intracitoplásmicas/métodos , Adulto , Azoospermia/genética , Deleção Cromossômica , Cromossomos Humanos Y/genética , Feminino , Humanos , Recém-Nascido , Infertilidade Masculina/genética , Masculino , Gravidez , Resultado da Gravidez , Aberrações dos Cromossomos Sexuais , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética , Resultado do Tratamento
11.
Carcinogenesis ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31504235

RESUMO

N6-methyladenosin (m6A) is one of widespread post-transcriptional mRNA modifications in eukaryotes and the m6A modification plays critical roles in various human cancers. However, the role of m6A-binding proteins in cancer metabolism remains elusive. Here we report that YTH domain family 2 (YTHDF2) is upregulated in lung cancer tissues, promotes lung cancer cell growth, and enhances the pentose phosphate pathway (PPP) flux, which is crucial for tumor growth. Mechanistically, YTHDF2 directly binds to the m6A modification site of 6-phosphogluconate dehydrogenase (6PGD) three prime untranslated region (3'-UTR) to promote 6PGD mRNA translation in lung cancer cells. Collectively, our data indicate that YTHDF2 acts as a tumor promoter to enhance tumor growth via facilitating 6PGD mRNA translation.

12.
J Exp Clin Cancer Res ; 38(1): 388, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31488193

RESUMO

BACKGROUND: Hypoxia is a key feature of breast cancer, which affects cancer development, metastasis and metabolism. Previous studies suggested that circular RNAs (circRNAs) could participate in cancer progression and hypoxia regulation. This study aimed to investigate the role of circRNA differentially expressed in normal cells and neoplasia domain containing 4C (circDENND4C) in breast cancer progression under hypoxia. METHODS: Forty-three patients with breast cancer were involved in this study. Breast cancer cell lines MDA-MB-453 and SK-BR-3 were cultured under hypoxia (1% O2) for experiments in vitro. The expression levels of circDENND4C, microRNA-200b (miR-200b) and miR-200c were measured by quantitative real-time polymerase chain reaction. Glycolysis was investigated by glucose consumption, lactate production and hexokinase II (HK2) protein level. Migration and invasion were evaluated via trans-well assay and protein levels of matrix metallopeptidase 9 (MMP9) and MMP2. The interaction between circDENND4C and miR-200b or miR-200c was explored by bioinformatics analysis, luciferase assay and RNA immunoprecipitation. Murine xenograft model was established to investigate the anti-cancer role of circDENND4C in vivo. RESULTS: circDENND4C highly expressed in breast cancer was up-regulated in response to hypoxia. Knockdown of circDENND4C decreased glycolysis, migration and invasion in breast cancer cells under hypoxia. circDENND4C was validated as a sponge of miR-200b and miR-200c. Deficiency of miR-200b or miR-200c reversed the suppressive effect of circDENND4C knockdown on breast cancer progression. Moreover, silence of circDENND4C reduced xenograft tumor growth by increasing miR-200b and miR-200c. CONCLUSION: circDENND4C silence suppresses glycolysis, migration and invasion in breast cancer cells under hypoxia by increasing miR-200b and miR-200c.


Assuntos
Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Fatores de Troca do Nucleotídeo Guanina/genética , Hipóxia/genética , MicroRNAs/genética , /genética , Adulto , Idoso , Animais , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células , Modelos Animais de Doenças , Feminino , Glicólise , Humanos , Hipóxia/metabolismo , Camundongos , Pessoa de Meia-Idade , Metástase Neoplásica , Estadiamento de Neoplasias
13.
Prenat Diagn ; 39(12): 1120-1126, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31461790

RESUMO

INTRODUCTION: Pure duplication of chromosome 18p is rare, with clinical phenotypes ranging from normal or slight abnormalities to various degrees of mental retardation. It remains difficult to establish a clear genotype-phenotype correlation. METHODS: Chromosomal karyotyping analysis was performed on cultured amniotic fluid cells from three cases. Single nucleotide polymorphism (SNP) array analysis was carried out using the Illumina Human CytoSNP-12 BeadChip. We also carried out a review of the literature regarding 18p11 microduplication. RESULTS: G-banding analysis showed that the three cases had normal karyotypes. SNP array results showed 0.48- to 1.6-Mb microduplications of 18p11.31-p11.22 (chr18: 6995739-8713088) in these cases, encompassing different degrees of LAMA1 duplication. Follow-up analysis showed that the parents of both cases 1 and 2 chose termination of pregnancy. Case 3 presented with normal growth and physical development. Currently, there is not enough evidence supporting the pathogenicity of LAMA1 triplosensitivity. CONCLUSION: We described three prenatal cases with 18p11.31-p11.22 microduplications involving part of the LAMA1 locus. There might be phenotypic diversity associated with 18p11.31-p11.22 microduplications. To avoid unnecessary abortions for pregnancies such as these, comprehensive genetic counseling should be offered.

14.
Med Sci Monit ; 25: 5801-5812, 2019 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-31377750

RESUMO

BACKGROUND This study aimed to screen common and low-frequency variants of nonobstructive azoospermia (NOA)-associated genes, and to construct a database for NOA-associated single nucleotide variants (SNVs). MATERIAL AND METHODS Next-generation sequencing of 466 NOA-associated genes was performed in 34 patients with NOA (mean age, 29.06±4.49 years) and 40 sperm donors (mean age, 25.08±5.75 years) from the Han population of northeast China. The SNV database was constructed by summarizing NOA non-negatively-associated SNVs showing statistical differences between NOA cases and controls, and then selecting low-frequency variants using Baylor's pipeline, to identify statistically valid SNVs. RESULTS There were 65 SNVs identified that were significantly different between both groups (p<0.05). Five genetic variants showed positive correlations with NOA: MTRR c.537T>C (rs161870), odds ratios (OR), 3.686, 95% confidence interval (CI), 1.228-11.066; MTRR, c.1049A>G (rs162036), OR, 3.686, 95% CI, 1.228-11.066; PIWIL1, c.1580G>A (rs1106042), OR, 4.737, 95% CI, 1.314-17.072; TAF4B, c.1815T>C (rs1677016), OR, 3.599, 95% CI, 1.255-10.327; and SOX10 c.927T>C (rs139884), OR, 3.192, 95% CI, 1.220-8.353. Also, 52 NOA non-negatively associated SNVs and 39 SNVs were identified by Baylor's pipeline and selected for the SNV database. CONCLUSIONS Five genetic variants were shown to have positive correlations with NOA. The SNV database constructed contained NOA non-negatively associated SNVs and low-frequency variants. This study showed that this approach was an effective strategy to identify risk alleles of NOA.

15.
Exp Ther Med ; 18(2): 1267-1275, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31363371

RESUMO

Trisomy 16q is a rare disorder with severe abnormalities, which always leads to early postnatal mortality. It usually results from a parental translocation, exhibiting 16q duplication associated with another chromosomal deletion. The present study reports on the clinical presentation and molecular cytogenetic results of a small-for-gestational-age infant, consisting of partial trisomy 16q21→qter and monosomy 2p25.3→pter. The proband presented with moderately low birthweight, small anterior fontanelles, prominent forehead, low hairline, telecanthus, flat nasal bridge, choanal atresia, clinodactyly of the fifth fingers, urogenital anomalies, congenital muscular torticollis and congenital laryngomalacia. The last two traits have not previously been reported in any trisomy 16q and monosomy 2p cases. The proband was trisomic for the 16q21→qter chromosomal region with the karyotype 46,XY,der(2)t(2;16)(p25;q21)pat. The chromosomal anomaly was the result of unbalanced segregation of a paternal balanced translocation, 46,XY,t(2;16)(p25;q21). In this case, molecular cytogenetic analysis had a critical role in delineating the proband's clinical phenotype. Although this patient had a 16q21→qter duplication and a 2p25.3→pter deletion, the latter may have had mild phenotypic effects when associated with trisomy 16q. The literature was also reviewed, focusing on cases with the same breakpoints, localizations and clinical features reported in recent years.

16.
Medicine (Baltimore) ; 98(26): e16209, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31261571

RESUMO

It is not clear whether age has any influence on the outcomes for sperm used for assisted reproductive technology in cryptozoospermic men. We evaluated intracytoplasmic sperm injection (ICSI) outcomes using ejaculated or testicular sperm in men with cryptozoospermia from different paternal age ranges.We conducted a retrospective observational study of 35 men with cryptozoospermia who underwent ICSI from 2010 to 2018. They were classified into 2 groups based on male age, namely < 35 years and ≥ 35 years. Each group was further divided into 2 subgroups according to the origin of sperm (ejaculated or testicular).In the <35 years group, the normal fertilization and high-quality embryo rates for ejaculated sperm were significantly higher than with testicular sperm (74.7% vs. 62.4%, P = .02; 50.5% vs. 36.6%, P = .03, respectively). However, in the ≥35 years group, the high-quality embryo and clinical pregnancy rates were significantly lower in the ejaculated sperm subgroup than in the testicular sperm subgroup (26.2% vs. 63%, P = .002; 12.5% vs. 71.4%, P = .04, respectively).This study indicates that ICSI should be performed as soon as possible for men with cryptozoospermia. When the paternal age ≥35 years, testicular sperm should be used for ICSI, as this offers better high-quality embryo and clinical pregnancy rates.


Assuntos
Oligospermia , Idade Paterna , Injeções de Esperma Intracitoplásmicas , Adulto , Ejaculação , Feminino , Humanos , Masculino , Gravidez , Taxa de Gravidez , Estudos Retrospectivos , Recuperação Espermática , Testículo
17.
Sci Total Environ ; 680: 44-50, 2019 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-31100667

RESUMO

Sand burial plays important role in the life history of species in sandy areas of arid and semi-arid temperate regions, by affecting seed germination, seedling growth and survival and plant community. However, few studies have focused on the effects of sand burial on the dynamics of seed dormancy in such areas. In this study, seed germination characteristics of Allium tenuissimum, a dominant perennial herb in the dune ecosystem in Ordos Plateau in northern China, was investigated. Dormancy dynamics were monitored for seeds buried at sand depths of 0, 2 and 5 cm in the natural habitats for 13 months. Freshly matured seeds of A. tenuissimum were conditionally dormant and germinated to high percentages at high but not low temperatures. Germination percentages were increased by incubation at summer temperature (15/25 °C) and dry storage, but GA3 and cold stratification had no significant effects. These results suggested that seeds of A. tenuissimum were conditionally dormant, and incubation under warm, wet (but not cold, wet) conditions and dry after-ripening resulted in complete dormancy releasing. Seeds buried at 2 and 5 cm in the field for 13 months exhibited seasonal changes in germination, with an increase in spring and summer followed by decrease in autumn and winter. However, seeds on the soil surface (0 cm) gradually came out of dormancy beginning in January, and germinated percentage was highest in July, after which remained high. The peak of nondormancy for seeds buried in the field was in the summer, which corresponds to the period of highest annual precipitation. Sand burial helped mediate seeds dormancy dynamics of A. tenuissimum via seasonal dormancy cycling. Our finding contributes to a better understanding of how timing of seedling establishment is controlled in a semiarid dune ecosystem in temperate climate.


Assuntos
Clima , Ecossistema , Germinação/fisiologia , Plantas , China , Monitoramento Ambiental , Sementes
18.
Eur J Med Chem ; 176: 135-148, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31102934

RESUMO

Angiogenesis plays an essential role in tumourigenesis and tumour progression, and anti-angiogenesis therapies have shown promising antitumour effects in solid tumours. 2-Methoxyestradiol (2ME2), an endogenous metabolite of estradiol, has been regarded as a potential antitumour agent mainly targeting angiogenesis. Here we synthesized a novel series of chalcones based on 2-methoxyestradiol and evaluated their potential activities against tumours. Compound 11e was demonstrated to have potent antiangiogenic activity. Further studies showed that 11e suppressed tumour growth in human breast cancer (MCF-7) xenograft models without obvious side effects. Evaluation of the mechanism revealed that 11e targeted the epithelial to mesenchymal transition (EMT) process in MCF-7 cells and inhibited HUVEC migration and then contributed to hindrance of angiogenesis. Thus, 11e may be a promising antitumour agent with excellent efficacy and low toxicity.


Assuntos
2-Metoxiestradiol/análogos & derivados , 2-Metoxiestradiol/uso terapêutico , Inibidores da Angiogênese/uso terapêutico , Antineoplásicos/uso terapêutico , Chalconas/uso terapêutico , Transição Epitelial-Mesenquimal/efeitos dos fármacos , 2-Metoxiestradiol/síntese química , 2-Metoxiestradiol/toxicidade , Inibidores da Angiogênese/síntese química , Inibidores da Angiogênese/química , Inibidores da Angiogênese/toxicidade , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Antineoplásicos/toxicidade , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Chalconas/síntese química , Chalconas/química , Chalconas/toxicidade , Galinhas , Membrana Corioalantoide/efeitos dos fármacos , Feminino , Células Endoteliais da Veia Umbilical Humana , Humanos , Masculino , Camundongos Nus , Receptores de Fatores de Crescimento do Endotélio Vascular/antagonistas & inibidores , Estereoisomerismo , Ensaios Antitumorais Modelo de Xenoenxerto
19.
Front Pharmacol ; 10: 356, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31024318

RESUMO

Copper chaperone for superoxide dismutase (CCS) is a critical component of oxidation-reduction system and functions as a potential tumor promoter in several cancers. However, the function and clinical significance of CCS in breast cancer remain unclear. Here, we found CCS was highly expressed in breast cancer, where it promoted breast cancer cell proliferation and migration. Suppression of CCS expression was sufficient to attenuate the phosphorylation level of ERK1/2 and increase the accumulation of reactive oxygen species (ROS). Mechanistically, we found that knockdown of CCS decreases the activity of ERK1/2 mediated by the accumulation of ROS, which leads to the inhibition of cell proliferation and migration. In summary, these results indicated that CCS promotes the growth and migration of breast cancer cells via regulating the ERK1/2 activity mediated by ROS.

20.
Disaster Med Public Health Prep ; 13(4): 695-699, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30832750

RESUMO

OBJECTIVE: Mine rescue teams bear a high risk of injury. To improve medical emergency preparedness and injury prevention, this work analyzed the causes and severity of mine rescue teams' casualty incidents, the primary injuries, and the link between the causes and the occurrences of the casualty incidents. METHODS: A total of 81 cases from 1953 to 2013 were used to analyze the casualty incidents of mine rescue teams based on the frequency of accidents. A panel with 4 rescue experts was set up to ensure the accuracy of the analysis. RESULTS: The 81 casualty incidents occurred in 7 types of rescue work and were due to 6 causes. Organizational and personal factors were the leading cause, followed by rescue skill and equipment factors. Problems with decision-making and command have gradually become the primary inducement of casualty incidents in recent years, with an average death toll reaching up to 6 to 7 people. The main injuries causing death to team members were blast injury, burns, poisoning, suffocation, blunt trauma, and overwork injury. Some of the injured died because of medical emergency response failure. CONCLUSION: The construction of emergency medical teams and the preparedness of disaster medicine need to be improved to reduce the mortality of the injured team members. Actions according to the causes of casualty incidents should be adopted for injury prevention. (Disaster Med Public Health Preparedness. 2019;13:695-699).

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