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1.
Talanta ; 207: 120256, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594590

RESUMO

Endogenous metabolites of amino acids and their derivatives in biosamples are frequently highlighted as the most differential metabolites in recent metabolomics studies. The method for the detection of amino acid derivatives such as N-acetyl amino acids and oligopeptides is rarely reported. We developed a rapid, high-throughput, sensitive and reliable quantitative method to simultaneously profile 40 underivatized amino acids and their derivatives including N-acetyl amino acids and oligopeptides in cell lines, based on ultra-high-performance liquid chromatography-electrospray tandem mass spectrometry (UHPLC- MS/MS) by using a hydrophilic interaction liquid chromatography (HILIC) column. The optimized method was successfully validated with satisfactory linearity, sensitivity, accuracy, precision, matrix effects, recovery and stability for all analytes. Only one compound (cysteine-glutathione disulfide) showed relatively low recoveries at three concentration levels (60.8-74.3%). The limit of quantification (LOQ) for most compounds was in the range of 0.6-10 ng/mL (6-100 pg on column). This method was successfully applied to the analysis of amino acids and their derivatives in breast cancer cell samples. Principal component analysis (PCA) and the orthogonal projections to latent structures (OPLS) showed a clear discrimination of the non-tumorigenic breast epithelial cell line MCF-10A from the breast cancer cell line HCC 1806. Characteristic metabolic changes in amino acid metabolism were observed in the breast cancer cell line. This quantified analytical method of 40 endogenous amino acids and their derivatives in cell lines meets the requirement of quantification in specific expanded metabolomics studies with good sensitivity.

2.
J Pharm Biomed Anal ; 177: 112848, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31479998

RESUMO

Metabolic syndrome (MetS) is an important risk factor for type 2 diabetes, cardiovascular diseases and all-cause morbidity and mortality. Biomarkers can provide insight into the mechanism, facilitate early detection, and monitor progression of MetS and its response to therapeutic interventions. To identify potential biomarkers, we applied a non-targeted and targeted lipidomics method to characterize plasma metabolic profile in MetS patients. Metabolic profiling was performed on a non-target set (40 cases and 40 controls) on UHPLC-Q-TOF/MS and target set (80 MetS patients and 80 healthy controls) on UHPLC-Q-orbitrap MS. Using comprehensive screening and validation workflow, we identified a panel of three metabolites including PC(18:1/P-16:0), PC(o-22:3/22:3), PC(P-18:1/16:1). Our results indicated that the identified biomarkers may improve the risk prediction and provide a novel tool for monitoring of the progression of disease and response to treatment in MetS patients.

3.
Food Chem ; 307: 125550, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31639575

RESUMO

Modified atmosphere has widely been evident to contribute to fruit quality maintenance, however the correlation among these quality traits was less known. To explore main factors of elevated atmosphere and reduce the detection indexes, we exposed strawberry to either high O2 (80% O2 + 20% N2) or CO2 (20% CO2 + 20% O2 + 60% N2) atmosphere and compared quality characteristics. It was demonstrated that both atmospheres well maintained the fruit firmness, alleviated weight loss and decay rate. Elevated O2 maintained the polyphenolic contents and cell integrity by significantly decreasing superoxide and hydrogen peroxide levels. PCA analysis implied that HO treatment mainly affected oxygen metabolism while HCO affected carbon metabolism more. Significantly positive correlation was observed between weight loss, anthocyanin content and decay rate in elevated O2 and control groups. This study provided new insights into correlation and difference between impact of elevated O2 and CO2 to postharvest preservation.

4.
J Sci Food Agric ; 100(1): 177-183, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31471913

RESUMO

BACKGROUND: The small and large strain rheology of gluten (G) and gluten-starch (G + S) doughs containing wheat bran dietary fiber (WBDF) were investigated. RESULTS: At the small strain stage, i.e. frequency and strain sweep tests, the doughs containing high WBDF concentration are more vulnerable and unstable, as indicated by the lower dough linear viscoelastic strain limit as well as the higher slope of elastic modulus. However, the elastic nature of doughs remarkably increased upon WBDF addition, indicating the reinforcement of the dough mechanical strength, which is also confirmed by the large strain test wherein the maximum strain significantly decreased from 4.37 to 1.82 for the G system and from 12.09 to 2.72 for the G + S system. The creep recovery test showed that WBDF induced the reduction in the strain of the doughs at a fixed stress, which may be related to the enhanced strain hardening capacity. CONCLUSION: The addition of WBDF resulted in more brittle and unstable doughs with undesirable higher mechanical strength. The presence of starch greatly weakened the dough strength and led to inferior resistance to both small and large deformations. These findings confirmed the impairment of dough viscoelasticity upon addition of WBDF. © 2019 Society of Chemical Industry.

5.
Biosens Bioelectron ; 147: 111769, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31630030

RESUMO

Rapid and accurate detection of nucleic acids plays a major role in biological research and clinical diagnostics. Here, a 3D multiple self-cleaning electrochemical ratiometric microfluidic paper-based analytical device (SER-µPAD) has been constructed for manganese super oxide dismutase (MnSOD) gene detection on the basis of the inner reference probe and exonuclease Ш (Exo Ш)-assisted analytes recycling amplification method. To simplify manual operations, a multipath self-cleaning tab that could manipulate fluid transport was introduced into the paper-based device, realizing time-programmable self-cleaning of the electrode. For achieving sensitive detection of MnSOD gene, the methylene blue (MB)-modified capture probe (CP) as the inner reference element was first self-assembled on triangular Au nanosheets modified paper working electrode to provide a built-in correction and improve the detection accuracy. When MnSOD gene existed, it hybridized with the hairpin-structured signal probe, triggering the cyclic amplification with the assistance of Exo Ш selective digestion to engender numerous residual DNA labeled with ferrocene (Fc) that could be captured on electrode surface by CPs. Hence, the Fc tags were close to the electrode surface, resulting in the oxidation peak current of Fc (IFc) increase, while that of MB (IMB) was constant on account of the unchanged distance between the MB tags and the electrode. The value of IFc/IMB was linear with MnSOD gene concentration from 10 nM to 1200 nM, and the detection limit was 3.91 nM. This strategy provides an accurate, robust, and sensitive method for nucleic acids detection and shows great potential in the construction of portable devices.

6.
Biosens Bioelectron ; 147: 111742, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31672389

RESUMO

Surface-enhanced Raman spectroscopy (SERS) as one of the effective tools for sensitive and selective detection of biomolecules has attracted tremendous attention. Here, we construct a versatile biomolecular detection platform based on photo-induced enhanced Raman spectroscopy (PIERS) effect for ultrasensitive detection of multiple analytes. In our PIERS sensor, we exploit the molecular recognition capacity of aptamers and the high affinity of aptamers with analyte to trigger TiO2@AgNP substrates binding with Raman tag-labeled gold nanoparticles probes via analyte, thus forming sandwich complexes. Additionally, combining plasmonic nanoparticles with photo-activated substrates allows PIERS sensor to achieve increased sensitivity beyond the normal SERS effect upon ultraviolet irradiation. Accordingly, the PIERS can be implemented for analysis of multiple analytes by designing different analyte aptamers, and we further demonstrate that the constructed PIERS sensor can serve as a versatile detection platform for sensitively analyzing various biomolecules including small molecules (adenosine triphosphate (ATP), limit of detection (LOD) of 0.1 nM), a biomarker (thrombin, LOD of 50 pM), and a drug (cocaine, LOD of 5 nM). Therefore, this versatile biomolecular detection platform based on PIERS effect for ultrasensitive detection of multiple analytes holds great promise to be a practical tool.

7.
Methods Mol Biol ; 2083: 3-23, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31745909

RESUMO

Carotenoids are isoprenoid compounds synthesized de novo in all photosynthetic organisms as well as in some nonphotosynthetic bacteria and fungi. In plants, carotenoids are essential for light harvesting and photoprotection. They contribute to the vivid color found in many plant organs. The cleavage of carotenoids produces small molecules (apocarotenoids) that serve as aroma compounds, as well as phytohormones and signals to affect plant growth and development. Since carotenoids provide valuable nutrition and health benefits for humans, understanding of carotenoid biosynthesis, catabolism and storage is important for biofortification of crops with improved nutritional quality. This chapter primarily introduces our current knowledge about carotenoid biosynthesis and degradation pathways as well as carotenoid storage in plants.

8.
J Cell Physiol ; 235(2): 1339-1348, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31256441

RESUMO

This study aimed to investigate the molecular mechanisms underlying the roles of metformin (MET) and Sorafenib (SOR) in the treatment of endometrial hyperplasia (EH) in polycystic ovary syndrome (PCOS). Effects of MET and SOR on the area of endometrium and myometrium were detected. Western blot analysis and immunohistochemistry assays were carried out to detect the levels of mammalian target of rapamycin complex 1 (mTORC1), mTORC2, LC3-II, P62, and Caspase-3 in rats and cultured cells. Furthermore, cell counting kit-8 assay and flow cytometry analysis was carried out to determine the apoptotic profiles of treated cells. MET and SOR could apparently decrease the areas of endometrium and myometrium in PCOS. MET notably enhanced the expression of LC3-II and Caspase-3 in PCOS while substantially reducing the level of mTORC1 and P62. Similarly, SOR also enhanced the expression of LC3-II and Caspase-3 in PCOS while substantially reducing the level of mTORC2 and P62. Treatment with MET and SOR significantly inhibited the proliferation of HCC-94 and HEC-1-A cells while promoting their apoptosis by upregulating the expression of Caspase-3. In cells treated with MET, the expression of mTORC1 and LC3-II was upregulated while the expression of P62 was downregulated. Similarly, in cells treated with SOR, the expression of mTORC2 and LC3-II was also upregulated while the expression of P62 was also downregulated. Furthermore, MET showed no effect on mTORC2 expression, while SOR showed no effect on mTORC1 expression. In this study, we suggested that MET and SOR alleviated the risk of EH in PCOS via the mTORC1/autophagy/apoptosis axis and mTORC2/autophagy/apoptosis axis, respectively.

9.
J Biomed Mater Res A ; 108(1): 30-38, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31433913

RESUMO

Niclosamide is an antihelminthic drug. Recent studies show that niclosamide exerts antitumor activity through inhibiting multiple signals including Wnt/ß-catenin, mTORC1, signal transducer and activator of transcription 3, NF-κB, notch signals; however, the insolubility and poor bioavailability limits its potential clinic use, the aim of the present work is to synthesize an injectable pegylated niclosamide (polyethylene glycol-modified niclosamide) and investigate its antitumor activity in vitro and in vivo. The pegylated niclosamide (mPEG5000-Nic) was synthesized and the chemical structure was identified by Fourier transform infrared spectra and 1 H nuclear magnetic resonance spectra. The antitumor activity was evaluated in CT26 and HCT116 colon cancer cells in vitro and nude mouse xenograft model of CT26 cells in vivo. The water solubility of niclosamide in mPEG5000-Nic was significantly increased. Niclosamide could be released from mPEG5000-Nic nanoparticles in PBS solution. mPEG5000-Nic inhibited the cell viability of CT26 and HCT116 cells in vitro. No animal death was observed in mice with intraperitoneal injection of mPEG5000-Nic (equivalent to 1000 mg/kg niclosamide) within 24 hr, indicating that mPEG5000-Nic was less toxic. In nude mouse, xenograft model of CT26 colon carcinoma, intraperitoneal injection of mPEG5000-Nic (equivalent to niclosamide 50 mg/kg) inhibited tumor growth but had no effect on animal body weight and heart, liver, kidney, and lung weight in vivo. Meanwhile, in the same model, intraperitoneal injection of the positive clinic drug 5-fluorouracil not only inhibited the tumor growth, but also reduced the animal body weight. Our study demonstrates that pegylated niclosamide is novel niclosamide delivery system with clinical perspective for cancer therapy.

10.
Int J Cancer ; 146(1): 208-222, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31251822

RESUMO

The role of Fyn-related kinase (FRK) in malignant tumors remains controversial. Our study investigated the function of FRK in lung cancer. Immunohistochemistry staining and generating a knockout of FRK by CRISPR/Cas9 in H1299 (FRK-KO-H1299) cells were strategies used to explore the role of FRK. Immunohistochemistry staining indicated that FRK expression was elevated in 223 lung cancer tissues compared to 26 distant normal lung tissues. FRK contributed to poor survival status in lung cancer patients and acted as a predictor for poor prognosis of lung cancer. Knockout of FRK by CRISPR/Cas9 markedly inhibited proliferation, invasion, colony formation and epithelial-mesenchymal transition (EMT) process in the lung cancer cell line H1299. Further exploration indicated that FRK-KO damaged the stemness phenotype of H1299 by inhibiting CD44 and CD133 expression. Seahorse detection and a U-13 C flux assay revealed that FRK-KO induced metabolism reprogramming by inhibiting the Warburg effect and changing the energy type in H1299 cells. Epidermal growth factor stimulation recovered the expression of FRK and biological functions, metabolic reprogramming and stemness phenotype of H1299 cells. FRK plays an oncogenic role in lung cancer cells via a novel regulation mechanism of enhancing the stemness of H1299 cells by inducing metabolism reprogramming, which finally promotes EMT and metastasis. Our study also indicates that FRK could be used as a potential therapeutic target for drug development.

11.
J Virol Methods ; 275: 113751, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31639372

RESUMO

The objective of this study was to develop a rapid, sensitive and specific EvaGreen (EG)-based real-time PCR assay capable of detecting Aleutian mink disease virus (AMDV) and to evaluate the reliability of the assay for analysis of blood or tissue samples. For this assay, a pair of primers was designed based on a nonstructural protein (NS)-encoding gene of AMDV, and the identity of PCR products was identified based on a melting temperature of 82.8°C. The EG-based real-time PCR assay did not detect canine distemper virus or mink enteritis virus, and the assay could be used to detect Chinese and American AMDV strains, in contrast to a commercial TaqMan kit that could only detect American AMDV strains. The amplification efficiencies of the EG assay were 104.8% for the Chinese strain and 94.4% for the American strain, and the detection limit was 1 copy/µL of AMDV plasmid or 3 pg/µL of viral DNA (Chinese strain). The intra- and inter-assay variation coefficients of melting temperature were all lower than 0.15%, confirming the high reproducibility of the assay. Forty-five clinical blood samples were simultaneously analyzed using the EG real-time PCR, TaqMan kit and conventional PCR, and the detection rates were 91.1%, 0.0% and 86.7%, respectively. Serum samples were also collected from the corresponding blood samples and tested using the counterimmunoelectrophoresis (CIEP) assay, where positive samples accounted for 24.4% of the 45 samples. In conclusion, EG-based real-time PCR is a rapid, sensitive, universal assay that can be effectively utilized as a reliable and specific tool for detection and quantitation of AMDV.

12.
J Nanosci Nanotechnol ; 20(3): 1709-1714, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492334

RESUMO

In this article, nano-NbC particles toughened Si3N4-based ceramics were prepared by injection moulding and their mechanical properties along with toughening mechanism were studied. An increase of nano-NbC content, gradually homogenizes microstructure of the Si3N4-based ceramics along with increase in its density. However, the fracture toughness and flexural strength first increases and then decreases. The Si3N4-based ceramics demonstrate good comprehensive properties at the 15 wt% nano-NbC content and sintering temperature of 1550 °C (where the density is 85.3%, the flexural strength is 845 MPa, and the fracture toughness is 9.3 MPa·m1/2), Backscattered electron imaging shows that nano-NbC particles can be well dispersed in the Si3N4 ceramic matrix by injection moulding and ceramics are toughened by crack deflection and microcracking effects. It was also found that increasing sintering temperature makes the ß-Si3N4 grain distribution more uniform by reducing the porosity.

13.
Chemosphere ; 239: 124748, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31520971

RESUMO

A total of 100 samples were collected from the sediments of a typical copper mining area, south China. Leaching concentrations of selected heavy metals (Ni, Cd, Cu, Pb, Zn, Ba, As, and Hg) were measured to evaluate their distribution patterns and associated health risk. Leaching concentrations of Cu (3.58 ±â€¯1.49 mg/L), Pb (1.50 ±â€¯1.06 mg/L), and Zn (4.04 ±â€¯1.68 mg/L) were significantly higher than the other metals in the samples. By evaluating the spatial heterogeneity, it was found that leaching metal concentrations did not decrease with environmental gradients, mostly caused by diverse distribution in pollution sources. The hazard index and carcinogenic risk indices showed significant risks of human exposure. For public safety, priority governance should be given to the main pollutants (Cd, Cu, Pb, Zn, and As) in sediments. In future studies, the integrated data will be urgently required for local stakeholders to conduct environmental monitoring and remediation scenarios.

15.
Food Chem ; 303: 125385, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31442899

RESUMO

In this study, the mechanism activated by melatonin treatment at 100 µM for maintaining nutraceutical properties in pomegranate fruits during storage at 4 °C for 120 days was investigated. Our results showed that the higher G6PDH and 6PGDH activities in pomegranate fruits treated with melatonin may be responsible for sufficient supply of intracellular NADPH. Also, higher AA and GSH accumulation in pomegranate fruits treated with melatonin may ascribe to higher APX and GR activities coincided with lower AAO activity. In addition, pomegranate fruits treated with melatonin exhibited significantly higher PAL activity resulting in higher phenols and anthocyanins accumulation as well as higher DPPH scavenging capacity. Additionally, higher AOX gene expression in pomegranate fruits treated with melatonin may be beneficial for ROS scavenging molecules accumulation. Therefore, maintaining nutraceutical properties of pomegranate fruits treated with melatonin may ascribe to sufficient intracellular NADPH supply by promoting G6PDH and 6PGDH activities during cold storage.


Assuntos
Antocianinas/análise , Conservação de Alimentos , Melatonina/farmacologia , Punicaceae/efeitos dos fármacos , Suplementos Nutricionais/análise , Frutas/química , Frutas/efeitos dos fármacos , Punicaceae/química
16.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117418, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31362186

RESUMO

A series of luminescent lanthanide compounds with 2, 3, 5, 6-tetrafluoro-1, 4-benzenedicarboxylate (H2BDC-F4) acting as the organic ligand have been synthesized by solvothermal reaction. The terbium compound (C1: Tb-BDC-F4) and europium compound (C7: Eu-BDC-F4) exhibit intense green and red luminescence respectively upon excitation of the ligand at room temperature, which suggests that H2BDC-F4 ligand can sensitize Tb3+ and Eu3+ ions effectively. The emitting color of the mixed lanthanide compounds is tunable from green to red in the mixed lanthanide compounds TbxEu1-x-BDC-F4 (x = 0.3%, 0.5%, 0.7%, 1%, and 2%), which is caused by energy transfer from Tb3+ to Eu3+. The self-reference ratiometric optical thermometry was realized by fluorescence intensity ratio between Tb3+ green emission (∼544 nm) and Eu3+red emission (∼ 619 nm). The maximum relative sensitivity SR,max is 0.76%K-1@50 K. Furthermore, the emitting color of C5: Eu0.01Tb0.99-BDC-F4 could be tuned from green to orange by temperature in the range of 50 to 300 K. It allows the mixed Eu/Tb-BDC-F4 compounds to be potentially used as sensitive luminescent materials to directly imagine the temperature distribution.

17.
Food Chem ; 306: 125611, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31606631

RESUMO

Aromatic secondary metabolites are closely related to quality attributes of postharvest fruit. In the present study, 20% CO2 was applied to strawberry fruit to investigate the regulation of elevated CO2 on aromatic secondary metabolites. The results showed that elevated CO2 delayed accumulations of anthocyanins, eugenol and lignin. Phenylalanine and tyrosine, the precursors of the above secondary metabolites, were 18.90% and 35.61%, respectively, lower in CO2-treated fruit compared with the control on day 6. Furthermore, enzyme activities and transcriptional profiles analysis showed pentose phosphate pathway and glycolysis were activated by elevated CO2 whereas the aromatic amino acids (AAAs) pathway was inhibited. These results indicated that elevated CO2 restricted carbon flux into aromatic secondary metabolism by inhibiting the AAAs pathway, leading to the decrease of phenylalanine and tyrosine, and thus, delayed the accumulation of aromatic secondary metabolites. In addition, the effect of elevated CO2 was eliminated after transferred CO2-treated fruit to air.

18.
Food Chem ; 306: 125628, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31629297

RESUMO

Microencapsulation of Zingiber officinale essential oil (EO) in polysaccharide, chitosan (CH) and sodium carboxymethyl cellulose (CMC) based on the electrostatic interaction between charged polysaccharides at pH 3.0 in dual delivery system. Ratio variations of CH and CMC in microencapsulation were studied at 1:2, 2:1 and 1:1. This study aimed to evaluate the influence of the encapsulating materials combination on freeze-dried EO powders and to present the mechanisms for loading and releasing EO involved in the preparation of CH/CMC microcapsules. The spectroscopy analysis, physical properties, microstructural, encapsulation efficiency and EO release behavior in obtained EO microparticles were evaluated by using the analysis of fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and gas chromatography mass spectrometry (GC-MS), respectively. Afterwards, the above prepared microcapsules were applied on winter jujube fruit (Ziziphus jujuba Mill.) preservation. Results demonstrated that both the microstructure and stability of microencapsulation were improved in delivery system loading with CH and CMC (1:1) with the encapsulation efficiency of 88.50%, compared to other ratios of CH and CMC (1:2 and 2:1). Furthermore, the microencapsulation had a capacity to control and reduce the EO release, therefore the morphological and sensory quality of jujube fruits in EO delivery system during storage was enhanced significantly (P < 0.05), in comparison to control. Results revealed that the microparticles produced with CH and CMC (1:1) was considered to present better characteristics of microstructure, encapsulation efficiency, as well as to maintain higher nutritional quality for jujube fruit. Thus, EO microencapsulation loaded in CH/CMC-based dual delivery system has potential application and developmental value prospects in food industries.

19.
Phys Occup Ther Pediatr ; 40(1): 31-46, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31282292

RESUMO

Aim: To examine the effects of an upper-extremity, community-based, and power-training intervention.Methods: Twelve participants with cerebral palsy (CP) [8 males, 4 females; mean age 14 years 6 months (SD 5 years 4 months), range 7-24] were randomly assigned to a rest-training (RT; n = 6) or training-rest (n = 6) group in this randomized, cross-over design. Training took place in participants' home or school, three times per week for 6 weeks. We examined changes in upper extremity average power output (Pavg) in watts (W) and changes in function via the Pediatric Outcomes Data Collection Instrument (PODCI).Results: Each participant completed at least 15 of the 18 total training sessions (91.2% adherence). Pavg increased 92.2% on average among participants (p < .05). There was a significant three-way interaction among treatment, sequence, and period with the data stratified by (Bimanual Fine Motor Function [BFMF]) level on the pain subscale of the PODCI (p = 0.0118). All participants decreased pain after training with the exception of individuals with lower functioning (BFMF II-V) in the RT group.Conclusion: A community-based upper extremity power-training intervention was feasible and effective at improving power among young people with CP and has the potential to improve pain.

20.
J Hazard Mater ; : 121431, 2019 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-31672436

RESUMO

Spermatogenesis-related microRNAs (miRNAs) are vulnerable to polycyclic aromatic hydrocarbons (PAHs). Changes in spermatogenesis-related miRNAs may be biological intermedia in mechanisms linking PAHs and semen quality. This study aimed to investigate whether spermatogenesis-related microRNAs mediate the associations between PAHs and semen quality. We measured 10 monohydroxylated PAHs (OH-PAHs) in repeated urine samples and three candidate spermatogenesis-related miRNAs (miRNA106a, miRNA21, and miRNA34c) in seminal plasma from men attending an infertility clinic (n = 111). Mediation analysis was applied to determine the mediating role of spermatogenesis-related miRNAs in the association of PAH exposure with semen quality. Urinary 2-OHFlu and 2-OHPh were related to reduced seminal plasma miRNA34c (p for trend = 0.05 and 0.03, respectively). Urinary 9-OHPh was related to reduced seminal plasma miR106a (p for trend = 0.02), which in turn, was positively associated with sperm concentration, sperm count, sperm total motility, and progressive motility (all p for trends<0.05). Up to 43.8% of the eff ;ect of urinary 9-OHPh on decreased sperm concentration was mediated by seminal plasma miR106a. Our results suggested that certain PAH exposure was associated with reduced spermatogenesis-related miRNAs and such alterations might be an intermediate mechanism by which PAHs exert its adverse effects on semen quality.

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