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1.
PhytoKeys ; 184: 19-26, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34759725

RESUMO

Saxifragaviridiflora, a remarkable new species of the genus Saxifragasect.Irregulares (Saxifragaceae) from Guangxi, is described and illustrated herein. This new species morphologically differs from all known S.sect.Irregulares taxa by its greenish petals, verruculose sepals, and thick leathery leaf blade abaxially scarlet with white spots.

2.
Front Oncol ; 11: 692951, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34307157

RESUMO

Acute leukemia (AL) is a highly heterogeneous hematologic malignancy, and although great progress has been made in the treatment of AL with allogeneic hematopoietic stem cell transplantation (Allo-HSCT) and new targeted drugs, problems such as infection and GVHD in AL treatment are still serious. How to reduce the incidence of AL, improve its prognosis and reduce the side effects of treatment is a crucial issue. The gut microbiota plays an important role in regulating disease progression, pathogen colonization, and immune responses. This article reviews recent advances in the gut microbiota and AL pathogenesis, infection, treatment and its role in allo-HSCT.

3.
Acta Otolaryngol ; 141(8): 802-807, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34314296

RESUMO

BACKGROUND: The diagnosis of vocal cord leukoplakia mainly relies on laryngoscopy. The morphology of vocal cord leukoplakia under laryngoscope is closely related to the pathological nature of leukoplakia. The specific manifestations associated with high-risk vocal cord leukoplakia remain to be explored. OBJECTIVE: To investigate the characteristics of low-risk and high-risk vocal cord leukoplakia under conventional white light imaging (WLI) laryngoscopy and its correlations with narrow band imaging (NBI) laryngoscopy. METHODS: One hundred and seventy-five cases of vocal cord leukoplakia were divided into low-risk and high-risk groups. The characteristics of low-risk and high-risk vocal cord leukoplakia under WLI laryngoscopy and its correlations with NBI laryngoscopy were analyzed. RESULTS: Logistic regression analysis showed that thickness and hyperemia were independent factors (p < .05). Hyperemia had a strong consistency with the visualization of spots under NBI laryngoscopy (kappa = 0.758). The sign of hyperemia and the NBI classification had equivalent diagnostic efficacy for predicting the risk of cancerization of vocal cord leukoplakia. CONCLUSION: The sign of hyperemia under WLI laryngoscopy is significantly correlated with the visualized spots under NBI laryngoscopy. Hyperemia is an important feature for predicting malignant potential of vocal cord leukoplakia.

4.
Exp Ther Med ; 22(2): 873, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34194551

RESUMO

MicroRNAs (miRNAs/miRs) serve an important role in the pathogenesis of chronic heart failure (CHF). A number of reports have illustrated the regulatory effect of serum exosomal miRNA on myocardial fibrosis. The present study aimed to investigate the expression of miR-320a in serum exosomes, as well as the effect of miR-320a on myocardial fibroblast proliferation. Serum exosome samples from 10 patients with CHF and 5 healthy volunteers were obtained and characterized. mRNA and protein expression levels were measured via reverse transcription-quantitative PCR and western blotting, respectively. The content of soluble growth stimulation expressed gene 2 (sST2) was determined via ELISA. HEH2 cell viability and apoptosis were detected by performing MTT assays and flow cytometry, respectively. The results demonstrated that serum miR-320a expression levels and sST2 content were significantly increased in patients with CHF compared with healthy controls, and the expression of serum miR-320a was significantly correlated with clinical CHF indexes. miR-320a expression levels were significantly increased in exosomes isolated from patients with CHF compared with those isolated from healthy controls. Phosphoinositide-3-kinase catalytic α polypeptide gene (PIK3CA) expression levels and sST2 content were increased in HEH2 cells following transfection with miR-320a mimics compared with NC-mimic, whereas miR-320a inhibitor displayed contrasting effects by reduced the cell viability and apoptosis in myocardial fibroblasts compared with the NC-inhibitor group. The protein expression levels of collagen I, collagen III, α-smooth muscle actin, phosphorylated (p)-mTOR (ser 2448)/mTOR, p-Akt (ser 473)/Akt, p-Akt (thr 308)/Akt and PIK3CA were significantly increased in miR-320a mimic-transfected HEH2 cells compared with the NC-mimics groups. By contrast, miR-320a inhibitor notably downregulated the expression levels of these proteins compared with the NC-inhibitor group. Collectively, the results of the present study demonstrated that miR-320a promoted myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cells, suggesting that serum exosomal miR-320a may serve as a potential biomarker for the diagnosis of CHF.

5.
PhytoKeys ; 178: 179-191, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34163300

RESUMO

Smelowskia sunhangii, from Qinghai and Tibet (China), is described and illustrated. Morphological and molecular data indicate that S. sunhangii is closely related to Smelowskia tibetica, from which it is easily distinguished by the densely hirsute (vs. glabrous or sparsely pubescent), elliptic to ovate-lanceolate (vs. suborbicular, oblong, or lanceolate) fruits with undulate (vs. straight) margins. A re-evaluation of the widely distributed S. tibetica and related taxa is also provided.

6.
Mitochondrial DNA B Resour ; 6(6): 1784-1785, 2021 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-34104774

RESUMO

Centranthera grandiflora is an important medicinal herb within Orobanchaceae. To date, however, genetic studies on this species remain poor. Here, we assembled the complete chloroplast genome of C. grandiflora. Results showed that the genome was 147 655 bp in length, consisting of large and small single copy regions of length 83 550 and 14 891 bp, respectively, separated by two inverted repeat regions of 24 607 bp. Furthermore, the genome contained 132 genes, including 84 protein-coding genes, 39 tRNA genes, and eight rRNA genes. Phylogenetic analysis showed that C. grandiflora is closely related to the species of Orobanchaceae. The complete chloroplast genome of C. grandiflora should help in the conservation of genetic resources and appropriate utilization of this medicinal herb in the future.

7.
Interact Cardiovasc Thorac Surg ; 33(2): 301-308, 2021 07 26.
Artigo em Inglês | MEDLINE | ID: mdl-33822951

RESUMO

OBJECTIVES: The postoperative risk factors for electroencephalogram(EEG) abnormalities after paediatric cardiopulmonary bypass (CPB) remain to be identified. We investigated the characteristics of EEG abnormalities and risk factors in routine clinical management post-CPB. METHODS: EEG and cerebral oxygen saturation (ScO2) were monitored in 96 patients (aged 3 days, 37 months, median 5 months) for 72 h post-CPB. Clinical measurements included 4-hourly arterial and central venous pressure, arterial blood gases, doses of inotropic and vasoactive drugs, daily C-reactive protein (CRP) and NT-proB-type Natriuretic Peptide (NT-proBNP). Demographics, STAT categories and outcomes (duration of mechanical ventilation,CICU stay) were recorded. Un. RESULTS: Seizures occurred in 20 patients (20.8%) beginning at 0-48 hand lasting 10 min-31 h; background abnormalities occurred in 67 (69.8%) beginning at 0-8 h and lasting 4-48 h. Patients with EEG abnormalities had worse outcomes. In univariable regression, seizures positively correlated with STAT categories, CPB time, temperature, blood pressure, central venous pressure, NT-proBNP, CRP, lactate and epinephrine, negatively with ScO2 and PaCO2 (P < 0.001 for lactate and epinephrine, P < 0.1 for the remaining). The degree of background abnormalities positively correlated with STAT categories, CPB time, operative time, central venous pressure, milrinone, negatively with blood pressure (P = 0.0003-0.087); it negatively correlated with lower dose of epinephrine (P < 0.001) and positively with higher dose (P = 0.03l). In multivariable regression, seizures positively correlated with epinephrine, lactate and temperature; the background abnormality correlations remain significant except for milrinone and operative time (P < 0.001 for epinephrine, P < 0.05 for the remaining). CONCLUSIONS: Numerous perioperative risk factors are associated with EEG abnormalities post-CPB. The most significant and consistent risk factor is epinephrine.


Assuntos
Ponte Cardiopulmonar , Oxigênio , Ponte Cardiopulmonar/efeitos adversos , Criança , Eletroencefalografia , Humanos , Período Pós-Operatório , Fatores de Risco
8.
Ann Transl Med ; 9(2): 153, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33569455

RESUMO

Background: Anti-soluble liver antigen/liver pancreas (anti-SLA/LP) is a highly specific serological marker for the diagnosis of autoimmune hepatitis (AIH). The aim of the present study was to define the clinical characteristics and human leucocyte antigen (HLA) genotypes of Chinese patients with anti-SLA/LP positive AIH. Methods: Ninety-one AIH patients who were anti-SLA/LP positive were enrolled in this case control study. Clinical information was obtained through reviewing patients' clinical notes. High-resolution genotyping of HLA-A, B, C, DRB1, and DQB1 alleles was performed by sequence-based typing polymerase chain reaction on 62 of the 91 patients. Data from 500 healthy patients were used as baseline controls. Results: Anti-SLA/LP-positive AIH patients were characterized as follows: adults (age 20-80 years), female (88%), and frequent anti-nuclear antibody positivity (91%). Genetically, compared with the controls, HLA-B*35:01 and C*08:01 were significantly more frequent in patients. The frequencies of HLA-B*08:01, B*40:02, DRB1*04:01, DRB1*04:05, DRB1*14:01, and DRB1*16:02 increased, and the frequency in DRB1*15:01 decreased in patients, but did not reach significance after Bonferroni's correction. Patients with other autoimmune diseases had a higher DRB1*04:05 and DQB1*04:01 allele carrier frequency than those without. DRB1*04:05 and DQB1*04:01 alleles were found at increased frequency in patients with decompensated liver disease than those with compensated liver disease. Conclusions: Chinese anti-SLA/LP-positive AIH patients have some distinct clinical characteristics than other populations reported in the literature. The presence of certain specific HLA alleles could potentially increase the risk of developing anti-SLA/LP-positive AIH or other autoimmune disease and decompensated liver disease in the Chinese population.

9.
Environ Sci Pollut Res Int ; 28(14): 18050-18061, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33410055

RESUMO

Arsenic (As)-contaminated paddy soil could result in elevated levels of As in rice plants and sequentially harm human health. The Fe3O4-modified biochar (NBC-Fe) prepared by the coprecipitation method was applied in a pot experiment to investigate its effect on mobility and bioavailability of As in soil and to reduce As accumulation in rice tissues (brown rice, husks, spikelets, leaves, stems, and roots). Compared with non-application (CK), application of NBC-Fe significantly increased the cation exchange capacity (CEC), decreased As availability, and raised the As concentration of crystalline hydrous oxide-bound fraction in the soil. The addition of 0.05-1.6% (w/w) NBC-Fe significantly reduced the As concentrations in brown rice by 9.4-47.3%, which was lower than the level set by the National Food Safety Standards of China (0.2 mg/kg). The NBC-Fe treatment decreased As concentrations in iron plaque (DCB-As), and the DCB-As had the very significant correlations (P < 0.01) with the As concentrations in different rice tissues (brown rice, husks, spikelets, leaves, stems, and roots). The NBC-Fe immobilized As to decrease As availability in soil and increased the amount and thickness of iron plaque to sequester As on the surfaces of rice root. This study demonstrates that NBC-Fe is a promising soil amendment for the remediation of As-contaminated soil, therefore reducing As accumulation in rice plant and safety risks for rice consumption.


Assuntos
Arsênio , Oryza , Poluentes do Solo , Arsênio/análise , Cádmio/análise , Carvão Vegetal , China , Humanos , Raízes de Plantas/química , Solo , Poluentes do Solo/análise
10.
Mitochondrial DNA B Resour ; 5(1): 366-367, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33366559

RESUMO

A complete chloroplast genome of Actinidia rubus, an endemic shrub in China, was sequenced and identified. The length of genome is 156,573 bp, and the GC content is 37.3%. This genome contains a large single copy (LSC; 88,473 bp) region, a small single copy (SSC; 20,492) region, a pair of inverted repeat (IR; 23,804) regions. A total of 113 unique genes were identified, including 78 protein-coding genes, 31 tRNA genes and 4 rRNA genes. The phylogenetic analysis based on complete chloroplast genome of 10 species showed that Actintdia eriantha was sister to A. rubus.

11.
Aging (Albany NY) ; 12(24): 25256-25274, 2020 11 20.
Artigo em Inglês | MEDLINE | ID: mdl-33226370

RESUMO

In this meta-analysis, we systematically investigated the correlation between single nucleotide polymorphisms (SNPs) and pancreatic cancer (PC) risk. We searched PubMed, Network Science, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), China Science and Technology Periodical Database (VIP), and Wanfang databases up to January 2020 for studies on PC risk-associated SNPs. We identified 45 case-control studies (36,360 PC patients and 54,752 non-cancer individuals) relating to investigations of 27 genes and 54 SNPs for this meta-analysis. Direct meta-analysis followed by network meta-analysis and Thakkinstian algorithm analysis showed that homozygous genetic models for CTLA-4 rs231775 (OR =0.326; 95% CI: 0.218-0.488) and VDR rs2228570 (OR = 1.976; 95% CI: 1.496-2.611) and additive gene model for TP53 rs9895829 (OR = 1.231; 95% CI: 1.143-1.326) were significantly associated with PC risk. TP53 rs9895829 was the most optimal SNP for diagnosing PC susceptibility with a false positive report probability < 0.2 at a stringent prior probability value of 0.00001. This systematic review and meta-analysis suggest that TP53 rs9895829, VDR rs2228570, and CTLA-4 rs231775 are significantly associated with PC risk. We also demonstrate that TP53 rs9895829 is a potential diagnostic biomarker for estimating PC risk.


Assuntos
Antígeno CTLA-4/genética , Predisposição Genética para Doença/genética , Neoplasias Pancreáticas/genética , Receptores de Calcitriol/genética , Proteína Supressora de Tumor p53/genética , Humanos , Metanálise em Rede , Polimorfismo de Nucleotídeo Único/genética
12.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(5): 1661-1667, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33067970

RESUMO

OBJECTIVE: To investigate the expression of IL-9 and IL-6 in patients with BCR-ABL- bone marrow proli- ferative tumor (MPN), and to explore its role in the occurrence and development of MPN. METHODS: A total of 71 newly diagnosis MPN patients treated in Tianjin Medical University General Hospital from 2018 to 2019 were selected, including 32 patients with polycythemia vera (PV) and 22 patients with primary thrombocytosis (ET), and 17 patients with primary myelofibrosis (PMF). Then 58 patients who retestine after treatment were selected as therapy group,and 20 healthy volunteers were recruited as control group. ELISA was used to detect the expression level of IL-6 and IL-9 in bone marrow supernatant, and the relative expression level of IL-6 and IL-9 mRNA in BMMNC was detected by real-time PCR. The proportion of Th9 cells in peripheral blood were detected by flow cytometry (FCM). The expression level of IL-6 mRNA and IL-9 mRNA of BMMNC and clinical indicators were analyzed, and the correlation between JAK2 gene mutation load and IL-9 level was further analyzed. RESULT: The level of IL-6 in bone marrow supernatant and the expression of IL-6 mRNA in BMMNC were higher in the newly diagnosed group as compared with those in the treated group and the control group (P<0.001). The expression level of IL-9 in bone marrow supernatant and the expression of IL-9 mRNA in BMMNC were lower in the newly diagnosed group as compared with those in the treated group and the control group (P<0.05). The proportion of Th9 cells in peripheral blood was lower in the newly diagnosed group as compared with that in the treated group and the control group (P<0.001). The level of IL-6 in bone marrow supernatant and the expression of IL-6 mRNA in BMMNC in JAK2+ group were higher than those in JAK2- group (P<0.05). The expression level of IL-9 in bone marrow supernatant and the expression of IL-9 mRNA in BMMNC were lower in JAK2+ group as compared with those in JAK2- group (P<0.05). The expression of IL-6 and IL-9 in the patient group showed correlation with the number of lymphocytes (IL-6: r=-0.49, P<0.01; IL-9: r=0.53, P<0.001), and also related with Hb in PV patients (IL-6: r= 0.87, P<0.001; IL-9: r=-0.54, P<0.01), and platelets in ET patients (IL-6: r=0.64, P<0.05; IL-9: r=-0.46, P<0.05). CONCLUSION: The increased expression of IL-6 in MPN and hyperfunction may promote the progression of BCR-ABL- MPN disease. The expression of IL-9 in MPN decreases, and it negatively correlates with the mutation load of JAK2 gene, which may be related with the decrease of tumor environmental antitumor immune effect.


Assuntos
Transtornos Mieloproliferativos , Trombocitemia Essencial , Proteínas de Fusão bcr-abl/genética , Humanos , Interleucina-6 , Interleucina-9
13.
Cancer Manag Res ; 12: 8935-8941, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33061589

RESUMO

Objective: To assess the short-term efficacy and safety of ixazomib in Chinese multiple myeloma (MM) patients in the real world. Methods: Fifty-nine MM patients who received at least one cycle of ixazomib-based therapy between 1 June 2018 and 30 September 2019 were retrospectively analyzed in Tianjin Medical University General Hospital. Thirteen newly diagnosed MM (NDMM), 13 refractory/relapsed MM (RRMM) and 33 continuous therapy (27 bortezomib peripheral neuritis (PN) intolerant and six maintenance therapy) MM patients were included. The indicated overall response rate (ORR), time to overall response (TOR), and adverse events (AEs) were investigated. Results: The ORR in NDMM was 76.9%, with one complete response (CR), five very good partial response (VGPR), four partial response (PR), median PFS, and TOR were 122 (66-272) days and 49 (22-108) days. The ORR in RRMM was 46.2%, with one CR, two VGPR, three PR, median PFS, and TOR were 79 (28-169) days and 59 (23-88) days. The ORR in continuous therapy MM patients was 100%, with nine stringent CR, 15 CR, six VGPR and three PR, median TOR was 75 (25-141) days. There were no significant differences regarding ORR between patients with cytogenetic high risk and standard risk in three subgroups (all P>0.05). The most frequent hematological AEs were anemia (13.6%) and thrombocytopenia (10.2%). The most common nonhematological AEs were PN (25.0%) and diarrhea (13.6%). Conclusion: The real-world data demonstrated that ixazomib-based therapy was generally effective and safe in the short term for MM patients.

14.
Medicine (Baltimore) ; 99(31): e21310, 2020 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-32756114

RESUMO

BACKGROUND: Since December 2019, there have been many cases of viral pneumonia of unknown causes in Wuhan City, Hubei Province. During the period of novel coronavirus, according to the observation of limited autopsy and biopsy pathological results, pulmonary interstitial fibrosis appeared in some pathological changes of lung. Idiopathic pulmonary fibrosis (IPF) is a chronic progressive interstitial pneumonia with unknown etiology and pathological changes limited to the lung. At present, there is still a lack of reevaluation of systematic evaluation of traditional Chinese medicine treatment IPF. Therefore, a systematic re-evaluation of the systematic evaluation of traditional Chinese medicine in the treatment of pulmonary fibrosis may help to understand the effective treatment scheme of traditional Chinese medicine in the treatment of pulmonary fibrosis and provide more reliable evidence for the first-line clinicians to treat novel coronavirus. METHODS: We will search 3 foreign electronic databases (Cochrane Library, Embase, PubMed) and 4 Chinese electronic databases (China National Knowledge Infrastructure [CNKI], WangFang Database, Chinese Biomedical Literature Database [CBM], and Chinese Scientific Journal Database [VIP]) to collect potential systematic reviews from their inceptions to February 2020. The language of publication is limited to Chinese or English. We will consider SRs and meta-analysis of Traditional Chinese Medicine for the Treatment of pulmonary fibrosis. Two reviewers will identify relevant studies, and then assess the methodological quality by assessment of multiple systematic reviews-2 tool. Using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) report checklist to assess the quality of reports included in the study. In order to better evaluate the systematic evaluation included in this research, risk of bias in systematic review tool is included in this research to evaluate the methodological quality. The quality of evidence of the included systematic reviews was assessed by the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. The Primary outcomes include: Clinical total effective rate, curative effect of TCM symptoms, pulmonary function and blood gas analysis. RESULTS: The results of this study will be published in a peer-reviewed journal. CONCLUSIONS: We expect to obtain reliable evidence from systematic analysis of traditional Chinese medicine treatment of pulmonary fibrosis in an available and useful document. REGISTRATION NUMBER: INPLASY202060029.


Assuntos
Betacoronavirus , Infecções por Coronavirus/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Medicina Tradicional Chinesa/métodos , Pneumonia Viral/tratamento farmacológico , Fibrose Pulmonar/tratamento farmacológico , COVID-19 , Infecções por Coronavirus/complicações , Feminino , Humanos , Masculino , Metanálise como Assunto , Pandemias , Pneumonia Viral/complicações , Fibrose Pulmonar/virologia , Projetos de Pesquisa , SARS-CoV-2 , Revisões Sistemáticas como Assunto , Resultado do Tratamento
15.
Huan Jing Ke Xue ; 41(6): 2898-2907, 2020 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608807

RESUMO

Plants modify the soil microenvironment through root exudation. It is important to study the dynamic changes of soil ecosystem from the perspective of root-soil-microbe interactions after vegetation restoration in the riparian zone of the Three Gorges Reservoir (TGR). The rhizosphere and bulk soils of Cynodon dactylon, Hemarthria altissima, Taxodium distichum, and Salix matsudana were collected from the vegetation restoration demonstration base of Ruxi River to explore the differences in nutrient contents and enzyme activities between the rhizosphere and bulk soils. At the same time, the diversity of the bacterial community in the rhizosphere and bulk soils was also investigated using the high throughput sequencing method, with the aim to clarify the growth adaptabilities and nutritional utilization strategies within a more precise rhizosphere range. The results showed that ① Suitable plants enhanced the transformation efficiency of rhizosphere nutrients in different ways to improve their adaptability to the soil environment in the TGR. Compared with bulk soil, root activities had significant effects on nutrient contents in the rhizosphere. Among them, SOC, AN, TN, and AP were enriched significantly to a certain degree, while the changes of potassium were not consistent in different plant species. ② In the process of vegetation restoration, the deposition of litter and root secretion indirectly regulated soil enzyme activity. Invertase, urease, and acid phosphatase, all exhibited positive rhizosphere effects (R/S>1) in these four suitable plant species. However, considering the differences in root structure and physiological characteristics between herbaceous and woody plants, the rhizosphere effect of these three enzymes in four plants was different. ③ The results of high-throughput sequencing showed that there was no significant difference in bacterial community diversity between the rhizosphere and bulk soil of four suitable plant species in the TGR. In addition, Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, Bacteroidetes, Planctomycetes, Cyanobacteria, Firmicutes, Nitrospirae, Gemmatimonadetes, WS3, and Crenarchaeota were the twelve most abundant bacterial phyla in the rhizosphere and bulk soils, serving the ecological functions of nutrition absorption and disease suppression. Their colonization was found to be beneficial to the stress resistance of plants growing in harsh riparian ecosystems in the TGR.


Assuntos
Ecossistema , Solo , Nutrientes , Raízes de Plantas , Plantas , Rizosfera , Microbiologia do Solo
16.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(4): 480-487, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32691554

RESUMO

Objective: To investigate the effects and the mechanism of geniposide on the neuroinflammation occured in the neurodegeneration course of a chronic cerebral hypoperfusion rat model. Methods: Permanent bilateral common carotid arteries occlusions was performed to induce gradient cognitive deficit in rats. The sham group was used as control group. Then 18 rats that met the Screening Criteria were randomly selected 8 weeks post surgery, and were randomly divided into three groups, the 2-VO rats with saline solution group (2-VO+saline group), 2-VO rats with 50 mg/kg per day geniposide group (2-VO+G50) and 2-VO rats with 100 mg/kg per day geniposide group (2-VO+G100). All intervention groups were daily administered with geniposide or saline for 4 weeks. The sham-operated rats were administrated with saline. Then the rats were tested for Morris water maze to evaluate the memory and learning ability. Rats were sacrificed to obtain cortex and hippocampus tissues for HE staining and to detect expression level of glial fibrillary acidic protein (GFAP), inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB), and the level of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin (IL)-6. Results: The 2-VO+saline group rats showed significant longer escape latency and less percent time in target quadrant, compared with sham-operation group ( P<0.05). The escape latency of 2-VO+G50 and 2-VO+G100 groups were shorter than the 2-VO+saline group ( P<0.05), but still longer than the sham group ( P<0.05), the percent time in target quadrant of which were more than the 2-VO+saline group and less than the sham group. However, there was no significant difference between these two groups. HE staining of sham group showed that neurons in the cortex and hippocampus lined up in order, cellar nucleus were big and globular. HE staining results showed that there were obviously neuoral cells loss, severe cytomorphosis, structural disappearance and nuclear fragmentation in the 2-VO+saline group. The 2-VO+G50 and 2-VO+G100 groups showed less neurodamage than the 2-VO+saline group with less neuoral cells loss, cytomorphosis and ambiguous nucleus. GFAP, iNOS, NF-κB were all highly expressed in the process of cognitive dysfunction in rats after chronic cerebral ischemia, however geniposide intervention (50 and 100 mg/kg per day) significantly decreased the expression of the above proteins. In addition, much more TNF-α and IL-6 were released in brain induced by chronic cerebral ischemia, and the levels were decreased after chronic geniposide oral treatment. No significant differences were detected between 2-VO+G50 and 2-VO+G100 groups. Conclusion: These findings demonstrated that geniposide significantly prevented cognition deterioration induced by chronic cerebral hypoperfusion in rats. Geniposide inhibited neuroinflammation occurred in the process of chronic cerebral ischemia probably via reducing iNOS and NF-κB expression and suppressing the release of inflammatory factor TNF-α and IL-6.


Assuntos
Isquemia Encefálica , Transtornos Cognitivos , Hipocampo , Iridoides , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Isquemia Encefálica/complicações , Isquemia Encefálica/tratamento farmacológico , Modelos Animais de Doenças , Hipocampo/efeitos dos fármacos , Iridoides/farmacologia , Iridoides/uso terapêutico , Aprendizagem em Labirinto/efeitos dos fármacos , Distribuição Aleatória , Ratos
17.
Opt Express ; 28(14): 19901-19915, 2020 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-32680060

RESUMO

We propose a THz nondestructive analysis method based on multiple echoes and feature fusion. Conventionally, it is difficult to identify the debonding defects of the glue layer (II) due to the thin adhesive layer. To this end, a THz propagation model is established, and a quantitative method for determining the thickness of debonding defects based on multiple echoes is presented. The measurement error for preset defect thickness of 500 µm was 4%. Further, for determining the area of debonding defects, a feature fusion imaging algorithm is proposed to realize the lateral recognition of defects and quantitative analysis is used to improve the recognition ability of defects.

18.
Medicine (Baltimore) ; 99(24): e20345, 2020 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-32541456

RESUMO

BACKGROUND: Single nucleotide polymorphisms (SNPs) have been inconsistently associated with pancreatic cancer (PC) risk. This meta-analysis aimed to synthesize relevant data on SNPs associated with PC. METHODS: Databases were searched to identify association studies of SNPs and PC published through January 2020 from the databases of PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Infrastructure, the Chinese Science and Technology Periodical Database (VIP) and Wanfang databases. Network meta-analysis and Thakkinstian algorithm were used to select the most appropriate genetic model, along with false positive report probability (FPRP) for noteworthy associations. The methodological quality of data was assessed based on the STREGA statement Stata 14.0 will be used for systematic review and meta-analysis. RESULTS: This study will provide a high-quality evidence to find the SNP most associated with pancreatic cancer susceptibility and the best genetic model. CONCLUSIONS: This study will explore which SNP is most associated with pancreatic cancer susceptibility.Registration: INPLASY202040023.


Assuntos
Neoplasias Pancreáticas/epidemiologia , Neoplasias Pancreáticas/genética , Polimorfismo de Nucleotídeo Único/genética , Algoritmos , Estudos de Casos e Controles , China/epidemiologia , Reações Falso-Positivas , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Metanálise em Rede , Neoplasias Pancreáticas/sangue , Neoplasias Pancreáticas/mortalidade , Risco , Sensibilidade e Especificidade
19.
MycoKeys ; 66: 135-157, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32377155

RESUMO

New collections of six Squamarina species from type localities in China were studied. The comparison of morphological characteristics and secondary metabolites with those of the type specimens and phylogenetic analyses suggest that S. callichroa and S. pachyphylla belong to Rhizoplaca, S. semisterilis belongs to Lobothallia and S. chondroderma should be retained in Lecanora temporarily. Only two species, S. kansuensis and S. oleosa, remain in Squamarina. The new combinations Lobothallia semisterilis (H. Magn.) Y. Y. Zhang, Rhizoplaca callichroa (Zahlbr.) Y. Y. Zhang and R. pachyphylla (H. Magn.) Y. Y. Zhang are proposed. Detailed descriptions to aid the identification of these species, distributions and phylogenetic trees, based on multiple collections, are presented. The generic concept of Squamarina is recircumscribed in this study.

20.
Vet Microbiol ; 240: 108542, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31902499

RESUMO

Influenza A virus (IAV) and bacteria co-infection can influence the host clinical conditions. Both H9N2 IAV and Pseudomonas aeruginosa (P. aeruginosa) are potential pathogens of respiratory diseases in mink. In this study, to clarify the effects of H9N2 IAV and P. aeruginosa co-infections on hemorrhagic pneumonia in mink, we carried out to establish the mink models of the two-pathogen co-infections in different orders. Compared with the single infections with H9N2 IAV or P. aeruginosa, the mink co-infected with H9N2 IAV and P. aeruginosa showed severe respiratory diseases, and exacerbated histopathological lesions and more obvious apoptosis in the lung tissues. H9N2 IAV shedding and viral loads in the lungs of the mink co-infected with H9N2 IAV and P. aeruginosa were higher than those in the mink with single H9N2 IAV infection. Furthermore, the clearance of P. aeruginosa in the co-infected mink lungs was delayed. In addition, the anti-H9N2 antibody titers in mink with P. aeruginosa co-infection following H9N2 IAV infection were significantly higher than those of the other groups. This implied that H9N2 IAV and P. aeruginosa co-infection contributed to the development of hemorrhagic pneumonia in mink, and that P. aeruginosa should play a major role in the disease. The exact interaction mechanism among H9N2 IAV, P. aeruginosa and the host needs to be further investigated.


Assuntos
Coinfecção/veterinária , Hemorragia/veterinária , Vírus da Influenza A Subtipo H9N2/patogenicidade , Infecções por Orthomyxoviridae/veterinária , Pneumonia/veterinária , Infecções por Pseudomonas/veterinária , Pseudomonas aeruginosa/patogenicidade , Animais , Anticorpos Antivirais/sangue , Coinfecção/microbiologia , Coinfecção/virologia , Hemorragia/microbiologia , Hemorragia/virologia , Humanos , Pulmão/patologia , Pulmão/virologia , Vison/microbiologia , Vison/virologia , Infecções por Orthomyxoviridae/microbiologia , Pneumonia/microbiologia , Pneumonia/virologia , Infecções por Pseudomonas/virologia , Replicação Viral , Eliminação de Partículas Virais
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