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1.
Reprod Sci ; 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-32046461

RESUMO

In response to cytosolic DNA, stimulator of interferon gene (STING) initiates and orchestrates host's innate immunity by inducing type I interferon. Since endometriosis is a chronic inflammatory disorder, we sought to determine whether STING pathway is activated in ectopic endometrium in comparison to eutopic endometrium. Immunohistochemistry was employed in evaluating the expression levels of STING in normal endometrium, endometriosis, and adenomyosis. The density of CD45+ intraepithelial lymphocytes was correlated with STING expression levels. A total of 39 cases of endometriosis and/or adenomyosis with normal endometrium were analyzed. Among them, 32 had adenomyosis, 26 had endometriosis, and 19 have both lesions. STING protein expression is mainly evident in the cytoplasm of epithelial cells but much less in stromal cells. Based on H-score, we found that the STING expression levels were significantly higher in the epithelial cells of adenomyosis and endometriosis than in eutopic endometrium (132.7 ± 12.20, 119.6 ± 12.57 vs. 19.74 ± 5.96, p < 0.0001). There was no significant difference in STING expression level between endometriosis and adenomyosis. More intraepithelial lymphocytes were detected in endometriosis and adenomyosis lesions than endometrium (5.60 ± 0.70%, 4.95 ± 0.54% vs. 1.25 ± 0.12%, p < 0.0001). A positive correlation between STING expression and intraepithelial lymphocytic infiltrate was observed (p < 0.0001). In summary, STING was upregulated in the epithelium of ectopic endometrium as compared to eutopic endometrium. Its expression levels correlate with the degree of intraepithelial lymphocyte infiltration, suggesting a role in promoting chronic inflammation of ectopic endometrium.

2.
Clin Exp Pharmacol Physiol ; 47(1): 135-142, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31557334

RESUMO

An increasing number of studies have shown that kinesin family member 20A (KIF20A) was overexpressed in several types of cancer, and its overexpression correlated with the oncogenesis and prognosis of cancers. However, little is known about the roles of KIF20A in human non-small cell lung cancer (NSCLC). Thus, the aim of the present study was to demonstrate the expression of KIF20A in human NSCLC and reveal its biological functions and the underlying mechanisms. qRT-PCR, western blot and immunohistochemistry were used to assess the expression of NSCLC patient specimens and NSCLC cell lines. The functions of KIF20A in migration and invasion were determined using Transwell assay. Cell proliferation capacity was performed by CKK-8 assay. We demonstrated that KIF20A was overexpressed in NSCLC specimens compared with the adjacent non-tumorous specimens, and high expression of KIF20A was associated with clinical stage and metastasis in NSCLC. Decreased expression of KIF20A inhibited NSCLC cells migration, invasion and proliferation. Most importantly, further experiments demonstrated that decreased the expression of KLF20A significantly downregulated expression of p-JNK and MMP7, which indicated that knockdown of KIF20A alters lung cancer cell phenotype and regulates JNK pathways. These results suggest that KIF20A may act as a putative oncogene and a potential therapeutic target in NSCLC.

3.
Zhongguo Fei Ai Za Zhi ; 22(12): 747-751, 2019 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-31874668

RESUMO

Venous thromboembolism (VTE) is a common perioperative complication in patients with thoracic malignant tumor. Once it occurs, it will not only affect the prognosis of patients, but also occupy a lot of medical resources, which is gradually causing our widespread attention. However, the understanding of VTE in thoracic surgery in our country is relatively late, and the recognition and attention are not enough, and there is still a lack of guidance support for perioperative VTE. Based on the current understanding and preventive measures of VTE in thoracic surgery in China, The China National Research Collaborative Group released the first edition of Chinese experts consensus on the perioperative VTE prophylaxis in 2018. This article will interpret the high-risk patients with perioperative VTE in patients with thoracic malignant tumors, in order to provide a better understanding of Chinese experts consensus for readers.

4.
Sci Rep ; 9(1): 15616, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666604

RESUMO

MicroRNAs are known to play essential role in the gene expression regulation in cancer. In our research, next-generation sequencing technology was applied to explore the abnormal miRNA expression of oral squamous cell carcinoma (OSCC) in Chinese hamster. A total of 3 novel miRNAs (Novel-117, Novel-118, and Novel-135) and 11 known miRNAs (crg-miR-130b-3p, crg-miR-142-5p, crg-miR-21-3p, crg-miR-21-5p, crg-miR-542-3p, crg-miR-486-3p, crg-miR-499-5p, crg-miR-504, crg-miR-34c-5p, crg-miR-34b-5p and crg-miR-34c-3p) were identified. We conducted functional analysis, finding that 340 biological processes, 47 cell components, 46 molecular functions were associated with OSCC. Meanwhile the gene expression of Caspase-9, Caspase-3, Bax, and Bcl-2 were determined by qRT-PCR and the protein expression of PTEN and p-AKT by immunohistochemistry. Our research proposed further insights to the profiles of these miRNAs and provided a basis for investigating the regulatory mechanisms involved in oral cancer research.

5.
Med Phys ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31742714

RESUMO

PURPOSE: As a novel imaging modality, x-ray fluorescence computed tomography (XFCT) can provide distribution and concentration information of contrast agents containing high atomic number elements, such as iodine, gadolinium, barium, gold, and platinum. Since XFCT has a better sensitivity and detection limit of high-Z elements compared with traditional and spectral CT, it becomes a powerful quantitative imaging tool for biological studies. The main problem of current XFCT imaging is its low emission and detection efficiency of x-ray fluorescence (XRF) photons. Increasing XRF photons generation by choosing a high atomic element as a contrast agent is essential to improve the imaging quality of XFCT. Gadolinium emits at least a few times more of XRF photons than gold under the same x-ray excitation condition, leading to a detection limit at a level of sub-mg/mL as the next generation of clinical imaging modality. However, most current XFCT studies have utilized gadolinium salt as the contrast agent, which could not accumulate in organs or tumors efficiently, making in vivo XFCT imaging quite difficult. In this study, we present NaGdF4 nanoparticles with ultra-small size as nanoprobes to test the feasibility for in vivo XFCT application for the first time. METHODS: NaGdF4 nanoparticles with different sizes (3-10 nm) were successfully synthesized via a coprecipitation process by controlling the reaction time at temperature of 290 °C. The morphology, crystal phase, chemical composition, and size of such NPs were further characterized with HR-TEM, XRD, and EDX. The abilities of XRF photons from different sizes of NPs were quantified by our customized XFCT imaging system. To access the in vivo application of as-synthesized NPs, such hydrophobic NPs capped OA molecules were further modified with AEP via a ligand-exchange process and characterized with FT-IR. For in vivo XFCT imaging, 0.1 mL of 30 mg/mL NPs were injected into nude mice via the tail vein. The Varian G-297 x-ray tube was set to 150 kV and 0.5 mA. The XRF photons were captured by a Kromek eV-3500 photon counting detector at each 8° for 10 s. RESULT: The successfully synthesized NaGdF4 nanoparticles (3-10 nm) were monodisperse, highly uniform spherical morphology and hexagonal crystalline phases. No significant influence on XRF photons yields or XFCT imaging quality were found by varying the nanoparticle size. The XRF photons were 2.5 times more emitted from NaGdF4 nanoparticles (NPs) compared to gold nanoparticles, thereby leading to a better image quality. With the AEP surface modification, such NPs were readily adapted for use in in vivo XFCT applications with monodispersity in aqueous solution and negligible cytotoxicity. With the tail-vein injection, the liver, spleen, and lungs could be clearly imaged with XFCT at a sub-mg/mL level. CONCLUSION: Such NaGdF4 NPs, which were synthesized with coprecipitation process, were modified with AEP for in vivo XFCT applications. With both the phantom and in vivo experiments, such NPs were proved to be appropriate probes for XFCT application with the detection limit at a sub-mg/mL level. In the future research, such NPs could be further functionalized with targeting molecules for early-phase cancer detection.

6.
Mol Ther Nucleic Acids ; 18: 16-23, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31479921

RESUMO

Among the large number of known microRNAs (miRNAs), some miRNAs play negligible roles in cell regulation. Therefore, selecting essential miRNAs is an important initial step for a deeper understanding of miRNAs and their functions. In this study, we generated 60 classification models by combining 12 representative feature extraction methods and 5 commonly used classification algorithms. The optimal model for essential miRNA classification that we obtained is based on the Mismatch feature extraction method combined with the random forest algorithm. The F-Measure, area under the curve, and accuracy values of this model were 93.2%, 96.7%, and 93.0%, respectively. We also found that the distribution of the positive and negative examples of the first few features greatly influenced the classification results. The feature extraction methods performed best when the differences between the positive and negative examples were obvious, and this led to better classification of essential miRNAs. Because each classifier's predictions for the same sample may be different, we employed a novel voting method to improve the accuracy of the classification of essential miRNAs. The performance results showed that the best classification results were obtained when five classification models were used in the voting. The five classification models were constructed based on the Mismatch, pseudo-distance structure status pair composition, Subsequence, Kmer, and Triplet feature extraction methods. The voting result was 95.3%. Our results suggest that the voting method can be an important tool for selecting essential miRNAs.

7.
J Agric Food Chem ; 67(35): 9749-9756, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31415718

RESUMO

Bovine lactoferrin N-lobe plays an important key in the nonimmunological defense system. In this work, the most suitable promoter Pveg was selected and the fragment coding bovine lactoferrin N-lobe was optimized according to codon bias of Bacillus. The recombinant plasmid pMA0911-Pveg-mBLF-N was introduced into Baicillus subtilis 168 to create B. subtilis/pMA0911-Pveg-mBLF-N. The bovine lactoferrin N-lobe was highly expressed at 28 °C for 15 h. Its purified protein was obtained with 16.5 mg/L and a purity of 93.6% using ammonium sulfate precipitation, Ni-NTA, and molecular exclusion. About 200 ng/mL purified bovine lactoferrin N-lobe completely inhibited cell-growth of Escherichia coli JM109 (DE3), 70.3% of Pseudomonas aeruginosa CGMCC 1.6740, and 41.5% of Staphylococcus aureus CGMCC 1.282. To our knowledge, this is the first report about active expression, purification, and characterization of bovine lactoferrin N-lobe in safe bacterium B. subtilis, which opens an available application way in the biomedical and food industries.


Assuntos
Antibacterianos/farmacologia , Bacillus subtilis/genética , Códon/genética , Lactoferrina/genética , Regiões Promotoras Genéticas , Animais , Antibacterianos/metabolismo , Bacillus subtilis/metabolismo , Clonagem Molecular , Códon/metabolismo , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Expressão Gênica , Engenharia Genética , Lactoferrina/metabolismo , Lactoferrina/farmacologia , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/crescimento & desenvolvimento , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento
8.
Environ Sci Pollut Res Int ; 26(26): 27023-27031, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31313232

RESUMO

Ammonia is one of the most common aquatic pollutants. To analyze the effect of ammonia exposure on the glutathione redox system, we investigated the levels of hydrogen peroxide (H2O2) and glutathione, and transcription and activities of glutathione-related enzymes in liver and gills of FFRC strain common carp (Cyprinus carpio L.) exposed to 0, 10, 20, and 30 mg/L of ammonia. The results showed that H2O2 content reached a maximum level at 48 h of exposure in the liver of fish. In gills, H2O2 increased rapidly at 6 h and reached to maximum levels at 24 h of exposure, indicating that gills experienced oxidative stress earlier than the liver of fish exposed to ammonia. Reduced glutathione (GSH) content and reduced glutathione/oxidized glutathione (GSH/GSSG) ratio increased significantly within 24 h of exposure. Meanwhile, the transcription and activities of glutathione S-transferase (GST) and glutathione reductase (GR) increased significantly in the liver, and glutathione peroxidase (GSH-Px) and GST increased in the gills of fish exposed to ammonia. Malondialdehyde (MDA) content kept at a low level after exposure to low concentration of ammonia, but increased significantly after exposure to 30 mg/L ammonia for 48 h along with a decrease in GSH content and GSH/GSSG ratio. These data showed that the glutathione redox system played an important role in protection against ammonia-induced oxidative stress in the liver and gills of FFRC strain common carp, though the defense capacity was not able to completely prevent oxidative damage occurring after exposure to higher concentration of ammonia. This research systematically studied the response of the glutathione redox system to ammonia stress and would provide novel information for a better understanding of the adaptive mechanisms of fish to environmental stress.


Assuntos
Amônia/toxicidade , Carpas/metabolismo , Poluentes Químicos da Água/toxicidade , Animais , Ecotoxicologia , Brânquias/efeitos dos fármacos , Brânquias/metabolismo , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Glutationa Redutase/metabolismo , Glutationa Transferase/metabolismo , Peróxido de Hidrogênio/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Malondialdeído/metabolismo , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Testes de Toxicidade Aguda
9.
Molecules ; 24(11)2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31163701

RESUMO

Hsp90 (Heat shock protein 90) is involved in various processes in cancer occurrence and development, and therefore represents a promising drug target for cancer therapy. In this work, a virtual screening strategy was employed, leading to the identification of a series of compounds bearing a scaffold of 1,3-dibenzyl-2-aryl imidazolidine as novel Hsp90 inhibitors. Compound 4a showed the highest binding affinity to Hsp90α (IC50 = 12 nM) in fluorescence polarization (FP) competition assay and the strongest anti-proliferative activity against human breast adenocarcinoma cell line (MCF-7) and human lung epithelial cell line (A549) with IC50 values of 21.58 µM and 31.22 µM, respectively. Western blotting assays revealed that these novel Hsp90 inhibitors significantly down-regulated the expression level of Her2, a client protein of Hsp90, resulting in the cytotoxicity of these novel Hsp90 inhibitors. The molecular docking study showed that these novel Hsp90 inhibitors bound to the adenosine triphosphate (ATP) binding site at the N-terminus of Hsp90. Furthermore, structure-activity relationship studies indicated that the N-benzyl group is important for the anti-cancer activity of 1,3-dibenzyl-2-aryl imidazolidines.


Assuntos
Proteínas de Choque Térmico HSP90/antagonistas & inibidores , Imidazolidinas/química , Imidazolidinas/farmacologia , Células A549 , Proliferação de Células/efeitos dos fármacos , Ensaios Clínicos como Assunto , Avaliação Pré-Clínica de Medicamentos , Proteínas de Choque Térmico HSP90/metabolismo , Humanos , Concentração Inibidora 50 , Células MCF-7 , Simulação de Acoplamento Molecular , Relação Estrutura-Atividade , Interface Usuário-Computador
10.
iScience ; 17: 1-9, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31247446

RESUMO

One-dimensional perovskites are an interesting material for energy and optoelectronic applications. However, exploring the full wealth of architectures these materials could allow, through multi-element doping of A-sites and B-sites, is still a challenge. Here, we report a high-yield synthetic strategy for 1D perovskites via a two-step method based on a multi-element topochemical-molten salt method. Typically, a high yield of 1D multicomponent perovskite niobates (Li0.06Na0.47K0.47)(Nb0.94Sb0.06)O3 (LNKNS2) is rapidly achieved from as-synthesized 1D K2(Nb0.94Sb0.06)8O21 with multi-element B-sites. In this process, 1D K2(Nb0.94Sb0.06)8O21 has been first achieved, and the proportion of the ions in A-sites is affected by the radius and molar ratio of ions. The z axis direction of K2(Nb0.94Sb0.06)8O21 rod is transformed into the x axis direction of LNKNS2 rod. Furthermore, the output voltage of the 1D niobates-based flexible piezoelectric device (FPD) was nearly 600% compared with that of the isotropic niobates-based FPD. This work also allows convenient fabrication of other 1D multicomponent perovskites.

11.
Sensors (Basel) ; 19(12)2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31226761

RESUMO

The second-generation of the Beidou Navigation Satellite System (BDS-2) has been officially providing positioning, navigation, and timing (PNT) services within the Asia-Pacific region for six years, starting from 2013. A comprehensive analysis of BDS-2 satellite broadcast navigation message performance during the past six years is highly demanded, not only for the regional service but also for the global service announced in December 2018. Therefore, this study focuses on the performance assessment of six-year BDS-2 broadcast navigation messages from 2013 to 2018 in three aspects: Message availability, anomaly detection, and signal-in-space user range errors (SIS UREs). Firstly, our results, based on International GNSS service (IGS) Multi-GNSS Experiment (MGEX) navigation files, indicate that the BDS-2 Geosynchronous Earth Orbit (GEO) and Inclined Geosynchronous Satellite Orbit (IGSO) satellites have >98.51% broadcast navigation message availability, and the Medium Earth Orbit (MEO) satellites has a ~90.03% availability. Secondly, the comparison between broadcast navigation messages and IGS precise products reveals that the User Range Accuracy Index (URAI) contained in the broadcast message could not reflect satellite performance correctly. Another satellite status indicator, space vehicle (SV) health, can only partially detect a satellite anomaly. The anomaly detection result using IGS precise products for reference shows 20241 anomalies out of 651038 broadcast navigation messages within six years. Finally, compared with the IGSO and MEO satellites, the orbit qualities of GEO satellites are significantly worse due to their large along-track orbit error. The clock performance of all satellites are at the comparable level. The satellite orbit type (GEO/IGSO/MEO) does not impact the orbit-only URE, global-average URE, and worst-case URE.

12.
Zhen Ci Yan Jiu ; 44(4): 276-81, 2019 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-31056881

RESUMO

OBJECTIVE: To observe the effect of acupoint injection on expression of fork head/winged helix protein 3 (Foxp3), retinoic acid-related orphan receptor γt (RORγt) in nasal mucosa and serum interleukin-17 (IL-17) level in allergic rhinitis (AR) rats, so as to explore its mechanism underlying improvement of AR in terms of balancing Th17/Treg. METHODS: Thirty-two SD rats (half male and half female) were randomized into normal control, AR model, acupoint injection and non-acupoint injection groups (n=8 in each group). The AR model was established by ovalbumin sensitization. In the acupoint injection group, "Yintang" (EX-HN3) and bilateral "Yingxiang"(LI20) were selected for injection of mixture solution of dexamethasone (DEX) and transfer factor and lidocaine (0.1 mL/acupoint), once every 3 days for a total of 4 times. The non-acupoints, located at the mid-point between the "Houhai" (GV1) and "Huantiao"(GB30) on the bilateral hips and the sites 5 cm inferior to the axillary were injected with the same dose of mixture solution as that in the acupoint injection. The AR severity was assessed by cumulative quantification scoring methods (including the numbers of nose-catching and sneezes, and the amount of nasal secretions in 30 min). The expressions of Foxp3 and RORγt in the nasal mucosa were detected by immunohistochemistry. The serum IL-17 content was detected by enzyme linked immunosorbent assay (ELISA).. RESULTS: The AR symptom score and serum IL-17 content were significantly higher in the AR model group than those in the normal control group (P<0.05), and significantly down-regulated in the acupoint injection group (not in the non-acupoint group) relevant to the AR model group (P<0.05). Following modeling, the expression levels of nasal Foxp3 protein was significantly down-regulated while that of RORγt protein markedly up-regulated in the AR model group relevant to the normal control group (P<0.05), indicating an imbalance between Foxp3 and RORγt activity(P<0.05). After EA intervention, the increased expression of Foxp3 and the down-regulated expression of RORγt were revised in the acupoint injection group (P<0.05) but not in the non-acupoint group (P>0.05). The percentage of the Foxp3 positive cells and the ratio of Foxp3/RORγt were negatively correlated with the AR symptom score(P<0.05), the expression of RORγt and the content of IL-17 were positively correlated with the symptom score (P<0.05).. CONCLUSION: Acupoint injection is able to improve symptoms of RA rats, which may be related with its function in up-regulating the level of nasal mucosal Foxp3 and suppressing the levels of nasal RORγt and serum IL-17 to correct the imbalance of Th17/Treg.


Assuntos
Rinite Alérgica , Linfócitos T Reguladores , Pontos de Acupuntura , Animais , Feminino , Masculino , Ratos , Ratos Sprague-Dawley , Células Th17
13.
Pharmacotherapy ; 39(7): 783-789, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31069819

RESUMO

Chronic subdural hematoma (CSDH) can develop in children in rare cases. Burr-hole drainage (BHD) is the treatment of choice, but it is associated with a high rate of recurrence. This report describes four cases of pediatric patients (1-7 yrs of age) with post-BHD relapsed CSDH who were successfully treated with a drug regimen that included 2.5-5 mg atorvastatin daily combined with dexamethasone with stepwise-decreasing dosing for a total of 4 weeks. After 4 weeks of treatment, the hematoma was completely resolved in three patients and significantly reduced in one patient. During the treatment, no patient reported clinically significant adverse events. No patient experienced hematoma relapse during the follow-up period that lasted for up to 4 years. This case report suggests the need for a randomized placebo-controlled trial to evaluate this drug regimen for nonsurgical treatment of patients with relapsed CSDH.

14.
Mol Cell Probes ; 45: 57-64, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31096000

RESUMO

AIM: Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS: THP1 cells were induced to polarized macrophages (MΦ). A549 and H1795 cells were separately treated with MΦ conditioned medium, ES (12.5 µg/ml) and HGF (5 ng/ml) for 24 h at 37 °C. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression levels of CCL17, CD163, hepatocyte growth factor (HGF), Epidermal Growth Factor (EGF), transforming growth factor (TGF)-ß1 and interleukin (IL)-6. Western blot was carried out to detect the p-MET, MET and EMT-related proteins (E-cadherin, N-cadherin, Snail and vimentin). Fibroblast-like A549 and H1975 cells were observed by a microscope. Cell invasion and migration were observed and analyzed by transwell and scratch assays. RESULTS: The expression levels of CCL17 and CD163 were significant higher in MΦ. ES significantly inhibited the expression of HGF in MΦ. Moreover, ES could restore the abnormal expressions of EMT-related proteins and inhibit MΦ-induced and HGF-induced fibroblast-like lung cancer cells. Furthermore, ES suppressed the MΦ-induced and HGF-induced migration and invasion of lung cancer cells. ES was also found to down-regulate HGF-Met signaling in HGF-treated lung cancer cells. CONCLUSION: ES suppresses lung cancer progression by down-regulating HGF-Met signaling, revealing the possible mechanism of ES in the process of treating lung cancer patients.

15.
J Transl Med ; 17(1): 177, 2019 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-31133033

RESUMO

BACKGROUND: Kidney renal clear cell carcinoma (KIRC) is the most common type of kidney cancer in adults, and it is responsible for approximately 90-95% of cases. Although extensive evidence has suggested that many immune- and inflammation-related genes could serve as effective biomarkers in KIRC, the potential associations among immune-, inflammation- and KIRC-related genes has not been sufficiently understood. METHODS: Here, we integrated multiple levels of data to construct an immune-, inflammation- or KIRC-directed neighbour network (IIKDN network) and a KIRC-related gene-directed network (KIRCD network). RESULTS: Our analysis suggested that immune- and inflammation-related genes in the network have special topological characteristics and expression patterns related to KIRC. We further identified five core clusters that showed a tighter network structure and stronger correlation of expression from the KIRCD network. Specifically, multiple-level molecular characteristics were systematically portrayed, including somatic mutation, copy-number variant and DNA methylation for the genes in five core clusters. We discovered that the genes showed strong correlation with respect to the expression and methylation levels in these five core clusters. These five core clusters could become special prognostic biomarkers for KIRC, and functional analysis showed that they were associated with activation of the immune and inflammation systems and cancer progression. CONCLUSIONS: Our findings highlighted the novel role of the immune and inflammation genes in KIRC.

16.
J Proteome Res ; 18(7): 2931-2939, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31136183

RESUMO

Cellular respiration provides direct energy substances for living organisms. Electron storage and transportation should be completed through electron transport chains during the cellular respiration process. Thus, identifying electron transport proteins is an important research task. In protein identification, selection of the feature extraction method and classification algorithm has a direct bearing on classification. The distance-based Top-n-gram method, which was proposed based on the frequency profile and considered evolutionary information, was used in this study for feature extraction. The Max-Relevance-Max-Distance algorithm was adopted for feature selection. The first 4D features that greatly influenced the classification result were selected to form the feature data set. Finally, the random forest algorithm was used to identify electron transport proteins. Under the 10-fold cross-validation of the model constructed in this study, sensitivity, specificity, and accuracy rates surpassed 85%, 80%, and 82%, respectively. In the testing set, F-measure, AUC value, and accuracy exceeded 74%, 95%, and 86%, respectively. These experimental results indicated that the classification model built in this study is an effective tool in identifying electron transport proteins.

17.
Int J Pharm ; 564: 22-38, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31002933

RESUMO

AIM: The current study reports the development and evaluation of chitosan-sericin-silver nanocomposite (CSSN) films without and with moxifloxacin (Mox). METHODOLOGY: The film preparation method involved the in-situ synthesis of silver nanoparticles within the chitosan-sericin colloidal composite followed by preparation into a film by solvent casting technique. In-situ formation and the particle size analysis of the silver nanoparticles was performed via UV-Visible and zeta-size spectrometer. The prepared films were tested for swelling ratio, contents uniformity, in-vitro Mox release, and permeation analysis. The morphological (SEM), elemental (EDX), spectral (FT-IR), structural (XRD), and thermal (TGA and DSC) properties of the composites were also inspected. The antibacterial activity of the CSSN films was performed against seven pathogenic bacterial strains including five ATCC and two clinical strains. The potential wound healing activity of the composite films was evaluated on burn wound model induced in Sprague Dawley male rats. RESULTS: The prepared films displayed good swelling profile with a sustained in-vitro Mox release and permeation profile; attaining maximum of 78.57% (CSSM3) release and 55.05% (CSSM1) permeation (CSSM1) in 24 h. The prepared films, particularly the Mox-loaded CSSN films displayed a promising antibacterial activity against all the tested strains with the activity being highest against MRSA (clinical isolates). The prepared films indicated a remarkable wound healing applications with successful fibrosis, collagen reorganization, neovascularization, and mild epidermal regeneration after 7 days of treatment with no silver ions detection in animal's blood. CONCLUSION: The obtained findings strongly suggest the use of the prepared novel composite dressing for wound care applications.


Assuntos
Antibacterianos/administração & dosagem , Quitosana/administração & dosagem , Moxifloxacina/administração & dosagem , Nanocompostos/administração & dosagem , Sericinas/administração & dosagem , Prata/administração & dosagem , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Bandagens , Queimaduras/tratamento farmacológico , Queimaduras/patologia , Quitosana/química , Liberação Controlada de Fármacos , Masculino , Moxifloxacina/química , Nanocompostos/química , Ratos Sprague-Dawley , Sericinas/química , Prata/química , Pele/efeitos dos fármacos , Pele/patologia , Absorção Cutânea
18.
Front Microbiol ; 10: 507, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30972038

RESUMO

The uniqueness of bacteriophages plays an important role in bioinformatics research. In real applications, the function of the bacteriophage virion proteins is the main area of interest. Therefore, it is very important to classify bacteriophage virion proteins and non-phage virion proteins accurately. Extracting comprehensive and effective sequence features from proteins plays a vital role in protein classification. In order to more fully represent protein information, this paper is more comprehensive and effective by combining the features extracted by the feature information representation algorithm based on sequence information (CCPA) and the feature representation algorithm based on sequence and structure information. After extracting features, the Max-Relevance-Max-Distance (MRMD) algorithm is used to select the optimal feature set with the strongest correlation between class labels and low redundancy between features. Given the randomness of the samples selected by the random forest classification algorithm and the randomness features for producing each node variable, a random forest method is employed to perform 10-fold cross-validation on the bacteriophage protein classification. The accuracy of this model is as high as 93.5% in the classification of phage proteins in this study. This study also found that, among the eight physicochemical properties considered, the charge property has the greatest impact on the classification of bacteriophage proteins These results indicate that the model discussed in this paper is an important tool in bacteriophage protein research.

19.
Zhen Ci Yan Jiu ; 44(2): 148-52, 2019 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-30945494

RESUMO

OBJECTIVE: To observe the time-effect of stimulation of the embedded poly glycolide-co-lactide (PGLA) suture in Sanyinjiao (SP6) area in normal human body, so as to provide an experimental evidence for clinical application of micro-invasion suture-embedding at an appropriate interval. METHODS: A total of 8 healthy volunteer students (3 boys and 5 girls, ranging in age from 24 to 27 years) were recruited in the present study. A piece of sterilized PGLA suture was implanted into the left SP6 using minimally invasive surgery after strict local skin disinfection. The fat-suppression T2 weighted magnetic resonance images (MRI, displaying local lesion after eliminating interference of fat tissue signals), and T2 mapping 8-echo train images were acquired before and 8 h, 3, 7, 10 and 14 days after PGLA suture embedment by using a MR imaging system. After transformation of the T2-mapping 8-echo train images into T2-mapping images by using a relevant software, the T2 values (meaning the relaxation time of the local muscle) of the left SP6 were measured, followed by analysis of the signal intensity of T2 weighted fat-suppression images and T2 values at different time-points. RESULTS: Before the suture embedding, no abnormal signals were found in the signal intensity of T2 weighted fat-suppression images. After PGLA suture embedment, the local signal intensity of T2WI fat-suppression images was relatively increased at the 8th h, and on day 3, 7, 10 and 14 relevant to pre-embedment, but gradually atte-nuated on day 10 and 14. The T2 values were significantly increased at the 5 time-points of post-embedment (all P<0.01), but without significant differences among the 8thh, the 3rd and 7thd (P>0.05), and being markedly lowered on day 14 relevant to day 7 (P<0.01) in spite of being still markedly higher than that of pre-embedding (P<0.01). CONCLUSION: The signal intensity of T2 weighted fat-suppression images and T2 values acquired from PGLA-suture-embedded SP6 acupoint area in healthy subjects may keep at least for 2 weeks, suggesting that the stimulating reaction of suture-embedment persists more than 14 days. Hence, when a micro-invasion embedding with PGLA suture performed, the interval of two weeks would be appropriate.


Assuntos
Imagem por Ressonância Magnética , Suturas , Adulto , Dioxanos , Feminino , Voluntários Saudáveis , Humanos , Masculino , Adulto Jovem
20.
Zhongguo Zhen Jiu ; 39(4): 391-5, 2019 Apr 12.
Artigo em Chinês | MEDLINE | ID: mdl-30957450

RESUMO

OBJECTIVE: To explore the objectivity and time-effect of stimulating effect at acupoint with PGLA in the healthy person, and to provide a basis for the rational interval of minimally invasive embedding of PGLA. METHODS: Before embedding, 8 h, 3rd, 7th, 10th, 14th day after embedding, medical imaging magnetic resonance imaging (MRI) scanning technique was used to collect local T2WI pressure-lowering and T2-Mapping 8 echoes sequence image of left Zusanli (ST 36) in 8 cases of healthy person. The T2-Mapping 8 echoes sequence image was generated by the relevant software to the T2-Mapping image and the local T2 value was measured. The characteristics of local T2WI pressure-fat image signal intensity and the change of T2 value at left Zusanli (ST 36) with minimally invasive embedding with PGLA were observed and analyzed. RESULTS: ①There was no abnormal signal on the T2WI pressure-fat image on the left Zusanli (ST 36) point before the embedding. The high-signal was seen on the local T2WI pressure-fat image at each time point after embedding, there was no significant difference in local signal intensity between 8 h, 3rd and 7th day after embedding. The local signal intensity decreased on the 10th day after embedding, and the local signal intensity decreased significantly on the 14th day after embedding.②The T2 value at each time point after embedding increased significantly compared with that before embedding (all P<0.01); there was no significant difference in T2 value among the 8 h, 3rd and 7th day after embedding (all P>0.05); there was no significant difference between the T2 value on the 7th and the 10th day after embedding (P>0.05),the T2 value on the 14th day after embedding was significantly lower than that on the 7th day after embedding (P<0.01). CONCLUSION: It has a stimulating effect on the local acupoints with minimally invasive embedding with PGLA in the healthy person, and the stimulating effect has certain time-effect. The effective stimulation time is about 2 weeks. The rational interval period for the minimally invasive embedding with the PGLA of the same specification type should be about 2 weeks.


Assuntos
Pontos de Acupuntura , Imagem por Ressonância Magnética , Peptídeos Catiônicos Antimicrobianos
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