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Adv Clin Exp Med ; 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31782272


BACKGROUND: Rapamycin inhibits the mammalian target of rapamycin (mTOR) activity and has been proven effective for the treatment of lung injury. OBJECTIVES: The objective of this study was to investigate the roles of the mTOR pathway and its inhibitor rapamycin in the repair of hyperoxia-induced acute lung injury (ALI). MATERIAL AND METHODS: Firstly, premature rat lung fibroblast L929 cells were cultured under different oxygen concentrations (40%, 60%, and 90%). At day 3, 7 and 14 after exposure, MTT assay and flow cytometry were used to evaluate the effect of oxygen stress on cell viability and apoptosis of L929 cells, respectively. Secondly, microscopy, MTT assay and flow cytometry was used to investigate the effect of 10 nM rapamycin on 90% O2 exposed L929 cells. We also used small interfering RNAs (siRNAs) to abrogate the expression of mTOR in 90% O2 exposed L929 cells, and then evaluated the apoptosis and cell viability using flow cytometry and the MTT assay, respectively. In addition, western blot was used to detect the protein expression of Bcl-2, p53, TGF-ß and connective tissue growth factor (CTGF). A hyperoxia-induced lung injury model was established in Sprague Dawley (SD) rats in order to evaluate the histopathological changes in lung tissues and expression of the mTOR pathway and fibrosis related factors. RESULTS: Exposure to 40%, 60% or 90% oxygen all significantly inhibited the growth of L929 cells. Application of 10 nM rapamycin was found to effectively promote apoptosis of 90% O2 exposed L929 cells. In addition, mTOR siRNA promoted the apoptosis and inhibited the growth of L929 cells. Rapamycin inhibited the activation of the mTOR signaling pathway, down-regulated the expression of downstream proteins p70S6K and 4EBP1, reduced the collagen deposition and the production of fibrosis-inducing factors, including TGF-ß and CTGF in hyperoxia-induced lung injury rats. CONCLUSIONS: Rapamycin may be useful for the treatment of hyperoxia-induced acute lung injury (ALI) by inhibiting the activation of mTOR signaling pathway.

J Addict Med ; 8(3): 183-8, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24695019


OBJECTIVES: Long-term use of methamphetamine (MA) induces the neuron damage and leads to multiple neuropsychiatric disorders. However, the effect of MA on the female reproductive functions has not yet been evaluated. The objective of this study was to determine the prevalence of abnormal menstrual cycling in female MA users. METHODS: Female MA users (N = 113) were recruited from the Zhangjiang Isolated Compulsory Detoxification Center. Gynecologic history and drug use history were recorded, and serum levels of follicle-stimulating hormone, luteinizing hormone, prolactin, estrogen, progesterone, and testosterone were measured. RESULTS: Long-term use of MA significantly altered the menstrual cycle, and 33.6% women suffered from abnormal uterine bleeding while using MA. Deregulation of sex hormones was observed in 73.3% of participants during abstinence. The most common patterns were simple anovular menstruation, which was caused mainly by a hypothalamic deregulation and pituitary suppression with or without ovarian suppression. Normal hormone levels were observed more frequently in participants abstinent for more than 10 months (39.5%) than in participants who were abstinent for less than 10 months (18.6%). However, no relationship was found between hormone deregulation and age or history of MA use. CONCLUSIONS: The present data demonstrate that long-term use of MA results in the disruption of menstrual cycles and dysfunction of hypothalamic-pituitary-gonadal axis in women.

Transtornos Relacionados ao Uso de Anfetaminas/complicações , Estimulantes do Sistema Nervoso Central/efeitos adversos , Sistema Hipotálamo-Hipofisário/efeitos dos fármacos , Ciclo Menstrual/efeitos dos fármacos , Metanfetamina/efeitos adversos , Ovário/efeitos dos fármacos , Adulto , Transtornos Relacionados ao Uso de Anfetaminas/sangue , Transtornos Relacionados ao Uso de Anfetaminas/fisiopatologia , Estrogênios/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Humanos , Sistema Hipotálamo-Hipofisário/fisiopatologia , Hormônio Luteinizante/sangue , Distúrbios Menstruais/sangue , Distúrbios Menstruais/induzido quimicamente , Distúrbios Menstruais/fisiopatologia , Ovário/fisiopatologia , Progesterona/sangue , Prolactina/sangue , Testosterona/sangue , Adulto Jovem
Artigo em Chinês | MEDLINE | ID: mdl-14768079


OBJECTIVE: To estimate clinical effect of spin iliac deep vascular pedicled periosteum flap in repairing traumatic femoral neck of theca inside fracture in young and middle-aged. METHODS: From April 1993 to September 2001, 12 cases of traumatic femoral neck fracture were given diaplastic operation with fixation of 3 centre hollow pressed bolt and were conducted under os traction bed and "C" arm X-ray machine. Spin iliac deep vascular pedicled periosteum flap was stripped off, and transferred to the front of femoral neck fundus, then transplanted to the narrow inside of fracture through outer open door of articular capsule. RESULTS: All patients were followed up for 1-7 years. All fracture healed without femoral head necrosis, but mild arthritis appeared in 7 cases. CONCLUSION: Vascular pedicled periosteum flap transfer of young and middle-aged femoral neck fracture, by decompression of femoral neck and reconstruction of blood circulation, can promote the fracture healing and decrease the wound and blood circulation destroy.

Fraturas do Colo Femoral/cirurgia , Fixação Interna de Fraturas/métodos , Periósteo/transplante , Retalhos Cirúrgicos , Adulto , Feminino , Fraturas do Colo Femoral/diagnóstico por imagem , Fraturas do Colo Femoral/patologia , Consolidação da Fratura , Humanos , Ílio/irrigação sanguínea , Ílio/transplante , Masculino , Pessoa de Meia-Idade , Periósteo/irrigação sanguínea , Radiografia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Retalhos Cirúrgicos/irrigação sanguínea