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1.
J Sci Food Agric ; 100(1): 161-167, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31471904

RESUMO

BACKGROUND: Rapid and accurate diagnosis of nitrogen (N) status in field crops is of great significance for site-specific N fertilizer management. This study aimed to evaluate the potential of hyperspectral imaging coupled with chemometrics for the qualitative and quantitative diagnosis of N status in tea plants under field conditions. RESULTS: Hyperspectral data from mature leaves of tea plants with different N application rates were preprocessed by standard normal variate (SNV). Partial least squares discriminative analysis (PLS-DA) and least squares-support vector machines (LS-SVM) were used for the classification of different N status. Furthermore, partial least squares regression (PLSR) was used for the prediction of N content. The results showed that the LS-SVM model yielded better performance with correct classification rates of 82% and 92% in prediction sets for the diagnosis of different N application rates and N status, respectively. The PLSR model for leaf N content (LNC) showed excellent performance, with correlation coefficients of 0.924, root mean square error of 0.209, and residual predictive deviation of 2.686 in the prediction set. In addition, the important wavebands of the PLSR model were interpreted based on regression coefficients. CONCLUSION: Overall, our results suggest that the hyperspectral imaging technique can be an effective and accurate tool for qualitative and quantitative diagnosis of N status in tea plants. © 2019 Society of Chemical Industry.

2.
Microbiol Res ; 230: 126343, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31539852

RESUMO

Identifying the direct target genes of response regulators (RRs) of a bacterial two-component system (TCS) is critical to understand the roles of TCS in bacterial environmental adaption and pathogenesis. Actinobacillus pleuropneumoniae is an important respiratory bacterial pathogen that causes considerable economic losses to swine industry worldwide. The targets of A. pleuropneumoniae NarP (nitrate/nitrite RR), which is the cognate RR of the nitrate/nitrite sensor histidine kinase NarQ, are still unknown. In the present study, a DNA-affinity-purified sequencing (DAP-Seq) approach was established. The upstream regions of a total of 131 candidate genes from the genome of A. pleuropneumoniae were co-purified with the activated NarP protein. Electrophoretic mobility shift assay (EMSA) results confirmed the interactions of NarP with the promoter regions of five selected target genes, including dmsA, pgaA, ftpA, cstA and ushA. The EMSA-confirmed target genes were significantly up-regulated in the narP-deleted mutant in the presence of additional nitrate, whilst the transcriptional changes were restored in the complemented strain. The NarP binding motif in the upstream regions of the target genes dmsA and ftpA were further identified and confirmed by EMSA using the truncated binding motif. The NarP binding sites were present in a total of 25.2% of the DNA fragments captured by DAP-Seq. These results demonstrated that the established DAP-Seq method is effective for exploring the direct targets of RRs of bacterial TCSs and that the A. pleuropneumoniae NarP could be a repressor in response to nitrate.

3.
Sci Total Environ ; 699: 134370, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31678883

RESUMO

Synthetic industrial wastewater containing approximately 2000 mg/L N, N-dimethylformamide (DMF) was treated using a lab-scale anaerobic sludge blanket (UASB) and an anaerobic membrane bioreactor (AnMBR) in this study. The inoculum consisted of two sources of sludge: Co-culture of anaerobic digested sludge (ADS) with DMF-hydrolyzing activated sludge (DAS) for the AnMBR, and co-culture of anaerobic granular sludge (AGS) with DAS for the UASB. Effective DMF methanogenic degradation of nearly 100% removal was achieved in both reactors on the first day. Both reactors obtained excellent DMF removal efficiency and high methane production under a low organic loading rate (OLR) of around 3-4 g COD/L/d. However, excessive elevation of OLR significantly limited DMF hydrolysis. When OLR exceeded 6 g COD/L/d, the removal efficiency and methane production in both reactors dramatically dropped. Despite their different forms and shapes, the ADS and AGS both provide methanogens which are responsible for methanogenesis. The UASB tolerated a higher OLR while the AnMBR was limited by membrane fouling due to the increased sludge concentration. However, the AnMBR obtained high-quality effluent without suspended solid. Whether DMF can be effectively degraded depends on DAS, in which abundant DMF-hydrolyzing bacteria (DHB) provide sufficient quantities of the hydrolytic enzyme for effective hydrolysis of DMF. However, these DHB were facultative and were also identified as denitrifying bacteria which require nitrate as the electron acceptor or otherwise survive under the aerobic condition. They gradually decayed rather than proliferated and were outcompeted by methanogens. Therefore, it is conceivable that a slight dosage of nitrate would enrich the abundance of DHB in both the UASB and the AnMBR, and provide a sufficient quantity of enzymes for the DMF hydrolysis. The cultivation of the anaerobic DMF-degrading granular sludge using the UASB is considered an upgraded solution to the effective treatment of DMF-containing wastewater.

4.
Nucleic Acids Res ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31701150

RESUMO

Computational modelling has become increasingly common in life science research. To provide a platform to support universal sharing, easy accessibility and model reproducibility, BioModels (https://www.ebi.ac.uk/biomodels/), a repository for mathematical models, was established in 2005. The current BioModels platform allows submission of models encoded in diverse modelling formats, including SBML, CellML, PharmML, COMBINE archive, MATLAB, Mathematica, R, Python or C++. The models submitted to BioModels are curated to verify the computational representation of the biological process and the reproducibility of the simulation results in the reference publication. The curation also involves encoding models in standard formats and annotation with controlled vocabularies following MIRIAM (minimal information required in the annotation of biochemical models) guidelines. BioModels now accepts large-scale submission of auto-generated computational models. With gradual growth in content over 15 years, BioModels currently hosts about 2000 models from the published literature. With about 800 curated models, BioModels has become the world's largest repository of curated models and emerged as the third most used data resource after PubMed and Google Scholar among the scientists who use modelling in their research. Thus, BioModels benefits modellers by providing access to reliable and semantically enriched curated models in standard formats that are easy to share, reproduce and reuse.

5.
BMC Oral Health ; 19(1): 246, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722703

RESUMO

BACKGROUND: The extent to which the composition and diversity of the oral microbiome varies with age is not clearly understood. METHODS: The 16S rRNA gene of subgingival plaque in 1219 women, aged 53-81 years, was sequenced and its taxonomy annotated against the Human Oral Microbiome Database (v.14.5). Composition of the subgingival microbiome was described in terms of centered log(2)-ratio (CLR) transformed OTU values, relative abundance, and prevalence. Correlations between microbiota abundance and age were evelauted using Pearson Product Moment correlations. P-values were corrected for multiple testing using the Bonferroni method. RESULTS: Of the 267 species identified overall, Veillonella dispar was the most abundant bacteria when described by CLR OTU (mean 8.3) or relative abundance (mean 8.9%); whereas Streptococcus oralis, Veillonella dispar and Veillonella parvula were most prevalent (100%, all) when described as being present at any amount. Linear correlations between age and several CLR OTUs (Pearson r = - 0.18 to 0.18), of which 82 (31%) achieved statistical significance (P < 0.05). The correlations lost significance following Bonferroni correction. Twelve species that differed across age groups (each corrected P < 0.05); 5 (42%) were higher in women ages 50-59 compared to ≥70 (corrected P < 0.05), and 7 (48%) were higher in women 70 years and older. CONCLUSIONS: We identified associations between several bacterial species and age across the age range of postmenopausal women studied. Understanding the functions of these bacteria could identify intervention targets to enhance oral health in later life.

6.
Reprod Biol Endocrinol ; 17(1): 96, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31744497

RESUMO

BACKGROUND: Previous work demonstrated that there are numerous miRNAs in human follicular fluids, some of which are associated with reproductive diseases. In the current study, we sought to determine whether microRNAs (miRNAs) in the follicular fluid (FF) are differentially expressed between women with and without endometriosis and to uncover the association of miRNAs with the oocyte and embryonic development potential. METHODS: FF was harvested from 30 women with endometriosis and 30 women without who underwent in vitro fertilization treatment at the University Hospital between February and December 2016. The FF samples were subjected to miRNA profiling and validation via quantitative reverse transcription polymerase chain reaction analysis. Mouse/human metaphase-I (MI) oocytes were harvested and micro-injected with an miR-451 inhibitor, and the effects of miR-451 knockdown on Wnt/WNT signalling genes were investigated. RESULTS: Oocyte number, fertilization rate, and number of available embryos were decreased significantly in women with endometriosis relative to those without endometriosis. Hsa-miR-451 in FF was downregulated in endometriosis patients relative to control subjects (P < 0.01). Moreover, the proportions of mouse/human MI oocytes that developed into 2-pronuclei (2PN), 2-cell, 8-10-cell and blastocyst-stage embryos were affected by miR-451 knockdown in mouse/human oocytes. Components of the Wnt signalling pathway were aberrantly expressed in the mouse/human oocytes and embryos in the miR-451 inhibitor-injected group. CONCLUSIONS: miR-451 was downregulated in FF samples from endometriosis patients and was modestly effective in distinguishing endometriosis patients from non-endometriosis patients. miR-451 downregulation in mouse and human oocytes affected pre-implantation embryogenesis by suppressing the Wnt signalling pathway. This miRNA might serve as a novel biomarker of oocyte and embryo quality in assisted reproductive treatment.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31746925

RESUMO

In this work, we report a novel strategy for fabricating a luminescent 2D nanocomposite at room temperature by in situ generation of luminescent copper nanoclusters (Cu NCs) embedded in natural monolithic eggshell membrane (ESM) using dithiothreitol as the reducing and capping agent. The established fabrication is facile, cost-effective and viable. The as-prepared Cu NC/ESM nanocomposite exhibited excellent photoluminescence performance, improved chemical, thermal and photo stability, convenient tailoring and flexibility. Significantly, the nanocomposites could be employed as test strips for the visual detection of Ag+ ions based on the luminescence quenching phenomenon and as color conversion layers in light-emitting diodes. Furthermore, application of the proposed strategy for surface luminescence patterning was well demonstrated, indicating great potential in biomass based anti-counterfeiting, information encryption and security paper or sheets.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31680040

RESUMO

A novel and highly selective fluorescent 1,8-naphthalimide-based probe, 3, was designed and synthesized for rapid Cu2+ detection in a CH3CN-H2O (3:1, v/v, pH = 7.4) solution by means of a distinct hydrolysis mechanism via its Cu2+-promoting feature. Upon treatment with Cu2+, the fluorescence response of probe 3 at 550 nm abruptly decreased, which was visible to the naked eye, and this response was accompanied by a clear change of the color of the solution; the color changed from the original yellow color to colorless. This color change occurred due to the Cu2+-promoted hydrolysis of 3, which yielded a fluorescence-quenched product. It is inspiring that probe 3 exhibited excellent sensitivity, a short response time and strong anti-interference recognition. Compared with the allowable amount of Cu2+ (∼20 µM) in drinking water, the detection limit of 3 for Cu2+ is calculated to be 9.15 nM, which is much lower than the amount defined by standards. The probe can be successfully applied for the determination of Cu2+ in real aqueous samples. Furthermore, probe 3 can be used as a fluorescent sensor to detect Cu2+ in biological environments, demonstrating its low toxicity to organisms and good cell permeability in live cell imaging.

9.
J Cell Biochem ; 2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31680325

RESUMO

The apoptosis of human periodontal ligament cells (HPDLCs) may be an important factor of the negative effect of advanced glycation end products (AGEs) on the periodontal tissue of diabetic patients. However, the pathways or potential effects of apoptosis in AGEs-treated HPDLCs have not been fully elucidated. Autophagy is closely related to apoptosis. Herein, we investigated the potential mechanism of apoptosis and autophagy in HPDLCs treated with AGEs via an in vitro model. We found that AGEs-treated HPDLCs showed a time- and concentration-dependent reduction in the cell survival rate. The mitochondrial-dependent apoptosis was induced in AGEs-treated HPDLCs, as confirmed by the mitochondrial membrane potential depolarization, decreased Bcl-2 expression, increased Bax expression, and increased caspase-3 and PARP cleavage. Autophagy was also induced in AGEs-treated HPDLCs, as indicated by the conversion of LC3-II/LC3-I and the presence of autophagosomes. Interestingly, our study results suggested that apoptosis and autophagy were related to reactive oxygen species (ROS) production. In addition, AGEs-induced autophagy acted as a latent factor in decreasing the generation of ROS in HPDLCs and protecting against the AGEs-induced apoptosis. In summary, our study shows that ROS are essential in AGEs-induced HPDLCs apoptosis and autophagy, which may be a molecular mechanism for the repairment of ROS-induced damage in HPDLCs treated with AGEs to promote cell survival. The present study might provide new insights into the therapeutic targeting of HPDLCs autophagy, which could be an additional strategy for periodontitis in patients with diabetes mellitus.

10.
Bioorg Chem ; : 103360, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31668462

RESUMO

The bioactive chemical constituents of Euphorbia ebracteolata have been investigated in the present work using various techniques. On the basis of chromatographic methods, such as silica gel, RP C-18 column chromatography, five novel rosane type diterpenoids with an aromatic A-ring (1-5) have been isolated from the roots of Euphorbia ebracteolata. Their structures were elucidated by widely spectroscopic data, including HRESI-MS, 1D and 2D NMR. Additionally, the inhibitory effects on lipase of these isolated diterpenoids were evaluated in vitro. Compound 1 as a new diterpenoid displayed significant inhibitory effect on lipase (IC50 = 1.0 µM). And, the inhibitory kinetics has been studied fully, which determined a competitive inhibition model for compound 1 on the enzymatic activity of lipase (Ki = 1.8 µM).

11.
Arthritis Res Ther ; 21(1): 230, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31706344

RESUMO

BACKGROUND: The response to treatment for juvenile idiopathic arthritis (JIA) can be staged using clinical features. However, objective laboratory biomarkers of remission are still lacking. In this study, we used machine learning to predict JIA activity from transcriptomes from peripheral blood mononuclear cells (PBMCs). We included samples from children with Native American ancestry to determine whether the model maintained validity in an ethnically heterogeneous population. METHODS: Our dataset consisted of 50 samples, 23 from children in remission and 27 from children with an active disease on therapy. Nine of these samples were from children with mixed European/Native American ancestry. We used 4 different machine learning methods to create predictive models in 2 populations: the whole dataset and then the samples from children with exclusively European ancestry. RESULTS: In both populations, models were able to predict JIA status well, with training accuracies > 74% and testing accuracies > 78%. Performance was better in the whole dataset model. We note a high degree of overlap between genes identified in both populations. Using ingenuity pathway analysis, genes from the whole dataset associated with cell-to-cell signaling and interactions, cell morphology, organismal injury and abnormalities, and protein synthesis. CONCLUSIONS: This study demonstrates it is feasible to use machine learning in conjunction with RNA sequencing of PBMCs to predict JIA stage. Thus, developing objective biomarkers from easy to obtain clinical samples remains an achievable goal.

12.
Int J Biol Macromol ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31726162

RESUMO

Polysaccharide from Grifola frondosa is an excellent metal-ion chelating agent owing to its distinctive structure and outstanding functional activities. Our previous research has successfully synthesized novel organic chromium derived from the chelation ofG. frondosapolysaccharide-chromium (III) [GFP-Cr(III)]. The purpose of present research was to reveal the hypoglycemic and hypolipidemic mechanism of GFP-Cr(III), and its relationship with the modulation of intestinal microflora. Successful fabrication of GFP-Cr(III) was verified by thermogravimetric analysis (TGA), powder X-ray diffraction (XRD) and 1H NMR spectrum.The hypoglycemic and hypolipidemic effects were examined using type 2 diabetic mice induced by a high-fat diet (HFD) and streptozocin (STZ). Results indicated that GFP-Cr(III) intervention improved abnormal serum biochemical indicators (triglyceride (TG), cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and glucose), inhibited lipid accumulation and steatosis in the liver. Metagenomic analysis revealed that GFP-Cr(III) treatment produced obvious changes on the intestinal microflora in T2DM mice. Thecorrelationnetwork analysis further revealed that the serum and hepatic lipid profiles were positively correlated with Streptococcus and Enterococcus, but negatively correlated with Enterorhabdus, Ruminococcaceae-UCG-011, Coriobacteriaceae and Micrococcaceae. Meanwhile, oral administration with GFP-Cr(III) regulated the mRNA expression related to glucose and lipid metabolism. These results of present study suggest that GFP-Cr(III) could be used as potential functional food ingredients for the amelioration of hyperglycemia and hyperlipidemia.

13.
Molecules ; 24(22)2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31726738

RESUMO

Indigo naturalis (IN) is a traditional Chinese medicine, named Qing-Dai, which is extracted from indigo plants and has been used to treat patients with inflammatory bowel disease (IBD) in China and Japan. Though there are notable effects of IN on colitis, the mechanisms remain elusive. Regarding the significance of alterations of intestinal flora related to IBD and the poor water solubility of the blue IN powder, we predicted that the protective action of IN on colitis may occur through modifying gut microbiota. To investigate the relationships of IN, colitis, and gut microbiomes, a dextran sulfate sodium (DSS)-induced mice colitis model was tested to explore the protective effects of IN on macroscopic colitis symptoms, the histopathological structure, inflammation cytokines, and gut microbiota, and their potential functions. Sulfasalazine (SASP) was used as the positive control. Firstly, because it was a mixture, the main chemical compositions of indigo and indirubin in IN were detected by ultra-performance liquid chromatography (UPLC). The clinical activity score (CAS), hematoxylin and eosin (H&E) staining results, and enzyme-linked immunosorbent assay (ELISA) results in this study showed that IN greatly improved the health conditions of the tested colitis mice, ameliorated the histopathological structure of the colon tissue, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines. The results of 16S rDNA sequences analysis with the Illumina MiSeq platform showed that IN could modulate the balance of gut microbiota, especially by down-regulating the relative quantity of Turicibacter and up-regulating the relative quantity of Peptococcus. The therapeutic effect of IN may be closely related to the anaerobic gram-positive bacteria of Turicibacter and Peptococcus. The inferred metagenomes from 16S data using PICRUSt demonstrated that decreased metabolic genes, such as through biosynthesis of siderophore group nonribosomal peptides, non-homologous end-joining, and glycosphingolipid biosynthesis of lacto and neolacto series, may maintain microbiota homeostasis during inflammation from IN treatment in DSS-induced colitis.

14.
BMC Cancer ; 19(1): 1111, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727020

RESUMO

BACKGROUND: To investigate whether MRI findings, including texture analysis, can differentiate KRAS mutation status in rectal cancer. METHODS: Totally, 158 patients with pathologically proved rectal cancers and preoperative pelvic MRI examinations were enrolled. Patients were stratified into two groups: KRAS wild-type group (KRASwt group) and KRAS mutation group (KRASmt group) according to genomic DNA extraction analysis. MRI findings of rectal cancers (including texture features) and relevant clinical characteristics were statistically evaluated to identify the differences between the two groups. The independent samples t test or Mann-Whitney U test were used for continuous variables. The differences of the remaining categorical polytomous variables were analyzed using the Chi-square test or Fisher exact test. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the discriminatory power of MRI features. The area under the ROC curve (AUC) and the optimal cut-off values were calculated using histopathology diagnosis as a reference; meanwhile, sensitivity and specificity were determined. RESULTS: Mean values of six texture parameters (Mean, Variance, Skewness, Entropy, gray-level nonuniformity, run-length nonuniformity) were significantly higher in KRASmt group compared to KRASwt group (p < 0.0001, respectively). The AUC values of texture features ranged from 0.703~0.813. In addition, higher T stage and lower ADC values were observed in the KRASmt group compared to KRASwt group (t = 7.086, p = 0.029; t = - 2.708, p = 0.008). CONCLUSION: The MRI findings of rectal cancer, especially texture features, showed an encouraging value for identifying KRAS status.

15.
BMJ Open ; 9(11): e034225, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727670

RESUMO

OBJECTIVES: Patient experience is being widely considered in the evaluation of healthcare service quality, which is a key target for public hospitals under China's New Healthcare Reform. This study aimed to illustrate patients' experiences in county-level public hospitals, and identify aspects that need to be improved. SETTING AND PARTICIPANTS: Between 2016 and 2018, a cross-sectional study with 500 outpatients and 800 inpatients was conducted in 10 county-level public hospitals from Shandong Province, Hubei Province and Chongqing Municipality. METHOD: A three-part questionnaire was used to evaluate patients' experiences during their visits to hospitals. It comprised a questionnaire for basic information, the Picker Patient Experience (PPE-15) Questionnaire and the overall evaluation (a 3-point Likert scale to express patients' satisfaction and patient loyalty). Patients' experiences were classified according to six dimensions (information transmission and patient education, respect for patient preference, emotional support, physical comfort, involvement of family or friends and continuity of medical service). Both univariate and multivariate analyses were performed to evaluate patient experience. RESULTS: A total of 1241 valid questionnaires were analysed. The mean PPE-15 score was 41.33 (range, 23-56). The better the patient experience and satisfaction, the higher the patient loyalty (p<0.001). Except for hospital disparities, patients' age and occupation status had a significant impact on patient experience (p<0.05). Of the six dimensions, the physical comfort score was the highest, while the respect for patient preference score was the lowest. Additionally, a strong correlation was found between the respect for patient preference dimension and patients' overall satisfaction with their treatment experience. CONCLUSIONS: Hospital managers and staff members should pay close attention to the preferences of patients and their families to improve patient experience.

16.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(5): 635-642, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31762230

RESUMO

OBJECTIVE: To investigate the mitochondrial translocation of hypoxia inducible factor-3α (HIF-3α) under normoxia and hypoxia and its physiological and pathological meanings. METHODS: ① After hypoxic (1%O2) or DMOG, CoCl2 treatments mimicking the hypoxic treatment, Western blot and immunofluorescence were used to examine the HIF-3α expression in mitochondria of HeLa and ACHN cells, respectively. ②The protease sensitivity experiment was used to explore the sub-organelle localization of HIF-3α in mitochondria. ③Western blot was used to examine mitochondrial HIF-3α in the normal mouse tissues and human liver carcinoma tissues. RESULTS: ① In HeLa and ACHN cells, HIF-3α translocated to mitochondria under normoxia and hypoxia, and its mitochondrial expression was higher under hypoxia; ②The protease sensitivity of HIF-3α was similar to proteins locating in the mitochondrial outer membrane; ③Mitochondrial HIF-3α expressed in multiple normal mouse tissues; The expression of mitochondrial HIF-3α was higher in human liver carcinoma tissues than the normal and adjacent tissues. CONCLUSIONS: HIF-3α translocated to mitochondrial outer membrane under both normoxia and hypoxia, and hypoxia could up-regulated HIF-3α mitochondrial translocation. Meanwhile, the phenomenon may be involved in the process of liver carcinoma.

17.
Int J Biol Macromol ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31739063

RESUMO

The present study aimed to evaluate effect of Ganoderma atrum polysaccharide (PSG) on acute lung injury (ALI) rats and its mechanisms. Results showed that PSG exhibited protective effects against ALI by maintaining pulmonary histology, reducing levels of pro-inflammatory cytokines and NO both in serum and lung tissue. Moreover, this study further evaluated the metabolic effects of PSG based on UPLC-Triple-TOF/MS metabolomics analysis in rats. Compared with control group, LysoPC (18:2), LPA (18:1), taurocholic acid, L-histidine, and L-tryptophan were identified as metabolic biomarkers in serum of ALI group. Furthermore, biological pathways analysis demonstrated that histidine metabolism, nitrogen metabolism, tryptophan and part glycerophospholipids metabolism were notably modified by PSG treatment in ALI rats. Additionally, improved gut microbial metabolite short-chain fatty acids were found after intake of PSG in ALI rat. Altogether, PSG could control ALI-induced aberrant inflammation and its mechanisms were linked to inhibit release of pro-inflammatory mediators and reverse metabolic pathway disturbances.

18.
Mar Pollut Bull ; : 110724, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31759635

RESUMO

The microplastics removal and its effects on membrane fouling in membrane bioreactor (MBR) for treating polluted surface water in drinking purpose was investigated in this study. Typical microplastics polyvinylchloride (PVC) with concentration 10 particles/L was added in the feed water. MBR was effective in treating organic matters and ammonia with removal rate over 80% and 95%, respectively. The removal performance was immediately inhibited with the microplastics PVC added into the MBR system, and recovered after operated for few days. The membrane fouling and cleaning results indicated that microplastics contamination could led to higher membrane fouling, and also the irreversible membrane fouling. The main contributor of rejection is the membrane module and the adsorption onto bio-carrier. The microbial community of the system before and after PVC addition did not show obvious difference. MBR has the potential to be used as effective technology in treating microplastics contaminated polluted surface water.

19.
ACS Nano ; 13(11): 13513-13523, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31714743

RESUMO

Red phosphorus (P) has been recognized as a promising material for lithium/sodium-ion batteries (LIBs/SIBs) because of their high theoretical capacity. However, tremendous volume variation and low conductivity limit its widespread applications. Hence, we design and synthesize uniformly distributed honeycomb-like hierarchical micro-mesoporous carbon nanospheres (HHPCNSs) with ultralarge pore volume (3.258 cm3 g-1) on a large scale through a facile way. The large pore volume provides enough space for loading of P and the expansion of P, and the uniform distribution of the micro-mesopores enables the red P to load uniformly. The resulting HHPCNSs/P composite exhibits extremely high capacity (2463.8 and 2367.6 mA h g-1 at 0.1 A g-1 for LIBs and SIBs, respectively), splendid rate performance (842.2 and 831.1 mA h g-1 at 10 A g-1 for LIBs and SIBs, respectively) and superior cycling stability (1201.6 and 938.4 mA h g-1 at 2 and 5 A g-1 after 1000 cycles for LIBs and 1269.4 and 861.8 mA h g-1 at 2 and 5 A g-1 after 1000 cycles for SIBs, respectively). More importantly, when coupled with LiFePO4 and Na3V2(PO4)3 cathode, lithium/sodium-ion full batteries display high capacity and superior rate and cycling performances, revealing the practicability of the HHPCNSs/P composite. The exceptional electrochemical performance is caused by the honeycomb-like carbon network with ultralarge pore volume, uniformly distributed hierarchical micro-mesoporous nanostructure, outstanding electronic conductivity, and excellent nanostructural stability, which is much better than currently reported P/C materials for both LIBs and SIBs.

20.
Brief Bioinform ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31774912

RESUMO

The methodologies for evaluating similarities between gene expression profiles of different perturbagens are the key to understanding mechanisms of actions (MoAs) of unknown compounds and finding new indications for existing drugs. L1000-based next-generation Connectivity Map (CMap) data is more than a thousand-fold scale-up of the CMap pilot dataset. Although several systematic evaluations have been performed individually to assess the accuracy of the methodologies for the CMap pilot study, the performance of these methodologies needs to be re-evaluated for the L1000 data. Here, using the drug-drug similarities from the Drug Repurposing Hub database as a benchmark standard, we evaluated six popular published methods for the prediction performance of drug-drug relationships based on the partial area under the receiver operating characteristic (ROC) curve at false positive rates of 0.001, 0.005 and 0.01 (AUC0.001, AUC0.005 and AUC0.01). The similarity evaluating algorithm called ZhangScore was generally superior to other methods and exhibited the highest accuracy at the gene signature sizes ranging from 10 to 200. Further, we tested these methods with an experimentally derived gene signature related to estrogen in breast cancer cells, and the results confirmed that ZhangScore was more accurate than other methods. Moreover, based on scoring results of ZhangScore for the gene signature of TOP2A knockdown, in addition to well-known TOP2A inhibitors, we identified a number of potential inhibitors and at least two of them were the subject of previous investigation. Our studies provide potential guidelines for researchers to choose the suitable connectivity method. The six connectivity methods used in this report have been implemented in R package (https://github.com/Jasonlinchina/RCSM).

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