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1.
Ecotoxicol Environ Saf ; 222: 112479, 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34224968

RESUMO

Lead is a widely distributed priority controlled heavy metals in aquatic system, its toxicity to aquatic organisms affected by water quality parameters. This study investigated the acute toxicity of lead (Pb) to the aquatic organisms in Taihu Lake under various water hardness, corresponding regional water quality criteria were derived. The acute toxicity experimental results revealed that the toxicity of Pb to aquatic organisms increased with water hardness. The Pb toxicity has a highest toxicity at water hardness 50 mg/L (expressed as CaCO3), especially for Palaemon modestus where the 96 h LC50 value was 0.024 mg/L. The hazardous concentration for 5% of biological species (HC5) values were determined via species sensitivity distribution (SSD) method as 94.0 µg/L, 222.3 µg/L and 375.8 µg/L for Pb at water hardness 50, 150, and 250 mg/L, respectively. The assessment factor (AF) value was set at 2, followed by the current SSD framework where European commission recommend a fixed AF of 5-1. Thus, the predicted no effect concentration (PNEC) values was 47.0 µg/L, 111.2 µg/Land 187.9 µg/L at water hardness 50, 150, and 250 mg/L, respectively. Meanwhile, the short-term water quality criteria of Pb for Taihu lake aquatic organisms were derived as 111.2 µg/L at water hardness 150 mg CaCO3/L. The long-term water quality criteria were derived as 4.3 µg/L by using acute/chronic ratio 51.29. When the derived value was used for Taihu Lake, 2.7% of the sampling sites in Taihu Lake was exceeded this criterion. The results of this study can provide technical methods and basic information for deriving Pb regional water quality criteria for protecting native aquatic organisms, in China.

2.
BMC Infect Dis ; 21(1): 647, 2021 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-34225644

RESUMO

BACKGROUND: Males and females differ in their immunological responses to foreign pathogens. However, most of the current COVID-19 clinical practices and trials do not take the sex factor into consideration. METHODS: We performed a sex-based comparative analysis for the clinical outcomes, peripheral immune cells, and severe acute respiratory syndrome coronavirus (SARS-CoV-2) specific antibody levels of 1558 males and 1499 females COVID-19 patients from a single center. The lymphocyte subgroups were measured by Flow cytometry. The total antibody, Spike protein (S)-, receptor binding domain (RBD)-, and nucleoprotein (N)- specific IgM and IgG levels were measured by chemiluminescence. RESULTS: We found that male patients had approximately two-fold rates of ICU admission (4.7% vs. 2.7% in males and females, respectively, P = 0.005) and mortality (3% vs. 1.4%, in males and females, respectively, P = 0.004) than female patients. Survival analysis revealed that the male sex is an independent risk factor for death from COVID-19 (adjusted hazard ratio [HR] = 2.22, 95% confidence interval [CI]: 1.3-3.6, P = 0.003). The level of inflammatory cytokines in peripheral blood was higher in males during hospitalization. The renal (102/1588 [6.5%] vs. 63/1499 [4.2%], in males and females, respectively, P = 0.002) and hepatic abnormality (650/1588 [40.9%] vs. 475/1499 [31.7%], P = 0.003) were more common in male patients than in female patients. By analyzing dynamic changes of lymphocyte subsets after symptom onset, we found that the percentage of CD19+ B cells and CD4+ T cells was generally higher in female patients during the disease course of COVID-19. Notably, the protective RBD-specific IgG against SARS-CoV-2 sharply increased and reached a peak in the fourth week after symptom onset in female patients, while gradually increased and reached a peak in the seventh week after symptom onset in male patients. CONCLUSIONS: Males had an unfavorable prognosis, higher inflammation, a lower percentage of lymphocytes, and indolent antibody responses during SARS-CoV-2 infection and recovery. Early medical intervention and close monitoring are important, especially for male COVID-19 patients.


Assuntos
Anticorpos Antivirais/sangue , COVID-19/imunologia , SARS-CoV-2/imunologia , Adulto , Idoso , Formação de Anticorpos , Feminino , Humanos , Imunoglobulina G/sangue , Subpopulações de Linfócitos/imunologia , Masculino , Pessoa de Meia-Idade , Caracteres Sexuais
3.
Sci Total Environ ; 794: 148710, 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-34214803

RESUMO

This study investigated the potential of improving methane production from algal sludge anaerobic digestion by peroxydisulfate (PDS) pretreatment. The results show that with PDS dosage at 0.02 g PDS/g algal sludge TSS, PDS added system has highest accumulative methane production after 60 days fermentation. The accumulative methane production was 1.08, 1.15, 1.14, 1.13, 1.08, 0.76, and 0.15 times as compared with control, at 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, and 1 g PDS/g algal sludge TSS added, respectively. The SCOD in the system was keep increasing with the increment of PDS dosage after 120 min pretreatment. The algal sludge dewatering rate was increased with adding of PDS as pretreatment. The addition of PDS has inhibited the activities of microbes involved in digestion, while the short chain fatty acids production was improved after 3 days digestion. One-substrate model can be used to simulate the methane yield. The hydrolysis rate was decreased after dosing with PDS, while highest actual and predicted accumulative methane yield was occurred at 0.02 g PDS/g algal sludge TSS. Proteobacteria has higher percentage when the PDS was not higher than 0.1 g PDS/g algal sludge TSS, Acetothermia has higher percentage at 0.01 g PDS/g algal sludge TSS. The microcystin-LR (MC-LR) in algal sludge was largely removed after digestion, including the intracellular MC-LR. The higher PDS dosage could cause heavy metal release from algae cell to the digestate during fermentation. The addition of PDS to algal sludge can improve the accumulative methane production and mitigate microcystin concentration.

4.
Int Microbiol ; 2021 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-34232406

RESUMO

INTRODUCTION: Serratia marcescens has attracted increasing attention worldwide as a neglected opportunistic pathogen of public health concern, especially due to its antimicrobial resistance features, which usually cause nosocomial infections in immunocompromised or critically ill patients. METHODS: In our study, four carbapenem-resistant Serratia marcescens (CRSM) clinical isolates were characterized in our hospital from February 2018 to May 2018. The conjugation experiment confirmed the transferability of the carbapenem resistance gene. The types of carbapenem resistance genes were detected by PCR. The homology of the strains was analysed by pulsed field gel electrophoresis (PFGE). The characteristics of the plasmid and environment of carbapenem resistance genes were analysed after whole genome sequencing was performed. Then, we compared the amino acid sequence of the replication initiation protein and constructed a dendrogram by the neighbour-joining method. RESULTS: All four isolates showed carbapenem resistance conferred by a blaKPC-2-harbouring plasmid. They had exactly the same bands confirmed by PFGE and were defined as the homologous strains. The blaKPC-2 genes in all of the isolates were located in a 42,742 bp plasmid, which was located in the core region of antibiotic resistance and was composed of Tn3 family transposons, recombinant enzyme genes, ISKpn6 and ISKpn27. The core region of antibiotic resistance formed a 'Tn3-ISKpn6-blaKPC-ISKpn27-Tn3' structure, which was an independent region as a movable element belonging to transposon Tn6296 and its derivatives. The plasmid had a similar skeleton to incX6 plasmids and a similar amino acid sequence to the replication initiation protein. The plasmid was defined as an untypeable blaKPC-2-harbouring plasmid named the 'IncX6-like' plasmid. CONCLUSION: The four CRSM isolates were mainly clonally disseminated with a blaKPC-2-harbouring plasmid in our hospital. The pKPC-2-HENAN1602 plasmid (CP047392) in our study was first reported in Serratia marcescens, which belongs to an untypeable group named the 'IncX6-like' plasmid. The carbapenem-resistant gene structure surrounding blaKPC-2 as a sole accessory module can be acquired by horizontal gene transfer and might lead to serious nosocomial infection.

5.
Reprod Sci ; 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34255312

RESUMO

Migration and invasion of trophoblasts is critical for human placental development, trophoblastic differentiation, and pregnancy-associated diseases. AT-rich interactive domain-containing protein 1A (ARID1A), a subunit of the SWI-SNF complex, has been suggested to participate in the regulation of fertility via placental disruption in mice. However, whether ARID1A regulates human placental development and function remains unknown. Here, using human trophoblast-like JEG-3 cell line, we report that ARID1A controls trophoblast cell migration and invasion. Overexpression of ARID1A inhibits JEG-3 cell migration and invasion, whereas knockdown of ARID1A promotes migration and invasion in JEG-3 cells. Mechanistically, while ARID1A reduces JEG-3 cell migration by down-regulation of Snail transcription, it restrains JEG-3 cell invasion by binding to and destabilization of MMP-9 protein. Finally, ARID1A is apparently up-regulated in placental tissues of preeclampsia compared to that of normal pregnancies. Our results thereby imply that ARID1A acts as a critical gene in supporting the physiological function of human mature placenta.

6.
Eur J Neurol ; 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34255403

RESUMO

OBJECTIVE: To provide an overview of genotype and phenotype distribution in a cohort of patients with Charcot-Marie-Tooth disease (CMT) and related disorders from Central South China. METHODS: We enrolled 435 patients and collected detailed clinical data. Multiplex ligation-dependent probe amplification for PMP22 duplication/deletion and CMT multi-gene panels sequencing were performed. Whole-exome sequencing was further applied in the remaining patients who failed to achieve molecular diagnosis. RESULTS: Among the 435 patients, 216 had CMT1, 14 had HNPP, 178 had CMT2, 24 had dHMN and 3 had HSAN. The overall molecular diagnosis rate was 70%: 75.7% in CMT1, 100% in HNPP, 64.6% in CMT2, 41.7% in dHMN, and 33.3% in HSAN. The most common four genotypes accounted for 68.9% of molecular diagnosed patients. Relatively frequent causes were missense changes in PMP22 (4.6%) and SH3TC2 (2.3%) in CMT1; and GDAP1 (5.1%), IGHMBP2 (4.5%) and MORC2 (3.9%) in CMT2. Twenty out of 160 detected pathogenic variants and the associated phenotypes were not previously reported. Broad phenotype spectra were observed in six genes, among which the pathogenic variants in BAG3 and SPTLC1 were detected in two sporadic patients presenting with CMT2 phenotype. CONCLUSIONS: Our results provided a unique genotypic and phenotypic landscape of patients with CMT and related disorders from Central South China, including relatively high proportion of CMT2 and lower occurrence of PMP22 duplication. The broad phenotype spectra in certain genes advanced our understanding of CMT.

8.
BMC Psychiatry ; 21(1): 330, 2021 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-34217234

RESUMO

BACKGROUND: The outbreak of Coronavirus Disease 2019(COVID-19) caused psychological stress in Chinese adults population. But we are unaware of whether the pandemic causes psychological stress on children. METHODS: We used the Children's Impact of Event Scale questionnaire (CRIES-13) to investigate the degree of Post-traumatic Stress (PTSD) symptoms caused by the pandemic in students selected from schools in Sichuan, Jiangsu, Henan, Yunnan, and Chongqing provinces of China. RESULTS: A total of 7769 students(3692 male and 4077 female), aged 8-18 years, were enrolled in the study, comprising 1214 in primary schools, 2799 in junior high schools and 3756 in senior high schools. A total of 1639 students (21.1%) had severe psychological stress reactions. A large proportion of senior high school students (23.3%) experienced severe psychological stress, and they had the highest median total CRIES-13 score. Female students were more likely to experience severe psychological stress and had higher median CRIES-13 total scores than males. CONCLUSION: COVID-19 has placed psychological stresses on primary and secondary school students in China. These stresses are more likely to reach severe levels among female students and senior high school students.


Assuntos
COVID-19 , Transtornos de Estresse Pós-Traumáticos , Adulto , Criança , China/epidemiologia , Depressão , Surtos de Doenças , Feminino , Humanos , Masculino , SARS-CoV-2 , Instituições Acadêmicas , Transtornos de Estresse Pós-Traumáticos/epidemiologia , Estudantes , Inquéritos e Questionários
9.
Virus Res ; : 198506, 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34271040

RESUMO

Carbapenem-resistant Klebsiella pneumoniae (CRKP) have spread globally and led to the limited choice of antimicrobial treatment of K. pneumoniae-induced infections. Bacteriophages are considered as an effective strategy against bacterial infections. In this study, we isolated a novel Klebsiella phage BUCT556A with lytic activity against KPC-producing K. pneumoniae, which was a multi-drug resistant isolate. Phage BUCT556A had a symmetrical head and a long, non-contractile tail, belonging to the family Siphoviridae, order Caudoviridae. Phage BUCT556A had a relatively narrow host range, and a medium burst size of 91 PFU/cell. It was stable at broad temperature/pH range, and exhibited good tolerance to chloroform. The genome of phage BUCT556A was a 49, 376-bp linear double-stranded DNA molecule with average G+C content of 50.2%, and contained 75 open reading frames. There was no tRNA, antibiotic resistance, toxin, virulence related genes or lysogen-formation gene clusters detected in the genome of phage BUCT556A. Phylogenetic analyses based on the major capsid protein Mcp suggested that this phage had a close relationship with Klebsiella phage KLPN1. Together, through phenotypic combined with genomic DNA sequencing and analyses, our study suggests that phage BUCT556A has the potential to be used as a bacterial treatment tool for multidrug-resistant strains K. pneumoniae.

10.
J Zhejiang Univ Sci B ; 22(7): 599-602, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34269012

RESUMO

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has occasioned worldwide alarm. Globally, the number of reported confirmed cases has exceeded 84.3 million as of this writing (January 2, 2021). Since there are no targeted therapies for COVID-19, the current focus is the repurposing of drugs approved for other uses. In some clinical trials, antiviral drugs such as remdesivir (Grein et al., 2020), lopinavir/ritonavir (LPV/r) (Cao et al., 2020), chloroquine (Gao et al., 2020), hydroxychloroquine (Gautret et al., 2020), arbidol (Wang et al., 2020), and favipiravir (Cai et al., 2020b) have shown efficacy in COVID-19 patients. LPV/r combined with arbidol, which is the basic regimen in some regional hospitals in China including Zhejiiang Province, has shown antiviral effects in COVID-19 patients (Guo et al., 2020; Xu et al., 2020). A retrospective cohort study also reported that this combination therapy showed better efficacy than LPV/r alone for the treatment of COVID-19 patients (Deng et al., 2020).

11.
BMC Infect Dis ; 21(1): 647, 2021 Jul 05.
Artigo em Inglês | MEDLINE | ID: covidwho-1298047

RESUMO

BACKGROUND: Males and females differ in their immunological responses to foreign pathogens. However, most of the current COVID-19 clinical practices and trials do not take the sex factor into consideration. METHODS: We performed a sex-based comparative analysis for the clinical outcomes, peripheral immune cells, and severe acute respiratory syndrome coronavirus (SARS-CoV-2) specific antibody levels of 1558 males and 1499 females COVID-19 patients from a single center. The lymphocyte subgroups were measured by Flow cytometry. The total antibody, Spike protein (S)-, receptor binding domain (RBD)-, and nucleoprotein (N)- specific IgM and IgG levels were measured by chemiluminescence. RESULTS: We found that male patients had approximately two-fold rates of ICU admission (4.7% vs. 2.7% in males and females, respectively, P = 0.005) and mortality (3% vs. 1.4%, in males and females, respectively, P = 0.004) than female patients. Survival analysis revealed that the male sex is an independent risk factor for death from COVID-19 (adjusted hazard ratio [HR] = 2.22, 95% confidence interval [CI]: 1.3-3.6, P = 0.003). The level of inflammatory cytokines in peripheral blood was higher in males during hospitalization. The renal (102/1588 [6.5%] vs. 63/1499 [4.2%], in males and females, respectively, P = 0.002) and hepatic abnormality (650/1588 [40.9%] vs. 475/1499 [31.7%], P = 0.003) were more common in male patients than in female patients. By analyzing dynamic changes of lymphocyte subsets after symptom onset, we found that the percentage of CD19+ B cells and CD4+ T cells was generally higher in female patients during the disease course of COVID-19. Notably, the protective RBD-specific IgG against SARS-CoV-2 sharply increased and reached a peak in the fourth week after symptom onset in female patients, while gradually increased and reached a peak in the seventh week after symptom onset in male patients. CONCLUSIONS: Males had an unfavorable prognosis, higher inflammation, a lower percentage of lymphocytes, and indolent antibody responses during SARS-CoV-2 infection and recovery. Early medical intervention and close monitoring are important, especially for male COVID-19 patients.

12.
BMC Psychiatry ; 21(1): 330, 2021 07 03.
Artigo em Inglês | MEDLINE | ID: covidwho-1295451

RESUMO

BACKGROUND: The outbreak of Coronavirus Disease 2019(COVID-19) caused psychological stress in Chinese adults population. But we are unaware of whether the pandemic causes psychological stress on children. METHODS: We used the Children's Impact of Event Scale questionnaire (CRIES-13) to investigate the degree of Post-traumatic Stress (PTSD) symptoms caused by the pandemic in students selected from schools in Sichuan, Jiangsu, Henan, Yunnan, and Chongqing provinces of China. RESULTS: A total of 7769 students(3692 male and 4077 female), aged 8-18 years, were enrolled in the study, comprising 1214 in primary schools, 2799 in junior high schools and 3756 in senior high schools. A total of 1639 students (21.1%) had severe psychological stress reactions. A large proportion of senior high school students (23.3%) experienced severe psychological stress, and they had the highest median total CRIES-13 score. Female students were more likely to experience severe psychological stress and had higher median CRIES-13 total scores than males. CONCLUSION: COVID-19 has placed psychological stresses on primary and secondary school students in China. These stresses are more likely to reach severe levels among female students and senior high school students.


Assuntos
COVID-19 , Transtornos de Estresse Pós-Traumáticos , Adulto , Criança , China/epidemiologia , Depressão , Surtos de Doenças , Feminino , Humanos , Masculino , SARS-CoV-2 , Instituições Acadêmicas , Transtornos de Estresse Pós-Traumáticos/epidemiologia , Estudantes , Inquéritos e Questionários
13.
Psychol Health Med ; : 1-9, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: covidwho-1291741

RESUMO

This study aimed to investigate the prevalence of mental problems and social support among nurses during the COVID-19 epidemic and to explore the correlation. We carried out a multicentre, large-sample questionnaire survey in Chongqing (China). The WeChat-based survey program Questionnaire Star was used to distribute a questionnaire with self-designed items to obtain general information, the Depression, Anxiety and Stress Scale-21 (DASS-21), and the Social Support Rating Scale (SSRS). A total of 848 neonatal nurses participated. The results showed that 104 nurses (12.3%) had depression symptoms, 133 (15.7%) had anxiety symptoms, and 45 (5.3%) had stress symptoms. However, the DASS-21 score of the majority of nurses was normal. Pearson correlation analyses revealed that mental health was negatively correlated with social support, indicating that the higher social support was, the better the psychological condition of nurses.

14.
Front Immunol ; 12: 614773, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34276642

RESUMO

Human leukocyte antigen G (HLA-G) is known as a novel immune checkpoint molecule in cancer; thus, HLA-G and its receptors might be targets for immune checkpoint blockade in cancer immunotherapy. The aim of this study was to systematically identify the roles of checkpoint HLA-G molecules across various types of cancer. ONCOMINE, GEPIA, CCLE, TRRUST, HAP, PrognoScan, Kaplan-Meier Plotter, cBioPortal, LinkedOmics, STRING, GeneMANIA, DAVID, TIMER, and CIBERSORT were utilized. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed. In this study, we comprehensively analysed the heterogeneous expression of HLA-G molecules in various types of cancer and focused on genetic alterations, coexpression patterns, gene interaction networks, HLA-G interactors, and the relationships between HLA-G and pathological stage, prognosis, and tumor-infiltrating immune cells. We first identified that the mRNA expression levels of HLA-G were significantly upregulated in both most tumor tissues and tumor cell lines on the basis of in-depth analysis of RNAseq data. The expression levels of HLA-G were positively associated with those of the other immune checkpoints PD-1 and CTLA-4. Abnormal expression of HLA-G was significantly correlated with the pathological stage of some but not all tumor types. There was a significant difference between the high and low HLA-G expression groups in terms of overall survival (OS) or disease-free survival (DFS). The results showed that HLA-G highly expressed have positive associations with tumor-infiltrating immune cells in the microenvironment in most types of tumors (P<0.05). Additionally, we identified the key transcription factor (TF) targets in the regulation of HLA-G expression, including HIVEP2, MYCN, CIITA, MYC, and IRF1. Multiple mutations (missense, truncating, etc.) and the methylation status of the HLA-G gene may explain the differential expression of HLA-G across different tumors. Functional enrichment analysis showed that HLA-G was primarily related to T cell activation, T cell regulation, and lymphocyte-mediated immunity. The data may provide novel insights for blockade of the HLA-G/ILT axis, which holds potential for the development of more effective antitumour treatments.

15.
J Environ Manage ; 296: 113175, 2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34243093

RESUMO

Zinc is a widely distributed environmental pollutants and has been listed as priority heavy metal pollutant in China. Similar as other heavy metals, toxicity of zinc to aquatic organisms affects by environmental factors such as water hardness. It is necessary to develop regional water quality criteria (WQC) to protect native aquatic life against zinc due to the diversity of aquatic organisms' variability across different water systems, as a concretization and supplement for national zinc WQC. This study derived WQC for zinc by species sensitivity distribution (SSD) curve method. The zinc toxicity data of the aquatic organisms in Taihu Lake used in SSD curve was collected based on published toxicity data for zinc with hardness values and supplemented with acute toxicity tests conducted in this study. Six aquatic organism natives to Taihu Lake were selected to conduct zinc acute toxicity test in a range of hardness conditions. The relationship between water hardness and zinc toxicity was constructed. The criterion maximum concentration (CMC) and criterion continuous concentration (CCC) for zinc in Taihu Lake were then derived, which considered the water quality and taxonomic groups in Taihu Lake. The CMC and CCC were 100.69 µg/L and 30.79 µg/L, respectively. The environmental risk of zinc to Taihu Lake are acceptable, at moderate to low levels. This study has provided a basis for regional water quality criterion derivation and risk assessment in China.

16.
Nat Commun ; 12(1): 4161, 2021 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-34230488

RESUMO

Given the pleiotropic nature of coding sequences and that many loci exhibit multiple disease associations, it is within non-coding sequence that disease-specificity likely exists. Here, we focus on joint disorders, finding among replicated loci, that GDF5 exhibits over twenty distinct associations, and we identify causal variants for two of its strongest associations, hip dysplasia and knee osteoarthritis. By mapping regulatory regions in joint chondrocytes, we pinpoint two variants (rs4911178; rs6060369), on the same risk haplotype, which reside in anatomical site-specific enhancers. We show that both variants have clinical relevance, impacting disease by altering morphology. By modeling each variant in humanized mice, we observe joint-specific response, correlating with GDF5 expression. Thus, we uncouple separate regulatory variants on a common risk haplotype that cause joint-specific disease. By broadening our perspective, we finally find that patterns of modularity at GDF5 are also found at over three-quarters of loci with multiple GWAS disease associations.

17.
Infect Genet Evol ; : 104983, 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-34197916

RESUMO

INTRODUCTION: Previous studies have indicated that IL33/ST2 pathway is strongly involved in HBV-related liver diseases. The aim of this study was to determine the relationship of genetic variants in IL33/ST2 pathway with susceptibility of liver cirrhosis. MATERIALS AND METHODS: The study subjects included 2632 samples of Han Chinese population, including 840 negative controls (NeC), 691 chronic hepatitis B (CHB), 680 HBV-related liver cirrhosis (LC), and 421 HBV-related HCC patients. DNA was extracted from peripheral blood. Genotyping of IL33-rs4742170, rs1048274, rs10975519 and IL1RL1-rs1041973 was performed using MALDI-TOF-MS. RESULTS: After adjustment for age, sex, smoking and drinking, significant associations were observed for IL33-rs4742170, rs1048274, rs10975519 polymorphisms with LC risk. However, there was no association between IL1RL1-rs1041973 and LC risk. Negative controls with IL33-rs4742170 CC genotype showed 1.800 times more likely to develop LC compared with TT genotype. Negative controls with rs10975519(TC+CC) genotype showed 1.318 times more likely to develop LC compared with TT genotype. CHB cases with rs4742170(CC+TC) genotype showed 1.302 times higher susceptibility to develop LC compared with TT genotype. The IL33-rs1048274G allele occurred more frequently in LC group than HCC group in codominant model (AG/AA: P = 0.001, OR = 1.656, 95%CI = 1.221-2.247; GG/AA: P = 0.018, OR = 1.539, 95%CI = 1.077-2.198). The IL33 haplotype CG conformed by rs10975519C and rs1048274G was more frequent in LC group than NeC group and CHB group. Moreover, the IL33 haplotype CCG conformed by rs4742170C, rs10975519C and rs1048274G was more frequent in LC group than HCC group. CONCLUSION: Our findings demonstrate the association of genetic variants in IL33 with susceptibility to liver cirrhosis. IL33-rs4742170C, rs1048274G, rs10975519C may serve as biomarkers for the risk of LC.

18.
Psychol Health Med ; : 1-9, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34192997

RESUMO

This study aimed to investigate the prevalence of mental problems and social support among nurses during the COVID-19 epidemic and to explore the correlation. We carried out a multicentre, large-sample questionnaire survey in Chongqing (China). The WeChat-based survey program Questionnaire Star was used to distribute a questionnaire with self-designed items to obtain general information, the Depression, Anxiety and Stress Scale-21 (DASS-21), and the Social Support Rating Scale (SSRS). A total of 848 neonatal nurses participated. The results showed that 104 nurses (12.3%) had depression symptoms, 133 (15.7%) had anxiety symptoms, and 45 (5.3%) had stress symptoms. However, the DASS-21 score of the majority of nurses was normal. Pearson correlation analyses revealed that mental health was negatively correlated with social support, indicating that the higher social support was, the better the psychological condition of nurses.

19.
Biol Pharm Bull ; 44(7): 902-909, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34193687

RESUMO

Local anesthetic toxicity is closely related to neuronal death and activation of the inflammatory response. Dexmedetomidine (Dex) is an adrenergic α2 receptor agonist that can reduce the neurotoxicity induced by lidocaine. It also has anti-inflammatory effects. However, the mechanism underlying the neuroprotective effects of Dex against lidocaine-induced toxicity remains to be defined. We hypothesized that Dex exerts its neural protective effect through inhibiting inflammasome activation and through anti-pyroptosis effects against local anesthetic-induced nerve injury. In a rat model of lidocaine-induced spinal cord injury, we studied the protective effect of Dex on lidocaine-induced changes in spinal cord function, inflammasome formation and pyroptosis, pro-inflammatory cytokine expression, and protein kinase C (PKC)-δ phosphorylation. Dex reduced lidocaine-induced neurotoxicity and inhibited PKC-δ phosphorylation in the spinal cord of rats. Furthermore, Dex inhibited pyroptosis and inflammasome formation (caspase-1, NLRP3, and apoptosis-associated speck-like protein (ASC)). Finally, Dex attenuated interleukin (IL)-1ß and IL-18 expression, as well as microglia response. In conclusion, Dex can reduce the severity of lidocaine-induced spinal cord injury in rats by inhibiting priming and inflammasome activation and reducing pyroptosis via PKC-δ phosphorylation.

20.
Adv Rheumatol ; 61(1): 36, 2021 06 12.
Artigo em Inglês | MEDLINE | ID: mdl-34118994

RESUMO

OBJECTIVES: To investigate the diagnostic performance of single-source dual-energy computed tomography (DECT) based on gemstone spectral imaging technology (including Discovery CT750HD and Revolution CT) in patients with suspected feet/ankles gouty arthritis, and evaluate the urate deposition with a novel semi-quantitative DECT scoring system. METHODS: A total of 196 patients were consecutively included. Feet and ankles were evaluated in all patients by single-source DECT scan. The 2015 EULAR/ACR criteria were used as the reference for the diagnosis of gout. The sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) of DECT for the diagnosis of gout in the early (≤1 year), middle (1-3 years), and late (> 3 years) disease durations were calculated. Besides, a novel semi-quantitative DECT scoring system was assessed for the measurement of urate deposition, and the correlation between the scores and the clinical and serological data were also evaluated. Moreover, the influences of artifacts on the diagnostic performance of DECT were also determined. RESULTS: The sensitivity, specificity, and AUC of DECT in 196 patients were 38.10, 96.43%, and 0.673 in the early-stage group; 62.96, 100.00%, and 0.815 in the middle-stage group; and 77.55, 87.50%, and 0.825 in the late-stage group, respectively. The overall diagnostic accuracies in the AUC of DECT (Discovery CT750HD and Revolution CT) in the middle and late stages of gout were higher than that in the early stage of gout. Besides, the monosodium urate crystals were deposited on the first metatarsophalangeal joints and ankles/midfeet. Age, the presence of tophus, bone erosion, and disease duration considerably affected the total urate score. No statistical difference in the positive detection of nail artifact, skin artifact, vascular calcification, and noise artifact was found between the case and control groups. CONCLUSION: DECT (Discovery CT750HD and Revolution CT) showed promising diagnostic accuracy for the detection of urate crystal deposition in gout but had limited diagnostic sensitivity for short-stage gout. Longer disease duration, the presence of tophus, and bone erosion were associated with the urate crystal score system. The artifacts do not remarkably affect the diagnostic performance of DECT in gout.

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