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1.
Oral Dis ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33031609

RESUMO

OBJECTIVE: Experiments were performed to evaluate CYLD expression in human gingival tissue samples and to examine the effects of CYLD on inflammatory responses in lipopolysaccharide (LPS)- or TNF-α-stimulated human gingival fibroblasts (HGFs). METHODS: Immunohistochemistry for CYLD and p65 expression was performed with healthy and inflamed gingival tissue samples. siRNA was used to knock down the expression of CYLD in HGFs. Upon LPS or TNF-α stimulation, NF-κB activation was detected in control and CYLD-knockdown HGFs. RT-PCR was applied to determine gene expression. Western blot analyses were employed to assess protein expression. Immunofluorescence staining was carried out to evaluate the nuclear translocation of p65. RESULTS: Immunohistochemical staining showed the expression of CYLD in human gingival tissues. In addition, CYLD protein expression was reduced in inflamed gingival tissue samples compared with healthy tissue samples. CYLD knockdown greatly enhanced the mRNA expression of proinflammatory cytokines in LPS- or TNF-α-stimulated HGFs. Furthermore, knocking down CYLD expression increased LPS-stimulated NF-κB activation in HGFs. Unexpectedly, CYLD knockdown did not affect TNF-α-induced NF-κB activation. CONCLUSIONS: Our results suggest that CYLD participates in periodontal inflammatory responses by negatively regulating LPS-induced NF-κB signalling.

2.
Vet Parasitol ; 286: 109254, 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-33032075

RESUMO

Neospora caninum (N. caninum) is an intracellular parasite and is the causative agent of neosporosis, which leads to reproductive failure in cattle. Pyroptosis is a recently discovered form of programmed cell death executed by gasdermin D (GSDMD). This cell death mechanism is an important host defense against intracellular pathogens. However, pyroptosis induced by N. caninum is poorly understood. The aim of this study was to explore the roles of GSDMD-mediated pyroptosis during N. caninum infection in vivo. N. caninum-infected wild type mice and GSDMD-deficient mice were used to evaluate host resistance and its ability to affect immune response against this parasite. The results showed that GSDMD deficiency significantly reduced survival and impaired the host's abilities to clear parasite loads in tissues, monocytes/macrophages and neutrophils. Additionally, GSDMD was essential for circulating IL-18 and IFN-γ production induced by N. caninum infection, indicating that GSDMD can mediate the Th 1 immune response against N. caninum infection. Additional data revealed that treatment with exogenous recombinant IL-18 in N. caninum-infected Gsdmd-/- mice rescues the reduction of circulating IFN-γ production to help eliminate the parasite. Taken together, our data indicate that GSDMD-mediated pyroptosis plays a vital role in maintaining host resistance to N. caninum and is essential for clearing the parasite. This form of programmed cell death promotes the Th 1 immune response by controlling IL-18 release and is considered a host defense against N. caninum. This study expands our understanding of interactions between host immune response/defense and N. caninum infection.

3.
J Nanobiotechnology ; 18(1): 142, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33008457

RESUMO

This review focuses on the recent advances in the synthesis of graphene quantum dots (GQDs) and their applications in drug delivery. To give a brief understanding about the preparation of GQDs, recent advances in methods of GQDs synthesis are first presented. Afterwards, various drug delivery-release modes of GQDs-based drug delivery systems such as EPR-pH delivery-release mode, ligand-pH delivery-release mode, EPR-Photothermal delivery-Release mode, and Core/Shell-photothermal/magnetic thermal delivery-release mode are reviewed. Finally, the current challenges and the prospective application of GQDs in drug delivery are discussed.

4.
Int J Immunopathol Pharmacol ; 34: 2058738420963818, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33016797

RESUMO

Sepsis, a severe infectious disease in the neonatal period, is considered a risk factor for necrotizing enterocolitis (NEC). To investigate the specific risk factors for NEC in septic infants, septic infants admitted to our center from January 2010 to April 2018 were included. Septic neonates with proven NEC (Bell's stage ⩾II) were enrolled in the NEC group, and those without NEC were enrolled in the control group. Demographics, clinical characteristics, and risk factors were compared between the two groups. Univariate and logistic regression analyses were used to evaluate the potential risk factors for NEC. A total of 610 septic neonates were included, of whom 78 (12.8%) had complicated NEC. The univariate analysis indicated that infants with NEC had a lower birth weight, a lower gestational age, and older age on admission than those without NEC (P < 0.05). Higher rates of anemia, prolonged rupture of membranes (PROM) (⩾18 h), pregnancy-induced hypertension, late-onset sepsis (LOS), red blood cell transfusion and hypoalbuminemia were observed in the NEC group than in the non-NEC group (P<0.05). Logistic regression analysis revealed LOS (P = 0.000), red blood cell transfusion (P = 0.001) and hypoalbuminemia (P = 0.001) were associated with the development of NEC. Among NEC infants, those who needed red blood cell transfusion had a longer hospitalization duration than those who did not need transfusion (P < 0.05). LOS, red blood cell transfusion and hypoalbuminemia were independent risk factors for the development of NEC in infants with sepsis. Taking measures to reduce the occurrence of hypoproteinemia and severe anemia may help to reduce the occurrence of NEC in septic neonates.

5.
Artigo em Inglês | MEDLINE | ID: mdl-33023158

RESUMO

In rural China around 60 million left-behind children (LBC) experience prolonged separation from migrant worker parents. They are vulnerable to a range of psychosocial problems. The aim of this study was to determine whether a community-based intervention consisting of Children's Centres can improve psychosocial well-being and school performance of these children. The intervention was carried out in 20 villages, for children aged 7 to 15 years, irrespective of left-behind status. Nine hundred and twenty children, 438 LBC and 256 children living with parents (RC) attended the Centres. At follow-up after one year, there were improvements compared to baseline in total difficulties (measured with the Strengths and Difficulties Questionnaire) in children left behind by both parents (p = 0.009), children left behind by one parent (p = 0.008) and RC (p = 0.05). Postintervention school performance significantly improved in both categories of LBC (p < 0.001), but not RC (p = 0.07); social support score increased in both categories of LBC (p < 0.001) and RC (p = 0.01). Findings from interviews with key stakeholders were overwhelmingly positive about the impacts. With strong local leadership and community motivation, a low-cost intervention can improve children's psychosocial well-being in these settings. Allowing communities to adapt the model to their own situation fosters local ownership, commitment, with benefits for children, parents, carers, and communities.

6.
Nanotechnology ; 32(1): 015202, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33043902

RESUMO

The investigation of two-dimensional (2D) nonlinear optical materials offers a promising way to construct the high-performance optical devices in fundamental and industrial applications because of their rich distinct optoelectronic properties. Herein, by utilizing the liquid exfoliation method, vanadium disulfide (VS2) nanosheets are prepared and the thickness is measured to be 3.16 nm. In addition, we have fabricated the VS2-based optical device and the nonlinear optical property is characterized with modulation depth of 23.97%. By using VS2 as saturable absorber, a high stable passively mode-locking Er-doped fiber laser is obtained with pulse duration of 169 fs and the largest average output power of 70.5 mW. The slope efficiency is up to 7.9%. In comparison to recent results of mode-locking fiber lasers with 2D materials, the VS2-based fiber laser demonstrates better performance. To the best of our knowledge, this is the first example of using VS2 for generating femtosecond mode-locked laser pulse. Our experimental results not only reveal VS2 ultrafast photonics application, but also advance the high-performance applications for information science and nonlinear optics.

7.
BMC Ophthalmol ; 20(1): 402, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028272

RESUMO

BACKGROUND: Optical coherence tomography angiography (OCTA) is a newly developed imaging quantitative technique for analysis of choriocapillaris (CC) flow changes, thereby exploring the pathological mechanism of chronic central serous chorioretinopathy (CCSC) and the therapeutic effects of photodynamic therapy (PDT). In this study, we sought to quantify the blood flow changes in CC of CCSC patients receiving half-dose PDT using OCTA. METHODS: A total of 28 affected eyes and 24 unaffected eyes of 26 CCSC patients receiving half-dose PDT, and 40 eyes of 20 healthy gender- and age-matched subjects were retrospectively enrolled in this study. The proportion of total areas of flow signal voids (FSV, %) in CC level of OCTA was assessed in both eyes of the CCSC patients at baseline and repeated in multiple sections at 1-week, 1-month, 3-month and 6-month intervals after PDT. In addition, the CC patterns in response to PDT at early stage and the subsequent morphologic changes were qualitatively documented using OCTA. RESULTS: For affected eyes, FSV at 6-m follow-up was significantly lower than that at 1-m follow-up (p = 0.036). When compared to normal control eyes, FSV in affected eyes was significantly higher at 1-m, 3-m and 6-m follow-up (p < 0.05 for all), and FSV in unaffected eyes was significantly higher at baseline, 1-w, 1-m and 3-m follow-up (p < 0.05 for all). Three CC patterns of early response to PDT were identified, including signs of recovery with more even flow signals, transient appearance of worse ischemia and secondary neovascularization within CC level. CONCLUSION: Abnormal CC flow attenuation remains in completely resolved eyes of CCSC patients treated with half-dose PDT.

8.
Front Immunol ; 11: 564768, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33042143

RESUMO

Immune response in the liver is determined by the spatial organization and cellular dynamics of hepatic immune cells. The liver vasculature accommodates abundant tissue-resident innate immune cells, such as Kupffer cells, natural killer cells, and natural killer T cells, to ensure efficient intravascular immunosurveillance. The fenestrated sinusoids also allow direct contact between circulating T cells and non-canonical antigen-presenting cells, such as hepatocytes, to instruct adaptive immune responses. Distinct cellular behaviors are exploited by liver immune cells to exert proper functions. Intravital imaging enables real-time visualization of individual immune cell in living animals, representing a powerful tool in dissecting the spatiotemporal features of intrahepatic immune cells during steady state and liver diseases. This review summarizes current advances in liver immunology prompted by in vivo imaging, with a particular focus on liver-resident innate immune cells and hepatic T cells.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33001810

RESUMO

Stroke is an acute cerebral vascular disease that is likely to cause long-term disabilities and death. Immediate emergency care with accurate diagnosis of computed tomographic (CT) images is crucial for dealing with a hemorrhagic stroke. However, due to the high variability of a stroke's location, contrast, and shape, it is challenging and time-consuming even for experienced radiologists to locate them. In this paper, we propose a U-net based deep learning framework to automatically detect and segment hemorrhage strokes in CT brain images. The input of the network is built by concatenating the flipped image with the original CT slice which introduces symmetry constraints of the brain images into the proposed model. This enhances the contrast between hemorrhagic areas and normal brain tissue. Various Deep Learning topologies are compared by varying the layers, batch normalization, dilation rates, and pre-train models. This could increase the respective filed and preserves more information on lesion characteristics. Besides, the adversarial training is also adopted in the proposed network to improve the accuracy of the segmentation. The proposed model is trained and evaluated on two different datasets, which achieve the competitive performance with human experts with the highest location accuracy 0.9859 for detection, 0.8033 Dice score, and 0.6919 IoU for segmentation. The results demonstrate the effectiveness, robustness, and advantages of the proposed deep learning model in automatically hemorrhage lesion diagnosis, which make it possible to be a clinical decision support tool in stroke diagnosis.

10.
J Dent ; : 103490, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-33007363

RESUMO

OBJECTIVES: To evaluate scientific evidence about the impact of implant location on the prevalence of peri-implantitis at implant level. SOURCE: Databases (PubMed, Embase and Cochrane) were searched until March 2020without limitations on language or publication year. Hand searches and gray literature were also included. STUDY SELECTION: Clinical studies reporting data on prevalence of peri-implantitis in anterior and posterior regions at implant level, and evaluations of implants with at least 1 year of function were selected. DATA: Ten studies with a low risk of bias were included. Meta-analysis was performed to estimate the pooled risk ratio. A significantly higher prevalence of peri-implantitis in anterior region compared to posterior region was found (Risk ratio: 1.34; 95% CI: [1.07, 1.69]; p = 0.01). Meta-regression was performed to analyze the potential influence of confounding factors by calculating p-value of the coefficient. Subjects (p = 0.827), implants (p = 0.859) and age (p = 0.656) did not significantly influence the outcome. Subgroup analysis by jaw revealed significantly higher prevalence of peri-implantitis in maxillary anterior (Risk ratio: 1.37; 95% CI: [1.10, 1.71]; p = 0.005) and mandibular anterior (Risk ratio: 1.76; 95% CI: [1.29, 2.42]; p = 0.0004) regions compared to maxillary posterior region. No significant difference was found between maxillary anterior and mandibular posterior (Risk ratio: 1.15; 95% CI: [0.75, 1.75]; p = 0.53) regions. A meta-analysis was precluded between mandibular anterior and mandibular posterior regions due to high statistical heterogeneity (I 2 = 76%). CONCLUSIONS: Implants in the maxillary anterior and mandibular anterior regions had a higher prevalence of peri-implantitis compared to the maxillary posterior region. CLINICAL SIGNIFICANCE: Practitioners should strictly grasp the indications for patients missing anterior teeth and make comprehensive treatment planning.

11.
J Hazard Mater ; 404(Pt A): 124015, 2020 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-33039827

RESUMO

4-Hydroxyphenylpyruvatedioxygenase (HPPD) is a Fe(II)/Co(II)-dependent enzyme which has become one of the most effective herbicide targets. HPPD inhibitors have been developed as efficient herbicides for resistant weed control. Developing a method for efficient and rapid HPPD inhibitors detection is still challenging. N-n-butyl-4-methylhydrazinecarbothioamide-1,8-naphthalimide (NMN) was synthesized and used to detect Co2+ efficiently with the limit of detection (LOD) of 7.82 nM with a turn-on fluorescence. Herein a novel fluorescent complex, NMN‒Co2+ was employed to determine HPPD inhibitors which performed a turn-off effect in the sensing process based on the competitive coordination between the probe and HPPD with Co2+. The LODs for three commercial triketone HPPD inhibitors (mesotrione, tembotrione and NTBC) were 6.60 nM, 7.37 nM and 10.22 nM with good sensitivity and selectivity. Furthermore, the present probe has potentials to quantitatively detect mesotrione and tembotrione in real samples.

12.
Ann Hepatol ; 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33059056

RESUMO

INTRODUCTION AND OBJECTIVES: Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) may be closely associated with Hepatitis C virus (HCV) infection and the development of hepatocellular carcinoma (HCC). In this study, we investigated the expression and functions of a lncRNA, LINC01189, in HCV-associated HCC. PATIENTS OR MATERIALS AND METHODS: LINC01189 expression was measured in HCC tumors, HCV-infected HCC tumors and HCV-infected HCC cells. LINC01189 was overexpressed in HCV-infected HepG2 cells to measure its function on HCV-correlated cancer proliferation. In HCC cell lines of Huh7 and Hep3B, LINC01189 was upregulated to investigate its effects on cancer cell proliferation and 5-FU chemoresistance. The competing endogenous RNA (ceRNA) target of LINC01189, human microRNA-155-5p (hsa-miR-155-5p) was probed by dual-luciferase assay and qRT-PCR. Hsa-miR-155-5p was upregulated in LINC01189-overexpessed Huh7 and Hep3B cells to investigate their epigenetic correlation on HCC development regulation. RESULTS: LINC01189 is downregulated in HCV-infected HCC tumors and cell lines. LINC01189 overexpression inhibited HCC cancer cell proliferation and 5-FU chemoresistance. Hsa-miR-155-5p was confirmed to be a ceRNA target of LINC01189 in HCC. Upregulating hsa-miR-155-5p reversed the LINC01189-mediated inhibition on HCC proliferation and 5-FU chemoresistance. CONCLUSIONS: LINC01189 downregulation is associated with HCV infection in HCC, and it has tumor-suppressing effects on HCC development through hsa-miR-155-5p.

13.
J Transl Med ; 18(1): 392, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33059716

RESUMO

BACKGROUND: Intracranial aneurysms (IAs) are dangerous because of their potential to rupture. We previously found significant RNA expression differences in circulating neutrophils between patients with and without unruptured IAs and trained machine learning models to predict presence of IA using 40 neutrophil transcriptomes. Here, we aim to develop a predictive model for unruptured IA using neutrophil transcriptomes from a larger population and more robust machine learning methods. METHODS: Neutrophil RNA extracted from the blood of 134 patients (55 with IA, 79 IA-free controls) was subjected to next-generation RNA sequencing. In a randomly-selected training cohort (n = 94), the Least Absolute Shrinkage and Selection Operator (LASSO) selected transcripts, from which we constructed prediction models via 4 well-established supervised machine-learning algorithms (K-Nearest Neighbors, Random Forest, and Support Vector Machines with Gaussian and cubic kernels). We tested the models in the remaining samples (n = 40) and assessed model performance by receiver-operating-characteristic (ROC) curves. Real-time quantitative polymerase chain reaction (RT-qPCR) of 9 IA-associated genes was used to verify gene expression in a subset of 49 neutrophil RNA samples. We also examined the potential influence of demographics and comorbidities on model prediction. RESULTS: Feature selection using LASSO in the training cohort identified 37 IA-associated transcripts. Models trained using these transcripts had a maximum accuracy of 90% in the testing cohort. The testing performance across all methods had an average area under ROC curve (AUC) = 0.97, an improvement over our previous models. The Random Forest model performed best across both training and testing cohorts. RT-qPCR confirmed expression differences in 7 of 9 genes tested. Gene ontology and IPA network analyses performed on the 37 model genes reflected dysregulated inflammation, cell signaling, and apoptosis processes. In our data, demographics and comorbidities did not affect model performance. CONCLUSIONS: We improved upon our previous IA prediction models based on circulating neutrophil transcriptomes by increasing sample size and by implementing LASSO and more robust machine learning methods. Future studies are needed to validate these models in larger cohorts and further investigate effect of covariates.

14.
Sci Rep ; 10(1): 17432, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33060705

RESUMO

Lung cancer is the leading cause of cancer death worldwide. The Xuanwei-Fuyuan (XF) region of Yunnan, China has a high incidence of lung cancer from coal-related pollution. Effort to raise public awareness screening for lung cancer has been ongoing. We retrospectively analyzed overall survival (OS) of lung cancer patients of a tertiary cancer center in Yunnan to investigate screening and regional residential status as predictive factors. Consecutive cases of newly diagnosed lung cancer were reviewed. The lung cancer cases diagnosed by screening were more likely to be early-staged and treated by surgery than those diagnosed not by screening. In patients diagnosed not by screening, XF residential status was a significant predictor of improved OS. Frailty model detected significant heterogeneity associated with region of residence in unscreened patients. Potential biases associated with screening were examined by Monte Carlo simulations and sensitivity analyses. Focused effort in cancer screening and increased public awareness of pollution-related lung cancer in XF might have led to early diagnosis and improved OS, and increased investment in health care resources in high risk areas may have produced additional unobserved factors that underlay the association of XF residential status with improved OS in patients diagnosed not by screening.

15.
J Mol Cell Biol ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33064829

RESUMO

Necrosis, a type of unwanted and passive cell demise, usually occurs under the excessive external stress and is considered to be unregulated. However, under some special conditions such as caspase inhibition, necrosis is regulable in a well-orchestrated way. The term 'regulated necrosis' has been proposed to describe such programmed necrosis. Recently, several forms of necrosis, including necroptosis, pyroptosis, ferroptosis, parthanatos, oxytosis, NETosis, and Na+/K+-ATPase-mediated necrosis, have been identified, and some crucial regulators governing regulated necrosis have also been discovered. Mixed lineage kinase domain-like pseudokinase (MLKL), a core regulator in necroptosis, acts as an executioner in response to ligands of death receptor family. Its activation requires the receptor-interacting protein kinases, RIP1 and RIP3. However, MLKL is only involved in necroptosis, that is, MLKL is dispensable for necrosis. Therefore, this review is aimed at summarizing the molecular mechanisms of MLKL-dependent and MLKL-independent necrosis.

16.
Gait Posture ; 82: 322-328, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-33022565

RESUMO

OBJECTIVES: Although the overuse injury rate of the Achilles tendon (AT) for running is high, the effect of shoe conditions on AT loading remains unclear. Hence, this study aims to determine the mechanical properties of AT during running under different shoe conditions (minimalist vs. conventional shoes). DESIGN: This work is a controlled laboratory study. METHODS: Sixteen healthy male rearfoot strike runners were recruited to complete over ground running trials at 3.33 m/s (±5%) under two shoe conditions (minimalist shoes: INOV-8 Bare-XF 210; conventional shoes: NIKE AIR ZOOM PEGASUS 34). Sagittal plane ankle kinematics and ground reaction forces were simultaneously recorded. Ankle joint mechanics (ankle joint angle and moment) and the mechanical properties (peak force, impulse, stress, strain, and their corresponding peak rate) of the AT were calculated. RESULTS: In comparison with conventional shoes, wearing minimalist shoes showed significant changes (p < 0.05): (1) decreased strike angle (48.92 ±â€¯9.01 vs. 41.04 ±â€¯8.69°); (2) increased ankle moment (2.34 ±â€¯0.44 vs. 2.55 ±â€¯0.46 Nm/kg); (3) increased peak AT force (5.85 ±â€¯1.22 vs. 6.24 ±â€¯1.13 BW), AT force impulse (0.65 ±â€¯0.13 vs. 0.70 ±â€¯0.13 BW·s), peak AT loading rate (109.94 ±â€¯9.33 vs. 118.84 ±â€¯26.62 BW/s), and average loading rate (48.42 ±â€¯15.64 vs. 54.90 ±â€¯17.47 BW/s); (4) decreased time to peak AT force (126.31 ±â€¯20.68 vs. 117.77 ±â€¯17.62 ms); (5) increased AT stress (66.96 ±â€¯14.59 vs. 71.89 ±â€¯14.74 MPa), strain (8.19 ±â€¯1.77 vs. 8.78 ±â€¯1.80 %), peak AT stress rate (66.96 ±â€¯14.59 vs. 71.89 ±â€¯14.74 MPa/s), and strain rate (148.71 ±â€¯48.52 vs. 167.28 ±â€¯42.82 %/s). CONCLUSION: Increased AT force, loading rate, and stress were observed in runners who habitually wear conventional shoes with rearfoot strike patterns when they wore minimalist shoes. Hence, we recommend a gradual transition to minimalist shoes for runners who habitually wear conventional shoes with rearfoot strike patterns.

17.
Artigo em Inglês | MEDLINE | ID: mdl-32888301

RESUMO

AIMS: Hypertrophic cardiomyopathy (HCM) with left ventricular apical aneurysm (LVAA) is associated with an increased risk of adverse cardiovascular events. However, the clinical significance of LVAA in apical HCM (ApHCM) has not been reported. This study aimed to investigate the prevalence, cardiac magnetic resonance (CMR) characteristics, and prognosis of LVAA in ApHCM patients. METHODS AND RESULTS: A total of 1332 consecutive ApHCM patients confirmed by CMR in our hospital were retrospectively analysed. LVAAs were identified in 31 patients of all ApHCM patients (2.3%, 31/1332). Besides, 31 age- and gender-matched ApHCM patients without LVAA were used for comparison. Of the 31 aneurysm patients (mean age, 53.8 ± 15.1 years old), 28 (90.3%) had clinical symptoms, and 3 (9.7%) had a family history of HCM. The rate of missed diagnosis of echocardiography for detecting LVAA was 64.5% (20/31), most (90%, 18/20) of unidentified LVAAs by echocardiography were small aneurysms (<20 mm). Compared with ApHCM patients without LVAA, the proportion of systolic mid-cavity obstruction and late gadolinium enhancement (LGE) presence, and the LGE extent in ApHCM patients with LVAA were significantly higher (all P<0.05). The Kaplan-Meier curves showed that the event-free survival rate in ApHCM patients with LVAA was significantly lower than that in ApHCM patients without LVAA (log rank, P = 0.010). CONCLUSION: ApHCM with LVAA is a very rare condition, which is often missed by echocardiography and could be reliably detected with CMR and is associated with a higher risk of adverse cardiovascular events compared with ApHCM without LVAA.

18.
Clin Lab ; 66(9)2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32902212

RESUMO

BACKGROUND: MicroRNA-1207-5p (miR-1207-5p) has been identified as a tumor suppressor in many types of cancer. In our previous research, we found that the expression of miR-1207-5p in cancer tissues and cell lines were both down-regulated, and miR-1207-5p may function as tumor suppressor in colorectal cancer (CRC). However, less research has been done with respect to the expression of plasma miR-1207-5p in CRC or colorectal adenoma (CRA). There was no research regarding the diagnostic ability nor on the prognostic value of plasma miR-1207-5p in CRC. We conducted a preliminary study on the plasma miRNA-1207-5p as a non-invasive biomarker in CRC. METHODS: Plasma miR-1207-5p expression was quantified by qRT-PCR from CRC patients (n = 64), CRA patients (n = 42), and normal controls (n = 36). The blood samples were collected after having been diagnosed by pathology but before surgical resection and radio-chemotherapy. The CRC patients were divided into low and high expression groups according to the mean expression level of plasma miR-1207-5p. The relation of expression levels of miR-1207-5p and overall survival were analyzed by Kaplan-Meier survival curves. The receiver operating characteristic (ROC) curves were generated to assess the diagnostic ability of plasma miR-1207-5p in CRC. RESULTS: The expression of plasma miR-1207-5p was obvious down-regulated both in CRC patients (mean ± SD: 0.1661 ± 0.0083) and CRA patients (mean ± SD: 0.2480 ± 0.0162) compared to normal controls. The lower the ex-pression of plasma miR-1207-5p, the stronger the association with advanced TNM stage and positive lymph node metastasis compared with the high expression group (p = 0.027, p = 0.033). The lower the expressions of plasma miR-1207-5p, the shorter the overall survival time for CRC patients (p = 0.0404). There was no significant difference in the relative expression of plasma miR-1207-5p between the preoperative group and postoperative group. ROC analysis showed that the diagnostic sensitivity and specificity were 95.31% and 94.44% (at a cutoff = 0.2990) for CRC patients, 90.48% and 80.56% (at a cutoff = 0.4060) for CRA patients, respectively. The AUC value was 0.9852 (95% CI = 0.9870 - 1.0003, p < 0.0001), and 0.9530 (95% CI = 0.9117 - 0.9944, p < 0.0001), respectively. CONCLUSIONS: Significant down-regulation of plasma miR-1207-5p was correlated with poor survival and with strong diagnostic ability and may function as a potential biomarker for diagnosis and prognosis of colorectal cancer.

19.
Phys Rev Lett ; 125(8): 085902, 2020 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-32909783

RESUMO

Materials with high thermal conductivities (κ) are valuable to solve the challenge of waste heat dissipation in highly integrated and miniaturized modern devices. Herein, we report the first synthesis of atomically thin isotopically pure hexagonal boron nitride (BN) and its one of the highest κ among all semiconductors and electric insulators. Single atomic layer (1L) BN enriched with ^{11}B has a κ up to 1009 W/mK at room temperature. We find that the isotope engineering mainly suppresses the out-of-plane optical (ZO) phonon scatterings in BN, which subsequently reduces acoustic-optical scatterings between ZO and transverse acoustic (TA) and longitudinal acoustic phonons. On the other hand, reducing the thickness to a single atomic layer diminishes the interlayer interactions and hence umklapp scatterings of the out-of-plane acoustic (ZA) phonons, though this thickness-induced κ enhancement is not as dramatic as that in naturally occurring BN. With many of its unique properties, atomically thin monoisotopic BN is promising on heat management in van der Waals devices and future flexible electronics. The isotope engineering of atomically thin BN may also open up other appealing applications and opportunities in 2D materials yet to be explored.

20.
Artigo em Inglês | MEDLINE | ID: mdl-32920203

RESUMO

The vitellogenin receptor (Vgr), which is specific for vitellogenin (Vtg), recognises and transports Vtg into the ovaries. Accumulating evidence suggests that Vtg also performs an immune defence function and plays critical roles in innate immunity in oviparous animals. However, whether Vgr is involved in innate immunity in the Chinese mitten crab (Eriocheir sinensis) is unknown. In this study, we obtained a 3009 nucleotide partial cDNA of the E. sinensis vitellogenin receptor gene (Es-vgr) encoding an open reading frame of 1003 amino acid residues. Bioinformatics analysis showed that the domains of Es-vgr were conserved during evolution. Quantitative real-time PCR and western blotting revealed that the highest Es-vgr expression levels occurred in the ovary, and expression was specific. Comparison of the expression levels of Es-vgr and the Vtg gene (Es-vtg1) at different ovary developmental stages suggested that there may be some regulatory relationship between them. Bacterial challenge induced high-level expression of antimicrobial peptide genes and reduced Es-vgr expression in ovaries, resulting in massive accumulation of Vtg in the hemolymph. The survival rate of crabs increased significantly after injection with recombinant Es-vtg1 protein following bacterial infection. Collectively, these results demonstrate that Es-vgr plays critical roles in antimicrobial function by regulating the accumulation of Vtg in the hemolymph.

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