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1.
Am J Surg ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31818425

RESUMO

BACKGROUND: Optimal management for papillary thyroid microcarcinoma (PTMC) remains controversial. The purpose of this study was to explore risk factors predictive of cervical lymph node metastasis in conventional PTMCs. METHODS: Conventional PTMC patients (n = 2,404) undergoing surgery between 2010 and 2017 were grouped and analyzed according to the positivity of cervical lymph node. RESULTS: Central lymph node (CLN) metastases and lateral lymph node (LLN) metastases were observed in 915 (38.1%) and 184 (7.7%) cases, respectively. Multivariate analysis found that male (odds ratio [OR] = 1.974, p < 0.001), younger age (OR = 1.601, p < 0.001), tumor size (OR = 1.935, p < 0.001), extrathyroidal extension (ETE) (OR = 1.647, p < 0.001), multifocality (OR = 1.416, p < 0.001), and intrathyroidal spreading (OR = 3.355, p < 0.001) predicted increased CLN metastasis. In particular, younger age, multifocality, and intrathyroidal spreading were significantly associated with a high number of CLN metastases (n ≥ 5). The presence of CLN metastasis was strongly associated with LLN metastasis (OR = 5.426, p < 0.001). CONCLUSION: Male, younger age, tumor size, ETE, multifocality, and intrathyroidal spreading predict increased CLN metastasis in PTMCs. In patients with suspicious lateral lymphadenopathy, the presence of CLN metastasis is independently associated with LLN metastasis.

2.
Opt Express ; 27(17): 23682-23692, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31510269

RESUMO

Influenced by severe ambient noises and nonstationary disturbance signals, multi-class event classification is an enormous challenge in several long-haul application fields of distributed vibration sensing technology (DVS), including perimeter security, railway safety monitoring, pipeline surveillance, etc. In this paper, a deep dual path network is introduced into solving this problem with high learning capacity. The spatial time-frequency spectrum datasets are built by utilizing the multidimensional information of DVS signal, especially the spatial domain information. With the novel datasets and a high-parameter-efficiency network, the proposed scheme presents good reliability and robustness. The feasibility is verified in an actual railway safety monitoring field test, as a proof-of-concept. Seven types of real-life disturbances were implemented and their f1-scores all reached up to 97% in the test. The performance of this proposed approach is fully evaluated and discussed. The presented approach can be employed to improve the performance of DVS in actual applications.

3.
Int J Surg ; 60: 22-27, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30389533

RESUMO

BACKGROUND: After totally endoscopic thyroidectomy (TET), patients usually suffer from voice, sensory, swallowing symptoms and they are also concerned with the postoperative cosmetic result. We compared the effects of subfascial dissection method with the subplatysmal method in TET on postoperative symptoms and cervical appearance. METHODS: 143 female patients who underwent totally endoscopic thyroid surgery were assigned to the subplatysmal approach group and subfascial approach group. The patient's voice symptoms were assessed using the Voice Handicap Index (VHI) questionnaire. Sensory function was assessed with subjective pain and light touch sensation. Swallowing symptoms were assessed using the Swallow Impairment Score (SIS) questionnaire and the 3 oz water swallow test. Neck cosmetic effect was assessed by postoperative recovery time of the suprasternal fossa and subjective satisfaction questionnaire. Each variable was measured preoperatively, at 2 weeks, 3 months and 6 months after operation. RESULTS: In both groups, the subjective voice, sensory and swallowing symptoms deteriorated at 2 weeks and improved at 3 months postoperatively. Indicators of VHI, SIS and 3 oz water swallow test returned to the preoperative level at 6 months after operation. In the subfascial group, SIS was significantly better than that of the subplatysmal group (p = 0.005) at 2 weeks after operation. The recovery time of the suprasternal fossa in the subfascial group was delayed (p = 0.158), compared with the subplatysmal group. CONCLUSION: The subfascial approach may have certain advantages in swallowing symptoms basing on the swallowing impairment test. However, the subplatysmal approach and the subfascial approach may have the same cosmetic effect.


Assuntos
Endoscopia/métodos , Esvaziamento Cervical/métodos , Tireoidectomia/métodos , Adulto , Estudos de Coortes , Transtornos de Deglutição/epidemiologia , Transtornos de Deglutição/etiologia , Endoscopia/efeitos adversos , Fáscia , Feminino , Humanos , Pessoa de Meia-Idade , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Período Pós-Operatório , Estudos Prospectivos , Sensação/fisiologia , Sistema Musculoaponeurótico Superficial/cirurgia , Inquéritos e Questionários , Glândula Tireoide/cirurgia , Tireoidectomia/efeitos adversos , Resultado do Tratamento , Distúrbios da Voz/epidemiologia , Distúrbios da Voz/etiologia
4.
Biochem Biophys Res Commun ; 462(4): 314-21, 2015 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-25960292

RESUMO

Dedifferentiated thyroid carcinoma (DTC) with the loss of radioiodine uptake (RAIU) is often observed in clinical practice under radioiodine therapy, indicating the challenge for poor prognosis. MicroRNA (miRNA) has emerged as a promising therapeutic target in many diseases; yet, the role of miRNAs in RAIU has not been generally investigated. Based on recent studies about miRNA expression in papillary or follicular thyroid carcinomas, the expression profiles of several thyroid relative miRNAs were investigated in one DTC cell line, derived from normal DTC cells by radioiodine treatment. The top candidate miR-146b, with the most significant overexpression profiles in dedifferentiated cells, was picked up. Further research found that miR-146b could be negatively regulated by histone deacetylase 3 (HDAC3) in normal cells, indicating the correlation between miR-146b and Na(+)/I(-) symporter (NIS)-mediated RAIU. Fortunately, it was confirmed that miR-146b could regulate NIS expression/activity; what is more important, miR-146b interference would contribute to the recovery of radioiodine-sensitivity in dedifferentiated cells via positively regulating NIS. In the present study, it was concluded that NIS-mediated RAIU could be modulated by miR-146b; accordingly, miR-146b might serve as one of targets to enhance efficacy of radioactive therapy against poorly differential thyroid carcinoma (PDTC).


Assuntos
Diferenciação Celular , Radioisótopos do Iodo/farmacologia , MicroRNAs/genética , Simportadores/genética , Neoplasias da Glândula Tireoide/genética , Sequência de Bases , Primers do DNA , Histona Desacetilases/metabolismo , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Neoplasias da Glândula Tireoide/patologia
5.
J Phycol ; 49(6): 1181-91, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27007636

RESUMO

Full-length protein disulfide isomerase (UfPDI) cDNA was cloned from the intertidal macroalga Ulva lactuca Linnaeus. Modulation of UfPDI expression by stresses and polyamines (PA) was studied. UfPDI transcription and enzyme activity were increased by hypersalinity (90) or high light illumination (1,200 µmol photons · m(-2)  · s(-1) ), decreased by the addition of 100 µM CuSO4 . An exposure to a salinity of 90 decreased PA contents. Treating with PA biosynthetic inhibitors, D-arginine (D-Arg) or α-methyl ornithine (α-MO), led to a further decrease and also inhibited UfPDI expression and recovery of the growth rate. These results suggest that PAs are required to activate UfPDI expression with hypersalinity, even PA contents are decreased at a salinity of 90. The induction of UfPDI expression by hypersalinity of 90 and tolerance to hypersalinity could be enhanced if internal PA contents rise. Sung et al. (2011b) showed that PA contents could be increased by pretreating with putrescine (Put, 1 mM), spermidine (Spd, 1 mM), or spermine (Spm, 1 mM) at a salinity of 30. Therefore, PA pretreatment effect on UfPDI expression was examined. Pretreatment with Spd and Spm, but not with Put, enhanced UfPDI expression after transferred to a salinity of 90 and restored the growth rate. In conclusion, induction of UfPDI expression by Spd or Spm before exposure to hypersaline conditions and continuous up-regulation after hypersalinity exposure are required for the acquisition of hypersalinity tolerance in the intertidal green macroalga U. lactuca.

6.
Resuscitation ; 83(12): 1511-6, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22683500

RESUMO

AIMS: The cerebellum is among the brain regions most vulnerable to damage caused by cardiac arrest, and cerebellar Purkinje cell loss may contribute to neurologic dysfunction, including post-hypoxic myoclonus. However, it remains unknown whether cerebellar Purkinje cells are protected by post-cardiac arrest therapeutic hypothermia (TH). Therefore, we examined the effect of post-cardiac arrest TH onset and duration on cerebellar Purkinje cell loss. METHODS: Samples from a previously published study of post-cardiac arrest TH were utilized for the present analysis. Adult male rats subjected to asphyxial cardiac arrest and cardiopulmonary resuscitation were block randomized to normothermia (37.0°C) or TH (33.0°C) initiated 0, 1, 4, or 8h after return of spontaneous circulation (ROSC) and maintained for 24 or 48 h. Cerebella from rats surviving 7 days after ROSC were processed for histology and immunohistochemistry. Purkinje cell density was quantified in Nissl-stained sections of the primary fissure of the cerebellar vermis. RESULTS: With post-cardiac arrest normothermia, Purkinje cell density in the primary fissure was severely reduced compared to sham-injured controls (3.8 ± 1.8 cells mm(-1) vs. 35.9 ± 2.4 cells mm(-1), p<0.001). TH moderately improved Purkinje cell survival in all groups combined (14.0 ± 5.6 cells mm(-1), p<0.001 compared to normothermia). There was no statistical difference in Purkinje cell protection based on TH onset time or duration. CONCLUSION: These results indicate that post-cardiac arrest TH protects selectively vulnerable cerebellar Purkinje cells within a broad therapeutic window. The potential clinical implications for improving Purkinje cell survival require further investigation.


Assuntos
Cerebelo/patologia , Parada Cardíaca/patologia , Parada Cardíaca/terapia , Hipotermia Induzida , Células de Purkinje/patologia , Animais , Masculino , Ratos
7.
J Neurotrauma ; 29(2): 445-51, 2012 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-22077394

RESUMO

Traumatic axonal injury is characterized by early cytoskeletal proteolysis and disruption of axonal transport. Calpain inhibition has been shown to protect axons in rodent models of traumatic brain injury. However, in these models, both white and gray matter are injured, making it difficult to determine if calpain inhibitors are directly protecting injured axons. To address this issue, we used our rat optic nerve stretch model to test the hypothesis that early calpain inhibition directly protects central nervous system (CNS) axons following stretch injury. Rats were given an intravenous bolus of the calpain inhibitor MDL-28170 (30 mg/kg) 30 min prior to unilateral optic nerve stretch, followed by a 15 mg/kg/h intravenous infusion over the next 2.5 h. Immunohistochemical analysis of optic nerves 30 min after stretch injury revealed variable increases of calpain-cleaved α-spectrin that appeared less evident in stretched nerves from drug-treated rats, although this difference was not statistically significant. Retrograde axonal transport measured by Fluorogold® labeling of retinal ganglion cells was significantly impaired after stretch injury. However, there was no difference in the number of Fluorogold-labeled cells in the vehicle vs. drug treatment groups. These results suggest that early short-duration calpain inhibitor therapy with MDL-28170 is not an effective strategy to prevent disruption of axonal transport following isolated axonal stretch injury in the CNS.


Assuntos
Transporte Axonal/efeitos dos fármacos , Calpaína/antagonistas & inibidores , Inibidores de Cisteína Proteinase/administração & dosagem , Lesão Axonal Difusa/tratamento farmacológico , Dipeptídeos/farmacologia , Modelos Animais de Doenças , Animais , Transporte Axonal/fisiologia , Lesão Axonal Difusa/patologia , Lesão Axonal Difusa/fisiopatologia , Esquema de Medicação , Masculino , Fármacos Neuroprotetores/administração & dosagem , Ratos , Ratos Long-Evans , Fatores de Tempo , Falha de Tratamento
8.
Crit Care Med ; 39(6): 1423-30, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21610611

RESUMO

OBJECTIVE: Post-cardiac-arrest therapeutic hypothermia improves outcomes in comatose cardiac arrest survivors. This study tests the hypothesis that the efficacy of post-cardiac-arrest therapeutic hypothermia is dependent on the onset and duration of therapy. DESIGN: Prospective randomized laboratory investigation. SETTING: University research laboratory. SUBJECTS: A total of 268 male Long Evans rats. INTERVENTIONS: Post-cardiac-arrest therapeutic hypothermia. MEASUREMENTS AND MAIN RESULTS: Adult male Long Evans rats that achieved return of spontaneous circulation after a 10-min asphyxial cardiac arrest were block randomized to normothermia (37°C ± 1°C) or therapeutic hypothermia (33°C ± 1°C) initiated 0, 1, 4, or 8 hrs after return of spontaneous circulation and maintained for 24 or 48 hrs. Therapeutic hypothermia initiated 0, 1, 4, and 8 hrs after return of spontaneous circulation resulted in 7-day survival rates of 45%*, 36%*, 36%*, and 14%, respectively, compared to 17% for normothermic controls and survival with good neurologic function rates of 24%*, 24%*, 19%*, and 0%, respectively, compared to 2% for normothermic controls (*p < .05 vs. normothermia). These outcomes were not different when therapeutic hypothermia was maintained for 24 vs. 48 hrs. In contrast, hippocampal CA1 pyramidal neuron counts were 53% ± 27%*, 53% ± 19%*, 51% ± 24%*, and 65% ± 16%* of normal, respectively, when therapeutic hypothermia was initiated 0, 1, 4, or 8 hrs after return of spontaneous circulation compared to 9% in normothermic controls (*p < .01 vs. normothermia). Furthermore, surviving neuron counts were greater when therapeutic hypothermia was maintained for 48 hrs compared to 24 hrs (68% ± 15%* vs. 42% ± 22%, *p < .0001). CONCLUSIONS: In this study, post-cardiac-arrest therapeutic hypothermia resulted in comparable improvement of survival and survival with good neurologic function when initiated within 4 hrs after return of spontaneous circulation. However, histologic assessment of neuronal survival revealed a potentially broader therapeutic window and greater neuroprotection when therapeutic hypothermia was maintained for 48 vs. 24 hrs.


Assuntos
Região CA1 Hipocampal/patologia , Parada Cardíaca/complicações , Parada Cardíaca/terapia , Hipotermia Induzida , Doenças do Sistema Nervoso/prevenção & controle , Ressuscitação , Animais , Modelos Animais de Doenças , Parada Cardíaca/patologia , Masculino , Doenças do Sistema Nervoso/etiologia , Doenças do Sistema Nervoso/patologia , Ratos , Ratos Long-Evans , Fatores de Tempo
9.
Psychopharmacology (Berl) ; 212(3): 441-8, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20827462

RESUMO

RATIONALE: Parenteral injections of d-lysergic acid diethylamide (LSD), a serotonin 5-HT(2A) receptor agonist, enhance eyeblink conditioning. Another hallucinogen, (±)-1(2, 5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI), was shown to elicit a 5-HT(2A)-mediated behavior (head bobs) after injection into the hippocampus, a structure known to mediate trace eyeblink conditioning. OBJECTIVE: This study aims to determine if parenteral injections of the hallucinogens LSD, d,l-2,5-dimethoxy-4-methylamphetamine, and 5-methoxy-dimethyltryptamine elicit the 5-HT(2A)-mediated behavior of head bobs and whether intrahippocampal injections of LSD would produce head bobs and enhance trace eyeblink conditioning. MATERIALS AND METHODS: LSD was infused into the dorsal hippocampus just prior to each of eight conditioning sessions. One day after the last infusion of LSD, DOI was infused into the hippocampus to determine whether there had been a desensitization of the 5-HT(2A) receptor as measured by a decrease in DOI-elicited head bobs. RESULTS: Acute parenteral or intrahippocampal LSD elicited a 5-HT(2A) but not a 5-HT(2C)-mediated behavior, and chronic administration enhanced conditioned responding relative to vehicle controls. Rabbits that had been chronically infused with 3 or 10 nmol per side of LSD during Pavlovian conditioning and then infused with DOI demonstrated a smaller increase in head bobs relative to controls. CONCLUSIONS: LSD produced its enhancement of Pavlovian conditioning through an effect on 5-HT(2A) receptors located in the dorsal hippocampus. The slight, short-lived enhancement of learning produced by LSD appears to be due to the development of desensitization of the 5-HT(2A) receptor within the hippocampus as a result of repeated administration of its agonist (LSD).


Assuntos
2,5-Dimetoxi-4-Metilanfetamina/farmacologia , Dietilamida do Ácido Lisérgico/farmacologia , Metoxidimetiltriptaminas/farmacologia , Receptor 5-HT2A de Serotonina/efeitos dos fármacos , 2,5-Dimetoxi-4-Metilanfetamina/administração & dosagem , Animais , Piscadela/efeitos dos fármacos , Condicionamento Clássico/efeitos dos fármacos , Alucinógenos/administração & dosagem , Alucinógenos/farmacologia , Movimentos da Cabeça/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Injeções , Aprendizagem/efeitos dos fármacos , Dietilamida do Ácido Lisérgico/administração & dosagem , Metoxidimetiltriptaminas/administração & dosagem , Coelhos , Receptor 5-HT2A de Serotonina/metabolismo , Receptor 5-HT2C de Serotonina/efeitos dos fármacos , Agonistas do Receptor de Serotonina/administração & dosagem , Agonistas do Receptor de Serotonina/farmacologia
10.
Exp Neurol ; 224(1): 170-7, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20298691

RESUMO

The calpain family of cysteine proteases has a well-established causal role in neuronal cell death following acute brain injury. However, the relative contribution of calpain isoforms has not been determined in in vivo models. Identification of the calpain isoform responsible for neuronal injury is particularly important given the differential role of calpain isoforms in normal physiology. This study evaluates the role of m-calpain and micro-calpain in an in vivo model of global brain ischemia. Adeno-associated viral vectors expressing short hairpin RNAs targeting the catalytic subunits of micro- or m-calpain were used to knockdown expression of the targeted isoforms in adult rat hippocampal CA1 pyramidal neurons. Knockdown of micro-calpain, but not m-calpain, prevented calpain activity 72 h after 6-min transient forebrain ischemia, increased long-term survival and protected hippocampal electrophysiological function. These findings represent the first in vivo evidence that reducing expression of an individual calpain isoform can decrease post-ischemic neuronal death and preserve hippocampal function.


Assuntos
Isquemia Encefálica/fisiopatologia , Calpaína/genética , Sobrevivência Celular/genética , Hipocampo/fisiologia , Neurônios/fisiologia , Análise de Variância , Animais , Isquemia Encefálica/genética , Células Cultivadas , Dependovirus , Eletrofisiologia , Imunofluorescência , Vetores Genéticos/genética , Hipocampo/citologia , Masculino , Neurônios/citologia , Isoformas de Proteínas/genética , Interferência de RNA , RNA Interferente Pequeno/genética , Ratos
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