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1.
BMC Cancer ; 20(1): 261, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-32228516

RESUMO

BACKGROUND: S100 calcium binding protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. METHODS: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of S100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by shRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT, colony formation assay, transwell assay,flow cytometry assa and western blot. RESULTS: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. CONCLUSION: S100A12 plays a vital role in glioma progression, and may be an important regulatory molecule for biological behaviors of glioma cell lines.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32239154

RESUMO

The back-n project in China Spallation Neutron Source (CSNS) was launched primarily for nuclear data measurements. In the backscattering neutron hall, the neutron and gamma monitors were used for dose monitoring. Because of the dead time problem of monitors, performance of the monitors in such pulsed radiation field needs to be analyzed. In this research, experiments with dose monitors and personal dosemeters were conducted, and simulation by Monte Carlo code FLUKA was performed. Results showed that the values by monitors are smaller, and the larger the dose, the larger the difference. The reasons in term of energy response and dead time have been analyzed, and corrections were discussed. After corrections, the measured value can agree with the simulation results in the range of about a factor 3. Totally speaking, the values recorded by neutron and gamma monitors can be a reference for radiation safety management in CSNS.

3.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 42(1): 55-61, 2020 Feb 28.
Artigo em Chinês | MEDLINE | ID: mdl-32131940

RESUMO

Objective To investigate the clinical values of nutritional status and chest CT phenotypes in the assessment of chronic obstructive pulmonary disease(COPD). Methods A total of 256 patients with stable COPD were enrolled from Peking Union Medical College Hospital and Civil Aviation General Hospital from June 2017 to June 2018.Demographic data,height,weight,smoking history,and number of exacerbations were collected.Pulmonary function tests and COPD assessment test(CAT)questionnaire-based survey were performed.The correlations of Goddard score with pulmonary function,CAT score,and number of exacerbations were analyzed.The clinical features of COPD patients with different body mass index(BMI)grades and CT phenotype were analyzed. Results The forced expiratory volume in one second as percentage of predicted value(FEV1%pred)was significantly higher in normal body mass group(t=-2.701,P=0.0080),overweight group(t=-3.506,P=0.001),and obese group(t=-4.323,P=0.000)than in low body mass group and was significantly higher in obese group than in normal body mass group(t=-3.096,P=0.002).The forced vital capacity as percentage of predicted value(FVC%pred)of normal body mass group(t=-3.081,P=0.002)and overweight group(t=-2.766,P=0.006)were significantly higher than that of low body mass group.The forced expiratory volume in one second(FEV1)/forced vital capacity(FVC)was significantly higher in overweight group than in normal body mass group(t=-3.702,P=0.001)and significantly higher in obese group than in low body mass group(t=-4.742,P=0.000),normal body mass group(t=-5.785,P=0.000),and overweight group(t=-2.984,P=0.003).In addition,the carbon monoxide diffusing capacity as percentage of predicted value(DLco%pred)was significantly higher in overweight group than in underweight(t=-3.042,P=0.003)and normal body mass groups(t=-3.128,P=0.002)and significantly higher in obese group than in underweight group(t=-4.742,P=0.000)and normal body mass group(t=-5.785,P=0.000).The Goddard scores of overweight(Z=4.535,P=0.000)and obese groups(Z=5.422,P=0.000)were significantly lower than that of normal body mass group.Partial correlation analysis showed that Goddard score was negatively correlated with FEV1/FVC(r =-0.230,P = 0.022)and DLco%pred(r =-0.531,P = 0.000)and positively correlated with CAT score(r = 0.244,P = 0.021).BMI of phenotype E(t=3.467,P=0.001)and M(t=3.031,P=0.003),FEV1/FVC of phenotype E(t=2.484,P=0.015)and M(t=2.969,P=0.004)as well as DLco%pred of phenotype E(t=4.928,P=0.000)and M(t=2.489,P=0.0163)were significantly lower than those of phenotype A.Patients with phenotype M had worse FEV1%pred,FVC%pred,residual volume/total lung capacity and number of acute exacerbations than patients with phenotypes A and E,but the differences were not statistically significant(all P >0.05). Conclusions The nutritional status is closely related to lung function,severity of emphysema,and number of exacerbations in COPD patients.Chest CT phenotype is clinically valuable in the assessment of COPD.


Assuntos
Índice de Massa Corporal , Doença Pulmonar Obstrutiva Crônica/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Volume Expiratório Forçado , Humanos , Pulmão/fisiopatologia , Obesidade/patologia , Sobrepeso/patologia , Radiografia Torácica , Testes de Função Respiratória
4.
Zhongguo Zhen Jiu ; 40(2): 185-91, 2020 Feb 12.
Artigo em Chinês | MEDLINE | ID: mdl-32100506

RESUMO

OBJECTIVE: To explore the effect of electroacupuncture (EA) on insulin sensitivity, adipose tissue inflammatory reaction and silent information regulation factor 1(SIRT1)/nuclear factor kappa B (NF-κB) signaling pathway in obese rats. METHODS: A total of 100 SPF-grade Wistar male rats were collected. Thirteen rats of them were selected randomly as the normal group and fed with common forage, and the rest rats were fed with high-fat forage. Eight weeks later, 39 rats that met the obesity criteria were randomized into a model group, an EA group and a sham-EA group, 13 rats in each one. In each group, 3 rats were collected randomly and the hyperinsulinemic-euglycemic clamp was exerted to record glucose infusion rate (GIR) so as to determine insulin sensitivity. Afterwards, in the EA group, EA was applied to "Zusanli" (ST 36), "Fenglong" (ST 40), "Zhongwan" (CV 12) and "Guanyuan" (CV 4), stimulated with continuous wave, 2 Hz in frequency, 1 mA in current intensity, for 15 min. The treatment was given once every 2 days, 3 times a week, for 8 weeks totally. In the sham-EA group, the needles were inserted shallowly at the sites, 5 mm lateral to each of the acupoints stimulated in the EA group, and the electrodes were attached to the needle handles, but without electric stimulation exerted. The rest management was the same as the EA group. Before and after intervention, the body mass and the insulin sensitivity were measured. After intervention, the white adipose tissue was collected from the kidney in the rats. Western blot was adopted to detect the relative protein expressions of SIRT1, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and acetylated NF-κB (Ac-NFκB). The real-time fluorescence quantitative PCR was used to detect the mRNA expressions of SIRT1, IL-6 and TNF-α. The immunofluorescence double labeling method was applied to detect the co-expression of SIRT1 and Ac-NFκB in adipose tissue. RESULTS: After fed with high-fat forage for 8 weeks, the body mass was significantly increased and GIR decreased in the rats of the model group as compared with the normal group (P<0.01), suggesting that the model of obese rat with insulin resistance was successfully established. After 8-week intervention, compared with the model group, the body mass was reduced and GIR increased in the rats of the EA group (P<0.01). The differences were not significant statistically in comparison between the sham-EA group and the model group (P>0.05). Compared with the normal group, in the model group, the protein and mRNA expressions of SIRT1 in adipose tissue were decreased, and the protein expression of Ac-NFκB increased, the protein and mRNA expressions of IL-6 and TNF-α increased (P<0.05, P<0.01). Compared with the model group, in the EA group, the protein and mRNA expressions of SIRT1 in adipose tissue were increased significantly, the protein expression of Ac-NFκB decreased, and the protein and mRNA expressions of IL-6 and TNF-α significantly decreased (P<0.05, P<0.01). There was no significant difference in each index between the sham-EA group and the model group (P>0.05). The results of immunofluorescence double labeling showed that SIRT1 and Ac-NFκB were co-expressed in adipose tissue. CONCLUSION: Electroacupuncture significantly reduces the body mass, inflammatory reaction conditions of adipose tissue and improves insulin sensitivity in obese rats. Regarding the potential mechanism, after the activation of SIRT1/NF-κB signaling pathway by electroacupuncture, and down-regulates the transcription of downstream inflammatory factors.


Assuntos
Tecido Adiposo/metabolismo , Eletroacupuntura , NF-kappa B/metabolismo , Obesidade/patologia , Transdução de Sinais , Sirtuína 1/metabolismo , Pontos de Acupuntura , Animais , Interleucina-6/metabolismo , Masculino , Distribuição Aleatória , Ratos , Ratos Wistar , Fator de Necrose Tumoral alfa/metabolismo
5.
Nano Lett ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32013439

RESUMO

Engineering the anomalous Hall effect (AHE) is the key to manipulate the magnetic orders in the emerging magnetic topological insulators (MTIs). In this letter, we synthesize the epitaxial Bi2Te3/MnTe magnetic heterostructures and observe pronounced AHE signals from both layers combined together. The evolution of the resulting hybrid AHE intensity with the top Bi2Te3 layer thickness manifests the presence of an intrinsic ferromagnetic phase induced by the topological surface states at the heterolayer-interface. More importantly, by doping the Bi2Te3 layer with Sb, we are able to manipulate the sign of the Berry phase-associated AHE component. Our results demonstrate the un-paralleled advantages of MTI heterostructures over magnetically doped TI counterparts, in which the tunability of the AHE response can be greatly enhanced. This in turn unveils a new avenue for MTI heterostructure-based multifunctional applications.

6.
Can J Aging ; : 1-13, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32037994

RESUMO

The psychological well-being of family caregivers is influenced by their relations with care receivers, and whether they have choice in becoming a caregiver. Limited study has explored the interaction effect of caregiver-receiver relations and caregiving choice on caregivers' psychological well-being. This study examines whether the caregiver's perceived choice moderates the association between caregiver-receiver relation and psychological well-being. Using population-based data from the 2012 Canada General Social Survey - Caregiving and Care Receiving (n = 5,285), this study applies regression and ANCOVA analyses. Results show family caregivers for spouses and children report significantly worse psychological well-being, whereas having choice to become a caregiver is associated with better psychological well-being. There was a significant moderation effect of caregiving choice on the association between caregiver-receiver relation and psychological well-being. Findings suggest that more services should be targeted for family caregivers without choice for caregiving as well as those who provide care for their children.

7.
J Neuroinflammation ; 17(1): 73, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32098620

RESUMO

BACKGROUND: The recruitment of immune system cells into the central nervous system (CNS) has a profound effect on the outcomes of injury and disease. Glia-derived chemoattractants, including chemokines, play a pivotal role in this process. In addition, cytokines and chemokines influence the phenotype of infiltrating immune cells. Depending on the stimuli present in the local milieu, infiltrating macrophages acquire the classically activated M1 or alternatively activated M2 phenotypes. The polarization of macrophages into detrimental M1 versus beneficial M2 phenotypes significantly influences CNS pathophysiology. Earlier studies indicated that a toll-like receptor 9 (TLR9) antagonist modulates astrocyte-derived cytokine and chemokine release. However, it is not known whether these molecular changes affect astrocyte-induced chemotaxis and polarization of macrophages. The present studies were undertaken to address these issues. METHODS: The chemotaxis and polarization of mouse peritoneal macrophages by spinal cord astrocytes were evaluated in a Transwell co-culture system. Arrays and ELISA were utilized to quantify chemokines in the conditioned medium (CM) of pure astrocyte cultures. Immunostaining for M1- and M2-specific markers characterized the macrophage phenotype. The percentage of M2 macrophages at the glial scar was determined by stereological approaches in mice sustaining a mid-thoracic spinal cord contusion injury (SCI) and intrathecally treated with oligodeoxynucleotide 2088 (ODN 2088), the TLR9 antagonist. Statistical analyses used two-tailed independent-sample t-test and one-way analysis of variance (ANOVA) followed by Tukey's post hoc test. A p value < 0.05 was considered to be statistically significant. RESULTS: ODN 2088-treated astrocytes significantly increased the chemotaxis of peritoneal macrophages via release of chemokine (C-C motif) ligand 1 (CCL1). Vehicle-treated astrocytes polarized macrophages into the M2 phenotype and ODN 2088-treated astrocytes promoted further M2 polarization. Reduced CCL2 and CCL9 release by astrocytes in response to ODN 2088 facilitated the acquisition of the M2 phenotype, suggesting that CCL2 and CCL9 are negative regulators of M2 polarization. The percentage of M2 macrophages at the glial scar was higher in mice sustaining a SCI and receiving ODN 2088 treatment as compared to vehicle-treated injured controls. CONCLUSIONS: TLR9 antagonism could create a favorable environment during SCI by supporting M2 macrophage polarization and chemotaxis via modulation of astrocyte-to-macrophage signals.

8.
ACS Appl Mater Interfaces ; 12(1): 706-716, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31799821

RESUMO

Rational design and convenient preparation of freestanding, hierarchical, and porous composites consisting of three-dimensional (3D) conductive carbon and low-dimension nanostructures with well-defined morphology and direct application as electrodes in rechargeable batteries are challenging. Herein, a freestanding, hierarchical, and porous composite composed of bilayered NaxV2O5·nH2O(NVO) nanobelts, carbon nanotubes (CNTs), and reduced graphene oxide (rGO) with a 3D cross-linked structure is prepared by simple one-pot hydrothermal self-assembly and vacuum filtration. The unique hierarchical nanoarchitecture of the hybrid one-dimensional (1D) (NVO nanobelts) and 3D (rGO/CNT) scaffold provides efficient pathways for ion/electron transportation as well as an elastic medium to buffer large volume change of the bilayered NVO nanobelts during cycling. The materials have excellent electrochemical properties as cathodes in the nonaqueous K-ion batteries (KIBs) and aqueous zinc-ion batteries (AZIBs). In the nonaqueous KIBs, the freestanding composite exhibits a high capacity of 119.9 mA h g-1 at 0.1 A g-1, a rate capability of 53.2 mA h g-1 at 3 A g-1, and a superior cycling stability of 59.7 mA h g-1 at 0.5 mA g-1 after 600 cycles. In the AZIBs, a capacity of 459.1 mA h g-1 at 0.5 A g-1, a rate capability of 352.5 mA h g-1 at 10 A g-1, and 83.1% retention after 1800 cycles at 10 A g-1 are observed. Our results reveal the advantages of the 3D rGO/CNT platform for 1D vanadium-based oxide nanostructures and provide insights into the design and preparation of efficient cathode materials in high-performance AZIBs and nonaqueous KIBs.

9.
Genomics ; 112(2): 1128-1138, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31251979

RESUMO

Cold stress imposes a great impact on the growth of nearly all photosynthetic organisms, including Chlamydomonas reinhardtii (C. reinhardtii). Despite prior studies on the mechanism of stress acclimation in plants, little has been done on the early events of cold sensing in C. reinhardtii. Here, we used C. reinhardtii as a model to study early events of cold signal transduction. By analyzing transcriptomic changes of C. reinhardtii exposed to cold, we found that 3471 genes were differentially expressed after 1 h of cold exposure. These genes were associated with a wide range of biological events and processes such as protein synthesis, cell cycle and protein kinase-based phosphorylation. Besides, the promoter of one gene (named as crAP2) which belongs to AP2/EREBP family and was significantly induced by cold was cloned, and functional analysis was conducted using GUS activity analysis through Agrobacterium-mediated transient assay in tobacco leaves.

10.
Eur J Surg Oncol ; 46(1): 65-70, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31519428

RESUMO

BACKGROUND: The current National Practice Questionnaire of implant-based breast reconstruction (IBBR) (NPQi) was to assess the clinical practice of mesh-assisted IBBR in China. METHODS: A questionnaire was mailed to 110 hospitals in China, which have more than 200 breast cancer operations performed in 2017. The survey mainly included questions on the type and timing of IBBR, questions about the use of TiLOOP® Bra and acellular dermal matrix (ADM) and the complications of IBBR. RESULTS: IBBR was routinely carried out in 86.36% (95/110) hospitals. IBBR was the most frequently-used (65.7%, 4,296/6,534) BR after mastectomy with a median of 24 cases (IQR 7.5-65) in each hospital. TiLOOP® Bra and ADM were available in 49.5% and 33.7% hospitals, respectively. Hospitals with ADM offered were more likely to located in economically developed regions (65.6%), when compared with hospitals without any mesh offered (14/35, 40.0%, P = 0.036) and with only TiLOOP® Bra offered (16/28, 57.1%, P = 0.032). The surgery volume was largely variated from hospitals without any mesh offered (median 380 cases, IQR 304-550), with only TiLOOP® Bra offered (median 790 cases, IQR 439-1096, P = 0.001) and with ADM offered (median 797 cases, IQR 497-1528, P < 0.001). Higher proportion of one-stage mesh-augmented direct-to-implant BR and lower proportion of autologous BR were observed in hospitals with mesh offered. The reported major complications were similar between hospitals with or without mesh offered. CONCLUSIONS: The NPQi has provided a valuable insight into the current practice of IBBR and mesh used in China. The introduction of mesh-assisted techniques has revolutionized the clinical practice.

11.
J Hazard Mater ; 381: 120916, 2020 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-31387075

RESUMO

Toxic gases released from lithium-ion battery (LIB) fires pose a very large threat to human health, yet they are poorly studied, and the knowledge of LIB fire toxicity is limited. In this paper, the thermal and toxic hazards resulting from the thermally-induced failure of a 68 Ah pouch LIB are systematically investigated by means of the Fourier transform infrared spectroscopy (FTIR) and 1/2 ISO full scale test room. The LIBs with higher state of charge (SOC) are found to have greater fire risks in terms of their burning behavior, normalized heat release rate, and fire radiation, as well as the concentration of toxic gases. Specifically, the thermal hazards are evaluated by combining the effects of convective and radiative heat. The major toxic gases detected from the online analysis are CO, HF, SO2, NO2, NO and HCl. Furthermore, Fractional Effective Dose (FED) and Fractional Effective Concentration (FEC) models are used to quantitatively assess the overall gas toxicity. Results show that the effects of irritant gases are much more significant than those of asphyxiant gases. HF and SO2 have much greater toxicity than the other fire gases. The maximum FEC value is approaching the critical threshold in such fire scenarios.

12.
Mol Cancer ; 18(1): 187, 2019 12 19.
Artigo em Inglês | MEDLINE | ID: mdl-31856843

RESUMO

BACKGROUND: The majority of breast cancer patients die of metastasis rather than primary tumors, whereas the molecular mechanisms orchestrating cancer metastasis remains poorly understood. Long noncoding RNAs (lncRNA) have been shown to regulate cancer occurrence and progression. However, the lncRNAs that drive metastasis in cancer patients and their underlying mechanisms are still largely unknown. METHODS: lncRNAs highly expressed in metastatic lymph nodes were identified by microarray. Survival analysis were made by Kaplan-Meier method. Cell proliferation, migration, and invasion assay was performed to confirm the phenotype of LINC02273. Tail vein model and mammary fat pad model were used for in vivo study. RNA pull-down and RIP assay were used to confirm the interaction of hnRNPL and LINC02273. Chromatin isolation by RNA purification followed by sequencing (ChIRP-seq), RNA-seq, ChIP-seq, and luciferase reporter assay reveal hnRNPL-LINC02273 regulates AGR2. Antisense oligonucleotides were used for in vivo treatment. RESULTS: We identified a novel long noncoding RNA LINC02273, whose expression was significantly elevated in metastatic lesions compared to the primary tumors, by genetic screen of matched tumor samples. Increased LINC02273 promoted breast cancer metastasis in vitro and in vivo. We further showed that LINC02273 was stabilized by hnRNPL, a protein increased in metastatic lesions, in breast cancer cells. Mechanistically, hnRNPL-LINC02273 formed a complex which activated AGR2 transcription and promoted cancer metastasis. The recruitment of hnRNPL-LINC02273 complex to AGR2 promoter region epigenetically upregulated AGR2 by augmenting local H3K4me3 and H3K27ac levels. Combination of AGR2 and LINC02273 was an independent prognostic factor for predicting breast cancer patient survival. Moreover, our data revealed that LINC02273-targeting antisense oligonucleotides (ASO) substantially inhibited breast cancer metastasis in vivo. CONCLUSIONS: Our findings uncover a key role of LINC02273-hnRNPL-AGR2 axis in breast cancer metastasis and provide potential novel therapeutic targets for metastatic breast cancer intervention.

13.
BMJ Open ; 9(12): e032945, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31843846

RESUMO

INTRODUCTION: Available patient-reported outcome (PRO) studies are mainly from single institution or of small sample size, and the variations across hospitals and regions were not fully analysed. A multicentre, prospective, patient-reported outcome-reconstruction and oncoplastic cohort (PRO-ROC) will be planned to assess the PROs of Chinese patients with breast cancer who will undergo breast reconstruction (BR) or oncoplastic breast-conserving surgery (OBCS). METHODS AND ANALYSIS: The inclusion criteria are female patients with breast cancer aged >18 years old who will undergo BR or OBCS. This cohort will include at least 10 000 consecutive patients (about 5000 patients who will undergo BR and 5000 patients who will undergo OBCS). The exposures were surgery types: BR and OBCS regardless of the techniques and materials used. The primary endpoint will be PROs, which include BREAST-Q and quality of life (European Organisation for Research and Treatment (EORTC) Quality of Life Questionnaire Core 30 (QLQ-C30) and EORTC QoL Breast Cancer-specific version (QLQ-BR23)). All patients will be followed up to 24 months after operations. All data will be prospectively collected using an app software. Data will be analysed using SPSS and Stata software. ETHICS AND DISSEMINATION: This study follows the Helsinki Declaration. All patients will be asked to sign an informed consent before enrolment. The results of this study will be presented at national and international meetings and published in a scientific peer-reviewed journal. TRIAL REGISTRATION NUMBER: NCT04030845; Pre-results.

14.
Opt Express ; 27(21): 30441-30448, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31684291

RESUMO

Parametric spectro-temporal analyzer (PASTA) has been demonstrated as a powerful tool for ultrafast spectrum measurement with superior frame rate and resolution. Compared with other time-stretch-based counterparts, the temporal focusing mechanism enlarges the initial condition and enables the observation of arbitrary waveform, especially the emission spectrum. However, due to the limited conversion bandwidth of the parametric mixing-based time-lens, the observation bandwidth of PASTA is constrained within the C (conventional) band, which hinders its practical applications. To overcome this constraint, both stokes and anti-stokes conversions of the parametric mixing process are leveraged, and the concept of time division multiplexing (TDM) is introduced to ensure their separability. Therefore, the TDM-based PASTA system successfully demultiplexes the C band and L (long) band spectra in two adjacent temporal frames. It is capable of reconstructing the wavelength-to-time sequence for arbitrary waveform over a record 58-nm observation bandwidth, which can be further improved by optimizing the filters and amplifiers. Meanwhile, both of these two bands achieve 20-pm resolution, 10-MHz frame rate, and -30-dBm sensitivity. Moreover, this TDM concept can also be applied to other parametric mixing-based temporal imaging systems to enlarge the working wavelength band, such as temporal magnification.

15.
J Neuroinflammation ; 16(1): 207, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703709

RESUMO

BACKGROUND: Neuropathic pain is often observed in individuals with multiple sclerosis (MS) and spinal cord injury (SCI) and is not adequately alleviated by current pharmacotherapies. A better understanding of underlying mechanisms could facilitate the discovery of novel targets for therapeutic interventions. We previously reported that decreased plasma membrane calcium ATPase 2 (PMCA2) expression in the dorsal horn (DH) of healthy PMCA2+/- mice is paralleled by increased sensitivity to evoked nociceptive pain. These studies suggested that PMCA2, a calcium extrusion pump expressed in spinal cord neurons, plays a role in pain mechanisms. However, the contribution of PMCA2 to neuropathic pain processing remains undefined. The present studies investigated the role of PMCA2 in neuropathic pain processing in the DH of wild-type mice affected by experimental autoimmune encephalomyelitis (EAE), an animal model of MS, and following SCI. METHODS: EAE was induced in female and male C57Bl/6N mice via inoculation with myelin oligodendrocyte glycoprotein fragment 35-55 (MOG35-55) emulsified in Complete Freund's Adjuvant (CFA). CFA-inoculated mice were used as controls. A severe SC contusion injury was induced at thoracic (T8) level in female C57Bl/6N mice. Pain was evaluated by the Hargreaves and von Frey filament tests. PMCA2 levels in the lumbar DH were analyzed by Western blotting. The effectors that decrease PMCA2 expression were identified in SC neuronal cultures. RESULTS: Increased pain in EAE and SCI was paralleled by a significant decrease in PMCA2 levels in the DH. In contrast, PMCA2 levels remained unaltered in the DH of mice with EAE that manifested motor deficits but not increased pain. Interleukin-1ß (IL-1ß), tumor necrosis factor α (TNFα), and IL-6 expression were robustly increased in the DH of mice with EAE manifesting pain, whereas these cytokines showed a modest increase or no change in mice with EAE in the absence of pain. Only IL-1ß decreased PMCA2 levels in pure SC neuronal cultures through direct actions. CONCLUSIONS: PMCA2 is a contributor to neuropathic pain mechanisms in the DH. A decrease in PMCA2 in DH neurons is paralleled by increased pain sensitivity, most likely through perturbations in calcium signaling. Interleukin-1ß is one of the effectors that downregulates PMCA2 by acting directly on neurons.

16.
Gland Surg ; 8(5): 477-485, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31741878

RESUMO

Background: Internal mammary vessels (IMVs) are widely used recipient vessels in abdominal free flap breast reconstructions. Rib sparing technique is an alternative method with less damage in IMVs exposure. This study aims to investigate the factors influencing the selection of IMVs, as well as analyze the applicability and related factors of rib sparing technique in abdominal breast reconstruction. Methods: Medical records of 215 patients who underwent abdominal free flap reconstruction from November 2006 to December 2017 in Fudan University Shanghai Cancer Center (FUSCC) were analyzed. Intercostal space (ICS) was measured from preoperative chest computed tomography scan. Factors influencing the choice of recipient vessels and rib sparing were analyzed. Surgery time, hospitalization and complications were assessed. Results: Among all 218 flaps, 172 flaps used IMVs as the recipient vessels while 46 used other vessels. patients with immediate reconstruction (P=0.005) and axillary lymph nodes dissection (ALND) (P<0.001) were less likely to use IMVs. Patients' body mass index (BMI) and radiotherapy history showed no statistically significant differences between the two groups (P=0.338 and 0.811). In IMVs group, 62% cases used rib sparing technique. Compared with rib resection group, patients with rib sparing were taller (P=0.047) and with a wider ICS (2.65±0.54 vs. 2.25±0.38 cm, P<0.001). Rib sparing group had a shorter surgery and postoperative hospitalization time, as well as a lower complication rate, but the differences were not statistically significant (P=0.120, 0.450 and 0.612). Conclusions: IMVs were used more frequently as the recipient vessels in abdominal free flap breast reconstructions, especially when axillary operation was not performed at the same time. Rib sparing technique had the potential to decrease surgery time, hospitalization days and complications rate. It could be applied in most of the patients with IMVs exposure, particularly in taller patients and patients with a wider ICS. Preoperative chest computed tomography scan can be used to assess the ICS width to provide operational suggestions.

17.
Micromachines (Basel) ; 10(11)2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31766260

RESUMO

A calibration method for the designed curved zoom compound eye is studied in order to achieve detection and positioning of spatial objects. The structure of the curved zoom compound eye is introduced. A calibration test platform is designed and built based on the image characteristics of the compound eye, which can be constructed in the large field view for the calibration target. The spot images are obtained through image processing. The center of the spot is calculated by Gauss fitting method. This method is highly simple and intuitive, and it can be used in a zoom surface compound eye without any complex procedures. Finally, the corresponding relationship between the spot center coordinates and the incident light vector of the corresponding sub-eye is established, and the calibration of the multi vision positioning system is completed.

18.
Theranostics ; 9(23): 6840-6855, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31660072

RESUMO

Background: Chemotherapy resistance is a major problem in breast cancer treatment and a leading cause of mortality in breast cancer patients. Biomarkers for chemotherapy resistance is under investigation. Methods: Paclitaxel resistant cells were established and subjected to RNA sequencing. Analysis combined with two additional RNA-seq datasets was conducted. CapG expression in patients with adjuvant chemotherapy was studied in breast cancer resection specimens using IHC and related to pathological response and disease-free survival. Paclitaxel resistance was assessed by half-maximal inhibitory concentrations (IC50) and a mouse xenograft model. Results: Increased expression of actin-binding protein CapG strongly correlated with the resistance to paclitaxel chemotherapy and decreased probability to achieve pathological complete response in breast cancer patients. Overexpressing CapG significantly enhanced paclitaxel resistance in breast cancer cells and xenograft tumors. High CapG level also significantly correlated with shorter relapse-free survival as well as hyper-activation of PI3K/Akt signaling in breast cancer patients. Mechanistically, CapG enhanced PIK3R1 expression which led to increased PI3K/Akt activation. Unexpectedly, CapG was found to bind to the variant-specific promoter of PIK3R1/P50 and directly enhance its transcription. We also identified p300/CBP as a transcriptional coregulator of CapG, which is recruited to PIK3R1 promoter through interaction with CapG, thereby increasing PIK3R1/P50 transcription by enhancing histone H3K27 acetylation. Consistently, inhibiting p300/CBP substantially decreased CapG-dependent upregulation of PIK3R1/P50 and subsequent PI3K/Akt activation, resulting in increased sensitivity to paclitaxel treatment in breast cancer cells. Conclusion: High CapG levels may predict poor paclitaxel response in breast cancer patients. Targeting CapG-mediated hyperactivation of PI3K/Akt pathway may mitigate resistance to chemotherapy in breast cancer.

19.
ACS Omega ; 4(17): 17547-17555, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31656928

RESUMO

The exploitation of natural gas hydrate is always hindered by the migration of water and sands due to gas production. Depressurization combined with thermal stimulation is an effective method for hydrate dissociation. This paper reported the influence of gas-liquid-solid migration on morphological change of hydrate sediments in natural gas production using visualization method. Different backpressures combined with thermal stimulation methods were applied to simulate natural gas hydrate exploitation. Pressure compensation was first employed to study sediment recovery features. The expansion rate of a porous medium layer under combined dissociation and different backpressure (4.5, 3.5, 2.5, 1.5, and 0.1 MPa) was discussed. A 176% hydrate sediment expansion rate was found after the combined dissociation at 0.1 MPa. In addition, it was observed that the height of the water layer above the porous media after pressure compensation was gradually reduced with a decrease in backpressure and eventually disappeared at 0.1 MPa. It was also found that the disappearing water layer caused an anomalous memory effect phenomenon. Expansion and subsidence of sediments provide a better reference for hydrate exploitation and geological safety.

20.
Bioinformatics ; 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31617571

RESUMO

MOTIVATION: Electron tomography (ET) offers a unique capacity to image biological structures in situ. However, the resolution of ET reconstructed tomograms is not comparable to that of the single-particle cryo-EM. If many copies of the object of interest are present in the tomograms, their structures can be reconstructed in the tomogram, picked, aligned and averaged to increase the signal-to-noise ratio and improve the resolution, which is known as the subtomogram averaging (STA). To date, the resolution improvement of the subtomogram averaging is still limited because each reconstructed subtomogram is of low reconstruction quality due to the missing wedge issue.% issue in the tilt series images. RESULTS: In this paper, we propose a novel computational model, the constrained reconstruction model (CRM), to better recover the information from the multiple subtomograms and compensate for the missing wedge issue in each of them. CRM is supposed to produce a refined reconstruction in the final turn of subtomogram averaging after alignment, instead of directly taking the average. We first formulate the averaging method and our CRM as linear systems, and prove that the solution space of CRM is no larger, and in practice much smaller, than that of the averaging method. We then propose a sparse Kaczmarz algorithm to solve the formulated CRM, and further extend the solution to the simultaneous algebraic reconstruction technique (SART). Experimental results demonstrate that CRM can significantly alleviate the missing wedge issue and improve the final reconstruction quality. In addition, our model is robust to the number of images in each tilt series, the tilt range, and the noise level. AVAILABILITY: The codes of CRM-SIRT and CRM-SART are available at https://github.com/icthrm/CRM.

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