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1.
Inorg Chem ; 61(21): 8153-8159, 2022 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-35580155

RESUMO

Donor-acceptor (D-A) hybrid frameworks with visual X-ray photochromism at room temperature are fascinating because of their promising applications as X-ray detectors. Herein, a 3-fold interpenetrated D-A hybrid framework, [Eu(bcbp)1.5(DMF)(H2O)2][Co(CN)6]·4H2O·CH3OH (1), has been obtained by incorporating electron-rich Co(CN)63- into the electron-deficient europium viologen framework, which interestingly exhibits ultraviolet and low-power X-ray dual photochromism with a remarkable color change from brown to green. Experimental and theoretical studies revealed that the X-ray photochromic behavior of hybrid 1 could be attributed to its D-A hybrid structural feature increasing the extent of photoinduced electron transfer and thus photogenerated radical species upon X-ray irradiation. Meanwhile, due to the introduction of emissive lanthanide cations in the D-A system, hybrid 1 exhibits photomodulated luminescence properties.

2.
Small ; 18(26): e2201159, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35589558

RESUMO

Just as the heterojunctions in physics, donor-acceptor (D-A) heterostructures are an emerging class of photoactive materials fabricated from two semiconductive components at the molecular level. Among them, D-A hybrid heterostructures from organic and inorganic semiconductive components have attracted extensive attention in the past decades due to their combined advantages of high stability for the inorganic semiconductors and modifiability for the organic semiconductors, which are particularly beneficial to efficiently achieve photoinduced charge separation and transfer upon irradiations. In this review, by analogy with the heterojunctions in physics, a definition of the D-A heterostructures and their general design and synthetic strategies are given. Meanwhile, the D-A hybrid heterostructures are focused on and their recent advances in potential applications of photochromism, photomodulated luminescence, and photocatalysis summarized.

3.
Inorg Chem ; 61(1): 105-112, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34918511

RESUMO

The self-assembly of electron-deficient protonated N, N'-dipyridyltetrachloroperylenediimide (4Cl-DPPDI) and electron-rich polyoxometalate acids HnXM12O40 (POMs; X = P or Si; M = W or Mo) resulted in four isomorphous donor-acceptor hybrid crystals 1-4 with segregated POM anions and one-dimensional racemic hydrogen-bonded 4Cl-DPPDI networks as electron-donor and -acceptor components, respectively. Because of the compact contacts between the POM anions and 4Cl-DPPDI tectons induced by anion-π interactions, besides enhanced photochromism, these four unique isostructural hybrids exhibited unusual room-temperature phosphorescence (RTP) emissions. More interestingly, owing to the facial compact contacts of two racemic 4Cl-DPPDI tectons induced by lone pair-π-assisted π-π interactions, they also showed unprecedented photon upconversion by triplet-triplet annihilation (TTA).

4.
Mol Ecol ; 30(23): 6273-6288, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34845798

RESUMO

Whole-genome sequencing has advanced the study of species evolution, including the detection of genealogical discordant events such as ancient hybridization and incomplete lineage sorting (ILS). The evolutionary history of bighorn (Ovis canadensis) and thinhorn (Ovis dalli) sheep present an ideal system to investigate evolutionary discordance due to their recent and rapid radiation and putative secondary contact between bighorn and thinhorn sheep subspecies, specifically the dark pelage Stone sheep (O. dalli stonei) and predominately white Dall sheep (O. dalli dalli), during the last ice age. Here, we used multiple genomes of bighorn and thinhorn sheep, together with snow (O. nivicola) and the domestic sheep (O. aries) as outgroups, to assess their phylogenomic history, potential introgression patterns and their adaptive consequences. Among the Pachyceriforms (snow, bighorn and thinhorn sheep) a consistent monophyletic species tree was retrieved; however, many genealogical discordance patterns were observed. Alternative phylogenies frequently placed Stone and bighorn as sister clades. This relationship occurred more often and was less divergent than that between Dall and bighorn. We also observed many blocks containing introgression signal between Stone and bighorn genomes in which coat colour genes were present. Introgression signals observed between Dall and bighorn were more random and less frequent, and therefore probably due to ILS or intermediary secondary contact. These results strongly suggest that Stone sheep originated from a complex series of events, characterized by multiple, ancient periods of secondary contact with bighorn sheep.


Assuntos
Doenças dos Ovinos , Carneiro da Montanha , Animais , Genoma , Hibridização Genética , Filogenia , Ovinos/genética , Carneiro da Montanha/genética
5.
Commun Biol ; 4(1): 1307, 2021 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-34795381

RESUMO

The domestication and subsequent development of sheep are crucial events in the history of human civilization and the agricultural revolution. However, the impact of interspecific introgression on the genomic regions under domestication and subsequent selection remains unclear. Here, we analyze the whole genomes of domestic sheep and their wild relative species. We found introgression from wild sheep such as the snow sheep and its American relatives (bighorn and thinhorn sheep) into urial, Asiatic and European mouflons. We observed independent events of adaptive introgression from wild sheep into the Asiatic and European mouflons, as well as shared introgressed regions from both snow sheep and argali into Asiatic mouflon before or during the domestication process. We revealed European mouflons might arise through hybridization events between a now extinct sheep in Europe and feral domesticated sheep around 6000-5000 years BP. We also unveiled later introgressions from wild sheep to their sympatric domestic sheep after domestication. Several of the introgression events contain loci with candidate domestication genes (e.g., PAPPA2, NR6A1, SH3GL3, RFX3 and CAMK4), associated with morphological, immune, reproduction or production traits (wool/meat/milk). We also detected introgression events that introduced genes related to nervous response (NEURL1), neurogenesis (PRUNE2), hearing ability (USH2A), and placental viability (PAG11 and PAG3) into domestic sheep and their ancestral wild species from other wild species.


Assuntos
Domesticação , Fluxo Gênico , Filogenia , Seleção Genética , Ovinos/genética , Animais , Carneiro da Montanha/genética , Carneiro Doméstico/genética , Sequenciamento Completo do Genoma
6.
Inorg Chem ; 60(21): 16233-16240, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34648276

RESUMO

Donor-acceptor (D-A) hybrid crystals are an emerging kind of crystalline hybrid material composed of semiconductive inorganic donors and organic acceptors. Except for the intrinsic photochromism, recently we have reported that the anion-π polyoxometalate (POM)/naphthalenediimide (NDI) hybrid crystals could produce an interesting room temperature phosphorescence (RTP) quantum yield up to 7.2%. Herein, we extended into core-substituted NDIs and anticipated the regulation of their photochromic and RTP properties. Thus, two hybrid crystals, namely (H4BDMPy-Br2NDI)·(NMP)4·(HPW12O40) (1) and (H4BDMPy-I2NDI)·(HPW12O40) (2) (H2BDMPy-Br2NDI: N,N'-bis(3,5-dimethylpyrazolyl)-2,6-dibromo-1,4,5,8-naphthalenediimide and H2BDMPy-I2NDI: N,N'-bis(3,5-dimethylpyrazolyl)-2,6-diiodide-1,4,5,8-naphthalenediimide), have been synthesized from phosphotungstic anions (PW12O403-) and Br or I core-substituted NDIs. Compared to the core-unsubstituted analogues (H4BDMPy-NDI)·(NMP)4·(HPW12O40) (3), 2 with photosensitive iodine substituents is more sensitive to light, which can become discolored under natural light. As a result of the heavy-atom effect, hybrid 1 exhibits remarkable RTP with the quantum yield up to 10.2% and a lifetime of 1.14 ms.

7.
Macromol Rapid Commun ; 42(24): e2100577, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34626510

RESUMO

Photocatalysis is an efficient and green technology in the environmental protection. Due to the high charge separation and transfer, donor-acceptor (D-A) conjugated polymers attract much attention for their photocatalytic degradations towards organic pollutants. Herein, the authors reported three novel D-A conjugated polymers, named as HPBP, HPTP, and HPF, with heptazine moieties as electron acceptors, while biphenyl, terphenyl, or fluorene moieties as electron donors, respectively, which indeed exhibit a highly efficient photocatalytic degradation towards tetracyclines upon the visible-light irradiation. Among them, the photocatalytic performance of HPF is especially noticeable with the degradation rate up to 87% within 30 min, almost 11 times in comparison to those of pristine g-C3 N4 , which is mainly attributed to its high crystallinity and conjugation. For their photocatalytic mechanism, the •O2 - radical anions are regarded as the active species.


Assuntos
Polímeros , Tetraciclinas , Compostos Heterocíclicos com 3 Anéis , Triazinas
8.
Dalton Trans ; 50(39): 13961-13967, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34533148

RESUMO

D-A hybrid heterostructures are an emerging class of crystalline hybrid materials composed of semiconductive inorganic donors and organic acceptors. However, due to the steric effects of the inorganic coordination sites, it is difficult for the large organic molecules to form compact packing at the molecular level, resulting in the poor efficiency of photoinduced charge transfers. To achieve an effective carrier separation and transfer, herein we incorporated third donors into a copper(I) halide/thiazolo[5,4-d] thiazole D-A heterostructure to construct three novel three-component complexes (Me2-Py2TTz)Cu4I6·(I2) (1), (Me2-Py2TTz)Cu3I5·(pyrene) (2) and (Me2-Py2TTz)Cu3I5·(perylene) (3) (Py2TTz = 2,5-bis(4-pyridyl) thiazolo[5,4-d] thiazole), respectively. Due to the spatial distances as well as the orbital energies between the copper(I) halide and thiazolo[5,4-d] thiazole units bridged by third donors, they are excellent three-component charge-transfer complexes (CTCs) with enhanced electrical properties.

10.
Front Genet ; 12: 670582, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34093663

RESUMO

Copy number variations (CNVs) are a major source of structural variation in mammalian genomes. Here, we characterized the genome-wide CNV in 2059 sheep from 67 populations all over the world using the Ovine Infinium HD (600K) SNP BeadChip. We tested their associations with distinct phenotypic traits by conducting multiple independent genome-wide tests. In total, we detected 7547 unique CNVs and 18,152 CNV events in 1217 non-redundant CNV regions (CNVRs), covering 245 Mb (∼10%) of the whole sheep genome. We identified seven CNVRs with frequencies correlating to geographical origins and 107 CNVRs overlapping 53 known quantitative trait loci (QTLs). Gene ontology and pathway enrichment analyses of CNV-overlapping genes revealed their common involvement in energy metabolism, endocrine regulation, nervous system development, cell proliferation, immune, and reproduction. For the phenotypic traits, we detected significantly associated (adjusted P < 0.05) CNVRs harboring functional candidate genes, such as SBNO2 for polycerate; PPP1R11 and GABBR1 for tail weight; AKT1 for supernumerary nipple; CSRP1, WNT7B, HMX1, and FGFR3 for ear size; and NOS3 and FILIP1 in Wadi sheep; SNRPD3, KHDRBS2, and SDCCAG3 in Hu sheep; NOS3, BMP1, and SLC19A1 in Icelandic; CDK2 in Finnsheep; MICA in Romanov; and REEP4 in Texel sheep for litter size. These CNVs and associated genes are important markers for molecular breeding of sheep and other livestock species.

11.
Life (Basel) ; 11(3)2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33803851

RESUMO

Currently, the intraspecific taxonomy of snow sheep (Ovis nivicola) is controversial and needs to be specified using DNA molecular genetic markers. In our previous work using whole-genome single nucleotide polymorphism (SNP) analysis, we found that the population inhabiting Kharaulakh Ridge was genetically different from the other populations of Yakut subspecies to which it was usually referred. Here, our study was aimed at the clarification of taxonomic status of Kharaulakh snow sheep using mitochondrial cytochrome b gene. A total of 87 specimens from five different geographic locations of Yakut snow sheep as well as 20 specimens of other recognized subspecies were included in this study. We identified 19 haplotypes, two of which belonged to the population from Kharaulakh Ridge. Median-joining network and Bayesian tree analyses revealed that Kharaulakh population clustered separately from all the other Yakut snow sheep. The divergence time between Kharaulakh population and Yakut snow sheep was estimated as 0.48 ± 0.19 MYA. Thus, the study of the mtDNA cytb sequences confirmed the results of genome-wide SNP analysis. Taking into account the high degree of divergence of Kharaulakh snow sheep from other groups, identified by both nuclear and mitochondrial DNA markers, we propose to classify the Kharaulakh population as a separate subspecies.

12.
Dalton Trans ; 50(14): 4959-4966, 2021 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33877194

RESUMO

The incorporation of photochromic moieties into coordination polymers is of particular interest because it can endow them with various switching functions such as electrical conductivity, luminescence, and magnetism. In this context, a viologen ligand as a photochromic moiety was incorporated into 3d-4f heterobimetallic hexacyanoferrates, resulting in three novel 3-D photochromic complexes with different metal cations, namely {[Ln(BCEbpy) M(CN)6 (H2O)4]·2H2O}n (denoted as CoDy, CoEu, and FeDy, Ln = Dy, Eu; M = Fe, Co, H2BCEbpy·2Br = N,N'-bis(carboxymethyl)-4,4'-bipyridinium dibromide). And the photoresponsive mechanism has been well discussed based on the solid UV-vis, IR, ESR, photoluminescence, and magnetism data. Moreover, accompanying the photochromic process, these unique complexes exhibit different photomagnetic behaviors upon UV-vis irradiation at RT because of the different ferromagnetic coupling interactions between photogenerated radicals and lanthanide cations.

13.
Mol Biol Evol ; 38(3): 838-855, 2021 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-32941615

RESUMO

How animals, particularly livestock, adapt to various climates and environments over short evolutionary time is of fundamental biological interest. Further, understanding the genetic mechanisms of adaptation in indigenous livestock populations is important for designing appropriate breeding programs to cope with the impacts of changing climate. Here, we conducted a comprehensive genomic analysis of diversity, interspecies introgression, and climate-mediated selective signatures in a global sample of sheep and their wild relatives. By examining 600K and 50K genome-wide single nucleotide polymorphism data from 3,447 samples representing 111 domestic sheep populations and 403 samples from all their seven wild relatives (argali, Asiatic mouflon, European mouflon, urial, snow sheep, bighorn, and thinhorn sheep), coupled with 88 whole-genome sequences, we detected clear signals of common introgression from wild relatives into sympatric domestic populations, thereby increasing their genomic diversities. The introgressions provided beneficial genetic variants in native populations, which were significantly associated with local climatic adaptation. We observed common introgression signals of alleles in olfactory-related genes (e.g., ADCY3 and TRPV1) and the PADI gene family including in particular PADI2, which is associated with antibacterial innate immunity. Further analyses of whole-genome sequences showed that the introgressed alleles in a specific region of PADI2 (chr2: 248,302,667-248,306,614) correlate with resistance to pneumonia. We conclude that wild introgression enhanced climatic adaptation and resistance to pneumonia in sheep. This has enabled them to adapt to varying climatic and environmental conditions after domestication.


Assuntos
Adaptação Biológica/genética , Resistência à Doença/genética , Introgressão Genética , Ovinos/genética , Animais , Evolução Biológica , Mudança Climática , Variação Genética , Filogeografia , Pneumonia/imunologia , Ovinos/imunologia
14.
Dalton Trans ; 49(37): 13083-13089, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-32929431

RESUMO

As an emerging class of hybrid complexes, donor-acceptor (D-A) hybrid heterostructures with advantages of both photoactive organic and inorganic components have provided an excellent platform for the fabrication of multifunctional photoactive materials. In this context, we have demonstrated three novel host-guest D-A hybrid heterostructures, {[Ln(BCEbpy)(H2O)4][CoIII(CN)6]·4H2O}n (1 (Eu), 2 (Dy), 3 (Sm)), based on the anionic Co(CN)63- and cationic coordination layers assembled from a viologen functionalized tecton and Ln(NO)3. Due to the introduction of an electron donor, CoIII(CN)63-, the unique hybrid exhibits a highly sensitive and reversible photochromic transformation from light-yellow to brown upon UV-Vis irradiation. More interestingly, accompanied with this photochromic process, hybrid 1 simultaneously possesses a photomodulated fluorescence behaviour. In addition, hybrid 1 shows high sensitivity and selectivity towards Cr2O72- anions with a fairly small LOD of ca. 9.6 × 10-6 M.

15.
Curr Biol ; 30(20): 4085-4095.e6, 2020 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-32822607

RESUMO

The domestication and subsequent global dispersal of livestock are crucial events in human history, but the migratory episodes during the history of livestock remain poorly documented [1-3]. Here, we first developed a set of 493 novel ovine SNPs of the male-specific region of Y chromosome (MSY) by genome mapping. We then conducted a comprehensive genomic analysis of Y chromosome, mitochondrial DNA, and whole-genome sequence variations in a large number of 595 rams representing 118 domestic populations across the world. We detected four different paternal lineages of domestic sheep and resolved, at the global level, their paternal origins and differentiation. In Northern European breeds, several of which have retained primitive traits (e.g., a small body size and short or thin tails), and fat-tailed sheep, we found an overrepresentation of MSY lineages y-HC and y-HB, respectively. Using an approximate Bayesian computation approach, we reconstruct the demographic expansions associated with the segregation of primitive and fat-tailed phenotypes. These results together with archaeological evidence and historical data suggested the first expansion of early domestic hair sheep and the later expansion of fat-tailed sheep occurred ∼11,800-9,000 years BP and ∼5,300-1,700 years BP, respectively. These findings provide important insights into the history of migration and pastoralism of sheep across the Old World, which was associated with different breeding goals during the Neolithic agricultural revolution.


Assuntos
DNA Mitocondrial/genética , Genoma/genética , Polimorfismo de Nucleotídeo Único/genética , Carneiro Doméstico/genética , Cromossomo Y/genética , Animais , Cruzamento , Linhagem da Célula/genética , Mapeamento Cromossômico , Variação Genética/genética , Masculino , Mitocôndrias/genética , Fenótipo , Filogenia , Ovinos , Carneiro Doméstico/classificação , Sequenciamento Completo do Genoma
16.
Dalton Trans ; 49(35): 12411-12417, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32852023

RESUMO

D-A hybrid heterostructures are an attractive class of hybrid complexes composed of semiconducting organic and inorganic components, which make them potential candidates for applications in the photoelectric fields, particularly as photochromic materials. Herein, we report that the combination of metal cations (M = Zn2+ or Cd2+), silicomolybdic anions, and N,N-di(4-pyridyl)-1,4,5,8-naphthalene diimide (DPNDI) via two diffusion methods (A and B) led to four three-component D-A hybrid heterostructures with silicomolybdic anions as electron donors, and one-dimensional (1-D) naphthalenediimide coordination networks of different metal cations, [Zn2(DPNDI)2(H2O)4]·(SiMo12O40) (1-A and 1-B) and [Cd2(DPNDI)2(H2O)4]·(SiMo12O40) (2-A and 2-B), as electron acceptors. Although the different diffusion methods, 1-B, 2-A and 2-B, are isostructures with close cell parameters. Due to the different ionic radii and electronegativity of metal cations in isostructural 1-B and 2-B, they exhibit different electron-transfer photochromic behaviors. This study paves a new path for designing novel photochromic materials through such third-component metal cations.

17.
Anim Genet ; 51(5): 833-836, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32794198

RESUMO

MicroRNA resources in sheep are limited compared with those in other domesticated mammalian species. By sequencing small RNAs of sheep corpus luteum and endometrium, we have generated the largest amount of miRNA-seq data and compiled the most comprehensive list thus far of miRNAs (n = 599) in sheep. Additionally, we observed a highly conserved maternally imprinted cluster of miRNAs on chromosome 18 homologous to that found on chromosome 14 in human and several other eutherian mammals.


Assuntos
MicroRNAs/genética , Prenhez/genética , Carneiro Doméstico/genética , Animais , Feminino , Gravidez , Análise de Sequência de RNA/veterinária
18.
Nat Commun ; 11(1): 2815, 2020 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-32499537

RESUMO

Understanding the genetic changes underlying phenotypic variation in sheep (Ovis aries) may facilitate our efforts towards further improvement. Here, we report the deep resequencing of 248 sheep including the wild ancestor (O. orientalis), landraces, and improved breeds. We explored the sheep variome and selection signatures. We detected genomic regions harboring genes associated with distinct morphological and agronomic traits, which may be past and potential future targets of domestication, breeding, and selection. Furthermore, we found non-synonymous mutations in a set of plausible candidate genes and significant differences in their allele frequency distributions across breeds. We identified PDGFD as a likely causal gene for fat deposition in the tails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses. Our results provide insights into the demographic history of sheep and a valuable genomic resource for future genetic studies and improved genome-assisted breeding of sheep and other domestic animals.


Assuntos
Criação de Animais Domésticos/métodos , Animais Selvagens/genética , Fator de Crescimento Derivado de Plaquetas/metabolismo , Carneiro Doméstico/genética , Alelos , Animais , Cruzamento , Feminino , Frequência do Gene , Variação Genética , Genética , Genômica , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Desequilíbrio de Ligação , Mutação , Fenótipo , Polimorfismo de Nucleotídeo Único , Seleção Genética , Análise de Sequência de DNA , Ovinos , Especificidade da Espécie , Sequenciamento Completo do Genoma
19.
Genet Sel Evol ; 52(1): 25, 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32408891

RESUMO

BACKGROUND: In the Neolithic, domestic sheep migrated into Europe and subsequently spread in westerly and northwesterly directions. Reconstruction of these migrations and subsequent genetic events requires a more detailed characterization of the current phylogeographic differentiation. RESULTS: We collected 50 K single nucleotide polymorphism (SNP) profiles of Balkan sheep that are currently found near the major Neolithic point of entry into Europe, and combined these data with published genotypes from southwest-Asian, Mediterranean, central-European and north-European sheep and from Asian and European mouflons. We detected clines, ancestral components and admixture by using variants of common analysis tools: geography-informative supervised principal component analysis (PCA), breed-specific admixture analysis, across-breed [Formula: see text] profiles and phylogenetic analysis of regional pools of breeds. The regional Balkan sheep populations exhibit considerable genetic overlap, but are clearly distinct from the breeds in surrounding regions. The Asian mouflon did not influence the differentiation of the European domestic sheep and is only distantly related to present-day sheep, including those from Iran where the mouflons were sampled. We demonstrate the occurrence, from southeast to northwest Europe, of a continuously increasing ancestral component of up to 20% contributed by the European mouflon, which is assumed to descend from the original Neolithic domesticates. The overall patterns indicate that the Balkan region and Italy served as post-domestication migration hubs, from which wool sheep reached Spain and north Italy with subsequent migrations northwards. The documented dispersal of Tarentine wool sheep during the Roman period may have been part of this process. Our results also reproduce the documented 18th century admixture of Spanish Merino sheep into several central-European breeds. CONCLUSIONS: Our results contribute to a better understanding of the events that have created the present diversity pattern, which is relevant for the management of the genetic resources represented by the European sheep population.


Assuntos
Genética Populacional/métodos , Polimorfismo de Nucleotídeo Único/genética , Ovinos/genética , Animais , Península Balcânica , Cruzamento/métodos , Domesticação , Testes Genéticos/métodos , Variação Genética/genética , Genótipo , Filogenia , Filogeografia/métodos
20.
Genes (Basel) ; 11(4)2020 04 10.
Artigo em Inglês | MEDLINE | ID: mdl-32290341

RESUMO

The majority of pregnancy loss in ruminants occurs during the preimplantation stage, which is thus the most critical period determining reproductive success. Here, we performed a comparative transcriptome study by sequencing total mRNA from corpus luteum (CL) collected during the preimplantation stage of pregnancy in Finnsheep, Texel and F1 crosses. A total of 21,287 genes were expressed in our data. Highly expressed autosomal genes in the CL were associated with biological processes such as progesterone formation (STAR, CYP11A1, and HSD3B1) and embryo implantation (e.g., TIMP1, TIMP2 and TCTP). Among the list of differentially expressed genes, sialic acid-binding immunoglobulin (Ig)-like lectins (SIGLEC3, SIGLEC14, SIGLEC8), ribosomal proteins (RPL17, RPL34, RPS3A, MRPS33) and chemokines (CCL5, CCL24, CXCL13, CXCL9) were upregulated in Finnsheep, while four multidrug resistance-associated proteins (MRPs) were upregulated in Texel ewes. A total of 17 known genes and two uncharacterized non-coding RNAs (ncRNAs) were differentially expressed in breed-wise comparisons owing to the flushing diet effect. The significantly upregulated TXNL1 gene indicated potential for embryonic diapause in Finnsheep and F1. Moreover, we report, for the first time in any species, several genes that are active in the CL during early pregnancy (including TXNL1, SIGLEC14, SIGLEC8, MRP4, and CA5A).


Assuntos
Corpo Lúteo/citologia , Implantação do Embrião/genética , Perfilação da Expressão Gênica , Reprodução , Ovinos/crescimento & desenvolvimento , Ovinos/genética , Transcriptoma , Animais , Corpo Lúteo/metabolismo , Feminino , Gravidez
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