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2.
Carbohydr Polym ; 264: 118033, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33910743

RESUMO

Attributed to low cost, renewable, and high availability, cellulose-based aerogels are desirable materials for various applications. However, mechanical robustness and functionalization remain huge challenges. Herein, we synthesized a recoverable, anisotropic cellulose nanofiber (CNF) / chitosan (CS) aerogel via directional freeze casting and chemical cross-link process. The chitosan was performed as strength polymers to prohibits the shrinkage and retains the structural stability of 3D cellulose nanofiber skeleton, endowing the composite aerogel with satisfactory deformation recovery ability (without loss under 60 % stress cycled 100 times). The CNF/CS composite aerogel has ultralow density (∼8.4 mg/cm3), high temperature-invariant (above 300 °C) and high porosity (98 %). The CNF/CS aerogel demonstrates anisotropic thermal insulation properties with low thermal conductivity (28 mWm-1 K-1 in rational direction and 36 mW m-1 K-1 in the axial direction). Moreover, the composite aerogel (water contact angle ∼148°) exhibited outstanding oil/water selectivity and high absorption capacity (82-253 g/g) for various oils and organic solvents. Therefore, the multifunctional CNF/CS composite aerogels are potential materials for thermal management and oil absorption applications.

3.
ACS Appl Mater Interfaces ; 13(15): 18033-18046, 2021 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-33834754

RESUMO

Natural killer (NK) cell-based immunotherapy presents a promising antitumor strategy and holds potential for combination with chemotherapy. However, the suppressed NK cell activity and poor tumor retention of therapeutics hinder the efficacy. To activate NK cell-based immuno-chemotherapy and enhance the tumor retention, we proposed a pH-responsive self-aggregated nanoparticle for the codelivery of chemotherapeutic doxorubicin (DOX) and the transforming growth factor-ß (TGF-ß)/Smad3 signaling pathway inhibitor SIS3. Polycaprolactone-poly(ethylene glycol) (PCL-PEG2000) micelles modified with dibenzylcyclooctyne (DBCO) or azido (N3) and coated with acid-cleavable PEG5000 were established. This nanoplatform, namely, M-DN@DOX/SIS3, could remain well dispersed in the neutral systemic circulation, while quickly respond to the acidic tumor microenvironment and intracellular lysosomes, triggering copper-free click reaction-mediated aggregation, leading to the increased tumor accumulation and reduced cellular efflux. In addition, the combination of DOX with SIS3 facilitated by the aggregation strategy resulted in potent inhibition of melanoma tumor growth and significantly increased NK cells, NK cell cytokines, and antitumor T cells in the tumor. Taken together, our study offered a new concept of applying copper-free click chemistry to achieve nanoparticle aggregation and enhance tumor retention, as well as a promising new combined tumor treatment approach of chemotherapy and immunotherapy.

4.
Int J Biol Sci ; 17(5): 1234-1249, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33867842

RESUMO

Dickkopf-1 (DKK1) was recently shown to play an important role in cardiovascular disease. The aim of this work was to assess the role of DKK1 in the regulation of smooth muscle cell function by mechanical stretch and the mechanisms underlying this process. Methods: Wild-type C57BL/6J mice were subjected to sham or abdominal aortic constriction (AAC) surgery. The expression level of DKK1 was examined by immunohistochemical staining and Western blotting. Analyses of DKK1 function in vascular smooth muscle cell (VSMC) proliferation and migration were performed. Transcriptome sequencing analysis was performed to identify the differentially expressed genes and pathways regulated by DKK1. Smooth muscle-specific Dkk1 knockout mice were used to confirm the function of DKK1 in vivo. Chromatin immunoprecipitation (ChIP) was used to confirm DNA-protein interactions. Promoter luciferase analysis was used to detect transcription factor activity. Results: We found that AAC significantly increased DKK1 protein levels in the thoracic aorta and coronary artery in vivo. In vitro, high-level stretch (18%) induced the expression of DKK1 in VSMCs. Knocking down DKK1 inhibited VSMC proliferation and migration under high-level stretch (18%). We identified ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) as a target gene of DKK1. Knockdown of UHRF1 with small interfering RNAs partially reversed the regulatory effect of recombinant DKK1 on VSMCs. Specific deletion of DKK1 in VSMCs was sufficient to attenuate the AAC-induced upregulation of UHRF1, thickening of arterial media and increase in VSMC proliferation. Furthermore, we found that DKK1 regulated UHRF1 expression through the YAP-TEAD pathway. TEAD1 and TEAD4 bound directly to the promoter of UHRF1, and blocking the YAP-TEAD interaction inhibited UHRF1 upregulation due to DKK1. Conclusions: This study reveals that DKK1 mediates the mechanical stretch regulation of smooth muscle cell function by modulating UHRF1 expression through the YAP-TEAD pathway.

5.
Behav Brain Res ; 409: 113289, 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33836168

RESUMO

How to reach the goal is one of the core problems that animals must solve to complete goal-directed behavior. Studies have proved the important role of hippocampus (Hp) in spatial navigation and shown that hippocampal neural activities can represent the current location and goal location. However, for the different routes linking these two locations, the neural representation mechanism of the route selection in Hp is not clear. Here, we addressed this question using neural recordings of Hp ensembles and decoding analyses in pigeons performing a goal-directed route selection task known to require Hp participation. The hippocampal spike trains and local field potentials (LFPs) of five pigeons performing the task were acquired and analyzed. We found that the neuron firing rates and power spectrum characteristics in Hp could encode the animal's route selection during goal-directed behavior, suggesting that the representation of route selection was coherent for hippocampal spike and LFP signals. Decoding results further indicated that joint spike-LFP features resulted in a significant improvement in the representation accuracy of the route selection. These findings of this study will help to understand the encoding mechanism of route selection in goal-directed behavior.

6.
BMC Pulm Med ; 21(1): 111, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33794845

RESUMO

BACKGROUND: SMAD4 has been found to be inactivated to varying degrees in many types of cancer; the purpose of this study was to investigate the correlation between SMAD4 expression in non-small cell lung cancer (NSCLC) and clinical pathological parameters. METHODS: The serum concentration of SMAD4 was measured by enzyme-linked immunosorbent assay and its histological expression was quantified by immunohistochemistry. RESULTS: The serum concentration of Smad4 in patients with NSCLC was lower than that in benign lung disease patients and healthy individuals (P < 0.001) and its concentration was related to the histological classification, pathological differentiation, lymphatic metastasis and clinical stage of NSCLC. The sensitivity and specificity of serum Smad4 were 91.56% and 61.56% for screening NSCLC from healthy individuals and 84.55% and 60.36% for screening NSCLC from patients with benign lung disease. Logistic regression analysis showed that the degree of cell differentiation (P < 0.001), lymph node metastasis (P < 0.001) and clinical stage of NSCLC (P = 0.007) affected the expression of Smad4, and had a strong correlation with the expression of Smad4. The expression of Smad4 in NSCLC tissues was lower than that in normal lung tissues (P = 0.009) and its expression was related to the degree of tissue differentiation, lymph node metastasis and clinical stage (P < 0.05). CONCLUSIONS: The downregulation or deletion of Smad4 is related to the malignant biological behavior of NSCLC and serum Smad4 could be considered as a potential molecular indicator for diagnosis and evaluation of NSCLC.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33895906

RESUMO

Six aerobic Gram-negative bacteria were isolated from seawater in Guangdong Province, P.R. China. Cells were observed to be Gram-negative, aerobic, non-motile and non-spore forming. Growth of the designated type strain 19X3-30T occurred at a temperature range of 14-37 °C (optimum, 28 °C), a pH range of 6.0-8.0 (optimum, pH 7) and up to 7.5% NaCl (optimum, 1.5%; w/v), and was enhanced by CO2 and L-cysteine supplementation. The major polar lipids identified in strain 19X3-30T were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The principal cellular fatty acids profile showed the presence of anteiso-C15:0, anteiso-C17:0 and C18:0 (> 8% of total fatty acids), and the respiratory quinone was ubiquinone 8 (UQ-8). According to the analysis of 16S rRNA gene sequences, these strains represented a novel species within the family Fastidiosibacteraceae, sharing maximum similarities with Cysteiniphilum litorale DSM 101832T (96.6%) and Cysteiniphilum halobium DSM 103992T (95.3%). Phylogenetic dendrograms based on 16S rRNA gene and protein marker genes from the genomic sequences both indicated that the strains formed a monophyletic lineage closely linked to the genus Cysteiniphilum, which was also supported by the UPGMA dendrogram based on the MALDI-TOF MS profile. The genomic DNA G + C contents of six strains ranged from 38.0% to 38.1%. Based on different taxonomic genomic metrics, phylogeny and phenotypic features, we propose that the strains warrant the assignment to a novel species, for which the name Cysteiniphilum marinum sp. nov. is proposed. The type strain is 19X3-30T (= KCTC 82154T = CGMCC 1.18585T).

8.
Epilepsy Behav ; 117: 107866, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33684784

RESUMO

OBJECTIVE: This study aimed to investigate the factors influencing the level of social anxiety in patients with epilepsy (PWE) in Northeast China. We also identified the effect of social anxiety on the quality of life in these patients. METHODS: A consecutive cohort of 148 adult PWE from The First Hospital of Jilin University were recruited. In this sample, 116 patients had focal epilepsy, 20 had generalized epilepsy, and 12 had unclassified epilepsy. Depressive symptoms, social anxiety, and quality of life were evaluated using the Chinese version of the Patient Health Questionnaire 9 (PHQ-9), 20-item Social Phobia Scale (SPS), 20-item Social Interaction Anxiety Scale (SIAS), and Quality-of-Life Inventory in Epilepsy-31 (QOLIE-31), respectively. Multivariate linear regression analyses were employed to identify independent factors influencing SPS scores and SIAS scores. RESULTS: Correlation analysis suggested that sex, age at onset, seizure frequency over the last year, AED treatment model, >50% nocturnal seizures, PHQ-9 score, and QOLIE-31 score had a significant correlation with the SPS score. The age at onset, seizure frequency over the last year, AED treatment model, PHQ-9 score, and QOLIE-31 score correlated with the SIAS score. Multiple linear regression analysis showed that the total QOLIE-31 score (ß = - 0.481; p = 0.001) was inversely associated with the SPS score in PWE. Additionally, earlier age of onset (ß = -0.156; p = 0.022) and low total QOLIE-31 score (ß = -0.457; p = 0.001) were risk factors for high SIAS scores. CONCLUSION: We found that social anxiety was independently associated with poor quality of life. Earlier age of onset was also a risk factor for social anxiety. Future studies with large sample sizes are required to confirm our findings.


Assuntos
Epilepsia , Qualidade de Vida , Adulto , Ansiedade/epidemiologia , Ansiedade/etiologia , China/epidemiologia , Estudos Transversais , Epilepsia/complicações , Epilepsia/epidemiologia , Humanos , Inquéritos e Questionários
9.
Clin Neurol Neurosurg ; 203: 106542, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33706063

RESUMO

AIM: To examine the clinical characteristics, laboratory tests, imaging and electroencephalography presentation, treatment, and prognosis of anti-leucine-rich glioma-inactivated 1 (anti-LGI1) encephalitis and improve the awareness of this disease. METHOD: We performed a retrospective analysis of the clinical data of 41 patients who were diagnosed with anti-LGI1 encephalitis. Their clinical characteristics, laboratory tests, and imaging and electroencephalography data were collected, and the treatment results and prognosis were evaluated. The modified Rankin Scale (mRS) was used to evaluate neurological function. RESULTS: A total of 41 patients were included in the study, the average follow-up time is 33.0 months.The initial symptoms included cognitive impairment (n = 16, 39.0%),faciobrachial dystonic seizures (FBDS) (n = 12, 29.3%), grand mal seizures (n = 5, 12.2%) hallucinations (n = 4, 9.8%), loss of consciousness (n = 2, 4.9%), nausea and vomiting (n = 1, 2.4%),and head discharge-like sensation and radiate one limb (n = 1, 2.4%). There were 20 and 21 patients in the good (mRS ≤ 2) and poor (mRS > 2) prognosis groups, respectively. In the good prognosis group, the initial symptoms included faciobrachial dystonic seizures (FBDS) (n = 6, 30.0%), cognitive impairment (n = 6, 30.0%), hallucinations (n = 4, 20.0%), grand mal seizures (n = 3, 15.0%), loss of consciousness (n = 2, 10.0%), and nausea and vomiting (n = 1, 5.0%). There were 10 patients with hyponatremia. Magnetic resonance imaging (MRI) showed limbic system involvement in 12 patients (60.0%).17 patients (85.0%) recovered, 2 (10.0%) showed significant improvement, and 1 (5.0%) died after a mean follow-up period of 36.9 months. In the poor prognosis group, the initial symptoms included FBDS (n = 6, 28.5%), cognitive impairment (n = 10, 47.6%), grand mal seizures (n = 2, 9.5%), and electric shock-like sensation in the left limbs (n = 1, 4.7%). There were 20 patients with hyponatremia. MRI showed limbic system involvement in 11 patients (52.4%). 11 patients (52.4%) recovered, 8 (38.1%) showed significant improvement, and 2 (9.5%) died after a mean follow-up period of 29.0 months. CONCLUSIONS: The clinical characteristics of anti-LGI1 encephalitis include hyponatremia, FBDS, epileptic seizures, hallucinations, cognitive impairment, and loss of consciousness, while the rarely seen characteristics are nausea, vomiting, and other autonomic dysfunctions and electric shock-like sensation. The appearance of hallucinations often indicates a good prognosis.Hyponatremia and elevated cerebrospinal fluid protein levels can be used as indicators that affect the prognosis of patients.Limbic system involvement has nothing to do with prognosis.Attention should be paid to early diagnosis and timely first-line immunotherapy.

10.
Br J Nutr ; : 1-9, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33663633

RESUMO

N-3 long-chain (≥C20) PUFA (LC-PUFA) are vital fatty acids for fish and humans. As a main source of n-3 LC-PUFA for human consumers, the n-3 LC-PUFA content of farmed fish is important. Previously, we identified fatty acid-binding protein (fabp)-4 as a candidate gene for regulating the n-3 LC-PUFA content. Herein, we further assessed the role of fabp4 in this process. First, a 2059 bp promoter sequence of fabp4 in Trachinotus ovatus was cloned and, using progressive deletion, determined -2006 bp to -1521 bp to be the core promoter sequence. The PPAR-γ binding sites were predicted to occur in this region. A luciferase reporter assay showed that the promoter activity of fabp4 decreased following mutation of the PPARγ binding site and that PPARγ increased the fabp4 promoter activity in a dose-dependent manner, implying that T. ovatus fabp4 is a target of PPARγ. The overexpression of fabp4 or PPARγ increased the DHA content in hepatocytes, whereas suppression of their expression diminished this effect, suggesting that both fabp4 and PPARγ play an active role in regulating DHA content. Moreover, the inhibition of fabp4 attenuated the increase in PPARγ-mediated DHA content, and the overexpression of fabp4 alleviated this effect. Collectively, our findings indicated that fabp4, which is controlled by PPARγ, plays an important role in DHA content regulation. The new regulation axis can be considered a promising novel target for increasing the n-3 LC-PUFA content in T. ovatus.

11.
Phys Med Biol ; 66(4): 04NT01, 2021 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-33527911

RESUMO

The purpose of this work was to develop and evaluate a deep learning approach for automatic rat brain image segmentation of magnetic resonance imaging (MRI) images in a clinical PET/MR, providing a useful tool for analyzing studies of the pathology and progression of neurological disease and to validate new radiotracers and therapeutic agents. Rat brain PET/MR images (N = 56) were collected from a clinical PET/MR system using a dedicated small-animal imaging phased array coil. A segmentation method based on a triple cascaded convolutional neural network (CNN) was developed, where, for a rectangular region of interest covering the whole brain, the entire brain volume was outlined using a CNN, then the outlined brain was fed into the cascaded network to segment both the cerebellum and cerebrum, and finally the sub-cortical structures within the cerebrum including hippocampus, thalamus, striatum, lateral ventricles and prefrontal cortex were segmented out using the last cascaded CNN. The dice score coefficient (DSC) between manually drawn labels and predicted labels were used to quantitatively evaluate the segmentation accuracy. The proposed method achieved a mean DSC of 0.965, 0.927, 0.858, 0.594, 0.847, 0.674 and 0.838 for whole brain, cerebellum, hippocampus, lateral ventricles, striatum, prefrontal cortex and thalamus, respectively. Compared with the segmentation results reported in previous publications using atlas-based methods, the proposed method demonstrated improved performance in the whole brain and cerebellum segmentation. In conclusion, the proposed method achieved high accuracy for rat brain segmentation in MRI images from a clinical PET/MR and enabled the possibility of automatic rat brain image processing for small animal neurological research.

12.
J Mater Chem B ; 9(7): 1787-1791, 2021 02 25.
Artigo em Inglês | MEDLINE | ID: mdl-33595044

RESUMO

Iron-based coordination complexes are showing increasing potential to be alternatives for T1-weighted magnetic resonance imaging (MRI) and contribute to the safety of gadolinium-based compounds. In this work, three water-soluble iron-based complexes constructed using catechol ligands exhibiting T1-weighted MRI contrast behavior are described. The longitudinal relaxivity (r1) increase from 0.88 to 1.43 mM-1 s-1 mainly depends on the sizes and the number of water molecules in the second and outer spheres around the discrete complexes.

13.
Nat Commun ; 12(1): 1039, 2021 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-33589602

RESUMO

Recent studies have reported a variety of health consequences of climate change. However, the vulnerability of individuals and cities to climate change remains to be evaluated. We project the excess cause-, age-, region-, and education-specific mortality attributable to future high temperatures in 161 Chinese districts/counties using 28 global climate models (GCMs) under two representative concentration pathways (RCPs). To assess the influence of population ageing on the projection of future heat-related mortality, we further project the age-specific effect estimates under five shared socioeconomic pathways (SSPs). Heat-related excess mortality is projected to increase from 1.9% (95% eCI: 0.2-3.3%) in the 2010s to 2.4% (0.4-4.1%) in the 2030 s and 5.5% (0.5-9.9%) in the 2090 s under RCP8.5, with corresponding relative changes of 0.5% (0.0-1.2%) and 3.6% (-0.5-7.5%). The projected slopes are steeper in southern, eastern, central and northern China. People with cardiorespiratory diseases, females, the elderly and those with low educational attainment could be more affected. Population ageing amplifies future heat-related excess deaths 2.3- to 5.8-fold under different SSPs, particularly for the northeast region. Our findings can help guide public health responses to ameliorate the risk of climate change.


Assuntos
Doenças Cardiovasculares/mortalidade , Mudança Climática/mortalidade , Pneumopatias/mortalidade , Modelos Estatísticos , Saúde Pública/tendências , Adolescente , Adulto , Idoso , Doenças Cardiovasculares/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Simulação por Computador , Escolaridade , Feminino , Temperatura Alta , Humanos , Lactente , Recém-Nascido , Pneumopatias/epidemiologia , Masculino , Pessoa de Meia-Idade , Análise de Sobrevida
14.
Artigo em Inglês | MEDLINE | ID: mdl-33595283

RESUMO

High-performance aerogel fibers with high porosity, ultralow density and thermal conductivity, and good flexibility are attractive candidates for the next generation of effective thermal insulation, efficient personal thermal management, and other functional applications. However, most previously reported aerogel fibers suffered from either limited working temperatures, weak mechanical properties, or complex manufacturing processes. In the present work, a facile wet-spinning technique combined with freeze-drying was developed to fabricate strong polyimide aerogel fibers (PAFs) based on organo-soluble polyimide. Attributed to the unique "porous core-dense sheath" morphology, the PAFs exhibited excellent mechanical properties with an optimum tensile strength of 265 MPa and an initial modulus of 7.9 GPa at an ultimate elongation of 65%, representing the highest value for aerogel fibers reported so far. Moreover, the PAFs possess high porosity (>80%) and high specific surface area (464 m2 g-1), which render the woven PAF fabrics with excellent thermal insulation properties within a wide temperature range (-190 to 320 °C) and potential applications for thermal insulation under harsh environments. Additionally, a series of functionalized aerogel fibers or their fabrics based on PAFs, including phase-change fabrics with a thermoregulation function and electromagnetic shielding (EMI) textiles with a high EMI SE value, have been successfully fabricated for expanding their potential applications. Overall, this novel aerogel fiber sheds light on a promising direction for developing the next generation of high-performance thermal insulation and multifunctional fibers and textiles.

15.
Antonie Van Leeuwenhoek ; 114(4): 445-455, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33620611

RESUMO

Two bacterial strains SYSU G02173T and SYSU G03142 were isolated from hot springs in Tibet, China. Based on the results of nearly full-length 16S rRNA gene sequences and phylogenetic analyses, strains SYSU G02173T and SYSU G03142 were assigned to the family Sphingosinicellaceae, and were closest to Sandaracinobacter sibiricus RB16-17 T (96.04% and 96.12% similarity, respectively). Cells of the both new strains were observed to be motile rod-shape, Gram-staining negative. Growth occurred at pH 6-8 (optimal: pH 7.0) and 37-55 °C (optimal: 45 °C) with 0-1.0% (w/v) NaCl in T4 broth. The cells were found to be positive for oxidase and catalase activities. The major respiratory ubiquinone was Q-8. The major fatty acids were identified as summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, C14:0 2-OH. The major polar lipids were found to consist of sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, one unidentified glycolipid, three unidentified aminolipids and two unidentified polar lipids. The DNA G + C contents of strains SYSU G02173T and SYSU G03142 were 71.8%. The average nucleotide identity (ANI) value between strain SYSU G02173T and SYSU G03142 was 99.98%. The amino acid identity (AAI) values between them and their closely related species were below 66.14%. The isolates are characterized by aerobic growth, a yellow endocellular pigment and a higher optimum growth temperature. The results showed that strains SYSU G02173T and SYSU G03142 represent a novel species of a novel genus in the family Sphingomonadaceae, and thus the name Thermaurantiacus tibetensis (type strain SYSU G02173T = KCTC 72052 T = CGMCC 1.16680 T) is proposed.

16.
Theranostics ; 11(7): 3196-3212, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33537082

RESUMO

Resistance to chemotherapy is a long-standing problem in the management of cancer, and cancer stem cells are regarded as the main source of this resistance. This study aimed to investigate metallothionein (MT)-1G involvement in the regulation of cancer stemness and provide a strategy to overcome chemoresistance in pancreatic ductal adenocarcinoma (PDAC). Methods: MT1G was identified as a critical factor related with gemcitabine resistance in PDAC cells by mRNA microarray. Its effects on PDAC stemness were evaluated through sphere formation and tumorigenicity. LC-MS/MS analysis of conditional medium revealed that activin A, a NF-κB target, was a major protein secreted from gemcitabine resistant PDAC cells. Both loss-of-function and gain-of-function approaches were used to validate that MT1G inhibited NF-κB-activin A pathway. Orthotopic pancreatic tumor model was employed to explore the effects on gemcitabine resistance with recombinant follistatin to block activin A. Results: Downregulation of MT1G due to hypermethylation of its promoter is related with pancreatic cancer stemness. Secretome analysis revealed that activin A, a NF-κB target, was highly secreted by drug resistant cells. It promotes pancreatic cancer stemness in Smad4-dependent or independent manners. Mechanistically, MT1G negatively regulates NF-κB signaling and promotes the degradation of NF-κB p65 subunit by enhancing the expression of E3 ligase TRAF7. Blockade of activin A signaling with follistatin could overcome gemcitabine resistance. Conclusions: MT1G suppresses PDAC stemness by limiting activin A secretion via NF-κB inhibition. The blockade of the activin A signaling with follistatin may provide a promising therapeutic strategy for overcoming gemcitabine resistance in PDAC.

17.
J Neurol ; 2021 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-33616741

RESUMO

BACKGROUND: Helicobacter pylori (HP) infection has been reported to be associated with increased severity of Parkinson's disease (PD) and have negative effects on drug response in patients. We aimed to investigate the influence of HP infection on patients with PD using a systematic review and meta-analysis approach. METHODS: PubMed and EMBASE databases for relevant articles published before October 2020 were searched. Two authors independently screened records, extracted data, and evaluated the quality of the included studies. The odds ratios (ORs) or standardized mean differences (SMDs) with their corresponding 95% confidence intervals (CIs) were used to calculate the pooled results by employing a random or fixed-effects model. Sensitivity analyses were conducted, and potential publication bias was assessed. RESULTS: A total of 13 studies were included in our meta-analysis. Overall, PD patients with HP infection had significantly higher levodopa equivalent daily dose (UPDRS) motor scores (SMD = 0.266; 95% CI 0.065-0.467; P = 0.009) and more units of levodopa equivalent daily dose (LEDD) (SMD = 0.178; 95% CI 0.004-0.353; P = 0.046) than those of patients without HP infection. Additionally, the time to achieve 'ON' state was significantly longer (SMD = 0.778; 95% CI 0.337-1.220; P = 0.001) and the duration of 'ON' state was significantly shorter (SMD = -0.539; 95% CI = -0.801 to -0.227; P = 0.001) in patients with HP infection than in those without HP infection. CONCLUSION: Our pooled results of this meta-analysis demonstrated that HP infection was associated with worse motor symptoms, higher LEDD, and worse response to drugs in patients with PD. This evidence emphasizes the importance of considering subsequent eradication of HP infection in patients with PD.

18.
Epilepsy Behav ; 116: 107801, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33578225

RESUMO

OBJECTIVES: Evidence has indicated that there may be sex differences in the prevalence of and risk factors for anxiety in patients with epilepsy (PWE). The prevalence and risk factors for anxiety in male and female PWE in Northeast China were investigated. METHODS: A consecutive cohort of patients with epilepsy (PWE) from the First Hospital of Jilin University was recruited. Anxiety symptoms were assessed using the 7-item Generalized Anxiety Disorder-7 questionnaire (GAD-7; Chinese version). Multivariate logistic regression analyses were used to confirm independent risk factors for anxiety in male and female patients. RESULTS: Anxiety was prevalent in 28.2% (n = 162) of the total sample of patients, in 23.2% (n = 79) of males, and in 35.5% (n = 83) of females. Younger age (P = 0.033), higher seizure frequency over the last year (P = 0.003), and higher C-NDDI-E scores (P = 0.001) were risk factors for anxiety in males with epilepsy. Only higher C-NDDI-E scores (P = 0.001) had an independent effect on the risk of anxiety in females with epilepsy. CONCLUSION: Anxiety is a common psychiatric comorbidity among PWE. There were sex differences in the prevalence and risk factors for anxiety in patients.


Assuntos
Epilepsia , Caracteres Sexuais , Ansiedade/epidemiologia , Ansiedade/etiologia , Transtornos de Ansiedade , China/epidemiologia , Depressão , Epilepsia/complicações , Epilepsia/epidemiologia , Feminino , Humanos , Masculino , Fatores de Risco
19.
Biosens Bioelectron ; 176: 112934, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33418185

RESUMO

Near-infrared electrochemiluminescence (NIR ECL) luminophores are strongly anticipated in ECL bioimaging and spectrum-resolved multiplexing assays. Herein, bovine serum albumin-stabilized Ag nanoclusters (BSA-Ag NCs) are demonstrated promising NIR ECL performance, exhibiting strong anodic ECL spectrum peak at 904 nm in aqueous medium. The ECL intensity of BSA-Ag NCs-triethanolamine system was enhanced 3.2 times by adding TiO2 nanoparticles as co-reactant accelerator. In a spectrum-resolved triplex-color ECL multiplexing immunoassay, CdSe NCs, CdTe NCs and BSA-Ag NCs were used as the ECL tags, carbohydrate antigen 125 (CA125), carbohydrate antigen 19-9 (CA19-9) and cardiac troponin I (cTnI) were chosen as model analytes. The ECL peaks of three antigen-NCs pairs were simultaneously measured via one ECL potential scan. The composite of carbon nanotubes-TiO2 nanoparticles-chitosan was served as the conductivity and co-reactant accelerators, enhancing the ECL intensity by 16 folds. With the integral intensity over the ECL peaks as the analytical signal to improve further the signal-to-noise ratio, the limits of detection were 0.035 mU mL-1 for CA125, 0.087 mU mL-1 for CA19-9, and 0.016 pg mL-1 for cTnI, respectively.

20.
J Anim Sci ; 99(2)2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33515450

RESUMO

Insulin-like growth factor-I (IGF-I), growth hormone (GH), and prolactin (PRL) play important roles in milk protein synthesis, and their plasma concentrations were reported to be affected by dietary protein intake. To investigate the relationship between circulating amino acid (AA) and concentrations of these hormones, 18 Wistar rats aged 14 wk were assigned to a low (LP; 9% protein), standard (SP; 21% protein), or high-protein (HP; 35% protein) diet from parturition through day 15 of lactation. Plasma, liver, pituitary gland, skeletal muscle, and mammary gland samples were collected at the end of treatment. Circulating and hepatic IGF-I concentrations increased linearly with elevated dietary protein concentrations (P < 0.0001). Rats receiving the HP diet had higher circulating GH (P < 0.01) and pituitary PRL concentrations (P < 0.0001) but lower pituitary GH concentration (P < 0.0001) relative to those in rats receiving the LP and SP diets. Pearson correlation test performed on composed data across treatments showed that several circulating AAs were correlated with circulating and tissue concentrations of IGF-I, GH, and PRL. Multiple linear regression analyses identified Leu, Gln, Ala, Gly, and Arg as the main AAs associated with hormone responses (R2 = 0.37 ~ 0.80; P < 0.05). Rats fed the LP and HP diets had greater Igf1 and Ghr gene expression in skeletal muscle than those fed the SP diets (P < 0.01). However, LP treatment decreased Prlr mRNA abundance in mammary glands as compared with the SP and HP treatments (P < 0.05). The HP diets increased AA transporter expression (P < 0.01) but decreased mammalian target of rapamycin (P < 0.05) and 70 kDa ribosomal protein S6 kinase 1 (P < 0.01) phosphorylation in mammary glands as compared with the LP and SP diets. The results of the present study suggested that several circulating AAs mediated the effects of dietary protein supply on concentrations of IGF-I, GH, and PRL, which in turn altered the metabolism status in peripheral tissues including the lactating mammary glands.


Assuntos
Lactação , Proteínas do Leite , Aminoácidos , Animais , Dieta/veterinária , Feminino , Hormônio do Crescimento , Fator de Crescimento Insulin-Like I , Glândulas Mamárias Animais , Ratos , Ratos Wistar
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