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1.
Public Health Nutr ; : 1-11, 2020 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-32389160

RESUMO

OBJECTIVE: To assess the prevalence and to identify the associated factors of malnutrition among elderly Chinese with physical functional dependency. DESIGN: Face-to-face interviews using standardised questionnaires were conducted to collect demographic information, health-related issues and psychosocial status. Physical function was measured by the Barthel Index (BI), and nutrition status was assessed by the Mini Nutritional Assessment-Short Form. Multivariate binary logistic regression was used to assess associated factors of malnutrition. SETTING: China. PARTICIPANTS: A total of 2323 participants (aged ≥ 60 years) with physical functional dependency in five provinces in China were enrolled using a multistage cluster sampling scheme. RESULTS: The prevalence of malnutrition was 17·9 % (95 % CI 16·3, 19·4). Multivariable binary logistic regression revealed the independent risk factors of poor nutrition status were being female, older age, lower educational status, poor hearing, poor physical functional status, lack of hobbies, low religious participation, poor social support, lack of social participation and changes in social participation. The study found that the most significant independent risk factor for malnutrition was complete physical functional dependence (OR 4·46, 95 % CI 2·92, 6·82). CONCLUSIONS: The findings of the study confirm that malnutrition and the risk of malnutrition are prevalent in Chinese older adults with physical functional dependency. In addition to demographic and physical health-related factors, psychosocial factors, which are often overlooked, are independently associated with nutrition status in Chinese older adults with physical functional dependency. A holistic approach should be adopted to screen for malnutrition and develop health promotion interventions in this vulnerable population.

2.
Int J Cardiol ; 309: 101-107, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32188582

RESUMO

BACKGROUND: Growing evidence has indicated that microRNAs (miRNAs) are involved in the progression of calcific aortic valve disease (CAVD), a progressive pathological condition with no effective pharmacological therapy. This study was set out with the aim to investigate possible roles of miR-195 in CAVD. METHODS AND RESULTS: Initially, the differential expressed genes (DEGs) associated with CAVD were screened out and miRNAs potentially regulating VWF were predicted from microarray analysis. Next, we quantified VWF and miR-195 expression in isolated aortic valve interstitial cells (AVICs) and aortic valve tissues, followed by confirmation of the target relationship between miR-195 and VWF using the dual luciferase reporter assay. Furthermore, we evaluated the biological functions of miR-195 and VWF on ALP activity, cell differentiation, and the levels of miR-195, VWF, Runx2, OCN, ALP, p38 and phosphorylated p38 in AVICs. VWF was highly expressed, while miR-195 was poorly expressed in CAVD. Furthermore, miR-195 targeted VWF and negatively regulated its expression. Upregulation of miR-195 or silencing VWF could reduce ALP activity, calcified deposition, and the mRNA and protein levels of Runx2, OCN, and ALP by inhibiting the p38-MAPK signaling pathway, thereby ameliorating aortic valve calcification in vitro. CONCLUSIONS: On all accounts, miR-195 can potentially inhibit aortic valve calcification by repressing VWF and p38-MAPK signaling pathway, highlighting a theoretical basis for pharmacological treatment of CAVD.

3.
Chemosphere ; 248: 126035, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32014637

RESUMO

Epidemiologic studies show that there is a link between Bisphenol A (BPA) exposure and lung inflammation. Despite this, the molecular mechanisms are not entirely known. This study sought to determine whether exposure to BPA affected the development of ovalbumin (OVA) induced lung inflammation in adolescent female mice and whether the mechanism was related to mTOR-mediated autophagy pathway. Female 4-week-old C57BL/6 mice after one week of domestication were randomly divided into five groups (8/group): control group, OVA group, 0.1 µg mL-1 BPA + OVA group, 0.2 µg mL-1 BPA + OVA group and 0.4 µg mL-1 BPA + OVA group. BPA exacerbated airway hyperresponsiveness (AHR), induced the pathological changes in the lung, which also enhanced inflammatory cells and cytokine levels. In addition, BPA exposure affected expression of autophagy associated proteins and genes. This research results indicated that BPA aggravated OVA-induced lung inflammation and induced abnormal immune function in mice, and its mechanism was related to the activation of autophagy pathway by down-regulation expression of mTOR. These findings suggest that therapeutic strategies to target autophagy may offer a new approach for severe asthma therapy.


Assuntos
Compostos Benzidrílicos/toxicidade , Poluentes Ambientais/toxicidade , Fenóis/toxicidade , Pneumonia/induzido quimicamente , Animais , Asma/induzido quimicamente , Autofagia , Citocinas/metabolismo , Feminino , Pulmão/patologia , Camundongos , Camundongos Endogâmicos C57BL , Ovalbumina , Pneumonia/patologia , Hipersensibilidade Respiratória , Serina-Treonina Quinases TOR/metabolismo , Testes de Toxicidade
4.
Plant Mol Biol ; 102(6): 589-602, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32026326

RESUMO

Leaf angle is a key factor in plant architecture and crop yield. Brassinosteroids (BRs) regulate many developmental processes, especially the leaf angle in monocots. However, the BR signalling pathway is complex and includes many unknown members. Here, we propose that Oryza sativa BRASSINOSTEROID-RESPONSIVE LEAF ANGLE REGULATOR 1 (OsBLR1) encodes a bHLH transcription factor, and positively regulates BR signalling to increase the leaf angle and grain length in rice (Oryza sativa L.). Lines overexpressing OsBLR1 (blr1-D and BLR1-OE-1/2/3) had similar traits, with increased leaf angle and grain length. Conversely, OsBLR1-knockout mutants (blr1-1/2/3) had erect leaves and shorter grains. Lamina joint inclination, coleoptile elongation, and root elongation assay results indicated that these overexpression lines were more sensitive to BR, while the knockout mutants were less sensitive. There was no significant difference in the endogenous BR contents of blr1-1/2 and wild-type plants. These results suggest that OsBLR1 is involved in BR signal transduction. The blr1-D mutant, with increased cell growth in the lamina joint and smaller leaf midrib, showed significant changes in gene expression related to the cell wall and leaf development compared with wild-type plants; furthermore, the cellulose and protopectin contents in blr1-D were reduced, which resulted in the increased leaf angle and bent leaves. As the potential downstream target gene of OsBLR1, the REGULATOR OF LEAF INCLINATION1 (OsRLI1) gene expression was up-regulated in OsBLR1-overexpression lines and down-regulated in OsBLR1-knockout mutants. Moreover, we screened OsRACK1A as an interaction protein of OsBLR1 using a yeast two-hybrid assay and glutathione-S-transferase pull-down.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Brassinosteroides/metabolismo , Oryza/genética , Oryza/metabolismo , Folhas de Planta/anatomia & histologia , Folhas de Planta/genética , Transdução de Sinais , Regulação da Expressão Gênica de Plantas , Técnicas de Inativação de Genes , Genes de Plantas , Oryza/crescimento & desenvolvimento , Fenótipo , Reguladores de Crescimento de Planta/metabolismo , Folhas de Planta/citologia , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Transdução de Sinais/genética , Fatores de Transcrição
5.
Electrophoresis ; 41(5-6): 335-344, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31912908

RESUMO

In recent years, CE-integrated immobilized enzyme reactors (IMERs) for single-enzyme immobilization have attracted considerable attention. However, there has been little research on multienzyme immobilization in CE. Here, we introduce a method for fabricating a CE-integrated IMER, using DNA-directed immobilization to fix glucose oxidase and horseradish peroxidase in the capillary, which had been functionalized with polyamidoamine dendrimer (PAMAM). Owing to the reversibility of DNA hybridization, the reactor is capable of dynamic immobilization. Moreover, by introducing the PAMAM, the loading capacity of the IMER is greatly enhanced, and the PAMAM can spontaneously form complexes with DNA and then contribute to the efficiency and stability of the reactor. After 25 days storage, the prepared IMER ultimately retained approximately 70% of its initial activity. We also used the IMER to detect glucose, and the favorable linearity was obtained over the concentration range of 0.78-12.5 mM, with an LOD of 0.39 mM, demonstrating that the CE-integrated IMER can be applied to actual samples. We believe that this strategy can be extended to other multienzyme immobilization systems, and CE-integrated IMERs are potentially useful in a wide range of biochemical research applications.

6.
ACS Appl Mater Interfaces ; 12(6): 7510-7517, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-31971363

RESUMO

In recent years, metal-organic frameworks (MOFs) have been extensively studied as candidate enzyme immobilization platforms. However, conventional MOF-enzyme composites usually exhibit low controllability and reusability. In this study, a novel and stable strategy for enzyme immobilization was designed by use of ZIF-8 to encapsulate in situ DNA-enzyme composites on the surface of magnetic particles (MPs). The mechanism of in situ encapsulation was discussed in detail. It was found that immobilized enzymes were involved in the growth of ZIF-8, and the DNA cross-linking agents promoted the growth of ZIF-8 on the surface of MP. The thermal, chemical, and physical stabilities of horseradish peroxidase (HRP) were all significantly enhanced after in situ encapsulation. Most importantly, this strategy was proven to be a general platform that can be used to stabilize various proteins. The in situ encapsulation strategy was expanded to immobilize a cascade of enzymes, and ZIF-8@MPGOx-HRP possessed high selectivity and a wide linear range (25-500 µM) for glucose detection.

7.
Autophagy ; 16(3): 548-561, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31204557

RESUMO

Glucosamine (GlcN), a dietary supplement widely utilized to promote joint health and effective in the treatment of osteoarthritis, is an effective macroautophagy/autophagy activator in vitro and in vivo. Previous studies have shown that autophagy is required for hepatitis B virus (HBV) replication and envelopment. The objective of this study was to determine whether and how GlcN affects HBV replication, using in vitro and in vivo experiments. Our data demonstrated that HBsAg production and HBV replication were significantly increased by GlcN treatment. Confocal microscopy and western blot analysis showed that the amount of autophagosomes and the levels of autophagic markers MAP1LC3/LC3-II and SQSTM1 were clearly elevated by GlcN treatment. GlcN strongly blocked autophagic degradation of HBV virions and proteins by inhibiting lysosomal acidification through its amino group. Moreover, GlcN further promoted HBV replication by inducing autophagosome formation via feedback inhibition of mechanistic target of rapamycin kinase complex 1 (MTORC1) signaling in an RRAGA (Ras related GTP binding A) GTPase-dependent manner. In vivo, GlcN application promoted HBV replication and blocked autophagic degradation in an HBV hydrodynamic injection mouse model. In addition, GlcN promoted influenza A virus, enterovirus 71, and vesicular stomatitis virus replication in vitro. In conclusion, GlcN efficiently promotes virus replication by inducing autophagic stress through its dual effects in suppressing autophagic degradation and inhibiting MTORC1 signaling. Thus, there is a potential risk of enhanced viral replication by oral GlcN intake in chronically virally infected patients.Abbreviations: ACTB: actin beta; ATG: autophagy-related; CMIA: chemiluminescence immunoassay; ConA: concanavalin A; CQ: chloroquine; CTSD: cathepsin D; DAPI: 4',6-diamidino-2-phenylindole; EV71: enterovirus 71; GalN: galactosamine; GFP: green fluorescence protein; GlcN: glucosamine; GNPNAT1: glucosamine-phosphate N-acetyltransferase 1; HBP: hexosamine biosynthesis pathway; HBV: hepatitis B virus; HBcAg: hepatitis B core antigen; HBsAg: hepatitis B surface antigen; HBeAg: hepatitis B e antigen; HBV RI: hepatitis B replicative intermediate; IAV: influenza A virus; LAMP1: lysosomal associated membrane protein 1; LAMTOR: late endosomal/lysosomal adaptor, MAPK and MTOR activator; ManN: mannosamine; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MTORC1: mechanistic target of rapamycin kinase complex 1; PHH: primary human hepatocyte; RAB7: RAB7A, member RAS oncogene family; RPS6KB1: ribosomal protein S6 kinase B1; RRAGA: Ras related GTP binding A; RT-PCR: reverse transcriptase polymerase chain reaction; SEM: standard error of the mean; siRNA: small interfering RNA; SQSTM1/p62: sequestosome 1; UAP1: UDP-N-acetylglucosamine pyrophosphorylase 1; VSV: vesicular stomatitis virus.

8.
J Adv Nurs ; 76(2): 600-610, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31736133

RESUMO

AIMS: To investigate workplace violence and nurse outcomes by comparing gender differences. DESIGN: A secondary analysis of cross-sectional survey data. METHODS: Workplace violence was measured by four items from the International Hospital Outcome Study. Nurse outcomes were measured by tools including burnout, job satisfaction and intention to stay. We used propensity score matching to generate a sociodemographic balanced dataset of 108 male and 288 female nurses. A hypothetical relationship model was derived from the affective events theory. Comparative statistics and multi-group structural equation modelling were conducted to analyze gender differences. Data were collected in China from December 2013 - August 2014. RESULTS: Male nurses reported more workplace violence from staff and less intention to stay than females. Besides finding the mediation of burnout sharing with female nurses consistent with the affective events theory, workplace violence was directly linked to less intention to stay in male nurses. CONCLUSION: Male nurses experience more workplace violence by staff than female nurses. Besides responding emotionally to workplace violence like female nurses, male nurses also respond behaviourally. IMPACT: What problem did the study address? Gender differences in workplace violence and its relationship to nurse outcomes. What were the main findings? Male nurses experienced more workplace violence than female nurses, linked directly to less intention to stay. Workplace violence linked to less job satisfaction and intention to stay in nurses was mediated by burnout. Where and on whom will the research have impact? Gender-based prevention of and coping with workplace violence should be included in nursing training.

9.
Environ Pollut ; 257: 113633, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31761590

RESUMO

Organophosphate esters (OPEs) are widely used as flame retardants, plasticizers and defoamers and their exposure are likely associated with a number of adverse effects in humans. In this study, tris(chloroethyl) phosphate and thirteen OPE metabolites including six hydroxylated OPEs (HO-OPEs) were analyzed in 46 urine samples, collected from 8 provinces located across different regions in China. 1-Hydroxy-2-propyl bis(1-chloro-2-propyl) phosphate (BCIPHIPP) and 2-hydroxyethyl bis(2-butoxyethyl) phosphate (BBOEHEP) were major metabolites of their parent compounds with detection frequencies of 54.3%-89.1%, which were all higher than their corresponding OPE diesters (2.2%-6.5%). The urine-based estimated daily intake (EDI) of OPEs ranged from 0.06 ng/kg·bw for tris(2-butoxyethyl) phosphate (TBOEP) to 273 ng/kg·bw for 2-ethylhexyl phenyl phosphate. Analyzed with concentrations in paired dust samples, dust exposure to OPEs and their diesters may explain 0.28%-23.8% of the urine-based EDI of OPEs and the contribution of dust TBOEP was the highest. Although direct exposure to OPE diesters in dust showed a minor contribution, their intake via food and drinking water may account for a larger portion of urinary OPE metabolites. Overall, the hazard quotients of four OPEs indicated no immediate exposure risk for the investigated Chinese residents but the cumulative and long-term chronic effects involving exposure to other OPEs and OPE diesters are worth further concerns.

10.
J Colloid Interface Sci ; 560: 436-446, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31677817

RESUMO

HYPOTHESIS: The controllable transfer of colloidal particles across liquid-liquid interfaces has attracted great interests in synthesis of new materials and stabilization of emulsions. Can we find new ways of controlled transferring particles across liquid-liquid interface with reversible transfer directions and size manipulation? EXPERIMENTS: A technique of bidirectional transfer of colloidal particles in an aqueous two-phase system (ATPS) under electric pulse was developed. The influences of electric pulse, ATPS composition, surfactant concentration, ionic strength and particle size on the particle transfer were investigated systematically. FINDINGS: Under electric pulses, particles overcome the energy barrier at the liquid-liquid interface and transfer into the other phase. The action of particle transfer is determined by the voltage of electric pulse, and the transfer direction is reversible by exchanging the direction of electric pulse. The ATPS composition, surfactant concentration, ionic strength and particle size affect the particle transfer by changing the free energy of particle detachment. With this method, the targeted transfer of particles by size can be realized by controlling the strength of electric pulse. The proposed method provides a promising technique for transfer of particles across liquid-liquid interface with advantages of fast response and precise control.

11.
Med Sci Monit ; 25: 9949-9962, 2019 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-31875420

RESUMO

BACKGROUND In an atherosclerotic artery wall, monocyte-derived macrophages are the principal mediators that respond to pathogens and inflammation. The present study aimed to investigate potential genetic changes in gene expression between normal tissue-resident macrophages and atherosclerotic macrophages in the human body. MATERIAL AND METHODS The expression profile data of GSE7074 acquired from the Gene Expression Omnibus (GEO) database, which includes the transcriptome of 4 types of macrophages, was downloaded. Differentially expressed genes (DEGs) were identified using R software, then we performed functional enrichment, protein­protein interaction (PPI) network construction, key node and module analysis, and prediction of microRNAs (miRNAs)/transcription factors (TFs) targeting genes. RESULTS After data processing, 236 DEGs were identified, including 21 upregulated genes and 215 downregulated genes. The DEG set was enriched in 22 significant Gene Ontology (GO) terms and 25 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and the PPI network constructed with these DEGs comprised 6 key nodes with degrees ≥8. Key nodes in the PPI network and simultaneously involved in the prime modules, including rhodopsin (RHO), coagulation factor V (F5), and  bestrophin-1 (BEST1), are promising for the prediction of atherosclerotic plaque formation. Furthermore, in the miRNA/TF-target network, hsa-miR-3177-5p might be involved in the pathogenesis of -atherosclerosis via regulating BEST1, and the transcription factor early growth response-1 (EGR1) was found to be a potential promoter in atherogenesis. CONCLUSIONS The identified key hub genes, predicted miRNAs/TFs, and underlying molecular mechanisms may be involved in atherogenesis, thus potentially contributing to the treatment and diagnosis of patients with atherosclerotic disease.

12.
Hepatology ; 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31758709

RESUMO

Major vault protein (MVP) is up regulated during infections with hepatitis B and C virus. Here, we found that MVP deficiency inhibited hepatocellular carcinoma (HCC) development induced by di-ethyl-nitrosamine (DEN), HBx, and HCV Core. Forced MVP expression was sufficient to induce HCC in mice. Mechanistic studies demonstrate that the ubiquitin ligase Human Double Minute 2 (HDM2) forms mutual exclusive complexes either with interferon regulatory factor 2 (IRF2) or with p53. In presence of MVP, HDM2 is liberated from IRF2 leading to the ubiquitination of the tumor suppressor p53. Mouse xenograft models showed that Hepatitis B and C virus promote carcinogenesis through MVP induction, resulting in a loss of p53 mediated by HDM2. Analyses of clinical samples from chronic hepatitis B, liver cirrhosis, and HCC revealed that MVP upregulation correlates with several hallmarks of malignancy, and associates with poor overall survival. Taken together, through the sequestration of IRF2, MVP promotes an HDM2-dependent loss of p53 that promotes HCC development.

13.
ACS Appl Mater Interfaces ; 11(47): 44413-44420, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31691552

RESUMO

Two-dimensional materials have been extensively investigated in the fields of electrochemical sensors, field-effect transistors, and other electronic devices due to their large surface areas, high compatibility with device integration, and so on. Conventional electrodes, such as precious metal layers that are deposited on polymer or silicon wafers, have gradually revealed increasing difficulties in adapting to various device structures, especially for two-dimensional materials, which prefer high exposure of surface atoms. Here, we demonstrate a tailorable metal-ceramic (Cu-TiC0.5) layered structure as novel electrodes with high mechanical property and conductivity and fabricate a highly sensitive gas sensor with graphene lying on this proposed electrodes. The Cu-TiC0.5 layered structure exhibits remarkably high tensile yield strength and compressive yield strength, which increase 7 and 8 times than those of the pure copper, respectively. Meanwhile, excellent flexibility and conductivity could also be obtained with the further thinning of the Cu-TiC0.5 layered composite, which shows its potential applications in flexible electronics. Finally, we demonstrated that a graphene-based gas sensor fabricated on tailored metal-ceramic electrodes was ultrasensitive and robust, which benefits from the good thermal conductivity and peculiar gas channels etched on the surface of copper alloy electrodes.

14.
J Am Chem Soc ; 141(46): 18467-18474, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31656065

RESUMO

The fast light-responsive dithienylethenes (DTEs) are one of the most attractive photochromic families because of their excellent thermal irreversibility and fatigue resistance. However, the all-visible-light-activated DTE system still remains challenging because most of them require the harmful high-energy ultraviolet light to trigger their photocyclization reaction. Here, we have for the first time borrowed a specific intramolecular proton transfer (IPT) process and rationally designed a series of all-visible-light-driven DTEs. Incorporating the IPT-functional group to DTE unit gives rise to an extra absorption band with a distinct red shift, which enables the photocyclization of DTEs under stimuli of visible light at 450 nm, as well as ensuring the desirable photoswitching efficiency. The isomerization from OH form to NH form induced by IPT can decrease the energy gap for excitation and photocyclization, thereby affording the all-visible-light-triggered photochromic performance, which can not only work well in a polar solvent system but also show its effectiveness in polymeric gel systems. In this regard, we can provide a general and reliable platform to construct all-visible-light-driven DTEs with excellent reversible photoswitching and broad applicability, especially with avoiding the use of harmful ultraviolet light to induce their photocyclization.

15.
Environ Int ; 133(Pt A): 105143, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31630067

RESUMO

Phthalic acid esters (PAEs) are one important category of additives in plastics, which are ubiquitous products of e-waste recycling areas, where PAEs are released to the environment intensively and higher exposure level is expected for the employees. This study investigated human exposure levels of PAEs in an e-waste recycling area (Ziya Circular Economy Park (ZCEP) in Tianjin, China) with intending to explore the impacts of residence spatial variation and dismantling manipulation mode. We collected 157 urine samples from three sites around ZCEP with different distances from the core dismantling site and urinary phthalate metabolites (mPAEs) concentrations were measured and were compared among these three sites. The exposure levels of PAEs exhibited spatial variation according to the distance from the core dismantling site, and urinary median ∑mPAEs concentrations (389 ng/mL) of the employees in ZCEP were significantly higher than those of residents in Ziya town (285 ng/mL) and the downtown of Jinghai district (207 ng/mL) (p < 0.05). Moreover, PAEs exposure levels were significantly affected by the manipulation modes in the e-waste recycling area and the urinary median ∑mPAEs concentrations in the employees of family workshops (401 ng/mL) were significantly higher than those in plants with centralized management (298 ng/mL). There were obvious differences on the urinary median mPAEs concentrations between subgroups based on age, BMI, and sex; however, no significant statistical associations were found between PAEs exposure levels and these socio-demographic indices (p > 0.05). Besides, there was no correlation between exposure levels of different PAEs and their physicochemical parameters like the logKow (p > 0.05).

16.
Artigo em Inglês | MEDLINE | ID: mdl-31532862

RESUMO

Unprecedented dual aggregation-induced emission (AIE) behavior based on a steric-hindrance photochromic system is presented, with incorporation one or two bulky aryl groups, resulting in different flexibleness. The dual AIE behavior of open and closed isomers can be explained by restriction of intramolecular rotation (RIR), restriction of intramolecular vibration (RIV), and intermolecular stacking. The large bulky benzothiophene causes restricted rotation, enhancing the emission of open form in solution and weak π-π molecular packing, thereby efficiently enhancing the luminescence performance in the solid state. With incorporation of two large bulky benzothiophene groups, BBTE possesses the most outstanding AIE activity, undergoing highly efficient and reversible off-to-on fluorescence in film upon alternating UV and visible light irradiation along with excellent fatigue resistance. The off-to-on fluorescent photoswitch is successfully established in super resolution imaging.

17.
Medicine (Baltimore) ; 98(36): e16937, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31490377

RESUMO

Metadherin (MTDH), also known as astrocyte elevated gene-1 (AEG-1), is an oncoprotein closely related to the development of breast cancer. However, few studies have been done on the expression and clinical significance of MTDH in human epidermal growth factor receptor-2 (HER-2) positive breast cancer patients.This study aimed to investigate the expression of MTDH in locally advanced HER-2 positive breast cancer, and evaluate the clinical significance of MTDH in predicting the prognosis of patients with HER-2 positive advanced breast cancer who received the neoadjuvant chemotherapy plus trastuzumab.In 144 HER-2 positive breast cancer tissues, 79 cases showed high expression of MTDH and 65 cases showed low expression. The expression of MTDH in locally advanced HER-2 positive breast cancer tissues was correlated with TNM stage, lymph node metastasis, Miller-Payne (MP) grade, and pathologic complete response (pCR) status (P < .05), but was not correlated with patient age, estrogen receptor (ER) expression level, progesterone receptor (PR) expression level, and Ki-67 expression level (P > .05). Kaplan-Meier univariate analysis revealed a negative correlation between MTDH expression and the disease-free survival (DFS) and overall survival (OS) in the post-operative patients with locally advanced HER-2 positive breast cancer (log rank test: P < .001). By using the COX proportional hazard regression model, it was found that MTDH expression, TNM stage, lymph node metastasis, and Ki-67 expression were closely related to DFS in patients. The hazard ratio (HR) of high MTDH expression was 1.816 (95% CI: 1.165-2.829). In addition, MTDH expression, TNM stage, and lymph node metastasis were also closely related to the OS of patient. The HR of the high expression of MTDH was 2.512 (95% CI: 1.472-4.286). The expression of MTDH in tumor tissues of patients with HER2-positive locally advanced breast cancer was significantly elevated, which was related to the poor pathological features.High MTDH expression was closely correlated with poor prognosis of patients and was an important factor affecting tumor progression.


Assuntos
Antineoplásicos Imunológicos/uso terapêutico , Neoplasias da Mama/mortalidade , Neoplasias da Mama/patologia , Moléculas de Adesão Celular/biossíntese , Receptor ErbB-2/biossíntese , Trastuzumab/uso terapêutico , Adulto , Fatores Etários , Idoso , Neoplasias da Mama/tratamento farmacológico , Quimioterapia Adjuvante , Intervalo Livre de Doença , Feminino , Humanos , Estimativa de Kaplan-Meier , Metástase Linfática , Pessoa de Meia-Idade , Gradação de Tumores , Estadiamento de Neoplasias , Prognóstico , Receptores Estrogênicos/biossíntese , Receptores de Progesterona/biossíntese
18.
J Agric Food Chem ; 67(38): 10563-10576, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31487171

RESUMO

Sulfur (S) metabolism plays a vital role in Cd detoxification, but the collaboration between melatonin biosynthesis and S metabolism under Cd stress remains unaddressed. Using exogenous melatonin, melatonin-deficient tomato plants with a silenced caffeic acid O-methyltransferase (COMT) gene, and COMT-overexpressing plants with cosuppression of sulfate transporter (SUT)1 and SUT2 genes, we found that melatonin deficiency decreased S accumulation and aggravated Cd phytotoxicity, whereas exogenous melatonin or overexpression of COMT increased S uptake and assimilation, resulting in an improved plant growth and Cd tolerance. Melatonin deficiency promoted Cd translocation from root to shoot, but COMT overexpression caused the opposite effect. COMT overexpression failed to compensate the functional hierarchy of S when its uptake was inhibited by cosilencing of transporter SUT1 and SUT2. Our study provides genetic evidence that melatonin-mediated tolerance to Cd is closely associated with the efficient regulation of S metabolism, redox homeostasis, and Cd translocation in tomato plants.


Assuntos
Cádmio/metabolismo , Lycopersicon esculentum/metabolismo , Melatonina/metabolismo , Enxofre/metabolismo , Transporte Biológico , Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum/genética , Lycopersicon esculentum/crescimento & desenvolvimento , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/metabolismo , Oxirredução , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteína O-Metiltransferase/genética , Proteína O-Metiltransferase/metabolismo
19.
World J Clin Cases ; 7(16): 2346-2351, 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31531330

RESUMO

BACKGROUND: Surgical treatment for large carotid body tumor (CBT), particularly the Shamblin III type, is challenging and rarely reported. CASE SUMMARY: In July 2014, a 63-year-old woman presented to our hospital with a large CBT (130 mm × 60 mm × 70 mm). The lesion was hypervascular, spanned from the first to the seventh cervical vertebra, and adhered to the right common carotid artery (CCA), internal carotid artery (ICA) and external carotid artery (ECA). The resection was carried out in a hybrid operating theatre. First, we used Onyx gel to embolize the feeding artery. An ICA balloon was used to prevent gel entry into the ICA. After shrinkage and hardening of the CBT, we quickly resected the CBT as well as a part of the ECA that adhered to the CBT. A vascular shunt was inserted between CCA and ICA, and the part where the ICA was cut off from the CCA was directly sutured. A follow-up at four years later showed no neurological damage. CONCLUSION: For large hypervascular CBT, embolization of the feeding artery prior to resection is helpful. The hybrid operating theatre is the ideal platform to carry out such operations.

20.
Med Sci Monit ; 25: 6675-6690, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31488807

RESUMO

BACKGROUND Research on microparticles is rapidly evolving and has extended to the field of many diseases. It is unclear whether microparticles can be stored frozen. In this study, our goal was to verify whether cryopreservation had an effect on the properties of the microparticles. MATERIAL AND METHODS We obtained C57BL/6J mouse-derived microparticles by grinding and gradient centrifugation. The specimens were divided into 2 groups: without dimethyl sulfoxide and with dimethyl sulfoxide. The microparticles were then stored at 25°C, 4°C, -20°C, -80°C, and -196°C for 0.5 days, 1 day, 3 days, 5 days, and 7 days. We tested whether the concentration, coagulation function, diameter distribution, and morphology of the microparticles in the 2 groups changed compared to those of a fresh sample. RESULTS We discovered that the concentrations of total microparticles, annexin V-positive microparticles, and brain-derived microparticles changed with freezing. The coagulation function, morphology, and size distribution of the microparticles were also affected by cryopreservation. Finally, there was no difference in the effects of cryopreservation on microparticles between the dimethyl sulfoxide group and the dimethyl sulfoxide-free group. CONCLUSIONS This study suggests that cryopreservation has diverse effects on microparticles within 1 week and that dimethyl sulfoxide has no protective effect on cryopreserved microparticles. Therefore, microparticles should be used fresh for future studies, and they should not be cryopreserved with or without dimethyl sulfoxide.


Assuntos
Micropartículas Derivadas de Células/química , Micropartículas Derivadas de Células/ultraestrutura , Coagulantes/farmacologia , Criopreservação , Tamanho da Partícula , Animais , Anexina A5/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Nanopartículas/química , Nanopartículas/ultraestrutura , Temperatura
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