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1.
Artigo em Inglês | MEDLINE | ID: mdl-33606014

RESUMO

CONTEXT: Aggrecan, encoded by ACAN gene, is the main proteoglycan component in the extracellular cartilage matrix. Heterozygous mutations in ACAN have been reported to cause idiopathic short stature. However, the prevalence of ACAN pathogenic variants in Chinese short stature patients and clinical phenotypes remain to be evaluated. OBJECTIVE: We sought to determine the prevalence of ACAN pathogenic variants among Chinese short stature children and characterize the phenotypic spectrum and their responses to growth hormone (GH) therapies. PATIENTS AND METHODS: Over 1000 unrelated short stature patients ascertained across China were genetically evaluated by Next-generation sequencing (NGS)-based test. RESULT: We identified 10 novel likely pathogenic variants and 2 recurrent pathogenic variants in this cohort. None of ACAN mutation carriers exhibited significant dysmorphic features or skeletal abnormities. The prevalence of ACAN defect is estimated to be 1.2% in the whole cohort, it increased to 14.3% among those with advanced bone age and to 35.7% among those with both advanced bone age and family history of short stature. Nonetheless, five out of eleven ACAN mutation carries had no advanced bone age. Two individuals received growth hormone therapy with variable levels of height SDS improvement. CONCLUSION: Our data suggested that ACAN mutation is one of the common causes of Chinese pediatric short stature. Although it has a higher detection rate among short stature patients with advanced bone age and family history, part of affected probands presented with delayed bone age in Chinese short stature population. The growth hormone treatment was moderately effective for both individuals.

2.
PLoS One ; 16(1): e0245136, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33417597

RESUMO

In the present study, we examined self-rated health as a mediator between physical health conditions (chronic diseases and functional disability) and depressive symptoms in older Chinese and Korean Americans. Using harmonized data (N = 5,063) from the Population Study of Chinese Elderly (PINE) and the Study of Older Korean Americans (SOKA), we tested direct and indirect effect models. In both groups, chronic diseases and functional disability were closely associated with negative ratings of health and symptoms of depression. Analyses with the PROCESS macro showed that the effect of chronic diseases and functional disability on depressive symptoms was mediated by self-rated health in both groups; the indirect effect was greater in the Korean American sample than in the Chinese American sample. These findings contribute to the understanding of the psychological mechanisms that underlie the mind-body connection and highlight the potential importance of subjective health assessment as a useful tool for health promotion.

3.
Spectrochim Acta A Mol Biomol Spectrosc ; 249: 119300, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33348094

RESUMO

Field monitoring technology plays a vital role for returning the animal manure back to the cropland with high-efficiency and accuracy, particular in the complex rotation system of manure management in Chinese intensive farms. The comprehensive quantitative analysis models were proposed and built for determining the content of the nitrogen (N) and the phosphorus (P) through the whole chain of manure management in different dairy farms under multiple conditions. 249 manure samples were collected from 31 intensive dairy farms in Tianjin both in summer and autumn. The effect of seasons on the distribution characteristics of the N and P in the manure was analyzed. Near infrared spectra were collected and principal component analysis (PCA) was performed. Partial least squares (PLS) was used to establish the intra-season and inter-season models. It was found that the contents of the N and P in the manure varied with seasons. The prediction performance of intra-season models was better than that of inter-season models. Fusion model of two seasons were also established. The coefficient of determination of external validation (R2pred) for the N and P were 0.972 and 0.901, respectively. The residual predictive deviations (RPD) were 5.98 and 3.18, respectively. The results showed that the fusion model could enhance the universality and stability for predicting the N and P contents through the whole chain of manure management under the influence of various factors. The study not only supports for the development of on-spot detecting instrument but also guides for the rational recycling of manure in practice as well.

4.
Res Aging ; 43(1): 37-46, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32672093

RESUMO

A broad literature has explored racial disparities in cognitive aging. Research incorporating sociocultural factors would provide a more comprehensive understanding of minority aging. This study aims to investigate the role of family typology in cognition among U.S. Chinese immigrants. Data were derived from the Population Study of Chinese Elderly in Chicago (PINE). Family typology included tight-knit, unobligated ambivalent, commanding conflicted, and detached typologies. Cognition was evaluated by global cognition, episodic memory, working memory, executive function, and mini-mental state examination (MMSE). Linear and quantile regressions were used. Older adults with detached and commanding conflicted typologies reported lower global cognition than those with unobligated ambivalent typology. Detached, commanding conflicted, and tight-knit typologies were associated with poorer performance in episodic memory, working memory and MMSE than unobligated ambivalent typology, respectively. Social service providers could be aware of multifaceted family relationships when developing interventions for cognitive function and understand family typology as a whole.

5.
BMC Med Genomics ; 13(1): 181, 2020 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-33276791

RESUMO

BACKGROUND: Wolf-Hirschhorn syndrome is a well-characterized genomic disorder caused by 4p16.3 deletions. Wolf-Hirschhorn syndrome patients exhibit characteristic facial dysmorphism, growth retardation, developmental delay, intellectual disability and seizure disorders. Recently, NSD2 gene located within the 165 kb Wolf-Hirschhorn syndrome critical region was identified as the key causal gene responsible for most if not all phenotypes of Wolf-Hirschhorn syndrome. So far, eight NSD2 loss of function variants have been reported in patients from different parts of the world, all were de novo variants. METHODS: In our study, we performed whole exome sequencing for two patients from one family. We also reviewed more NSD2 mutation cases in pervious literature. RESULTS: A novel loss of function NSD2 variant, c.1577dupG (p.Asn527Lysfs*14), was identified in a Chinese family in the proband and her father both affected with intellectual disability. After reviewing more NSD2 mutation cases in pervious literature, we found none of them had facial features that can be recognized as Wolf-Hirschhorn syndrome. In addition, we have given our proband growth hormone and followed up with this family for 7.5 years. CONCLUSIONS: Here we reported the first familial NSD2 variant and the long-term effect of growth hormone therapy for patients. Our results suggested NSD2 mutation might cause a distinct intellectual disability and short stature syndrome.

6.
Sci China Life Sci ; 63(12): 1776-1785, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33351176

RESUMO

Resolution is undoubtedly the most important parameter in optical microscopy by providing an estimation on the maximum resolving power of a certain optical microscope. For centuries, the resolution of an optical microscope is generally considered to be limited only by the numerical aperture of the optical system and the wavelength of light. However, since the invention and popularity of various advanced fluorescence microscopy techniques, especially super-resolution fluorescence microscopy, many new methods have been proposed for estimating the resolution, leading to confusions for researchers who need to quantify the resolution of their fluorescence microscopes. In this paper, we firstly summarize the early concepts and criteria for predicting the resolution limit of an ideal optical system. Then, we discuss some important influence factors that deteriorate the resolution of a certain fluorescence microscope. Finally, we provide methods and examples on how to measure the resolution of a fluorescence microscope from captured fluorescence images. This paper aims to answer as best as possible the theoretical and practical issues regarding the resolution estimation in fluorescence microscopy.

7.
Ren Fail ; 42(1): 1135-1141, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33179540

RESUMO

OBJECTIVE: The present study investigated whether hemodialysis (HD) patients exhibit future memory impairment (PM; the capability of remembering to perform expected future actions) and exploring relevant factors of PM task performance. METHODS: Sixty HD patients and 60 healthy controls matched by age are enrolled in the Mini-Mental State Examination (MMSE), Finger Span Test (DST), Oral Fluency Test (VFT), Ray Auditory Oral Learning Test (RAVLT), Received Stroop Color Word Interference Test (SCWT), and event-based PM (EBPM) and time-based PM (TBPM). RESULTS: There were no significant difference between the patients and controls in the DST-Forward digit span (9.00 ± 1.25 versus 8.97 ± 1.33, p = 0.96), the DST-Backward digit span (5.23 ± 1.98 versus. 4.60 ± 1.65, p = 0.11), the RAVLT of delayed recall (7.28 ± 2.36 versus 6.87 ± 3.33, p = 0.09) and the VFT for animals (16.70 ± 3.50 versus 17.68 ± 5.45, p = 0.56). By comparison, patients had a much worse performance than controls on the MMSE (29.10 ± 0.84 versus 28.33 ± 0.77, p < 0.001), the RAVLT of total recall (44.47 ± 5.82 versus 40.03 ± 10.46, p < 0.001) and delayed recognition (6.93 ± 1.49 versus 5.4 ± 1.33, p < 0.001), the SCWT reaction time in reading (6.47 ± 1.05 versus 7.47 ± 1.86, p < 0.001), color naming (9.07 ± 1.29 versus 11.43 ± 2.34, p < 0.001), interference (8.78 ± 1.92 versus 10.22 ± 2.91, p < 0.001) and inhibition/switching (14.53 ± 2.90 versus 19.85 ± 4.69, p < 0.001), the VFT for fruit (17.47 ± 3.18 versus 15.92 ± 4.56, p < 0.001), the EBPM task (7.85 ± 0.40 versus 7.08 ± 1.43, p = 0.01), and the TBPM task (3.30 ± 1.31 versus 2.26 ± 1.82, p < 0.001). CONCLUSIONS: Our results suggest that EBPM and TBPM are impaired in HD patients and that PM may be applied to help evaluate cognitive dysfunction in HD patients.

8.
J Aging Health ; : 898264320976772, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33249977

RESUMO

Objectives: Elder mistreatment (EM) is associated with worse physical health and psychological well-being, but little is known regarding its cognitive consequences. Methods: Data were derived from the T1-T2 PINE (n = 2713). EM was measured by psychological, physical, and sexual mistreatment; financial exploitation; and caregiver neglect. Cognitive function was evaluated by global cognition, episodic memory, working memory, processing speed, and Mini-Mental State Examination. Linear regression was used. Results: At baseline, physical mistreatment and caregiver neglect were associated with lower global cognition and cognitive domains, while psychological mistreatment was associated with higher cognitive function. Older adults with incident psychological mistreatment, physical mistreatment, and caregiver neglect had a faster decrease in global cognition and cognitive domains over 2 years. Discussion: This study is among the first to examine the association between different forms of EM and cognitive change. These findings provide a basis for engaging ethnic minorities with EM to maintain cognitive health.

9.
J Infect Dev Ctries ; 14(9): 963-967, 2020 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-33031081

RESUMO

INTRODUCTION: COVID-19 has become a global health security issue, it has caused more than half a million deaths worldwide so far, the treatment strategies are the most concerned issues for clinicians. In this study, the treatments and outcomes in 40 pediatric patients diagnosed with COVID-19 and treated with different drugs were evaluated. METHODOLOGY: All cases were diagnosed with COVID-19 nucleic acid positive by using RT-PCR or clinical manifestations, imaging specific characteristics and epidemiological clinical diagnosis. The biological information and first symptom of all cases were collect. A variety of treatments were employed and the outcomes were evaluated by Cox regression analysis. Multivariable analysis was performed to evaluate cure rate at 14 days with different drug treatment. RESULTS: The average length of hospital stay was 10.4 days. The cure rate was increased with the treatment time extended and 90% of pediatric patients were cured and discharged after 14 days' treatment. And multivariable analysis results proved that none of the covariates were related to the cure rate at 14 days with different drug treatment since p values were over 0.05. CONCLUSIONS: Multivariable analysis suggested that the present drug treatments cannot significantly shorten the clinical cure time and improve the cure rate of children with COVID-19.


Assuntos
Antivirais/uso terapêutico , Betacoronavirus , Infecções por Coronavirus/tratamento farmacológico , Pneumonia Viral/tratamento farmacológico , Adolescente , Betacoronavirus/isolamento & purificação , Criança , Pré-Escolar , China , Técnicas de Laboratório Clínico , Infecções por Coronavirus/diagnóstico , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Análise Multivariada , Pandemias , Pneumonia Viral/diagnóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento
10.
Trials ; 21(1): 844, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33046128

RESUMO

BACKGROUND: Traditional Chinese medicine (TCM) has a long history of use in breast cancer, but lacking systematic evidence to support its clinical benefits. In this study, we evaluated the prophylactic and therapeutic effects of moxibustion combined with decoctions for treating chemotherapy-induced myelosuppression (CIM) in early-stage breast cancer patients. METHODS: This is a randomized controlled clinical trial single-blinded for TCM decoction but not moxibustion. Patients are equally divided into the control group without decoction and moxibustion treatment (control), the decoction+moxibustion group (MD), and the placebo+moxibustion group (MP), according to the following stratification factors: age (below 40s, 40s, 50s, and 60s or above), chemotherapy regimen (anthracyclines, taxanes, anthracyclines+taxane, and others), and chemotherapy strategy (adjuvant and neoadjuvant). The TCM decoction is Wenshen Shengbai Decoction. The anticipated sample size is 462 cases (154 cases in each group). All participants are expected to treat with chemotherapy and recombinant human granulocyte colony-stimulating factor (rhG-CSF). The primary outcomes include the proportion of patients with relief of leukopenia and/or neutropenia, the myelosuppression-associated serious adverse event including grade 3-4 leukopenia and/or neutropenia, and febrile neutropenia, and the dose of rhG-CSF. The secondary outcomes include chemotherapy adherence, stratified analysis, adverse reactions, quality of life by EORTC Breast-Cancer-Specific Quality of Life Questionnaire including EORTC QLQ-C30 (V3.0) and QLQ-BR23, TCM Constitution, and 3-year disease-free survival and overall survival. Baseline information including age, surgical approach, chemotherapy regimen and strategy, pathological stage, and molecular subtype will be recorded. DISCUSSION: This will be the first randomized controlled trial to evaluate the efficacy of moxibustion combined with TCM decoction in treating CIM in early-stage breast cancer patients, aiming to standardize the TCM decoction and moxibustion method, thus providing evidence for its clinical benefit. TRIAL REGISTRATION: chictr.org.cn ChiCTR-INR-16009557 . Registered on 23 October 2016.

11.
Talanta ; 219: 121329, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32887063

RESUMO

Simplicity, sensitivity and high throughput are the qualities researcher always difficultly pursue in the screening surveillance assay for food contaminants. Herein, coupled with a common gel imager as a signal reader, a novel magnet-actuated droplet microfluidic immunosensor without any complicated manipulation system was successfully fabricated for the convenient and sensitive detection of cyanobacteria toxin microcystin-LR (MC-LR) in aquatic products. The proposed sensor demonstrated three superiorities compared to the extensively used microfluidic systems: 1) simple but effective operation only by magnetic force rather than inconvenient actuating devices; 2) high throughput parallel detection on a 15-channels chip; 3) convenient signal readout on an ordinary laboratorial gel imager. This gel imager-assisted magnet-actuated droplet microfluidic immunosensor exhibited a limit of detection as low as 6.0 × 10-4 µg/L with high specificity. The average recoveries were ranged from 92.0% to 96.6% in spiked fish and prawn samples, and showed a good agreement with confirmation method LC-MS/MS, suggesting the proposed method to be an ideal surveillance assay tool for the MC-LR monitoring in aquatic products.

12.
Life Sci ; 261: 118368, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32888940

RESUMO

AIMS: Colorectal cancer (CRC) is one of the most common cancers with poor prognosis worldwide. The advent of immunotherapy has greatly improved survival in refractory patients of CRC. In this study, we aimed to identify reliable immune classification and biomarkers that predict immunotherapeutic responses in CRC patients. MATERIALS AND METHODS: Based on transcriptome profiles of two publicly available CRC datasets, we performed single-sample gene set enrichment analysis (ssGSEA) to calculate the relative abundance of 29 immune-related items of each sample. Unsupervised clustering was used to classify CRC patients. Furthermore, an immune prognostic signature was constructed using the least absolute shrinkage and selection operator (LASSO) Cox regression analysis. KEY FINDINGS: The CRC patients were clustered into high, medium, low immune infiltration subtypes based on the immune landscape. There was significant heterogeneity among the three subtypes. The high immune infiltration group showed higher expression of programmed cell death-ligand 1 and better prognosis than the median and low immune infiltration groups. Furthermore, we constructed a 7-immune-related prognostic gene signature and found that the signature had high predictive value and was superior to other clinicopathological parameters. Finally, the correlation analysis of the signature with immune cell infiltration and immune checkpoint molecules suggested that the signature had the potential to assess the immunotherapeutic responses of CRC patients. SIGNIFICANCE: The immune landscape and prognostic signature of CRC contribute to a deeper understanding of the tumor microenvironment and guide accurate immunotherapy.


Assuntos
Neoplasias Colorretais/imunologia , Neoplasias Colorretais/terapia , Imunoterapia , Neoplasias Colorretais/patologia , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Fenótipo , Valor Preditivo dos Testes , Prognóstico
13.
MethodsX ; 7: 101057, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32983922

RESUMO

In natural environment, the microorganisms are exposed to complex mixtures of contaminants, including manufactured nanoparticles and their aggregates. Evaluation of the toxicant accumulation in biota exposed to such cocktails is a challenging task because the microorganisms need to be separated from nanomaterial aggregates often of a comparable size. We propose a method for separation of TiO2 aggregates from green microalga Chlamydomonas reinhardtii and subsequent determination of cellular Hg concentration in algae exposed to mixture of Hg with nanoTiO2, known also to adsorb Hg. The method is based on differences in specific weight of algae and TiO2 aggregates, using medium speed centrifugation on a step gradient of sucrose. The efficiency of the separation method was tested with nanoTiO2 of three different primary sizes at four concentrations: 2, 20, 100 and 200 mg L-1. The method gives a possibility to separate nanoTiO2 and their aggregates from the algae with a mean recovery of 83.3% of algal cells, thus allowing a reliable determination of Hg accumulation by microalgae when co-exposed to Hg and nanoTiO2. • A rapid and reliable method to separate algal cells and nanoparticle aggregates of comparable size. • A method to measure the cellular amount of Hg in green alga co-exposed to Hg and nanoTiO2.

14.
J Elder Abuse Negl ; : 1-19, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32886054

RESUMO

OBJECTIVE: This study aimed to examine the associations between different types of elder mistreatment, anxiety symptoms, and depressive symptoms among U.S. Chinese older adults. METHODS: Data were derived from the Population Study of Chinese Elderly (PINE), a study of Chinese older adults aged 60 years and above in the greater Chicago area from 2011-2013. Measurements include elder mistreatment screening tools, Hospital Anxiety and Depression Scale, and Patient Health Questionnaire-9. Negative binomial regressions and logistic regressions were performed. RESULTS: Participants with any mistreatment, psychological mistreatment, physical mistreatment, financial exploitation, and caregiver neglect were more likely to have anxiety symptoms and depressive symptoms. Sexual mistreatment was not associated with symptoms of anxiety and depression. CONCLUSION AND IMPLICATIONS: The rate of psychological distress differs based on the types of mistreatment among U.S. Chinese older adults. The findings underline the need for public and community awareness and improved education for health care professionals.

15.
Carbohydr Polym ; 248: 116732, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32919549

RESUMO

Ion-imprinted thermosensitive chitosan derivative (ITC) was successfully synthesized through a novel gradient heating process towards applications in heavy metal remediation, using Cu2+ template paired with N-isopropylacrylamide (NIPAM) monomer. SEM, DSC, FTIR and Raman spectroscopy were used to characterize the structure and properties of ITC. The phase change temperature (PCT) of ITC was 35.31 °C in the heating cycle, whereas in the cooling cycle the PCT for ITC was 26.27 °C. The adsorption capacity of ITC for Cu2+ at 45 °C (> PCT) was 88.94 mg/g. Desorption rate of 98.90 % was achieved after washing with ultra-pure water at 20 °C (< PCT). An adsorption capacity of 85 mg/g and a desorption rate of 97 % sustained after five cycles reuse. The adsorption capacity for Cu2+ was measured at 80.23 mg/g under other seven interfering metal ions. The adsorption kinetics and adsorption isotherm fitted pseudo-second-order and Langmuir equation, respectively.

16.
Blood Coagul Fibrinolysis ; 31(8): 506-510, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32852327

RESUMO

: Hemophilia A is an X-linked hemorrhagic disorder caused by deficiency or dysfunction of the coagulation factor VIII (FVIII), and a great variety of mutations in the factor VIII gene (F8) are identified. We aimed to identify the genetic defects of the F8 gene in a Chinese patient with moderate hemophilia A. We have identified a novel intronic variant in the hemophilia A patient by DNA sequence analysis, cDNA sequencing, and TA clone sequencing. An intronic variant, c.5816-1G>A, was identified and the cDNA sequencing confirmed the pathogenicity of the transition. TA clone sequencing showed that the splicing mutation produced two aberrant premRNA skipping exons (18 and exon 18 + 19, respectively). These aberrant mRNA forms maintain the reading frame and are predicted to code for deleted FVIII isoforms and the shorter abnormal transcript accounted for one-eighth of the total mRNA. There was a new unreported transcript with E22 spliced out in healthy individuals and our patient, whose specific functions need to be determined in further studies. Our study widens the mutation spectrum of the F8 gene. In addition, the study findings could provide the opportunity to reveal alternative splicing patterns.

17.
Int Immunopharmacol ; 88: 106868, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32771948

RESUMO

BACKGROUND: Colorectal cancer (CRC) is one of the most common malignant neoplasms worldwide. Although the significant efficacy of immunotherapy has been shown, only limited CRC patients benefit from it. Therefore, we aimed to establish a prognostic signature based on immune-related genes (IRGs) to predict overall survival (OS) and the potential response to immunotherapy in CRC patients. METHODS: Gene expression profiles and clinical information of CRC patients were collected from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. The prognostic signature composed of IRGs was established using univariate Cox regression and the least absolute shrinkage and selection operator (LASSO) regression analysis. CIBERSORT was used to estimate the immune cell infiltration. RESULTS: A total of 24 survival-related IRGs were identified from 247 differentially expressed IRGs. Then, 16 IRGs were selected to establish the prognostic signature that stratified the patients into the high-risk and low-risk groups with statistically different survival outcomes. The AUCs of the time-dependent ROC curves indicated that the signature had a strong predictive accuracy in internal and external validation sets. Multivariate cox regression analysis suggested that the signature could also act as an independent prognostic factor for OS. The low-risk group had a higher proportion of immune cell infiltration than the high-risk group, such as CD4 memory resting T cells, activated dendritic cells, and resting dendritic cells. In addition, patients in the high-risk group exhibited higher tumor mutation burden and BRAF mutation. CONCLUSION: We developed an immune-related prognostic signature to predict the OS and immune status in CRC patients. We believed that our signature is conducive to better stratification and more precise immunotherapy for CRC patients.

18.
J Drug Target ; : 1-13, 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32795132

RESUMO

It is important to enhance penetration depth of nanomedicine and realise rapid drug release simultaneously at targeted tumour for improving anti-tumour efficiency of chemotherapeutic drugs. This project employed sodium alginate (Alg) as matrix material, to establish tumour-responsive nanogels with particle size conversion and drug controlled release functions. Specifically, tumour-targeting peptide CRGDK was conjugated with Alg first (CRGDK-Alg). Then, doxorubicin (DOX) was efficiently encapsulated in CRGDK-FeAlg nanogel during the cross-linking process (CRGDK-FeAlg/DOX). This system was closed during circulation. Once reaching tumour, the particle size of nanogels was reduced to ∼25 nm, which facilitated deep penetration of DOX in tumour tissues. After entering tumour cells, the size of nanogels was further reduced to ∼10 nm and DOX was released simultaneously. Meanwhile, FeAlg efficiently catalysed H2O2 to produce •OH by Fenton reaction, achieving local chemodynamic therapy without O2 mediation. Results showed CRGDK-FeAlg/DOX significantly inhibited tumour proliferation in vivo with V/V0 of 1.13 after treatment, significantly lower than that of control group with V/V0 of 4.79.

19.
Nano Lett ; 20(9): 6510-6519, 2020 09 09.
Artigo em Inglês | MEDLINE | ID: mdl-32786929

RESUMO

Hepatic ischemia-reperfusion injury (IRI), which mainly results from excessive reactive oxygen species (ROS) generated by a reperfusion burst of oxygen, has long been a major cause of liver dysfunction and failure after surgical procedures. Here, a monodispersed hydrophilic carbohydrate-derived nanoparticle (C-NP) was synthesized as a nanoantioxidant that could effectively prevent hepatic IRI. The spherical C-NPs had a size of ∼78 ± 11.3 nm covered with polar surface groups. They were well dispersible in water with good colloidal stability, nontoxicity, and good ROS scavenging capability. The C-NPs also exhibited good circulation lifetime, effective delivery to liver, and gradual degradability with an ability to assist the IRI group maintaining a normal and healthy liver status. The pathology mechanism of C-NPs in hepatic IRI was confirmed to be scavenging of excessive ROS by C-NPs. The effective therapeutic treatment of C-NPs in living animals revealed a great potential in clinical prevention for hepatic IRI.

20.
Front Genet ; 11: 592, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32625235

RESUMO

CHARGE syndrome is a life-threatening disease caused by mutations of chromodomain helicase DNA-binding protein 7 gene (CHD7). The disease is characterized by a pattern of congenital anomalies that involve multiple organs. In this study, five patients were diagnosed as CHARGE syndrome with CHD7 mutations by whole exome sequencing. Although the clinical phenotypes of probands are highly variable and typical symptoms such as coloboma and choanal atresia are not commonly manifested in this cohort, they all presented congenital heart defects. Of note, dyspnea is the most prominent symptom in all five neonatal patients, suggesting that dyspnea might be a phenotypic clue of CHARGE syndrome.

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