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1.
Food Chem ; 411: 135483, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36708641

RESUMO

Formaldehyde (FA) is widely used as an adhesion promoter and dyeing aid in industrial production. Ingestion of a certain amount of formaldehyde may cause corrosive burns in the mouth, throat, and digestive tract. Therefore, it is very necessary to use simple and effective detection methods to ensure human health and food safety. Herein, a novel fluorescent probe NFD based on naphthalimide for the detection of formaldehyde in food was designed and synthesized. The probe had a remarkable fluorescence response to formaldehyde at 554 nm. And it exhibited fascinating advantages of good selectivity, high sensitivity, and low detection limit. In addition, the solid sensor prepared by loading the probe on the filter paper was successfully realized the visual detection of liquid and gaseous formaldehyde. More importantly, the probe possessed excellent stability in the detection of formaldehyde in real food samples and animal serum samples.

2.
Heliyon ; 9(1): e12968, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36647394

RESUMO

Corona Virus Disease 2019 (COVID-19) is presently the largest international public health event, individuals infected by the virus not only have symptoms such as fever, dry cough, and lung infection at the time of onset, but also possibly have sequelae in the cardiovascular system, respiratory system, nervous system, mental health and other aspects. However, numerous studies have depicted that the active ingredients in tea show good antiviral effects and can treat various diseases by regulating multiple pathways, and the therapeutic effects are associated with the categories of chemical components in tea. In this review, the differences in the content of key active ingredients in different types of tea are summarized. In addition, we also highlighted their effects on COVID-19 and connected sequelae, further demonstrating the possibility of developing a formulation for the prevention and treatment of COVID-19 and its sequelae through tea extracts. We have a tendency to suggest forestalling and treating COVID-19 and its sequelae through scientific tea drinking.

3.
Environ Pollut ; 319: 121005, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36608731

RESUMO

The organic modification of three-dimensional porous diatom frustules (biosilica) and their fossils (diatomite) is promising in heavy metal adsorption. However, the preparation of such materials involves complex processes, high costs, and environmental hazards. In this study, organic-biosilica composites based on in situ self-modification of diatoms were prepared by freeze-drying pretreatment. Freeze-drying resulted in the release of the intercellular organic components of diatoms, followed by loading on the surface of their diatom frustules. The bio-adsorbent exhibits outstanding Cd2+ adsorption capacity (up to 220.3 mg/g). The adsorption isotherms fitted the Langmuir model and the maximum adsorption capacity was 4 times greater than that of diatom biosilica (54.1 mg/g). The adsorption kinetics of Cd2+ was adequately described by a pseudo-second-order model and reached equilibrium within 30 min. By combining focused ion beam thinning with transmission electron microscopy-energy dispersive X-ray spectroscopy, the internal structure of the composite and the Cd2+ distribution were investigated. The results showed that the organic matter of the composite adsorbed approximately 10 times more Cd2+ than inorganic biosilica. The adsorption mechanism was dominated by complexation between the abundant organic functional groups (amide, carboxyl, and amino groups) on the surfaces of composite and Cd2+. The bio-adsorbent was demonstrated to have wide applicability in the presence of competitive cations (Na+, K+, Ca2+, and Mg2+) and under a wide range of pH (3-10) conditions. Thus, the self-modification of diatoms offers a promising organic-inorganic composite for heavy metal remediation.


Assuntos
Diatomáceas , Metais Pesados , Poluentes Químicos da Água , Diatomáceas/química , Cádmio , Microscopia Eletrônica de Transmissão , Cátions , Adsorção , Cinética , Concentração de Íons de Hidrogênio
4.
Food Chem ; 409: 135272, 2023 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-36623357

RESUMO

Amino-modified Zn/Fe bimetallic metal-organic frameworks (NH2-Zn/Fe-MIL-88) were synthesized using a one-step solvothermal method with FeCl3·6H2O and Zn(NO3)2·6H2O as metal salts and 2-aminoterephthalic acid as organic ligand. The morphology of NH2-Zn/Fe-MIL-88 can be regulated from octahedral-like to spindle-like with changing molar ratios of metal salts. Using NH2-Zn/Fe-MIL-88 as sorbent, a dispersive solid-phase extraction with putting sorbents into sample solution to extract targets was developed to preconcentrate phytohormones in vegetables. To study the extraction efficiency, a series of NH2-Zn/Fe-MIL-88s with varying molar ratios of metal salts were prepared. The results indicated that NH2-Zn/Fe-MIL-88(1) presented the highest extraction efficiency (82.6 %-98.1 %) to phytohormones among all prepared NH2-Zn/Fe-MIL-88(x). The limits of detection were calculated at 0.07-0.15 ng/mL. The adsorption isotherms and kinetic parameters of NH2-Zn/Fe-MIL-88 for phytohormones were conformed to Langmuir and pseudo-second-order models. The NH2-Zn/Fe-MIL-88 as sorbent combined with HPLC was applied to detect phytohormones in cucumber and tomato samples.


Assuntos
Estruturas Metalorgânicas , Verduras , Sais , Reguladores de Crescimento de Plantas , Extração em Fase Sólida , Zinco
5.
Clin Transl Med ; 13(1): e1171, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36639826

RESUMO

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes coronavirus disease 2019 (COVID-19), which is still devastating economies and communities globally. The increasing infections of variants of concern (VOCs) in vaccinated population have raised concerns about the effectiveness of current vaccines. Patients with autoimmune diseases (PAD) under immunosuppressant treatments are facing higher risk of infection and potentially lower immune responses to SARS-CoV-2 vaccination. METHODS: Blood samples were collected from PAD or healthy controls (HC) who finished two or three doses of inactivated vaccines. Spike peptides derived from wild-type strain, delta, omicron BA.1 were utilised to evaluate T cell responses and their cross-recognition of delta and omicron in HC and PAD by flow cytometry and ex vivo IFNγ-ELISpot. RESULTS: We found that inactivated vaccine-induced spike-specific memory T cells were long-lasting in both PAD and HC. These spike-specific T cells were highly conserved and cross-recognized delta and omicron. Moreover, a third inactivated vaccine expanded spike-specific T cells that responded to delta and omicron spike peptides substantially in both PAD and HC. Importantly, the polyfunctionality of spike-specific memory T cells was preserved in terms of cytokine and cytotoxic responses. Although the extent of T cell responses was lower in PAD after two-dose, T cell responses were boosted to a greater magnitude in PAD by the third dose, bringing comparable spike-specific T cell immunity after the third dose. CONCLUSION: Inactivated vaccine-induced spike-specific T cells remain largely intact against delta and omicron variants. This study expands our understanding of inactivated vaccine-induced T cell responses in PAD and HC, which could have important indications for vaccination strategy.


Assuntos
Doenças Autoimunes , Vacinas contra COVID-19 , COVID-19 , Linfócitos T , Humanos , Doenças Autoimunes/imunologia , COVID-19/imunologia , COVID-19/prevenção & controle , Vacinas contra COVID-19/imunologia , SARS-CoV-2 , Linfócitos T/imunologia , Vacinas de Produtos Inativados
6.
J Pharm Pharmacol ; 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36656101

RESUMO

OBJECTIVES: Gefitinib is mainly used for the treatment of non-small-cell lung cancer. Hepatotoxicity is one of the main side effects of gefitinib, and seriously affects the treatment process of the disease. However, the hepatotoxicity mechanism of gefitinib remains unclear. METHODS: The hepatotoxicity of different doses of gefitinib was investigated in mice and AML-12 cells, and the possible correlation of hepatotoxicity with CYP450 was analysed. KEY FINDINGS: The toxic effects of gefitinib were confirmed by the increased liver index, decreased body weight and survival rate, injured liver function and histopathology followed 16 days of oral administration. Gefitinib (400 mg/kg) upregulated the hepatic mRNA expression of CYP1A1 and downregulated the CYP2D9 and CYP2D10 in mice. Furthermore, we verified that gefitinib produced cytotoxicity on AML-12 cells in a dose and time-dependent manner, and confirmed that gefitinib (20 µM) induced cell apoptosis, upregulated mRNA expression of CYP1A1 and downregulated CYP2D9 and CYP2D10. Pearson correlation analysis also showed that the hepatotoxicity of gefitinib was positively correlated with CYP1A1 and negatively correlated with CYP2D9 and CYP2D10. CONCLUSIONS: Our results suggested that the hepatotoxicity gefitinib may be associated with CYP1A1, CYP2D9 and CYP2D10. These findings will contribute to a better understanding of the mechanism of gefitinib hepatotoxicity.

7.
Chempluschem ; 88(1): e202200413, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36680306

RESUMO

A series of gallium(III) amide corroles including meso-5,15-bis(pentafluorophenyl)-10-(4-Pyridinamide-phenyl)corrole gallium (III) (1-Ga), meso-5,15-bis(pentafluorophenyl)-10-(4-Furamide-phenyl)corrole gallium(III) (2-Ga) and meso-5,15-bis(pentafluorophenyl)-10-(4-Thiophenamide-phenyl)corrole gallium(III) (3-Ga) were synthesized. The interaction of these complexes with DNA and their photodynamic antitumor activities have been studied. UV spectra titration showed that these gallium(III) corroles interact with calf thymus DNA (CT-DNA) through an external binding mode. All three gallium(III) corroles can effectively generate singlet oxygen under illumination and have good photostability. Among the three gallium(III) corroles, 2-Ga exhibited excellent photodynamic antitumor activity against the tested tumor cell lines under light irradiation (625±2 nm, 0.3 mW/cm2 , 1.08 J/cm2 ). The best phototoxicity was observed by 2-Ga against HepG2 cells (IC50 =6.3±0.9), which is even better than temoporfin (IC50 =8.4±1.8). It could block HepG2 cells in the sub-G0 phase and effectively induce apoptosis of HepG2 cells under 625 nm light irradiation.


Assuntos
Gálio , Neoplasias , Porfirinas , Gálio/farmacologia , Gálio/química , Porfirinas/química , DNA/química , Linhagem Celular Tumoral
8.
Int J Environ Health Res ; : 1-13, 2023 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-36682058

RESUMO

This study aimed to explore the association between household solid cooking fuel use and dementia prevalence and the mediating effect of depression on this association. A total of 3404 (2018) and 1379 (2015 to 2018) older participants (≥65) from the China Health and Retirement Longitudinal Study (CHARLS) were enrolled in the cross-sectional and retrospective longitudinal analyses, respectively. The results showed that solid cooking fuel use was associated with an increased dementia prevalence (adjusted OR = 1.44) from 2015 to 2018. The indirect effect of depression on this association explained 7.14% and 13.11% variances in the cross-sectional and longitudinal mediating model, respectively. Thus, household solid cooking fuel use is a risk factor for the development of dementia, and depressive symptoms partially accounted for this association. The use of improved cookstoves and clean fuel in households and air cleaners and early intervention in depression may reduce the incidence of dementia.

9.
ACS Synth Biol ; 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36653175

RESUMO

The UAG-based genetic code expansion (GCE) enables site-specific incorporation of noncanonical amino acids (ncAAs) harboring novel chemical functionalities in specific target proteins. However, most GCE studies were done in several whole-genome engineered chassis cells whose hundreds of UAG stop codons were systematically edited to UAA to avoid readthrough in protein synthesis in the presence of GCE. The huge workload of removing all UAG limited the application of GCE in other microbial cell factories (MCF) such as Bacillus subtilis, which has 607 genes ended with UAG among its 4245 coding genes. Although the 257 essential genes count only 6.1% of the genes in B. subtilis, they transcribe 12.2% of the mRNAs and express 52.1% of the proteins under the exponential phase. Here, we engineered a strain named Bs-22 in which all 22 engineerable UAG stop codons in essential genes were edited to UAA via CRISPR/Cas9-mediated multiple-site engineering to minimize the negative effect of GCE on the expression of essential genes. Besides the process of constructing GCE-compatible B. subtilis was systematically optimized. Compared with wild-type B. subtilis (Bs-WT), the fluorescence signal of the eGFP expression could enhance 2.25-fold in Bs-22, and the production of protein tsPurple containing l-(7-hydroxycoumarin-4-yl) ethylglycine (Cou) was increased 2.31-fold in Bs-22. We verified that all purified tsPurple proteins from Bs-22 contained Cou, indicating the excellent fidelity of the strategy. This proof-of-concept study reported efficient overexpression of ncAA-rich proteins in MCF with minimized engineering, shedding new light on solving the trade-off between efficiency and workload.

10.
Int J Biol Macromol ; 230: 123202, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36639076

RESUMO

Divalent metal ions such as magnesium (Mg2+), manganese (Mn2+), and zinc (Zn2+) play important roles in regulating innate immune responses. Lipopolysaccharide stimulation led to increased intracellular Mn and Zn in macrophages. However, the effect of those metal ions in regulating lipopolysaccharide-induced innate immune responses remains unclear. Here, we uncovered that both Mn2+ and Zn2+ have immunostimulatory effects, which could potentiate the lipopolysaccharide-induced expression of interferon-stimulated genes (ISGs), cytokines and pro-inflammatory genes in a dose-dependent manner. Enhancement of lipopolysaccharide-induced innate immune gene expression by Mn2+ varies between 10 % and 900 %. Conversely, the chelating of Mn2+ almost totally diminished Mn2+-enhanced lipopolysaccharide-induced gene expression. In addition, Mn2+ exerted its ability to potentiate LPS-induced innate immune gene expression regardless of slight pH changes. Importantly, we found that Mn2+ potentiates lipopolysaccharide-induced immune responses independent of TLR4 but partially relies on cGAS-STING pathway. Further in vivo study showed that colloidal Mn2+ salt (Mn jelly [MnJ]) pretreatment exacerbated lipopolysaccharide-induced septic shock and mice death. In conclusion, we demonstrated that Mn2+ plays an essential role in boosting lipopolysaccharide-induced innate immune responses. These findings greatly expand the current understanding of the immunomodulatory potential of divalent metal Mn2+ and may provide a potential therapeutic target to prevent excessive immune responses.

11.
Oxid Med Cell Longev ; 2023: 1117431, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36644579

RESUMO

Colon cancer is a relatively common malignant tumor of the digestive tract. Currently, most colon cancers originate from adenoma carcinogenesis. By screening various licorice flavonoids with anticancer effects, we found that glabridin (GBN) has a prominent anticolon cancer effect. First, we initially explored whether GBN can inhibit proliferation, migration, and invasion and induce apoptosis in SW480 and SW620 cells. Next, we exploited reverse virtual and proteomics technologies to screen out closely related target pathways on the basis of a drug and target database. At the same time, we constructed the structure of the GBN target pathway in colon cancer. We predicted that GBN can regulate the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT)-mammalian target of the rapamycin pathway (mTOR) pathway to fight colon cancer. Finally, through Western blot analysis and qRT-PCR, we verified that the expression levels of the PI3K, AKT, and mTOR proteins and genes in this pathway were significantly reduced after GBN administration. In short, the promising discovery of the anticolon cancer mechanism of GBN provides a reliable experimental basis for subsequent new drug development.


Assuntos
Neoplasias do Colo , Proteínas Proto-Oncogênicas c-akt , Humanos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais , Fosfatidilinositol 3-Quinases/metabolismo , Proteômica , Linhagem Celular Tumoral , Proliferação de Células , Detecção Precoce de Câncer , Serina-Treonina Quinases TOR/metabolismo , Fosfatidilinositol 3-Quinase/metabolismo , Neoplasias do Colo/genética , Apoptose
12.
Int J Cancer ; 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36594582

RESUMO

To reduce the risk of relapse after allogeneic hematopoietic stem cell transplantation (allo-HSCT), there have been continuing efforts to optimize the conditioning regimens. Our study aimed to analyze the risk factors associated with the relapse of relapsed/refractory (R/R), high-risk acute myeloid leukemia (AML) and high-risk myelodysplastic syndrome (MDS) post-transplant and the efficacy of a new conditioning regimen involving decitabine and cladribine. Clinical data of 125 patients with R/R AML, high-risk AML and high-risk MDS who underwent allo-HSCT were collected. In addition, 35 patients with R/R AML, high-risk AML and high-risk MDS received treatment with a new conditioning regimen including decitabine and cladribine. Cox regression analysis was used to identify risk factors associated with OS, RFS and relapse. Among 125 patients who underwent allo-HSCT, CR before allo-HSCT and matched sibling donors were independent protective factors for OS. DNMT3A abnormality was an independent risk factor for both relapse and RFS. Among 35 patients who received a new conditioning regimen containing decitabine and cladribine, only six patients relapsed and 1-year cumulative incidence of relapse was 11.7%. Moreover, this new regimen showed efficient MRD clearance early after allo-HSCT. The combined decitabine- and cladribine-based conditioning regimen showed a low relapse rate and a high survival without an increased incidence of GVHD or adverse effects and thus has potential for use in allo-HSCT for R/R AML, high-risk AML and high-risk MDS.

13.
J Agric Food Chem ; 71(1): 96-109, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36541706

RESUMO

Cancer is the most serious problem for public health. Traditional treatments often come with unavoidable side effects. Therefore, the therapeutic effects of natural products with wide sources and low toxicity are attracting more and more attention. Polysaccharides have been shown to have cancer-fighting potential, but the molecular mechanisms remain unclear. The mammalian target of rapamycin (mTOR) pathway has become an attractive target of antitumor therapy research in recent years. The regulation of mTOR pathway not only affects cell proliferation and growth but also has an important effect in tumor metabolism. Recent studies indicate that dietary polysaccharides play a vital role in cancer prevention and treatment by regulating mTOR pathway. Here, the progress in targeting mTOR signaling by dietary polysaccharides in cancer prevention and their molecular mechanisms are systemically summarized. It will promote the understanding of the anticancer effects of polysaccharides and provide reference to investigators of this cutting edge field.


Assuntos
Neoplasias , Polissacarídeos , Humanos , Polissacarídeos/farmacologia , Polissacarídeos/uso terapêutico , Serina-Treonina Quinases TOR/genética , Serina-Treonina Quinases TOR/metabolismo , Transdução de Sinais , Neoplasias/metabolismo , Sirolimo/farmacologia , Sirolimo/uso terapêutico
14.
Chemosphere ; 313: 137508, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36493889

RESUMO

The biotransformation of sulfamonomethoxine (SMM) was studied in an aerobic granular sludge (AGS) system to understand the role of sorption by microbial cells and extracellular polymeric substances (EPS) and the role of functional microbe/enzyme biodegradation. Biodegradation played a more important role than adsorption, while microbial cells covered with tightly bound EPS (TB-EPS) showed higher adsorption capacity than microbial cells themselves or microbial cells covered with both loosely bound EPS (LB-EPS) and TB-EPS. The binding tests between EPS and SMM and the spectroscopic analyses (3D-EEM, UV-Vis, and FTIR) were performed to obtain more information about the adsorption process. The data showed that SMM could interact with EPS by combining with aromatic protein compounds, fulvic acid-like substances, protein amide II, and nucleic acids. Batch tests with various substances showed that SMM removal rates were in an order of NH2OH (60.43 ± 2.21 µg/g SS) > NH4Cl (52.96 ± 0.30 µg/g SS) > NaNO3 (31.88 ± 1.20 µg/g SS) > NaNO2 (21.80 ± 0.42 µg/g SS). Hydroxylamine and hydroxylamine oxidoreductase (HAO) favored SMM biotransformation and the hydroxylamine-mediated biotransformation of SMM was more effective than others. In addition, both ammonia monooxygenase (AMO) and CYP450 were able to co-metabolize SMM. Analysis of UPLC-QTOF-MS indicated the biotransformation mechanisms, revealing that acetylation of arylamine, glucuronidation of sulfonamide, deamination, SO2 extrusion, and δ cleavage were the five major transformation pathways. The detection of TP202 in the hydroxylamine-fed Group C indicated a new biotransformation pathway through HAO. This study contributes to a better understanding of the biotransformation of SMM.


Assuntos
Esgotos , Sulfamonometoxina , Esgotos/química , Análise Espectral , Biotransformação , Hidroxilaminas
15.
Int Immunopharmacol ; 113(Pt A): 109417, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36461606

RESUMO

AIM: To determine the neuroprotective effects of fluoxetine on depression-like and motor behaviors in rats treated with lipopolysaccharide (LPS) and the mechanisms involved. METHODS: A rat model of depression in Parkinson's disease (dPD) was established by administering LPS (0.5 mg/kg, i.p.) for 4 days. The sucrose preference test (SPT), open field test (OFT), and rotarod test evaluated depression-like and motor behaviors. White matter fiber integrity and intrinsic activity in the brain were assessed using magnetic resonance imaging. For pathological and molecular expression detection, hematoxylin-eosin staining, immunohistochemistry, Luminex technology, western blotting, and quantitative real-time PCR were used. RESULTS: Fluoxetine increased the sucrose preference in the SPT, the horizontal and center distances in the OFT, and the standing time in the rotarod test. Fluoxetine also improved intrinsic activities and white matter fiber damage in the brain, increased c-Fos expression, reduced Iba-1 expression in the prefrontal cortex, hippocampus, and substantia nigra, and increased TH expression in the substantia nigra. Fluoxetine reduced the concentration of inflammatory cytokines (IL-1α, IL-6, TNF-α, and IFN-γ). The gene and protein expression of Notch1, Jagged1, Hes1, and Hes5 were significantly lower than the LPS group after treatment with fluoxetine. CONCLUSION: Fluoxetine plays neuroprotective effects in relieving LPS-induced depression-like and motor behaviors. The underlying mechanisms may be related to inhibiting microglial activation, regulating the Notch signaling pathway, and inhibiting the inflammatory response.


Assuntos
Lipopolissacarídeos , Fármacos Neuroprotetores , Animais , Ratos , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/uso terapêutico , Fluoxetina/uso terapêutico , Doenças Neuroinflamatórias , Sacarose , Transdução de Sinais
16.
J Chromatogr Sci ; 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36477207

RESUMO

A high-performance liquid chromatograph with diode array detector was established for the simultaneous determination of five phenylethanoid glycosides in Syringa pubescens Turcz. The optimal chromatographic conditions were achieved on a Zorbax C18 column using gradient elution with 0.5% aqueous acetic acid and acetonitrile as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was developed as follows: 0-10 min, 276 nm; 10-45 min, 332 nm. The validation of the method including linearity, precision, stability, accuracy, repeatability and recovery was tested. The chemometric analysis including hierarchical cluster analysis and principal component analysis was employed to investigate the similarity and difference of samples from different geographical origin. The results revealed that S. pubescens samples were divided into four clusters based on the phenylethanoid glycosides contents. Antioxidant activity of extract was measured using three different methods including α,α-diphenyl-ß-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical scavenging assays, and ferric reducing antioxidant power assay. Furthermore, different phenylethanoid glycosides exhibited different contribution to antioxidant capacities. This study provides a foundation for the quality evaluation and offers scientific data for the utilization of S. pubescens resources.

17.
Cartilage ; : 19476035221144745, 2022 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-36541677

RESUMO

OBJECTIVE: The current study aims to investigate the factors that could predict response to intra-articular corticosteroid injection (IACI) in patients with knee osteoarthritis (KOA). METHODS: Data of participants were retrieved from the Osteoarthritis Initiative database. Participants with at least one IACI treatment on single or bilateral knees within the first 5 years of follow-up were retrospectively included. Demographic data, clinical and radiographic variables were collected at both baseline and the first follow-up after IACI treatment. Positive response to IACI treatment was defined as >20% reduction of Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain score from V0 to V1. All the variables with P < 0.2 after the comparison between the response and non-response groups were included in a multivariable logistic regression model to identify independent response predictive patient-specific valuables. Receiver operating characteristic curves were performed to establish the cutoff values of independent predictors. RESULTS: The current study included a total of 385 participants (473 knees), with 155 and 318 knees classified into the response group and non-response group, respectively. Those with satisfied responses to IACI treatment had significantly higher WOMAC pain score (P < 0.001), disability score (P = 0.002), and stiffness score (P = 0.015) at the baseline. Baseline WOMAC pain score showed significant association with positive response to IACI treatment in multivariate logistic analysis and the best cutoff value was 5 points. The rate of analgesics utilization was lower (P = 0.014) in the response group than the non-response group after the IACI treatment. CONCLUSION: KOA patients with a baseline WOMAC pain score ≥5 are more likely to benefit from IACI treatment.

18.
Adv Sci (Weinh) ; : e2205411, 2022 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-36574468

RESUMO

Large-area polymer light-emitting diodes (PLEDs) manufactured by printing are required for flat-panel lighting and displays. Nevertheless, it remains challenging to fabricate large-area and stable deep-blue PLEDs with narrowband emission due to the difficulties in precisely tuning film uniformity and obtaining single-exciton emission. Herein, efficient and stable large-area deep-blue PLEDs with narrowband emission are prepared from encapsulated polydiarylfluorene. Encapsulated polydiarylfluorenes presented an efficient and stable deep-blue emission (peak: 439 nm; full width at half maximum (FWHM): 39 nm) in the solid state due to their single-chain emission behavior without inter-backbone chain aggregation. Large-area uniform blade-coated films (16 cm2 ) are also fabricated with excellent smoothness and morphology. Benefitting from efficient emission and excellent printed capacity, the blade-coated PLEDs with a device area of 9 mm2 realized uniform deep-blue emission (FWHM: 38 nm; CIE: 0.153, 0.067), with a corresponding maximum external quantum efficiency and the brightness comparable to those of devices based on spin-coated films. Finally, considering the essential role of deep-blue LEDs, a preliminary patterned PLED array with a pixel size of 800 × 1000 µm2 and a monochrome display is fabricated, highlighting potential full-color display applications.

19.
Int J Mol Sci ; 23(24)2022 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-36555215

RESUMO

Endometrial decidualization plays a pivotal role during early pregnancy. Compromised decidualization has been tightly associated with recurrent implantation failure (RIF). Primary cilium is an antenna-like sensory organelle and acts as a signaling nexus to mediate Hh, Wnt, TGFß, BMP, FGF, and Notch signaling. However, whether primary cilium is involved in human decidualization is still unknown. In this study, we found that primary cilia are present in human endometrial stromal cells. The ciliogenesis and cilia length are increased by progesterone during in vitro and in vivo decidualization. Primary cilia are abnormal in the endometrium of RIF patients. Based on data from both assembly and disassembly of primary cilia, it has been determined that primary cilium is essential to human decidualization. Trichoplein (TCHP)-Aurora A signaling mediates cilia disassembly during human in vitro decidualization. Mechanistically, primary cilium modulates human decidualization through PTEN-PI3K-AKT-FOXO1 signaling. Our study highlights primary cilium as a novel decidualization-related signaling pathway.


Assuntos
Cílios , Proteínas Proto-Oncogênicas c-akt , Gravidez , Feminino , Humanos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Cílios/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Endométrio/metabolismo , Transdução de Sinais , Células Estromais/metabolismo , Decídua/metabolismo
20.
Artigo em Inglês | MEDLINE | ID: mdl-36554588

RESUMO

Older adults with subjective cognitive decline are at increased risk of future pathological cognitive decline and dementia. Subjective memory decline is an early sign of cognitive decline; preventing or slowing cognitive decline in at-risk populations remains an elusive issue. This study aimed to examine the cognitive trajectories and factors in older adults with subjective memory decline. Latent growth curve models (LGCMs) were fitted to examine the cognitive function trajectories and factors among 1465 older adults (aged 60+ years) with subjective memory decline. Data were obtained from four waves from the China Health and Retirement Longitudinal Study (CHARLS, 2011-2018), which is a large nationally representative sample of the Chinese population. The results showed that older adults with better initial cognition had a slower decline rate, which may be accelerated by advanced age, low-level education, a rapid decrease in instrumental activities of daily living (IADL) ability, and rapid increase in depression levels. This study was the first to examine the trajectories of cognitive function and its factors in a high-risk population with subjective memory decline. These findings may guide prevention approaches to tackle the issues of cognitive function decline and dementia.


Assuntos
Disfunção Cognitiva , Demência , Humanos , Idoso , Estudos Longitudinais , Atividades Cotidianas , Disfunção Cognitiva/epidemiologia , Disfunção Cognitiva/psicologia , Cognição , China/epidemiologia , Demência/epidemiologia , Transtornos da Memória/epidemiologia
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