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1.
Interv Neuroradiol ; : 15910199221097264, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35505598

RESUMO

OBJECTIVE: The authors sought to verify the use of a preoperative simulation software for the treatment of intracranial aneurysms using flow diverters (FDs) based on three-dimensional rotational angiography (3DRA) data. METHODS: Based on 3DRA data, the preoperative simulation software (UKNOW) was used to simulate the deployment of virtual FDs. The length and dimensions of virtual and real devices were compared. The deployment plan recommended by the UKNOW software was preliminarily used to complete implantations in the real world. During the experiment, experienced neurointerventional experts were responsible for supervising and judging information such as the length, dimension, and deployment location of the FDs. RESULTS: This study retrospectively analyzed the data of 29 patients who received FD treatment. There was no statistical difference between the length of the real device and the virtual device (p = 0.6). The dimensions of FDs recommended by the software were consistent with the dimensions used in 24 out of the 29 real cases. In four of the remaining five cases, neurointerventional experts found that the FD dimensions recommended by the software were superior to those were actually used. Thus, the accuracy rate for FD dimension recommendations by the UKNOW software was 96.6% (28/29). Procedures performed in five cases using deployment plans recommended by the UKNOW software all achieved good postoperative results; the deployment positions of the device were reasonable, and all devices showed good wall adherence. CONCLUSIONS: UKNOW software could accurately simulate the length and deployment position of the real FDs and provide suitable device dimensions.

2.
J Colloid Interface Sci ; 622: 625-636, 2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35533478

RESUMO

Carbonaceous-magnetic composites are the most appealing candidates for electromagnetic wave absorption, and creating hollow interiors and nanopores in the composites is commonly recognized as an essential strategy to reinforce their overall performances. Herein, we propose a spatial confinement strategy mediated by Co2(OH)2CO3 nanosheet assemblies for achieving highly dispersed Co nanoparticles into hollow porous N-doped carbon shells (HP-Co@NCS). Systematic multi-technique characterizations indicate that the Co2(OH)2CO3 nanosheet assemblies simultaneously play a trifunctional role during the synthesis, including Co source, template of the hollow interior cavities, and micro-/mesopore porogen. The chemical composition can be modulated by simply varying the ratio of Co2(OH)2CO3 and carbon source (dopamine). The optimized HP-Co@NCS absorber exhibits a well-defined hollow structure and unprecedented high porosity (specific surface area of 742 m2 g-1) even with a high metallic Co content of 35.8 wt%. These profitable structural characteristics can facilitate incident EM waves penetrating the absorber's interior and promoting multiple reflections and scattering. Therefore, the HP-Co@NCS absorber exhibits efficient microwave absorption ability with a minimum reflection loss of -39.0 dB at a thin thickness of 2.5 mm and an effective absorption bandwidth up to 5.5 GHz (12.5-18.0 GHz) at a thin thickness of 2.0 mm. This work provides a new methodology to design advanced carbonaceous-magnetic composite materials with hollow porous structures for microwave absorption.

3.
Echocardiography ; 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35505628

RESUMO

OBJECTIVE: Echocardiography is a time and cost-effective imaging modality, providing evidence of myocardial ischemia by detecting the regional wall motion abnormalities (RWMA). However, quite a few coronary heart disease (CHD) patients do not present RWMA. The left atrium (LA) plays an irreplaceable role in determining the prognosis and risk stratification of cardiovascular disease including CHD. In this present study, we intend to explore the myocardial mechanics changes of LA mainly using four-dimensional (4D) LA quantitative volume-strain in CHD patients without RWMA at rest but were confirmed by coronary angiography (CAG) and to figure out several variables of the LA that could contribute to the identification of those patients. METHODS: We prospectively enrolled 76 patients who underwent two-dimensional echocardiography (2DE), four-dimensional echocardiography (4DE), and CAG for suspected CHD but without echocardiographic visible RWMA at rest. Patients diagnosed with CHD by CAG were furtherly divided into three groups according to the extent of coronary stenosis accessed by Gensini score (GS) as the mild, moderate, and severe CHD group. Twenty-four subjects with negative CAG results served as the control group. LA end-systolic anteroposterior diameter (LAAPD) and biplane LV ejection fraction (Biplane LVEF) were measured by 2DE; LA maximum volume (LAVmax), LA minimum volume (LAVmin), LA volume at the onset of atrial contraction (LAVpreA), LAVmax index (LAVmaxI), LA ejection volume (LAEV), LA ejection fraction (LAEF) accompanied by LA longitudinal strain during reservoir phase (LASr), conduit phase (LAScd), contraction phase (LASct) and LA circumferential strain during reservoir phase (LASr_c), conduit phase (LAScd_c), contraction phase (LASct_c) were measured by 4DE automatically. We compared these parameters between groups, explored how they change and whether they are related to the CHD severity. RESULTS: LAEF, LASr_c, and LASct_c was lower in CHD group compared with the control group (p = .031, .002, .004, respectively). Pearson correlation analysis showed that LASr, LASct, LASr_c, and LASct_c negatively correlated with the GS. Additionally, LASr of patients in the severe CHD group decreased significantly compared with those in the mild CHD group, moderate CHD group, and control group, demonstrating the highest area under the receiver operating characteristic (ROC) curve (AUC) (AUC = .736 [p = .003, 95% CI .589-.884], sensitivity 67.8%, specificity 70.6%) with the cut-off value of 17.5% for predicting severe CHD patients. CONCLUSION: Four-dimensional LA strain may provide new insight into identification and management for CHD patients and correlate with CHD severity. LASr showed good sensitivity (67.8%) and specificity (70.6%) for diagnosing severe CHD individuals.

4.
Cancers (Basel) ; 14(9)2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35565395

RESUMO

Cell-based immunotherapy, such as chimeric antigen receptor (CAR) T cell therapy, has revolutionized the treatment of hematological malignancies, especially in patients who are refractory to other therapies. However, there are critical obstacles that hinder the widespread clinical applications of current autologous therapies, such as high cost, challenging large-scale manufacturing, and inaccessibility to the therapy for lymphopenia patients. Therefore, it is in great demand to generate the universal off-the-shelf cell products with significant scalability. Human induced pluripotent stem cells (iPSCs) provide an "unlimited supply" for cell therapy because of their unique self-renewal properties and the capacity to be genetically engineered. iPSCs can be differentiated into different immune cells, such as T cells, natural killer (NK) cells, invariant natural killer T (iNKT) cells, gamma delta T (γδ T), mucosal-associated invariant T (MAIT) cells, and macrophages (Mφs). In this review, we describe iPSC-based allogeneic cell therapy, the different culture methods of generating iPSC-derived immune cells (e.g., iPSC-T, iPSC-NK, iPSC-iNKT, iPSC-γδT, iPSC-MAIT and iPSC-Mφ), as well as the recent advances in iPSC-T and iPSC-NK cell therapies, particularly in combinations with CAR-engineering. We also discuss the current challenges and the future perspectives in this field towards the foreseeable applications of iPSC-based immune therapy.

5.
Front Oncol ; 12: 824541, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35530339

RESUMO

Background: The aim of this study was to derive and validate a decision tree model to predict disease-specific survival after curative resection for primary cholangiocarcinoma (CCA). Method: Twenty-one clinical characteristics were collected from 482 patients after curative resection for primary CCA. A total of 289 patients were randomly allocated into a training cohort and 193 were randomly allocated into a validation cohort. We built three decision tree models based on 5, 12, and 21 variables, respectively. Area under curve (AUC), sensitivity, and specificity were used for comparison of the 0.5-, 1-, and 3-year decision tree models and regression models. AUC and decision curve analysis (DCA) were used to determine the predictive performances of the 0.5-, 1-, and 3-year decision tree models and AJCC TNM stage models. Results: According to the fitting degree and the computational cost, the decision tree model derived from 12 variables displayed superior predictive efficacy to the other two models, with an accuracy of 0.938 in the training cohort and 0.751 in the validation cohort. Maximum tumor size, resection margin, lymph node status, histological differentiation, TB level, ALBI, AKP, AAPR, ALT, γ-GT, CA19-9, and Child-Pugh grade were involved in the model. The performances of 0.5-, 1-, and 3-year decision tree models were better than those of conventional models and AJCC TNM stage models. Conclusion: We developed a decision tree model to predict outcomes for CCA undergoing curative resection. The present decision tree model outperformed other clinical models, facilitating individual decision-making of adjuvant therapy after curative resection.

6.
Cell Tissue Res ; 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35543755

RESUMO

Tooth loss and maxillofacial bone defect are common diseases, which seriously affect people's health. Effective tooth and maxillofacial bone tissue regeneration is a key problem that need to be solved. In the present study, we investigate the role of PRMT6 in osteo/odontogenic differentiation and migration capacity by using SCAPs. Our results showed that knockdown of PRMT6 promoted the osteo/odontogenic differentiation compared with the control group, as detected by alkaline phosphatase activity, alizarin red staining, and the indicators of osteo/odontogenic differentiation measured by Western blot. In addition, overexpression of PRMT6 inhibited the osteo/odontogenic differentiation potentials of SCAPs. Then, knockdown of PRMT6 promoted the migration ability and overexpression of PRMT6 inhibited the migration ability in SCAPs. Mechanically, we discovered that the depletion of PRMT6 promoted the expression of CXCL12 by decreasing H3R2 methylation in the promoter region of CXCL12. In addition, PRMT6 formed a protein complex with LMNA, a nuclear structural protein. Depletion of LMNA inhibited the osteo/odontogenic differentiation and CXCL12 expression and increased the intranucleus PRMT6 in SCAPs. To sum up, PRMT6 might inhibit the osteo/odontogenic differentiation and migration ability of SCAPs via inhibiting CXCL12. And LMNA might be a negative regulator of PRMT6. It is suggested that PRMT6 may be a key target for SCAP-mediated bone and tooth tissue regeneration.

7.
Adv Mater ; : e2201046, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35560664

RESUMO

Extreme environments represent numerous harsh environmental conditions, such as temperature, pressure, corrosion and radiation. The tolerance of applications in extreme environments exemplifies significant challenges to both materials and their structures. Given the superior mechanical strength, electrical conductivity, thermal stability and chemical stability of nanocarbon materials, such as carbon nanotubes (CNTs) and graphene, they have been widely investigated as base materials for extreme environmental applications and have shown numerous breakthroughs in the fields of wide-temperature structural-material construction, low-temperature energy storage, underwater sensing and electronics operated at high temperatures. This review will examine the critical aspects of structural design and fabrication of nanocarbon materials for extreme environments, which includes the description of the underlying mechanism supporting the performance of nanocarbon materials against extreme environments, the principles of structural design of nanocarbon materials for the optimization of extreme environmental performances, and the fabrication processes developed for the realization of specific extreme environmental applications. Finally, perspectives on how CNTs and graphene can further contribute to the development of extreme environmental applications are presented. This article is protected by copyright. All rights reserved.

8.
Carbohydr Polym ; 288: 119348, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35450621

RESUMO

Solution plasma process (SPP) was employed to degrade chitosan. The effects of the initial pH value and the distance between the electrodes on the steady shear flow behavior, structural characterization, molecular conformation, and antioxidant activity of chitosan were investigated. The results revealed that a lower initial pH value and a narrower distance between the electrodes were beneficial to the decrease in viscosity and increase in shear-thinning capacity. Structural characterization of the chitosan by FT-IR and 1H NMR showed that chemical structure of chitosan was not destroyed at different process parameters. The results of XRD, HPSEC-MALLS, SEM, and AFM indicated that SPP degradation clearly decreased the crystallinity, molecular weight, molecular size, and molecular aggregation of chitosan. At initial pH values of 2.8 and 5.8, the molecular weight was 27.16 and 44.25 kDa, at the distance between the electrodes of 4 and 8 mm, it was 35.88 and 66.17 kDa, respectively. The results of DPPH and hydroxyl radical scavenging assays demonstrated that a lower initial pH value and a narrower distance between the electrodes enhanced the antioxidant activity.


Assuntos
Quitosana , Antioxidantes/química , Antioxidantes/farmacologia , Quitosana/química , Eletrodos , Concentração de Íons de Hidrogênio , Espectroscopia de Infravermelho com Transformada de Fourier
9.
Pharmaceutics ; 14(4)2022 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-35456543

RESUMO

In this study, the plasma pharmacokinetics and tissue disposition of sulfadiazine (SDZ) and its main metabolite, N4-acetyl sulfadiazine (ACT-SDZ), were compared between 18 and 24 °C following a single oral administration of SDZ at 50 mg/kg in grass carp (Ctenopharyngodon idella). The plasma and tissues were sampled from 0.167 h up to 96 h and analyzed by ultra-performance liquid chromatography with an ultraviolet detector. The pharmacokinetic parameters were estimated using a one-compartmental approach. Results showed that pharmacokinetics of SDZ and ACT-SDZ in plasma and tissues were notably influenced by the increase of temperature. The increased temperature shortened the absorption half-life (K01_HL) of SDZ and ACT-SDZ in gill, kidney, and plasma, but increased in liver and muscle + skin. The elimination half-life (K10_HF) and the area under concentration-time curve (AUC0-∞) of SDZ and ACT-SDZ all presented a declined trend. The apparent volume of distribution (V_F) of SDZ in plasma was increased from 0.93 to 1.64 L/kg, and the apparent systemic total body clearance (Cl_F) was also increased from 0.01 to 0.05 L/h/kg. Overall, the rise of temperature decreased K10_HF, AUC0-∞ of SDZ, and ACT-SDZ in plasma and tissues, but increased V_F and Cl_F in the plasma for SDZ.

10.
Pharmacotherapy ; 2022 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-35434830

RESUMO

STUDY OBJECTIVE: The objective of the present study was to examine the frequency distribution of five single-nucleotide polymorphisms (SNPs; rs1801394 A>G, rs1532268 C>T, rs162036 A>G, rs10380 C>T, and rs9332 C>T) of the methionine synthase reductase (MTRR) gene, their effects on methotrexate (MTX) concentration, and the risk of relapse in a Chinese pediatric population with acute lymphoblastic leukemia (ALL). DESIGN: This was a retrospective single-center study, and all analyses were exploratory. SETTING: Pediatric Department of Beijing Shijitan Hospital, Capital Medical University, Beijing, China. PATIENTS: One hundred and forty pediatric patients with ALL. INTERVENTION: All patients were treated according to the Chinese Children's Leukemia Group (CCLG)-ALL 2008 protocol. MEASUREMENTS AND MAIN RESULTS: Serum MTX concentrations were measured using fluorescence polarization immunoassay. Genotyping of five SNPs was performed using the Sequenom MassARRAY iPLEX platform. Chinese children with ALL had a significantly lower frequency of rs1801394 G than European (EUR) and South Asian (SAS) populations; significantly lower frequency of rs1532268 T than American (AMR), EUR, and SAS populations; and significantly lower frequencies of rs162036 G, rs10380 T, and rs9332 T than African and AMR populations (p < 0.01). Seven haplotypes were observed, with the ACACC being the most common haplotype (49.9%) in our study. The median dose-normalized concentrations of MTX in serum at 24 h in children with rs1532268 CT and TT genotypes were significantly higher than those with CC genotype (p = 0.04). Compared with children with AA-CC-AA-CC-CC diplotype, a significantly higher risk of relapse was observed in children with AG-CC-AA-CC-CC and AG-CC-AG-CC-CC diplotypes (p = 0.03 and 0.003, respectively). CONCLUSIONS: The present study confirmed the ethnic differences in the distribution of MTRR rs1801394, rs1532268, rs162036, rs10380, and rs9332 polymorphisms. The rs1532268 polymorphism had greater effects on MTX disposition. The AG-CC-AA-CC-CC and AG-CC-AG-CC-CC diplotypes were significantly associated with higher risk of relapse of ALL.

11.
Artigo em Inglês | MEDLINE | ID: mdl-35449809

RESUMO

Background: Naoluo Xintong decoction (NLXTD) is a traditional Chinese medicine (TCM) formula which has been used to improve neuronal functional recovery after cerebral ischemic stroke. However, the molecular mechanism underlying NLXTD's amelioration of ischemic stroke remains unclear. The present study was designed to explore the effect and mechanism of NLXTD on brain angiogenesis in a rat model with cerebral ischemia-reperfusion (I/R) injury targeting the hypoxia-inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) pathway. Materials and Methods: Cerebral I/R model was established by the classical middle cerebral artery occlusion (MCAO) method. Sprague-Dawley (SD) male rats (n = 80) were randomly divided into the sham-operation group, the model group, the HIF-1α inhibitor 2-methoxyestradiol (2ME2) group, the 2ME2 with NLXTD group, and the NLXTD group. Neurological deficit test, TTC staining, H&E staining, TUNEL staining, immunohistochemistry (IH), immunofluorescence (IF), western blot, and quantitative RT-PCR were performed to evaluate the effect of NLXTD after MCAO. Results: Administration of NLXTD significantly decreased neuron deficiency scores, reduced brain infarct volume, and lowered damaged and apoptotic cells after brain I/R injury in rats. Meanwhile, NLXTD had a protective effect on angiogenesis by increasing the MVD and the expressions of BrdU and CD34, which enhanced the number of endothelial cells in the ischemic penumbra brain. NLXTD treatment significantly raised the protein and mRNA levels of HIF-1α, VEGF, VEGFR2, and Notch1 compared with the model treatment. In contrast, a specific HIF-1α inhibitor, 2ME2, inhibited the improvement of neurological function and angiogenesis in NLXTD-induced rats with cerebral I/R injury, suggesting that NLXTD played a positive role in ischemic brain injury by activating the HIF-1α/VEGF signaling pathway. Conclusions: NLXTD exerts neuroprotection targeting angiogenesis by upregulating the HIF-1α/VEGF signaling pathway on cerebral I/R injury rats.

12.
MedComm (2020) ; 3(2): e117, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35415706

RESUMO

The Japanese encephalitis (JE) live-attenuated vaccine SA14-14-2 and the chimeric vaccine IMOJEV (JE-CV) are two kinds of vaccines available for use worldwide. JE-CV was previously known as ChimeriVax-JE, that consists of yellow fever vaccine 17D (YFV-17D) from which the structural genes (prM/E) have been replaced with those of SA14-14-2. This study aimed to investigate the neutralizing antibody, protection efficacy, and specific T-cell response elicited by both vaccines in mice. The neutralizing antibodies produced by JE-CV were slightly lower than those produced by SA14-14-2, but the protection conferred by JE-CV was considerably lower in the low vaccine dose immunization group. Furthermore, the JE-CV did not induce a specific T-cell response against JEV NS3, while it did induce a potent antigen-specific T-cell response against the viral backbone vaccine YFV. In conclusion, this study is the first detailed investigation of the cellular immune response to the two vaccines. Enzyme-linked immunospot (ELISPOT) and flow staining suggest a more potent specific T-cell response against the JEV antigen was elicited in mice immunized with SA14-14-2 but not JE-CV. Using heterologous flaviviruses as a live-attenuated vaccine backbone may unlikely generate an optimal T-cell response against the vaccine strain virus and might affect the protective efficacy.

13.
Sci Total Environ ; 834: 155373, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35460790

RESUMO

Chloroacetonitriles (CANs) are highly toxic nitrogenous disinfection by-products (N-DBPs), which frequently appear in water supply systems and have attracted widespread attention. UV/persulfate (PS) is an effective method to degrade CANs. Bromide (Br-) is widespread in aquatic environments and reacts with oxidative radicals to produce secondary reactive bromine species (RBS), which affects the degradation of CANs by UV/PS. It was found that the degradation of CANs was highly inhibited by Br-. The apparent first-order reaction rate constants of monochloroacetonitrile (MCAN), dichloroacetonitrile (DCAN) and trichloroacetonitrile (TCAN) decreased from 2.63 × 10-3, 2.00 × 10-3 and 8.66 × 10-4 s-1 to 2.58 × 10-4, 1.61 × 10-4 and 1.59 × 10-4 s-1, respectively after adding 20 µM of Br-. HO• was the main radicals contributing to the degradation of CANs when the concentration of Br- was less than 10 µM, compared with SO4•- and direct photolysis. When the concentration of Br- was up to 20 µM, the contributions of RBS accounted for 85.7%, 90.7% and 89.9% of the apparent degradation rate constants of CANs, respectively. During the reaction, about 65% of nitrogen atoms were transformed into NO3- by the CC bond cleavage and oxidation. The yields of Cl- by dechlorination reaction accounted for 83.5%, 71.0% and 41.2% of the chlorine contents in MCAN, DCAN and TCAN, respectively. It was verified that CANs react with free bromine (HOBr) to produce bromochloroacetonitrile (BCAN). DCAN and TCAN are hydrolyzed to produce corresponding haloacetamides (HAMs), which are further reacted with HOBr to produce bromodichloroacetic acid (BDCAA). Furthermore, the generation of bromate was also worth noting via the oxidation of Br- in the UV/PS system.

14.
J Ethnopharmacol ; 294: 115292, 2022 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-35447200

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: XianLing GuBao Capsule (XLGB) is often used to treat osteoarthritis (OA), osteoporosis, fractures, and other musculoskeleton disorders. However, the molecular mechanism of XLGB for treating OA is still unclear. AIM OF THE STUDY: This study set out to uncover the molecular mechanism underlying the treatment of osteoarthritis with XLGB. MATERIALS AND METHODS: Disease genes were obtained from CTD, DisGeNET, and GeneCards databases, and XLGB drug targets were obtained from ETCM and target genes predicted by XLGB metabolic components reported in the literature. Then we used the Venn diagram viewer to extract disease and drug intersection genes as potential therapeutic genes for Protein-protein interaction (PPI), GO terminology, and KEGG pathway analysis. Subsequently, we performed qRT-PCR, Western blot and histological analysis to validate the therapeutic effect of XLGB against OA and its molecular mechanism. RESULTS: A total of 1039 OA genes and 949 XLGB target genes were collected, and finally 188 potential therapeutic target genes were obtained. PPI network analysis indicated that the main target genes for XLGB to treat OA include Akt1, Mapk3, Il-6, Il-1ß, Ptgs2, Mmp9, etc. The results of KEGG and GO enrichment analysis suggested that XLGB may treat OA by anti-inflammatory and reducing extracellular matrix degradation. In vitro, XLGB down-regulated the expressions of Mmp3, Mmp9, Mmp12, Mmp13, Cox-2, Il-6, increased the expression of Collagen II and Sox9. Mechanistically, XLGB inhibits the activation of PI3K/AKT/NF-κB and MAPK pathways. Moreover, the results of animal experiments indicated that XLGB reduced cartilage destruction, bone resorption, and synovitis in osteoarthritic rats. CONCLUSIONS: XLGB has a protective effect against OA by suppressing PI3K/AKT/NF-κB and MAPK signaling. Our study provides a theoretical basis for XLGB in the treatment of osteoarthritis.

15.
Dev Comp Immunol ; 133: 104429, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35489421

RESUMO

C type-lectins constitute a large family of pattern recognition receptors, playing important roles in insect immune defenses. Thitarodes xiaojinensis larvae showed distinct immune features after Ophiocordyceps sinensis, Cordyceps militaris, or Beauveria bassiana infection. Based on transcriptome and immunoblot analysis, we found that immulectin-2 (IML2) was induced after T. xiaojinensis larvae were infected by C. militaris or B. bassiana but maintained at a low level after larvae injected with O. sinensis or Ringer's buffer. Recombinant IML2 (rIML2) could promote melanization, encapsulation, phagocytosis, and hemocyte aggregation in vitro. RNA interference with IML2 induced a significant reduction in the transcript levels of various antimicrobial peptides. Importantly, we found that the abundance of O. sinensis blastospores coated with rIML2 dramatically decreased in the host hemolymph. Overall, this study demonstrated that T. xiaojinensis IML2 modulates cellular and humoral responses to entomopathogenic fungi, broadening our view of the immune interaction between O. sinensis and its host.

16.
Angew Chem Int Ed Engl ; : e202204661, 2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35445780

RESUMO

Oxidizing CH4 into liquid products with O2 under mild conditions still mainly relies on metal catalysis. We prepared a series of sulfone-modified conjugated organic polymers and found that the catalyst with proper SVI content (0.10) could drive O2 →H2 O2 →⋅OH to oxidize CH4 into CH3 OH and HCOOH directly and efficiently at room temperature under light irradiation. Experimental results showed that after 4 h reaction, decomposition rate and residual amounts of H2 O2 were 81.21 % and 4.83 mmol gcat -1 respectively, and CH4 conversion rate was 22.81 %. Mechanism studies revealed that illumination could induce the homolytic dissociation of S=O bonds on catalyst to produce oxygen and sulfur radicals, where the ⋅O could adsorb and activate CH4 , and the ⋅S could supply electrons for 1 O2 to generate H2 O2 and then for decomposing the H2 O2 into ⋅OH timely to oxidize CH4 . This research provided a novel organic catalysis approach for oxygen activation and utilization.

17.
Carbohydr Polym ; 284: 119199, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35287914

RESUMO

The application of flexible multifunctional sensors based on conductive hydrogels in human health detection has been widely studied. Herein, a facile one-pot method is proposed to prepare ionic conductive hydrogels by dissolving polyvinyl alcohol (PVA), cellulose nanofiber (CNF), and aluminum chloride hexahydrate (AlCl3·6H2O) in a dimethyl sulfoxide (DMSO)/water binary solvent. The resulting ionically-conductive organohydrogels have high stretchability (up to 696%), fast response (130 ms), wide operating temperature (-50 °C to 50 °C), and long-term stability (30 days). The hydrogel sensor exhibits excellent signal sensing capability (human motion and sound detection signals) and cycling stability (1000 cycles) under extreme temperature and long-term storage conditions. Notably, the organohydrogel displays high sensitivity to both compressive deformation and temperature, resulting in multifunctional sensing performance. This work provides a viable approach for the long-term use of hydrogels as wearable devices in extreme environments and daily life.


Assuntos
Hidrogéis , Nanofibras , Celulose , Condutividade Elétrica , Humanos , Álcool de Polivinil
18.
Artigo em Inglês | MEDLINE | ID: mdl-35304628

RESUMO

PURPOSE: Toothache, a common disorder afflicting most people, shows distinct features at different clinical stages. This study aimed to depict metabolic changes in brain and investigate the potential mechanism involved in the aberrant affective behaviors during the natural process of toothache. METHODS: We investigated the spatiotemporal patterns of brain function during the natural course of toothache in a rat model of dental pulp injury (DPI) by using positron emission tomography (PET). RESULTS: Glucose metabolism peaked on the 3rd day and gradually decreased in several brain regions after DPI, which was in line with the behavioral and histological results. PET imaging showed that visual pathway was involved in the regulation of toothache. Meanwhile, the process of emotional regulation underlying toothache was mediated by N-methyl-D-aspartic receptor subunit 2B (NR2B) in the caudal anterior cingulate cortex (cACC). CONCLUSION: Our results revealed the spatiotemporal neurofunctional patterns during toothache process and preliminarily elucidated the role of NR2B in cACC in the regulation of toothache-related affective behaviors.

20.
J Oncol ; 2022: 3024360, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35342399

RESUMO

Introduction: The t(8 ; 21) translocation is the most common chromosomal abnormality in human acute myeloid leukemia (AML) subtype 2 (M2), which forms the AML/ETO fusion gene. However, AML/ETO alone does not necessarily cause leukemia. Other factors are thought to contribute to the disease. Calcitonin receptor-like (CALCRL), a G-protein-coupled neuropeptide receptor, is involved in various biological processes, such as colony formation and drug resistance. Methods: First, The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were used to determine any differences in CALCRL expression in AML patients with and without AML/ETO and the prognostic significance of CALCRL expression in AML patients was further evaluated. Next, we detected the CALCRL expression level in 67 AML/ETO+ AML patients and 16 patients with nonmalignant hematological diseases using qRT-PCR and identified its prognostic relevance. Results: Individuals in the group expressing low levels of CALCRL had a longer median survival time. In AML/ETO+ AML patients, higher mRNA levels of CALCRL were observed before treatment, which decreased after the complete remission that followed multiple chemotherapy sessions. Clinical features indicated that more patients in the CALCRLhigh group also had c-kit mutations compared with patients in other groups. Overall survival (OS) was longer in patients with lower levels of CALCRL expression, especially in patients with c-kit mutations or with more blast cells in bone marrow (BM). In addition, a longer OS was observed in the CALCRLlow group after hematopoietic stem cell transplantation (HSCT). Conclusions: This preliminary study indicates that CALCRL could serve as a suitable prognostic factor in AML/ETO+ AML patients.

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