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1.
Chemosphere ; 239: 124737, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31493755

RESUMO

Antibiotics are of concern due to their prevalent detection in aquatic environment. Sulfate radical based advanced oxidation processes show a great capacity to degrade antibiotics, but the mechanisms are still unclear. In this work, the degradation mechanism of fluoroquinolones (FQs), a major group of antibiotics, in UV/Fe2+/PMS was deeply investigated. The degradation process was in-situ and real-time monitoring by illumination-assisted droplet spray ionization mass spectrometry. A series of reactive intermediates were captured, and further characterized by high-resolution mass spectrometry (HRMS) and tandem MS. About 50 different transformation products have been identified for ciprofloxacin and norfloxacin. More than 15 products were the first time reported. Taking into consideration of the sequential formation and intensity change of intermediates, the feasible and complete transformation pathways of FQs were proposed. Compared with the photolysis process, the defluorination of FQs was not observed in this system. This work provided abundant information of FQs degradation by persulfate advanced oxidation processes (AOPs) and meanwhile demonstrated the importance of HRMS and on-line MS in mechanism research of AOPs.

2.
Glia ; 68(1): 111-127, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31444939

RESUMO

Upon retina injury, Müller glia in the zebrafish retina respond by generating multipotent progenitors to repair the retina. However, the complete mechanisms underlying retina regeneration remain elusive. Here we report inflammation-induced mammalian target of rapamycin (mTOR) signaling in the Müller glia is essential for retina regeneration in adult zebrafish. We show after a stab injury, mTOR is rapidly activated in Müller glia and later Müller glia-derived progenitor cells (MGPCs). Importantly, mTOR is required for Müller glia dedifferentiation, as well as the proliferation of Müller glia and MGPCs. Interestingly, transient mTOR inhibition by rapamycin only reversibly suppresses MGPC proliferation, while its longer suppression by knocking down Raptor significantly inhibits the regeneration of retinal neurons. We further show mTOR promotes retina regeneration by regulating the mRNA expression of key reprogramming factors ascl1a and lin-28a, cell cycle-related genes and critical cytokines. Surprisingly, we identify microglia/macrophage-mediated inflammation as an important upstream regulator of mTOR in the Müller glia and it promotes retina regeneration through mTOR. Our study not only demonstrates the important functions of mTOR but also reveals an interesting link between inflammation and the mTOR signaling during retina regeneration.

3.
J Cell Physiol ; 235(2): 1674-1688, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31432509

RESUMO

Renal interstitial fibrosis is a key factor in the development of chronic renal diseases, possibly leading to uremia. The present study conducted aimed to assess the hypothesis whether keratin 1 (KRT1) silencing could suppress kidney interstitial fibrosis and glomerular sclerosis via the Notch pathway to alleviate uremic symptoms. Differentially expressed genes associated with uremia were identified using the gene expression omnibus (GEO) database. Uremic rat models were established, in which short hairpin-RNA against KRT1, activators, and inhibitors of the Notch pathway were transfected. To further validate the mechanism of KRT1 in uremia, KRT1 expression, cell apoptosis, glomerular area (GA), and glomerular capillary volume (GV), the score of glomerular sclerosis, and tubulointerstitial injury were assayed and investigated. GEO database revealed that KRT1 was upregulated in uremia and regulated the Notch pathway. GA, GV, cell apoptosis, glomerular sclerosis, and tubulointerstitial injury were typically located in more elevated levels of uremia in rats. KRT1 silencing and Notch pathway inhibition decreased the expression of Jagged1, Notch1, NICD1, Hey1, Hes1, α-SMA, and FN, which further resulted in decreased cell apoptosis, GA, GV, the score of glomerular sclerosis, and tubulointerstitial injury. Subsequently, the effect of KRT1 silencing on uremia was no longer evident once the Notch pathway was activated. The co-localization of high expression KRT1 and Notch1 was found in uremia. In summary, the results identified KRT1 as a key regulator in uremia progression, and KRT1 silencing can suppress glomerular sclerosis and tubulointerstitial injury via inactivation of the Notch pathway in uremic rats.

4.
Food Chem ; 308: 125596, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31648097

RESUMO

The protective mechanism of glycerol on ß-lactoglobulin were studied in 0-60% glycerol solutions by experimental and molecular simulation approaches. Results showed that the stability of ß-lactoglobulin increased with glycerol concentration, with little secondary structure changes induced by glycerol. The tertiary structure altered slightly with glycerol concentration, resulting in a stronger near UV circular dichroism signal and intrinsic tryptophan fluorescence quenching, indicating aromatic side chains closer to hydrophobic microenvironment. The Rg of ß-lactoglobulin increased with glycerol concentration without dimer dissociation, due to expansion of the quaternary structures. Moreover, the flexibility (RMSF) of ß-lactoglobulin decreased by glycerol. Distance between areas enclosing Asp33 and Arg40 from separate chains did not increase, suggesting possibly reinforced electrostatic attractions. In conclusion, the stabilization of ß-lactoglobulin in glycerol solution is probably the comprehensive results of the decreased molecular flexibility, the strengthened hydrophobic interaction in individual chain, and the possibly reinforced electrostatic attractions between two chains of ß-lactoglobulin.

5.
Food Chem ; 306: 125627, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31610328

RESUMO

Effects of various concentrations of Kadozan (chitosan) treatment on storability and quality properties of harvested 'Fuyan' longans were investigated. Compared to the control samples, Kadozan treated-longans displayed lower fruit respiration rate, lower pericarp cell membrane permeability, pericarp browning index, pulp breakdown index, fruit disease index, and weight loss, but higher rate of commercially acceptable fruit, higher levels of pericarp chlorophyll, carotenoid, anthocyanin, flavonoid and total phenolics, higher amounts of pulp total soluble sugar, sucrose, total soluble solids, and vitamin C. These results revealed Kadozan treatment could increase storability and retain better quality of harvested longan fruit. Among different concentrations of Kadozan, the dilution of 1:500 (VKadozan: VKadozan + Water) showed the best results in storability and maintained the best quality of longans during storage. These findings demonstrated that Kadozan could be a facile and eco-friendly postharvest handling approach for increasing storability and lengthening shelf-life of harvested 'Fuyan' longan fruit.

6.
J Nanosci Nanotechnol ; 20(3): 1866-1872, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492354

RESUMO

Upconversion nanocrystals with uniform size and hydrophilic surface have potential applications in biological medical engineering. In this study, hydrophilic NaYF4:Yb, Er nanospheres were synthesized via hexadecylpyridinium chloride (CPC) mediated hydrothermal process. The synthesized NaYF4:Yb, Er upconversion nanospheres (UCNSs) were characterized by various characterization methods. Results showed that the synthesized UCNSs exhibited a uniform sphere-like structure with average diameter of ~250 nm. The surface of UCNSs was captured by CPC molecule indicating hydrophilic properties of UCNSs. The spherical UCNSs composed of mixed phase (α+ß) NaYF4 nanocrystals. This is the novel study for synthesis of NaYF4:Ln3+ crystal employing CPC as ligand. Moreover, the effect of CPC concentration on synthesis of UCNSs was investigated by comparatively studying morphology, crystal phase and luminescent properties of desired sample prepared under different experimental conditions. It was found that high concentrations of CPC ligands were more favorable for forming NaYF4 crystal nanospheres with morphology that is more regular, have smoother surface, with higher crystallinity and better upconversion fluorescent properties. The possible growing mechanism was proposed and growing of NaYF4:Yb, Er nanospheres followed the classical Ostwald ripening process.

7.
J Cell Physiol ; 235(2): 1209-1221, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31276209

RESUMO

OBJECTIVE: Parathyroid hormone (PTH) is a main systemic mediator of calcium and phosphate homeostasis in the bone. Dental pulp stem cells (DPSCs) have been extensively studied in the regeneration of bone and tooth tissues. This paper aims to uncover the influences of PTH on the proliferative ability and osteo/odontogenic differentiation of DPSCs, as well as the underlying mechanisms. MATERIALS AND METHODS: The optimal concentration of PTH on DPSCs was determined by alkaline phosphatase (ALP) activity assay, ALP staining and western blot analysis. Proliferative ability and cell cycle distribution of DPSCs were analyzed by Cell counting kit-8, 5-ethynyl-20-deoxyuridine assay, and flow cytometry. Osteo/odontogenic capacity of DPSCs was evaluated and finally, the involvement of mitogen-activated protein kinase (MAPK) pathway was assessed. RESULTS: Purified DPSCs were obtained by enzymatic digestion, which presented a typical fibroblast-like morphology. 10-9 mol/L PTH was concerned as the optimal concentration for DPSCs induction. 10-9 mol/L PTH treatment did not change the proliferative rate of DPSCs (p > .05). Relative expressions of DSPP/DSPP, RUNX2/RUNX2, OSX/OSX, and ALP/ALP were upregulated in PTH-treated DPSCs relative to control group. Particularly, their mRNA/protein levels at Day 7 were markedly higher relative to those at Day 3 (p < .05 or p < .01). Mineralized nodules were formed after PTH induction, and calcium content increased by cetylpyridinium chloride quantitative analysis. Mechanistically, the protein levels of p-ERK and p-P38 significantly increased after PTH treatment, and the inhibitors targeting MAPK were identified that weakened the effects of PTH on the committed differentiation of DPSCs. CONCLUSIONS: PTH enhances the osteo/odontogenic differentiation capacity of DPSCs via ERK and P38 signaling pathways.

8.
Biosens Bioelectron ; 147: 111755, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31630032

RESUMO

In cell signal transduction pathways, a series of biochemical reactions and interactions between proteins guarantee physiological responses indicating cell functionality. However, there are a variety of upstream and downstream signal molecules in these pathways with multiple levels of cross-regulation, making it difficult to sequential visualize their relationships in living organisms in complex environments. To investigate the interrelationships among intracellular signaling pathways, a Au-Se bonded nanoprobe with extraordinary stability and strong anti-interference ability was designed and prepared to monitor the evolution of two kinds of apoptosis biomarkers in real time. Two different peptide chains decorated with two dyes were functionalized on the surface of Au nanoparticles (NPs) via Au-Se bonds. These peptide chains can be respectively cleaved by upstream cathepsin B proteins and downstream caspase-3 proteins to trigger fluorescence recovery. Moreover, when the living cells were stimulated to induce the apoptotic pathway, cathepsin B and caspase-3 were activated in turn with signals sequentially recovered at 2 and 4 h, respectively. This fluorescent nanoprobe can be used in complicated biological systems to achieve real-time in situ monitoring of the sequential activation of signal molecules in intracellular pathways and provides a novel approach for the future investigation of protein interactions in vivo.

9.
Chemosphere ; 241: 125106, 2019 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-31683428

RESUMO

A pot experiment was conducted to investigate the possible mediatory effect of organic amendments (vermicompost and biochar) and selenium (Se) on Cd bioaccumulation in both rice cultivars (high-Cd accumulation rice: Yuzhenxiang (YZX) and low-Cd accumulation rice: Changliangyou772 (CLY)) in high-Cd-contaminated soils. The results showed that Cd sensitivity and tolerance were cultivar-dependent, and grain Cd contents for CLY accorded with the Chinese national food safety standards (0.2 mg kg-1), whereas grain Cd levels for YZX were 1.4-5.8 times higher than those for CLY. Soil applications of amendments decreased grain Cd levels by 3.5%-36.9% for YZX and 36.1%-74.4% for CLY. Moreover, vermicompost (VC) was more effective in reducing Cd bioaccumulation than biochar (BC). A combination of Se and organic amendments could significantly increase grain Se contents and help further reduce grain Cd levels by 5.8%-20.8%, compared to the single organic amendments. This mitigation progress could be attributed to the changes of Cd translocation and distribution among rice tissues and the inhibition of Cd bioavailability in soil through the alteration in soil properties. Organic amendments, especially high dose (5%), increased soil pH and organic matter contents, and correspondingly decreased soil Cd bioavailability. A sequential extraction analysis suggested that organic amendments and Se facilitated the transformation of soil Cd from the bioavailable form to the immobilized Cd form, and thus decreased grain Cd levels. Hence, co-applications of organic amendments and Se in combination with low-Cd accumulation cultivar could be an effective strategy for both Se needs of humans and safe utilization of Cd polluted soil.

10.
Nutrients ; 11(11)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31683900

RESUMO

BACKGROUND: Limited data were available on trends of US dietary nutrients especially for specific subgroups; Methods: Dietary intakes of energy and 36 kinds of nutrients were analyzed in the National Health and Nutrition Examination Survey (NHANES) from 2003 to 2016 and by age and sex, socioeconomic status, race/ethnicity, and body mass index, which were evaluated by whether not they meet the dietary reference intakes (DRIs); Results: Significantly decreased trends were observed for carbohydrate, total sugars, fiber, calcium, magnesium, phosphorus, selenium, vitamin B6, E, K, and choline, while increased trends were observed for saturated fatty acids, iron, zinc, copper, potassium, sodium, vitamin B1, B2, B12, C and folate DFE (as dietary folate equivalents). A decreased trend of exceeding the estimated energy requirement was found. Population with low socioeconomic status and non-Hispanic blacks accounted for the largest proportion not meeting DRIs for most of nutrients; Conclusions: Most dietary nutrients were improved among US adults from 2003 to 2016 but were still far from optimal levels. Populations with low socioeconomic status and non-Hispanic blacks should be paid more attention to improve their dietary nutrient intake.

11.
Opt Express ; 27(20): 28760-28781, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684621

RESUMO

The reflectance factor acquired in laboratory makes a good contribution to spectral library for remote sensing. However, this reflectance factor is a different quantity from that acquired in the field. To bridge the gap between them, the spectral and the spatial characteristics of sunlight and skylight are analyzed. A facility called MHSRS2F is introduced to acquire the anisotropy reflectance in laboratory under the illumination of simulated sunlight and skylight. This reflectance factor is the same quantity as that acquired in field. The amplitude and shape differences between the reflectance factors acquired in laboratory and in field are reduced for 27% and 31% reflectively.

12.
Rev Esp Enferm Dig ; 1112019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31718212

RESUMO

BACKGROUND: interleukin-37 (IL-37) is as a natural suppressor of the innate inflammatory and immune responses. It has also been reported to be involved in carcinogenesis and metastasis. The present case-control study was designed to investigate the role of serum levels of IL-37 in patients with gastric cancer. METHODS: serum IL-37 levels were determined using the enzyme-linked immunosorbent assay in 180 patients diagnosed with gastric cancer and 100 healthy controls. The association between IL-37 levels and clinical factors was assessed. Univariate and multivariate analyses were performed to investigate the prognostic significance of these parameters in gastric cancer. RESULTS: serum IL-37 levels in gastric cancer patients (5.606 ± 0.837 pg/ml) were significantly higher than those in healthy controls (2.364 ± 0.210 pg/ml, p < 0.001). High serum IL-37 levels were related to a poorly differentiated histologic type (p = 0.046) and advanced T stage (p = 0.003). The Kaplan-Meier survival analysis indicated that the high-IL-37 group had a poorer overall survival and progression-free survival (overall survival [OS]: 39.0 months vs 13.0 months, p < 0.001, progression-free survival [PFS]: 25.0 months vs 10.0 months, p < 0.001). Multivariate analyses showed serum IL-37 to be an independent prognostic factor for gastric cancer patients (OS: hazard ratios [HR] = 1.842, 95% CI: 1.190-2.854, p = 0.006; PFS: HR = 1.547, 95% CI: 1.014-2.359, p = 0.043). CONCLUSIONS: in conclusion, serum IL-37 levels were associated with poor overall survival and progression-free survival in gastric cancer patients. IL-37 may be a potential predictor of prognosis in gastric cancer.

13.
Environ Pollut ; : 113403, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31711721

RESUMO

Both air pollution and dyslipidemias contributed to large number of deaths and disability-adjusted life lost years. Long-term air pollution exposure was related to changed blood lipids and risk of dyslipidemias. This study was designed to evaluate relationships between air pollutants, blood lipids and prevalence of dyslipidemias in a Chinese rural population exposed to high-level air pollution based on baseline data of The Henan Rural Cohort study. An amount of 39,057 participants from rural areas in China were included. The 3-year average exposure of air pollutants (PM2.5, PM10, NO2) was estimated by a spatiotemporal model. Logistic and linear regression models were employed to explore relationships between air pollutants, blood lipids (TC, TG, HDL-C and LDL-C) and prevalence of dyslipidemias. The three-year concentration of PM2.5, PM10 and NO2 was 72.8 ±â€¯2.3 µg/m3, 131.5 ±â€¯5.7 µg/m3and 39.1 ±â€¯3.1 µg/m3, respectively. Overall, increased air pollution exposure was related to increased TC and LDL-C, while decreased TG and HDL-C. Each 1-µg/m3 increment of PM2.5 was related to 0.10% (0.07%-0.19%) increase in TC, 0.63% (0.50%-0.77%) increase in LDL-C, 2.93% (2.70%-3.16%) decrease in TG, 0.49% (0.38%-0.60%) decrease in HDL-C; and 5.7% (95%CI: 3.7%-7.6%), 4.0% (95%CI: 2.1%-6.0%) and 3.8% (95%CI: 2.5%-5.1%) increase in odds for hypercholesterolemia, hyperbetalipoproteinemia and hypoalphalipoproteinemia, respectively. Stronger associations were found in male and older participants. Findings suggest that air pollutants were associated with changed blood lipid levels and higher risk of dyslipidemias among rural population. Male and elder people should pay more attention to personal safety protection.

14.
Nat Genet ; 51(11): 1607-1615, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31676864

RESUMO

Melon is an economically important fruit crop that has been cultivated for thousands of years; however, the genetic basis and history of its domestication still remain largely unknown. Here we report a comprehensive map of the genomic variation in melon derived from the resequencing of 1,175 accessions, which represent the global diversity of the species. Our results suggest that three independent domestication events occurred in melon, two in India and one in Africa. We detected two independent sets of domestication sweeps, resulting in diverse characteristics of the two subspecies melo and agrestis during melon breeding. Genome-wide association studies for 16 agronomic traits identified 208 loci significantly associated with fruit mass, quality and morphological characters. This study sheds light on the domestication history of melon and provides a valuable resource for genomics-assisted breeding of this important crop.

15.
Medicine (Baltimore) ; 98(44): e17708, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689803

RESUMO

The aim of this study was to assess the effect of preoperative sleep quality on acute postoperative pain in breast cancer patients.The Pittsburgh Sleep Quality Index questionnaire (PSQI) was used to assess the overall sleep status of women scheduled for unilateral modified radical mastectomy in the past month. Based on the responses, patients were allocated to good sleep group or poor sleep group. Postoperatively, acute pain was assessed using the numerical rating score in the first 24 hours; in addition, the requirement of analgesics and the incidence of postoperative complications were recorded.A total of 108 breast surgery patients were enrolled. Based on the PSQI results, 55 (51%) patients were allocated to poor sleep group and 53 (49%) to good sleep group. Pain scores were similar in the 2 groups at the end of surgery (P = .589); however, poor sleep group reported higher postoperative pain scores than the good sleep group at 2 (P = .002), 6 (P < .001), 12 (P < .001), and 24 (P = .002) hours after surgery. The incidence of severe pain in the poor sleep group was higher than that in the good sleep group (27% vs 8%, P = .018), and the ratio of participants who required rescued analgesics was greater in the poor sleep group (52% vs 22%, P = .002). In addition, patients with poor sleep quality had more postoperative complications and longer hospital stay.In this study, breast cancer patients with poor preoperative sleep quality reported more severe postoperative pain, required more analgesics, experienced more complications, and had longer hospital stay.


Assuntos
Neoplasias da Mama/cirurgia , Mastectomia/efeitos adversos , Dor Pós-Operatória/etiologia , Distúrbios do Início e da Manutenção do Sono/complicações , Sono , Adolescente , Adulto , Idoso , Analgésicos/uso terapêutico , Neoplasias da Mama/complicações , Neoplasias da Mama/fisiopatologia , Feminino , Humanos , Tempo de Internação , Pessoa de Meia-Idade , Medição da Dor , Dor Pós-Operatória/tratamento farmacológico , Período Pré-Operatório , Estudos Prospectivos , Fatores de Risco , Distúrbios do Início e da Manutenção do Sono/fisiopatologia , Adulto Jovem
16.
Cell Biochem Funct ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31693217

RESUMO

The fat-derived protein adiponectin is known to reverse the effects of insulin resistance and to lower blood glucose levels. The AMP-activated protein kinase (AMPK) signalling pathway plays a central role in metabolism and energy homeostasis. Here, to investigate the role of AMPK in the protective effect of adiponectin against insulin resistance, we established the model of high-glucose (HG)- and high-lipid (HL)-induced insulin resistance in INS-1 pancreatic ß cells. We found that 25mM of glucose and 0.4mM of palmitic acid treatment significantly increased cell apoptosis and impaired insulin secretion in INS-1 cells. However, recombinant human adiponectin dramatically reduced HG- and/or HL-induced cell apoptosis and greatly improved insulin secretion. Interestingly, adiponectin treatment also activated AMPK signalling pathway by increasing the phosphorylation of Thr172 in the AMPK α subunit; 10µM of compound C, a potent AMPK inhibitor, blocked the protective effects of adiponectin against HG/HL-induced insulin resistance. Furthermore, knockout experiments by CRISPR/Cas9 technology showed that AMPK α1, but not AMPK α2, is involved in the protective effects of adiponectin. Taken together, adiponectin reversed the effects of insulin resistance via AMPK α1, which provides a novel insight into the protective mechanism of adiponectin and may be used as a new strategy for the treatment of type 2 diabetes. SIGNIFICANCE OF THE STUDY: Adiponectin can reverse the effects of insulin resistance and lower blood glucose levels. Here, adiponectin reduced HG/HL-induced cell apoptosis and greatly improved insulin secretion. These effects were blocked by AMPK inhibitor, compound C. Specifically, we found that AMPK α1, but not AMPK α2, mediates the protective effects of adiponectin, which provides a novel insight into the protective mechanism of adiponectin against insulin resistance.

17.
Ocul Immunol Inflamm ; : 1-9, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31671006

RESUMO

Purpose: To investigate the association of CARD9 gene polymorphisms with Behcet's disease (BD) and acute anterior uveitis (AAU) in a Chinese Han population.Methods: We performed a case-control association study in 480 patients with BD, 1151 patients with AAU and 1440 healthy controls. Six single nucleotide polymorphisms (SNPs) of CARD9 were genotyped, including rs4077515, rs11145769, rs59902911, rs9411205, rs4073153 and rs1135314.Results: None of the individual SNPs in the CARD9 gene showed an association with either BD or AAU. Haplotype analysis revealed a significant decrease of the frequency of a CARD9 gene haplotype CGCCA (rs4077515, rs11145769, rs59902911, rs9411205, rs4073153) in BD when compared to healthy controls (Pc = 0.012, OR = 0.585, 95%CI = 0.409 ~ 0.837). Haplotype analysis did not show an association between CARD9 and AAU.Conclusions: This study shows that a five-SNP haplotype of the CARD9 gene (CGCCA) may be a protective factor for BD with ocular involvement, but not for AAU.

18.
Sci Rep ; 9(1): 17264, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31754170

RESUMO

This study contrasted the impact of Tai Chi Chuan and general aerobic exercise on brain plasticity in terms of an increased grey matter volume and functional connectivity during structural magnetic resonance imaging (sMRI) and resting-state functional magnetic resonance imaging (rs-fMRI), explored the advantages of Tai Chi Chuan in improving brain structure and function. Thirty-six college students were grouped into Tai Chi Chuan (Bafa Wubu of Tai Chi), general aerobic exercise (brisk walking) and control groups. Individuals were assessed with a sMRI and rs-fMRI scan before and after an 8-week training period. The VBM toolbox was used to conduct grey matter volume analyses. The CONN toolbox was used to conduct several seed-to-voxel functional connectivity analyses. We can conclude that compared with general aerobic exercise, eight weeks of Tai Chi Chuan exercise has a stronger effect on brain plasticity, which is embodied in the increase of grey matter volume in left middle occipital gyrus, left superior temporal gyrus and right middle temporal gyrus and the enhancement of functional connectivity between the left middle frontal gyrus and left superior parietal lobule. These findings demonstrate the potential and advantages of Tai Chi Chuan exercises in eliciting brain plasticity.

19.
Genome Biol ; 20(1): 227, 2019 11 08.
Artigo em Inglês | MEDLINE | ID: mdl-31699133

RESUMO

We present the software Condition-specific Regulatory Units Prediction (CRUP) to infer from epigenetic marks a list of regulatory units consisting of dynamically changing enhancers with their target genes. The workflow consists of a novel pre-trained enhancer predictor that can be reliably applied across cell types and species, solely based on histone modification ChIP-seq data. Enhancers are subsequently assigned to different conditions and correlated with gene expression to derive regulatory units. We thoroughly test and then apply CRUP to a rheumatoid arthritis model, identifying enhancer-gene pairs comprising known disease genes as well as new candidate genes.

20.
Aging (Albany NY) ; 11(21): 9661-9671, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31699957

RESUMO

Abnormal dopaminergic modulation of the cortico-basal ganglia motor loops results in the emergence of levodopa-induced dyskinesia (LID). We focused on alterations in the gray matter (GM) volume and the cortical thickness of the brain, especially in cortico-basal ganglia motor loops, in Parkinson's disease (PD) with diphasic dyskinesia. 48 PD patients with diphasic dyskinesia, 60 PD patients without dyskinesia and 48 healthy controls (HC) were included. Voxel-based morphometry (VBM) was applied to get GM images from MRI brain images. FreeSurfer was used to get cortical thickness. Distinct analyses of covariance (ANCOVA) and linear contrasts were performed for early- and late-onset PD groups. The severity of diphasic dyskinesia was evaluated by the Unified Dyskinesia Rating Scale (UDysRS). Finally, the correlations between mean volumes of clusters showing differences and the UDysRS scores were performed by Pearson's correlation. The GM volumes of precentral gyri were increased in PD patients with diphasic dyskinesia when compared with those without dyskinesia, which were positively correlated with UDysRS scores in PD patients with diphasic dyskinesia. However, there was no significant difference in cortical thickness among groups. The increased precentral gyri GM volumes might be associated with the pathogenesis and the severity of diphasic dyskinesia.

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