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1.
Med Sci Monit ; 27: e928374, 2021 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-33388740

RESUMO

BACKGROUND Although influenza primarily affects the respiratory system, it can cause severe neurological complications, especially in younger children, but knowledge about the early indicators of acute necrotizing encephalopathy (ANE) is limited. The main purpose of this article is to summarize the clinical characteristics, diagnosis, and treatment of neurological complications of influenza in children, and to identify factors associated with ANE. MATERIAL AND METHODS This was a retrospective study of children with confirmed influenza with neurological complications treated between 01/2014 and 12/2019 at Guangzhou Women and Children's Medical Center. A receiver operating characteristics curve analysis was performed to determine the prognostic value of selected variables. RESULTS Sixty-three children with IAE (n=33) and ANE (n=30) were included. Compared with the IAE group, the ANE group showed higher proportions of fever and acute disturbance of consciousness, higher alanine aminotransferase, higher aspartate aminotransferase, higher creatinine kinase, higher procalcitonin, higher cerebrospinal fluid (CSF) protein, and lower CSF white blood cells (all P<0.05). The areas under the curve (AUCs) for procalcitonin and CSF proteins, used to differentiate IAE and ANE, were 0.790 and 0.736, respectively. The sensitivity and specificity of PCT >4.25 ng/ml to predict ANE were 73.3% and 100.0%, respectively. The sensitivity and specificity of CSF protein >0.48 g/L to predict ANE were 76.7% and 69.7%, respectively. Thirteen (43.3%) children with ANE and none with IAE died (P<0.0001). CONCLUSIONS High levels of CSF protein and serum procalcitonin might be used as early indicators for ANE. All children admitted with neurological findings, especially during the influenza season, should be evaluated for influenza-related neurological complications.

2.
Med Sci Monit ; 26: e928835, 2020 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-33335084

RESUMO

BACKGROUND This study summarizes the characteristics of children screened for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and reports the case of 1 child who was diagnosed with SARS-CoV-2 infection in Guangzhou Women and Children's Medical Center and the cases of his family members. MATERIAL AND METHODS The medical records of 159 children who were admitted to our hospital from January 23 to March 20, 2020, were retrospectively analyzed. Samples from pharyngeal or/and anal swabs were subjected to reverse-transcription polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 within 12 h of patient admission; a second RT-PCR test was done 24 h after the first test. RESULTS Of the 159 patients, 151 patients had epidemiological histories, 14 patients had cluster onset, and 8 patients had no epidemiological history but had symptoms similar to coronavirus disease 2019 (COVID-19). The most common symptom was fever (n=125), followed by respiratory and gastrointestinal symptoms. A 7-year-old boy in a cluster family from Wuhan was confirmed with asymptomatic SARS-CoV-2 infection with ground-glass opacity shadows on his lung computed tomography scan, and his swab RT-PCR test had not turned negative until day 19 of his hospitalization. In patients who did not test positive for SARS-CoV-2, influenza, respiratory syncytial virus, and adenovirus were observed. A total of 158 patients recovered, were discharged, and experienced no abnormalities during follow-up. CONCLUSIONS For SARS-CoV-2 nosocomial infections, taking a "standard prevention & contact isolation & droplet isolation & air isolation" strategy can prevent infection effectively. Children with clustered disease need close monitoring.


Assuntos
/diagnóstico , /epidemiologia , /métodos , Criança , Pré-Escolar , China/epidemiologia , Coronavirus/metabolismo , Coronavirus/patogenicidade , Infecção Hospitalar/epidemiologia , Feminino , Febre , Hospitalização , Hospitais , Humanos , Masculino , Registros Médicos , Alta do Paciente , Estudos Retrospectivos , /patogenicidade
3.
Cell Death Dis ; 11(12): 1065, 2020 12 14.
Artigo em Inglês | MEDLINE | ID: mdl-33311442

RESUMO

Circular RNAs (circRNAs) have confirmed to participate in diverse biological functions in cancer. However, the expression patterns of circRNAs on hepatocellular carcinoma (HCC) remains unclear. In the present study, we clarified that hsa_circRNA_104348 was dramatically upregulated in HCC tissues and cells. Patients with HCC displaying high hsa_circRNA_104348 level possessed poor prognosis. Has_circ_104348 facilitated proliferation, migration, and invasion, meanwhile suppressed apoptosis of HCC cell. Furthermore, hsa_circRNA_104348 directly targeted miR-187-3p, could regulate miR-187-3p to affect proliferation, migration, invasion, and apoptosis of HCC cells, and may have effect on Wnt/ß-catenin signaling pathway. Moreover, RTKN2 could be a direct target of miR-187-3p. In addition, knockdown of hsa_circRNA_104348 attenuated HCC tumorigenesis and lung metastasis in vivo. Taken together, these findings indicated that circular RNA hsa_circRNA_104348 might function as a competing endogenous RNA (ceRNA) to promotes HCC progression by targeting miR-187-3p/RTKN2 axis and activating Wnt/ß-catenin pathway.

4.
J Spec Pediatr Nurs ; 25(4): e12305, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32702207

RESUMO

PURPOSE: Because the quality of medical resources is extremely uneven across China, it is nearly impossible to implement a unified emergency triage program. The aim of the study is to examine triage using the "two-step four-level+ " triage model in a hospital in Southern China, with an emphasis on hand, foot, and mouth disease. DESIGN AND METHODS: This was a retrospective study of all patients seen in the pediatric emergency room (ER) between January 1, 2012 and December 31, 2018, at the Guangzhou Women and Children's Medical Center. The "two-step and four-level+ " was manually implemented in 2012, and an electronic triage system was developed and applied since 2015. Emergency quality control indicators were analyzed. RESULTS: There were 645,473 patients triaged at the pediatric ER between January 1, 2015 and December 31, 2018. After the first step, 17,444 patients were classified as unstable, including 6546 (1.01%) Level I patients, 10,898 (1.69%) Level II patients, 210,368 (32.5%) Level III patients, and 417,661 (64.8%) Level IV patients. After triage implementation, the stay time of the patient in the pediatric ER decreased each year (all p < .05) and shortened to 20.3 ± 2.2 h in 2018. Compared with 2012-2014, the mortality of 2015-2018 decreased by 21.1%, the rate of unexpected resuscitation was 0%, and the complaints of overcrowding decreased (all p < .05). PRACTICE IMPLICATIONS: This "two-step four-level+ " triage method can improve the medical care quality of pediatric ER in China.

5.
Clin Lab ; 66(5)2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32390398

RESUMO

BACKGROUND: Pompe disease is an inherited neuromuscular disease caused by a deficiency of lysosomal acid alphaglucosidase (GAA) leading to glycogen accumulation in muscle and motor neurons. METHODS: We retrospectively collected the clinical information on the case of a 2-year-old child admitted to the emergency department with respiratory failure and generalized hypotonia. RESULTS: Chest radiograph, cerebral and spinal magnetic resonance imaging, muscle biopsy, etc. were all examined. The 2-year-old child was later diagnosed with Pompe disease. Systematic assessment and effective sedation management were applied. CONCLUSIONS: Differential diagnosis, systematic assessment, and effective sedation management are important for Pompe disease patients.

6.
World J Clin Cases ; 8(2): 382-389, 2020 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-32047789

RESUMO

BACKGROUND: Influenza in children is a major cause of morbidity and mortality worldwide. Nervous system diseases are a factor relating to increased mortality rate. However, reports of how these underlying diseases contribute to the death of children with influenza are rare. CASE SUMMARY: A 4-year-old-girl developed type A influenza-related encephalopathy (IAE) with seizures, acute disorder of consciousness, and intracranial hypertension (cerebrospinal fluid pressure: 250 mmH2O), and the Dandy-Walker variant was found by her first magnetic resonance imaging (MRI) when admission. Three days later, she suddenly presented anisocoria, acute pulmonary edema, and coma, and the later MRI found that she had compressed brainstem, oblongata "Z-like folding", and swelling bilateral basal ganglia. After admission, the patient were tested for routine and special biomarkers and underwent neuroimaging and neuroelectrophysiology examinations as well as Oseltamivir and intravenous immunogloblin treatments. When predicting that unstable intracranial structures detected by MRI might have disastrous consequences in the progression of IAE, she was transferred into the pediatric intensive care unit and underwent continuous assessment of clinical condition while she did not have instability of basic vital signs; at the same time, her parents were fully informed about the risk and prognosis. Although she was ultimately dead from brain stem failure, the parents expressed understanding and did not trigger a doctor-patient conflict. CONCLUSION: In case of finding an unstable intracranial structure, intensive care should be given to IAE patient and their clinical condition should be monitored continuously.

7.
Int J Mol Med ; 44(2): 759-767, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31173167

RESUMO

Enterovirus 71 (EV71) accounts for the majority of hand, foot and mouth disease­related deaths due to fatal neurological complications. EV71 structural viral protein 1 (VP1) promotes viral replication by inducing autophagy in neuron cells, but the effect of VP1 on myelin cells is unclear. The present study aimed to investigate the role and mechanism of VP1 in autophagy of mouse Schwann cells. An EV71 VP1­expressing vector (pEGFP­C3­VP1) was generated and transfected into mouse Schwann cells. Transmission electron microscopy and western blot analysis for microtubule­associated protein 1 light chain 3 α (LC3) II (an autophagy marker) were used to assess autophagy. Reverse transcription­quantitative PCR and immunofluorescence were performed to determine the expression of peripheral myelin protein 22 (PMP22). Small interfering RNA against PMP22 was used to investigate the role of PMP22 in mouse Schwann cell autophagy. Salubrinal [a selective endoplasmic reticulum (ER) stress inhibitor] was used to determine whether PMP22 expression was affected by ER stress. The present results indicated that VP1 promoted mouse Schwann cell autophagy. Overexpression of VP1 upregulated PMP22. PMP22 deficiency downregulated LC3II and thus inhibited autophagy. Furthermore, PMP22 expression was significantly suppressed by salubrinal. In conclusion, VP1 promoted mouse Schwann cell autophagy through upregulation of ER stress­mediated PMP22 expression. Therefore, the VP1/ER stress/PMP22 autophagy axis may be a potential therapeutic target for EV71 infection­induced fatal neuronal damage.


Assuntos
Enterovirus Humano A/fisiologia , Infecções por Enterovirus/metabolismo , Proteínas da Mielina/metabolismo , Células de Schwann/virologia , Proteínas Estruturais Virais/metabolismo , Animais , Autofagia , Linhagem Celular , Estresse do Retículo Endoplasmático , Infecções por Enterovirus/virologia , Humanos , Camundongos , Células de Schwann/metabolismo , Células de Schwann/patologia
8.
Traffic Inj Prev ; 19(4): 409-416, 2018 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-29265933

RESUMO

OBJECTIVES: To overcome the limitations of previous highway alignment safety evaluation methods, this article presents a highway alignment safety evaluation method based on fault tree analysis (FTA) and the characteristics of vehicle safety boundaries, within the framework of dynamic modeling of the driver-vehicle-road system. METHODS: Approaches for categorizing the vehicle failure modes while driving on highways and the corresponding safety boundaries were comprehensively investigated based on vehicle system dynamics theory. Then, an overall crash probability model was formulated based on FTA considering the risks of 3 failure modes: losing steering capability, losing track-holding capability, and rear-end collision. RESULTS: The proposed method was implemented on a highway segment between Bengbu and Nanjing in China. A driver-vehicle-road multibody dynamics model was developed based on the 3D alignments of the Bengbu to Nanjing section of Ning-Luo expressway using Carsim, and the dynamics indices, such as sideslip angle and, yaw rate were obtained. Then, the average crash probability of each road section was calculated with a fixed-length method. Finally, the average crash probability was validated against the crash frequency per kilometer to demonstrate the accuracy of the proposed method. The results of the regression analysis and correlation analysis indicated good consistency between the results of the safety evaluation and the crash data and that it outperformed the safety evaluation methods used in previous studies. CONCLUSION: The proposed method has the potential to be used in practical engineering applications to identify crash-prone locations and alignment deficiencies on highways in the planning and design phases, as well as those in service.


Assuntos
Acidentes de Trânsito/estatística & dados numéricos , Condução de Veículo/estatística & dados numéricos , Segurança/estatística & dados numéricos , China
9.
BMC Infect Dis ; 17(1): 153, 2017 02 17.
Artigo em Inglês | MEDLINE | ID: mdl-28212620

RESUMO

BACKGROUND: Enterovirus 71 (EV-A71) shows a potential of rapid death, but the natural history of the infection is poorly known. This study aimed to examine the natural history of EV-A71 infection. METHODS: This was a prospective longitudinal observational study performed between January 1st and October 31st, 2012, at three hospitals in Guangdong, China. Subjects with positive EV-A71 RNA laboratory test results were included. Disease progression was documented with MRI, autopsies, and follow-up. Symptoms/signs with potential association with risk of death were analyzed. RESULTS: Among the 288 patients, neurologic symptoms and signs were observed (emotional movement disorders, dyskinesia, involuntary movements, autonomic dysfunction, and disturbance of consciousness). Some of them occurred as initial symptoms. Myoclonic jerks/tremors were observed among >50% of the patients; nearly 40% of patients presented fatigue and 25% were with vomiting. Twenty-eight patients (9.7%) presented poor peripheral perfusion within 53.4 ± 26.1 h; 23 patients (8.0%) presented pulmonary edema and/or hemorrhage within 62.9 ± 28.6 h. Seventeen (5.9%) patients were in a coma. Seven (2.4%) patients died within 62.9 ± 28.6 h. Seventy-seven survivors underwent head and spinal cord MRI and 37.7% (29/77) showed abnormalities. Two fatal cases showed neuronal necrosis, softening, perivascular cuffing, colloid, and neuronophagia phenomenon in the brainstem. CONCLUSIONS: Patients with EV-A71 infection showed high complexity of symptoms and onset timing. Death risk may be indicated by autokinetic eyeball, eyeball ataxia, severe coma, respiratory rhythm abnormality, absent pharyngeal reflex, ultrahyperpyrexia, excessive tachycardia, pulmonary edema and/or hemorrhage, and refractory shock and ataxic respiration. Early assessment of these symptoms/signs is important for proper management.


Assuntos
Encefalite Viral/diagnóstico , Enterovirus Humano A/patogenicidade , Infecções por Enterovirus/diagnóstico , Infecções por Enterovirus/virologia , Hemorragia/diagnóstico , Edema Pulmonar/diagnóstico , Transtornos Respiratórios/diagnóstico , Autopsia , Criança , Pré-Escolar , China/epidemiologia , Coma , Surtos de Doenças , Progressão da Doença , Encefalite Viral/mortalidade , Encefalite Viral/fisiopatologia , Enterovirus Humano A/isolamento & purificação , Infecções por Enterovirus/mortalidade , Infecções por Enterovirus/fisiopatologia , Feminino , Hemorragia/mortalidade , Hemorragia/fisiopatologia , Humanos , Lactente , Estudos Longitudinais , Imagem por Ressonância Magnética , Masculino , Estudos Prospectivos , Edema Pulmonar/mortalidade , Edema Pulmonar/fisiopatologia , Transtornos Respiratórios/mortalidade , Transtornos Respiratórios/fisiopatologia , Taxa Respiratória/fisiologia
10.
Neurotox Res ; 31(2): 204-217, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-27848175

RESUMO

Severe hand-foot-and-mouth disease (HFMD) caused by Enterovirus 71 (EV71) always accompanies with inflammation and neuronal damage in the central nervous system (CNS). During neuronal injuries, cell surface-exposed calreticulin (Ecto-CRT) is an important mediator for primary phagocytosis of viable neurons by microglia. Our data confirmed that brainstem neurons underwent neuronophagia by glia in EV71-induced death cases of HFMD. EV71 capsid proteins VP1, VP2, VP3, or VP4 did not induce apoptosis of brainstem neurons. Interestingly, we found VP1-activated endoplasmic reticulum (ER) stress and autophagy could promote Ecto-CRT upregulation, but ER stress or autophagy alone was not sufficient to induce CRT exposure. Furthermore, we demonstrated that VP1-induced autophagy activation was mediated by ER stress. Meaningfully, we found dexamethasone treatment could attenuate Ecto-CRT upregulation by alleviating VP1-induced ER stress. Altogether, these findings identify VP1-promoted Ecto-CRT upregulation as a novel mechanism of EV71-induced neuronal cell damage and highlight the potential of the use of glucocorticoids to treat severe HFMD patients with CNS complications.


Assuntos
Calreticulina/metabolismo , Proteínas do Capsídeo/toxicidade , Dexametasona/farmacologia , Estresse do Retículo Endoplasmático/fisiologia , Neurônios/fisiologia , Fagocitose/fisiologia , Proteínas Estruturais Virais/toxicidade , Animais , Autofagia/efeitos dos fármacos , Autofagia/fisiologia , Tronco Encefálico/efeitos dos fármacos , Tronco Encefálico/fisiopatologia , Células Cultivadas , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Feminino , Humanos , Masculino , Fagocitose/efeitos dos fármacos , Ratos , Regulação para Cima
11.
Am J Transl Res ; 8(7): 2937-46, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27508014

RESUMO

OBJECTIVE: The study aimed to simulate the microenvironment of gastric cancer to promote the malignant transformation of bone marrow mesenchymal stem cells (BMSCs) and further evaluate the effect of Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions on BMSCs. METHODS: Transwell co-culture was performed on the human gastric cancer cell strains BGC-823 and BMSCs to simulate the microenvironment of gastric cancer. The drug-containing serum prepared by Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions was used, and its influence on BMSCs with malignant transformation was observed. RESULTS: BMSCs were harvested successfully from the rat bone marrow, and flow cytometer identification indicated that CD44+/CD34- cells accounted for 70.64%. The co-culture of BGC-823 cells can induce malignant transformation of BMSCs. And the drug-containing serum can induce G2 phase arrest, inhibit cell proliferation, simultaneously inhibit TERT and c-myc expression, lower the cellular ability of chemotactic migration, inhibit the tumor-forming ability of BGC-823 in nude rats and promote the tumor apoptosis. CONCLUSION: The effective components of Pinelliae Decoction for Purging Stomach-Fire in gastric cancer treatment are pinelliae and dried ginger, and the main acting mechanism is to inhibit tumor cell proliferation and chemotactic migration and promote apoptosis.

12.
Ying Yong Sheng Tai Xue Bao ; 26(11): 3398-404, 2015 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-26915196

RESUMO

Topsoil of green space including typical forest, shrub and grassland were collected to measure their water soluble organic carbon ( WSOC) before and after incubation of 30 days at 5, 15, 25, 35 and, 45 °C. The results showed the average values of WSOC were higher in urban than in rural green spaces, but the percentage of WSOC to total organic carbon (TOC) showed an opposite trend. No significant changes were found among the three green space types in WSOC and WSOC/TOC. Response of WSOC in green space to incubation temperature was generally highest in urban sites, followed by suburban sites, and lowest in rural sites at the incubation temperature of 5 °C, but showed an opposite trend at the temperature of 45 °C. Response coefficient of WSOC to temperature change was lower in forest and shrub than in grassland, but increased along the urban-rural gradient. Further analysis showed that WSOC positively correlated with TOC, total nitrogen and available phosphorus, and the response coefficient of WSOC to temperature change negatively correlated with available phosphorus. In summary, exogenous substances input might lead to the accumulation of WSOC in urban green space, however, urban environment was helpful to maintain the stability of WSOC, which might be due to the enrichment of available phosphorus in urban sites.


Assuntos
Carbono/análise , Florestas , Pradaria , Solo/química , Temperatura , Água , China , Nitrogênio/análise , Fósforo/análise
14.
Yi Chuan ; 28(5): 533-9, 2006 May.
Artigo em Chinês | MEDLINE | ID: mdl-16735231

RESUMO

The chromosome samples of Rana nigromaculata were prepared by peripheral blood lymphocyte cultured in vitro. Karyotype, C-banding and silver-staining nucleolus organizer regions (Ag-NORs) were observed on chromosomes samples. The result showed that: (1) the chromosome number of Rana nigromaculata is 26 (2n=26), i.e., 10 large and 16 small chromosomes, the large chromosomes are matched into 5 homologous pairs and small chromosomes are matched into 8 homologous pairs, and further indicates the karyotype of Rana nigromaculata is bi-modal; (2) Metaphases of female and male were observed separately, the chromosome of No.11 has a pronounced secondary constriction in the middle of long arms. But the position of secondary constriction in the aberrant type individuals is in the middle of long arms of the No.8; (3) it has only one obvious C-banding close to the telomere, which is located in the long arms of the No.5; (4) The chromosome No.11 is a pair of homologous chromosomes with Ag-NORs, and the position of Ag-NORs in female and male is identical.


Assuntos
Região Organizadora do Nucléolo/genética , Ranidae/genética , Animais , Bandeamento Cromossômico , Feminino , Cariotipagem , Masculino , Coloração pela Prata
15.
Hereditas ; 143(2006): 130-7, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17362346

RESUMO

Here we describe our studies on Microtus mandarinus faeceus of Jiangyan in Jiangsu province of China. By karyotype and G-banding analysis we have found variation in chromosome number and polymorphisms of the X chromosome and the second pair of autosomes of the subspecies. Chromosome number of the subspecies is 2n=47-50. The subspecies has three kinds of chromosomal sex: XX, XO and XY, among which one of the X chromosomes is subtelocentric (X(ST)) and the other is metacentric (X(M)). After comparing karyotypes of different subspecies, we found the specific cytogenetic characteristics of Microtus mandarinus, that is they have three kinds of chromosomal sex: XX, XO and XY; X chromosomes are heteromorphic; the chromosome number of female individuals are one less than male individuals; chromosome number of XX individuals are equal to that of XO ones. We hypothesize that Robertsonian translocation is the main reason of the polymorphism of the second pair of autosomes and variety of chromosome number, and it also causes the chromosome number evolution in different subspecies of Microtus mandarinus.


Assuntos
Arvicolinae/genética , Cromossomos de Mamíferos , Variação Genética , Cromossomo X , Animais , Arvicolinae/classificação , Aberrações Cromossômicas , Bandeamento Cromossômico , Feminino , Gônadas/metabolismo , Masculino , Metáfase , Polimorfismo Genético , Aberrações dos Cromossomos Sexuais
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