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1.
Opt Lett ; 45(3): 706-709, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32004290

RESUMO

Mueller polarimetry is a powerful optical technique in the analysis of micro-structural properties of optical samples. However, there is no explicit relationship between individual Mueller matrix elements and the physical properties of the sample. Several matrix decomposition algorithms corresponding to specific optical models have been proposed, which extract the physical information from measured Mueller matrices. Nevertheless, we still need a prior assessment method to decide which model is more suitable for the experimental data. In this Letter, we propose a set of characteristic Mueller matrices that allows us to obtain information about the breaking of rotation, mirror, and reciprocal symmetry properties in the sample by direct inspection of several elements of the Mueller matrix. By further analyzing the possible origin of symmetry breaking, we can learn the type and mixing status of anisotropies in the measured sample. We have verified our theory with Monte Carlo simulations of polarized light scattering in an isotropic or anisotropic medium containing different configurations of spherical and cylindrical scatterers. This study may help experimenters choose more suitable Mueller matrix decomposition methods.

2.
EMBO Mol Med ; 12(2): e10154, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31943789

RESUMO

Diabetic retinopathy (DR) is a common complication of diabetes and leads to blindness. Anti-VEGF is a primary treatment for DR. Its therapeutic effect is limited in non- or poor responders despite frequent injections. By performing a comprehensive analysis of the semaphorins family, we identified the increased expression of Sema4D during oxygen-induced retinopathy (OIR) and streptozotocin (STZ)-induced retinopathy. The levels of soluble Sema4D (sSema4D) were significantly increased in the aqueous fluid of DR patients and correlated negatively with the success of anti-VEGF therapy during clinical follow-up. We found that Sema4D/PlexinB1 induced endothelial cell dysfunction via mDIA1, which was mediated through Src-dependent VE-cadherin dysfunction. Furthermore, genetic disruption of Sema4D/PlexinB1 or intravitreal injection of anti-Sema4D antibody reduced pericyte loss and vascular leakage in STZ model as well as alleviated neovascularization in OIR model. Moreover, anti-Sema4D had a therapeutic advantage over anti-VEGF on pericyte dysfunction. Anti-Sema4D and anti-VEGF also conferred a synergistic therapeutic effect in two DR models. Thus, this study indicates an alternative therapeutic strategy with anti-Sema4D to complement or improve the current treatment of DR.

3.
Int J Biol Macromol ; 148: 424-433, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31954122

RESUMO

Chitin was extracted from shrimp shells powders (SSP) by successive two-step fermentation. The best microorganisms Lactobacillus rhamnoides and Bacillus amyloliquefaciens (BA01) for demineralization (DM) and deproteinization (DP) were obtained and the optimal fermentation conditions for two-step fermentation were established. Firstly, we determined the cultured conditions (inoculum level 4%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 5%, cultured time 48 h) of Lactobacillus rhamnoides and the organic acid quantities and types of fermentation broth of Lactobacillus rhamnoides. Under the conditions, the pH of fermentation broth was 3.4, the DM efficiency was 97.5% and the ash in the final residue was 1.2%, and the main organic acid was lactic acid. Secondly, the optimal cultured conditions of BA01 were inoculum level 6%, initial pH 6.5, cultured temperature 37 °C, glucose concentration 4%, and cultured time 84 h. Under the conditions, the protease activity of fermentation broth was 701.3 U/mL, the DP efficiency was 96.8%, the protein in the final residue was 1.5%, and the chitin yield was 19.6%. In addition, the chitin obtained by fermentation was compared with the commercial chitin using scanning Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), Solid-state 13C CP/MAS-NMR spectra, and Scanning electron microscope (SEM). The results showed the chitin obtained by fermentation maintains the excellent physicochemical and structural properties of commercial chitin. Moreover, in order to make full use of shrimp and crab shells resources, the amino acid composition of fermentation broth was detected. The results showed that the fermentation broth had high nutritional value and could be used as a health nutrient in animal feed, even food.

4.
Cell Adh Migr ; 14(1): 1-11, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31910058

RESUMO

Reducted arylamine N-acetyltransferase (NAT1) in breast cancers is associated with poor patient survival. NAT1 has also been associated with changes in cancer cell survival and invasion both in vitro and in vivo. Here, we report the effects of NAT1 in cancer cell invasion by addressing its role in adherence, migration, and invasion in vitro. The NAT1 gene was deleted in MDA-MB-231, HT-29 and HeLa cells using CRISPR/Cas9 gene editing. Loss of NAT1 increased adherence to collagen in all three cell-lines but migration was unaffected. NAT1 deletion decreased invasion and induced changes to cell morphology. These effects were independent of matrix metalloproteinases but were related to integrin ITGαV expression. The data suggest NAT1 is important in adhesion and invasion through integrin expression.

5.
Int J Mol Sci ; 21(2)2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31941068

RESUMO

As a natural polysaccharide, chitosan possesses good biocompatibility, biodegradability and biosafety. Its hydroxyl and amino groups make it an ideal carrier material in the construction of polymer-drug conjugates. In recent years, various synthetic strategies have been used to couple chitosan with active substances to obtain conjugates with diverse structures and unique functions. In particular, chitosan conjugates with antimicrobial activity have shown great application prospects in the fields of medicine, food, and agriculture in recent years. Hence, we will place substantial emphasis on the synthetic approaches for preparing chitosan conjugates and their antimicrobial applications, which are not well summarized. Meanwhile, the challenges, limitations, and prospects of antimicrobial chitosan conjugates are described and discussed.

6.
Tree Physiol ; 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31976532

RESUMO

In traditional opinions, no radial transportation was considered to occur in the bamboo internodes but was usually considered to occur in the nodes. Few studies involved the phloem unloading and post-phloem transport pathways in the rapid elongating bamboo shoots. Our observations indicated a symplastic pathway in phloem unloading and post-unloading pathways in the culms of Fargesia yunnanensis, based on 5,6-carboxyfluorescein diacetate tracing experiment. Significant lignification and suberinization in fiber and parenchyma cell walls in maturing internodes, blocked the apoplastic transport. Assimilates were transported out of the vascular bundles in four directions in the inner zones but in two directions in the outer zones via the continuum of parenchyma cells. In transverse sections, assimilates were outward transported from the inner zones to the outer zones. Assimilates transport velocities varied with time, with the highest values at 0:00 a.m., which were affected by water transport. The assimilate transport from the the adult culms to the young shoots also varied with the developmental degrees of bamboo shoots, with the highest transport velocities in the rapidly elongating internodes. The localization of sucrose, glucose, starch grains and the related enzymes reconfirmed that the parenchyma cells in and around the vascular bundles constituted a symplastic pathway for the radial transport of sugars and were the main sites for sugar metabolism. The parenchyma cells functioned as the "rays" for the radial transport in and between vascular bundles in bamboo internodes. These results systematically revealed the transport mechanism of assimilate and water in the elongating bamboo shoots.

7.
J Biomed Opt ; 25(1): 1-11, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31933331

RESUMO

Significance: Definitive diagnostics of many diseases is based on the histological analysis of thin tissue cuts with optical white light microscopy. Extra information on tissue structural properties obtained with polarized light would help the pathologist to improve the accuracy of his diagnosis.

Aim: We report on using Mueller matrix microscopy data, logarithmic decomposition, and polarized Monte Carlo (MC) modeling for qualitative and quantitative analysis of thin tissue cuts to extract the information on tissue microstructure that is not available with a conventional white light microscopy.

Approach: Unstained cuts of human skin equivalents were measured with a custom-built liquid-crystal-based Mueller microscope in transmission configuration. To interpret experimental data, we performed the simulations with a polarized MC algorithm for scattering anisotropic media. Several optical models of tissue (spherical scatterers within birefringent host medium, and combination of spherical and cylindrical scatterers within either isotropic or birefringent host medium) were tested.

Results: A set of rotation invariants for the logarithmic decomposition of a Mueller matrix was derived to rule out the impact of sample orientation. These invariants were calculated for both simulated and measured Mueller matrices of the dermal layer of skin equivalents. We demonstrated that only the simulations with a model combining both spherical and cylindrical scatterers within birefringent host medium reproduced the experimental trends in optical properties of the dermal layer (linear retardance, linear dichroism, and anisotropic linear depolarization) with layer thickness.

Conclusions: Our studies prove that Mueller polarimetry provides relevant information not only on a size of dominant scatterers (e.g., cell nuclei versus subwavelength organelles) but also on its shape (e.g., cells versus collagen fibers). The latter is directly related to the state of extracellular collagen matrix, which is often affected by early pathology. Hence, using polarimetric data can help to increase the accuracy of diagnosis.

8.
Cell Signal ; 66: 109462, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31689506

RESUMO

Lung adenocarcinoma is the most common subtype of non-small-cell lung cancer affecting people all over the globe. Recent studies have indicated that long non-coding RNAs (lncRNAs) possess the ability to regulate gene expression. Initially, we uncovered increased LINC00355 expressions in lung adenocarcinoma tissues and cells. Functionally, our findings demonstrated that LINC00355 silencing suppressed the proliferation in vitro and in vivo. In addition, we found that LINC00355 negatively regulated miR-195 in lung adenocarcinoma cells. Simultaneously, silencing LINC00355 by shRNA resulted in suppressed proliferation, colony formation and promoted cell cycle arrest and apoptosis via miR-195. Moreover, silencing LINC00355 by shRNA inhibited the cyclin E1 (CCNE1) gene expression via miR-195 in lung adenocarcinoma cells. Collectively, this study demonstrates the novel lncRNA LINC00355 in regulatory network of CCNE1 via miR-195 in lung adenocarcinoma, highlighting LINC00355 as a new target for the treatment of lung adenocarcinoma.

9.
Plant Biotechnol J ; 18(1): 96-105, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31131506

RESUMO

Peanut (Arachis hypogaea. L) is an important oil crop worldwide. The common testa colours of peanut varieties are pink or red. But the peanut varieties with dark purple testa have been focused in recent years due to the potential high levels of anthocyanin, an added nutritional value of antioxidant. However, the genetic mechanism regulating testa colour of peanut is unknown. In this study, we found that the purple testa was decided by the female parent and controlled by a single major gene named AhTc1. To identify the candidate gene controlling peanut purple testa, whole-genome resequencing-based approach (QTL-seq) was applied, and a total of 260.9 Gb of data were generated from the parental and bulked lines. SNP index analysis indicated that AhTc1 located in a 4.7 Mb region in chromosome A10, which was confirmed by bulked segregant RNA sequencing (BSR) analysis in three segregation populations derived from the crosses between pink and purple testa varieties. Allele-specific markers were developed and demonstrated that the marker pTesta1089 was closely linked with purple testa. Further, AhTc1 encoding a R2R3-MYB gene was positional cloned. The expression of AhTc1 was significantly up-regulated in the purple testa parent YH29. Overexpression of AhTc1 in transgenic tobacco plants led to purple colour of leaves, flowers, pods and seeds. In conclusion, AhTc1, encoding a R2R3-MYB transcription factor and conferring peanut purple testa, was identified, which will be useful for peanut molecular breeding selection for cultivars with purple testa colour for potential increased nutritional value to consumers.

11.
J Cell Physiol ; 235(3): 2149-2160, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31389030

RESUMO

miR-222 participates in many cardiovascular diseases, but its effect on cardiac remodeling induced by diabetes is unclear. This study evaluated the functional role of miR-222 in cardiac fibrosis in diabetic mice. Streptozotocin (STZ) was used to establish a type 1 diabetic mouse model. After 10 weeks of STZ injection, mice were intravenously injected with Ad-miR-222 to induce the overexpression of miR-222. miR-222 overexpression reduced cardiac fibrosis and improved cardiac function in diabetic mice. Mechanistically, miR-222 inhibited the endothelium to mesenchymal transition (EndMT) in diabetic mouse hearts. Mouse heart fibroblasts and endothelial cells were isolated and cultured with high glucose (HG). An miR-222 mimic did not affect HG-induced fibroblast activation and function but did suppress the HG-induced EndMT process. The antagonism of miR-222 by antagomir inhibited HG-induced EndMT. miR-222 regulated the promoter region of ß-catenin, thus negatively regulating the Wnt/ß-catenin pathway, which was confirmed by ß-catenin siRNA. Taken together, our results indicated that miR-222 inhibited cardiac fibrosis in diabetic mice via negatively regulating Wnt/ß-catenin-mediated EndMT.

12.
Biosens Bioelectron ; 147: 111790, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31669805

RESUMO

In this study, modified hollow titanium dioxide and SnS2 quantum dots (SnS2 QDs) were used to build a novel electrochemiluminescence immunoassay (ECLIA) for ultrasensitive detection of chloramphenicol (CAP). The titanium dioxide hollow spheres (THS) modified with polyethyleneimine (PEI) and gold nanoparticles (Au NPs) was synthesized as a probe material for the novel ECLIA, meanwhile it also could be a coreaction accelerator which could greatly improve the interaction efficiency between SnS2 QDs and S2O82-. Then the modified THS was combined with chloramphenicol antibody to form ECLIA probes, and colloidal gold synthesized as a platform for an ECLIA by ascorbic acid (AA) reduction. A competitive immunoassay strategy was used for the detection of CAP, where CAP in the sample would compete with the coating antigen for the limited antibodies. The proposed ECLIA for CAP detection exhibited high sensitivity with a wide linear range from 0.01 ng mL-1 to 100 ng mL-1 and a low detection limit at 3.1 pg mL-1. Furthermore, it could be seen from electrochemical analysis that the effect of THS on the enhancement of ECL signal is about twice as high as that of ordinary titanium dioxide. Importantly, this work not only successfully applied THS to amplify electrochemical-luminescence signal in the ECLIA, but also successfully prepared a stable and highly selective sensor for ultrasensitive detection of CAP.

13.
Carbohydr Polym ; 229: 115423, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31826462

RESUMO

Chitosan (CS) and its water-soluble derivatives, hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and sulfated chitosan (SCS), were used as adjuvants of inactivated Newcastle disease (ND) vaccine. First, NDV-loaded and blank CS, HACC/CS and SCS nanoparticles were prepared. The particle sizes were respectively 343.43 ±â€¯4.12, 320.03 ±â€¯0.84, 156.2 ±â€¯9.29 nm and the zeta potentials were respectively +19.67 ±â€¯0.58, +18.3 ±â€¯0.5, -17.8 ±â€¯2.65 mV under the optimal conditions. Then chickens were immunized with nanoparticles or commercial inactivated oil emulsion vaccine. After immunization, the humoral immunity levels of the chickens were evaluated. The cellular immunity levels were determined by the quantification of cytokines, lymphocyte proliferation assay, the percentages of CD4+ and CD8+ T lymphocytes. Finally, the chickens were challenged with highly virulent virus. The results demonstrated that the humoral immunity levels in NDV-loaded CS and HACC/CS nanoparticles groups were lower than commercial vaccine but the cellular immunity levels are better. Moreover, the prevention effects of NDV-loaded CS and HACC/CS nanoparticles against highly virulent NDV are comparable to commercial vaccine. Our study provides the basis of developing HACC and CS as effective vaccine adjuvants.

14.
Heredity (Edinb) ; 124(1): 122-134, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31358987

RESUMO

Seed filling is a dynamic process that determines seed size and nutritional quality. This time-dependent trait follows a logistic (S-shaped) growth curve that can be described by a logistic function, with parameters of biological relevance. When compared between genotypes, the filling dynamics variations are explained by the differences of parameter values; as such, the parameter estimates can be considered as "traits" for genetic analysis to identify loci that are associated with the seed-filling process. We carried out genetic and genomic analysis of the seed-filling process in maize, using a recombinant inbred line (RIL) population derived from the two inbred lines with contrasting seed-filling dynamics. We recorded seed dry weight at 14 time points after pollination, spanning the early filling phases to the late maturation stages. Fitting these data to a logistic model allowed for estimating 12 characteristic parameters that can be used to meaningfully describe the seed-filling process. Quantitative trait locus (QTL) mapping of these parameters identified a total of 90 nonredundant loci. Using bulked segregant RNA-sequencing (BSR-seq) analysis, we identified eight genes that showed differential gene expression patterns at multiple time points between the extreme pools, and these genes co-localize with the mapped QTL regions. Two of the eight genes, GRMZM2G391936 and GRMZM2G008263, are implicated in starch and sucrose metabolism, and biosynthesis of secondary metabolites that are well known for playing a vital role in seed filling. This study suggests that the logistic model-based approach can efficiently identify genetic loci that regulate dynamic developing traits.

15.
Med Image Anal ; 59: 101561, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31671320

RESUMO

Diabetic Retinopathy (DR) is the most common cause of avoidable vision loss, predominantly affecting the working-age population across the globe. Screening for DR, coupled with timely consultation and treatment, is a globally trusted policy to avoid vision loss. However, implementation of DR screening programs is challenging due to the scarcity of medical professionals able to screen a growing global diabetic population at risk for DR. Computer-aided disease diagnosis in retinal image analysis could provide a sustainable approach for such large-scale screening effort. The recent scientific advances in computing capacity and machine learning approaches provide an avenue for biomedical scientists to reach this goal. Aiming to advance the state-of-the-art in automatic DR diagnosis, a grand challenge on "Diabetic Retinopathy - Segmentation and Grading" was organized in conjunction with the IEEE International Symposium on Biomedical Imaging (ISBI - 2018). In this paper, we report the set-up and results of this challenge that is primarily based on Indian Diabetic Retinopathy Image Dataset (IDRiD). There were three principal sub-challenges: lesion segmentation, disease severity grading, and localization of retinal landmarks and segmentation. These multiple tasks in this challenge allow to test the generalizability of algorithms, and this is what makes it different from existing ones. It received a positive response from the scientific community with 148 submissions from 495 registrations effectively entered in this challenge. This paper outlines the challenge, its organization, the dataset used, evaluation methods and results of top-performing participating solutions. The top-performing approaches utilized a blend of clinical information, data augmentation, and an ensemble of models. These findings have the potential to enable new developments in retinal image analysis and image-based DR screening in particular.

16.
Sci Rep ; 9(1): 17987, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31784630

RESUMO

Multi-atlas-based segmentation (MAS) methods have demonstrated superior performance in the field of automatic image segmentation, and label fusion is an important part of MAS methods. In this paper, we propose a label fusion method that incorporates pixel greyscale probability information. The proposed method combines the advantages of label fusion methods based on sparse representation (SRLF) and weighted voting methods using patch similarity weights (PSWV) and introduces pixel greyscale probability information to improve the segmentation accuracy. We apply the proposed method to the segmentation of deep brain tissues in challenging 3D brain MR images from publicly available IBSR datasets, including images of the thalamus, hippocampus, caudate, putamen, pallidum and amygdala. The experimental results show that the proposed method has higher segmentation accuracy and robustness than the related methods. Compared with the state-of-the-art methods, the proposed method obtains the best putamen, pallidum and amygdala segmentation results and hippocampus and caudate segmentation results that are similar to those of the comparison methods.

17.
Beilstein J Nanotechnol ; 10: 2251-2260, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31807410

RESUMO

We report a simple one-step hydrothermal strategy for the fabrication of a C-MoS2/rGO composite with both large surface area and high porosity for the use as advanced electrode material in lithium-sulfur batteries. Double modified defect-rich MoS2 nanosheets are successfully prepared by introducing reduced graphene oxide (rGO) and amorphous carbon. The conductibility of the cathodes can be improved through the combination of amorphous carbon and rGO, which could also limit the dissolution of polysulfides. After annealing at different temperatures, it is found that the C-MoS2/rGO-6-S composite annealed at 600 °C yields a noticeably enhanced performance of lithium-sulfur batteries, with a high specific capacity of 572 mAh·g-1 at 0.2C after 550 cycles, and 551 mAh·g-1 even at 2C, much better than that of MoS2-S nanosheets (249 mAh·g-1 and 149 mAh·g-1) and C-MoS2/rGO-S composites (334 mAh·g-1 and 382 mAh·g-1). Our intended electrode design protocol and annealing process may pave the way for the construction of other high-performance metal disulfide electrodes for electrochemical energy storage.

18.
Colloids Surf B Biointerfaces ; 186: 110674, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31855686

RESUMO

The design and development of advanced gene/drug codelivery nanocarrier with good biocompatibility for cancer gene therapy is desirable. Herein, we reported a gene delivery nanoplatform to synergized bortezomib (BTZ) for cancer treatment with histone H2A-hybrided, upconversion luminescence (UCL)-guided mesoporous silica nanoparticles [UCNPs(BTZ)@mSiO2-H2A]. The functionalization of H2A on the surface of UCNPs(BTZ)@mSiO2 nanoparticles realized the improvement of biocompatibility and enhancement of gene encapsulation and transfection efficiency. More importantly, then UCNPs(BTZ)@mSiO2-H2A/p53 induced specific and efficient apoptotic cell death in p53-null cancer cells and restored the functional activity of tumor suppressor p53 by the success of co-delivery of BTZ/p53. Moreover, the transfection with UCNPs(BTZ)@mSiO2-H2A/p53 in p53-deficient non-small cell lung cancer cells changed the status of p53 and substantially enhanced the p53-mediated sensitivity of encapsulated BTZ inside the UCNPs(BTZ)@mSiO2/p53. Meanwhile, core-shell structured mesoporous silica nanoparticles UCNPs@mSiO2 as an UCL agent can detect the real-time interaction of nanoparticles with cells and uptake/penetration processes. The results here suggested that the as-developed UCNPs(BTZ)@mSiO2-H2A/p53 nanoplatform with coordinating biocompatibility, UCL image, and sustained release manner might be desirable gene/drug codelivery nanocarrier for clinical cancer therapy.

19.
Opt Lett ; 44(23): 5812-5815, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31774786

RESUMO

Laser speckle contrast imaging maps the changes in blood flow by estimating the decorrelation time of a scattered light field. However, speckle contrast is a biased statistics estimator that results in a theoretic bias between its expected value and the true value. Moreover, the average of speckle contrast depends on the statistical sampling size, which further hinders the estimation of decorrelation time from speckle contrast. Here, we present a new, to the best of our knowledge, unbiased statistics analysis based on auto-inverse covariance to improve the estimation of decorrelation time using laser speckle. Theoretical and experimental results demonstrate that the speckle auto-inverse covariance analysis is mean-invariant, so that the average of the estimation is not dependent on the sampling size. Furthermore, it can produce less statistical fluctuation, especially for slow flow, and consume less computation time than that of speckle contrast analysis.

20.
Sensors (Basel) ; 19(23)2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31757030

RESUMO

Airborne LiDAR bathymetry (ALB) has shown great potential in shallow water and coastal mapping. However, due to the variability of the waveforms, it is hard to detect the signals from the received waveforms with a single algorithm. This study proposed a depth-adaptive waveform decomposition method to fit the waveforms of different depths with different models. In the proposed method, waveforms are divided into two categories based on the water depth, labeled as "shallow water (SW)" and "deep water (DW)". An empirical waveform model (EW) based on the calibration waveform is constructed for SW waveform decomposition which is more suitable than classical models, and an exponential function with second-order polynomial model (EFSP) is proposed for DW waveform decomposition which performs better than the quadrilateral model. In solving the model's parameters, a trust region algorithm is introduced to improve the probability of convergence. The proposed method is tested on two field datasets and two simulated datasets to assess the accuracy of the water surface detected in the shallow water and water bottom detected in the deep water. The experimental results show that, compared with the traditional methods, the proposed method performs best, with a high signal detection rate (99.11% in shallow water and 74.64% in deep water), low RMSE (0.09 m for water surface and 0.11 m for water bottom) and wide bathymetric range (0.22 m to 40.49 m).

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