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1.
Sci Total Environ ; 806(Pt 2): 150628, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34592294

RESUMO

Green space is associated with many health benefits, but evidence concerning the effects on chronic kidney disease (CKD) has not been investigated. Using the nationwide cross-sectional study of 47,204 adults from the China National Survey of Chronic Kidney Disease dataset and residential greenness assessed by the normalized difference vegetation index (NDVI), this study evaluated the association between residential greenness and CKD prevalence. An interquartile range increase in NDVI1000m (0.26) was associated with decreased odds of CKD for all participants with an odds ratio (OR) of 0.79 (95% confidence interval [CI]: 0.73-0.86). Subgroup analyses demonstrated more apparent inverse associations in younger adults <65 years, male participants, people in higher socio-economic status, as well as people with smoking and alcohol drinking habit. In addition, more apparent inverse associations were found in regions with higher fine particulate matter (PM2.5) concentration levels, with OR of 0.56 (95% CI: 0.49, 0.65) for higher pollution regions, and OR of 0.95 (95% CI: 0.83, 1.09) for lower pollution regions (P for interaction <0.001). The exposure-response curves captured more apparent declines in OR of CKD when in lower NDVI1000m exposure ranges (<0.6), even controlling for the PM2.5 concentration. Our results indicated that residential greenness might be beneficial for the prevention and control of CKD at the population level, suggesting the positive significance of strengthening green space construction, particularly in regions with low greenness.

2.
Bioengineered ; 12(2): 10982-10993, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34851228

RESUMO

To investigate the protective effects of metformin on the diabetic mice with cognitive impairment induced by the combination of streptozotocin (STZ) and isoflurane anesthesia. The isoflurane-anesthetized cognitive impairment model mice were established and then observed via behavioral tests and histopathological examination. Then these model mice were randomly assigned to three groups, which received the PBS, low and high doses of metformin, respectively. The body weight, food and water consumption of model mice were measured every other day. The mechanisms of metformin on ameliorating the cognitive dysfunction were further investigated by histomorphological, biochemical and Western blot analysis. After 14-days treatment of metformin, the diabetic symptoms in STZ-induced diabetic mice were significantly alleviated. Metformin could restore the isoflurane- and STZ-induced hippocampal tissue damage, cognitive and memory impairment in exposed space via improving the oxidative stress, upregulating the contents of glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) in the hippocampus tissues of diabetic mice. Furthermore, chronic treatment of metformin significantly down-regulated the expression of AGEs, RAGE, pNF-κB, iNOS, and COX-2. In conclusion, metformin can improve the isoflurane- and STZ-induced cognitive impairment in diabetic mice via improving oxidative stress and inhibiting the AGEs/RAGE/NF-κB signaling pathway.

3.
Bioengineered ; 2021 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-34747301

RESUMO

The discarding and burning of corn stalks in the fields after harvesting lead to environmental pollution and waste of resources. Composting is an effective way to disposal of the crop straws. Composting is a complex biochemical process and need a detail studied in cold region. Hence, the succession process of bacteria and Actinomycetes in the process of corn stalk composting in cold region was studied by 16SrRNA. Alpha diversity analysis showed that the detection results could represent the real situation. The bacterial community diversity from high to low was F50 > F90 > F0 > F10 > F20. The results of beta analysis showed that F20 and F50 had the most similar microbial structure at the phylum level, and the difference between F0 and F20 was the largest. The dominant microbes changed from Proteobacteria and Bacteroidetes in F0 in heating stage to Firmicutes and Proteobacteria, Actinobacteria and Firmicutes in F10 during early high temperature stage, and Actinobacteria, Proteobacteria and Bacteroidetes in cooling and post composting phases. Actinobacteria and Firmicutes were the dominant bacteria in the whole composting process. In the composting process, the microbial community was mainly involved in amino acid metabolism related to nitrogen transformation and carbohydrate metabolism related to lignocellulose degradation. Lignin and hemicellulose were mainly degraded in thermophilic stage. The conversion of nitrogen and degradation of cellulose occurred mainly in the early stages of composting. The research will be helpful to understand the biochemical process of composting in cold region.

4.
Environ Chem Lett ; : 1-11, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34744548

RESUMO

Despite large decreases of emissions of air pollution during the coronavirus disease 2019 (COVID-19) lockdown in 2020, an unexpected regional severe haze has still occurred over the North China Plain. To clarify the origin of this pollution, we studied air concentrations of fine particulate matter (PM2.5), NO2, O3, PM10, SO2, and CO in Beijing, Hengshui and Baoding during the lockdown period from January 24 to 29, 2020. Variations of PM2.5 composition in inorganic ions, elemental carbon and organic matter were also investigated. The HYSPLIT model was used to calculate backward trajectories and concentration weighted trajectories. Results of the cluster trajectory analysis and model simulations show that the severe haze was caused mainly by the emissions of northeastern non-stopping industries located in Inner Mongolia, Liaoning, Hebei, and Tianjin. In Beijing, Hengshui and Baoding, the mixing layer heights were about 30% lower and the maximum relative humidity was 83% higher than the annual averages, and the average wind speeds were lower than 1.5 m s-1. The concentrations of NO3 -, SO4 2-, NH4 +, organics and K+ were the main components of PM2.5 in Beijing and Hengshui, while organics, K+, NO3 -, SO4 2-, and NH4 + were the main components of PM2.5 in Baoding. Contrary to previous reports suggesting a southerly transport of air pollution, we found that northeast transport caused the haze formation. Supplementary Information: The online version contains supplementary material available at 10.1007/s10311-021-01314-8.

5.
J Pain Res ; 14: 3511-3517, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34785948

RESUMO

Background: Modic changes (MC) are generally considered to be related to degenerative disc disease, and there is no uniform standard for surgical methods for lumbar disc herniation (LDH) accompanied by Modic type I changes (MC I). The purpose of this study was to observe the clinical results of percutaneous transforaminal endoscopic decompression (PTED) and transforaminal lumbar interbody fusion (TLIF) for treatment of LDH accompanied by MC I. Methods: Of the 53 consecutive patients included, 29 underwent PTED and 24 underwent TLIF. All patients were followed up for at least 24 months. Preoperative demographic characteristics, perioperative outcomes, and clinical outcomes were recorded. Visual analog scale (VAS) scores, Oswestry disability index (ODI) scores, and modified Macnab criteria were used to assess clinical results. Results: The mean age was 53.7±9.2 years in the PTED group and 53.6±9.6 years in the TLIF group. The scores of VAS legs, VAS back and ODI in the two groups after operation were significantly improved compared with those before operation (P<0.05). Notably, the VAS back pain score and ODI in the PTED group showed an increasing trend with time. And the VAS back pain scores and ODI of the two groups were statistically different at 1 year and 2 years postoperatively (P<0.05). In addition, compared with the TLIF group, the PTED group showed less operation time, blood loss, and postoperative hospital stay (P<0.05). At the final follow-up, the excellent rates were 91.7% and 86.2% in the fusion and PTED groups, respectively. Conclusion: Both PTED and TLIF procedures significantly improved the clinical symptoms of single-level LDH patients with MC I. Compared with TLIF, MC I may affect the improvement of low back pain and functional status after PTED.

6.
Chemistry ; 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34792222

RESUMO

In recent years, molecular ferroelectrics have received great attention due to their lightweight, mechanical flexibility, easy preparation and excellent ferroelectric properties. Among them, crown ether-based molecular ferroelectrics, which are typically composed of the host crown ethers, the guest cations anchored in the crown ethers, and the counterions, are of great interest because of the host-guest structure. Such a structure allows the components to occur order-disorder transition easily, which is beneficial for inducing ferroelectric phase transition. Herein, we summarized the research progress of crown ether host-guest molecular ferroelectrics, focusing on their crystal structure, phase transition, ferroelectric-related properties. In view of the small spontaneous polarization and uniaxial nature, we outlook the chemical design strategies for obtaining high-performance crown ether-based molecular ferroelectrics. This minireview will be of guiding significance for the future exploration of crown ether host-guest molecular ferroelectrics.

7.
BMC Cardiovasc Disord ; 21(1): 554, 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34798829

RESUMO

BACKGROUND AND AIMS: Atherosclerosis is a vital cause of cardiovascular diseases. The correlation between proteinuria and atherosclerosis, however, has not been confirmed. This study aimed to assess whether there is a relationship between proteinuria and atherosclerosis. METHODS: From January 2016 to September 2020, 13,545 asymptomatic subjects from four centres in southern China underwent dipstick proteinuria testing and carotid atherosclerosis examination. Data on demography and past medical history were collected, and laboratory examinations were performed. The samples consisted of 7405 subjects (4875 males and 2530 females), excluding subjects failing to reach predefined standards and containing enough information. A multivariate logistic regression model was used to adjust the influence of traditional risk factors for atherosclerosis on the results. RESULTS: Compared with proteinuria-negative subjects, proteinuria-positive subjects had a higher prevalence rate of carotid atherosclerosis. The differences were statistically significant (22.6% vs. 26.7%, χ2 = 10.03, p = 0.002). After adjusting for common risk factors for atherosclerosis, age, sex, BMI, blood lipids, blood pressure, renal function, hypertensive disease, diabetes mellitus and hyperlipidaemia, proteinuria was an independent risk factor for atherosclerosis (OR = 1.191, 95% CI 1.015-1.398, p = 0.033). The Hosmer-Lemeshow test was used to test the risk prediction model of atherosclerosis, and the results showed that the model has high goodness of fit and strong independent variable prediction ability. CONCLUSIONS: Proteinuria is independently related to carotid atherosclerosis. With the increase in proteinuria level, the risk of carotid atherosclerotic plaque increases. For patients with positive proteinuria, further examination of atherosclerosis should not be ignored.

8.
Mitochondrial DNA B Resour ; 6(12): 3411-3412, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34805518

RESUMO

Dictyosquilla foveolata is a moderately important commercial species that is naturally distributed from Australia to Borneo, and from northwest Indonesia to Burma, Vietnam, and China. The complete mitogenome sequence of D. foveolata was first determined using Illumina HiSeq sequencing in this study. The circular genome was 15,733 bp in length and consisted of 13 protein-coding genes and 2 ribosomal RNA genes, 22 transfer RNA genes. The mitochondrial gene arrangement of D. foveolata is similar to those of most other stomatopods. Results from maximum-likelihood phylogenetic analysis showed that D. foveolata was clustered with other six species of the family Squillidae. This study will be valuable for phylogenetic analyses of the genus Dictyosquilla and the other genera of the order Stomatopoda. This is the first record of the complete mitogenome for the genus Dictyosquilla.

9.
J Orthop Surg Res ; 16(1): 676, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34789313

RESUMO

OBJECTIVE: To reveal noteworthy characteristics of intramedullary (IM) nail breakage in pertrochanteric femur fractures. MATERIALS AND METHODS: The data from 6 patients with IM nail breakage in our hospital between August 2008 and May 2018 were reviewed retrospectively. With an additional 64 cases reported in articles in the PubMed database prior to October 2019, a total of 70 cases were reviewed and analysed; epidemiological patient data and data on the initial trauma, fracture type, nail selected for the original surgery, time from surgery to breakage, mechanism and location of breakage, status of fracture healing, salvage treatment and prognosis were assessed. RESULTS: Seventy patients with pertrochanteric fractures, including 2 stable fractures and 68 unstable fractures, experienced IM nail breakage at a mean of 9.4 months after the initial surgery. Among them, 9 (12.9%) occurred within 3 months, 23 (32.9%) between 3 and 6 months and 38 (54.3%) after 6 months. The mean age was 72.3 years, and 16 (22.9%) patients were younger than 65 years old. When nail breakage occurred, 66 fractures (94.3%) exhibited delayed union/non-union. Self-dynamisation was found in 12 cases (17.1%). The salvage procedures included 4 partial/total implant removal procedures, 17 hemi/total hip arthroplasty procedures, 3 conservative treatment procedures, and 46 revised osteosyntheses, of which 7 cases (15.2%) sustained secondary implant failure. No significant differences were found between the failure rates of IM nails and extramedullary(EM) devices (odds ratio [OR], 3.429; 95% confidence interval [CI], 0.632-18.877; p = 0.330). CONCLUSION: IM nail breakage is a rare complication lack of time regularity and mostly occurs in unstable pertrochanteric fractures in the presence of delayed union/non-union. Osteosynthesis revision can be conduct by a new IM nail or EM device but considerable secondary failure rate is noteworthy. Self-dynamisation may be a warning sign of nail breakage.

10.
Comput Intell Neurosci ; 2021: 9996232, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34804153

RESUMO

The most advanced method for crowd counting uses a fully convolutional network that extracts image features and then generates a crowd density map. However, this process often encounters multiscale and contextual loss problems. To address these problems, we propose a multiscale aggregation network (MANet) that includes a feature extraction encoder (FEE) and a density map decoder (DMD). The FEE uses a cascaded scale pyramid network to extract multiscale features and obtains contextual features through dense connections. The DMD uses deconvolution and fusion operations to generate features containing detailed information. These features can be further converted into high-quality density maps to accurately calculate the number of people in a crowd. An empirical comparison using four mainstream datasets (ShanghaiTech, WorldExpo'10, UCF_CC_50, and SmartCity) shows that the proposed method is more effective in terms of the mean absolute error and mean squared error. The source code is available at https://github.com/lpfworld/MANet.


Assuntos
Processamento de Imagem Assistida por Computador , Redes Neurais de Computação , Humanos , Software
11.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 37(6): 688-693, 2021 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-34821107

RESUMO

Objective: To investigate the effects of different intensity of swimming training on p66Shc protein in mouse myocardium. Methods: Fifty Kunming mice were randomly divided into control group (Group C), weight-bearing swimming group (Group E), weight-bearing swimming + drug group (Group ER), non weight-bearing swimming group (Group P), non weight-bearing swimming + drug group (Group PR), with 10 mice in each group. Group C did not exercise. Groups E, ER, P, and PR received swimming training for 4 weeks. Groups E and ER performed weight-bearing swimming with a 3% body weight, and Group P and Group PR were swimming without weight-bearing, 60 min/d, 6 times/w. Mice in ER and PR groups were injected intraperitoneally with Rottlerin (0.3 mg/kg), a PKCδ inhibitor, before the last two exercises. Groups C, E, and P were injected with the same dose of normal saline. Samples were collected after training finished for 24 hours. The protein expressions of PKCδ, P-PKCδ, P66Shc, P-P66shc and NOX2 were detected by Western blot; PKCδ and P66Shc were detected by immunoprecipitation; malondialdehyde (MDA), reactive oxygen species (ROS) and superoxide dismutase (SOD) in myocardium and serum were analyzed by biochemistry. Results: Compared with Group C, the protein expressions of PKCδ, P-PKCδ, P66Shc, P-P66shc and NOX2 in Group E were increased significantly (P< 0.01), the serum and myocardial MDA levels, myocardial ROS were increased significantly (P<0.05 or P<0.01), and the myocardial SOD activity was decreased (P<0.01), the PKCδ, P-PKCδ, P-P66shc and NOX2 in Group P were increased significantly (P<0.05 or P<0.01), and the myocardial SOD activity was enhanced (P<0.05). Compared with Group E, the protein expressionS of PKCδ (P<0.01), P-PKCδ (P<0.01), P66Shc (P<0.05), P-P66shc (P<0.01), NOX2 (P<0.05) in Group ER was decreased significantly, the protein expression of P66Shc in Group P was decreased significantly (P<0.05), the myocardial MDA (P<0.01) and ROS (P<0.05) were decreased, and the activity of SOD was enhanced (P<0.01). Compared with Group P, the protein expressions of PKCδ, P-PKCδ and P-P66shc in Group PR were decreased significantly (P<0.01), while the expression of NOX2 was increased (P<0.05). Conclusion: Both swimming training of two intensities promoted the increase of PKCδ protein and its phosphorylation in mouse cardiomyocytes. High-intensity swimming training could significantly enhance the expression and phosphorylation level of p66Shc protein, resulting in the production of ROS and the decrease of antioxidant enzyme activity. Low-intensity swimming training enhanced the phosphorylation of p66Shc, but did not promote its protein expression, resulting in the enhancement of myocardial antioxidant capacity and exercise adaptation.


Assuntos
Estresse Oxidativo , Natação , Animais , Camundongos , Miocárdio/metabolismo , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src/metabolismo
12.
Dig Liver Dis ; 2021 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-34782279

RESUMO

BACKGROUND: Pseudogenes are dysfunctional copies of protein-coding genes that showed critical regulatory roles during carcinogenesis. Plasminogen like A (PLGLA) is a transcribed unprocessed pseudogene and biasedly expressed in liver. But its function has not been studied in hepatocellular carcinoma (HCC). AIMS: We aimed to explore the role of PLGLA in HCC. METHODS: The expression of PLGLA and its association with pathological features in HCC patients was analyzed using The Cancer Genome Atlas (TCGA) datasets. Quantitative reverse transcription PCR (qRT-PCR) was used to validate PLGLA level in HCC tissue samples and cell lines. Gain-of-function experiments in vitro and in vivo were employed to assess the impact of PLGLA on HCC cell proliferation, migration and invasion. Luciferase reporter assay and RNA pull-down assay were conducted to confirm the interaction among PLGLA, miR-324-3p and GLYATL1. RESULTS: PLGLA expression was significantly downregulated in HCC tissues and cell lines. Furthermore, low PLGLA expression was positively associated with tumor progression and poor prognosis. PLGLA restoration markedly suppressed cell proliferation, migration and invasion. Mechanistically, PLGLA could competitively bind to miR-324-3p and acted as a competitive endogenous RNA (ceRNA) to enhance GLYATL1 expression. CONCLUSIONS: Our results established a novel tumor suppressive role of PLGLA in HCC pathogenesis and highlighted its potential as a therapeutic target for HCC treatment.

13.
J Bionic Eng ; : 1-13, 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34840554

RESUMO

Nanofibers (NFs) have been widely used in tissue engineering such as wound healing. In this work, the antibacterial ZnO quantum dots (ZnO QDs) have been incorporated into the biocompatible poly (ε-caprolactone)/collagen (PCL/Col) fibrous scaffolds for wound healing. The as-fabricated PCL-Col/ZnO fibrous scaffolds exhibited good swelling, antibacterial activity, and biodegradation behaviors, which were beneficial for the applications as a wound dressing. Moreover, the PCL-Col/ZnO fibrous scaffolds showed excellent cytocompatibility for promoting cell proliferation. The resultant PCL-Col/ZnO fibrous scaffolds containing vascular endothelial growth factor (VEGF) also exhibited promoted wound-healing effect through promoting expression of transforming growth factor-ß (TGF-ß) and the vascular factor (CD31) in tissues in the early stages of wound healing. This new electrospun fibrous scaffolds with wound-healing promotion and antibacterial property should be convenient for treating wound healing. Supplementary Information: The online version contains supplementary material available at 10.1007/s42235-021-00115-7.

14.
One Health ; 13: 100350, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34841035

RESUMO

Hepatitis E virus (HEV) is an emerging zoonotic pathogen posing global health burden, and the concerns in Europe are tremendously growing. Pigs serve as a main reservoir, contributing to pork-related foodborne transmission. In this study, we aim to specifically simulate this foodborne transmission route and to assess potential interventions. We firstly established a dose-response relationship between the risk of transmission to human and the amount of ingested viruses. We further estimated the incidence of HEV infection specifically attributed to pork-related foodborne transmission in four representative European countries. Finally, we demonstrated a proof-of-concept of mitigating HEV transmission by implementing vaccination in human and pig populations. Our modeling approach bears essential implications for better understanding the transmission of pork-related foodborne HEV and for developing mitigation strategies.

15.
ACS Appl Mater Interfaces ; 13(40): 47478-47487, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34601863

RESUMO

Nanoporous graphenes (NPGs) have recently attracted huge attention owing to their designable structures and diverse properties. Many important properties of NPGs are determined by their structural regularity and homogeneity. The mass production of NPGs with periodic well-defined pore structures under a solvent-free green synthesis poses a great challenge and is largely unexplored. A facile synthetic strategy of NPGs via pressing organization calcination (POC) of readily available halogenated polycyclic aromatic hydrocarbons is developed. The gram-scale synthesized NPGs have ordered structures and possess well-defined nanopores, which can be easily exfoliated to few layers and oxidized in controllable approaches. After being decorated with oxygen species, the oxidized NPGs with tunable catalytic centers exhibit high activity, selectivity, and stability toward electrochemical hydrogen peroxide generation.

16.
Polymers (Basel) ; 13(20)2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34685240

RESUMO

Suction cups of cephalopods show a preeminent performance when absorbing irregular or flat objects. In this paper, an octopi-inspired suction cup, driven by hydraulically coupled dielectric elastomer actuators (HCDEAs), is proposed, which is considered to be controlled easily and have compact structure. To investigate the performance of suction cups, experiments have been conducted to clarify the effect of the pre-stretch ratio and chamber angle on suction forces. It could be seen that both factors have a complicated influence on suction forces, and the best performance obtained was a reasonable combination of the pre-stretch ratio and chamber angle. Here, we achieved a maximum suction force of 175 mN with λp = 1.2, α = 23° under a DC voltage of 3500 V. To enhance the capacity and adaptation of the suction cup, flat objects of various types of materials were introduced as targets. Experimental results displayed that for tested materials, including a dry/wet acrylic plate, CD, ceramic wafer, and aluminum plate, the suction cup showed outstanding performance of absorbing and lifting the target without any damage or scratch to them. Our research may serve as a guide to the optimal design and provide insights into the performance of the HCDEAs-actuated suction cup.

17.
Front Bioeng Biotechnol ; 9: 733980, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34692656

RESUMO

Delayed chest closure (DSC) is widely performed during the treatment of congenital heart diseases. However, the high prevalence of surgical site infection (SSI) in patients undergoing DSC affects prognosis negatively. Herein, we designed a suturable poly (vinyl alcohol)/keratin film loaded with silver nanoparticles (AgNPs) as an alternative material for DSC, which was named PVA/Keratin/AgNPs. The PVA/Keratin/AgNPs films exhibited significantly enhanced mechanical strength after crosslinking by sodium trimetaphosphate (STMP). These films were non-toxic, and cells proliferated with good morphology after 1 week of culture. In addition, PVA/Keratin/AgNPs films provided superior antibacterial ability, as evidenced by the eradication and lower growth rate of Staphylococcus aureus and Escherichia coli. Finally, the PVA/Keratin/AgNPs films were demonstrated to successfully cover the chest cavity temporarily and protect the chest cavity from bacterial infection. These results indicated that the PVA/Keratin/AgNPs films have great prospects to be further exploited for clinical applications in DSC.

18.
Bioengineered ; 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34699320

RESUMO

E2F family of transcription factors modulates multiple cellular functions associated with cell cycle and apoptosis. Here, we focused on the relevance of E2F1 to esophageal squamous cell carcinoma (ESCC) and identification of E2F1-mediated network in this study. Query of Gene Expression Omnibus database revealed that E2F1 was the core gene that was upregulated in ESCC. E2F1 downregulation inhibited ESCC cell activity. microRNA (miR)-375 was confirmed to be a downstream target of E2F1. E2F1 bound to miR-375 promoter and inhibited miR-375 transcription. Moreover, miR-375 inhibitor mitigated the repressive impacts of si-E2F1 on ESCC cells in part. Further study showed that sestrin 3 (SESN3) could interact with miR-375, and its knockdown annulled the stimulative effect of miR-375 inhibitor on ESCC development. Finally, E2F1 and SESN3 downregulation inhibited the phosphatidylinositol 3 kinase (PI3K)/AKT pathway activity in cells, while miR-375 inhibitor promoted PI3K/AKT pathway activation. These findings suggest that E2F1 inhibited miR-375 expression and promoted SESN3 expression to activate the PI3K/AKT pathway in ESCC.

19.
Mol Med Rep ; 24(6)2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34643246

RESUMO

DL­3­n­butylphthalide (NBP) and 3­methyl­1- phenyl­2­pyrazolin­5­one (edaravone) are acknowledged neuroprotective agents that protect against ischemic stroke. However, the underlying mechanisms of a combination therapy with NBP and edaravone have not yet been fully clarified. The aim of the present study was to explore whether the co­administration of NBP and edaravone had multi­target protective effects on the neurovascular unit (NVU) of mice affected by ischemic stroke. Male C57BL/6 mice were randomly divided into the following three groups: i) Sham operation control, ii) middle cerebral artery occlusion (MCAO) and reperfusion, iii) and MCAO/reperfusion with the co­administration of NBP (40 mg/kg) and edaravone (6 mg/kg) delivered via intraperitoneal injection at 0 and 4 h after reperfusion (NBP + edaravone). After ischemia and reperfusion, infarct volumes and neurological deficits were evaluated. The immunoreactivity of the NVU, comprising neurons, endothelial cells and astrocytes, was determined using immunofluorescence staining of neuronal nuclei (NeuN), platelet and endothelial cell adhesion molecule 1 (CD31) and glial fibrillary acidic protein (GFAP). Western blotting was used to detect the expression levels of apoptosis­related proteins. The infarct volume, neurological function scores and cell damage were increased in the MCAO group compared with the sham operation group. Furthermore, the MCAO mice had reduced NeuN and CD31 expression and increased GFAP expression compared with the sham group. By contrast, the NBP + edaravone group exhibited reduced cell damage and consequently lower infarct volume and neurological deficit scores compared with the MCAO group. The NBP + edaravone group exhibited increased NeuN and CD31 expression and decreased GFAP expression compared with the MCAO group. Furthermore, the expression levels of Bax and cleaved caspase­3 in the NBP + edaravone group were decreased significantly compared with the MCAO group, while the expression levels of Bcl­2 and mitochondrial cytochrome c were increased. In conclusion, the results of the present study demonstrated that NBP and edaravone effectively prevented ischemic stroke damage with multi­target protective effects. In addition, NBP + edaravone may be a promising combination therapy for ischemic stroke.

20.
Soft Robot ; 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34705563

RESUMO

Compared with traditional rigid grippers, soft grippers are made of lightweight and soft materials and have the characteristics of flexible contact and strong adaptability, which are widely utilized to grasp fragile objects with complex contours and shapes. In this article, we design and fabricate a three-fingered stiffness-tunable soft gripper by integrating the joint-tuning capability. The soft fingers are composed of an internal bending actuator and an external fiber-jamming jacket, under an actuation of pneumatic pressure. Static and kinematic models are established to detect the bending angle and end trajectory of the internal bending actuator. Meanwhile, the bending angle and blocking force of bending actuator are experimentally measured and are comparably analyzed with the theoretical predictions. Jamming pressure is applied in the stiffness-tunable jacket to explore the variable stiffness and load-carrying capability of the soft finger. By incorporating the stiffness-tunable property, the grasping performance of various weights and types of goods, as well as the maximum grasping force of the soft gripper, is investigated. Finally, by patterning the stiffness-tunable jacket on the bending actuator, the variable curvature bending deformation and joint-tuning capability of the soft finger are achieved. This proposed soft gripper holds great potential applications in soft robotics community.

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