Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 230
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Nanosci Nanotechnol ; 20(2): 852-857, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383080

RESUMO

To provide clean energies, great efforts have been contributed to hydrogen production via photocatalytic water splitting over the past decades. Among many potential materials, TiO2 nanotube array has been considered as one of the most attractive photoanodes due to its advantages, such as low cost, non-toxicity and chemical stability. It has been discovered that TiO2 nanotube arrays (TNTs)' photocatalytic performance was dictated by their morphologies, which is mainly controlled by the outer voltage. In order to further understand the effect of TNTs' morphologies on their photocatalytic capability, TNTs with different structures had been fabricated under different voltage (10, 20, 30, 40 and 50 V). The obtained TNTs' morphologies and phase were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM) and X-ray diffraction analyzer (XRD). The photocatalytic properties of TNTs were investigated in aqueous solution under xenon lamp irradiation. It was identified that the highest hydrogen production of the sample synthesized at the voltage of 30 V was 1979.8 µmol/h·g during the first cycle.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31668965

RESUMO

BACKGROUND: Based on the traditional Chinese medicine (TCM) theory, Renshen Yangrong Tang (RSYRT), which mixed with 12 herbs, commonly used as a pharmacological option in China for fatigue management by correcting Qi deficiency. This randomized controlled phase II trial investigated the efficacy of RSYRT for reducing cancer-related fatigue. METHODS: Cancer survivors with moderate or severe fatigue (rated ≥4 on a 0-10 scale) over 2 months were randomized to take herbal extract granules of RSYRT or a low dose of a single herb (huangqi) twice a day for 6 weeks. Patient-reported fatigue was measured using the MD Anderson Symptom Inventory (MDASI). Efficacy of RSYRT was evaluated using mixed model to test the differences over time among groups. We also conducted responder analyses and examined time to effect of symptom reduction. RESULTS: None of the 83 evaluable patients (control group, 42; intervention group, 41) had discomfort or grade 3 or 4 toxicity. We observed a significantly greater MDASI-fatigue score reduction in the intervention group than in the control group (time-by-group interaction, estimate (est) =-0.61(0.10), P<.0001). More patients in the intervention group had a 2-points reduction on fatigue than the control group (90.2% vs. 52.4%). By week 4, between groups difference of fatigue reduction on mean severity reached large effect size (intervention group vs. control group: -2.66 vs. -1.36, Cohen's d=1.0, P<0.0001). CONCLUSION: Compared with control therapy, RSYRT therapy elicits a statistical and clinical improvement of fatigue severity and functioning. RSYRT's effectiveness in managing cancer-related fatigue warrant further study in the real world.

3.
J Atheroscler Thromb ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31597886

RESUMO

AIM: Exosome-derived microRNAs (miRNAs) are potential diagnostic biomarkers. However, little is known about their effectiveness as diagnostic biomarkers of intracranial aneurysms (IAs). This study aimed to explore miRNA levels in plasma exosomes of patients with IA to identify potential biomarkers that predict the development and progress of IA. METHODS: A total of 69 patients with IA and 30 healthy controls (HC) were recruited, among whom 30 had unruptured IA (UA), and 39 had ruptured IA (RA). The miRNA expression profiles of plasma exosomes in 12 IA patients (4 UA and 8 RA) and 4 HC were determined using next-generation sequencing. In addition, significantly differentially expressed miRNAs were further analyzed by Quantitative Real-Time PCR (qRT-PCR) in a validation cohort of 99 subjects. RESULTS: From the sequencing analysis, 181 miRNAs were identified to be differently (p<0.05) expressed. Of these, 9 miRNAs were up-regulated, and 20 were down-regulated in patients with UA compared with HC. Also, 21 were up-regulated, and 10 were down-regulated in patients with RA compared with HC. In addition, compared with UA, 92 miRNAs were up-regulated in RA, whereas 29 were down-regulated. Furthermore, qRT-PCR analysis confirmed that miR-145-5p and miR-29a-3p were up-regulated in IA samples. To distinguish IA patients from controls, the area under the receiver operating characteristic curve was 0.791 for miR-29a-3p, while that of miRNA-145-5p was 0.773 in terms of discriminating whether the aneurysm was ruptured. CONCLUSIONS: Circulating exosomal miRNAs can serve as biomarkers of the development and progression of IA.

4.
Cell Mol Immunol ; 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31611650

RESUMO

Although PD-L1/PD-1 blockade therapy has been approved to treat many types of cancers, the majority of patients with solid tumors do not respond well, but the underlying reason remains unclear. Here, we studied ovarian cancer (OvCa), a tumor type generally resistant to current immunotherapies, to investigate PD-1-independent immunosuppression. We found that PD-L1 was not highly expressed in the tumor microenvironment (TME) of human OvCa. Instead, B7-H3, another checkpoint molecule, was highly expressed by both tumor cells and tumor-infiltrating antigen-presenting cells (APCs), which correlated with T-cell exhaustion in patients. Using ID8 OvCa mouse models, we found that B7-H3 expressed on tumor cells, but not host cells, had a dominant role in suppressing antitumor immunity. Therapeutically, B7-H3 blockade, but not PD-1 blockade, prolonged the survival of ID8 tumor-bearing mice. Collectively, our results demonstrate that tumor-expressed B7-H3 inhibits the function of CD8+ T cells and suggest that B7-H3 may be a target in patients who are not responsive to PD-L1/PD-1 inhibition, particularly OvCa patients.

5.
J Transl Med ; 17(1): 317, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31547822

RESUMO

BACKGROUND: Infertility has become a global health issue with the number of couples seeking in vitro fertilization (IVF) worldwide continuing to rise. Some couples remain childless after several IVF cycles. Women undergoing IVF face greater risks and financial burden. A prediction model to predict the live birth chance prior to the first IVF treatment is needed in clinical practice for patients counselling and shaping expectations. METHODS: Clinical data of 7188 women who underwent their first IVF treatment at the Reproductive Medical Center of Shengjing Hospital of China Medical University during 2014-2018 were retrospectively collected. Machine-learning based models were developed on 70% of the dataset using pre-treatment variables, and prediction performances were evaluated on the remaining 30% using receiver operating characteristic (ROC) analysis and calibration plot. Nested cross-validation was used to make an unbiased estimate of the generalization performance of the machine learning algorithms. RESULTS: The XGBoost model achieved an area under the ROC curve of 0.73 on the validation dataset and showed the best calibration compared with other machine learning algorithms. Nested cross-validation resulted in an average accuracy score of 0.70 ± 0.003 for the XGBoost model. CONCLUSIONS: A prediction model based on XGBoost was developed using age, AMH, BMI, duration of infertility, previous live birth, previous miscarriage, previous abortion and type of infertility as predictors. This study might be a promising step to provide personalized estimates of the cumulative live birth chance of the first complete IVF cycle before treatment.

6.
Ecotoxicol Environ Saf ; 184: 109610, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31522058

RESUMO

Montmorillonite (G-Mt) modified by a gemini quaternary ammonium cationic surfactant (Propyl bis (hexadecyl dimethyl ammonium) chloride, 16-3-16) was used to remove emerging contaminants (ECs) (such as 1H-Benzotriazole (BTA), 5-Methyl-1H-benzotriazole (TTA) and 1-Hydroxybenzotriazole (HOBT)) and Cu2+ from wastewater. Based on the adsorption of the above three ECs in our previous studies, single adsorption of Cu2+ and the simultaneous adsorption of three ECs with Cu2+ on G-Mt were also investigated. G-Mt showed much lower adsorption amount on Cu2+ comparing with original montmorillonite (Ca-Mt) in single adsorption system due to the difficulty of ion-exchange property of G-Mt. In co-adsorption system, three organic pollutants and Cu2+ played a synergistic effect and the adsorption capacity of G-Mt on them increased, the influence sequence of Cu2+ on the adsorption of three ECs or the effect of ECs on the adsorption of Cu2+ both followed as: TTA > BTA > HOBT. The results of FT-IR, EDS and XPS revealed that the complex of Cu2+ and ECs were adsorbed onto G-Mt via forming complexes and hydrophobic interaction in co-adsorption system. The pH experiment showed that the optimum pH of the co-adsorption of ECs and Cu2+ on G-Mt was 5. Molecular dynamics (MD) simulations showed that three ECs or ECs combining with Cu2+ were dominantly adsorbed in the interlayer space of G-Mt, which resulted in the arrangement manner of 16-3-16 between the layer of G-Mt before and after adsorption of three organic pollutants was different. Furthermore, by quantitatively analyzing electrostatic potential (ESP) distribution, average local ionization energy (ALIE) distribution and their minimum points on three ECs molecules surfaces, Multiwfn program has been applied to probe the microscopic mechanism. The synergistic effect of co-adsorption will promote enrichment of copper ions and ECs to remove them more efficiently in polluted waters.

7.
8.
Immunity ; 51(3): 522-534.e7, 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31471107

RESUMO

Although recent progress provides mechanistic insights into the pathogenesis of pulmonary fibrosis (PF), rare anti-PF therapeutics show definitive promise for treating this disease. Repeated lung epithelial injury results in injury-repairing response and inflammation, which drive the development of PF. Here, we report that chronic lung injury inactivated the ubiquitin-editing enzyme A20, causing progressive accumulation of the transcription factor C/EBPß in alveolar macrophages (AMs) from PF patients and mice, which upregulated a number of immunosuppressive and profibrotic factors promoting PF development. In response to chronic lung injury, elevated glycogen synthase kinase-3ß (GSK-3ß) interacted with and phosphorylated A20 to suppress C/EBPß degradation. Ectopic expression of A20 or pharmacological restoration of A20 activity by disturbing the A20-GSK-3ß interaction accelerated C/EBPß degradation and showed potent therapeutic efficacy against experimental PF. Our study indicates that a regulatory mechanism of the GSK-3ß-A20-C/EBPß axis in AMs may be a potential target for treating PF and fibroproliferative lung diseases.

9.
Reprod Toxicol ; 89: 130-135, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31306769

RESUMO

To assess the associations between air pollution and the pregnancy rate of IVF, we performed a retrospective cohort study on 1455 women who underwent the first fresh IVF cycle at a fertility center from January 2014 to April 2018 and lived in Shenyang during treatment. Binary logistic regression models were used to evaluate the effect of an interquartile range increase in each pollutant on the intrauterine pregnancy rate. Both average chronic and daily acute exposure to air pollution were considered. Increased O3 (adjusted OR = 0.87, 95% CI: 0.81, 0.98) during the period from gonadotropins starting to oocyte retrieval was associated with decreased probability of intrauterine pregnancy, especially the day before oocyte retrieval (adjusted OR = 0.86, 95% CI: 0.78, 0.95). Our results suggest that exposure to O3 before oocyte retrieval has an adverse effect on intrauterine pregnancy. Future studies should be performed in order to confirm our findings.

10.
Clin Exp Pharmacol Physiol ; 46(10): 937-943, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31271664

RESUMO

Abnormal expression or activity of proteins that regulate cell polarity can contribute to tumour progression. Discs large homolog (DLG) proteins play crucial roles in the maintenance of cell polarity and tissue morphogenesis. Previous studies of breast cancer patients showed that DLG3 had greater expression in the cancerous tissues than non-cancerous tissues, but the relationship between DLG3 expression and breast cancer progression and prognosis is not clear. Here, we investigated the association of DLG3 expression with breast cancer progression and prognosis using data on clinicopathological parameters from The Cancer Genome Atlas (TCGA) database, with different clinicopathological parameters using ualcan and LinkedOmics, and with different stages and subtypes using immunohistochemical staining. The results indicated greater DLG3 expression in cancerous breast tissues than normal breast tissues and in luminal and Her2+ subtypes than in the triple-negative subtype. DLG3 expression also had a positive correlation with pathologic stage and decreased survival rate. Our data suggest that DLG3 should be considered as a new diagnostic and prognostic biomarker for breast cancer.

11.
Biomed Chromatogr ; 33(11): e4638, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31261446

RESUMO

Monitoring gefitinib and its metabolites may help to explore the underlying mechanisms of gefitinib resistance. The concentration of gefitinib and its metabolites in tumor tissues could influence its anticancer activities more than that in the plasma. In the present study, a rapid and specific HPLC-MS/MS method was developed and validated to simultaneously determine gefitinib, M387783, M523595, M537194 and M608236 in tumor tissues of H1975 human lung cancer xenografts of nude mice. The established HPLC-MS/MS method was validated for specificity, linearity, accuracy and precision, matrix effect and recovery, carryover and dilution integrity, and analyte stability. The standard curves were linear (r2 ≥ 0.99) over the range of 0.5-100 ng/mL for M608236 and 1-200 ng/mL for gefitinib, M523595 and M537194 as well as M387783. The accuracy ranged from -8.35 to 6.03% relative error; and the precision was <15% relative standard deviation. Recoveries (87.74-99.96%) and matrix effects (86.60-106.40%) were satisfactory in the biological matrix examined. Stability studies showed that the analytes were stable during the assay procedure and storage. Finally, the validated method was successfully applied to study the pharmacokinetics profiles for gefitinib and its metabolites in nonsmall cell lung cancer (NSCLC) xenograft mouse tumors. Meanwhile, MTT assay showed that gefitinib had a more powerful inhibitory effect than its four major metabolites in H1975 NSCLC cells. This validated HPLC-MS/MS method may be applied to help understand the mechanisms of gefitinib resistance in EGFR-mutant nonsmall cell lung cancer.

13.
Mol Biol Rep ; 46(4): 4377-4383, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31147860

RESUMO

Our previous study revealed that CRB3 protein expression was reduced in clear cell renal cell carcinoma (ccRCC) and was associated with TNM stage, pathological grade, and poor prognosis of ccRCC. This study aimed to investigate if DNA methylation of CRB3 decreases its expression, subsequently leading to the progression and poor prognosis of ccRCC. Data for DNA methylation of CRB3, CRB3 mRNA expression, and ccRCC clinicopathological parameters were extracted from the cancer genome atlas (TCGA) database. The relationships among DNA methylation of CRB3, CRB3 mRNA expression, and ccRCC clinicopathological parameters were analyzed using UALCAN, MethHC, LinkedOmics, and Wanderer. We found that CRB3 mRNA levels were lower in ccRCC compared to normal tissues. Methylation of CRB3 increased in ccRCC, with all probes showing differences between ccRCC and normal tissues. Furthermore, CRB3 DNA methylation negatively correlated with CRB3 mRNA expression. CRB3 DNA methylation was also related to pathologic stage, T stage, N stage, and M stage of ccRCC. Overall survival was shorter in ccRCC patients with high CRB3 DNA methylation compared to ccRCC patients with low CRB3 DNA methylation. Methylation of cg24798010, a CRB3 probe, was related to laterality, pathologic stage, T stage, M stage, neoplasm-histologic-grade without N stage, and race. Furthermore, treatment with the DNA methylation inhibitor Decitabine resulted in the upregulation of CRB3 mRNA in ccRCC cell lines. These results indicate that DNA methylation of CRB3 may be both a prognostic marker and therapeutic target for ccRCC.

14.
Cell Res ; 29(7): 516-532, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31201384

RESUMO

Long-range communication between intestinal symbiotic bacteria and extra-intestinal organs can occur through circulating bacterial signal molecules, through neural circuits, or through cytokines or hormones from host cells. Here we report that Nod1 ligands derived from intestinal bacteria act as signal molecules and directly modulate insulin trafficking in pancreatic beta cells. The cytosolic peptidoglycan receptor Nod1 and its downstream adapter Rip2 are required for insulin trafficking in beta cells in a cell-autonomous manner. Mechanistically, upon recognizing cognate ligands, Nod1 and Rip2 localize to insulin vesicles, recruiting Rab1a to direct insulin trafficking through the cytoplasm. Importantly, intestinal lysozyme liberates Nod1 ligands into the circulation, thus enabling long-range communication between intestinal microbes and islets. The intestine-islet crosstalk bridged by Nod1 ligands modulates host glucose tolerance. Our study defines a new type of inter-organ communication based on circulating bacterial signal molecules, which has broad implications for understanding the mutualistic relationship between microbes and host.

15.
Toxins (Basel) ; 11(5)2019 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-31137632

RESUMO

Fusarium graminearum is a notorious pathogen that causes Fusarium head blight (FHB) in cereal crops. It produces secondary metabolites, such as deoxynivalenol, diminishing grain quality and leading to lesser crop yield. Many strategies have been developed to combat this pathogenic fungus; however, considering the lack of resistant cultivars and likelihood of environmental hazards upon using chemical pesticides, efforts have shifted toward the biocontrol of plant diseases, which is a sustainable and eco-friendly approach. Fengycin, derived from Bacillus amyloliquefaciens FZB42, was purified from the crude extract by HPLC and further analyzed by MALDI-TOF-MS. Its application resulted in structural deformations in fungal hyphae, as observed via scanning electron microscopy. In planta experiment revealed the ability of fengycin to suppress F. graminearum growth and highlighted its capacity to combat disease incidence. Fengycin significantly suppressed F. graminearum, and also reduced the deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), and zearalenone (ZEN) production in infected grains. To conclude, we report that fengycin produced by B. amyloliquefaciens FZB42 has potential as a biocontrol agent against F. graminearum and can also inhibit the mycotoxins produced by this fungus.

16.
Clin Exp Pharmacol Physiol ; 46(9): 837-844, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31087799

RESUMO

The crumbs protein homolog 3 (CRB3) regulates the tight junction to help maintain epithelial polarity. Altered CRB3 expression was associated with carcinogenesis of epithelial cells. This study detected CRB3 expression in 192 cases of breast cancer tissues and in the Molecular Taxonomy of Breast Cancer International Consortium (Metabric) and The Cancer Genome Atlas (TCGA) datasets for association with triple negative breast cancer (TNBC) phenotypes. The in vitro experiments confirm the ex vivo data. The data showed that levels of both CRB3 mRNA and protein were associated with TNBC phenotypes, ie, 41.1% (39/95) of ER+ breast cancer was CRB3-positive, whereas 26.9% (25/93) ER- tumour was CRB3-positive (P = 0.046). Moreover, 47.6% (30/63) of PR+ breast cancer was CRB3-positive vs 28.4% (33/116) PR- tumours positive for CRB3 (P = 0.013). In addition, 40.1% (27/66) of ER+/PR+ tumour was CRB3-positive, but only 22.4% (19/85) of TNBC showed CRB3 expression (P = 0.048). Indeed, levels of CRB3 mRNA were higher in non-TNBC than TNBC in both Metabric (P = 3.682e-10) and TCGA datasets (P = 2.501e-07). The in vitro data showed that CRB3 expression was higher in luminal (MCF7 and T47D) than in HER2 (MDA-MB-453 and SK-BR-3) and basal (MDA-MB-231 and BT-549) breast cancer cell lines. More interestingly, ERα regulated expression of CRB3 protein in MCF7 and BT-549 cells and ERα expression was associated with CRB3 expression in breast cancer tissues specimens. This study demonstrated that ERα could be a novel regulator for CRB3 expression in breast cancer.

17.
J Ethnopharmacol ; 238: 111847, 2019 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-30946966

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Marsdenia tenacissima is a traditional Chinese medicine that is known to be effective in combating cancer as well as reducing blood pressure. The efficacy and mechanisms of Marsdenia tenacissima in treating cancer have been well described. However, the potential vasoactivities of Marsdenia tenacissima remain poorly known. AIM OF THE STUDY: To determine the vasoactive effects of the water-soluble part of marsdenia tenacissima in mesenteric resistance arteries of the mice, and to explore the underlying mechanisms. MATERIALS AND METHODS: Isometric vessel tension study was used to examine the effects of marsdenia tenacissima extract (MTE) on vasodilation of the mesenteric arteries of mice. KCl, phenylephrine (PE) and 9,11-Dideoxy-11α,9α-epoxymethanoprostaglandin F2α (U46619) were used as vasoconstrictors. Y27632, Nitro-L-arginine methyl ester hydrochloride (L-NAME) and indomethacin were used to explore the underlying mechanisms for the vasoactivities of MTE. Western blot and nitric oxide (NO) assay were used to evaluate the effects of MTE on the activities of endothelial nitric oxide synthase (eNOS). RESULTS: MTE (5-50 mg/mL), but not vehicle, dose-dependently relaxed the mesenteric arteries constricted with KCl, PE or U46619, in which relaxations to KCl were more pronounced than that to PE or U46619. Pre-incubation of the vessels with MTE (40 mg/mL) reduced the vasoconstrictions caused by calcium influx. Decreasing calcium sensitivity by inhibition of Rho kinase (ROCK) significantly augmented the vasorelaxation of MTE. While, inhibition of endothelial cells by pre-incubation with L-NAME (300 µM) and indomethacin (10 µM) or denudating endothelial cells attenuated vasorelaxations of MTE to KCl, and with a larger potency, to U46619. In both human umbilical vein endothelial cells (HUVECs) and human heart microvascular endothelial cells (HMECs), the phosphorylations of eNOS and the production of NO were significantly enhanced after treatment of MTE for 2, 5, 10, 30 min. CONCLUSIONS: MTE, the water-soluble part of marsdenia tenacissima, was effective in relaxing mesenteric resistance arteries via inhibiting calcium influx and stimulating eNOS activities.

18.
ACS Appl Mater Interfaces ; 11(14): 13624-13631, 2019 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-30888141

RESUMO

Combining polymeric materials and conductive one-dimensional metal nanostructures is able to achieve enhanced chemical and electrical properties, but the control over their morphology and spatial arrangement remains a big challenge. Herein, by replacing benzenedicarboxylate (BDC) in ZnBDC nanoplates with oleylamine (OAM) in the presence of HAuCl4, Zn-OAM nanobelts with a highly ordered laminar structure were obtained, on which ultrathin Au nanowires (Au NWs) were deposited and aligned along the long axes of the nanobelts. The resulting Zn-OAM/Au NW hybrid further underwent an OAM-to-2-methylimidazole ligand exchange, resulting in the formation of porous nanobelts composed of ZIF-8 nanocrystals interwound with aligned Au NWs. Due to the synergistic effect between the polymeric and metallic structures, the Zn-OAM/Au NW hybrid nanobelts and ZIF-8/Au NW porous nanobelts demonstrated fast and selective gas sensing at ambient conditions, in sharp contrast to the nonresponsive Au NWs or Zn-based polymers alone.

19.
Neuron ; 101(3): 375-379, 2019 02 06.
Artigo em Inglês | MEDLINE | ID: mdl-30731061

RESUMO

The China Brain Project is in development. Integrating an ethical framework to identify and assess ethical challenges and plan for solutions is a priority. Here Wang et al. discuss ethical questions emerging from brain research in the context of traditional Chinese culture and juxtapose the legacy of Confucianism with contemporary thinking.


Assuntos
Características Culturais , Neurociências/ética , Encéfalo/fisiologia , China , Confucionismo , Humanos , Neurociências/normas
20.
World Neurosurg ; 125: 327-328, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30771547

RESUMO

Penetrating brain injury is 1 type of traumatic brain injury. Brain abscess is a common complication after penetrating brain injury, and fragments increase the risk of occurrence of brain abscess. It is uncommon to see the migration of bone fragments in the brain in clinical cases. We report a rare case of brain abscess with migration of bone fragments after traumatic brain injury.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA