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1.
Sensors (Basel) ; 20(3)2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31973131

RESUMO

The application of inertial measurement units (IMU) in electronically power-assisted cycles (EPACs) has become increasingly important for improving their functionalities. One central issue of such an application is to calibrate the orientation of the IMU on the EPAC. The approach presented in this paper utilizes common bicycling motions to calibrate the 2D- and 3D-mounting orientation of a micro-electro-mechanical system (MEMS) IMU on an electric bicycle. The method is independent of sensor biases and requires only a very low computation expense and, thus, the estimation can be realized in real-time. In addition, the acceleration biases are estimated using a barometric pressure sensor. The experimental results show high accuracy of the calibrated orientation and estimated sensor biases.

2.
Nanotechnology ; 31(13): 135603, 2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-31816613

RESUMO

In this work, Ag-Cu and Ag-Sn nanoparticles (NPs) were synthesized by a physical vapor condensation method, i.e. DC arc-discharge plasma. The as-prepared bimetallic NPs consist of metallic cores of Ag-Cu or Ag-Sn and ultrathin oxide shells of CuO or a hybrid of SnO and SnO2. Ag-Sn NPs exhibit a room-temperature resistivity of 4.24 × 10-5 Ω · cm, a little lower than 7.10 × 10-5 Ω · cm of Ag-Cu NPs. Both bimetallic NPs demonstrate typical metallic conduction behavior with a positive temperature coefficient of resistance over 25-300 K. Ag-Sn NPs exhibit thermally competitive stability up to 230 °C and a lower resistivity of 3.18 × 10-5 Ω · cm after sintering at 200 °C, giving it potential for application in flexible printed electronics.

3.
J Hazard Mater ; 385: 121557, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-31735469

RESUMO

Ceramic membranes allow a high filtration flux with a low fouling propensity. Direct filtration of municipal wastewater using flat-sheet ceramic membranes (FSCM) is an attractive and promising technology for wastewater treatment and resource recovery. Urgent need is to determine the fouling behavior of FSCM and its optimal cleaning strategy in direct filtration applications. This study was conducted on pre-coagulation of sewage with FeCl3 (20 mg Fe/L) or polyaluminum chloride (15 mg Al/L), followed by FSCM filtration at around 1.0 m/d (as high as 41.7 L/m2-h in flux). The results showed that the coagulation-FSCM filtration removed chemical oxygen demand and total phosphorus by up to 90.0% and 99.9%, respectively. The pollutants (organics and nutrients) in the sewage influent were concentrated into the sludge for potential resource recovery. Without pre-coagulation, foulants easily entered the membrane pores, causing rapid membrane fouling. Chemically enhanced coagulation greatly decreased the membrane fouling rate by aggregating various foulants into flocs. Fouled membranes were effectively renewed by physical means, such as water flushing and sonication, or a short period of chemical backwashing with NaOH, HCl or oxidizing agents. The study revealed the fouling mechanisms of FSCM in sewage filtration and developed effective cleaning strategies for its long-term operation.

4.
Int Urol Nephrol ; 52(1): 35-40, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31552576

RESUMO

BACKGROUND: Laparoscopic partial nephrectomy (LPN) with segmental renal artery clamping has become an important method to minimize the warm ischemia of the kidney during the surgery. In the present study, we adopted a new model of calculating surgery-related kidney volume loss (SKVL), which was derived from the imaging technology to predict the outcomes of LPN with segmental renal artery clamping. METHODS: A total of 111 consecutive patients underwent LPN with available pre- and post-operation computed tomography (CT) scanning data were retrospectively analyzed. The SKVL was calculated using the parameter derived from the CT scan. The correlation between the SKVL and the perioperative outcomes as well as the renal function loss was estimated by the logistic regression analyses. RESULTS: The mean SKVL was 8.99 cm3; kidney volume and tumor volume was 147.48 cm3 and 25.87 cm3, respectively. The SKVL was associated with maximum diameter of tumor (P = 0.001), tumor volume (P < 0.001), intraoperative blood loss (P < 0.001), and the warm ischemia time (P = 0.004), but not associated with the surgery time (P = 0.322) and complications (P = 0.638). Besides, the SKVL was associated with the renal function loss after LPN (P < 0.001). The multivariable logistic regression showed that SKVL was an independent parameter to predict the renal function loss. CONCLUSIONS: SKVL is a pre-operation parameter derived from the imaging data, which may be used to predict the perioperative outcomes and renal function loss of patients undergoing LPN.

5.
Int J Oncol ; 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31793654

RESUMO

Hepatitis B virus (HBV) has been revealed to be involved in the development of hepatocellular carcinoma. However, the mechanism remains to be fully elucidated. Smad­interacting protein 1 (SIP1) is a transcriptional repressor, which serves a pivotal role in cell metastasis. In the present study, the role of SIP1 in HBx­induced hepatocyte EMT and cancer aggressiveness was examined. It was found that HBV X protein (HBx) increased the expression of SIP1 and recruited it to the promoter of E­cadherin, resulting in depression of the transcription of E­cadherin. Histone deacetylase 1 was also found to be involved in the repressive complex formation. Furthermore, in an orthotopic tumor transplantation model in vivo, HBx promoted tumor growth and metastasis, whereas the knockdown of SIP1 attenuated the effect of HBx. These results indicate a novel mechanism for the development of HBV­related liver cancer.

6.
Huan Jing Ke Xue ; 40(6): 2533-2539, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854643

RESUMO

There are few analyses on the components of particulate matter emitted from waste incineration plants. In past studies, analyses of particle size distribution characteristics of the components were mainly targeted at particles with larger particle sizes. An electrical low pressure impactor (ELPI) was used in this study to collect the particulate matter emitted from a waste incineration plant, and the elements and carbonaceous components of these samples were analyzed. The particle size characteristics of organic carbon (OC), elemental carbon (EC), and heavy metal elements in 14 particle size segments were analyzed and composition profiles of elements and carbonaceous components of PM1, PM2.5, and PM10 from the waste incineration plant were established to provide a reference for refined source apportionment research. The results showed that the main components of the waste incineration plant included Al, Si, S, Ca, Cr, Fe, OC, EC, etc. OC and Ca were dominating components, and mass fractions of these components in the PM2.5 profile were 10.15% and 12.37%, respectively. The contents of heavy metals were ranked as Cr > Pb > Zn > Mn > Cu > Cd > Ni, and the mass fractions of Cr and Pb in PM2.5 amounted to 1.83% and 0.74%, respectively. OC in the range of 2.39-3.99 and 6.68-9.91 µm accounted for 15.02% and 20.45% of the total OC content, respectively, and the content of OC in fine particles was higher than that in coarse particles. The content of EC in fine particles was much higher than that in coarse particles, and it accounted for 14.8% in the 0.382-0.613 µm particle size. Heavy metal elements such as Cr, Mn, Ni, Cu, Zn, Cd, and Pb were mainly concentrated in the fine particles.

7.
Lasers Med Sci ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31768690

RESUMO

To evaluate the efficacy and safety of holmium laser enucleation of prostate (HoLEP) for treating urinary retention in patients with advanced prostate cancer. Thirty-eight cases of advanced prostate cancer with urinary retention were enrolled in this retrospective study. All the 38 patients were treated with CAB as a basis. Among them, 21 cases chose HoLEP additionally (HoLEP group). Seventeen cases stuck to CAB alone (CAB group). Serum PSA level, International Prostate Symptom Score (IPSS), quality of life score (QoLs), maximal flow rate (Qmax), and post-void residual volume (PVR) at 3, 6, 12, and 18 months after treatment were comparatively analyzed. The perioperative and postoperative parameters of HoLEP were assessed. Both groups demonstrated significant improvement in IPSS, QoLs, Qmax, and PVR during follow-up. But these parameters of HoLEP group improved more rapidly, significantly, and durably than CAB group. No serious complications were observed during and after HoLEP. PSA level of patients in both groups declined dramatically after surgery. But PSA in HoLEP group showed more dramatic and continuous drop. Besides, 1 of 21 patients in HoLEP group transferred into castration-resistant prostate cancer (CRPC) at 18th month of follow-up. While in CAB group, 5 of 17 patients developed into CRPC at 12th month of follow-up (P = 0.02 < 0.05). HoLEP was minimally invasive, safe, and effective, and could serve as a palliative approach to rapidly restore the patients' urine and play a cytoreductive role in advanced PCa to improve the oncological prognosis.

8.
Sci Rep ; 9(1): 15839, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31676878

RESUMO

Crop production, including mushroom farming, may cause significant changes to the underlying substrates which in turn, can influence crop quality and quantity during subsequent years. Here in this study, we analyzed the production of the medicinal mushroom Ganoderma lingzhi and the associated soil microbial communities and soil chemical features over 24 months from April 2015 to April 2017. This Basidiomycete mushroom, known as Lingzhi in China, is commonly found on dead trees and wood logs in temperate and subtropical forests. Its economic and medicinal importance have propelled the development of a diversity of cultivation methods. The dominant method uses wood logs as the main substrate, which after colonization by Lingzhi mycelia, are buried in the soil to induce fruiting. The soil microbial communities over the 24 months were analyzed using the Illumina HiSeq platform targeting a portion of the bacterial 16S rRNA gene and the fungal internal transcribed spacer 1 (ITS1). Overall, a significant reduction of Lingzhi yield was observed over our experimentation period. Interestingly, temporal changes in soil microbial compositions were detected during the 24 months, with the fungal community showing more changes than that of bacteria in terms of both species richness and the relative abundance of several dominant species after each fruiting. The soil chemical features also showed significant changes, with decreasing soil nitrogen and phosphorus concentrations and increasing soil pH and iron content after each fruiting. We discuss the implications of our results in sustainable Lingzhi production in soil.

9.
Anal Chim Acta ; 1092: 102-107, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31708022

RESUMO

Platelet-derived growth factor-BB (PDGF-BB) is currently used as a biomarker protein for cancer early diagnosis and clinical treatment. Herein, we reported a robust and enzyme-free strategy based on aptamer recognition and proximity-induced entropy-driven circuits (AR-PEDC) for homogeneous and rapid detection of platelet-derived growth factor BB (PDGF-BB) without any washing steps or thermocycling. The proximity probes specifically recognize target protein to form the completed trigger (CT). Then, the CT reacts with three-strand complex to form intermediate, which subsequently binds to fuel strand to release reporter strand, assistant strand and the CT. The revised proximity probes exhibit significantly improved signal-to-background ratio and faster association rate. Moreover, target protein/proximity probes interaction can specifically initiate entropy-driven circuits, thus providing immense signal amplification for ultrasensitive detection of PDGF-BB with low detection limit of 9.6 pM. The practical ability of the developed strategy is demonstrated by detection of PDGF-BB in human serum with satisfactory results. In addition, this method is flexible and can be conveniently extended to a variety of targets by simply substituting the target specific sequence. Thus, this strategy presents a rapid, low background and versatile amplification mechanism for the detection of protein biomarkers and offers a promising alternative platform for clinical diagnosis.

10.
Opt Lett ; 44(22): 5566-5569, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31730110

RESUMO

The quantum random number generation exploits inherent randomness of quantum mechanical processes and measurements. The real-time generation rate of quantum random numbers is usually limited by electronic bandwidth and data processing rates. Here we use a multiplexing scheme to create a fast real-time quantum random number generator based on continuous variable vacuum fluctuations. Multiple sideband frequency modes of a quantum vacuum state within a homodyne detection bandwidth are concurrently extracted as the randomness source. Parallel postprocessing of raw data from three subentropy sources is realized in one field-programmable gate array (FPGA) based on Toeplitz-hashing extractors. A cumulative generation rate of 8.25 Gbps in real time is achieved. The system relies on optoelectronic components and circuits that could be integrated in a compact, economical package.

11.
Cell Death Discov ; 5: 139, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31583122

RESUMO

Prostate cancer (PC) castration resistance has been linked to the differentiation of PC luminal cells into hormone-refractory neuroendocrine (NE) cells. However, the molecular mechanisms controlling the emergence of lethal NE prostate cancer (NEPC) remain unclear. The present study aimed to investigate the mechanisms underlying the transition from prostate adenocarcinoma to NEPC. The microRNA miR-708 was involved in NE differentiation and was downregulated in NEPC cells and tumor specimens. miR-708 targeted Sestrin-3 to inhibit Forkhead Box O1 (FOXO1) phosphorylation, resulting in apoptosis of prostate adenocarcinoma cells and AKT-inactivated NEPC cells, the latter of which was consistent with the progression of tumor xenografts in mice under miR-708 treatment. In silico analysis of PC and NEPC tumor specimens suggested that the polycomb repressive complex subunit Enhancer of zeste homolog 2 (EZH2) was particularly overexpressed in NEPC. Notably, EZH2 bound to the miR-708 promoter and induced its silencing in NEPC. Inhibition of EZH2 prevented NE differentiation of PC cells. EZH2 expression was regulated by both Cyclin Dependent Kinase 1 (CDK1) and Wnt signaling. Silencing transcription factor 4 (TCF4), as a key protein in Wnt signaling, prevented NEPC formation. These results provide a molecular basis for the roles of miR-708 and EZH2 in NE differentiation in PC and highlight a new paradigm in NEPC formation and survival.

12.
J Cell Mol Med ; 23(11): 7709-7716, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31557403

RESUMO

Inositol polyphosphate-1-phosphatase (INPP1) is an enzyme that is responsible for glycolysis and lipid metabolism. Here, we discovered that INPP1 expression was up-regulated in CC tissues compared to that in adjacent normal tissues by RT-qPCR. Inositol polyphosphate-1-phosphatase overexpression promoted and INPP1 knockdown suppressed cell viability, cellular migration/invasion and EMT in CC cells. To explore the mechanism of dysregulation, INPP1 was predicted to be a target of miR-27a, and a pmiRGLO dual-luciferase reporter assay showed that miR-27a bound to the 3' UTR of INPP1. RT-qPCR revealed that miR-27a was also up-regulated and had a positive correlation with INPP1 expression in CC tissues. Furthermore, shR-INPP1 could favour the malignant phenotype reversion induced by miR-27a, suggesting that miR-27a up-regulates INPP1 to promote tumorigenic activities. Altogether, our findings show that the up-regulation of INPP1 by miR-27a contributes to tumorigenic activities and may provide a potential biomarker for CC.

13.
Materials (Basel) ; 12(19)2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31547536

RESUMO

In aircraft engineering, an increase of internal pressure in a hydraulic pipe increases the probability of pipe damage, leading to crack propagation becoming a serious issue. In this study, the extended finite element method (XFEM) is applied to simulate initial crack propagation in hydraulic pipes and to investigate the influence factors. Stress intensity factors are extracted to verify the mesh independence of XFEM, which is based on the level set method and unit decomposition method. A total of 30 finite element models of hydraulic pipes with cracks are established. The distribution of von Mises stress under different initial crack lengths and internal pressures is obtained to analyze the change of load-carrying capacity in different conditions. Then, a total of 300 finite element models of hydraulic pipes with different initial crack sizes and locations are simulated under different working conditions. The relationship between the maximum opening displacement and crack length is analyzed by extracting the opening displacement under different initial crack lengths. The length and depth of the initial crack are changed to analyze the factors affecting crack propagation. The opening size and crack propagation length are obtained in different directions. The results show that radial propagation is more destructive than longitudinal propagation for hydraulic pipes in the initial stage of crack propagation.

14.
Cell Death Dis ; 10(9): 636, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31474757

RESUMO

microRNAs (miRNAs) play an important role in carcinogenesis. Typically, miRNAs downregulate the target expression by binding to the 3' UTR of mRNAs. However, recent studies have demonstrated that miRNAs can upregulate target gene expression, but its mechanism is not fully understood. We previously found that G-rich RNA sequence binding protein (GRSF1) mediates upregulation of miR-346 on hTERT gene. To explore whether GRSF1 mediate other miRNA's upregulation on their target genes, we obtained profile of GRSF1-bound miRNAs by Flag-GRSF1-RIP-deep sequencing and found 12 novel miRNAs, named miR-G. In this study, we focused on miR-G-10, which is highly expressed in cervical cancer tissues and cell lines and serum from patients with metastatic cervical cancer. miR-G-10 in cervical cancer cells significantly promoted migration/invasion and anoikis resistance in vitro and lung metastasis in vivo. Furthermore, miR-G-10 bound to the 3' UTR of PIK3R3 and upregulated its expression to activate the AKT/NF-κB signal pathway in a GRSF1-dependent manner, whereas miR-G-10 suppressed TIMP3 in the AGO2 complex to modulate the MMP9 signaling pathway in cervical cancer cells. Taken together, our findings may provide a new insight into the upregulation mechanism mediated by miRNAs and a potential biomarker for cervical cancer.

15.
Sci Total Environ ; 690: 911-922, 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31302555

RESUMO

In the saline-affected ecosystem, fungi have huge potential to promote growth, induce disease resistance and enhance tolerance against salt-stress of host plants. Since areas of plowland are gradually decreasing, the reclamation of coastal saline lands could play a crucial role in maintaining agricultural productivity and crop security globally. Therefore, it is of great significance to explore the fungal diversity in the coastal saline ecosystem. Here, we collected saline soil samples from unflooded areas and tidal flat areas, the two typical distinct landforms in coastal saline ecosystems, and used ITS metabarcoding to depict the diversity of fungal communities. We found that fungal species evenness had a remarkably higher variation from the tidal flat compared to unflooded soil samples. Furthermore, we also confirmed that the fungal niches differentiation reports in the coastal saline ecosystem. Our ITS based DNA sequencing revealed that both unflooded and tidal flat soil were mainly composed of amplicon sequence variants (ASVs) belonging to Ascomycota (93.43% and 86.91% respectively). Based on our findings, understanding the associations and distinctions of fungal microbiome between unflooded soil and tidal flat could provide the basis for the development of reclamation in coastal saline lands.


Assuntos
Código de Barras de DNA Taxonômico , Ecossistema , Fungos/genética , Microbiologia do Solo , Agricultura , Ascomicetos , Biodiversidade , DNA Fúngico , Fungos/classificação , Micobioma , Nitrogênio , Plantas , Solo
16.
J Mol Neurosci ; 69(1): 39-48, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31321646

RESUMO

Neurosyphilis is a chronic central nervous system infectious disease caused by Treponema pallidum. Our aim was to study the metabolic profiling in the cerebrospinal fluid of neurosyphilis patients and identify specific potential biomarkers. Fifteen cerebrospinal fluid samples from neurosyphilis patients and 14 non-neurosyphilis samples were analyzed by liquid chromatography-mass spectrometer (LC-MS). The LC-MS data were preprocessed by supervised pattern recognition to obtain diagnostic models. Both orthogonal projections to a latent structures discriminant analysis (OPLS-DA) and a t test were used to obtain specific metabolites for neurosyphilis. LC-MS data showed that the metabolites in cerebrospinal fluid (CSF) from neurosyphilis are different from the non-neurosyphilis group. The OPLS-DA model parameters R2Y and Q2Y are both more than 0.7 and indicated a satisfactory diagnostic performance. Bilirubin, L-histidine, prostaglandin E2, alpha-kamlolenic acid, and butyryl-L-carnitine and palmitoyl-L-carnitine were identified as novel potential biomarkers for neurosyphilis. The metabolic study of CSF may provide a new way to explore the pathogenesis of neurosyphilis.


Assuntos
Metaboloma , Neurossífilis/líquido cefalorraquidiano , Adulto , Bilirrubina/líquido cefalorraquidiano , Biomarcadores/líquido cefalorraquidiano , Carnitina/análogos & derivados , Carnitina/líquido cefalorraquidiano , Dinoprostona/líquido cefalorraquidiano , Ácidos Graxos Insaturados/líquido cefalorraquidiano , Feminino , Histidina/líquido cefalorraquidiano , Humanos , Masculino , Pessoa de Meia-Idade , Palmitoilcarnitina/líquido cefalorraquidiano
17.
Molecules ; 24(14)2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31315269

RESUMO

Mussel adhesive proteins (MAPs) have a unique ability to firmly adhere to different surfaces in aqueous environments via the special amino acid, 3,4-dihydroxyphenylalanine (DOPA). The catechol groups in DOPA are a key group for adhesive proteins, which is highly informative for the biomedical domain. By simulating MAPs, medical products can be developed for tissue adhesion, drug delivery, and wound healing. Hydrogel is a common formulation that is highly adaptable to numerous medical applications. Based on a discussion of the adhesion mechanism of MAPs, this paper reviews the formation and adhesion mechanism of catechol-functionalized hydrogels, types of hydrogels and main factors affecting adhesion, and medical applications of hydrogels, and future the development of catechol-functionalized hydrogels.


Assuntos
Bivalves/química , Catecóis/química , Animais , Bivalves/metabolismo , Di-Hidroxifenilalanina/química , Sistemas de Liberação de Medicamentos , Hidrogéis , Proteínas/metabolismo , Aderências Teciduais , Cicatrização
18.
Opt Express ; 27(13): 17859-17867, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31252738

RESUMO

We demonstrate experimentally that flat and broadband chaotic signals can be easily generated by combining a multi-mode laser diode subject to optical feedback with a band-pass filter. Measurements are made of the RF spectra of multi-mode and single-mode outputs from an external cavity Fabry-Perot (FP) semiconductor laser before and after the filtering procedure. In this way it is found that in the chaos regime the low-frequency energy of the single-mode output is enhanced by about 25 dB comparing with that of the multi-mode output. Moreover, the associated 3-dB chaos bandwidth can reach around 6 GHz for the single mode case. Numerical demonstrations show mode competition is the physical origin of the low-frequency enhancement in the single-mode chaotic outputs.

19.
Clin Biochem ; 73: 35-43, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31247187

RESUMO

BACKGROUND: Cardiac troponin (cTn) is a complex of three subunits (T, I, and C), with some studies reporting that ~5-10% is cytosolic and unbound ('free'). It has been hypothesized that free cTn is released before complex and before or without cell death dependent on the severity of ischemia. In this context, new generation assays that can discriminate free, binary (IC) and ternary (TIC) complex forms may aid to differentiate between type 1 myocardial infarction (MI) and cTn elevations due to different etiologies, e.g. demand ischemia and type 2 MI. METHODS: Serial plasma samples from six type 1 MI patients and twenty-seven patients with other cTnI elevations, e.g. due to demand ischemia and type 2 MI, were analyzed using high-sensitivity ET Healthcare Pylon assays for total cTnI, complex cTnI (IC and TIC), and cTnTIC. The specificity of the anti-cTnT antibody in the cTnTIC assay was such that only full-size cTnTIC is detected. In vitro stability of different cTnI forms was assessed by spiking free cTnI and cTnTIC in cTnI-free serum, incubating at 4 or 37 °C, and measuring different cTnI forms over 0-182 h. Presence of cytosolic free cTnI was evaluated on fixed rat cardiac tissue using an antibody against free cTnI. RESULTS: Pylon assays for total and complex cTnI tracked well over time with each other and gave similar results, both for type 1 MI and non-type 1 MI patients, indicating that the vast majority was complex cTnI. As a minority of complex cTnI was full-size cTnTIC, this indicated that complex cTnI mainly consisted of a degraded form of cTnTIC (low-molecular weight cTnTIC) and/or cTnIC. Full-size cTnTIC was more abundant in early compared to late samples. In vitro studies indicated that free cTnI and cTnTIC are not stable at 37 °C (28% and 11% recovery after 24 h, respectively) and this is also true to some extent for cTnTIC at 4 °C (60% recovery after 24 h). Free cTnI was not readily detected in rat cardiac tissue. CONCLUSIONS: In agreement with type 1 MI, cTnI in samples of patients with cTnI elevations due to other etiologies is found predominantly as complex cTnI, of which some is full-size cTnTIC. In most cases, assays for total and complex cTnI indicated there was little free cTnI; however, its presence cannot be completely excluded, due to the inability of its direct measurement and limited stability.


Assuntos
Infarto do Miocárdio/sangue , Miocárdio/metabolismo , Troponina I/sangue , Troponina T/sangue , Animais , Linhagem Celular , Feminino , Humanos , Masculino , Infarto do Miocárdio/classificação , Ratos , Ratos Nus
20.
Microbiologyopen ; 8(11): e871, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31251470

RESUMO

The emerging pollutants antibiotic resistance genes (ARGs) are prevalent in aquatic environments such as estuary. Coastal mangrove ecosystems always serve as natural wetlands for receiving sewage which always carry ARGs. Currently, the research considering ARG distribution in mangrove ecosystems gains more interest. In this work, we investigated the diversity of ARGs in an urban estuary containing mangrove and nonmangrove areas of the South China Sea. A total of 163 ARGs that classified into 22 resistance types and six resistance mechanisms were found. ARG abundance of the samples in the estuary is between 0.144 and 0.203. This is within the general range of Chinese estuaries. The difference analysis showed that abundances of total ARGs, six most abundant ARGs (mtrA, rpoB, rpoC, rpsL, ef-Tu, and parY), the most abundant resistance types (elfamycin, multidrug, and peptide), and the most abundant resistance mechanism (target alteration) were significantly lower in mangrove sediment than that in nonmangrove sediment (p < 0.05). Network and partial redundancy analysis showed that sediment properties and mobile genetic elements were the most influential factors impacting ARG distribution rather than microbial community. The two factors collectively explain 51.22% of the differences of ARG distribution. Our study indicated that mangrove sediments have the capacity to remove ARGs. This work provides a research paradigm for analysis of ARG prevalence and proliferation in the subtropical marine coastal mangrove ecosystem.

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