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1.
PeerJ ; 7: e7023, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31198641

RESUMO

Background: Rheumatoid arthritis (RA) is a chronic and nonspecific autoimmune disease, which leads to joint destruction and deformity. To investigate the potential of human mesenchymal stem cells (MSCs) as a new therapeutic strategy for patients with RA, we compared the therapeutic effects of bone marrow derived MSCs (BMSCs), umbilical cord derived MSCs (UCs), and stem cells derived from human exfoliated deciduous teeth (SHED) on collagen-induced arthritis (CIA) in mice. Methods: A total of 24 DBA/1 mice were infused with type II collagen to induce RA in the experimental model. MSC-treated mice were infused with UCs, BMSCs, and SHED, respectively. Bone erosion and joint destruction were measured by micro-computed tomographic (micro-CT) analysis and hematoxylin and eosin staining. The levels of tumor necrosis factor α (TNF-α) and interleukin-1ß (IL-1ß) were measured by immunohistochemistry and Enzyme-Linked Immunosorbent Assay (ELISA). Results: Systemic delivery of MSCs significantly improved the severity of the symptoms related to CIA to greater extent compared with the untreated control group. Micro-CT revealed reduced bone erosions in the metatarsophalangeal joints upon treatment with MSCs. Additionally, according to histologic evaluation, reduced synovitis and articular destruction were observed in MSC-treated groups. The levels of TNF-α and IL-1ß in the serum and joints decreased with treatment by MSCs. Conclusion: Our findings suggest that systemic infusion of UCs, BMSCs, and SHED may significantly alleviate the effects of RA. The therapeutic effect of BMSCs was greater than that of SHED, while the UCs were shown to have the best therapeutic effect on CIA mice. In conclusion, compared with BMSCs and SHED, UCs may be a more suitable source of MSCs for the treatment of patients with RA.

2.
J Mech Behav Biomed Mater ; 90: 86-95, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30359856

RESUMO

Premixed calcium phosphate cements (CPCs) have been developed to shorten the surgical time of conventional CPCs. However, there is lack of investigation on degradation behavior of premixed CPCs in vitro and in vivo. In this study, the premixed CPCs are prepared by mixing glycerol or polyethylene glycol (PEG) with the CPC power (ß-tricalcium phosphate (ß-TCP) and monocalcium phosphate monohydrate (MCPM)), and their degradation performances including the microstructure, chemical composition and mechanical properties are systematically evaluated both in vitro and in vivo (subcutaneous implantations in rabbits). When the premixed CPCs aged in PBS or FBS, results show weight loss of the specimens, decreased pH value and increased calcium ion concentration of aging media. Meanwhile, the setting products convert from dicalcium phosphate dihydrate (DCPD) to dicalcium phosphate anhydrous (DCPA), and no hydroxyapatite deposit. The specimen size and the molecular weight of non-aqueous solvent can modulate the setting product of premixed CPCs. For the larger specimens, DCPA is the main setting product, for the smaller ones, the composite contained DCPD and DCPA. With the decrease of the molecular weight of the non-aqueous solvent PEG, the setting product change from both DCPD and DCPA to DCPA due to the quicker exchange rate of PEG with water. After a period of subcutaneous implantation, the surface of the grafts obviously disintegrated with the formation of porous structures, but their internal morphology do not obviously change.

3.
BMC Complement Altern Med ; 18(1): 296, 2018 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-30404637

RESUMO

BACKGROUND: Oral and pharyngeal cancer is the most common malignant human cancers. Chemotherapy is an effective approach for anti-oral cancer therapy, while the drug tolerance and resistance remain a problem for oral cancer patients. Aloe-emodin, rhein and physcion are classified as anthraquinones, which are the main pharmacodynamic ingredients of Rheum undulatum L.. This study was undertaken to investigate whether aloe-emodin, rhein and physcion show inhibiting growth and inducing apoptosis in oral squamous cell carcinoma SCC15 cells. We found that aloe-emodin show inhibiting growth and inducing apoptosis in oral squamous cell carcinoma SCC15 cells, we also investigated the underlying mechanisms of apoptosis induced by aloe-emodin. METHODS: Thiazolyl blue tetrazolium bromide (MTT) test was used to detect cell proliferation. Cell apoptosis was detected by flow cytometry. We also used western blot analysis to detect the potential mechanisms of apoptosis. RESULTS: Aloe-emodin, rhein and physcion inhibit the proliferation of SCC15 cells and the order of inhibition level are aloe-emodin > Rhein > Physcion, the half maximal inhibitory concentrations (IC50) value of aloe-emodin was 60.90 µM at 48 h of treatment. Aloe-emodin treatment resulted in a time- and dose-dependent decrease in cell viability and increased the apoptotic cell ratio. The results of western blotting showed the expression levels of caspase-9 and caspase-3 proteins increased following aloe-emodin treatment. CONCLUSIONS: Our results revealed that aloe-emodin treatment could inhibit cell viability of SCC15 cells and the potential mechanism of inhibition might be through the induction of apoptosis by regulation of the expression levels of caspase-9 and caspase-3. This indicates that aloe-emodin may be a good agent for anti-oral cancer drug exploring.


Assuntos
Antraquinonas/farmacologia , Apoptose/efeitos dos fármacos , Carcinoma de Células Escamosas/metabolismo , Neoplasias Bucais/metabolismo , Antraquinonas/química , Caspases/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Humanos
4.
Cell Physiol Biochem ; 42(5): 2105-2117, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28810236

RESUMO

BACKGROUND: MicroRNAs (miRNAs) have emerged as key players in various human biological processes, including tumorigenesis. Here, we investigated the roles of miR-375 in the pathogenesis of oral squamous cell carcinoma (OSCC). METHODS: We performed quantitative real-time PCR (qRT-PCR) to detect miR-375 expression in OSCC tissues and corresponding normal oral epithelial tissues and analyze the correlation of miR-375 expression with OSCC metastasis and patient's survival. Then, the effects of miR-375 expression on proliferation, cell cycle, apoptosis and radiosensitivity in OSCC cells were determined by using MTT, flow cytometry and clonogenic survival assays. A dual-luciferase reporter assay was performed to test whether miR-375 binds to the 3'-untranslated region (3'-UTR) of target mRNA. RESULTS: The expression level of miR-375 in OSCC tissues was significantly lower than that in normal oral epithelial tissues, and low miR-375 expression was correlated with higher incidence of lymph node metastasis and poor survival of OSCC patients. Upregulation of miR-375 significantly inhibits growth, induces cell cycle arrest in G0/G1 phase, increases apoptosis and enhances radiosensitivity in OSCC cells. Analysis of luciferase activity demonstrated that miR-375 binds to the 3'-UTR of insulin like growth factor 1 receptor (IGF-1R). Small interfering RNA (shRNA)-mediated IGF-1R knockdown mimics the effects of miR-375 upregulation, while overexpression of IGF-1R partially reverses those effects in OSCC cells. CONCLUSION: It was obviously demonstrated that miRNA-375 inhibits growth and enhances radiosensitivity in OSCC cells by targeting IGF-1R, suggesting that miR-375 may be a potential therapeutic target for OSCC patients.


Assuntos
Carcinoma de Células Escamosas/patologia , MicroRNAs/metabolismo , Neoplasias Bucais/patologia , Receptor IGF Tipo 1/metabolismo , Antagomirs/metabolismo , Apoptose/efeitos da radiação , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/mortalidade , Pontos de Checagem do Ciclo Celular/efeitos da radiação , Linhagem Celular Tumoral , Proliferação de Células , Progressão da Doença , Regulação para Baixo , Raios gama , Humanos , Metástase Linfática , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Neoplasias Bucais/genética , Neoplasias Bucais/mortalidade , Prognóstico , Interferência de RNA , Tolerância a Radiação/efeitos da radiação , Receptor IGF Tipo 1/antagonistas & inibidores , Receptor IGF Tipo 1/genética , Taxa de Sobrevida , Regulação para Cima
5.
Arch Virol ; 162(11): 3549-3550, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28730521

RESUMO

Here, we present the complete genomic sequence of an attenuated duck enteritis virus (DEV). The Chinese standard challenge strain of DEV (DEV CSC) was serially passaged 20 times in chick embryo fibroblasts and then 85 times in chick embryos. The virus was attenuated and was avirulent to 2-month-old ducks. The attenuated DEV genome is 162,131 base pairs (bp) in length and as long as the parental genomic sequence. There are only 22 nucleotide substitutions, resulting in single amino acid changes in open reading frames LORF5, LORF4, UL41, UL39, UL32, UL13, UL10, UL3, US3, US4 and US7. The genome sequence has been deposited in the GenBank database under accession number KU216226. This study provides genetic information about DEV attenuation and further advances our understanding of the molecular basis of DEV pathogenesis.


Assuntos
Patos/virologia , Genoma Viral , Mardivirus/fisiologia , Animais , Células Cultivadas , Embrião de Galinha , DNA Viral/genética , Enterite/veterinária , Enterite/virologia , Fibroblastos/fisiologia , Mardivirus/patogenicidade , Doenças das Aves Domésticas/virologia , Virulência
6.
Anticancer Agents Med Chem ; 17(9): 1234-1242, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-26478521

RESUMO

BACKGROUND: 15,16-dihydrotanshinone I (DHTI), a lipophilic tanshinone extracted from Danshen root (Salvia miltiorrhiza Bunge), has been reported to function as an antitumor agent. However, its activity on osteosarcoma (OS), the most common primary malignant bone tumor, is unclear. OBJECTIVE: This study aimed to determine the effects of DHTI treatment on proliferation, apoptosis and migration of human OS cell line 143B and investigate the possible underlying molecular mechanisms. METHOD: Human cell line 143B was used as a model for investigation of the inhibitory effects of DHTI on osteosarcoma. Cell proliferation was evaluated by MTT assays, while cell cycle progression, apoptosis and cell migration were analyzed by flow cytometer, caspase activity assays and scratch migration assays. qRT-PCR and western blot were carried out to detect the expression levels of representative genes and proteins during physiological processes examined above. RESULTS: DHTI treatment inhibited the proliferation of 143B cells in a dose- and time-dependent manner through arresting cells in G1 phase by reducing the expression of cyclin D1, cyclin E1, CDK2, CDK4, CDK6, p-Rb, E2F1, SKP2 and increasing the expression of P53, P21cip1, P27kip1. In addition, DHTI induced apoptosis of 143B cells through caspase pathways to activate caspase-3, caspase-8, caspase-9, Bax, and PARP cleavage but reduce the expression of Bcl-2. Furthermore, DHTI treatment attenuated cell migration by down-regulating adhesion molecules VCAM-1 and ICAM-1. CONCLUSION: These findings suggest that DHTI could be a novel and efficient therapeutic candidate for OS treatment and further detailed investigation is warranted.


Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Neoplasias Ósseas/tratamento farmacológico , Osteossarcoma/tratamento farmacológico , Fenantrenos/farmacologia , Antineoplásicos/química , Neoplasias Ósseas/patologia , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Osteossarcoma/patologia , Fenantrenos/química , Relação Estrutura-Atividade , Células Tumorais Cultivadas
7.
Arch Virol ; 162(1): 171-179, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27709401

RESUMO

H9 subtype avian influenza viruses (AIVs) remain a significant burden in the poultry industry and are considered to be one of the most likely causes of any new influenza pandemic in humans. As ducks play an important role in the maintenance of H9 viruses in nature, successful control of the spread of H9 AIVs in ducks will have significant beneficial effects on public health. Duck enteritis virus (DEV) may be a promising candidate viral vector for aquatic poultry vaccination. In this study, we constructed a recombinant DEV, rDEV-∆UL2-HA, inserting the hemagglutinin (HA) gene from duck-origin H9N2 AIV into the UL2 gene by homologous recombination. One-step growth analyses showed that the HA gene insertion had no effect on viral replication and suggested that the UL2 gene was nonessential for virus growth in vitro. In vivo tests further showed that the insertion of the HA gene in place of the UL2 gene did not affect the immunogenicity of the virus. Moreover, a single dose of 103 TCID50 of rDEV-∆UL2-HA induced solid protection against lethal DEV challenge and completely prevented H9N2 AIV viral shedding. To our knowledge, this is the first report of a DEV-vectored vaccine providing robust protection against both DEV and H9N2 AIV virus infections in ducks.


Assuntos
Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia , Vírus da Influenza A Subtipo H9N2/imunologia , Vacinas contra Influenza/imunologia , Influenza Aviária/prevenção & controle , Animais , Portadores de Fármacos , Patos , Vetores Genéticos , Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética , Herpesviridae/genética , Vírus da Influenza A Subtipo H9N2/genética , Vacinas contra Influenza/administração & dosagem , Vacinas contra Influenza/genética , Influenza Aviária/virologia , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia
8.
Parasit Vectors ; 9: 498, 2016 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-27623998

RESUMO

BACKGROUND: Tick-borne rickettsioses are considered important emerging zoonoses worldwide, but their etiological agents, rickettsiae, remain poorly characterized in northeastern China, where many human cases have been reported during the past several years. Here, we determined the characteristics of Rickettsia spp. infections in ticks in this area. METHODS: Ticks were collected by flagging vegetation from Jilin and Heilongjiang provinces of northeastern China followed by morphological identification. The presence of Rickettsia spp. in ticks was detected by PCR targeting the 23S-5S ribosomal RNA intergenic spacer, citrate synthase (gltA) gene, and 190-kDa outer membrane protein gene (ompA). The newly-generated sequences were subjected to phylogenetic analysis using the software MEGA 6.0. RESULTS: The overall infection rate of Rickettsia spp. was 6.12 %. Phylogenetic analyses based on the partial gltA and ompA genes demonstrated that rickettsiae detected in the ticks belong to four species, including "Candidatus Rickettsia tarasevichiae", Rickettsia heilongjiangensis, Rickettsia raoultii, and a potential new species isolate. The associated tick species were also identified, i.e. Dermacentor nuttalli and Dermacentor silvarum for R. raoultii, Haemaphysalis concinna and Haemaphysalis longicornis for R. heilongjiangensis, and Ixodes persulcatus for "Ca. R. tarasevichiae". All Rickettsia spp. showed significantly high infection rates in ticks from Heilongjiang when compared to Jilin Province. CONCLUSION: Rickettsia heilongjiangensis, R. raoultii and "Ca. R. tarasevichiae" are widely present in the associated ticks in northeastern China, but more prevalent in Heilongjiang Province. The data of this study increase the information on the distribution of Rickettsia spp. in northeastern China, which have important public health implications in consideration of their recent association with human diseases.


Assuntos
Rickettsia/genética , Rickettsia/isolamento & purificação , Carrapatos/microbiologia , Animais , China , Ninfa/microbiologia , Filogenia
9.
J Nanosci Nanotechnol ; 16(3): 2277-82, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27455629

RESUMO

Calcium sulfate dihydrate (CaSO4 x 2H2O, CSD) was widely used as the artificial bone graft. In this study, two kinds of CSD materials were characterized with XRD, TG/DTA, FT-IR, and SEM. They were both composed of CSD. Spherical shape particles were observed for nano-CSD with diameters of 52-300 nm. The micro-CSD were thin sheet particles with dimensions of 5-10 µm. At 56 days post-implantation in vivo, nano-CSD had good tissue compatibility. A frequently used bioactive material DBM, which was the combination of nano-CSD (nano-CSD-DBM) and micro-CSD (micro-CSD-DBM) in a 1:1 weight ratio separately. Composite materials were implanted in intramuscular pockets in nude mouse model. New bone mineralization could be both observed in the surgery site. Collagen I was also widely distributed by immunohistochemistry assay. And new bone area of nano-CSD-DBM was 28 ± 4.6% at 4 weeks post-operation. But new bone area of micro-CSD-DBM was 16 ± 3.7% (less than nano-CSD-DBM). Nano-CSD showed increased degradation rate with obvious anginogenicity. And nano-CSD-DBM showed more excellent bone induction property as bone substitute implant.


Assuntos
Sulfato de Cálcio/química , Nanoestruturas , Osteogênese , Animais , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Microscopia Eletrônica de Varredura , Espectroscopia de Infravermelho com Transformada de Fourier , Termogravimetria , Difração de Raios X
10.
J Nanosci Nanotechnol ; 16(3): 2384-9, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27455645

RESUMO

Spherical nanocrystal of apatite has been proved to be beneficial for osteoblast growth. Two apatites with spherical nanocrystal morphology were prepared in this study by chemical wet method and further sintering process. SEM exhibited that both apatites had spherical nanocrystal morphology. The crystal morphology and size was approaching to each other. XRD showed the apatites separately were hydroxyapatite and tricalcium phosphate phases. The cellular biocompatibility was evaluated by osteoblasts for these two spherical nanocrstal apatites. The MTT result indicated a higher cell proliferation rate for spherical tricalcium phosphate group. The ALP activity assay also strongly favored the tricalcium phosphate group. RT-PCR results indicated that Collagen I had a higher transcription level on the spherical tricalcium phosphate group. SEM results showed robust cell growth on the materials. It was concluded that the spherical nanophase tricalcium phosphate was superior to the cellular biocompatibility of spherical nanophase hydroxyapatite and the results were helpful in the manufacture of more suitable tissue engineering scaffolds.


Assuntos
Apatitas/química , Nanopartículas , Células 3T3 , Fosfatase Alcalina/metabolismo , Animais , Camundongos , Microscopia Eletrônica de Varredura , Estrutura Molecular , Difração de Pó , Reação em Cadeia da Polimerase em Tempo Real
11.
J Phys Chem A ; 120(26): 4505-13, 2016 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-27300636

RESUMO

N-nitrosodimethylamine (NDMA), as a representative of endogenously formed N-nitroso compounds (NOCs), has become the focus of considerable research interest due to its unusually high carcinogenicity. In this study, effects of ethanol and acetic acid on the formation of NDMA from dimethylamine (DMA) and nitrite in simulated gastric fluid (SGF) were investigated. Experimental results showed that ethanol in the concentrations of 1-8% (v/v) and acetic acid in the concentrations of 0.01-8% (v/v) exhibit inhibitory and promotion effects on the formation of NDMA, respectively. Moreover, they are both in a dose-dependent manner with the largest inhibition/promotion rate reaching ∼70%. Further experimental investigations indicate that ethanol and acetic acid are both able to scavenge nitrite in SGF. It implies that there are interactions of ethanol and acetic acid with nitrite or nitrite-related nitrosating agents rather than DMA. Theoretical calculations confirm the above experimental results and demonstrate that ethanol and acetic acid can both react with nitrite-related nitrosating agents to produce ethyl nitrite (EtONO) and acetyl nitrite (AcONO), respectively. Furthermore, the reactivities of ethyl nitrite, acetyl nitrite, and dinitrogen trioxide reacting with DMA were found in the order of AcONO > N2O3 ≫ EtONO. This is probably the main reason why there are completely different effects of ethanol and acetic acid on NDMA formation. On the basis of the above results, two requirements for a potential inhibitor of NOCs formation in SGF were provided. The results obtained in this study will be helpful in better understanding the inhibition/promotion mechanisms of compounds on NDMA formation in SGF and searching for protective substances to prevent carcinogenic NOCs formation.


Assuntos
Ácido Acético/química , Materiais Biomiméticos/química , Líquidos Corporais/metabolismo , Carcinógenos/química , Dimetilnitrosamina/química , Etanol/química , Estômago , Carcinógenos/metabolismo , Dimetilnitrosamina/metabolismo , Modelos Moleculares , Conformação Molecular
12.
Biomed Res Int ; 2016: 8701423, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27294141

RESUMO

Background. The purpose of this study is to understand the oral mucosal immune status of cancer patients and to make clear whether antibacterial proteins such as salivary secretory immunoglobulin (SIgA) and lysozyme in saliva were influenced by patients' health status and certain medical treatment therapy. Materials and Methods. This study included 221 patients with malignant tumor receiving antineoplastic treatment and 171 age- and gender-matched healthy controls. Results. The results showed that patients suffering malignant tumor had lower level of SIgA and higher level of lysozyme than healthy subjects (P < 0.05). The SIgA level was significantly different among different cancer tumors, while the lysozyme level showed significant difference only between patients with digestive tract malignant tumor and hematopoietic system tumor. Pretreatment before transplantation for hematopoietic system tumor patients significantly affected the lysozyme level other than SIgA. SIgA level was affected by many factors such as age, therapy factors, and oral hygiene. Conclusion. Malignant tumor and the antineoplaston may weaken the patients' oral mucosal immunity, influence levels of some salivary proteins, and decrease the level of SIgA, resulting in aggregation of oral bacteria and failure of clearing them from the oral cavity.


Assuntos
Imunoglobulina A Secretora/metabolismo , Muramidase/metabolismo , Neoplasias/metabolismo , Saliva/metabolismo , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/enzimologia
13.
Artigo em Inglês | MEDLINE | ID: mdl-27235999

RESUMO

Color is an important property for food evaluation. Synthetic azo dyes are usually used in food product to obtain better appearance because of their stability and low cost. However, such dyes should be strictly controlled because of their potential threat to human health. A simple, rapid and sensitive method has been developed to determine orange II, allura red, and para red simultaneously by ion mobility spectrometry. The three dyes could be separated at the same time and the migration time of orange II, allura red, and para red are 12.070±0.010, 8.180±0.015, and 11.037±0.016ms, respectively. The effects of different parameters, such as pH, solvent, percentage of water, were investigated to establish the optimal condition. The detection limits were 0.1, 0.05, and 0.2µg/mL for orange II, allura red, and para red, respectively. The recoveries of the three azo dyes from jellies were all higher than 81%. The developed method is fast and accurate for the detection of the three synthetic dyes.


Assuntos
Compostos Azo/análise , Análise de Alimentos/métodos , Compostos Azo/química , Concentração de Íons de Hidrogênio , Limite de Detecção , Espectrometria de Massas
14.
Matrix Biol ; 52-54: 426-441, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26780725

RESUMO

For more than 100years, cells and tissues have been studied in vitro using glass and plastic surfaces. Over the last 10-20years, a great body of research has shown that cells are acutely sensitive to their local environment (extracellular matrix, ECM) which contains both chemical and physical cues that influence cell behavior. These observations suggest that modern cell culture systems, using tissue culture polystyrene (TCP) surfaces, may fail to reproduce authentic cell behavior in vitro, resulting in "artificial outcomes." In the current study, we use bone marrow (BM)- and adipose (AD)-derived stromal cells to prepare BM-ECM and AD-ECM, which are decellularized after synthesis by the cells, to mimic the cellular niche for each of these tissues. Each ECM was characterized for its ability to affect BM- and AD-mesenchymal stem cell (MSC) proliferation, as well as proliferation of three cancer cell lines (HeLa, MCF-7, and MDA-MB-231), modulate cell spreading, and direct differentiation relative to standard TCP surfaces. We found that both ECMs promoted the proliferation of MSCs, but that this effect was enhanced when the tissue-origin of the cells matched that of the ECM (i.e. BM-ECM promoted the proliferation of BM-MSCs over AD-MSCs, and vice versa). Moreover, BM- and AD-ECM were shown to preferentially direct MSC differentiation towards either osteogenic or adipogenic lineage, respectively, suggesting that the effects of the ECM were tissue-specific. Further, each ECM influenced cell morphology (i.e. circularity), irrespective of the origin of the MSCs, lending more support to the idea that effects were tissue specific. Interestingly, unlike MSCs, these ECMs did not promote the proliferation of the cancer cells. In an effort to further understand how these three culture substrates influence cell behavior, we evaluated the chemical (protein composition) and physical properties (architecture and mechanical) of the two ECMs. While many structural proteins (e.g. collagen and fibronectin) were found at equivalent levels in both BM- and AD-ECM, the architecture (i.e. fiber orientation; surface roughness) and physical properties (storage modulus, surface energy) of each were unique. These results, demonstrating differences in cell behavior when cultured on the three different substrates (BM- and AD-ECM and TCP) with differences in chemical and physical properties, provide evidence that the two ECMs may recapitulate specific elements of the native stem cell niche for bone marrow and adipose tissues. More broadly, it could be argued that ECMs, elaborated by cells ex vivo, serve as an ideal starting point for developing tissue-specific culture environments. In contrast to TCP, which relies on the "one size fits all" paradigm, native tissue-specific ECM may be a more rational model to approach engineering 3D tissue-specific culture systems to replicate the in vivo niche. We suggest that this approach will provide more meaningful information for basic research studies of cell behavior as well as cell-based therapeutics.


Assuntos
Tecido Adiposo/citologia , Células da Medula Óssea/citologia , Técnicas de Cultura de Células/métodos , Matriz Extracelular/metabolismo , Células-Tronco Mesenquimais/citologia , Tecido Adiposo/metabolismo , Células da Medula Óssea/metabolismo , Diferenciação Celular , Linhagem Celular Tumoral , Proliferação de Células , Células HeLa , Humanos , Células MCF-7 , Células-Tronco Mesenquimais/metabolismo , Poliestirenos/química , Nicho de Células-Tronco
15.
Clin Oral Investig ; 20(3): 459-67, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26220512

RESUMO

OBJECTIVES: Oral colonization of Candida could lead to later development of oropharyngeal candidiasis or candidemia among the immunocompromised patients. This study aims to describe the occurrence and risk factors of oral Candida colonization in patients with malignancies. MATERIALS AND METHODS: From October 2012 to March 2013, 78 patients with pulmonary cancer (group I), 101 patients with gastrointestinal tract tumor (group II), 79 patients with hematopoietic system malignant tumor (group III), and 101 healthy controls were consecutively recruited in a hospital in Beijing, China. The oral rinse samples were taken and Candida species were identified; the enzymes activities were tested. RESULTS: In total, 110 and 27 Candida strains were isolated from 91 patients and 26 controls, respectively. The oral colonization rate with Candida albicans in group III (12.7 %) was significant lower than that in group I (30.8 %), group II (33.7 %), and control group (25.7 %). The oral colonization rates with non-albicans Candida species in group I, group II, and group III were 15.4, 10.9, and 12.7 %, respectively, while only one non-albicans Candida strain was identified in control group. The non-albicans Candida species exhibited a lower virulence than C. albicans. Age was an independent risk factor for Candida colonization in patients with pulmonary cancer and digestive tract malignant tumor, "Teeth brush <1 time/day" was an independent risk factor for Candida colonization in patients with hematopoietic system tumor. CONCLUSIONS: The differences of risk factors for oral Candida colonization in patients with different cancers require different strategies for the prevention and control of Candida infection. CLINICAL RELEVANCE: Old aged patients with pulmonary cancer and digestive tract malignant tumor are high-risk population for Candida colonization. Increasing frequency of teeth brush might be helpful for preventing Candida colonization.


Assuntos
Candidíase Bucal/epidemiologia , Candidíase Bucal/microbiologia , Neoplasias Gastrointestinais/complicações , Neoplasias Gastrointestinais/imunologia , Neoplasias Hematológicas/complicações , Neoplasias Hematológicas/imunologia , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares/imunologia , Infecções Oportunistas/epidemiologia , Adulto , Antifúngicos/farmacologia , Estudos de Casos e Controles , China/epidemiologia , Feminino , Genótipo , Humanos , Hospedeiro Imunocomprometido , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Estudos Prospectivos , Fatores de Risco , Escovação Dentária , Virulência
16.
J Nanosci Nanotechnol ; 15(6): 4641-7, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26369091

RESUMO

This study aims to assess the effects of diamond-like carbon (DLC) films on fretting wear behavior of orthodontic archwire-bracket contacts. 'Mirror-confinement-type electron cyclotron resonance (MCECR) plasma sputtering' was utilized to deposit carbon films on stainless steel archwires and brackets. Nanostructure of carbon films such as the bonding structure, cross-sectional thickness and surface roughness were studied. The fretting wear behavior of various archwire-bracket contacts were investigated by using a self-developed tester in ambient air and artificial saliva. The results indicated that DLC-coated wires showed significantly low friction coefficient than the uncoated wires independently of the applied environments. Nevertheless, the DLC-coated and uncoated brackets showed no significant differences in the friction coefficient. Microscopic analysis showed that low wear took place for the DLC-coated surfaces. It is proposed that the application of DLC coating on archwires can decrease the orthodontic fretting wear and coefficient of friction. Unfortunately it does not affect the frictional properties for brackets at present.

17.
Sci Rep ; 5: 12593, 2015 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-26219508

RESUMO

The present study tested whether activation of the sympathetic tone by aberrant joint loading elicits abnormal subchondral bone remodeling in temporomandibular joint (TMJ) osteoarthritis. Abnormal dental occlusion was created in experimental rats, which were then intraperitoneally injected by saline, propranolol or isoproterenol. The norepinephrine contents, distribution of sympathetic nerve fibers, expression of ß-adrenergic receptors (ß-ARs) and remodeling parameters in the condylar subchondral bone were investigated. Mesenchymal stem cells (MSCs) from condylar subchondral bones were harvested for comparison of their ß-ARs, pro-osteoclastic gene expressions and pro-osteoclastic function. Increases in norepinephrine level, sympathetic nerve fiber distribution and ß2-AR expression were observed in the condylar subchondral bone of experimental rats, together with subchondral bone loss and increased osteoclast activity. ß-antagonist (propranolol) suppressed subchondral bone loss and osteoclast hyperfunction while ß-agonist (isoproterenol) exacerbated those responses. MSCs from experimental condylar subchondral bone expressed higher levels of ß2-AR and RANKL; norepinephrine stimulation further increased their RANKL expression and pro-osteoclastic function. These effects were blocked by inhibition of ß2-AR or the PKA pathway. RANKL expression by MSCs decreased after propranolol administration and increased after isoproterenol administration. It is concluded that ß2-AR signal-mediated subchondral bone loss in TMJ osteoarthritisis associated with increased RANKL secretion by MSCs.


Assuntos
Reabsorção Óssea/metabolismo , Osso e Ossos/metabolismo , Osteoartrite/metabolismo , Receptores Adrenérgicos beta/metabolismo , Transdução de Sinais/fisiologia , Articulação Temporomandibular/metabolismo , Adrenérgicos/farmacologia , Animais , Remodelação Óssea/efeitos dos fármacos , Remodelação Óssea/fisiologia , Reabsorção Óssea/patologia , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/patologia , Feminino , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Células-Tronco Mesenquimais/patologia , Norepinefrina/farmacologia , Osteoartrite/patologia , Osteoclastos/efeitos dos fármacos , Osteoclastos/metabolismo , Osteoclastos/patologia , Ligante RANK/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Articulação Temporomandibular/efeitos dos fármacos , Articulação Temporomandibular/patologia
18.
Cell Tissue Res ; 362(2): 399-405, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26149648

RESUMO

We investigate whether the expression of the receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) in human dental follicle cells (HDFCs) regulated by colony stimulating factor 1 (CSF-1), parathyroid hormone-related protein (PTHrP) and bone morphogenetic protein-2 (BMP-2) contributes to osteoclastogenesis. Adolescent human impacted third mandibular molars were used to separate HDFCs. These cells were incubated with PTHrP (10 ng/ml), CSF-1 (25 ng/ml), or BMP-2 (100 ng/ml) for 0.5, 1, 3, 6 and 12 h. The expression of OPG and RANKL was investigated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Two co-culture systems and tartrate-resistant acid phosphatase (TRAP) staining were used to examine osteoclast formation. Scanning electron microscopy was utilized for the resorption pit assay. RANKL and OPG were expressed innately in HDFCs. Exogenous PTHrP, CSF-1 and BMP-2 chronologically regulated the expression of RANKL and OPG in HDFCs. PTHrP and CSF-1 had similar regulative patterns leading to the up-regulated expression of RANKL and the down-regulated expression of OPG and opposite for BMP-2. The number of TRAP-positive peripheral blood mononuclear cells (PBMCs) slightly increased in contacted co-culture of HDFCs and PBMCs, whereas secreted OPG from HDFCs inhibited osteoclastogenesis in the transwell co-culture system. Contacted co-culture of HDFCs and PBMCs exhibited small and shallow resorption pits, whereas in the transwell co-culture system, secreted OPG from HDFCs reduced the resorption pits, reflecting the difference in osteoclast production. Collectively, we found a dual action of HDFCs in osteoclastogenesis; moreover, PTHrP, CSF-1 and BMP-2 might influence osteoclastogenesis by regulating the expression of RANKL and OPG in HDFCs.


Assuntos
Saco Dentário/metabolismo , Leucócitos Mononucleares/metabolismo , Glicoproteínas de Membrana/metabolismo , Osteoclastos/metabolismo , Osteoprotegerina/metabolismo , Receptor Ativador de Fator Nuclear kappa-B/metabolismo , Adolescente , Células Cultivadas , Técnicas de Cocultura/métodos , Humanos
19.
Cell Prolif ; 48(4): 436-42, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26032517

RESUMO

OBJECTIVES: MOF (males absent on the first) is a histone acetyltransferase belonging to the MYST (MOZ, Ybf2/Sas3, Sas2 and TIP60) family. In mammals, MOF plays critical roles in transcription activation by acetylating histone H4 at K16. Human MOF (hMOF) essentially participates in behaviour of several human cancers. However, its role in human oral tongue squamous cell carcinoma (OTSCC) remains elusive, but we propose that hMOF regulates OTSCC cell population growth. MATERIALS AND METHODS: Real time PCR and western blot analysis were applied, and it was found that hMOF level was up-regulated in human OTSCC. High hMOF expression predicted poor overall and disease-free survival. hMOF knockdown attenuated OTSCC cell growth and transformation. RESULTS: EZH2 (enhancer of zeste homolog 2) was up-regulated in human OTSCC tissues and its level positively correlated with level of hMOF. hMOF knockdown inhibited EZH2 expression by reducing its promoter activity. Moreover, we have demonstrated that EZH2 was critically essential for function of hMOF in human OTSCC. CONCLUSIONS: Human males absent on the first regulated OSTCC growth through EZH2, thus EZH2 may serve as a candidate for anti-OTSCC therapy.


Assuntos
Carcinoma de Células Escamosas/genética , Regulação Neoplásica da Expressão Gênica , Histona Acetiltransferases/genética , Complexo Repressor Polycomb 2/genética , Neoplasias da Língua/genética , Animais , Carcinoma de Células Escamosas/patologia , Proteína Potenciadora do Homólogo 2 de Zeste , Feminino , Humanos , Masculino , Camundongos Endogâmicos BALB C , Língua/metabolismo , Língua/patologia , Neoplasias da Língua/patologia
20.
J Oral Maxillofac Surg ; 73(10): 1970-6, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25930960

RESUMO

Condyle fractures are common in children and are increasingly treated with open reduction. Three-dimensional printing has developed into an important method of assisting surgical treatment. This report describes the case of a 14-year-old patient treated for a right condyle fracture at the authors' hospital. Preoperatively, the authors designed a surgical guide using 3-dimensional printing and virtual surgery. The 3-dimensional surgical guide allowed accurate alignment of the fracture using Kirschner wire without additional dissection and tissue injury. Kirschner wire fixation augmented by 3-dimensional printing technology produced a good outcome in this adolescent condyle fracture.


Assuntos
Fios Ortopédicos , Côndilo Mandibular/lesões , Fraturas Mandibulares/cirurgia , Impressão Tridimensional , Adolescente , Feminino , Humanos
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