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1.
Adv Mater ; : e1907288, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31977113

RESUMO

In a modern electronics system, charge-coupled devices and data storage devices are the two most indispensable components. Although there has been rapid and independent progress in their development during the last three decades, a cofunctionality of both sensing and memory at single-unit level is yet premature for flexible electronics. For wearable electronics that work in ultralow power conditions and involve strains, conventional sensing-and-memory systems suffer from low sensitivity and are not able to directly transform sensed information into sufficient memory. Here, a new transformative device is demonstrated, which is called "sen-memory", that exhibits the dual functionality of sensing and memory in a monolithic integrated circuit. The active channel of the device is formed by a carbon nanotube thin film and the floating gate is formed by a controllably oxidized aluminum nanoparticle array for electrical- and optical-programming. The device exhibits a high on-off current ratio of ≈106 , a long-term retention of ≈108 s, and durable flexibility at a bending strain of 0.4%. It is shown that the device senses a photogenerated pattern in seconds at zero bias and memorizes an image for a couple of years.

2.
J Asian Nat Prod Res ; : 1-8, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-31163996

RESUMO

Zeaxanthin dipalmitate (3) and two zeaxanthin dipalmitate derivatives, including one new compound (1), were obtained from wolfberry [the fruit of Lycium barbarum L. (Solanaceae)]. Their structures were unambiguously elucidated by spectroscopic analyses. Compound 2 is isolated from the genus Lycium for the first time, and its 1D/2D NMR data are firstly reported. All the compounds belong to carotenoids which are a kind of major bioactive constituents in wolfberry and are also responsible for wolfberry's red color.

3.
Phytochemistry ; 164: 60-66, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31096077

RESUMO

Fifteen phenylpropanoid glycosides, including six undescribed compounds were isolated from the fruit of Lycium barbarum L. (Solanaceae) (goji or wolfberry). Their structures were identified by detailed spectroscopic analyses. Seven known compounds were firstly isolated from the genus Lycium, in which the 1D and 2D NMR data of one compound were reported for the first time. Notably, two undescribed compounds were a pair of rare tautomeric glycoside anomers characterized by the presence of free anomeric hydroxy. Antioxidant and hypoglycemic activities of all these compounds were assessed using DPPH radical scavenging, oxygen radical absorbance capacity (ORAC), and α-glucosidase inhibitory assays, respectively. These compounds showed different levels of oxygen radical absorbance capacity, and some isolates exhibited potent antioxidant activity with greater ORAC values than the positive control (EGCG).


Assuntos
Antioxidantes/farmacologia , Frutas/química , Inibidores de Glicosídeo Hidrolases/farmacologia , Glicosídeos/farmacologia , Lycium/química , Fenilpropionatos/farmacologia , Antioxidantes/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/antagonistas & inibidores , Relação Dose-Resposta a Droga , Inibidores de Glicosídeo Hidrolases/química , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Glicosídeos/química , Glicosídeos/isolamento & purificação , Conformação Molecular , Fenilpropionatos/química , Fenilpropionatos/isolamento & purificação , Picratos/antagonistas & inibidores , Espécies Reativas de Oxigênio/antagonistas & inibidores , Relação Estrutura-Atividade , alfa-Glucosidases/metabolismo
4.
ACS Appl Mater Interfaces ; 11(12): 11699-11705, 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30839190

RESUMO

Carbon nanotube (CNT) thin-film transistors are expected to be promising for use in flexible electronics including flexible and transparent integrated circuits and in wearable chemical and physical sensors and for driving the circuits of flexible display panels. However, current devices based on CNT channels suffer from poor performance uniformity and low manufacturing yield; therefore, they are still far from being practical. This is usually caused by nonuniform deposition of the semiconducting CNTs and the rough surface of flexible substrates. Here, we report CNT thin-film transistors (TFTs) driving a flexible 64 × 64 pixel active matrix light-emitting diode display (AMOLED) by improving the formation of uniform CNT films and developing a new pretreatment technique for flexible substrates. The achieved AMOLED has uniform brightness and a high yield of 99.93% in its 4096 pixels. More than 8000 TFTs with high-purity semiconducting CNTs as the channel material show an average on-off current ratio of ∼107 and a carrier mobility of 16 cm2 V-1 s-1. The standard deviations of the on-state current and the carrier mobility are 4.1 and 6.5%, respectively. Our result shows that the panel driven by high-purity semiconducting CNTs is a promising strategy for the development of next-generation flexible, large-area displays.

5.
BMC Urol ; 18(1): 79, 2018 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-30200918

RESUMO

BACKGROUND: BRAF-activated long non-coding RNA (BANCR) has been associated with various types of cancer. Nevertheless, the role of BANCR in clear cell renal cell carcinoma (ccRCC) is still not fully understood. This study aims to investigate the relationship between ccRCC and BANCR. METHODS: Expression of BANCR in TCGA renal cancer data sets was analyzed. The expression pattern of BANCR in Immortalized normal human proximal tubule epithelial cell line HK-2 and ccRCC cell lines (ACHN, CAKI-1, A498 and 786-O) was detected by real-time quantitative RT-PCR (qRT-PCR). ccRCC tissues with adjacent normal renal tissues diagnosed by pathological methods from 62 patients were used to detect the expression of BANCR, and its correlation with prognosis of ccRCC patients was assessed by Kaplan-Meier method. The LV-BANCR vector was used to examine the influence of BANCR on the proliferation, migration, invasion, apoptosis and cell cycle distribution of ccRCC cells in vitro. RESULTS: BANCR was downregulated in renal cancer according to TCGA data sets. Compared with adjacent normal renal tissues and normal human proximal tubule epithelial cell line HK-2, BANCR expression was significantly decreased in ccRCC tissues and ccRCC cell lines, and its low expression was associated with poor prognosis. Moreover, in the condition of BANCR overexpression by LV-BANCR vector, the proliferation, migration, invasion capacity of ccRCC cells was inhibited, while the apoptosis was increased and the G1 cell cycle arrest was induced in vitro. CONCLUSIONS: BANCR is downregulated in ccRCC tissues and cell lines, and is associated with ccRCC progression. Thus, BANCR may represent a novel prognostic biomarker and a potential therapeutic target for ccRCC patients.


Assuntos
Carcinoma de Células Renais/metabolismo , Regulação Neoplásica da Expressão Gênica , Neoplasias Renais/metabolismo , Proteínas Proto-Oncogênicas B-raf/metabolismo , RNA Longo não Codificante/metabolismo , Biomarcadores Tumorais , Carcinoma de Células Renais/genética , Linhagem Celular Tumoral , Proliferação de Células , Regulação para Baixo , Humanos , Estimativa de Kaplan-Meier , Rim/metabolismo , Neoplasias Renais/genética , Prognóstico , Proteínas Proto-Oncogênicas B-raf/genética
6.
Adv Sci (Weinh) ; 5(5): 1700965, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29876218

RESUMO

This study reports a simple and effective technique for the high-throughput fabrication of flexible all-carbon nanotube (CNT) electronics using a photosensitive dry film instead of traditional liquid photoresists. A 10 in. sized photosensitive dry film is laminated onto a flexible substrate by a roll-to-roll technology, and a 5 µm pattern resolution of the resulting CNT films is achieved for the construction of flexible and transparent all-CNT thin-film transistors (TFTs) and integrated circuits. The fabricated TFTs exhibit a desirable electrical performance including an on-off current ratio of more than 105, a carrier mobility of 33 cm2 V-1 s-1, and a small hysteresis. The standard deviations of on-current and mobility are, respectively, 5% and 2% of the average value, demonstrating the excellent reproducibility and uniformity of the devices, which allows constructing a large noise margin inverter circuit with a voltage gain of 30. This study indicates that a photosensitive dry film is very promising for the low-cost, fast, reliable, and scalable fabrication of flexible and transparent CNT-based integrated circuits, and opens up opportunities for future high-throughput CNT-based printed electronics.

7.
Adv Mater ; 30(32): e1802057, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29952030

RESUMO

Single-wall carbon nanotubes (SWCNTs), especially in the form of large-area and high-quality thin films, are a promising material for use in flexible and transparent electronics. Here, a continuous synthesis, deposition, and transfer technique is reported for the fabrication of meter-scale SWCNT thin films, which have an excellent optoelectrical performance including a low sheet resistance of 65 Ω/◽ with a transmittance of 90% at a wavelength of 550 nm. Using these SWCNT thin films, high-performance all-CNT thin-film transistors and integrated circuits are demonstrated, including 101-stage ring oscillators. The results pave the way for the future development of large-scale, flexible, and transparent electronics based on CNT thin films.

8.
J Nanosci Nanotechnol ; 15(4): 3212-7, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26353565

RESUMO

Vertically aligned carbon nanotube (VACNT) array/polymer composite has already been recognized as a promising candidate for advanced thermal pad in thermal management of high-power electronic devices. However, the thermal conductive performance of this composite was limited by the quality of CNTs arrays. In this study, pre-annealing treatment was used to purify CNT arrays and improve thermal conductive performance of VACNT arrays/silicone composite. The thermal conductivity of the composite was enhanced by 34.52% and the thermal interface resistance was also reduced by 65.94% at a pre-annealing temperature of 490 °C for 5 min. The annealing process could remove some amorphous carbon and open the tips of CNTs. As a result, the interfacial compatibility in composite between carbon nanotube and polymer matrix was improved. The cyclic compression and tension performance of VACNT/S160 composite was investigated for further application.

9.
Int J Clin Exp Pathol ; 8(4): 3765-74, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26097558

RESUMO

OBJECTIVE: accumulating evidence suggest that long non-coding RNAs (lncRNAs) may play important roles in human cancers. LncRNA neuroblastoma associated transcript-1 (NBAT-1) was initially identified to be involved in the progression of neuroblastoma. However, there is no report about the role of NBAT-1 in clear cell renal cell carcinoma (ccRCC). The purpose of this study is to investigate the clinical significant of NBAT-1 in ccRCC. METHODS: the expression pattern of NBAT-1 in ccRCC patients and renal cancer cell lines was detected by using quantitative real-time PCR (qRT-PCR), and its correlation with clinicopathologic features and prognosis of patients with ccRCC was assessed by Kaplan-Meier method and Cox proportional hazards model, respectively. Small interfering RNA (siRNA) was transfected into 786-O and ACHN cells to determine the effect of NBAT-1 knockdown on renal cancer cells. RESULT: NBAT-1 expression is significantly decreased in ccRCC tissues and renal cancer cells compared with adjacent normal tissues and normal human proximal tubule epithelial cell line HK-2, and its low level is associated with advanced features and poor prognosis. Also, multivariate analysis identified NBAT-1 expression as an independent prognostic factor for ccRCC. In vitro assays indicated that knockdown of NBAT-1 expression increased renal cancer cell proliferation, migration and invasion. CONCLUSIONS: NBAT-1 is a novel molecular correlated with ccRCC progression; and it may represent a prognostic biomarker and therapeutic target in renal cancer diagnosis and treatment.


Assuntos
Carcinoma de Células Renais/metabolismo , Neoplasias Renais/metabolismo , RNA Longo não Codificante/metabolismo , Idoso , Carcinoma de Células Renais/genética , Carcinoma de Células Renais/mortalidade , Carcinoma de Células Renais/patologia , Linhagem Celular Tumoral , Proliferação de Células , Progressão da Doença , Feminino , Humanos , Neoplasias Renais/genética , Neoplasias Renais/mortalidade , Neoplasias Renais/patologia , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Estadiamento de Neoplasias , Prognóstico , RNA Longo não Codificante/genética , RNA Interferente Pequeno , Taxa de Sobrevida
10.
Med Sci Monit ; 21: 1057-65, 2015 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-25863938

RESUMO

BACKGROUND: A fusion protein composed of heme oxygenase-1 (HO-1) and cell-penetrating peptide PEP-1 has been shown to reduce local intestinal injury after intestinal ischemia/reperfusion (I/R). In this study, we investigated the effects of PEP-1-HO-1 fusion protein on remote organ injury induced by intestinal I/R in rats. MATERIAL AND METHODS: We randomly assigned 24 male Sprague-Dawley rats to 3 groups: Sham, I/R, and I/R plus PEP-1-HO-1 treatment (HO). The model of intestinal I/R was established by occluding the superior mesenteric artery for 45 min followed by 120-min reperfusion. In HO group, PEP-1-HO-1 was administered intravenously 30 min before ischemia, while animals in the Sham and I/R groups received the equal volume of physiological saline. At the end of the experiment, lung, liver, and blood samples were collected and analyzed. RESULTS: Malondialdehyde levels and histological injury scores were increased, and superoxide dismutase activities were decreased in the lung and liver tissues in the I/R group compared with the Sham group (P<0.05). Serum levels of alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor-α, interleukin-6, and lung tissue wet weight to dry weight ratio were increased in the I/R group compared with the Sham group (P<0.05). NF-κB expression in intestinal tissues was significantly higher in the I/R group than in the Sham group. These changes were significantly reversed by treatment with PEP-1-HO-1. CONCLUSIONS: This study demonstrates that administration of PEP-1-HO-1 has a protective role against lung and liver injury after intestinal I/R, attributable to the reduction of released proinflammatory cytokines regulated by NF-κB.


Assuntos
Heme Oxigenase-1/uso terapêutico , Intestinos/irrigação sanguínea , Fígado/patologia , Pulmão/patologia , Proteínas Recombinantes de Fusão/uso terapêutico , Traumatismo por Reperfusão/terapia , Transdução Genética , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Heme Oxigenase-1/genética , Interleucina-6/sangue , Intestinos/patologia , Fígado/enzimologia , Pulmão/enzimologia , Masculino , Malondialdeído/metabolismo , NF-kappa B/metabolismo , Tamanho do Órgão , Ratos Sprague-Dawley , Proteínas Recombinantes de Fusão/genética , Traumatismo por Reperfusão/sangue , Traumatismo por Reperfusão/patologia , Superóxido Dismutase/metabolismo , Fator de Necrose Tumoral alfa/sangue
11.
Sci Rep ; 3: 2263, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23877331

RESUMO

Oxygen conversion process between O2 and H2O by means of electrochemistry or photochemistry has lately received a great deal of attention. Cobalt-phosphate (Co-Pi) catalyst is a new type of cost-effective artificial oxygen-evolving complex (OEC) with amorphous features during photosynthesis. However, can such Co-Pi OEC also act as oxygen reduction reaction (ORR) catalyst in electrochemical processes? The question remains unanswered. Here for the first time we demonstrate that Co-Pi OEC does be rather active for the ORR. Particularly, Co-Pi OEC anchoring on reduced graphite oxide (rGO) nanosheet is shown to possess dramatically improved electrocatalytic activities. Differing from the generally accepted role of rGO as an "electron reservoir", we suggest that rGO serves as "peroxide cleaner" in enhancing the electrocatalytic behaviors. The present study may bridge the gap between photochemistry and electrochemistry towards oxygen conversion.


Assuntos
Cobalto/química , Grafite/química , Nanoestruturas/química , Óxidos/química , Oxigênio/química , Fosfatos/química , Catálise , Eletroquímica , Nanoestruturas/ultraestrutura , Fotoquímica , Espectroscopia Fotoeletrônica , Água/química
12.
Am J Chin Med ; 41(3): 665-82, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23711148

RESUMO

Norcantharidin (NCTD) is currently used for anticancer therapy but the exact mechanism of action remains unknown. Pre-replicative complexes (pre-RCs) are essential for cell DNA replication and highly related to malignant proliferation. Here, we examined the inhibitory effect of NCTD on pre-RC components in HepG2 cells. We showed that NCTD induced degradation of Cdc6 and Mcm2 in a dose-dependent manner. Under 100 µM NCTD concentration, about 70% of Cdc6 and 50% of Mcm2 were degraded. In addition, the nuclear translocation of Mcm6 was inhibited by NCTD. Further studies aiming at G1 synchronous cells showed that, NCTD reduced the chromatin-bound Cdc6, Mcm2 and Mcm6. Moreover, the cells were blocked from entering the S phase and accumulated at the G1 phase when released synchronously into the cell cycle. Consistently, the DNA replication was inhibited by NCTD. Finally, the combination NCTD with Cdc6 depletion lead to more severe cytotoxicity (88%) than NCTD (52%) and Cdc6 depletion (39%) alone. A synergic cytotoxicity was observed between Cdc6 depletion and NCTD. In conclusion, our results demonstrate that NCTD inhibits pre-RC assembly; subsequently blocks the G1 to S transition; and inhibits DNA replication in HepG2 cells. Pre-RCs are an intriguing target for cancer therapy, which merits further investigations for anticancer development.


Assuntos
Antineoplásicos/uso terapêutico , Compostos Bicíclicos Heterocíclicos com Pontes/uso terapêutico , Proteínas de Ciclo Celular/metabolismo , Ciclo Celular/efeitos dos fármacos , Replicação do DNA/efeitos dos fármacos , Neoplasias Hepáticas/tratamento farmacológico , Proteínas Nucleares/metabolismo , Antineoplásicos/farmacologia , Transporte Biológico , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Cantaridina/análogos & derivados , Cantaridina/farmacologia , Cantaridina/uso terapêutico , Núcleo Celular/efeitos dos fármacos , Cromatina/metabolismo , Células Hep G2 , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , Componente 2 do Complexo de Manutenção de Minicromossomo , Componente 6 do Complexo de Manutenção de Minicromossomo
13.
ACS Nano ; 6(5): 4288-97, 2012 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-22494330

RESUMO

The tensile properties of carbon nanotube (CNT) fibers have been widely studied. However, the knowledge of their compressive properties is still lacking. In this work, the compressive properties of both pure CNT fibers and epoxy infiltrated CNT fibers were studied using the tensile recoil measurement. The compressive strengths were obtained as 416 and 573 MPa for pure CNT fibers and CNT-epoxy composite fibers, respectively. In addition, microscopic analysis of the fiber surface morphologies revealed that the principal recoil compressive failure mode of pure CNT fiber was kinking, while the CNT-epoxy composite fibers exhibited a failure mode in bending with combined tensile and compressive failure morphologies. The effect of resin infiltration on CNT fiber compressive properties, including the compressive strength and the deformation mode, is discussed. This work expands the knowledge base of the overall mechanical properties of CNT fibers, which are essential for their application in multifunctional composites.

14.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 29(6): 529-32, 2009 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-19702086

RESUMO

OBJECTIVE: To investigate the effects of Salvianolic acid B preconditioned endothelial progenitor cells (EPCs) on the Nkx2.5 and GATA-4 gene expressions at the early stage of cell differentiation of bone mesenchymal stem cells (BMSc) transplanted into infarcted myocardium, in order to find out the best synergism for co-transplantation of the two kinds of cells. METHODS: BMSc and EPCs of rats were isolated and cultured, and rats were modeled into acute myocardial infarction (AMI) by left coronary artery ligation. Then the EPCs preconditioned with different concentrations of Salvianolic acid B and BMSc or DMEM medium were implanted into heart ischemia area. Expressions of Nkx2.5 and GATA-4 mRNA expressions in myocardium were detected by Real-time RT-PCR 4 weeks later. RESULTS: Compared with those in the non-implanted model rats' myocardium, the gene expression of Nkx2.5 and GATA-4 mRNA were significantly higher in all the transplantation receptive groups, comparisons between the implanted groups showed that the highest value of expressions (2. 654 +/- 0.606 of Nkx2.5 and 1.573 +/- 0.372 of GATA-4) displayed in the group contained more EPCs, for 8-fold to BMSc in volume. CONCLUSION: BMSc can differentiate into cardiac muscle like cells, and condition of their differentiation is related with the degree of the internal environment improved.


Assuntos
Benzofuranos/farmacologia , Células Endoteliais/efeitos dos fármacos , Transplante de Células-Tronco Mesenquimais , Infarto do Miocárdio/terapia , Células-Tronco/efeitos dos fármacos , Animais , Células Cultivadas , Células Endoteliais/citologia , Células Endoteliais/transplante , Expressão Gênica/efeitos dos fármacos , Masculino , Infarto do Miocárdio/metabolismo , Miocárdio/metabolismo , Distribuição Aleatória , Ratos , Ratos Wistar , Transplante de Células-Tronco , Células-Tronco/citologia
15.
Asian J Androl ; 10(3): 447-54, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18385906

RESUMO

AIM: To study the clinical effects of a disposable circumcision device in treatment of male patients of different ages with either phimosis or excess foreskin. METHODS: One thousand two hundred patients between the age of 5 and 95 years underwent circumcision using this procedure in the 2-year period between October 2005 and September 2007. Of these cases, 904 had excess foreskin and 296 were cases of phimosis. RESULTS: In 96.33% of the cases the incision healed, leaving a minimal amount of the inner foreskin with no scarring and producing good cosmetic results. There were no incidents of device dislocation or damage to the frenulum. The average operative time was 2.5 min for excess foreskin, and 3.5 min for phimosis. During the 7 days of wearing the device, mild to moderate edema occurred in 10.08% of cases with excess foreskin and in 2.58% of those with phimosis. Edema in the frenulum was seen in 1.67% of patients, and only 0.67% had an infection of the incision. A total of 86.25% of patients reported pain due to penile erection. After removal of the device, 0.58% of the cases had minimal bleeding around the incision, and 2.42% had wound dehiscence. CONCLUSION: The new device can be applied to an overwhelming majority of patients with phimosis and excess foreskin. This technique is relatively simple to perform, and patients who underwent this surgery had very few complications. Antibiotics were not required and patients reported less pain than those who were circumcised using conventional methods. Circumcision with this device requires minimal tissue manipulation, and is quicker and safer than circumcision using conventional techniques.


Assuntos
Circuncisão Masculina/métodos , Procedimentos Cirúrgicos Minimamente Invasivos , Adolescente , Criança , Pré-Escolar , Humanos , Masculino , Dor Pós-Operatória
16.
Zhongguo Zhen Jiu ; 25(1): 3-6, 2005 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-16312041

RESUMO

Under guiding of correct thinking of scientific research, development of clinical scientific research of acupuncture and moxibustion is of important significance for promoting academic progresses of acupuncture and moxibustion, increasing clinical therapeutic effect of acupuncture and moxibustion. This paper probes thinking of clinical scientific research of acupuncture and moxibustion and puts forward the guiding principles, i.e. testing and verifying therapeutic effect, searching for laws, expounding mechanisms and guiding clinical practice.


Assuntos
Acupuntura , Moxibustão , Terapia por Acupuntura , Pesquisa Biomédica , Humanos
17.
Zhongguo Zhen Jiu ; 25(3): 217-20, 2005 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-16312937

RESUMO

OBJECTIVE: To observe effects of electroacupuncture of different frequencies on morphological changes of nervous tissues and electromyogram (EMG) of skeletal muscles in regeneration,so as to find more proper parameters of electroacupuncture. METHODS: The nerve regeneration chamber was built after the sciatic nerve was transected, and acupuncture was given at "Huantiao" (GB 30) , "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) at the affected side with different frequencies (5 Hz, 100 Hz), 30 min each time,once every other day, 3 time each week, for 20 weeks and a control group was set up for natural recovery. The nerve fibers were stained by silver dying and ultrastructures of nerve tissues were observed. EMG of gastrocnemius was determined by Biopac physiologic apparatus. RESULTS: Electroacupuncture could promote the recovery of form of nerve tissues after the transection of sciatic nerve and functions of de-innervated muscle tissues after lesion of sciatic nerve, and the therapeutic effect of electroacupuncture at 5 Hz was the best. CONCLUSION: Electroacupuncture is an important way of promoting the regeneration of the lessoned peripheral nerve.


Assuntos
Eletroacupuntura , Nervo Isquiático , Terapia por Acupuntura , Animais , Eletromiografia , Músculo Esquelético , Ratos , Ratos Sprague-Dawley
18.
Anal Sci ; 18(7): 751-5, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12137368

RESUMO

It has recently been reported that dopamine may show some biological activities in antitumor and cell apoptosis. We have thoroughly investigated the interaction between dopamine and DNA by CD, UV, fluorescence and electrochemical methods. The results of spectroscopic measurements have indicated that a binding event occurs in a dopamine-DNA system. Besides the electronstatic interaction between a negatively charged DNA molecule and a positively charged dopamine molecule, other binding modes, such as hydrogen-bond and intercalation may also exist in this system. The interaction parameters, including the equilibrium constant and binding numbers, were estimated by an electrochemical method based on the redox current and formal potentials. Both of the two calculation methods showed that the 1:1 type of complex was formed in the dopamine-DNA system and that its equilibrium constant was about 5.85 x 10(6) M(-1). Based on the results of UV, fluorescence and electrochemical experiments in the present study, dopamine may be employed as an effective probe for a DNA assay.


Assuntos
DNA/química , DNA/metabolismo , Dopamina/química , Dopamina/metabolismo , Animais , Dicroísmo Circular , Condutometria , Peixes , Ligação de Hidrogênio , Modelos Logísticos , Concentração Osmolar , Sais/farmacologia , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta
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