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1.
Eur Rev Med Pharmacol Sci ; 25(21): 6670-6677, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34787871

RESUMO

OBJECTIVE: Numerous studies show association of particular matter (PM) in air pollution with cardiovascular dysfunction, and increased morbidity and mortality. The main mechanisms of this adverse effect involve increasing oxidative stress, inflammatory responses, and genotoxicity. Several recent studies investigated the ability of PM2.5 to cause myocardial injury in animal models using various methods, such as intratracheal instillation, intraperitoneal injection or tail vein injection. The purpose of this study is to explore the PM2.5-induced myocardial inflammatory reaction in rats through the new technology of multi-functional aerosol concentration and enrichment system. MATERIALS AND METHODS: Thirty Wistar rats were divided into two groups, 15 in each group. In the exposure group, PM2.5 multi-functional aerosol concentration and enrichment system was used for PM2.5 online oral and nasal exposure (5 times a week, 4 hours exposure, for the duration of 3 months). Histopathological examination of the left ventricular myocardial tissue of both groups was done using hematoxylin and eosin (H&E) staining. Ultrastructural changes of the heart specimens were assessed using electron microscopy. The levels of CRP and ICAM-1 were detected by immunohistochemistry. RESULTS: Compared with the control group, myocardial tissue of the exposure group exhibited edema, widened myocardial space and infiltration of inflammatory cells. There was nuclear pyknosis, mitochondrial membrane and spinal fusion, rough endoplasmic reticulum expansion, degranulation and cell swelling in the exposed group. The area of CRP positive staining in the exposed group was 3.7-fold higher than that in the control group (p < 0.05), and the ICAM-1 positive staining area of the exposed group was 12-fold higher than that of the control group (p < 0.05). CONCLUSIONS: Prolonged exposure to PM2.5 inhalation promotes significant upregulation of ICAM-1 and CRP expression in myocardial tissues, ultrastructural alterations in myocardial cells, and influx of inflammatory cells.

3.
Br J Dermatol ; 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34741300

RESUMO

BACKGROUND: Tinea capitis is still common in developing countries, such as China. Its pathogen spectrum varies across regions and changes over time. OBJECTIVES: This study aimed to clarify the current epidemiological characteristics and pathogen spectrum of tinea capitis in China. METHODS: A multicentre, prospective descriptive study involving 29 tertiary hospitals in China was conducted. From August 2019 to July 2020, 611 patients with tinea capitis were enrolled. Data concerning demography, risk factors and fungal tests were collected. The pathogens were further identified by morphology or molecular sequencing when necessary in the central laboratory. RESULTS: Among all enrolled patients, 74.1% of the cases were 2- to 8-year-olds. The children with tinea capitis were mainly boys (56.2%) and more likely to have an animal contact history (57.4% vs. 35.3%, P = 0.012) and zoophilic dermatophyte infection (73.5%). The adults were mainly females (83.3%) and more likely to have anthropophilic agent infection (53.5%). The most common pathogen was zoophilic Microsporum canis (354, 65.2%), followed by anthropophilic Trichophyton violaceum (74, 13.6%). In contrast to the eastern, western and northeastern regions where zoophilic M. canis predominated, anthropophilic T. violaceum predominated in central China (69.2%, P < 0.0001), where the patients had the most tinea at other sites (20.3%) and dermatophytosis contact (25.9%) with the least animal contact (38.8%). Microsporum ferrugineum was the most common anthropophilic agent in the western area, especially in Xinjiang Province. CONCLUSIONS: Boys aged approximately 5 years were mainly affected. Dermatologists are advised to pay more attention to the different transmission routes and pathogen spectra in different age groups from different regions.

4.
Zhonghua Liu Xing Bing Xue Za Zhi ; 42(4): 569-574, 2021 Apr 10.
Artigo em Chinês | MEDLINE | ID: mdl-34814431

RESUMO

With the rapid changes in lifestyle, natural and social environment, the reproductive health status of couples in childbearing age continues to decline, and long-term outcomes of the rapidly increasing offspring conceived by assisted reproductive technology (ART) needs to be evaluated urgently. Therefore, the focus of research now needs to be extended from death and severe diseases to full life cycle and full disease spectrum. In order to meet the demand for such research, we launched the China National Birth Cohort (CNBC) study, an ongoing prospective and longitudinal study aiming to recruit 30 000 families underwent ART and 30 000 families with spontaneous pregnancies. Long-term follow-up programs will be conducted for both spouses and their offspring. Data of couples and their offspring, such as environmental exposure, reproductive history, psychological and behavioral status, will be collected during follow-up. Peripheral blood, urine, umbilical blood, follicular fluid, semen were also collected at different follow-up nodes. Based on high-quality data and biological samples, CNBC will play an extremely important supporting role and have a far-reaching impact on maternal and children's health care and reproductive health in China. This paper is exactly a brief introduction to the construction and basic design of CNBC.


Assuntos
Sangue Fetal , Técnicas de Reprodução Assistida , China/epidemiologia , Feminino , Humanos , Estudos Longitudinais , Gravidez , Estudos Prospectivos , Reprodução
5.
Zhonghua Liu Xing Bing Xue Za Zhi ; 42(4): 579-585, 2021 Apr 10.
Artigo em Chinês | MEDLINE | ID: mdl-34814433

RESUMO

Objective: To explore the effects of environmental, genetic factors as well as the interactions in early life on the short-term and long-term health of offspring and to systematically evaluate the pregnancy outcomes and health of offspring after birth between families with assisted reproductive technology (ART) conception and families with spontaneous conception. Methods: The China National Birth Cohort (CNBC), a multicenter prospective birth cohort study, includes both families with ART conception and families with spontaneous conception. Since 2016, CNBC has recruited families from 24 hospitals located in 12 provinces, municipalities and autonomous regions throughout China. Information and biospecimens were collected before ART treatment, embryo transfer, at early, second, third trimester and delivery, and at 42 days, 6, 12 and 36 months after birth. Results: By June 2020, CNBC had included 27 044 families with ART conception and 29 589 families with spontaneous conception. The majority of the participants are urban residents. Among the families with ART conception, 65.5% of the men and 63.7% of the women had college degrees or higher. The mean age distribution of men and women was (33.83±5.52) and (32.38±4.67) years. 83.2% of women were primiparas, and the prevalence rates of current regular smokers and current alcohol drinkers were 0.8% and 2.1% in women. Among the families with spontaneous conception, 81.5% of the men and 86.5% of the women had college degrees or higher. The mean age distribution of men and women was (32.06±5.09) and (30.40±4.27) years. 67.2% of women were primiparas, and the prevalence rates of current regular smokers and current alcohol drinkers were 0.1% and 2.2% in women. The baseline characteristics were different between the families with ART conception and spontaneous conception in different regions. Conclusion: CNBC provides a powerful and rich resource in studying the impact of genetic, environmental factors and interactions in early life and ART treatment on the health of offspring after birth.


Assuntos
Resultado da Gravidez , Técnicas de Reprodução Assistida , Estudos de Coortes , Feminino , Humanos , Masculino , Gravidez , Terceiro Trimestre da Gravidez , Estudos Prospectivos
6.
Zhonghua Liu Xing Bing Xue Za Zhi ; 42(7): 1200-1204, 2021 Jul 10.
Artigo em Chinês | MEDLINE | ID: mdl-34814531

RESUMO

Objective: To understand the status of tobacco use and related influencing factors in population in Shandong province. Methods: Stratified multi-stage cluster sampling was used to select respondents from 216 villages (communities) of 36 districts (counties) in Shandong province. Influencing factors on smoking were analyzed by logistic regression model. Results: The adequate sample size was 6 271 participants. After complex weighted, the current smoking prevalence was 23.93%,45.58% in males and 1.18% in females. The Public's smoking rates varied widely among different groups in the population. 45-64 age group had the highest smoking rate (25.07%). Smoking rates were substantially different in education levels, with the highest in junior high school (28.94%). The rate was higher in the rural area (24.98%) than that in the urban areas (23.08%). The average daily smoking rate was 20.23%. The average age of initiating smoking was 21.21 years. The average daily cigarette intake was 16.31 cigarettes. Among all the former and current smokers, the quitting rate was 20.79%. Multiple logistic regression model analysis showed that gender, age, occupation, region, and health knowledge score were correlated with smoking behavior. The current smoking rate of men was much higher than that of women (OR=49.625, 95%CI: 37.832-65.093). The current smoking rate in 45-64 age group was higher than that in the 15-24 age group (OR=1.830, 95%CI: 1.048-3.194). The current smoking rate of medical (OR=0.403, 95%CI: 0.187-0.866) and retired personnel (OR=0.648, 95%CI: 0.481-0.873) were lower than those engaging in agriculture, forestry, animal husbandry and the fishery ,respectively. The prevalence of residents living in the central part showed lower rate on current smoking than that in the eastern region (OR=0.724, 95%CI: 0.606-0.865). The current smoking prevalence of smoke hazard in 1-3 score group was higher than that in the group with 4-6 score (OR=1.432, 95%CI: 1.240-1.654). Conclusions: Smoking rate in adults in Shandong remained stable and at a high level. Comprehensive intervention measures such as tobacco control and health education should be carried out to reduce the smoking rate.


Assuntos
Abandono do Hábito de Fumar , Poluição por Fumaça de Tabaco , Adulto , Feminino , Humanos , Masculino , Fumar/epidemiologia , Inquéritos e Questionários , Uso de Tabaco/epidemiologia , Adulto Jovem
9.
Int J Radiat Oncol Biol Phys ; 111(3S): e259, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701034

RESUMO

PURPOSE/OBJECTIVE(S): The management of oligometastatic bladder cancer is unclear. Prior data implies site and burden of metastases vary in prognoses. This study evaluates treatment trends, examines the effect of local therapy on survival, and explores if immunotherapy influences outcomes in patients with de novo oligometastatic bladder urothelial carcinoma. MATERIALS/METHODS: A retrospective analysis of the National Cancer Database (NCDB) was performed by identifying bladder urothelial carcinoma patients with metastases (M1 stage) at diagnosis between 2004 and 2016. Local therapy was classified as high-intensity [HT] (i.e., definitive surgery, radiation [RT] dose > 50 Gy) or low-intensity [LT] (i.e., non-definitive surgery, RT dose < 50 Gy). Chemotherapy (CT) was classified relative to local therapy as cytoreductive or consolidative. Metastatic burden was determined by cumulative summation of number of metastatic sites involved (i.e., brain, bone, liver, and lung). Clinicopathologic characteristics were analyzed by descriptive statistics and a Chi-square test was used for comparisons across metastatic disease burden. Univariable (UVA) Kaplan-Meier analysis was used to estimate overall survival (OS). A multivariable Cox regression model was used to estimate the risk of poor survival by treatment modality. Also, effect-modification was assessed between treatment intensity and systemic therapy type. RESULTS: A total of 11,998 patients were identified with metastases at diagnosis. The median age was 72 years (IQR: 62-80), the majority were Caucasian (87.5%) and male (71.2%). The median follow-up time was 5.5 months (IQR: 2.27-12.62). Patients were treated with definitive surgery (n = 849; 7.1%), perioperative CT (n = 404; 3.4%), CT alone (n = 4,030; 33.6%), RT alone (n = 1,026; 8.6%), CTRT (n = 1,451; 12.1%) or no therapy (n = 3,343; 33.6%). HT and LT were given to 1,083 (9%) and 8,867 (73.9%) patients, respectively. Differences were noted in median OS among treatment groups, such as perioperative CT (OS 16.4 months) or CTRT (> 50 Gy) (12.4 months); P < 0.001. HT vs LT improved OS (10.2 vs 5.7 months; P < 0.001). Consolidative vs cytoreductive therapy improved OS (P < 0.001), but within HT/LT comparisons, sequencing was only significant in HT (16.5 vs 12.7 months; P < 0.001), which was confirmed by interaction analysis. A total of 4,315 patients had site-specific data: bone (n = 1,486), lung (n = 1,175), liver (n = 525) and brain (n = 70). Differences were noted in OS between sites (P < 0.001) with liver and brain having the worst OS. Metastatic burden was also associated with OS (P < 0.001). Immunotherapy was given to 174 patients, which improved OS (12.2 vs 5.7 months; P < 0.001), was independent of metastatic burden, and was associated with 41% reduction in mortality (HR: 0.59, 95% CI: 0.49-0.69; P < 0.001). CONCLUSION: This population-based study suggests local aggressive therapy, either surgical or RT (> 50 Gy) is associated with an improved OS benefit in patients with de novo metastatic bladder urothelial carcinoma.

10.
Int J Radiat Oncol Biol Phys ; 111(3S): e91, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701997

RESUMO

PURPOSE/OBJECTIVE(S): Artificial intelligence (AI) driven tools have been maturing in automating the radiation treatment planning process. To prepare for clinical deployment of these tools, it is essential to understand their robustness in clinical scenarios. This study works to fill the existing gap between research initiated and clinically ready AI tools by investigating a clinical assessment approach based on phantom design and planning complexity simulation. The hypothesis is that AI tool assessment program provides more clinically relevant and comprehensive evaluations beyond typical model validation studies. MATERIALS/METHODS: A cylindrical digital phantom was designed in the treatment planning system with an axial diameter of 30 cm and length of 18 cm. The phantom contains key structures involved in pancreas SBRT including the PTV25Gy, PTV33Gy, C-loop, stomach, bowel and liver with their base shape and volume representing the average of 100 clinical SBRT patients. Phantom cases were synthesized to mimic real-life anatomical variations and overlaps through displacement, expansion, and rotation of PTVs and OARs. This study involved a total of 32 simulated cases to test a broad range of planning scenarios. A previously developed deep learning based automatic planning tool was assessed in this study. This AI tool is composed of two deep neural networks (NNs) which predict beam dose and fluence maps sequentially. The goal of treatment planning was to deliver 25 Gy to PTV25 and 33 Gy to PTV33 in 5 fractions via simultaneous integral boost (SIB) while limiting luminal OAR max dose to below 29 Gy. The AI-plan's quality was analyzed against the clinical evaluation criteria, which include PTV V100%, luminal OAR max dose using Dmax and D0.03cc. The passing rate of key clinical criteria were collected to quantify overall robustness. RESULTS: For all scenarios, the mean PTV25 V25Gy of the AI plans was 96.7% while mean PTV33 V33 Gy was 82.2%. Large variation (16.3%) in PTV33 V33 Gy was observed due to anatomical variations, i.e., proximity of luminal structures to PTV33. Mean max dose was 28.55, 27.68, and 24.63 Gy for the C-loop, bowel and stomach respectively. Using D0.03cc as a surrogate for max dose, the value was 28.03, 27.12, and 23.84 Gy for the same respective structures. A max dose constraint of 29 Gy was achieved for 81.3% cases for the C-loop and stomach, and 78.1% for the bowel. Using D0.03cc as a surrogate for max dose, the passing rate was 90.6% for the C-loop and 81.3% for both the bowel and stomach. CONCLUSION: The results showed promising robustness of AI planning tool for pancreas SBRT, providing important evidence of its readiness for clinical implementation. The established approach could guide the robustness testing and other clinical assessments of AI based treatment planning tools in general, which is an important component for safe clinical implementation.

12.
Physiol Res ; 70(5): 671-685, 2021 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-34505522

RESUMO

Cancer is a complex, multifactorial disease that modern medicine ultimately aims to overcome. Downstream of tyrosine kinase 2 (DOK2) is a well-known tumor suppressor gene, and a member of the downstream protein DOK family of tyrosine kinases. Through a search of original literature indexed in PubMed and other databases, the present review aims to extricate the mechanisms by which DOK2 acts on cancer, thereby identifying more reliable and effective therapeutic targets to promote enhanced methods of cancer prevention and treatment. The review focuses on the role of DOK2 in multiple tumor types in the lungs, intestines, liver, and breast. Additionally, we discuss the potential mechanisms of action of DOK2 and the downstream consequences via the Ras/MPAK/ERK or PI3K/AKT/mTOR signaling pathways.

13.
Br Poult Sci ; : 1-10, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34406094

RESUMO

1. The role of melanoma differentiation-associated protein 5 (MDA5) in infectious bursal disease virus (IBDV)-induced autophagy was studied in chicken embryos.2. Chicken embryo fibroblasts (CEF) were used as the research model and small interfering RNA (siRNA), western blot, indirect enzyme-linked immunosorbent assay (ELISA), real-time fluorescence quantitative polymerase chain reaction (PCR) and transmission electron microscopy were used to detect autophagy, IBDV replication, CEF damage, and activation of both MDA5 and its signalling pathway.3. The results showed that CEF infected with IBDV activated the intracellular MDA5 signalling pathway and caused autophagy via inactivation of the AKT/mTOR pathway. While autophagy promotes IBDV proliferation, MDA5 weakens IBDV-induced CEF autophagy thus inhibiting IBDV replication and protecting CEF cells.4. The results indicated that chMDA5 can be activated by IBDV and attenuate CEF autophagy caused by IBDV infection, thereby inhibiting IBDV replication. This study provided a foundation for further exploring the relationship between viruses, autophagy and the pathogenic mechanism of the MDA5 pathway involved in IBDV.

14.
Zhonghua Bing Li Xue Za Zhi ; 50(7): 740-744, 2021 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-34405607

RESUMO

Objective: To investigate the expression and diagnostic value of SS18-SSX fusion-specific antibody and SSX C-terminal antibody in synovial sarcoma (SS). Methods: Immunohistochemical (IHC) EnVision method was used to detect the expression of SS18-SSX fusion-specific antibody and SSX C-terminal antibody in 51 genetically confirmed cases of SS and 94 non-SS tumors diagnosed at Nanjing Jinling Hospital from August 2013 to December 2020. Results: IHC staining for SS18-SSX fusion-specific antibody revealed strongly diffuse nuclear staining in 48 of 51 (48/51, 94.1%) SS cases, whereas none of the 94 non-SS tumors showed any staining. IHC staining for SSX C-terminal antibody showed strongly diffuse nuclear staining in all 51 (51/51, 100%) SS cases; six of the 94 (6/94, 6.4%) non-SS tumors showed variable staining, including two cases each of leiomyosarcoma and fibrosarcoma, and one case each of malignant peripheral nerve sheath tumor and embryonal rhabdomyosarcoma. The sensitivity and specificity of SS18-SSX fusion-specific antibody in diagnosing SS were 94.1% and 100% and these of SSX C-terminal antibody were 100% and 93.6%, respectively. Conclusions: SS18-SSX fusion-specific antibody and SSX C-terminal antibody are highly sensitive and specific markers for SS. Immunohistochemistry using these antibodies may replace FISH or molecular genetic testing in most cases.


Assuntos
Sarcoma Sinovial , Anticorpos , Humanos , Imuno-Histoquímica , Proteínas de Fusão Oncogênica/genética , Sarcoma Sinovial/diagnóstico , Sarcoma Sinovial/genética , Sensibilidade e Especificidade
15.
Zhonghua Yu Fang Yi Xue Za Zhi ; 55(8): 965-972, 2021 Aug 06.
Artigo em Chinês | MEDLINE | ID: mdl-34445834

RESUMO

Objective: To explore the effect of different calcium intakes on metabolism in healthy adults with traditional Chinese dietary pattern. Methods: A total of 64 college students (32 males and 32 females) were recruited from the School of Public Health, Harbin Medical University from September to October 2015. A prospective, randomized, double-blind, placebo-controlled trial design (RCT trial) was used to strictly control the diet of the volunteers through meal preparation, so that the background of the experimental and the control group was exactly the same. In our study, 64 healthy adults were randomly divided into two groups (calcium supplementation and control). The two groups were given the same diet every day. One group was given 400 mg/d of calcium supplementation, which was close to the recommended intake in China (800 mg/d); the other group was given pacebo, the average calcium intake was close to the average intake of Chinese (400 mg/d), calcium intervention lasted for 35 days. Serum and urine samples from pre-intervention, mid-intervention, and post-intervention were collected for metabolomics studies and detection of serum hormone levels and biochemical indicators. PCA and PLS-DA were used to compare the differences in serum and urine metabolites. After adjusting for age and BMI, the differences of hormones [parathyroid hormone, 1, 25(OH)2D3] and biochemical indicators were compared by repeated measurement variance analysis. Results: The 64 subjects were aged 23-28 years, including 32 males and 32 females. The baseline characteristics of the calcium supplementation group and the control group were balanced. The results showed that there were no significant differences in blood and urine metabolites, metabolic hormone [parathyroid hormone, 1, 25(OH)2D3] levels and biochemical indicators(serum Ca, serum P, glycolipid metabolism and hepatorenal function indicators)between the calcium supplementation group and control group at each time point throughout the trail (P>0.05). Conclusion: No health hazards associated with calcium deficiency was observed in healthy adults in the short term at current calcium intake level (300-400 mg/d).


Assuntos
Cálcio na Dieta , Cálcio , Adulto , Dieta , Suplementos Nutricionais , Feminino , Humanos , Masculino , Hormônio Paratireóideo , Estudos Prospectivos , Vitamina D
16.
Zhonghua Gan Zang Bing Za Zhi ; 29(7): 711-715, 2021 Jul 20.
Artigo em Chinês | MEDLINE | ID: mdl-34371545

RESUMO

Non-alcoholic steatohepatitis (NASH) is an important link for the progression of metabolic-related fatty liver disease to end-stage liver disease such as cirrhosis and hepatocellular carcinoma, which seriously endangers human health. NASH pathogenesis is complex, and involves the interaction between hepatic parenchymal and non-parenchymal cells (NPCs), sinusoidal endothelial cells, Kupffer cells, hepatic stellate cells, and so on. Herein, the relevant research progress of NPCs in the pathogenesis of NASH is reviewed in order to further understand the role of NPCs in NASH.


Assuntos
Neoplasias Hepáticas , Hepatopatia Gordurosa não Alcoólica , Células Endoteliais , Hepatócitos , Humanos , Fígado
17.
Sci Total Environ ; 798: 149289, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34340085

RESUMO

Reverse osmosis (RO) is being used in many water reclamation facilities to produce high quality water that can be reused for different purposes. As a part of the RO process, a reject stream is produced as the reverse osmosis concentrate (ROC), which contains elevated levels of contaminants compared to the source water. Effective treatment and safe disposal of ROC via cost-effective means is very challenging. This study aims to develop a robust microbubble ozonation-biological process for industrial ROC treatment with a target effluent chemical oxygen demand (COD) lower than 60 mg/L. As compared to macrobubble ozonation, microbubble ozonation exhibited better ozone dissolution and 29% higher COD removal efficiency with the same ozone dosage. Under the optimum operating conditions with ozone dosage of 30 mg/L, ROC natural pH of 8.67 and ozonation duration of 1 h, microbubble ozonation achieved 42% COD removal efficiency while increasing the BOD5/COD ratio (ratio of biological oxygen demand over 5 days to the corresponding chemical oxygen demand) in ROC from 0.042 to 0.216. A biological activated carbon (BAC) column with an empty bed contact time (EBCT) of 120 min was combined with microbubble ozonation for continuous ROC treatment. Over the 100-day operation, the combined system performed consistent organics removal with an average effluent COD of 45 mg/L. Both LC-OCD data and fluorescence EEM spectra confirmed humic substances were the dominant organic species in ROC. Ozone pre-treatment could achieve significant removal of humic substances in raw ROC. ATP analysis found that ozone pre-treatment enhanced BAC biofilm activity by around 5 folds. 5 min acute toxicity assessment with Aliivibrio fischeri showed 4 times reduction of bioluminescence inhibition in ozone treated ROC. From the environmental point of view, Life cycle assessment (LCA) results demonstrated that Ozone-BAC system had significant environmental burdens on climate change and human toxicity due to the electricity production process. These environmental impacts can be mitigated by optimizing the ozonation process with reduced ozone dosage or utilizing renewable energy sources for electricity generation.


Assuntos
Ozônio , Poluentes Químicos da Água , Carvão Vegetal , Meio Ambiente , Humanos , Microbolhas , Osmose , Poluentes Químicos da Água/análise
18.
Beijing Da Xue Xue Bao Yi Xue Ban ; 53(4): 716-720, 2021 Aug 18.
Artigo em Chinês | MEDLINE | ID: mdl-34393234

RESUMO

OBJECTIVE: To investigate the role of rebamipide in the treatment of acute gout arthritis rats induced by monosodium urate (MSU) crystal. METHODS: Forty-two male rats were randomly divided into three groups (n=14). Group A was treated with oral rebamipide, group B with oral colchicine, and group C with oral placebo. The rats were monitored for the induction of arthritis with clinical manifestations and pathological changes, and the levels of interleukin (IL)-1ß、IL-6、IL-10, and tumor necrosis factor (TNF)-α in serum were measured. RESULTS: In group C, the clinical score and swelling index reached the maximum in 24 h, and then gradually decreased to 72 h. After 24 h of model induced, the clinical scores in group C were significantly higher than those in group A and group B [2 (1-3) vs. 0 (0-1) vs. 1 (0-2), P < 0.01], the swelling indexes in group C were significantly higher than those in group A and group B [0.36 (0.16-0.52) vs. 0.11 (0-0.20) vs. 0.12 (0-0.16), P < 0.01]. Histologically, after 24 h of model induced, there was a large number of neutrophil infiltration in the synovium of group C [scale score: 4 (2-4)], and there was no significant inflammatory cell infiltration in group A [1 (0-2)] and group B [1 (0-2)], the difference was statistically significant (P < 0.001). After 24 h of model induced, the levels of IL-1ß, IL-6, IL-10, and TNF-α in serum of group C were significantly higher than those in group A and B [IL-1ß: (41.86±5.72) vs. (27.35±7.47) vs. (27.76±5.28) ng/L, IL-6: (1 575.55±167.11) vs. (963.53±90.22) vs. (964.08±99.31) ng/L, IL-10: (37.96±3.76) vs. (21.68±4.83) vs. (16.20±2.49) ng/L, TNF-α: (21.32±1.34) vs. (15.82±2.54) vs. (17.35±7.47) µg/L, P < 0.001]. CONCLUSION: Rebamipide has a protective effect on acute gout arthritis rats induced by MUS crystals.


Assuntos
Artrite Gotosa , Quinolonas , Alanina/análogos & derivados , Animais , Artrite Gotosa/induzido quimicamente , Artrite Gotosa/tratamento farmacológico , Interleucina-1beta , Masculino , Ratos , Ácido Úrico
19.
Zhonghua Liu Xing Bing Xue Za Zhi ; 42(8): 1330-1335, 2021 Aug 10.
Artigo em Chinês | MEDLINE | ID: mdl-34404153

RESUMO

This paper summarizes the basic principles and models of early warning for infectious disease outbreaks, introduces the early warning systems for infectious disease based on different data sources and their applications, and discusses the application potential of big data and their analysing techniques, which have been studied and used in the prevention and control of COVID-19 pandemic, including internet inquiry, social media, mobile positioning, in the early warning of infectious diseases in order to provide reference for the establishment of an intelligent early warning mechanism and platform for infectious diseases based on multi-source big data.

20.
Zhonghua Fu Chan Ke Za Zhi ; 56(7): 458-466, 2021 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-34304437

RESUMO

Objective: To evaluate the value of whole exome sequencing (WES) in prenatal clinical application. Methods: A total of 1 152 cases of congenital abnormal [including structural malformation, nuchal translucency (NT) thickening and intrauterine growth restriction] with traditional prenatal diagnosis [including G-band karyotype analysis and chromosome microarray analysis (CMA)] negative were analyzed. The congenital abnormal fetuses were divided into retrospective group and prospective group according to the time of WES detection, that is whether the pregnancy termination or not. According to the specific location of fetal malformation and their family history, the cohort was divided into subgroups. The clinical prognosis of all fetuses were followed up, and the effect of WES test results on pregnancy decision-making and clinical intervention were analyzed. According to the follow-up results, the data of fetuses with new phenotypes in the third trimester or after birth were re-analyzed. Results: Among 1 152 families who received WES, 5 families were excluded because of nonbiological parents. Among the remaining 1 147 families, 152 fetuses obtained positive diagnosis (13.3%,152/1 147), including 74 fetuses in the retrospective group (16.1%,74/460) and 78 fetuses in the prospective group (11.4%,78/687). In fetuses with negative CMA and G-band karyotype analysis results but new phenotypes in the third trimester or after birth, the positive rate by WES data re-analysis was 4.9% (8/163). A total of 34 (21.3%, 34/160) fetuses were directly affected by the corresponding positive molecular diagnosis. Among 68 cases of live births with diagnostic variation grade 4, 29 cases (42.7%, 29/68) received appropriate medical intervention through rapid review of WES results. Conclusions: WES could increase the detection rate of abnormal fetuses with negative G-banding karyotype analysis and CMA by 13.3%. Prenatal WES could guide pregnancy decision-making and early clinical intervention. It might be an effective strategy to pay attention to the special follow-up of the third trimester and postnatal fetus and to re-analyze the WES data.


Assuntos
Anormalidades Congênitas , Diagnóstico Pré-Natal , Anormalidades Congênitas/diagnóstico , Anormalidades Congênitas/genética , Feminino , Feto/diagnóstico por imagem , Humanos , Medição da Translucência Nucal , Gravidez , Estudos Prospectivos , Estudos Retrospectivos , Ultrassonografia Pré-Natal , Sequenciamento Completo do Exoma
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