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1.
J Cell Mol Med ; 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33951295

RESUMO

The purpose of this study is to investigate the role of microRNA-125b (miR-125b) and its mechanism in spinal cord injury (SCI) by targeting Smurf1. After loss- and gain-function approaches were conducted in SCI rat models and neural stem cells (NSCs) isolated from foetal rats, the Basso-Beattie-Bresnahan (BBB) score was calculated, and related protein expression was determined by Western blot analysis and cell apoptosis by TUNEL staining. NSC viability was detected by CCK-8, migration abilities by Transwell assay and apoptosis by flow cytometry. The relationship between miR-125b, Smurf1 and KLF2 was evaluated by dual-luciferase reporter gene experiments, Co-IP and in vivo ubiquitin modification assays. Inhibition of miR-125b and KLF2 and the up-regulation of Smurf1 and ATF2 were observed in SCI rats. BBB scores were elevated, the expression of Nestin, NeuN, GFAP, NF-200 and Bcl-2 protein was enhanced but that of Bax protein was reduced, and cell apoptosis was inhibited in SCI rats after up-regulating miR-125b or silencing ATF2. Smurf1 was a target gene of miR-125b, which promoted KLF2 degradation through its E3 ubiquitin ligase function, and KLF2 repressed the expression of ATF2 in NSCs. The results in vivo were replicated in vitro. miR-125b overexpression promotes neurological function recovery after SCI.

2.
Pediatr Neurosurg ; : 1-8, 2021 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-33946073

RESUMO

INTRODUCTION: Oculomotor paresis with cyclic spasms (OPCS) is a rare disorder in which the muscles innervated by the third cranial nerve undergo alternating rhythmic spasm and paralysis. This disease is usually noticeable at birth or developing during the first year of life. To date, most of the OPCS cases reported in the English-language literature were Caucasians. CASE PRESENTATION: In this study, we review OPCS patients published in China's high-quality journals and report 1 new case encountered in our clinic. The clinical characteristics of 29 Chinese OPCS patients are summarized. Our review demonstrates the same trend in the analysis values for the onset age, pupil, lid, eye movement, time of cycles, and preferentially involved side in Chinese OPCS patients in comparison with previous reviews. Moreover, we review a case combined with Marcus Gunn Syndrome, a case with rapid relief under oral carbamazepine treatment, and cases with atypical patterns of cyclic changes. DISCUSSION: Our study may broaden the current knowledge and phenotypic spectrum of OPCS.

4.
Genes (Basel) ; 12(3)2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33810139

RESUMO

Cell-free fetal DNA (cffDNA) from maternal plasma has made it possible to develop noninvasive prenatal paternity testing (NIPPT). However, most studies have focused on customized single nucleotide polymorphism (SNP) typing systems and few have used conventional short tandem repeat (STR) markers. Based on massively parallel sequencing (MPS), this study used a widely-accepted forensic multiplex assay system to evaluate the effect of noninvasive prenatal paternity testing with a combination of well-established SNP and STR markers. Using a ForenSeq DNA Signature Prep Kit, NIPPT was performed in 17 real parentage cases with monovular unborn fetuses at 7 to 24 gestational weeks. Different analytical strategies for the identification of paternally inherited allele (PIA) were developed to deal with SNPs and STRs. Combined paternity index (CPI) for 17 real trios as well as 272 unrelated trios was calculated. With the combination of SNPs and A-STRs, 82.35% (14/17), 88.24% (15/17), 94.12% (16/17), and 94.12% (16/17) of real trios could be accurately determined when the likelihood ratio (LR) threshold for paternity inclusion was set to 10,000, 1000, 100, and 10, respectively. This reveals that simultaneous surveys of SNP and STR markers included in the ForenSeq DNA Signature Prep Kit offer a promising method for NIPPT using MPS technology.

5.
Pest Manag Sci ; 2021 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-33837648

RESUMO

BACKGROUND: Plutella xylostella (L.) is a serious worldwide pest that feeds on cruciferous plants and has evolved resistance to different classes of insecticides used for its control, including chlorantraniliprole. ATP-binding cassette (ABC) transporters, constituting the largest transport family in organisms, are involved in phase III of the detoxification process and may play important roles in insecticide resistance. RESULTS: A total of 15 ABC transporter transcripts from subfamily G were identified in P. xylostella based on the latest DBM genome. Synergism studies showed that treatment with verapamil, a potent inhibitor of ABC transporters, significantly increased the toxicity of chlorantraniliprole against larvae of two chlorantraniliprole-resistant P. xylostella populations (NIL and BL). ABCG2, ABCG5, ABCG6, ABCG9, ABCG11, ABCG14 and ABCG15 were significantly overexpressed in NIL and BL compared with the susceptible population (SS), and ABCG1, ABCG6, ABCG8, ABCG9, ABCG14 and ABCG15 were significantly upregulated after treatment with the LC50 of chlorantraniliprole in SS. Subsequently, ABCG6, ABCG9 and ABCG14, which were overexpressed in both NIL and BL and could be induced in SS, were chosen for functional study. RNAi-mediated knockdown of each of the three ABCGs significantly increased the sensitivity of larvae to chlorantraniliprole. These results confirmed that overexpression of ABCG6, ABCG9 and ABCG14 may contribute to chlorantraniliprole resistance in P. xylostella. CONCLUSION: Overexpression of some genes in the ABCG subfamily is involved in P. xylostella resistance to chlorantraniliprole. These results may help to establish a foundation for further studies investigating the role played by ABC transporters in chlorantraniliprole resistance in P. xylostella or other insect pests.

6.
J Forensic Sci ; 2021 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-33885147

RESUMO

For the past two to three decades, forensic DNA evidence has been analyzed with a limited number of short tandem repeats (STRs), and these STRs are usually assumed to be independent for statistical calculations. With the development and implementation of the MPS technologies, more autosomal markers, both single nucleotide polymorphisms (SNPs) and STRs, can be analyzed. A number of these markers are physically very close to each other, and it may not be appropriate to assume all these markers are genetically unlinked or in linkage equilibrium. In this study, publicly accessible genomic data from five representative populations were used to evaluate the genetic linkage and linkage disequilibrium (LD) between autosomal markers represented in six major commercial panels (in total, 362 markers). Among the 3041 syntenic marker pairs, 1524 pairs had sex-average genetic distances <50 cM, and thus, these marker pairs can be considered as genetically linked. Among the 143 marker pairs with physical distances <1 Mb, 19 LD haplotype blocks (comprising 39 SNPs in total) were detected for at least one of the tested populations. Statistical methods for interpreting linked markers and/or markers in LD were suggested for various case scenarios.

7.
Sci Rep ; 11(1): 7414, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33795706

RESUMO

Total dissolved gas (TDG) supersaturation, which occurs during dam spilling, may result in fish bubble disease and mortality. Many studies have been conducted to identify the factors pertaining to TDG generation, such as the spilling discharge and tailwater depth. Additionally, the energy dissipation efficiency should be considered due to its effect on the air entrainment, which influences the TDG generation process. According to the TDG field observations of 49 cases at Dagangshan and Xiluodu hydropower stations, the TDG was positively related to the energy dissipation efficiency, tailwater depth and discharge per unit width. A correlation between the generated TDG level and these factors was established. The empirical equations proposed by the USACE were calibrated, and the TDG level estimation performance was compared with the established correlation for 25 spillage cases at seven other dams. Among the considered cases, the standard error of the TDG estimation considering the energy dissipation efficiency was 5.7%, and those for the correlations obtained using the USACE equations were 13.0% and 10.0%. The findings indicated that the energy dissipation efficiency considerably influenced the TDG level, and its consideration helped enhance the precision of the TDG estimation. Finally, the generality of this approach and future work were discussed.

8.
Eur J Pharm Sci ; 162: 105829, 2021 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-33819624

RESUMO

This work aims to synthesize two novel 1-MT (1-Methyl-DL-tryptophan) grafted CMCS (carboxymethyl chitosan) polymer prodrugs CMCS-amido-1-MT and CMCS-ester-1-MT, and to further manufacture their nanoparticles for potential biomedical applications. The polymeric prodrugs are prepared by three-step chemical synthesis. The chemical structure of drugs is confirmed by FTIR and 1H-NMR. The drug loadings of the CMCS-amido-1-MT NPs and CMCS-ester-1-MT NPs are 11.43% and 10.18%, respectively. The surface morphology of the nanoparticles is spherical or nearly spherical, while the surface is smooth and the size distribution is uniform. The average particle size is both about 200 nm, while the polydispersity index is both about 0.15. The nanoparticles have a negative charge on the surface. The particle size and its distribution change little, when the two nanoparticles are tested in the simulated blood pH environment for 7 days. However, only the CMCS-ester-1-MT nanoparticles are pH-sensitive. The cell toxicity of the CMCS-ester-1-MT nanoparticles and the original drug are both in a dose- and time-dependent manner, while the nanoparticles enter cells by endocytosis. In ECA109 cells, the CMCS-ester-1-MT nanoparticles and the original drug both induce the apoptosis. CMCS-ester-1-MT NPs can activate the ATF4/CHOP pathway in endoplasmic reticulum stress, and achieve cancer suppression through mitochondrial-related apoptosis.

9.
Plant Signal Behav ; : 1916702, 2021 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-33896377

RESUMO

Long non-coding RNAs (lncRNAs) in plants are emerging as new players in biotic stress responses. Pathogen-associated lncRNAs have been broadly identified and functionally characterized in multiple species. However, herbivore-responsive lncRNAs in plants are poorly investigated. Our recent study revealed that lncRNAs also play roles in plant defense against herbivores in wild tobacco. Here, we identified armyworm (AW)-elicited lncRNAs in monocot rice by employing a similar approach. A total of 238 lncRNAs were found to be differentially expressed (DE) in AW-treated plants relative to control plants. The cis effect of these DE lncRNAs was predicted. Interestingly, one DE lncRNA was identified from the antisense transcripts of the jasmonate ZIM-domain gene JAZ10.

10.
Materials (Basel) ; 14(6)2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33803767

RESUMO

Bead size and shape are important considerations for industry design and quality detection. It is hard to deduce an appropriate mathematical model for predicting the bead geometry in a continually changing welding process due to the complex interrelationship between different welding parameters and the actual bead. In this paper, an artificial neural network model for predicting the bead geometry with changing welding speed was developed. The experiment was performed by a welding robot in gas metal arc welding process. The welding speed was stochastically changed during the welding process. By transient response tests, it was indicated that the changing welding speed had a spatial influence on bead geometry, which ranged from 10 mm backward to 22 mm forward with certain welding parameters. For this study, the input parameters of model were the spatial welding speed sequence, and the output parameters were bead width and reinforcement. The bead geometry was recognized by polynomial fitting of the profile coordinates, as measured by a structured laser light sensor. The results showed that the model with the structure of 33-6-2 had achieved high accuracy in both the training dataset and test dataset, which were 99% and 96%, respectively.

11.
J Agric Food Chem ; 69(17): 5144-5154, 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33881846

RESUMO

Cystatins are proteins, which inhibit cysteine proteases, such as papain. In this study, the 336-bp cystatin C gene (family II, HmCysC) of silver carp (Hypophthalmichthys molitrix) was cloned and expressed in Escherichia coli BL21 (DE3). HmCysC encodes the mature peptide of cystatin C (HmCystatin C), with 111 amino acids. A typical QXXXG motif was found in HmCystatin C and it formed a cluster with Cyprinus carpio and Danio rerio cystatin C in the phylogenetic tree. Quantitative real-time polymerase chain reaction analysis indicated that HmCysC was transcribed at different levels in five tested tissues of silver carp. Following purification with Ni2+- nitrilotriacetic acid agarose affinity chromatography, HmCystatin C displayed a molecular weight of 20 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis. Purified HmCystatin C had strong inhibitory effects toward the proteolytic activity of papain. Immunochemical staining with anti-HmCystatin C antibody showed that HmCystatin C was widely distributed in silver carp tissues. These results collectively demonstrated the properties of HmCystatin C, providing information for further studies of cystatins from fish organisms.

12.
Chin Med J (Engl) ; 134(8): 944-953, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33813510

RESUMO

BACKGROUND: Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly spread throughout the world. In this study, we aimed to identify the risk factors for severe COVID-19 to improve treatment guidelines. METHODS: A multicenter, cross-sectional study was conducted on 313 patients hospitalized with COVID-19. Patients were classified into two groups based on disease severity (nonsevere and severe) according to initial clinical presentation. Laboratory test results and epidemiological and clinical characteristics were analyzed using descriptive statistics. Univariate and multivariate logistic regression models were used to detect potential risk factors associated with severe COVID-19. RESULTS: A total of 289 patients (197 nonsevere and 92 severe cases) with a median age of 45.0 (33.0, 61.0) years were included in this study, and 53.3% (154/289) were male. Fever (192/286, 67.1%) and cough (170/289, 58.8%) were commonly observed, followed by sore throat (49/289, 17.0%). Multivariate logistic regression analysis suggested that patients who were aged ≥ 65 years (OR: 2.725, 95% confidence interval [CI]: 1.317-5.636; P = 0.007), were male (OR: 1.878, 95% CI: 1.002-3.520, P = 0.049), had comorbid diabetes (OR: 3.314, 95% CI: 1.126-9.758, P = 0.030), cough (OR: 3.427, 95% CI: 1.752-6.706, P < 0.001), and/or diarrhea (OR: 2.629, 95% CI: 1.109-6.231, P = 0.028) on admission had a higher risk of severe disease. Moreover, stratification analysis indicated that male patients with diabetes were more likely to have severe COVID-19 (71.4% vs. 28.6%, χ2 = 8.183, P = 0.004). CONCLUSIONS: The clinical characteristics of those with severe and nonsevere COVID-19 were significantly different. The elderly, male patients with COVID-19, diabetes, and presenting with cough and/or diarrhea on admission may require close monitoring to prevent deterioration.


Assuntos
/diagnóstico , Adulto , China/epidemiologia , Comorbidade , Tosse , Estudos Transversais , Diarreia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco
13.
Int J Mol Sci ; 22(8)2021 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-33920519

RESUMO

Leymus chinensis is a perennial forage grass that has good palatability, high yield and high feed value, but seed dormancy is a major problem limiting the widespread cultivation of L. chinensis. Here, we performed transcriptomic and metabolomic analysis of hulled and de-hulled seeds of L. chinensis treated with or without GA3 to investigate the changes in gene and metabolites associated with dormancy release induced by GA3. The germination test revealed that the optimum concentration of GA3 for disruption of L. chinensis seed dormancy was 577 µM. A total of 4327 and 11,919 differentially expressed genes (DEGs) and 871 and 650 differentially abundant metabolites were identified in de-hulled and hulled seeds treated with GA3, respectively, compared with seeds soaked in sterile water. Most of the DEGs were associated with starch and sucrose metabolism, protein processing in the endoplasmic reticulum, endocytosis and ribosomes. Furthermore, isoquinoline alkaloid biosynthesis, tyrosine metabolism, starch and sucrose metabolism, arginine and proline metabolism, and amino sugar and nucleotide sugar metabolism were significantly enriched pathways. Integrative analysis of the transcriptomic and metabolomic data revealed that starch and sucrose metabolism is one of the most important pathways that may play a key role in providing carbon skeletons and energy supply for the transition of L. chinensis seeds from a dormant state to germination by suppressing the expression of Cel61a, egID, cel1, tpsA, SPAC2E11.16c and TPP2, enhancing the expression of AMY1.1, AMY1.2, AMY1.6 and GLIP5, and inhibiting the synthesis of cellobiose, cellodextrin, and trehalose while promoting the hydrolysis of sucrose, starch, cellobiose, cellodextrin, and trehalose to glucose. This study identified several key genes and provided new insights into the molecular mechanism of seed dormancy release induced by GA3 in L. chinensis. These putative genes will be valuable resources for improving the seed germination rate in future breeding studies.

14.
Shanghai Kou Qiang Yi Xue ; 30(1): 28-32, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33907775

RESUMO

PURPOSE: To qualitatively analyze the effect of 3D printed hydroxyapatite-gel (HAP-GEL) scaffold combined with bone marrow mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs) in repairing rabbit skull defect. METHODS: The third generation BMSCs and HUVECs were co-cultured with 3D printed HAP-GEL scaffold to construct tissue engineering bone. The rabbit model of skull defect was established and randomly divided into 4 groups. HAP-GEL stent, HAP-GEL stent + BMSCs and HUVECs cells were implanted respectively, and positive control (autologous bone tissue) and blank control were set up. Twelve weeks after operation, X-ray, cone-beam CT (CBCT) scan and H-E staining were performed to observe and analyze the changes of bone defect qualitatively. RESULTS:Twelve weeks after operation, imaging examination (X-ray and CBCT) showed that there was still obvious circular transmission in the blank control group, and the density was increased and the defect boundary was blurred in both HAP-GEL stent combined cell group and HAP-GEL group, among which the bone was continuous and the bone mineral density was the highest in HAP-GEL stent composite cell group, which was close to normal tissue. The results of H-E staining at twelve weeks showed that compared with the blank control group and the HAP-GEL group, the defect area of the HAP-GEL composite group was filled with new bone and bone-like tissue, the scaffold material was degraded and there was new bone formation inside the scaffold, and the bone repair effect was good, and the osteogenic effect was similar to that of the positive control group. CONCLUSIONS: 3D printed HAP-GEL scaffold + BMSCs + HUVECs cell complex has good osteogenic ability and biocompatibility with a good effect on repairing rabbit skull defect.


Assuntos
Células-Tronco Mesenquimais , Animais , Durapatita , Células Endoteliais da Veia Umbilical Humana , Osteogênese , Impressão Tridimensional , Coelhos , Crânio , Engenharia Tecidual , Tecidos Suporte
15.
Front Endocrinol (Lausanne) ; 12: 605736, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33912130

RESUMO

Purpose: Laron syndrome (LS) is a severe growth disorder caused by GHR gene mutation or post-receptor pathways defect. The clinical features of these patients collected in our present study were summarized, GHR gene variants were investigated and further in vitro functional verification was carried out. Methods: Four patients with LS were collected, their clinical characteristics were summarized, genomic DNA was extracted, and GHR gene was amplified and sequenced. GHR wild type (GHR-WT) and mutant GHR expression plasmids were constructed, and transiently transfected into HepG2 cells and HEK293T cells to observe the subcellular distribution of the GHR protein by immunofluorescence and to determine the expression of GHR and its post-receptor signaling pathway changes by Western blotting. Results: All of the four patients were male, and the median height was -4.72 SDS. Four GHR gene variants including c.587A>C (p.Y196S), c.766C>T (p.Q256*), c.808A>G (p.I270V) and c.1707-1710del (p.E570Afs*30) were identified, and the latter two were novel mutations. The results of mutant GHR plasmids transfection experiments and immunofluorescence assay showed that the subcellular distribution of GHR-Q256* and GHR-E570Afs*30 mutant proteins in HepG2 and HEK293T cells presented with a unique ring-like pattern, gathering around the nucleus, while GHR-Y196S mutant protein was evenly distributed on HepG2 cell membrane similar to GHR-WT. The GHR protein levels of HepG2 cells transiently transfected with GHR-Y196S, GHR-Q256* and GHR-E570Afs*30 were all significantly lower when compared with cells transfected with GHR-WT (P<0.05). Further mutant GHR post-receptor signal transduction investigation demonstrated that GH induced phosphorylated STAT5 levels of HepG2 cells transfected with three mutant plasmids were all significantly decreased in comparison with that of GHR-WT (P<0.05). Conclusions: Two novel GHR gene mutations (I270V and E570Afs*30) were found in our patients with LS. GHR mutations influenced the subcellular distribution and GHR protein levels, then led to the impaired post-receptor signal transduction, suggesting that the GHR mutations contributed to the pathological condition of LS patients.

16.
J Biomater Sci Polym Ed ; : 1-19, 2021 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-33858302

RESUMO

The bone healing defects resulting from bone disease remain a significant clinical challenge. The bone tissue engineering scaffolds combined with osteoinductive compounds represent an effective approach to overcome this challenge. In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element. Specifically, modified-diatomite (MDE) was synthesized by grafting polyethyleneimine (PEI) on the surface of diatomite via hydroxyl groups. The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively. Further, the mechanical strength, drug release, cytotoxicity and osteogenic activity analyses were carried out for the scaffold material. The FTIR and zeta potential analyses exhibited that the amino groups (-NH2) were grafted on MDE, and the surface potential of diatomite altered from -24 mV to 55 mV. Subsequently, the protein adsorption capacity and cytocompatibility of MDE were observed to be improved as compared to DE. The compressive strength was observed to be enhanced due to the addition of MDE. Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity. The findings obtained in this study reveal that the MDE-rhBMP-2/CS composite scaffold can be potentially used to promote the bone tissue regeneration.

17.
Artigo em Inglês | MEDLINE | ID: mdl-33705107

RESUMO

Formaldehyde (FA) and acetaldehyde (AcH) used as common chemicals in many fields are carcinogenic. The presently reported detection methods usually need expensive instruments, professional technicians, and time-consuming processes, and the detection sensitivity still needs further improvement. Herein, we report a highly effective fluorescence (FL) sensing film for FA and AcH based on naphthalimide derivative-infiltrated responsive SiO2 inverse opal photonic crystals (PCs), establishing a practically multiple-application detection platform for FA and AcH in air, aquatic products, and living cells. Nucleophilic addition products between the amine group of the naphthalimide derivative and aldehydes emit strong FL at ∼550 nm, realizing selective FL detection for FA and AcH. The emitted FL can be enhanced remarkably because of the slow photon effect of PCs, in which the FL wavelength is located at the stopband edge of PCs. A highly sensitive detection for FA and AcH with limits of detection of 10.6 and 7.3 nM, respectively, is achieved, increasing 3 orders of magnitude compared with that in the solution system. Additionally, the interconnected three-dimensional microporous inverse opal structure endows the sensor with a rapid response within 1 min. Furthermore, the as-prepared PC sensor can be reused by simple washing in an acidic aqueous solution. The sensing system can be used as a FL multi-detection platform for FA and AcH in air, aqueous solution, and living cells. This FL sensing approach based on small organic molecule-functionalized PCs is universally available to develop various sensors for target analytes by designing new functional organic compounds.

18.
J Cell Mol Med ; 25(7): 3400-3407, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33656264

RESUMO

MicroRNA-122 (miR-122) is known to be up-regulated by inflammation to exert a variety of biological functions in hepatocellular carcinoma (HCC)-derived human cell lines. Vitamin D receptor (VDR) is reported to regulate excessive oral keratinocytes apoptosis which compromises oral epithelial barrier in oral lichen planus (OLP). Although many studies have suggested that miR-122 is capable of regulating cell apoptosis, its effects on the development of OLP and VDR expression are still unclear. Herein, we demonstrate that miR-122 expression is increased in the epithelial layer of OLP. Mechanically, transcription factor nuclear factor-κB (NF-κB) selectively binds with κB element in the promoter of miR-122 to accelerate gene transcription. The up-regulation of miR-122 induces cell apoptosis in human oral keratinocytes (HOKs) by targeting VDR mRNA. In VDR knockout oral keratinocytes, miR-122 fails to improve caspase 3 activity and cleaved caspase 3 and poly(ADP-ribose) polymerase (PARP) levels. Moreover, VDR overexpression is able to reverse lipopolysaccharide (LPS)- or activated CD4+ T cell-induced miR-122 up-regulation and ameliorate miR-122-stimulated caspase 3 activity. Collectively, our results suggest that miR-122 promotes oral keratinocytes apoptosis in OLP through decreasing VDR expression.

19.
ACS Nano ; 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33689315

RESUMO

Because of translational symmetry, electromagnetic fields confined within 2D periodic optical structures can be represented within the first Brillouin zone (BZ). In contrast, the wavevectors of scattered electromagnetic fields outside the lattice are constrained by the 3D light cone, the free-photon dispersion in the surrounding medium. Here, we report that light-cone surface lattice resonances (SLRs) from plasmonic nanoparticle lattices can be used to observe the radiated electromagnetic fields from extended BZ edges. Our coupled dipole radiation theory reveals how lattice geometry and induced surface plasmon dipole orientation affect angular distributions of the radiated fields. Using dye molecules as local dipole emitters to excite the light-cone SLR modes, we experimentally identified high-order BZ edges by directional, in-plane lasing emission. These results provide insight into nanolaser architectures that can emit at multiple wavelengths and in-plane directions simply by rotating the nanocavity lattice.

20.
J Biophotonics ; : e202000478, 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33768709

RESUMO

The in vivo hemodynamic impact of sodium nitroprusside (SNP), a widely used antihypertensive agent, has not been well studied. Here, we applied functional optical-resolution photoacoustic microscopy (OR-PAM) to study the hemodynamic responses to SNP in mice in vivo. As expected, after the application of SNP, the systemic blood pressure (BP) was reduced by 53%. The OR-PAM results show that SNP induced an arterial vasodilation of 24% and 23% in the brain and skin, respectively. A weaker venous vasodilation of 9% and 5% was also observed in the brain and skin, respectively. The results show two different types of blood oxygenation response. In mice with decreased blood oxygenation, the arterial and venous oxygenation was respectively reduced by 6% and 13% in the brain, as well as by 7% and 18% in the skin. In mice with increased blood oxygenation, arterial and venous oxygenation was raised by 4% and 22% in the brain, as well as by 1% and 9% in the skin. We observed venous change clearly lagged the arterial change in the skin, but not in the brain. Our results collectively show a correlation among SNP induced changes in systemic BP, vessel size and blood oxygenation.

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