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1.
Sheng Li Xue Bao ; 73(3): 407-422, 2021 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-34230943

RESUMO

As the two essential components, the white matter and gray matter compose the central nervous system of the brain. Widely known that axons of neurons mainly form the white matter, and these formed nerve fibers are responsible for transmitting information among various brain regions to achieve the coordinated operation of the entire brain. Early research on the white matter could only be done by dissecting living animals or human cadavers, until Basser et al. proposed diffusion tensor imaging (DTI) technology in 1994, which could detect the diffusion characteristics of water in the brain in vivo noninvasively. Accordingly, this technology could be applied to investigate the diffusion movement of water in white matter to obtain the information of direction and micro-anatomy of white matter fiber bundles. With the advancement on the display and analysis of the anatomical structure of white matter fiber bundles, the exploration of microscopic pathological changes, and the assistance of clinical diagnosis and neurophysiological research, DTI technology has become one of the most popular topics in brain science research. Chronic pain refers to pain lasting more than three months, which not only seriously affects the patient's physical and social functions, but also dramatically reduces the quality of life. It was reported that long-term pain stimulation might cause pathological remodeling of the central nervous system, and abnormalities in white matter were found in imaging examinations of patients with chronic pain. This review introduces the quantitative analysis methods of white matter fiber bundle microstructure based on DTI and its application in chronic pain, and further discusses the application value of DTI technology on clinical research of chronic pain.


Assuntos
Dor Crônica , Substância Branca , Animais , Encéfalo , Dor Crônica/diagnóstico por imagem , Imagem de Tensor de Difusão , Humanos , Qualidade de Vida , Substância Branca/diagnóstico por imagem
2.
J Dent Educ ; 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34212371

RESUMO

OBJECTIVES: The purpose of this study was to determine how students perceived the use of a 3D video microscope (3D VM), in comparison to conventional methods, for providing instruction on fixed prosthodontics procedures and issuing feedback on student performance. METHODS: From May until December of 2020, University at Buffalo School of Dental Medicine's second-year students were introduced to fixed prosthodontics. During May and June, a 3D VM was used to live-stream psychomotor skill demonstrations via a web-based software. After each procedure, students were asked to complete a short survey regarding their experience with the training. From August until December 2020, students received feedback via: 1. The conventional method, in which faculty use magnification loupes to assess student's perfromance. 2. A 3D VM. Irrespective of the method used, feedback was given following the same defined rubrics. At the conclusion of the semester, students completed a survey comparing their experiences in receiving instruction and feedback. The data were gathered and analyzed descriptively. The chi-square test (α = .05) was used to gauge if students' gender had an impact on their perception of feedback methods. RESULTS: More than 94% of students participated in the surveys. The majority of students had a positive reaction toward the use of the 3D VM for live web-based training. In regard to receiving feedback, 63% opined that the 3D VM was more effective than the conventional method. Additionally, 80% of students felt that the 3D VM was effective in maintaining physical distance during skill evaluations. CONCLUSION: This study examined the use of a 3D VM for teaching psychomotor skills and providing student feedback and compared it to the conventional method. It was found that the use of the 3D VM was perceived positively by students. Gender had no significant impact on students' attitudes toward the teaching and feedback methods.

3.
Chin Med Sci J ; 36(2): 103-109, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34231458

RESUMO

Objective Chronic cardiovascular diseases induced by long-term poor blood glucose control are the main cause of death in patients with type 2 diabetes mellitus (T2DM). Previous researches report that methylenetetrahydrofolate reductase gene (MTHFR) polymorphisms might influence the occurrence of coronary heart disease (CHD) in T2DM patients. The purpose of this study was to evaluate whether MTHFR C677T and A1298C mutations are associated with the risk of CHD in T2DM patients. Methods A total of 197 subjects with T2DM were studied, of which 95 patients with CHD. The genotypes of MTHFR C677T and A1298C were analyzed by using dideoxy chain-termination method, and compared between patients with CHD and those without CHD. Results We found that the frequency of the 677T allele was significantly higher in T2DM patients with CHD than those without CHD (P=0.011). However, there was no significant difference in any of the examined haplotypes between T2DM patients with and without CHD. Furthermore, the 677T allele was associated with a higher risk of CHD development in diabetic patients with lower homocysteine (Hcy) levels (≤15 µmol/L) (P=0.006), while no effect of MTHFR gene polymorphism on the incidence of CHD was found in patients with higher Hcy levels (>15 µmol/L) (P=0.491). Conclusion The MTHFR C677T gene polymorphism is associated with the risk of CHD of diabetic patients and could be used as an effective marker for CHD in Chinese diabetic populations with normal Hcy levels.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34208824

RESUMO

Most of the fatalities and injuries of oilfield workers result from inadequate protection and comfort by their clothing under various work hazards and ambient environments. Both the thermal protective performance and thermo-physiological comfort performance of textile fabrics used in clothing significantly contribute to the mitigation of workers' skin burns and heat-stress-related deaths. This study aimed to apply the ANN modeling approach to analyze clothing performance considering the wearers' sweat moisture and the microclimate air gap that is generated in between their body and clothing. Firstly, thermal protective and thermo-physiological comfort performance of fire protective textiles used in oilfield workers' clothing were characterized. Different fabric properties (e.g., thickness, weight, fabric count), thermal protective performance, and thermo-physiological comfort performance were measured. The key fabric property that affects thermal protective and thermo-physiological performance was identified as thickness by statistical analysis. The ANN modeling approach could be successfully implemented to analyze the performance of fabrics in order to predict the performance more conveniently based on the fabric properties. It is expected that the developed models could inform on-duty oilfield workers about protective and thermo-physiological comfort performance and provide them with occupational health and safety.

5.
Shock ; 56(2): 229-236, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34276038

RESUMO

BACKGROUND: Extracellular cold-inducible RNA-binding protein (eCIRP) acting as a novel damage-associated molecular pattern molecule promotes systemic inflammatory responses, including neuroinflammation in cerebral ischemia. We aimed to observe the changes of serum eCIRP and evaluate whether the increased serum eCIRP was associated with the severity and prognosis in patients with restoration of spontaneous circulation (ROSC). METHODS: A total of 73 patients after ROSC were divided into non-survivor (n = 48) and survivor (n = 25) groups based on 28-day survival. Healthy volunteers (n = 25) were enrolled as controls. Serum eCIRP, procalcitonin (PCT), the pro-inflammatory mediators tumor necrosis factor (TNF)-α, interleukin-6 (IL)-6 and high mobility group protein (HMGB1), the neurological damage biomarkers neuron-specific enolase (NSE), and soluble protein 100ß (S100ß) were measured on days 1, 3, and 7 after ROSC. Clinical data and laboratory findings were collected, and the Sequential Organ Failure Assessment (SOFA) score and Acute Physiology and Chronic Health Evaluation (APACHE II) were calculated concurrently. Cerebral performance category scores on day 28 after ROSC were recorded. RESULTS: Serum eCIRP, IL-6, TNF-α, PCT, and HMGB1, NSE and S100ß were significantly increased within the first week after ROSC. The increased levels of eCIRP were positively correlated with IL-6, TNF-α, lactate, NSE, S100ß, CPR time, SOFA score, APACHE II score, and HMGB1 after ROSC. Serum eCIRP on days 1, 3, and 7 after ROSC could predict 28-day mortality and neurological prognosis. Serum eCIRP on day 3 after ROSC had a biggest AUC [0.862 (95% CI: 0.741-0.941)] for 28-day mortality and a biggest AUC [0.807 (95% CI: 0.630-0.981)] for neurological prognosis. CONCLUSIONS: Systemic inflammatory response with increased serum eCIRP occurred in patients after ROSC. Increased eCIRP level was positively correlated with the aggravation of systemic inflammatory response and the severity after ROSC. Serum eCIRP serves as a potential predictor for 28-day mortality and poor neurological prognosis after ROSC.

6.
Cell Res ; 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34267352

RESUMO

Long noncoding RNAs (lncRNAs) are emerging as a new class of important regulators of signal transduction in tissue homeostasis and cancer development. Liquid-liquid phase separation (LLPS) occurs in a wide range of biological processes, while its role in signal transduction remains largely undeciphered. In this study, we uncovered a lipid-associated lncRNA, small nucleolar RNA host gene 9 (SNHG9) as a tumor-promoting lncRNA driving liquid droplet formation of Large Tumor Suppressor Kinase 1 (LATS1) and inhibiting the Hippo pathway. Mechanistically, SNHG9 and its associated phosphatidic acids (PA) interact with the C-terminal domain of LATS1, promoting LATS1 phase separation and inhibiting LATS1-mediated YAP phosphorylation. Loss of SNHG9 suppresses xenograft breast tumor growth. Clinically, expression of SNHG9 positively correlates with YAP activity and breast cancer progression. Taken together, our results uncover a novel regulatory role of a tumor-promoting lncRNA (i.e., SNHG9) in signal transduction and cancer development by facilitating the LLPS of a signaling kinase (i.e., LATS1).

7.
Artigo em Inglês | MEDLINE | ID: mdl-34204018

RESUMO

Despite near universal health insurance coverage in China, populations with low incomes may still face barriers in access and utilization of affordable health care. We aimed to identify the likelihood of forgone medical care due to cost by surveying individuals from the community to assess: (1) The percent with forgone medical care due to cost; and (2) Factors associated with forgone medical care due to cost. Surveys conducted (2016-2017) in Mandarin included demographic and medical care utilization-related items. Theoretically-informed, fully-adjusted analyses were employed. Approximately 94% of respondents had health insurance, which is somewhat similar to national estimates. Overall, 24% of respondents resided in rural areas, with 18% having less than a high school education, and 49% being male. More than 36% reported forgone medical care due to cost in the past 12 months. In fully-adjusted analyses, having lower education, generally not being satisfied with the commute to the hospital, and being a resident of a province with a lower density of physicians were associated with forgone medical care. Cost-related disparities in the access and utilization of needed medical care persist, even with near universal health insurance, which may be due to one's satisfaction with travel time to healthcare and other community assets.


Assuntos
Acesso aos Serviços de Saúde , Determinantes Sociais da Saúde , China , Feminino , Hospitais , Humanos , Seguro Saúde , Masculino , Pobreza , Fatores Socioeconômicos
8.
Artigo em Inglês | MEDLINE | ID: mdl-34204145

RESUMO

Depressive symptoms are a common mental health problem among adolescents, which may affect their physical and mental health development and impose heavy burdens on individual families and society. This study aimed to examine the associations between sleep duration, academic pressure, and depressive symptoms among Chinese adolescents and to construct the mediation model to explore the mediating effect of sleep duration. The data are from the China Family Panel Studies (CFPS). Methodologically, the aforementioned associations were explored by constructing a structural equation model and applying multivariate multilevel logistic regression. In this study, we found that approximately 6.49% of the 3724 Chinese adolescents had depressive symptoms. Sleep duration of <6 h/night (OR = 2.39, 95%CI = 1.33-4.32) and high/maximum academic pressure (high: OR = 1.43, 95%CI = 1.02-1.99; maximum: OR = 2.43, 95%CI = 1.58-3.73) were both associated with an increased risk of depressive symptoms in adolescents. Meanwhile, the multiplicative interaction between sleep duration and academic pressure was significantly associated with depressive symptoms in adolescents (p < 0.001). The sleep duration played a partial mediating role in the relationship between academic pressure and depressive symptoms (a*b = 0.006, 95%BootCI = 0.001-0.012). Our study highlights that it is essential to mitigate the academic pressure of adolescents to increase their sleep duration and further reduce the occurrence of depressive symptoms by adopting corresponding preventive measures.


Assuntos
Depressão , Saúde Mental , Adolescente , Grupo com Ancestrais do Continente Asiático , China/epidemiologia , Depressão/epidemiologia , Humanos , Sono
9.
Medicine (Baltimore) ; 100(27): e26557, 2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34232198

RESUMO

ABSTRACT: Radiomics transforms the medical images into high-dimensional quantitative features and provides potential information about tumor phenotypes and heterogeneity. We conducted a retrospective analysis to explore and validate radiomics model based on contrast-enhanced computed tomography (CECT) to predict recurrence of locally advanced oesophageal squamous cell cancer (SCC) within 2 years after trimodal therapy. This study collected CECT and clinical data of consecutive 220 patients with pathology-confirmed locally advanced oesophageal SCC (154 in the training cohort and 66 in the validation cohort). Univariate statistical test and the least absolute shrinkage and selection operator method were performed to select the optimal radiomics features. Logistic regression was conducted to build radiomics model, clinical model, and combined model of both the radiomics and clinical features. Predictive performance was judged by the area under receiver operating characteristics curve (AUC), accuracy, and F1-score in the training and validation cohorts. Ten optimal radiomics features and/or 7 clinical features were selected to build radiomics model, clinical model, and the combined model. The integrated model of radiomics and clinical features was superior to radiomics model or clinical model in predicting recurrence of locally advanced oesophageal SCC within 2 years in the training (AUC: 0.879 vs 0.815 or 0.763; accuracy: 0.844 vs 0.773 or 0.740; and F1-score: 0.886 vs 0.839 or 0.815, respectively) and validation (AUC: 0.857 vs 0.720 or 0.750; accuracy: 0.788 vs 0.700 or 0.697; and F1-score: 0.851 vs 0.800 or 0.787, respectively) cohorts. The combined model of radiomics and clinical features shows better performance than the radiomics or clinical model to predict the recurrence of locally advanced oesophageal SCC within 2 years after trimodal therapy.


Assuntos
Neoplasias Esofágicas/diagnóstico , Carcinoma de Células Escamosas do Esôfago/diagnóstico , Recidiva Local de Neoplasia/diagnóstico , Estadiamento de Neoplasias , Tomografia Computadorizada por Raios X/métodos , Terapia Combinada , Neoplasias Esofágicas/terapia , Carcinoma de Células Escamosas do Esôfago/terapia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC , Estudos Retrospectivos , Fatores de Tempo
10.
Pharm Dev Technol ; : 1-10, 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34238120

RESUMO

Curcumin (CUR) shows great potential in the management of alcohol-use disorders. However, the hydrophobicity and poor oral bioavailability result in the limited therapeutic efficacy of CUR against alcohol-induced tissue injury. Here, self-assembled Soluplus® micelles (Ms) were developed for the enhanced oral delivery of CUR. CUR-loaded Soluplus® micelles (CUR-Ms) were prepared using a thin-film hydration method and these micelles displayed nearly spherical shape with an average size of 62.80 ± 1.29 nm. CUR in micelles showed the greater stability, solubility and dissolution than free CUR. With the increased water solubility of CUR-Ms and P glycoprotein inhibition of Soluplus®, the absorption rate constant (Ka) and apparent permeability coefficient (Papp) of CUR-Ms in intestines was respectively 3.50 and 4.10 times higher than that of free CUR. Pharmacokinetic studies showed that CUR-Ms significantly improved the oral bioavailability of CUR. Specifically, the AUC0-∞ and Cmax of CUR-Ms were increased by 9.45 and 47.38 folds compared to free CUR, respectively. In mice with alcohol-induced tissue injury, the oral administration of CUR-Ms greatly reduced oxidative stress, and significantly defended liver and gastric mucosa from alcoholic damages. The results demonstrated CUR-Ms with good oral bioavailability could represent a promising strategy for the management of alcohol-induced tissue injury.

11.
AJR Am J Roentgenol ; : 1-12, 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34259544

RESUMO

OBJECTIVE. The purpose of our study was to develop a radiomics model based on preoperative MRI and clinical information for predicting recurrence-free survival (RFS) in patients with advanced high-grade serous ovarian carcinoma (HGSOC). MATERIALS AND METHODS. This retrospective study enrolled 117 patients with HGSOC, including 90 patients with recurrence and 27 without recurrence; 1046 radiomics features were extracted from T2-weighted images and contrast-enhanced T1-weighted images using a manual segmentation method. L1 regularization-based least absolute shrinkage and selection operator (LASSO) regression was performed to select features, and the synthetic minority oversampling technique (SMOTE) was used to balance our dataset. A support vector machine (SVM) classifier was used to build the classification model. To validate the performance of the proposed models, we applied a leave-one-out cross-validation method to train and test the classifier. Cox proportional hazards regression, Harrell concordance index (C-index), and Kaplan-Meier plots analysis were used to evaluate the associations between radiomics signatures and RFS. RESULTS. The fusion radiomics-based model yielded a significantly higher AUC value of 0.85 in evaluating RFS than the model using contrast-enhanced T1-weighted imaging features alone or T2-weighted imaging features alone (AUC = 0.79 and 0.74 and p = .02 and .01, respectively). Kaplan-Meier survival curves showed significant differences between high and low recurrence risk in patients with HGSOC by different models. The fusion model combining radiomics features and clinical information showed higher performance than the clinical model (C-index = 0.62 and 0.60, respectively). CONCLUSION. The proposed MRI-based radiomics signatures may provide a potential way to develop a prediction model and can help identify patients with advanced HGSOC who have a high risk of recurrence.

12.
J Med Virol ; 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34255379

RESUMO

We have developed a rapid and simple a magnetic chemiluminescence enzyme immunoassay on the Real Express-6 analyzer, which could simultaneously detect IgG and IgM antibodies against SARS-CoV-2 virus in human blood within 18 minutes, which could detect clinical studies to verify its clinical efficacy. We selected blood samples from 185 COVID-19 patients confirmed by PCR and 271 negative patients to determine the clinical detection sensitivity, specificity, stability and precision of this method. Meanwhile, we also surveyed the dynamic variance of viral antibodies during SARS-CoV-2 infection. This rapid immunoassay test has huge potential benefits for rapid screening of SARS-CoV-2 infection, and may be help clinical drug and vaccine development. This article is protected by copyright. All rights reserved.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34205544

RESUMO

This study aimed to evaluate the relationship between psychosocial variables (peer support, parental support, autonomous motivation, and controlled motivation), availability of physical activity resources in a neighborhood environment, and out-of-school moderate to vigorous physical activity (MVPA) among Chinese adolescents. The questionnaire of Family Life, Activity, Sun, Health, and Eating (FLASHE) Study was used to collect information on demographics, socioeconomic status, psychosocial variables, available physical activity resources in the neighborhood environment, and minutes of out-of-school MVPA. ANOVA analysis and multiple regression analysis were performed. The mean age of the 3833 adolescents included in our analysis was 14.7 years old (SD = 1.7). Peer support (b = 9.35, 95% CI: 7.55-11.15), autonomous motivation (b = 6.46, 95% CI: 4.09-8.82), parental support (b = 3.90, 95% CI: 1.75-6.07), and availability of physical activity resources in neighborhood environment (b = 3.18, 95% CI: 1.99-4.36) were significantly associated with out-of-school MVPA (p < 0.05). Controlled motivation was insignificantly related to minutes of out-of-school MVPA. Boys spent more time on out-of-school MVPA than girls (p < 0.001) and had a high level of peer support, parental support, and motivation (p < 0.05). Our findings suggest that interventions targeting the out-of-school MVPA among Chinese adolescents should focus on the psychosocial variables and neighborhood environment.


Assuntos
Exercício Físico , Características de Residência , Adolescente , China , Estudos Transversais , Feminino , Humanos , Masculino , Motivação , Instituições Acadêmicas
14.
BMC Ophthalmol ; 21(1): 283, 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34289821

RESUMO

BACKGROUNDS: Myopia has become a global public health problem. Children with early onset of myopia are at particular risk of complications associated with myopia. Younger children and children with greater initial myopic refractive errors are at a greater risk of myopia progression. Therefore, it is essential to identify subjects at high risk of developing myopia to facilitate myopia prevention in the early stage, especially during the preschool period. The purpose of this study was to determine whether premyopia and myopia in preschool children can be predicted by easily obtainable parameters. METHODS: Data was collected in a population-based cohort. Comprehensive examinations included height, weight, refraction, axial length (AL), and corneal radius of curvature (CR), with a follow-up of 2 years. Parental myopia history was obtained from a questionnaire. Myopia was defined as spherical equivalent (SE) ≤ - 0.50 D. Premyopia was defined as - 0.50 D < SE ≤ + 0.75 D. Multivariate linear regression models were fitted to determine the associations between these parameters at baseline and future SE. To predict premyopia and myopia, Cox proportional hazard regression analysis coupled with a nomogram was used. RESULTS: A total of 830 children (433 boys and 397 girls) were included (40.83 ± 3.43 months old at baseline). A significantly negative relationship was observed in the multivariate analysis between baseline AL, AL/CR, two myopic parents, and the future SE after adjusting for age and gender (coefficient = - 0.291, coefficient = - 5.791, coefficient = - 0.273, respectively, both p <  0.001). Higher baseline AL, AL/CR (hazard ratio (HR) = 4.916, HR = 2.979, respectively, comparing the top quartile with the bottom quartile, both p <  0.001) and two myopic parents (HR = 1.756, compared to no myopic parents, p = 0.001) were associated with a higher risk of future onset of premyopia. From the nomogram, AL/CR was found to have the most enormous effect on survival. Different baseline AL and AL/CR values (both Log Rank p <  0.001) had different survival curves. CONCLUSIONS: AL and AL/CR could be used as obtainable indicators for identifying subjects at high risk of developing premyopia and myopia in young preschool children.


Assuntos
Comprimento Axial do Olho , Miopia , Pré-Escolar , China/epidemiologia , Córnea , Progressão da Doença , Feminino , Humanos , Masculino , Miopia/epidemiologia , Refração Ocular
15.
Bioorg Chem ; 115: 105187, 2021 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-34303037

RESUMO

BACKGROUND: Guanxin V (GXV) has been widely used to treat ventricular remodeling (VR) in clinical practice in China. However, the underlying mechanisms are currently still lack. METHODS: A systematic pharmacology-based strategy was utilized for predicting the synergistic pharmacological mechanisms of GXV in VR. The active compounds of GXV were selected and then the potential targets of these compounds contained in GXV and VR were successively identified. Then, after networks were constructed, DAVID was applied to functional enrichment. Moreover, the key findings were validated though molecular docking and molecular biology experiments. RESULTS: A total of 119 active components in GXV and 169 potential targets shared between CAD and VR were obtained. The results of functional enrichment indicated that several biological processes and signaling pathways, mainly cell apoptosis and fibrosis. Finally, we discovered GXV produced marked anti-apoptosis and anti-fibrosis effects in VR though Caspase-3 and TGF-ß1. CONCLUSION: GXV could relieve and reverse VR through anti-apoptosis and anti-fibrosis effects predicted by systematic pharmacology and validated by molecular docking and molecular experiments. Our study deepens the understanding of the molecular mechanisms of GXV in treating VR.

16.
Dis Markers ; 2021: 5552614, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34306255

RESUMO

Background: GA-binding protein A (GABPA), a transcription factor, is broadly involved in physiological and pathological processes. Several studies have investigated the relationship between GABPA expression level and outcomes of various malignancies. However, the function and clinicopathological significance of GABPA in endometrial carcinoma (EC) remain obscure. Methods: The GABPA mRNA expression in EC tissues and adjacent nonneoplastic tissues in the TCGA database was involved in our study. The protein expression of GABPA in 107 EC tissues and 15 normal endometrial tissues was detected by immunohistochemistry. Results: The GABPA expression was significantly downregulated in EC tissues compared with its expression in normal tissues (P < 0.001). The expression of GABPA was markedly correlated with type II EC (P < 0.01) and grade 3 EC (P < 0.05). A tendency has been observed that patients with low GABPA levels had relatively poorer overall survival (OS) (P = 0.036) and disease-free survival (DFS) (P = 0.016) than patients with high GABPA levels. The multivariate Cox proportional hazard model showed that lower expression of GABPA was an independent poor prognostic factor for OS (P = 0.043) and DFS (P = 0.045). Similar correlation between low expression levels of GABPA and unfavorable prognosis has also been found in type II or grade 3 EC. IHC analysis showed EC tissues had low expression of GABPA, which indicated relatively poor prognosis. Moreover, we identified that the GABPA mRNA expression was negatively correlated with its methylation level (R = -0.2512, P < 0.001) which is one of the mechanisms for the silencing of GABPA gene. Conclusion: GABPA may act as an independent predictor of clinical prognosis and serve as a potential target gene for EC therapy.

17.
Environ Res ; 201: 111613, 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34224714

RESUMO

The current industrial structure of eco-fragile areas fails to reverse the trend of the deterioration of the ecological environment and severely affects the sustainable development of eco-fragile areas, so conducting scientific and technological studies of ecological industries and ecological products helps eco-fragile areas recover their degraded ecosystem. The results of a statistical analysis of 159 related documents show: (1) the number of documents published rises wave upon wave in time series, which indicates that this area of study is still at the stage of rising; (2) research contents concentrate on five dimensions-theoretical research, technology research and development, model building, demonstration and promotion, and efficiency analysis, among which theoretical research chiefly discusses environmental costs and effects of ecological industries, regional organizational layout, industrial chain construction, market supply and demand of biological products, discrimination of the nature of biological products, and value assessment and realization of biological products; (3) study areas are largely distributed in the Loess Plateau area in the north and karst areas in the south in China, which suggests that studies of biological industries and biological products in eco-fragile areas concentrate on arid and semi-arid areas and engineered arid areas. Furthermore, from five aspects-theoretical study, technology research and development, model building, experiment and demonstration, and monitoring and evaluation, this study reveals cutting-edge theories about ecological industries and ecological products in eco-fragile areas. At last, it is found that comprehensive theoretical research of the market circulation of ecological products in eco-fragile areas and the research and development of key technologies to enhance the value of ecological products lag behind, and it is necessary to deepen the studies about the whole industry chain development for ecological industries, the valuation of ecological effect, the standardization of ecological industry management, the circulation mechanism of ecological products, brand building and other aspects.

19.
Int J Methods Psychiatr Res ; : e1887, 2021 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-34291527

RESUMO

OBJECTIVES: This study aimed to explore whether game training could improve cognitive functioning and depression symptoms in the elderly affected by mild cognitive impairment (MCI). METHODS: A non-blinded randomized controlled trial was conducted. Participants were 72 patients with MCI and depression from a nursing home in Wuhan. Participants were randomized to either the intervention group or the control group (n = 36 each). The intervention group received regular nursing care plus game training for 50 min, three times per week for 8 weeks, whereas the control group received only regular nursing care during the same research period. Cognitive functioning and depression symptoms were tested in both groups at baseline and at the end of the 8-week intervention. We used the Montreal Cognitive Assessment and the 15-item Geriatric Depression Scale to assess cognitive functioning and depression symptoms, respectively. RESULTS: The 8-week game training intervention significantly improved the cognitive and depression scores when compared with the control group and baseline scores (p < 0.05). No significant difference was observed in the control group (p > 0.05). CONCLUSIONS: Our results suggest that the implementation of game training can improve the cognitive functioning and depression symptoms of the elderly with MCI, indicated that can be widely used.

20.
Chemosphere ; 285: 131453, 2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34246093

RESUMO

In this paper, the effects of several groundwater components (heavy metals, inorganic anions, and organics) on the cytotoxicity of nanoscale zero-valent iron (NZVI) towards Escherichia coli (E. coli) under aerobic/anaerobic conditions were studied. The results showed that NZVI exhibited much higher toxicity in anaerobic conditions than aerobic conditions. Under the state of air-saturation, corrosion of NZVI occurred rapidly, at the same time, it could stably and continuously generate Fe (Ⅱ) and trigger reactive oxygen species (ROS), which led to oxidative stress in E. coli. While in the deareated state, the TEM images showed that the integrity of the cell membrane was destroyed, which validated that the main mechanism of NZVI cytotoxicity was the rapid membrane damage of E. coli. The presence of Cr (Ⅵ) reduced the toxicity of NZVI through oxidation-reduction with NZVI, especially under anaerobic conditions. In contrast, the presence of Cd (Ⅱ) could be adsorbed onto NZVI to increase the cytotoxicity of NZVI. The presence of phosphate and humic acid greatly improved the survival rate of E. coli through the complex reaction with Fe (Ⅱ), especially under aerobic conditions. On the one hand, the formed Fe (II)-phosphate/humic acid complex could reduce the production of ROS. On the other hand, the complex accumulated on the outer surface of E. coli cells could provide steric hindrance to impede the contact between NZVI and cell. These findings were crucial for practical significance to evaluate environmental risk during the groundwater remediation process by using NZVI.

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